TY  - JOUR
AU  - Talevski, Trajce
AU  - Talevska-Leshoska, Aleksandra
AU  - Pejoski, Elena
AU  - Pejin, Boris
AU  - Machalowski, Tomasz
AU  - Wysokowski, Marcin
AU  - Tsurkan, Mikhail, V
AU  - Petrova, Olga
AU  - Sivkov, Viktor
AU  - Martinović, Rajko
AU  - Pantović, Snežana
AU  - Khrunyk, Yuliya
AU  - Trylis, Volodymyr
AU  - Fursov, Andriy
AU  - Djurović, Mirko
AU  - Jesionowski, Teofil
AU  - Ehrlich, Hermann
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1324
AB  - Studies on the identification, properties and function of chitin in sponges (Porifera), which are recognized as the first multicellular organisms on Earth, continue to be of fundamental scientific interest. The occurrence of chitin has so far been reported in 21 marine sponge species and only in two inhabiting fresh water. In this study, we present the discovery of alpha-chitin in the endemic demosponge Ochridaspongia rotunda, found in Lake Ohrid, which dates from the Tertiary. The presence of chitin in this species was confirmed using special staining, a chitinase test, FTIR, Raman and NEXAFS spectroscopy, and electrospray ionization mass spectrometry (ESIMS). In contrast to the case of marine sponges, chitin in O. rotunda has been found only within its holdfast, suggesting a role of chitin in the attachment of the sponge to the hard substratum. Isolated fibrous matter strongly resemble the shape and size of the sponge holdfast with membrane-like structure.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937)
EP  - 1194
SP  - 1187
VL  - 162
DO  - 10.1016/j.ijbiomac.2020.06.247
ER  - 

@article{
author = "Talevski, Trajce and Talevska-Leshoska, Aleksandra and Pejoski, Elena and Pejin, Boris and Machalowski, Tomasz and Wysokowski, Marcin and Tsurkan, Mikhail, V and Petrova, Olga and Sivkov, Viktor and Martinović, Rajko and Pantović, Snežana and Khrunyk, Yuliya and Trylis, Volodymyr and Fursov, Andriy and Djurović, Mirko and Jesionowski, Teofil and Ehrlich, Hermann",
year = "2020",
abstract = "Studies on the identification, properties and function of chitin in sponges (Porifera), which are recognized as the first multicellular organisms on Earth, continue to be of fundamental scientific interest. The occurrence of chitin has so far been reported in 21 marine sponge species and only in two inhabiting fresh water. In this study, we present the discovery of alpha-chitin in the endemic demosponge Ochridaspongia rotunda, found in Lake Ohrid, which dates from the Tertiary. The presence of chitin in this species was confirmed using special staining, a chitinase test, FTIR, Raman and NEXAFS spectroscopy, and electrospray ionization mass spectrometry (ESIMS). In contrast to the case of marine sponges, chitin in O. rotunda has been found only within its holdfast, suggesting a role of chitin in the attachment of the sponge to the hard substratum. Isolated fibrous matter strongly resemble the shape and size of the sponge holdfast with membrane-like structure.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937)",
pages = "1194-1187",
volume = "162",
doi = "10.1016/j.ijbiomac.2020.06.247"
}

Talevski, T., Talevska-Leshoska, A., Pejoski, E., Pejin, B., Machalowski, T., Wysokowski, M., Tsurkan, M. V., Petrova, O., Sivkov, V., Martinović, R., Pantović, S., Khrunyk, Y., Trylis, V., Fursov, A., Djurović, M., Jesionowski, T.,& Ehrlich, H.. (2020). Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937). in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 162, 1187-1194.
https://doi.org/10.1016/j.ijbiomac.2020.06.247

Talevski T, Talevska-Leshoska A, Pejoski E, Pejin B, Machalowski T, Wysokowski M, Tsurkan MV, Petrova O, Sivkov V, Martinović R, Pantović S, Khrunyk Y, Trylis V, Fursov A, Djurović M, Jesionowski T, Ehrlich H. Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937). in International Journal of Biological Macromolecules. 2020;162:1187-1194.
doi:10.1016/j.ijbiomac.2020.06.247 .

Talevski, Trajce, Talevska-Leshoska, Aleksandra, Pejoski, Elena, Pejin, Boris, Machalowski, Tomasz, Wysokowski, Marcin, Tsurkan, Mikhail, V, Petrova, Olga, Sivkov, Viktor, Martinović, Rajko, Pantović, Snežana, Khrunyk, Yuliya, Trylis, Volodymyr, Fursov, Andriy, Djurović, Mirko, Jesionowski, Teofil, Ehrlich, Hermann, "Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937)" in International Journal of Biological Macromolecules, 162 (2020):1187-1194,
https://doi.org/10.1016/j.ijbiomac.2020.06.247 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Talevska, Aleksandra
AU  - Ciric, Ana
AU  - Glamočlija, Jasmina
AU  - Nikolic, Milos
AU  - Talevski, Trajce
AU  - Soković, Marina
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/806
AB  - The anti-quorum sensing activities towards the bacterium Pseudomonas aeruginosa PA01 (pyocyanin production, biofilm formation and twitching and flagella motility) of two crude extracts (methanol and acetone) of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) were evaluated in vitro for the first time. Both extracts demonstrated P. aeruginosa pyocyanin inhibitory activity, reducing its production for 49.90% and 42.44%, respectively. In addition, they both showed higher anti-biofilm activity (48.29% and 53.99%, respectively) than ampicillin (30.84%). Finally, O. rotunda extracts effectively reduced twitching and flagella motility of P. aeruginosa. Taken all together, these results suggest that endemic sponge species from the oldest lake in Europe may offer novel bioactive natural products with promising medicinal potential towards P. aeruginosa infections.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa
EP  - 2333
IS  - 24
SP  - 2330
VL  - 28
DO  - 10.1080/14786419.2014.934239
ER  - 

