TY  - JOUR
AU  - Marković, Sanja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Bagi, Ferenc
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1542
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia
IS  - 1
VL  - 106
DO  - 10.1094/PDIS-06-21-1128-PDN
ER  - 

@article{
author = "Marković, Sanja and Milić Komić, Sonja and Jelušić, Aleksandra and Ilicic, Renata and Bagi, Ferenc and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia",
number = "1",
volume = "106",
doi = "10.1094/PDIS-06-21-1128-PDN"
}

Marković, S., Milić Komić, S., Jelušić, A., Ilicic, R., Bagi, F., Stanković, S.,& Popović, T.. (2022). First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 106(1).
https://doi.org/10.1094/PDIS-06-21-1128-PDN

Marković S, Milić Komić S, Jelušić A, Ilicic R, Bagi F, Stanković S, Popović T. First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease. 2022;106(1).
doi:10.1094/PDIS-06-21-1128-PDN .

Marković, Sanja, Milić Komić, Sonja, Jelušić, Aleksandra, Ilicic, Renata, Bagi, Ferenc, Stanković, Slaviša, Popović, Tatjana, "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia" in Plant Disease, 106, no. 1 (2022),
https://doi.org/10.1094/PDIS-06-21-1128-PDN . .

TY  - CONF
AU  - Iličić, Renata
AU  - Barać, Goran
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1949
AB  - BACKGROUND
Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated 
symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were 
frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was 
initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality.
OBJECTIVES
This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) 
in locality Maglić (Bačka) in 2021. 
METHODS 
Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves 
and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium 
bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal 
phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited 
strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to 
reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database.
RESULTS 
PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three 
(two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% 
identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited 
strains of Ca. Phytoplasma solani.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology
T1  - NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE
SP  - 916
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1949
ER  - 

@conference{
author = "Iličić, Renata and Barać, Goran and Marković, Sanja and Jelušić, Aleksandra and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated 
symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were 
frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was 
initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality.
OBJECTIVES
This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) 
in locality Maglić (Bačka) in 2021. 
METHODS 
Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves 
and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium 
bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal 
phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited 
strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to 
reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database.
RESULTS 
PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three 
(two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% 
identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited 
strains of Ca. Phytoplasma solani.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology",
title = "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE",
pages = "916",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1949"
}

Iličić, R., Barać, G., Marković, S., Jelušić, A., Stanković, S.,& Popović, T.. (2022). NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 916.
https://hdl.handle.net/21.15107/rcub_rimsi_1949

Iličić R, Barać G, Marković S, Jelušić A, Stanković S, Popović T. NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:916.
https://hdl.handle.net/21.15107/rcub_rimsi_1949 .

Iličić, Renata, Barać, Goran, Marković, Sanja, Jelušić, Aleksandra, Stanković, Slaviša, Popović, Tatjana, "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE" in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology (2022):916,
https://hdl.handle.net/21.15107/rcub_rimsi_1949 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1951
AB  - BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)
SP  - 915
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1951
ER  - 

@conference{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)",
pages = "915",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1951"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Stanković, S.,& Popović, T.. (2022). PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951

Jelušić A, Mitrović P, Marković S, Iličić R, Stanković S, Popović T. PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Stanković, Slaviša, Popović, Tatjana, "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):915,
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1914
AB  - Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.
PB  - Springer
T2  - Potato Research
T1  - Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres
EP  - 18
SP  - 1
DO  - 10.1007/s11540-022-09601-9
ER  - 

@article{
author = "Marković, Sanja and Popović, Tatjana and Berić, Tanja and Dimkić, Ivica and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2022",
abstract = "Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.",
publisher = "Springer",
journal = "Potato Research",
title = "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres",
pages = "18-1",
doi = "10.1007/s11540-022-09601-9"
}

Marković, S., Popović, T., Berić, T., Dimkić, I., Jelušić, A., Iličić, R.,& Stanković, S.. (2022). Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research
Springer., 1-18.
https://doi.org/10.1007/s11540-022-09601-9

Marković S, Popović T, Berić T, Dimkić I, Jelušić A, Iličić R, Stanković S. Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research. 2022;:1-18.
doi:10.1007/s11540-022-09601-9 .

Marković, Sanja, Popović, Tatjana, Berić, Tanja, Dimkić, Ivica, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres" in Potato Research (2022):1-18,
https://doi.org/10.1007/s11540-022-09601-9 . .

