TY  - JOUR
AU  - Bajić, Aleksandar
AU  - Zakrzewska, Joanna
AU  - Godjevac, Dejan M
AU  - Andjus, Pavle R.
AU  - Jones, David R
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/484
AB  - The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions
EP  - 420
IS  - 3
SP  - 416
VL  - 346
DO  - 10.1016/j.carres.2010.12.008
ER  - 

@article{
author = "Bajić, Aleksandar and Zakrzewska, Joanna and Godjevac, Dejan M and Andjus, Pavle R. and Jones, David R and Spasić, Mihajlo and Spasojević, Ivan",
year = "2011",
abstract = "The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions",
pages = "420-416",
number = "3",
volume = "346",
doi = "10.1016/j.carres.2010.12.008"
}

Bajić, A., Zakrzewska, J., Godjevac, D. M., Andjus, P. R., Jones, D. R., Spasić, M.,& Spasojević, I.. (2011). Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 346(3), 416-420.
https://doi.org/10.1016/j.carres.2010.12.008

Bajić A, Zakrzewska J, Godjevac DM, Andjus PR, Jones DR, Spasić M, Spasojević I. Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research. 2011;346(3):416-420.
doi:10.1016/j.carres.2010.12.008 .

Bajić, Aleksandar, Zakrzewska, Joanna, Godjevac, Dejan M, Andjus, Pavle R., Jones, David R, Spasić, Mihajlo, Spasojević, Ivan, "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions" in Carbohydrate Research, 346, no. 3 (2011):416-420,
https://doi.org/10.1016/j.carres.2010.12.008 . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Zeković, Zoran
AU  - Mujic, Ibrahim
AU  - Godevac, Dejan
AU  - Mojović, Miloš
AU  - Mujic, Aida
AU  - Spasojević, Ivan
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/360
AB  - Electron paramagnetic resonance (EPR) spin-trapping and spin-probing techniques were applied to determine antioxidant activity of extracts of catkin, leaves, and spiny burs of Castanea sativa against physiologically relevant reactive species-superoxide and hydroxyl radical generated in simple chemical systems and hydrogen peroxide applied on erythrocytes. Efflux of K+ was used as a marker of membrane integrity. Chemical composition of extracts was analyzed using HPLC/DAD and LC/MS. Extracts showed high antioxidative capacity against superoxide but lower activity against hydroxyl radical. They protected fluidity and integrity of membranes of erythrocytes exposed to hydrogen peroxide. Levels of derivatives of ellagitannins showed positive correlation with the antioxidative activity of extracts. Therefore, ellagitannins from chestnut extracts could represent easily accessible natural antioxidants and beneficial component of human diet in pathophysiological conditions related to oxidative stress. In conclusion, EPR spectroscopy represents a valuable tool for evaluation of antioxidant activity in both hydrophilic and lipophilic media.
PB  - Springer, New York
T2  - Food Biophysics
T1  - EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa
EP  - 133
IS  - 2
SP  - 126
VL  - 4
DO  - 10.1007/s11483-009-9109-8
ER  - 

@article{
author = "Živković, Jelena and Zeković, Zoran and Mujic, Ibrahim and Godevac, Dejan and Mojović, Miloš and Mujic, Aida and Spasojević, Ivan",
year = "2009",
abstract = "Electron paramagnetic resonance (EPR) spin-trapping and spin-probing techniques were applied to determine antioxidant activity of extracts of catkin, leaves, and spiny burs of Castanea sativa against physiologically relevant reactive species-superoxide and hydroxyl radical generated in simple chemical systems and hydrogen peroxide applied on erythrocytes. Efflux of K+ was used as a marker of membrane integrity. Chemical composition of extracts was analyzed using HPLC/DAD and LC/MS. Extracts showed high antioxidative capacity against superoxide but lower activity against hydroxyl radical. They protected fluidity and integrity of membranes of erythrocytes exposed to hydrogen peroxide. Levels of derivatives of ellagitannins showed positive correlation with the antioxidative activity of extracts. Therefore, ellagitannins from chestnut extracts could represent easily accessible natural antioxidants and beneficial component of human diet in pathophysiological conditions related to oxidative stress. In conclusion, EPR spectroscopy represents a valuable tool for evaluation of antioxidant activity in both hydrophilic and lipophilic media.",
publisher = "Springer, New York",
journal = "Food Biophysics",
title = "EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa",
pages = "133-126",
number = "2",
volume = "4",
doi = "10.1007/s11483-009-9109-8"
}

