TY  - JOUR
AU  - Peric, Mina
AU  - Bataveljic, Danijela
AU  - Bijelic, Dunja
AU  - Milicević, Katarina
AU  - Andjus, Pavle R.
AU  - Bogdanović Pristov, Jelena
AU  - Nikolić, Ljiljana M.
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1546
AB  - We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.
PB  - Wiley, Hoboken
T2  - Microscopy Research and Technique
T1  - Approach for patch-clamping using an upright microscope with z-axis movable stage
DO  - 10.1002/jemt.24066
ER  - 

@article{
author = "Peric, Mina and Bataveljic, Danijela and Bijelic, Dunja and Milicević, Katarina and Andjus, Pavle R. and Bogdanović Pristov, Jelena and Nikolić, Ljiljana M.",
year = "2022",
abstract = "We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.",
publisher = "Wiley, Hoboken",
journal = "Microscopy Research and Technique",
title = "Approach for patch-clamping using an upright microscope with z-axis movable stage",
doi = "10.1002/jemt.24066"
}

Peric, M., Bataveljic, D., Bijelic, D., Milicević, K., Andjus, P. R., Bogdanović Pristov, J.,& Nikolić, L. M.. (2022). Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique
Wiley, Hoboken..
https://doi.org/10.1002/jemt.24066

Peric M, Bataveljic D, Bijelic D, Milicević K, Andjus PR, Bogdanović Pristov J, Nikolić LM. Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique. 2022;.
doi:10.1002/jemt.24066 .

Peric, Mina, Bataveljic, Danijela, Bijelic, Dunja, Milicević, Katarina, Andjus, Pavle R., Bogdanović Pristov, Jelena, Nikolić, Ljiljana M., "Approach for patch-clamping using an upright microscope with z-axis movable stage" in Microscopy Research and Technique (2022),
https://doi.org/10.1002/jemt.24066 . .

TY  - JOUR
AU  - Bijelic, Dunja D.
AU  - Milicević, Katarina
AU  - Lazarević, Milica N.
AU  - Miljković, Đorđe
AU  - Bogdanović Pristov, Jelena
AU  - Savić, Danijela Z
AU  - Petković, Branka
AU  - Andjus, Pavle R.
AU  - Momcilović, Miljana
AU  - Nikolić, Ljiljana M.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1317
AB  - Interaction between autoreactive immune cells and astroglia is an important part of the pathologic processes that fuel neurodegeneration in multiple sclerosis. In this inflammatory disease, immune cells enter into the central nervous system (CNS) and they spread through CNS parenchyma, but the impact of these autoreactive immune cells on the activity pattern of astrocytes has not been defined. By exploiting naive astrocytes in culture and CNS-infiltrated immune cells (CNS IICs) isolated from rat with experimental autoimmune encephalomyelitis (EAE), here we demonstrate previously unrecognized properties of immune cell-astrocyte interaction. We show that CNS IICs but not the peripheral immune cell application, evokes a rapid and vigorous intracellular Ca(2+)increase in astrocytes by promoting glial release of ATP. ATP propagated Ca(2+)elevation through glial purinergic P2X7 receptor activation by the hemichannel-dependent nucleotide release mechanism. Astrocyte Ca(2+)increase is specifically triggered by the autoreactive CD4(+)T-cell application and these two cell types exhibit close spatial interaction in EAE. Therefore, Ca(2+)signals may mediate a rapid astroglial response to the autoreactive immune cells in their local environment. This property of immune cell-astrocyte interaction may be important to consider in studies interrogating CNS autoimmune disease.
PB  - Wiley, Hoboken
T2  - Journal of Neuroscience Research
T1  - Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling
EP  - 2332
IS  - 11
SP  - 2317
VL  - 98
DO  - 10.1002/jnr.24699
ER  - 

