Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig
Аутори
Popović, NikolinaProdanović, Olivera
Gadjanski, Ivana
Cvetković, Danijela
Živanović, Marko
Pavlović, Vladimir
Filipović, Nenad D.
Prodanović, Radivoje
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Carboxymethylcellulose (CMC) and alginate (ALG) are
water-soluble polysaccharides used in food and
cosmetics industry. They have big potential for use in
pharmaceutical products due to their high
biocompatibility, biodegradibility, low immunogenicity
and low price. When crosslinked they can absorb large
amounts of water and swell to form hydrogels with
great physical properties. The need for new
biomaterials and hydrogels is growing daily, due to
their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we
modified ALG and CMC, in order to get a cross-linkable
polymer that can make hydrogels by chemical and
enzymatic means. After periodate oxidation we
obtained polysaccharides with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further
modification using reductive amination in the presence
of different phenolic compounds like tyramine, was
done. This modification was confirmed by UV–VIS and
FT-IR spectroscop...y, while concentration of phenol and
ionizable groups was determined using absorbance at
275 nm and acid–base titration. All CMC and AlG
tyramines were able to form hydrogels after crosslinking
with horse radish peroxidase (HRP) and
hydrogen peroxide. Both derivatives have been
successfully electrospun and crosslinked afterwards.
Due to the introduction of amino groups and decrease
in molecular weight, they were significantly more
soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also
improved. We aim to make nanofibers using tyraminepolysaccharides
that will be more stable in cell culture
media after cross-linking covalently and with
calcium/barium ions. Diameter of nanofibers was
determined by scanning electron microscopy (SEM).
Cross-linked nanofibers that we obtained will be used
for tissue engineering of blood vessels.
Кључне речи:
Polysaccharides / nanofibers / hydrogelsИзвор:
4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia, 2017, T.1.4-Издавач:
- Serbian Society for Computational Mechanics
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Popović, Nikolina AU - Prodanović, Olivera AU - Gadjanski, Ivana AU - Cvetković, Danijela AU - Živanović, Marko AU - Pavlović, Vladimir AU - Filipović, Nenad D. AU - Prodanović, Radivoje PY - 2017 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/3110 AB - Carboxymethylcellulose (CMC) and alginate (ALG) are water-soluble polysaccharides used in food and cosmetics industry. They have big potential for use in pharmaceutical products due to their high biocompatibility, biodegradibility, low immunogenicity and low price. When crosslinked they can absorb large amounts of water and swell to form hydrogels with great physical properties. The need for new biomaterials and hydrogels is growing daily, due to their use in tissue engineering, drug delivery and cell and enzyme immobilization studies. In this study we modified ALG and CMC, in order to get a cross-linkable polymer that can make hydrogels by chemical and enzymatic means. After periodate oxidation we obtained polysaccharides with different degrees of oxidation: 2.5, 5, 10, 15 and 20 mol%. Further modification using reductive amination in the presence of different phenolic compounds like tyramine, was done. This modification was confirmed by UV–VIS and FT-IR spectroscopy, while concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid–base titration. All CMC and AlG tyramines were able to form hydrogels after crosslinking with horse radish peroxidase (HRP) and hydrogen peroxide. Both derivatives have been successfully electrospun and crosslinked afterwards. Due to the introduction of amino groups and decrease in molecular weight, they were significantly more soluble in water up to 30 % (w/w) compared to native polysaccharides and their electrospinability also improved. We aim to make nanofibers using tyraminepolysaccharides that will be more stable in cell culture media after cross-linking covalently and with calcium/barium ions. Diameter of nanofibers was determined by scanning electron microscopy (SEM). Cross-linked nanofibers that we obtained will be used for tissue engineering of blood vessels. PB - Serbian Society for Computational Mechanics C3 - 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia T1 - Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig SP - T.1.4 UR - https://hdl.handle.net/21.15107/rcub_rimsi_3110 ER -
@conference{ author = "Popović, Nikolina and Prodanović, Olivera and Gadjanski, Ivana and Cvetković, Danijela and Živanović, Marko and Pavlović, Vladimir and Filipović, Nenad D. and Prodanović, Radivoje", year = "2017", abstract = "Carboxymethylcellulose (CMC) and alginate (ALG) are water-soluble polysaccharides used in food and cosmetics industry. They have big potential for use in pharmaceutical products due to their high biocompatibility, biodegradibility, low immunogenicity and low price. When crosslinked they can absorb large amounts of water and swell to form hydrogels with great physical properties. The need for new biomaterials and hydrogels is growing daily, due to their use in tissue engineering, drug delivery and cell and enzyme immobilization studies. In this study we modified ALG and CMC, in order to get a cross-linkable polymer that can make hydrogels by chemical and enzymatic means. After periodate oxidation we obtained polysaccharides with different degrees of oxidation: 2.5, 5, 10, 15 and 20 mol%. Further modification using reductive amination in the presence of different phenolic compounds like tyramine, was done. This modification was confirmed by UV–VIS and FT-IR spectroscopy, while concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid–base titration. All CMC and AlG tyramines were able to form hydrogels after crosslinking with horse radish peroxidase (HRP) and hydrogen peroxide. Both derivatives have been successfully electrospun and crosslinked afterwards. Due to the introduction of amino groups and decrease in molecular weight, they were significantly more soluble in water up to 30 % (w/w) compared to native polysaccharides and their electrospinability also improved. We aim to make nanofibers using tyraminepolysaccharides that will be more stable in cell culture media after cross-linking covalently and with calcium/barium ions. Diameter of nanofibers was determined by scanning electron microscopy (SEM). Cross-linked nanofibers that we obtained will be used for tissue engineering of blood vessels.", publisher = "Serbian Society for Computational Mechanics", journal = "4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia", title = "Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig", pages = "T.1.4", url = "https://hdl.handle.net/21.15107/rcub_rimsi_3110" }
Popović, N., Prodanović, O., Gadjanski, I., Cvetković, D., Živanović, M., Pavlović, V., Filipović, N. D.,& Prodanović, R.. (2017). Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig. in 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia Serbian Society for Computational Mechanics., T.1.4. https://hdl.handle.net/21.15107/rcub_rimsi_3110
Popović N, Prodanović O, Gadjanski I, Cvetković D, Živanović M, Pavlović V, Filipović ND, Prodanović R. Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig. in 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia. 2017;:T.1.4. https://hdl.handle.net/21.15107/rcub_rimsi_3110 .
Popović, Nikolina, Prodanović, Olivera, Gadjanski, Ivana, Cvetković, Danijela, Živanović, Marko, Pavlović, Vladimir, Filipović, Nenad D., Prodanović, Radivoje, "Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig" in 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia (2017):T.1.4, https://hdl.handle.net/21.15107/rcub_rimsi_3110 .