Živković, J., Zeković, Z., Mujic, I., Godevac, D., Mojović, M., Mujic, A.,& Spasojević, I.. (2009). EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa. in Food Biophysics
Springer, New York., 4(2), 126-133.
https://doi.org/10.1007/s11483-009-9109-8

Živković J, Zeković Z, Mujic I, Godevac D, Mojović M, Mujic A, Spasojević I. EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa. in Food Biophysics. 2009;4(2):126-133.
doi:10.1007/s11483-009-9109-8 .

Živković, Jelena, Zeković, Zoran, Mujic, Ibrahim, Godevac, Dejan, Mojović, Miloš, Mujic, Aida, Spasojević, Ivan, "EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa" in Food Biophysics, 4, no. 2 (2009):126-133,
https://doi.org/10.1007/s11483-009-9109-8 . .

TY  - JOUR
AU  - Godevac, Dejan
AU  - Vujisic, Ljubodrag V
AU  - Mojović, Miloš
AU  - Ignjatović, Aleksandar
AU  - Spasojević, Ivan
AU  - Vajs, Vlatka E
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/289
AB  - A total of 20 components were identified in Allium ursinum volatile oil (AUVO) by GC-MS, and 10 of them are found for the first time in this plant species. The antioxidant capacity of AUVO was examined by beta-carotene-linoleic acid bleaching, lipid peroxidation by Fenton reaction, EPR spin-probing assay using stable nitroxide radicals, DPPH., and ABTS(.+) scavenging assays. Reaction mechanism of the volatile oil components with nitroxide radicals, based on IR spectra analysis, is proposed. AUVO demonstrated poor scavenging ability against DPPH, and ABTS(.+) comparing to synthetic antioxidants BHT and trolox, while in beta-carotene-linoleic acid system AUVO showed an effect comparable to those for BHT. AUVO was also capable to scavenge stable nitroxide radicals such as water-soluble Tempone, and 7-DS and 12-DS, incorporated into the liposome membrane. Finally, AUVO increased membrane fluidity, which could be an important feature for further in vivo investigation of some disorders, such as hypertension and atherosclerosis.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity
EP  - 1700
IS  - 4
SP  - 1692
VL  - 107
DO  - 10.1016/j.foodchem.2007.10.017
ER  - 

@article{
author = "Godevac, Dejan and Vujisic, Ljubodrag V and Mojović, Miloš and Ignjatović, Aleksandar and Spasojević, Ivan and Vajs, Vlatka E",
year = "2008",
abstract = "A total of 20 components were identified in Allium ursinum volatile oil (AUVO) by GC-MS, and 10 of them are found for the first time in this plant species. The antioxidant capacity of AUVO was examined by beta-carotene-linoleic acid bleaching, lipid peroxidation by Fenton reaction, EPR spin-probing assay using stable nitroxide radicals, DPPH., and ABTS(.+) scavenging assays. Reaction mechanism of the volatile oil components with nitroxide radicals, based on IR spectra analysis, is proposed. AUVO demonstrated poor scavenging ability against DPPH, and ABTS(.+) comparing to synthetic antioxidants BHT and trolox, while in beta-carotene-linoleic acid system AUVO showed an effect comparable to those for BHT. AUVO was also capable to scavenge stable nitroxide radicals such as water-soluble Tempone, and 7-DS and 12-DS, incorporated into the liposome membrane. Finally, AUVO increased membrane fluidity, which could be an important feature for further in vivo investigation of some disorders, such as hypertension and atherosclerosis.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity",
pages = "1700-1692",
number = "4",
volume = "107",
doi = "10.1016/j.foodchem.2007.10.017"
}

Godevac, D., Vujisic, L. V., Mojović, M., Ignjatović, A., Spasojević, I.,& Vajs, V. E.. (2008). Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity. in Food Chemistry
Elsevier Sci Ltd, Oxford., 107(4), 1692-1700.
https://doi.org/10.1016/j.foodchem.2007.10.017

Godevac D, Vujisic LV, Mojović M, Ignjatović A, Spasojević I, Vajs VE. Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity. in Food Chemistry. 2008;107(4):1692-1700.
doi:10.1016/j.foodchem.2007.10.017 .