@article{
author = "Bijelic, Dunja D. and Milicević, Katarina and Lazarević, Milica N. and Miljković, Đorđe and Bogdanović Pristov, Jelena and Savić, Danijela Z and Petković, Branka and Andjus, Pavle R. and Momcilović, Miljana and Nikolić, Ljiljana M.",
year = "2020",
abstract = "Interaction between autoreactive immune cells and astroglia is an important part of the pathologic processes that fuel neurodegeneration in multiple sclerosis. In this inflammatory disease, immune cells enter into the central nervous system (CNS) and they spread through CNS parenchyma, but the impact of these autoreactive immune cells on the activity pattern of astrocytes has not been defined. By exploiting naive astrocytes in culture and CNS-infiltrated immune cells (CNS IICs) isolated from rat with experimental autoimmune encephalomyelitis (EAE), here we demonstrate previously unrecognized properties of immune cell-astrocyte interaction. We show that CNS IICs but not the peripheral immune cell application, evokes a rapid and vigorous intracellular Ca(2+)increase in astrocytes by promoting glial release of ATP. ATP propagated Ca(2+)elevation through glial purinergic P2X7 receptor activation by the hemichannel-dependent nucleotide release mechanism. Astrocyte Ca(2+)increase is specifically triggered by the autoreactive CD4(+)T-cell application and these two cell types exhibit close spatial interaction in EAE. Therefore, Ca(2+)signals may mediate a rapid astroglial response to the autoreactive immune cells in their local environment. This property of immune cell-astrocyte interaction may be important to consider in studies interrogating CNS autoimmune disease.",
publisher = "Wiley, Hoboken",
journal = "Journal of Neuroscience Research",
title = "Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling",
pages = "2332-2317",
number = "11",
volume = "98",
doi = "10.1002/jnr.24699"
}

Bijelic, D. D., Milicević, K., Lazarević, M. N., Miljković, Đ., Bogdanović Pristov, J., Savić, D. Z., Petković, B., Andjus, P. R., Momcilović, M.,& Nikolić, L. M.. (2020). Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling. in Journal of Neuroscience Research
Wiley, Hoboken., 98(11), 2317-2332.
https://doi.org/10.1002/jnr.24699

Bijelic DD, Milicević K, Lazarević MN, Miljković Đ, Bogdanović Pristov J, Savić DZ, Petković B, Andjus PR, Momcilović M, Nikolić LM. Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling. in Journal of Neuroscience Research. 2020;98(11):2317-2332.
doi:10.1002/jnr.24699 .

Bijelic, Dunja D., Milicević, Katarina, Lazarević, Milica N., Miljković, Đorđe, Bogdanović Pristov, Jelena, Savić, Danijela Z, Petković, Branka, Andjus, Pavle R., Momcilović, Miljana, Nikolić, Ljiljana M., "Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling" in Journal of Neuroscience Research, 98, no. 11 (2020):2317-2332,
https://doi.org/10.1002/jnr.24699 . .

TY  - JOUR
AU  - Korenic, Andrej
AU  - Andjus, Pavle R.
AU  - Radenović, Lidija
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/924
AB  - Astrocytes can survive nutrient deprivation (ND) for days. However, the pro-survival strategy of astrocytes under such a metabolic challenge is still not clear. In the present study, we examined the effects of inhibition of two potential steps in energy acquisition during ND: autophagy (using chloroquine) and lipolysis (using orlistat). The inhibition of autophagy did not show significant effects on cell viability until 8-9 h of ND. From that point onwards, the number of dead cells gradually increased, reaching similar to 60% between 10 and 12 h of ND. In addition, early inhibition of autophagy made astrocytes more vulnerable to the latter ND. The inhibition of lipolysis decreased the viability of cells exposed to ND, but this appeared much later compared to the inhibition of autophagy. The application of orlistat prevented ND-related hyperpolarization of the mitochondrial membrane, and mitochondria became swollen. This study clearly shows that autophagy and lipolysis are essential for the survival of astrocytes under ND conditions, which might be related to their role as neuron-supporting cells.
PB  - Elsevier Ireland Ltd, Clare
T2  - Neuroscience Letters
T1  - The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation
EP  - 133
SP  - 128
VL  - 595
DO  - 10.1016/j.neulet.2015.04.020
ER  - 