TY  - CONF
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Aćimović, Radivoje
AU  - Marković, Sanja
AU  - Iličić, Renata
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1958
AB  - The vegetable soybean “edamame” (Glicine max L.) is a 
nutritious legume having pods and seeds that can be 
harvested and consumed while they are still fresh and 
premature. Edamame is rich in micronutrients and 
vitamins and is therefore used as food and for medicinal 
purposes. In Serbia, edamame production started for the 
first time in 2019 on a 10 ha field in Bečej locality (Bačka, 
Vojvodina). Later, in 2021, its production was expanded 
to 30 ha, but in summer was followed with symptoms of 
bacterial leaf spot. The symptoms appeared on leaves in 
the form of water-soaked spots surrounded by a chlorotic 
halo that enlarged and coalesced into necrotic lesions. 
Disease incidence was from 15-20%. Isolation of the 
causal pathogen was performed by sowing of suspension 
of the macerated margins of spots/lesions from ten 
collected symptomatic leaves on nutrient agar 
supplemented with 5% sucrose. Ten representative, 
purified isolates were whitish, circular, smooth, shiny, 
levan-positive, strictly aerobic, gram-negative; positive for 
green-fluorescent pigment and tobacco hypersensitive 
response, and negative for oxidase, arginine dihydrolase, 
and potato soft rot (LOPAT group Ia). Pathogenicity of the 
isolates was confirmed on soybean cotyledons by under 
pressure atomizing a bacterial suspension (107-8 CFU mL 1
). Sequencing of genes gapA, gyrB, and rpoD showed 
100% homology of the obtained isolates with 
Pseudomonas savastanoi pv. glycinea pathotype strain 
LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, 
MOC601, R4a (gapA and gyrB), and M301765 (gapA and
rpoD) originated from soybean, all from the Plant 
Associated and Environmental Microbes Database 
(PAMDB).
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference   on Plant Pathogenic Bacteria
T1  - Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1958
ER  - 

@conference{
author = "Popović, Tatjana and Jelušić, Aleksandra and Aćimović, Radivoje and Marković, Sanja and Iličić, Renata",
year = "2022",
abstract = "The vegetable soybean “edamame” (Glicine max L.) is a 
nutritious legume having pods and seeds that can be 
harvested and consumed while they are still fresh and 
premature. Edamame is rich in micronutrients and 
vitamins and is therefore used as food and for medicinal 
purposes. In Serbia, edamame production started for the 
first time in 2019 on a 10 ha field in Bečej locality (Bačka, 
Vojvodina). Later, in 2021, its production was expanded 
to 30 ha, but in summer was followed with symptoms of 
bacterial leaf spot. The symptoms appeared on leaves in 
the form of water-soaked spots surrounded by a chlorotic 
halo that enlarged and coalesced into necrotic lesions. 
Disease incidence was from 15-20%. Isolation of the 
causal pathogen was performed by sowing of suspension 
of the macerated margins of spots/lesions from ten 
collected symptomatic leaves on nutrient agar 
supplemented with 5% sucrose. Ten representative, 
purified isolates were whitish, circular, smooth, shiny, 
levan-positive, strictly aerobic, gram-negative; positive for 
green-fluorescent pigment and tobacco hypersensitive 
response, and negative for oxidase, arginine dihydrolase, 
and potato soft rot (LOPAT group Ia). Pathogenicity of the 
isolates was confirmed on soybean cotyledons by under 
pressure atomizing a bacterial suspension (107-8 CFU mL 1
). Sequencing of genes gapA, gyrB, and rpoD showed 
100% homology of the obtained isolates with 
Pseudomonas savastanoi pv. glycinea pathotype strain 
LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, 
MOC601, R4a (gapA and gyrB), and M301765 (gapA and
rpoD) originated from soybean, all from the Plant 
Associated and Environmental Microbes Database 
(PAMDB).",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference   on Plant Pathogenic Bacteria",
title = "Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1958"
}

Popović, T., Jelušić, A., Aćimović, R., Marković, S.,& Iličić, R.. (2022). Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini., 124.
https://hdl.handle.net/21.15107/rcub_rimsi_1958

Popović T, Jelušić A, Aćimović R, Marković S, Iličić R. Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria. 2022;:124.
https://hdl.handle.net/21.15107/rcub_rimsi_1958 .

Popović, Tatjana, Jelušić, Aleksandra, Aćimović, Radivoje, Marković, Sanja, Iličić, Renata, "Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia" in 14th International Conference   on Plant Pathogenic Bacteria (2022):124,
https://hdl.handle.net/21.15107/rcub_rimsi_1958 .

TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1959
AB  - In August 2021, symptoms of soft rot appeared on cabbage 
in Futog (Bačka, Vojvodina) locality known for traditional 
cabbage cultivation in Serbia. Symptoms appeared in the 
form of sunken and soft lesions on outer head leaves, while 
interior tissue was macerated, with cream to black 
discoloration. A strong, specific odor followed the 
breakdown of the affected tissue. From the 1 ha field, a 
total of 5 heads were collected for the isolation of causing 
pathogen. Margins of healthy and dead tissues were 
macerated and plated on Crystal Violet Pectate medium. 
Five creamy-white colonies forming pits in the medium 
were purified. These bacterial isolates were positive for 
pectinolytic activity on cabbage heads and potato slices. 
They were all facultative anaerobic, Gram-negative, 
catalase-positive, and oxidase-negative; unable to produce 
diffusible fluorescent pigments. Multilocus sequence 
typing of cabbage isolates was performed by sequencing 
of four housekeeping genes (proA, dnaX, icdA, mdh). 
Based on the nucleotide BLAST (National Center for 
Biotechnology Information, NCBI) analysis of the 
sequences of genes icdA and mdh, Serbian cabbage isolates 
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene, 
identity with the same strain was 99.79% and 99.57% 
based on the proA gene. Serbian cabbage isolates also 
showed 100% identity with other NCBI deposited P. 
odoriferum strains CFBP1878 and ATCC 25272 based on 
the icdA gene, as well as ICMP 11533 based on mdh gene. 
Therefore, this first report of P. odoriferum indicates a 
wider range of Pectobacterium spp. recently described in 
Serbia.
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference   on Plant Pathogenic Bacteria
T1  - First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia
SP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1959
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Mitrović, Petar and Iličić, Renata and Jelušić, Aleksandra",
year = "2022",
abstract = "In August 2021, symptoms of soft rot appeared on cabbage 
in Futog (Bačka, Vojvodina) locality known for traditional 
cabbage cultivation in Serbia. Symptoms appeared in the 
form of sunken and soft lesions on outer head leaves, while 
interior tissue was macerated, with cream to black 
discoloration. A strong, specific odor followed the 
breakdown of the affected tissue. From the 1 ha field, a 
total of 5 heads were collected for the isolation of causing 
pathogen. Margins of healthy and dead tissues were 
macerated and plated on Crystal Violet Pectate medium. 
Five creamy-white colonies forming pits in the medium 
were purified. These bacterial isolates were positive for 
pectinolytic activity on cabbage heads and potato slices. 
They were all facultative anaerobic, Gram-negative, 
catalase-positive, and oxidase-negative; unable to produce 
diffusible fluorescent pigments. Multilocus sequence 
typing of cabbage isolates was performed by sequencing 
of four housekeeping genes (proA, dnaX, icdA, mdh). 
Based on the nucleotide BLAST (National Center for 
Biotechnology Information, NCBI) analysis of the 
sequences of genes icdA and mdh, Serbian cabbage isolates 
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene, 
identity with the same strain was 99.79% and 99.57% 
based on the proA gene. Serbian cabbage isolates also 
showed 100% identity with other NCBI deposited P. 
odoriferum strains CFBP1878 and ATCC 25272 based on 
the icdA gene, as well as ICMP 11533 based on mdh gene. 
Therefore, this first report of P. odoriferum indicates a 
wider range of Pectobacterium spp. recently described in 
Serbia.",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference   on Plant Pathogenic Bacteria",
title = "First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia",
pages = "123",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1959"
}

Marković, S., Popović, T., Mitrović, P., Iličić, R.,& Jelušić, A.. (2022). First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini., 123.
https://hdl.handle.net/21.15107/rcub_rimsi_1959

Marković S, Popović T, Mitrović P, Iličić R, Jelušić A. First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria. 2022;:123.
https://hdl.handle.net/21.15107/rcub_rimsi_1959 .

Marković, Sanja, Popović, Tatjana, Mitrović, Petar, Iličić, Renata, Jelušić, Aleksandra, "First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia" in 14th International Conference   on Plant Pathogenic Bacteria (2022):123,
https://hdl.handle.net/21.15107/rcub_rimsi_1959 .

TY  - JOUR
AU  - Milićević, Z.
AU  - Krnjajić, Slobodan
AU  - Stević, M.
AU  - Ćirković, Jovana
AU  - Jelušić, Aleksandra
AU  - Pucarević, M.
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1549
AB  - In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite-F-CSZ, nature zeolite-F-CNZ and gelatin-F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.
PB  - MDPI
T2  - Agriculture (Switzerland)
T1  - Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide
IS  - 3
VL  - 12
DO  - 10.3390/agriculture12030338
ER  - 

@article{
author = "Milićević, Z. and Krnjajić, Slobodan and Stević, M. and Ćirković, Jovana and Jelušić, Aleksandra and Pucarević, M. and Popović, Tatjana",
year = "2022",
abstract = "In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite-F-CSZ, nature zeolite-F-CNZ and gelatin-F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.",
publisher = "MDPI",
journal = "Agriculture (Switzerland)",
title = "Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide",
number = "3",
volume = "12",
doi = "10.3390/agriculture12030338"
}

Milićević, Z., Krnjajić, S., Stević, M., Ćirković, J., Jelušić, A., Pucarević, M.,& Popović, T.. (2022). Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide. in Agriculture (Switzerland)
MDPI., 12(3).
https://doi.org/10.3390/agriculture12030338

Milićević Z, Krnjajić S, Stević M, Ćirković J, Jelušić A, Pucarević M, Popović T. Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide. in Agriculture (Switzerland). 2022;12(3).
doi:10.3390/agriculture12030338 .

Milićević, Z., Krnjajić, Slobodan, Stević, M., Ćirković, Jovana, Jelušić, Aleksandra, Pucarević, M., Popović, Tatjana, "Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide" in Agriculture (Switzerland), 12, no. 3 (2022),
https://doi.org/10.3390/agriculture12030338 . .

TY  - JOUR
AU  - Ilicic, Renata
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Barac, Goran
AU  - Bagi, Ferenc
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1531
AB  - In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification
EP  - 150
IS  - 1
SP  - 140
VL  - 180
DO  - 10.1111/aab.12717
ER  - 

@article{
author = "Ilicic, Renata and Jelušić, Aleksandra and Marković, Sanja and Barac, Goran and Bagi, Ferenc and Popović, Tatjana",
year = "2022",
abstract = "In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification",
pages = "150-140",
number = "1",
volume = "180",
doi = "10.1111/aab.12717"
}

Ilicic, R., Jelušić, A., Marković, S., Barac, G., Bagi, F.,& Popović, T.. (2022). Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology
Wiley, Hoboken., 180(1), 140-150.
https://doi.org/10.1111/aab.12717

Ilicic R, Jelušić A, Marković S, Barac G, Bagi F, Popović T. Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology. 2022;180(1):140-150.
doi:10.1111/aab.12717 .

