TY  - CONF
AU  - Popović, Nikolina
AU  - Prodanović, Olivera
AU  - Gadjanski, Ivana
AU  - Cvetković, Danijela
AU  - Živanović, Marko
AU  - Pavlović, Vladimir
AU  - Filipović, Nenad D.
AU  - Prodanović, Radivoje
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3110
AB  - Carboxymethylcellulose (CMC) and alginate (ALG) are
water-soluble polysaccharides used in food and
cosmetics industry. They have big potential for use in
pharmaceutical products due to their high
biocompatibility, biodegradibility, low immunogenicity
and low price. When crosslinked they can absorb large
amounts of water and swell to form hydrogels with
great physical properties. The need for new
biomaterials and hydrogels is growing daily, due to
their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we
modified ALG and CMC, in order to get a cross-linkable
polymer that can make hydrogels by chemical and
enzymatic means. After periodate oxidation we
obtained polysaccharides with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further
modification using reductive amination in the presence
of different phenolic compounds like tyramine, was
done. This modification was confirmed by UV–VIS and
FT-IR spectroscopy, while concentration of phenol and
ionizable groups was determined using absorbance at
275 nm and acid–base titration. All CMC and AlG
tyramines were able to form hydrogels after crosslinking
with horse radish peroxidase (HRP) and
hydrogen peroxide. Both derivatives have been
successfully electrospun and crosslinked afterwards.
Due to the introduction of amino groups and decrease
in molecular weight, they were significantly more
soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also
improved. We aim to make nanofibers using tyraminepolysaccharides
that will be more stable in cell culture
media after cross-linking covalently and with
calcium/barium ions. Diameter of nanofibers was
determined by scanning electron microscopy (SEM).
Cross-linked nanofibers that we obtained will be used
for tissue engineering of blood vessels.
PB  - Serbian Society for Computational Mechanics
C3  - 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia
T1  - Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig
SP  - T.1.4
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3110
ER  - 

@conference{
author = "Popović, Nikolina and Prodanović, Olivera and Gadjanski, Ivana and Cvetković, Danijela and Živanović, Marko and Pavlović, Vladimir and Filipović, Nenad D. and Prodanović, Radivoje",
year = "2017",
abstract = "Carboxymethylcellulose (CMC) and alginate (ALG) are
water-soluble polysaccharides used in food and
cosmetics industry. They have big potential for use in
pharmaceutical products due to their high
biocompatibility, biodegradibility, low immunogenicity
and low price. When crosslinked they can absorb large
amounts of water and swell to form hydrogels with
great physical properties. The need for new
biomaterials and hydrogels is growing daily, due to
their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we
modified ALG and CMC, in order to get a cross-linkable
polymer that can make hydrogels by chemical and
enzymatic means. After periodate oxidation we
obtained polysaccharides with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further
modification using reductive amination in the presence
of different phenolic compounds like tyramine, was
done. This modification was confirmed by UV–VIS and
FT-IR spectroscopy, while concentration of phenol and
ionizable groups was determined using absorbance at
275 nm and acid–base titration. All CMC and AlG
tyramines were able to form hydrogels after crosslinking
with horse radish peroxidase (HRP) and
hydrogen peroxide. Both derivatives have been
successfully electrospun and crosslinked afterwards.
Due to the introduction of amino groups and decrease
in molecular weight, they were significantly more
soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also
improved. We aim to make nanofibers using tyraminepolysaccharides
that will be more stable in cell culture
media after cross-linking covalently and with
calcium/barium ions. Diameter of nanofibers was
determined by scanning electron microscopy (SEM).
Cross-linked nanofibers that we obtained will be used
for tissue engineering of blood vessels.",
publisher = "Serbian Society for Computational Mechanics",
journal = "4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia",
title = "Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig",
pages = "T.1.4",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3110"
}

Popović, N., Prodanović, O., Gadjanski, I., Cvetković, D., Živanović, M., Pavlović, V., Filipović, N. D.,& Prodanović, R.. (2017). Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig. in 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia
Serbian Society for Computational Mechanics., T.1.4.
https://hdl.handle.net/21.15107/rcub_rimsi_3110

Popović N, Prodanović O, Gadjanski I, Cvetković D, Živanović M, Pavlović V, Filipović ND, Prodanović R. Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig. in 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia. 2017;:T.1.4.
https://hdl.handle.net/21.15107/rcub_rimsi_3110 .

Popović, Nikolina, Prodanović, Olivera, Gadjanski, Ivana, Cvetković, Danijela, Živanović, Marko, Pavlović, Vladimir, Filipović, Nenad D., Prodanović, Radivoje, "Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig" in 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia (2017):T.1.4,
https://hdl.handle.net/21.15107/rcub_rimsi_3110 .