@article{
author = "Korenic, Andrej and Andjus, Pavle R. and Radenović, Lidija and Spasojević, Ivan",
year = "2015",
abstract = "Astrocytes can survive nutrient deprivation (ND) for days. However, the pro-survival strategy of astrocytes under such a metabolic challenge is still not clear. In the present study, we examined the effects of inhibition of two potential steps in energy acquisition during ND: autophagy (using chloroquine) and lipolysis (using orlistat). The inhibition of autophagy did not show significant effects on cell viability until 8-9 h of ND. From that point onwards, the number of dead cells gradually increased, reaching similar to 60% between 10 and 12 h of ND. In addition, early inhibition of autophagy made astrocytes more vulnerable to the latter ND. The inhibition of lipolysis decreased the viability of cells exposed to ND, but this appeared much later compared to the inhibition of autophagy. The application of orlistat prevented ND-related hyperpolarization of the mitochondrial membrane, and mitochondria became swollen. This study clearly shows that autophagy and lipolysis are essential for the survival of astrocytes under ND conditions, which might be related to their role as neuron-supporting cells.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Neuroscience Letters",
title = "The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation",
pages = "133-128",
volume = "595",
doi = "10.1016/j.neulet.2015.04.020"
}

Korenic, A., Andjus, P. R., Radenović, L.,& Spasojević, I.. (2015). The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation. in Neuroscience Letters
Elsevier Ireland Ltd, Clare., 595, 128-133.
https://doi.org/10.1016/j.neulet.2015.04.020

Korenic A, Andjus PR, Radenović L, Spasojević I. The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation. in Neuroscience Letters. 2015;595:128-133.
doi:10.1016/j.neulet.2015.04.020 .

Korenic, Andrej, Andjus, Pavle R., Radenović, Lidija, Spasojević, Ivan, "The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation" in Neuroscience Letters, 595 (2015):128-133,
https://doi.org/10.1016/j.neulet.2015.04.020 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Blagojević, Duško
AU  - Stević, Zorica D
AU  - Andjus, Pavle R.
AU  - Spasić, Mihajlo
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/902
AB  - In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The effects of wild-type and mutant sod1 on smooth muscle contraction
EP  - 192
IS  - 1
SP  - 187
VL  - 67
DO  - 10.2298/ABS141006023N
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Blagojević, Duško and Stević, Zorica D and Andjus, Pavle R. and Spasić, Mihajlo",
year = "2015",
abstract = "In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The effects of wild-type and mutant sod1 on smooth muscle contraction",
pages = "192-187",
number = "1",
volume = "67",
doi = "10.2298/ABS141006023N"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Blagojević, D., Stević, Z. D., Andjus, P. R.,& Spasić, M.. (2015). The effects of wild-type and mutant sod1 on smooth muscle contraction. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(1), 187-192.
https://doi.org/10.2298/ABS141006023N

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Blagojević D, Stević ZD, Andjus PR, Spasić M. The effects of wild-type and mutant sod1 on smooth muscle contraction. in Archives of Biological Sciences. 2015;67(1):187-192.
doi:10.2298/ABS141006023N .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Blagojević, Duško, Stević, Zorica D, Andjus, Pavle R., Spasić, Mihajlo, "The effects of wild-type and mutant sod1 on smooth muscle contraction" in Archives of Biological Sciences, 67, no. 1 (2015):187-192,
https://doi.org/10.2298/ABS141006023N . .