Ilicic, Renata, Jelušić, Aleksandra, Marković, Sanja, Barac, Goran, Bagi, Ferenc, Popović, Tatjana, "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification" in Annals of Applied Biology, 180, no. 1 (2022):140-150,
https://doi.org/10.1111/aab.12717 . .

TY  - CONF
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
AU  - Marković, Sanja
AU  - Blagojević, Milan
AU  - Iličić, Renata
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1957
AB  - Xanthomonas arboricola pv. pruni (Xap), prouzrokovač bakteriozne pegavosti listova i 
plodova, rak-rana i sušenja grana koštičavih voćnih vrsta, široko je rasprostranjena 
fitopatogena bakterija u svetu. Zbog šteta koje izaziva svrstana je u regulisane nekarantinske 
organizme u Evropi (EU_2019_2072_AnnexIV_RNQP), dok se u Srbiji nalazi na Listi IA deo I 
(Službeni glasnik 57/15). Parazitira vrste iz roda Prunus, a glavni domaćini od ekonomskog 
značaja su P. persica (breskva), P. persica var. nucipersica (nektarina), P. domestica (šljiva), P. 
armeniaca (kajsija) i P. dulcis (badem). U Srbiji je prvi put eksperimentalno potvrđena na 
području Fruške Gore i to na listovima breskve (Irig) tokom 2019. godine, a nakon toga 2020. 
na listovima i plodovima kajsije (Bešenovo). U ranijem periodu prisustvo Xap je potvrđeno u 
gotovo svim državama sa kojima se Srbija graniči. Cilj ovog rada je da se utvrdi genetički 
haplotip sojeva Xap izolovanih u Srbiji i uporedi sa populacijama ove vrste poreklom sa 
različitih kontinenata i biljnih domaćina. 
U radu je korišćeno 20 sojeva bakterije, 10 poreklom sa lista breskve izolovanih 2019. i 
10 poreklom sa ploda kajsije izolovanih 2020. godine. Metoda lančane reakcije polimeraze
(PCR) je korišćena za amplifikaciju dnaK, fyuA, gyrB i rpoD konzervisanih gena. 
Sekvenciranjem PCR produkata dobijene su parcijalne sekvence gena. Konkatamerna 
sekvenca (2558 nt) napravljena je spajanjem sekvenci 4 korišćena gena i korišćena za 
pravljenje haplotipske mreže (TCS algoritam implementiran u PopART v 1.7 programu) 
zajedno sa sojevima deponovanim u NCBI bazi podataka (17 sojeva Xap poreklom iz SAD, 
Novog Zelanda, Argentine, Australije, Brazila, Urugvaja, Južne Koreje, Francuske, Italije i 
Španije). 
Prema dobijenim rezultatima svi Xap sojevi poreklom iz Srbije (20) genetički su 
homogeni. Svi korišćeni Xap sojevi (37) razvrstani su u dva haplotipa; Xap sojevi poreklom iz 
Srbije kao i sojevi iz SAD, Italije, Francuske, Španije Australije i Brazila pripadaju Haplotipu I, 
dok sojevi poreklom iz Novog Zelanda, Argentine, Urugvaja i Južne Koreje pripadaju Haplotipu 
II. Rezultati ukazuju na postojanje dve genitički različite populacije ove fitopatogene bakterije. 
Takođe može se istaći da nije utvrđena korelacija u srodnosti i poreklu kao i distribuciji Xap, 
obzirom da mesto izolacije i domaćin sa koga su sojevi izolovani nisu uticali na utvrđeni 
genotip bakterije.
PB  - Društvo za zaštitu bilja Srbije
C3  - XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
T1  - DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1957
ER  - 

@conference{
author = "Popović, Tatjana and Jelušić, Aleksandra and Milovanović, Predrag and Marković, Sanja and Blagojević, Milan and Iličić, Renata",
year = "2021",
abstract = "Xanthomonas arboricola pv. pruni (Xap), prouzrokovač bakteriozne pegavosti listova i 
plodova, rak-rana i sušenja grana koštičavih voćnih vrsta, široko je rasprostranjena 
fitopatogena bakterija u svetu. Zbog šteta koje izaziva svrstana je u regulisane nekarantinske 
organizme u Evropi (EU_2019_2072_AnnexIV_RNQP), dok se u Srbiji nalazi na Listi IA deo I 
(Službeni glasnik 57/15). Parazitira vrste iz roda Prunus, a glavni domaćini od ekonomskog 
značaja su P. persica (breskva), P. persica var. nucipersica (nektarina), P. domestica (šljiva), P. 
armeniaca (kajsija) i P. dulcis (badem). U Srbiji je prvi put eksperimentalno potvrđena na 
području Fruške Gore i to na listovima breskve (Irig) tokom 2019. godine, a nakon toga 2020. 
na listovima i plodovima kajsije (Bešenovo). U ranijem periodu prisustvo Xap je potvrđeno u 
gotovo svim državama sa kojima se Srbija graniči. Cilj ovog rada je da se utvrdi genetički 
haplotip sojeva Xap izolovanih u Srbiji i uporedi sa populacijama ove vrste poreklom sa 
različitih kontinenata i biljnih domaćina. 
U radu je korišćeno 20 sojeva bakterije, 10 poreklom sa lista breskve izolovanih 2019. i 
10 poreklom sa ploda kajsije izolovanih 2020. godine. Metoda lančane reakcije polimeraze
(PCR) je korišćena za amplifikaciju dnaK, fyuA, gyrB i rpoD konzervisanih gena. 
Sekvenciranjem PCR produkata dobijene su parcijalne sekvence gena. Konkatamerna 
sekvenca (2558 nt) napravljena je spajanjem sekvenci 4 korišćena gena i korišćena za 
pravljenje haplotipske mreže (TCS algoritam implementiran u PopART v 1.7 programu) 
zajedno sa sojevima deponovanim u NCBI bazi podataka (17 sojeva Xap poreklom iz SAD, 
Novog Zelanda, Argentine, Australije, Brazila, Urugvaja, Južne Koreje, Francuske, Italije i 
Španije). 
Prema dobijenim rezultatima svi Xap sojevi poreklom iz Srbije (20) genetički su 
homogeni. Svi korišćeni Xap sojevi (37) razvrstani su u dva haplotipa; Xap sojevi poreklom iz 
Srbije kao i sojevi iz SAD, Italije, Francuske, Španije Australije i Brazila pripadaju Haplotipu I, 
dok sojevi poreklom iz Novog Zelanda, Argentine, Urugvaja i Južne Koreje pripadaju Haplotipu 
II. Rezultati ukazuju na postojanje dve genitički različite populacije ove fitopatogene bakterije. 
Takođe može se istaći da nije utvrđena korelacija u srodnosti i poreklu kao i distribuciji Xap, 
obzirom da mesto izolacije i domaćin sa koga su sojevi izolovani nisu uticali na utvrđeni 
genotip bakterije.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova",
title = "DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1957"
}