Godevac, Dejan, Vujisic, Ljubodrag V, Mojović, Miloš, Ignjatović, Aleksandar, Spasojević, Ivan, Vajs, Vlatka E, "Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity" in Food Chemistry, 107, no. 4 (2008):1692-1700,
https://doi.org/10.1016/j.foodchem.2007.10.017 . .

TY  - JOUR
AU  - Godevac, Dejan
AU  - Pejin, Boris
AU  - Zdunic, Gordana
AU  - Savikin, Katarina
AU  - Stesević, Danijela
AU  - Vajs, Vlatka E
AU  - Milosavljević, Slobodan
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/298
AB  - Rutin (1, main constituent) and two flavone C-glycosides, vitexin (2) and vitexin 2 ''-O-alpha-rhamnopyranoside (3) were isolated from the aerial parts of Onobrychis montana subsp. scardica. They were identified by H-1-NMR, C-13-NMR and UV-Vis spectroscopy (procedure with shift reagents), and high resolution ESI-MS. A relatively high content of 1 (5.27 mg/g of dry plant material), measured by HPLC, indicated O. montana subsp. scardica as a new natural source of this biologically active compound. The isolated flavonoid compounds might be of value as chemotaxonomic markers.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Flavonoids from the aerial parts of Onobrychis montana subsp scardica
EP  - 529
IS  - 5
SP  - 525
VL  - 73
DO  - 10.2298/JSC0805525G
ER  - 

@article{
author = "Godevac, Dejan and Pejin, Boris and Zdunic, Gordana and Savikin, Katarina and Stesević, Danijela and Vajs, Vlatka E and Milosavljević, Slobodan",
year = "2008",
abstract = "Rutin (1, main constituent) and two flavone C-glycosides, vitexin (2) and vitexin 2 ''-O-alpha-rhamnopyranoside (3) were isolated from the aerial parts of Onobrychis montana subsp. scardica. They were identified by H-1-NMR, C-13-NMR and UV-Vis spectroscopy (procedure with shift reagents), and high resolution ESI-MS. A relatively high content of 1 (5.27 mg/g of dry plant material), measured by HPLC, indicated O. montana subsp. scardica as a new natural source of this biologically active compound. The isolated flavonoid compounds might be of value as chemotaxonomic markers.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Flavonoids from the aerial parts of Onobrychis montana subsp scardica",
pages = "529-525",
number = "5",
volume = "73",
doi = "10.2298/JSC0805525G"
}

Godevac, D., Pejin, B., Zdunic, G., Savikin, K., Stesević, D., Vajs, V. E.,& Milosavljević, S.. (2008). Flavonoids from the aerial parts of Onobrychis montana subsp scardica. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 73(5), 525-529.
https://doi.org/10.2298/JSC0805525G

Godevac D, Pejin B, Zdunic G, Savikin K, Stesević D, Vajs VE, Milosavljević S. Flavonoids from the aerial parts of Onobrychis montana subsp scardica. in Journal of the Serbian Chemical Society. 2008;73(5):525-529.
doi:10.2298/JSC0805525G .

Godevac, Dejan, Pejin, Boris, Zdunic, Gordana, Savikin, Katarina, Stesević, Danijela, Vajs, Vlatka E, Milosavljević, Slobodan, "Flavonoids from the aerial parts of Onobrychis montana subsp scardica" in Journal of the Serbian Chemical Society, 73, no. 5 (2008):525-529,
https://doi.org/10.2298/JSC0805525G . .