TY  - JOUR
AU  - Bajić, Aleksandar
AU  - Spasić, Mihajlo
AU  - Andjus, Pavle R
AU  - Savić, Danijela Z
AU  - Parabucki, Ana B
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/707
AB  - The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia
IS  - 10
VL  - 8
DO  - 10.1371/journal.pone.0076383
ER  - 

@article{
author = "Bajić, Aleksandar and Spasić, Mihajlo and Andjus, Pavle R and Savić, Danijela Z and Parabucki, Ana B and Nikolić-Kokić, Aleksandra and Spasojević, Ivan",
year = "2013",
abstract = "The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia",
number = "10",
volume = "8",
doi = "10.1371/journal.pone.0076383"
}

Bajić, A., Spasić, M., Andjus, P. R., Savić, D. Z., Parabucki, A. B., Nikolić-Kokić, A.,& Spasojević, I.. (2013). Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in PLoS One
Public Library Science, San Francisco., 8(10).
https://doi.org/10.1371/journal.pone.0076383

Bajić A, Spasić M, Andjus PR, Savić DZ, Parabucki AB, Nikolić-Kokić A, Spasojević I. Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in PLoS One. 2013;8(10).
doi:10.1371/journal.pone.0076383 .

Bajić, Aleksandar, Spasić, Mihajlo, Andjus, Pavle R, Savić, Danijela Z, Parabucki, Ana B, Nikolić-Kokić, Aleksandra, Spasojević, Ivan, "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia" in PLoS One, 8, no. 10 (2013),
https://doi.org/10.1371/journal.pone.0076383 . .

TY  - JOUR
AU  - Bajić, Aleksandar
AU  - Zakrzewska, Joanna
AU  - Godjevac, Dejan M
AU  - Andjus, Pavle R
AU  - Jones, David R
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/484
AB  - The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions
EP  - 420
IS  - 3
SP  - 416
VL  - 346
DO  - 10.1016/j.carres.2010.12.008
ER  - 

@article{
author = "Bajić, Aleksandar and Zakrzewska, Joanna and Godjevac, Dejan M and Andjus, Pavle R and Jones, David R and Spasić, Mihajlo and Spasojević, Ivan",
year = "2011",
abstract = "The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions",
pages = "420-416",
number = "3",
volume = "346",
doi = "10.1016/j.carres.2010.12.008"
}

Bajić, A., Zakrzewska, J., Godjevac, D. M., Andjus, P. R., Jones, D. R., Spasić, M.,& Spasojević, I.. (2011). Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 346(3), 416-420.
https://doi.org/10.1016/j.carres.2010.12.008

Bajić A, Zakrzewska J, Godjevac DM, Andjus PR, Jones DR, Spasić M, Spasojević I. Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research. 2011;346(3):416-420.
doi:10.1016/j.carres.2010.12.008 .

Bajić, Aleksandar, Zakrzewska, Joanna, Godjevac, Dejan M, Andjus, Pavle R, Jones, David R, Spasić, Mihajlo, Spasojević, Ivan, "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions" in Carbohydrate Research, 346, no. 3 (2011):416-420,
https://doi.org/10.1016/j.carres.2010.12.008 . .