Popović, T., Jelušić, A., Milovanović, P., Marković, S., Blagojević, M.,& Iličić, R.. (2021). DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
Društvo za zaštitu bilja Srbije., 44.
https://hdl.handle.net/21.15107/rcub_rimsi_1957

Popović T, Jelušić A, Milovanović P, Marković S, Blagojević M, Iličić R. DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova. 2021;:44.
https://hdl.handle.net/21.15107/rcub_rimsi_1957 .

Popović, Tatjana, Jelušić, Aleksandra, Milovanović, Predrag, Marković, Sanja, Blagojević, Milan, Iličić, Renata, "DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI" in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova (2021):44,
https://hdl.handle.net/21.15107/rcub_rimsi_1957 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Ilicic, Renata
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1438
AB  - Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers
EP  - 1959
IS  - 8
SP  - 1945
VL  - 70
DO  - 10.1111/ppa.13421
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Ilicic, Renata and Veljović-Jovanović, Sonja and Milić Komić, Sonja and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers",
pages = "1959-1945",
number = "8",
volume = "70",
doi = "10.1111/ppa.13421"
}

Marković, S., Stanković, S., Ilicic, R., Veljović-Jovanović, S., Milić Komić, S., Jelušić, A.,& Popović, T.. (2021). Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology
Wiley, Hoboken., 70(8), 1945-1959.
https://doi.org/10.1111/ppa.13421

Marković S, Stanković S, Ilicic R, Veljović-Jovanović S, Milić Komić S, Jelušić A, Popović T. Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology. 2021;70(8):1945-1959.
doi:10.1111/ppa.13421 .