@article{
author = "Pejin, Boris and Talevska, Aleksandra and Ciric, Ana and Glamočlija, Jasmina and Nikolic, Milos and Talevski, Trajce and Soković, Marina",
year = "2014",
abstract = "The anti-quorum sensing activities towards the bacterium Pseudomonas aeruginosa PA01 (pyocyanin production, biofilm formation and twitching and flagella motility) of two crude extracts (methanol and acetone) of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) were evaluated in vitro for the first time. Both extracts demonstrated P. aeruginosa pyocyanin inhibitory activity, reducing its production for 49.90% and 42.44%, respectively. In addition, they both showed higher anti-biofilm activity (48.29% and 53.99%, respectively) than ampicillin (30.84%). Finally, O. rotunda extracts effectively reduced twitching and flagella motility of P. aeruginosa. Taken all together, these results suggest that endemic sponge species from the oldest lake in Europe may offer novel bioactive natural products with promising medicinal potential towards P. aeruginosa infections.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa",
pages = "2333-2330",
number = "24",
volume = "28",
doi = "10.1080/14786419.2014.934239"
}

Pejin, B., Talevska, A., Ciric, A., Glamočlija, J., Nikolic, M., Talevski, T.,& Soković, M.. (2014). Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(24), 2330-2333.
https://doi.org/10.1080/14786419.2014.934239

Pejin B, Talevska A, Ciric A, Glamočlija J, Nikolic M, Talevski T, Soković M. Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa. in Natural Product Research. 2014;28(24):2330-2333.
doi:10.1080/14786419.2014.934239 .

Pejin, Boris, Talevska, Aleksandra, Ciric, Ana, Glamočlija, Jasmina, Nikolic, Milos, Talevski, Trajce, Soković, Marina, "Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa" in Natural Product Research, 28, no. 24 (2014):2330-2333,
https://doi.org/10.1080/14786419.2014.934239 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Talevska, Aleksandra
AU  - Ciric, Ana
AU  - Glamočlija, Jasmina
AU  - Nikolic, Milos
AU  - Talevski, Trajce
AU  - Soković, Marina
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/784
AB  - The antimicrobial activity of five crude extracts (aqueous, methanol, ethyl acetate, acetone and methylene chloride) of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) was evaluated in vitro by using microdilution method against eight bacterial and eight fungal strains for the first time. The extracts were proven to be active in varying degrees against all the bacteria and fungi tested. O. rotunda methanol extract exhibited the highest antibacterial activity (minimum inhibitory concentration (MIC) 7.5-15.0 mu g/mL and minimum bactericidal concentration 15-30 mu g/mL), while its acetone extract exhibited the most promising antifungal activity (MIC 7.5-45.0 mu g/mL and minimum fungicidal concentration 15-60 mu g/mL). The extracts were more effective against the bacteria and fungi screened compared with the positive controls (streptomycin and ampicillin for bacteria and bifonazole and ketoconazole for fungi, respectively). According to the experimental data obtained, this deepwater sponge species may be considered as a gold mine of new antimicrobial substances with significant and broad-range activity.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937)
EP  - 1494
IS  - 18
SP  - 1489
VL  - 28
DO  - 10.1080/14786419.2014.911297
ER  - 

@article{
author = "Pejin, Boris and Talevska, Aleksandra and Ciric, Ana and Glamočlija, Jasmina and Nikolic, Milos and Talevski, Trajce and Soković, Marina",
year = "2014",
abstract = "The antimicrobial activity of five crude extracts (aqueous, methanol, ethyl acetate, acetone and methylene chloride) of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) was evaluated in vitro by using microdilution method against eight bacterial and eight fungal strains for the first time. The extracts were proven to be active in varying degrees against all the bacteria and fungi tested. O. rotunda methanol extract exhibited the highest antibacterial activity (minimum inhibitory concentration (MIC) 7.5-15.0 mu g/mL and minimum bactericidal concentration 15-30 mu g/mL), while its acetone extract exhibited the most promising antifungal activity (MIC 7.5-45.0 mu g/mL and minimum fungicidal concentration 15-60 mu g/mL). The extracts were more effective against the bacteria and fungi screened compared with the positive controls (streptomycin and ampicillin for bacteria and bifonazole and ketoconazole for fungi, respectively). According to the experimental data obtained, this deepwater sponge species may be considered as a gold mine of new antimicrobial substances with significant and broad-range activity.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937)",
pages = "1494-1489",
number = "18",
volume = "28",
doi = "10.1080/14786419.2014.911297"
}

Pejin, B., Talevska, A., Ciric, A., Glamočlija, J., Nikolic, M., Talevski, T.,& Soković, M.. (2014). In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937). in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(18), 1489-1494.
https://doi.org/10.1080/14786419.2014.911297

Pejin B, Talevska A, Ciric A, Glamočlija J, Nikolic M, Talevski T, Soković M. In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937). in Natural Product Research. 2014;28(18):1489-1494.
doi:10.1080/14786419.2014.911297 .

Pejin, Boris, Talevska, Aleksandra, Ciric, Ana, Glamočlija, Jasmina, Nikolic, Milos, Talevski, Trajce, Soković, Marina, "In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937)" in Natural Product Research, 28, no. 18 (2014):1489-1494,
https://doi.org/10.1080/14786419.2014.911297 . .