TY  - JOUR
AU  - Milošević, Milena
AU  - Višnjić Jeftić, Željka
AU  - Damjanović, Ilija
AU  - Nikčević, Miroslav
AU  - Andjus, Pavle R.
AU  - Gačić, Zoran
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/341
AB  - Photoreceptor content of fish retinas could be accessed by comparative electroretinographic (ERG) studies using flickering light stimuli that could separate rod-mediated vision where critical flicker frequency (CFE, frequency when the eye loses its ability to resolve individual light pulses) is usually less than 15 Hz from cone-mediated vision. Four fish species inhabiting different photic environments (small-spotted dogfish shark - Scyliorhinus canicula, eel - Anguilla anguilla, painted comber - Serranus scriba, Prussian carp - Carassius gibelio) were investigated. Dogfish shark b-wave amplitudes significantly decreased at low frequency of stimulation and CFF was reached at 3.2 Hz. A similar effect on the b-wave amplitude was observed in the eel, but CFF occurred at around 20 Hz. Conversely, b-waves of painted comber and Prussian carp remained unaltered under intermittent low-frequency stimulation, and CFFs were around 25 and 30 Hz, respectively. Additional support in accessing the receptor content of fish retinas was given by the characterization of the OFF-response (d-wave) after light adaptation. Monotonous time course of the b-wave dark adaptation indicated a rod dominated retina of the dogfish shark. Observed results indicate that the dogfish shark possesses preponderantly rod retina, that of the eel is rod-dominated, while Prussian carp and painted comber have cone-rich retinae.
PB  - General Physiol And Biophysics, Bratislava
T2  - General Physiology and Biophysics
T1  - Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina
EP  - 282
IS  - 3
SP  - 276
VL  - 28
DO  - 10.4149/gpb_2009_03_276
ER  - 

@article{
author = "Milošević, Milena and Višnjić Jeftić, Željka and Damjanović, Ilija and Nikčević, Miroslav and Andjus, Pavle R. and Gačić, Zoran",
year = "2009",
abstract = "Photoreceptor content of fish retinas could be accessed by comparative electroretinographic (ERG) studies using flickering light stimuli that could separate rod-mediated vision where critical flicker frequency (CFE, frequency when the eye loses its ability to resolve individual light pulses) is usually less than 15 Hz from cone-mediated vision. Four fish species inhabiting different photic environments (small-spotted dogfish shark - Scyliorhinus canicula, eel - Anguilla anguilla, painted comber - Serranus scriba, Prussian carp - Carassius gibelio) were investigated. Dogfish shark b-wave amplitudes significantly decreased at low frequency of stimulation and CFF was reached at 3.2 Hz. A similar effect on the b-wave amplitude was observed in the eel, but CFF occurred at around 20 Hz. Conversely, b-waves of painted comber and Prussian carp remained unaltered under intermittent low-frequency stimulation, and CFFs were around 25 and 30 Hz, respectively. Additional support in accessing the receptor content of fish retinas was given by the characterization of the OFF-response (d-wave) after light adaptation. Monotonous time course of the b-wave dark adaptation indicated a rod dominated retina of the dogfish shark. Observed results indicate that the dogfish shark possesses preponderantly rod retina, that of the eel is rod-dominated, while Prussian carp and painted comber have cone-rich retinae.",
publisher = "General Physiol And Biophysics, Bratislava",
journal = "General Physiology and Biophysics",
title = "Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina",
pages = "282-276",
number = "3",
volume = "28",
doi = "10.4149/gpb_2009_03_276"
}

Milošević, M., Višnjić Jeftić, Ž., Damjanović, I., Nikčević, M., Andjus, P. R.,& Gačić, Z.. (2009). Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina. in General Physiology and Biophysics
General Physiol And Biophysics, Bratislava., 28(3), 276-282.
https://doi.org/10.4149/gpb_2009_03_276

Milošević M, Višnjić Jeftić Ž, Damjanović I, Nikčević M, Andjus PR, Gačić Z. Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina. in General Physiology and Biophysics. 2009;28(3):276-282.
doi:10.4149/gpb_2009_03_276 .

Milošević, Milena, Višnjić Jeftić, Željka, Damjanović, Ilija, Nikčević, Miroslav, Andjus, Pavle R., Gačić, Zoran, "Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina" in General Physiology and Biophysics, 28, no. 3 (2009):276-282,
https://doi.org/10.4149/gpb_2009_03_276 . .