Marković, Sanja, Stanković, Slaviša, Ilicic, Renata, Veljović-Jovanović, Sonja, Milić Komić, Sonja, Jelušić, Aleksandra, Popović, Tatjana, "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers" in Plant Pathology, 70, no. 8 (2021):1945-1959,
https://doi.org/10.1111/ppa.13421 . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Marković, Sanja
AU  - Blagojević, Milan
AU  - Bagi, Ferenc
AU  - Iličić, Renata
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1980
AB  - Bakterija Pseudomonas syringae pv. morsprunorum, prouzrokovač bakteriozne 
pegavosti listova i plodova, ekonomski je značajan patogen koštičavih vrsta voćaka. Pri 
povoljim vremenskim uslovima za razvoj (kišovito i prohladno vreme) bolest se brzo širi 
zahvatajući masovno listove cele krune stabla. Pored pegavosti, P. s. pv. morsprunorum
prouzrokuje simptome uvelosti, sušenja i rak rane drvenastih delova biljnih domaćina. Cilj 
ovog rada je da se genetički okarakterišu sojevi P. syringae pv. morsprunorum poreklom sa 
obolelih listova trešnje i šljive.
U radu su korišćeni sojevi poreklom sa trešnje (lokalitet Topola, 2016. godina) i šljive 
(lokalitet Krušedol selo, 2020. godina). Genetička karakterizacija je vršena metodom analize 
multilokusnih sekvenci (MLSA), dobijenih korišćenjem 4 konzervisana gena: gapA, gltA, gyrB i 
rpoD. DNK sojeva je izolovana CTAB metodom, a amplifikacija je vršena metodom lančane 
reakcije polimeraze (PCR) prema sledećem programu: početna denaturacija na 94 °C u 
trajanju od 3 minuta, zatim 30 ciklusa denaturacije na 94 °C 2 minuta, hibridizacije na 54 °C 
(gapA), 56 °C (gltA), 62 °C (gyrB) ili 63 °C (rpoD) 1 minut i elongacije na 72 °C 1 minut, i 
finalna elongacija na 72 °C u trajanju od 10 minuta. PCR produkti su sekvencirani i dobijene 
sekvence su korišćene za konstruisanje filogenetskih stabala (MegaX program) na osnovu 
pojedinačnih i konkatamernih sekvenci. Za filogenetsku analizu su pored sojeva poreklom sa 
trešnje i šljive iz Srbije korišćeni i referentni sojevi vrsta prouzrokovača bakteriozne 
pegavosti koštičavog voća deponovani u NCBI bazi (P. s. pv. morsprunorum rase 1 i 2, P. s. pv. 
syringae, P. s. pv. avii, P. s. pv. persicae i P. cerasi).
PCR amplifikacija je rezultirala produktima veličine 634 bp (gapA), 556 bp (gltA), 610 
bp (gyrB) i 521 bp (rpoD) kod svih analiziranih sojeva. Sekvence su sređene i poravnate na 
veličine od 610 nt (gapA), 526 nt (gltA), 569 nt (gyrB) i 483 nt (rpoD). Na osnovu pojedinačnih 
gena sojevi sa trešnje i šljive iz Srbije su grupisani u jedan klaster na filogenetskom stablu, što 
ukazuje na njihovu genetičku homogenost. Zbirno filogenetsko stablo konstruisano na osnovu 
konkatamernih sekvenci sva četiri gena (2188 nt) grupisalo je sojeve sa trešnje i šljive zajedno 
sa P. s. pv. morsprunorum rasom 1 poreklom sa šljive iz Poljske i P. amygdali pv. 
morsprunorum poreklom sa trešnje iz Velike Britanije i Srbije izolovanim 2012. godine. 
Referentni sojevi ostalih Pseudomonas vrsta korišćenih za filogenetsku analizu jasno su 
odvojeni u posebne klastere. Na osnovu dobijenih rezultata može se zaključiti da su sojevi P. s.
pv. morsprunorum rasa 1 poreklom iz Srbije genetički homogeni bez obzira na godinu, 
domaćina ili lokalitet sa kog su izolovani.
PB  - Društvo za zaštitu bilja Srbije
C3  - XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
T1  - MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1980
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Marković, Sanja and Blagojević, Milan and Bagi, Ferenc and Iličić, Renata",
year = "2021",
abstract = "Bakterija Pseudomonas syringae pv. morsprunorum, prouzrokovač bakteriozne 
pegavosti listova i plodova, ekonomski je značajan patogen koštičavih vrsta voćaka. Pri 
povoljim vremenskim uslovima za razvoj (kišovito i prohladno vreme) bolest se brzo širi 
zahvatajući masovno listove cele krune stabla. Pored pegavosti, P. s. pv. morsprunorum
prouzrokuje simptome uvelosti, sušenja i rak rane drvenastih delova biljnih domaćina. Cilj 
ovog rada je da se genetički okarakterišu sojevi P. syringae pv. morsprunorum poreklom sa 
obolelih listova trešnje i šljive.
U radu su korišćeni sojevi poreklom sa trešnje (lokalitet Topola, 2016. godina) i šljive 
(lokalitet Krušedol selo, 2020. godina). Genetička karakterizacija je vršena metodom analize 
multilokusnih sekvenci (MLSA), dobijenih korišćenjem 4 konzervisana gena: gapA, gltA, gyrB i 
rpoD. DNK sojeva je izolovana CTAB metodom, a amplifikacija je vršena metodom lančane 
reakcije polimeraze (PCR) prema sledećem programu: početna denaturacija na 94 °C u 
trajanju od 3 minuta, zatim 30 ciklusa denaturacije na 94 °C 2 minuta, hibridizacije na 54 °C 
(gapA), 56 °C (gltA), 62 °C (gyrB) ili 63 °C (rpoD) 1 minut i elongacije na 72 °C 1 minut, i 
finalna elongacija na 72 °C u trajanju od 10 minuta. PCR produkti su sekvencirani i dobijene 
sekvence su korišćene za konstruisanje filogenetskih stabala (MegaX program) na osnovu 
pojedinačnih i konkatamernih sekvenci. Za filogenetsku analizu su pored sojeva poreklom sa 
trešnje i šljive iz Srbije korišćeni i referentni sojevi vrsta prouzrokovača bakteriozne 
pegavosti koštičavog voća deponovani u NCBI bazi (P. s. pv. morsprunorum rase 1 i 2, P. s. pv. 
syringae, P. s. pv. avii, P. s. pv. persicae i P. cerasi).
PCR amplifikacija je rezultirala produktima veličine 634 bp (gapA), 556 bp (gltA), 610 
bp (gyrB) i 521 bp (rpoD) kod svih analiziranih sojeva. Sekvence su sređene i poravnate na 
veličine od 610 nt (gapA), 526 nt (gltA), 569 nt (gyrB) i 483 nt (rpoD). Na osnovu pojedinačnih 
gena sojevi sa trešnje i šljive iz Srbije su grupisani u jedan klaster na filogenetskom stablu, što 
ukazuje na njihovu genetičku homogenost. Zbirno filogenetsko stablo konstruisano na osnovu 
konkatamernih sekvenci sva četiri gena (2188 nt) grupisalo je sojeve sa trešnje i šljive zajedno 
sa P. s. pv. morsprunorum rasom 1 poreklom sa šljive iz Poljske i P. amygdali pv. 
morsprunorum poreklom sa trešnje iz Velike Britanije i Srbije izolovanim 2012. godine. 
Referentni sojevi ostalih Pseudomonas vrsta korišćenih za filogenetsku analizu jasno su 
odvojeni u posebne klastere. Na osnovu dobijenih rezultata može se zaključiti da su sojevi P. s.
pv. morsprunorum rasa 1 poreklom iz Srbije genetički homogeni bez obzira na godinu, 
domaćina ili lokalitet sa kog su izolovani.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova",
title = "MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1980"
}

Jelušić, A., Popović, T., Marković, S., Blagojević, M., Bagi, F.,& Iličić, R.. (2021). MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
Društvo za zaštitu bilja Srbije., 45.
https://hdl.handle.net/21.15107/rcub_rimsi_1980

Jelušić A, Popović T, Marković S, Blagojević M, Bagi F, Iličić R. MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova. 2021;:45.
https://hdl.handle.net/21.15107/rcub_rimsi_1980 .

Jelušić, Aleksandra, Popović, Tatjana, Marković, Sanja, Blagojević, Milan, Bagi, Ferenc, Iličić, Renata, "MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI" in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova (2021):45,
https://hdl.handle.net/21.15107/rcub_rimsi_1980 .

TY  - CONF
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1961
AB  - Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, 
jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 
1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium 
atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na 
sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima 
i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši 
identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi 
distribucija populacija u 2020. i 2021. godini.
Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 
uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa 
sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 
ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti 
VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste 
kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira 
i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze 
(PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti 
su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa 
krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano 
(MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. 
Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su 
formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata 
izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama 
krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na 
mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od 
inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, 
izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. 
brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati 
prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata 
sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja 
crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je 
trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.
PB  - Društvo za zaštitu bilja Srbije
C3  - XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
T1  - NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1961
ER  - 

@conference{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović, Tatjana",
year = "2021",
abstract = "Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, 
jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 
1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium 
atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na 
sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima 
i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši 
identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi 
distribucija populacija u 2020. i 2021. godini.
Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 
uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa 
sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 
ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti 
VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste 
kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira 
i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze 
(PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti 
su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa 
krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano 
(MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. 
Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su 
formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata 
izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama 
krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na 
mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od 
inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, 
izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. 
brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati 
prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata 
sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja 
crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je 
trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova",
title = "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1961"
}

Marković, S., Stanković, S., Jelušić, A., Iličić, R.,& Popović, T.. (2021). NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
Društvo za zaštitu bilja Srbije., 17.
https://hdl.handle.net/21.15107/rcub_rimsi_1961

Marković S, Stanković S, Jelušić A, Iličić R, Popović T. NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova. 2021;:17.
https://hdl.handle.net/21.15107/rcub_rimsi_1961 .

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović, Tatjana, "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI" in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova (2021):17,
https://hdl.handle.net/21.15107/rcub_rimsi_1961 .

TY  - CONF
AU  - Oro, Violeta
AU  - Popović, Tatjana
AU  - Krnjajić, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2660
AB  - Essential oils from citrus plants are getting increasing interest in the agricultural pest
management. Numerous studies demonstrate their antibacterial, antifungal, insecticidal
and nematicidal properties. This study was conducted to determine the activity of the
essential oils from citrus plants (bergamot, bitter orange, grapefruit, mandarin, orange, and
lemon) against the rhabditid nematode Panagrolaimus sp. In vitro nematicide screening was
performed with aqueous solutions of essential oils in serial dilutions (1-0.0078%). The contact
bioassay was done in small glass Petri dishes containing 3 ml of solution and 50 nematodes
at 18oC. The experiment was carried out in five replicates. The lethal effect was monitored
after 24h. Median lethal concentration (LC50) was calculated using the Probit analysis. The
best nematicidal activity performed the essential oil from lemon, followed by bergamot.
The bitter orange had better nematicidal properties than mandarin and grapefruit essential
oils. The least activity demonstrated orange oil.
PB  - Plant Protection Society of Serbia
C3  - Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS
T1  - Nematicidal investigations of some essential oils from citrus plants
EP  - 142
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2660
ER  - 

@conference{
author = "Oro, Violeta and Popović, Tatjana and Krnjajić, Slobodan",
year = "2021",
abstract = "Essential oils from citrus plants are getting increasing interest in the agricultural pest
management. Numerous studies demonstrate their antibacterial, antifungal, insecticidal
and nematicidal properties. This study was conducted to determine the activity of the
essential oils from citrus plants (bergamot, bitter orange, grapefruit, mandarin, orange, and
lemon) against the rhabditid nematode Panagrolaimus sp. In vitro nematicide screening was
performed with aqueous solutions of essential oils in serial dilutions (1-0.0078%). The contact
bioassay was done in small glass Petri dishes containing 3 ml of solution and 50 nematodes
at 18oC. The experiment was carried out in five replicates. The lethal effect was monitored
after 24h. Median lethal concentration (LC50) was calculated using the Probit analysis. The
best nematicidal activity performed the essential oil from lemon, followed by bergamot.
The bitter orange had better nematicidal properties than mandarin and grapefruit essential
oils. The least activity demonstrated orange oil.",
publisher = "Plant Protection Society of Serbia",
journal = "Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS",
title = "Nematicidal investigations of some essential oils from citrus plants",
pages = "142-135",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2660"
}

Oro, V., Popović, T.,& Krnjajić, S.. (2021). Nematicidal investigations of some essential oils from citrus plants. in Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS
Plant Protection Society of Serbia., 135-142.
https://hdl.handle.net/21.15107/rcub_rimsi_2660

Oro V, Popović T, Krnjajić S. Nematicidal investigations of some essential oils from citrus plants. in Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS. 2021;:135-142.
https://hdl.handle.net/21.15107/rcub_rimsi_2660 .