TY  - JOUR
AU  - Gačić, Zoran
AU  - Damjanović, Ilija
AU  - Bajić, Aleksandar
AU  - Milošević, Milena
AU  - Mićković, Branislav
AU  - Nikčević, Miroslav
AU  - Andjus, Pavle R.
PY  - 2007
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/220
AB  - Comparative electroretinographic studies of the d-wave evoked with long duration photo stimuli in dark- and light-adapted fish species (three marine and three freshwater) were performed. At the end of prolonged photo-stimulation in scotopic conditions a negative d-wave appears in electroretinograms of dogfish shark, eel and goldfish diminishing and eventually changing with intensity of photo-stimulation, while in rudd it only increases. Dark-adapted electroretinograms of two percids (perch and painted comber) exhibit a positive d-wave that approaches the b-wave amplitude under bright photopic conditions. Judging from the d-wave, only the rod pathway is active in dark-adapted dogfish shark, eel, and goldfish. Under the light adaptation, cone pathways are active in eel and goldfish, whereas the positive response to the end of light stimuli in dogfish shark could be explained by independent ON and OFF pathways from outer to inner retina via bipolar cells. In the case of two percids, dark adaptation has no influence on cone pathways. The d-wave of rudd behaves like cone-driven d-waves but in opposite sign. The data thus show that the d-wave form, amplitude and sign depend on interconnection of ON and OFF pathways as determined by the state of adaptation and/or type of photoreceptor.
T2  - General Physiology and Biophysics
T1  - The d-wave in fish and the state of light adaptation
EP  - 267
IS  - 4
SP  - 260
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_220
ER  - 

@article{
author = "Gačić, Zoran and Damjanović, Ilija and Bajić, Aleksandar and Milošević, Milena and Mićković, Branislav and Nikčević, Miroslav and Andjus, Pavle R.",
year = "2007",
abstract = "Comparative electroretinographic studies of the d-wave evoked with long duration photo stimuli in dark- and light-adapted fish species (three marine and three freshwater) were performed. At the end of prolonged photo-stimulation in scotopic conditions a negative d-wave appears in electroretinograms of dogfish shark, eel and goldfish diminishing and eventually changing with intensity of photo-stimulation, while in rudd it only increases. Dark-adapted electroretinograms of two percids (perch and painted comber) exhibit a positive d-wave that approaches the b-wave amplitude under bright photopic conditions. Judging from the d-wave, only the rod pathway is active in dark-adapted dogfish shark, eel, and goldfish. Under the light adaptation, cone pathways are active in eel and goldfish, whereas the positive response to the end of light stimuli in dogfish shark could be explained by independent ON and OFF pathways from outer to inner retina via bipolar cells. In the case of two percids, dark adaptation has no influence on cone pathways. The d-wave of rudd behaves like cone-driven d-waves but in opposite sign. The data thus show that the d-wave form, amplitude and sign depend on interconnection of ON and OFF pathways as determined by the state of adaptation and/or type of photoreceptor.",
journal = "General Physiology and Biophysics",
title = "The d-wave in fish and the state of light adaptation",
pages = "267-260",
number = "4",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_220"
}

Gačić, Z., Damjanović, I., Bajić, A., Milošević, M., Mićković, B., Nikčević, M.,& Andjus, P. R.. (2007). The d-wave in fish and the state of light adaptation. in General Physiology and Biophysics, 26(4), 260-267.
https://hdl.handle.net/21.15107/rcub_rimsi_220

Gačić Z, Damjanović I, Bajić A, Milošević M, Mićković B, Nikčević M, Andjus PR. The d-wave in fish and the state of light adaptation. in General Physiology and Biophysics. 2007;26(4):260-267.
https://hdl.handle.net/21.15107/rcub_rimsi_220 .

Gačić, Zoran, Damjanović, Ilija, Bajić, Aleksandar, Milošević, Milena, Mićković, Branislav, Nikčević, Miroslav, Andjus, Pavle R., "The d-wave in fish and the state of light adaptation" in General Physiology and Biophysics, 26, no. 4 (2007):260-267,
https://hdl.handle.net/21.15107/rcub_rimsi_220 .

TY  - JOUR
AU  - Andjus, RK
AU  - Damjanović, Ilija
AU  - Gačić, Zoran
AU  - Konjević, D
AU  - Andjus, Pavle R.
PY  - 1998
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/46
AB  - Although differences in visual pigments between developmental stages of the European eel are well known, the expected differences in spectral sensitivity have not been demonstrated at the electrophysiological level. In fact, one past electroretinographic study led to the conclusion that in eels there is no change in scotopic sensitivity, with increasing sexual maturity. In the present experiments, electroretinograms (ERGs) were recorded from in situ eyecups of immobilized eels Anguilla anguilla (L.) caught in coastal running waters. It was shown that the ERG b-wave is as good an indicator of spectral sensitivity as the unmasked late receptor potential (LRP) which directly reflects the responsiveness of photoreceptors. Complete spectral-sensitivity curves, based on b-wave thresholds and on thresholds of LRP subsequently isolated by means of sodium iodate, have been obtained in the same eel. Using fitted amplitude-log intensity functions for threshold calculation, and two models for computer-assisted fitting of spectral-sensitivity curves, significant differences in lambda(max) were found between yellow and silver developmental stages of the eel, identified by ocular index measurements.
PB  - Cambridge Univ Press, New York
T2  - Visual Neuroscience
T1  - Electroretinographic evaluation of spectral sensitivity in yellow and silver eels (Anguilla anguilla)
EP  - 930
IS  - 5
SP  - 923
VL  - 15
DO  - 10.1017/S0952523898155189
ER  - 

@article{
author = "Andjus, RK and Damjanović, Ilija and Gačić, Zoran and Konjević, D and Andjus, Pavle R.",
year = "1998",
abstract = "Although differences in visual pigments between developmental stages of the European eel are well known, the expected differences in spectral sensitivity have not been demonstrated at the electrophysiological level. In fact, one past electroretinographic study led to the conclusion that in eels there is no change in scotopic sensitivity, with increasing sexual maturity. In the present experiments, electroretinograms (ERGs) were recorded from in situ eyecups of immobilized eels Anguilla anguilla (L.) caught in coastal running waters. It was shown that the ERG b-wave is as good an indicator of spectral sensitivity as the unmasked late receptor potential (LRP) which directly reflects the responsiveness of photoreceptors. Complete spectral-sensitivity curves, based on b-wave thresholds and on thresholds of LRP subsequently isolated by means of sodium iodate, have been obtained in the same eel. Using fitted amplitude-log intensity functions for threshold calculation, and two models for computer-assisted fitting of spectral-sensitivity curves, significant differences in lambda(max) were found between yellow and silver developmental stages of the eel, identified by ocular index measurements.",
publisher = "Cambridge Univ Press, New York",
journal = "Visual Neuroscience",
title = "Electroretinographic evaluation of spectral sensitivity in yellow and silver eels (Anguilla anguilla)",
pages = "930-923",
number = "5",
volume = "15",
doi = "10.1017/S0952523898155189"
}

Andjus, R., Damjanović, I., Gačić, Z., Konjević, D.,& Andjus, P. R.. (1998). Electroretinographic evaluation of spectral sensitivity in yellow and silver eels (Anguilla anguilla). in Visual Neuroscience
Cambridge Univ Press, New York., 15(5), 923-930.
https://doi.org/10.1017/S0952523898155189

Andjus R, Damjanović I, Gačić Z, Konjević D, Andjus PR. Electroretinographic evaluation of spectral sensitivity in yellow and silver eels (Anguilla anguilla). in Visual Neuroscience. 1998;15(5):923-930.
doi:10.1017/S0952523898155189 .

Andjus, RK, Damjanović, Ilija, Gačić, Zoran, Konjević, D, Andjus, Pavle R., "Electroretinographic evaluation of spectral sensitivity in yellow and silver eels (Anguilla anguilla)" in Visual Neuroscience, 15, no. 5 (1998):923-930,
https://doi.org/10.1017/S0952523898155189 . .