Oro, Violeta, Popović, Tatjana, Krnjajić, Slobodan, "Nematicidal investigations of some essential oils from citrus plants" in Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS (2021):135-142,
https://hdl.handle.net/21.15107/rcub_rimsi_2660 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Kosovac, Andrea
AU  - Postic, Dobrivoj
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1494
AB  - Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Backa region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia
EP  - 1090
IS  - 4
SP  - 1080
VL  - 105
DO  - 10.1094/PDIS-05-20-1076-RE
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Ilicic, Renata and Kosovac, Andrea and Postic, Dobrivoj and Popović, Tatjana",
year = "2021",
abstract = "Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Backa region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia",
pages = "1090-1080",
number = "4",
volume = "105",
doi = "10.1094/PDIS-05-20-1076-RE"
}

Marković, S., Stanković, S., Jelušić, A., Ilicic, R., Kosovac, A., Postic, D.,& Popović, T.. (2021). Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 105(4), 1080-1090.
https://doi.org/10.1094/PDIS-05-20-1076-RE

Marković S, Stanković S, Jelušić A, Ilicic R, Kosovac A, Postic D, Popović T. Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia. in Plant Disease. 2021;105(4):1080-1090.
doi:10.1094/PDIS-05-20-1076-RE .

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Ilicic, Renata, Kosovac, Andrea, Postic, Dobrivoj, Popović, Tatjana, "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia" in Plant Disease, 105, no. 4 (2021):1080-1090,
https://doi.org/10.1094/PDIS-05-20-1076-RE . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Peeters, Kelly
AU  - Miklavcic-Visnjevec, Ana
AU  - Tavzes, Crtomir
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1426
AB  - Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates
VL  - 160
DO  - 10.1016/j.biocontrol.2021.104695
ER  - 

@article{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Peeters, Kelly and Miklavcic-Visnjevec, Ana and Tavzes, Crtomir and Stanković, Slaviša and Berić, Tanja",
year = "2021",
abstract = "Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates",
volume = "160",
doi = "10.1016/j.biocontrol.2021.104695"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Peeters, K., Miklavcic-Visnjevec, A., Tavzes, C., Stanković, S.,& Berić, T.. (2021). Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 160.
https://doi.org/10.1016/j.biocontrol.2021.104695

Jelušić A, Popović T, Dimkić I, Mitrović P, Peeters K, Miklavcic-Visnjevec A, Tavzes C, Stanković S, Berić T. Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control. 2021;160.
doi:10.1016/j.biocontrol.2021.104695 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Peeters, Kelly, Miklavcic-Visnjevec, Ana, Tavzes, Crtomir, Stanković, Slaviša, Berić, Tanja, "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates" in Biological Control, 160 (2021),
https://doi.org/10.1016/j.biocontrol.2021.104695 . .

TY  - JOUR
AU  - Ilicic, Renata
AU  - Popović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Vlajic, Slobodan
AU  - Stanković, Slaviša
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1419
AB  - Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia
EP  - 262
IS  - 1
SP  - 247
VL  - 53
DO  - 10.2298/GENSR2101247I
ER  - 

@article{
author = "Ilicic, Renata and Popović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Bagi, Ferenc and Vlajic, Slobodan and Stanković, Slaviša",
year = "2021",
abstract = "Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia",
pages = "262-247",
number = "1",
volume = "53",
doi = "10.2298/GENSR2101247I"
}

Ilicic, R., Popović, T., Marković, S., Jelušić, A., Bagi, F., Vlajic, S.,& Stanković, S.. (2021). Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 53(1), 247-262.
https://doi.org/10.2298/GENSR2101247I

Ilicic R, Popović T, Marković S, Jelušić A, Bagi F, Vlajic S, Stanković S. Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade. 2021;53(1):247-262.
doi:10.2298/GENSR2101247I .

Ilicic, Renata, Popović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Bagi, Ferenc, Vlajic, Slobodan, Stanković, Slaviša, "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia" in Genetika-Belgrade, 53, no. 1 (2021):247-262,
https://doi.org/10.2298/GENSR2101247I . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marjanović-Jeromela, Ana
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1407
AB  - Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia
EP  - 49
IS  - 1
SP  - 35
VL  - 70
DO  - 10.1111/ppa.13273
ER  - 

@article{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marjanović-Jeromela, Ana and Popović, Tatjana",
year = "2021",
abstract = "Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia",
pages = "49-35",
number = "1",
volume = "70",
doi = "10.1111/ppa.13273"
}

Jelušić, A., Berić, T., Mitrović, P., Dimkić, I., Stanković, S., Marjanović-Jeromela, A.,& Popović, T.. (2021). New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia. in Plant Pathology
Wiley, Hoboken., 70(1), 35-49.
https://doi.org/10.1111/ppa.13273

Jelušić A, Berić T, Mitrović P, Dimkić I, Stanković S, Marjanović-Jeromela A, Popović T. New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia. in Plant Pathology. 2021;70(1):35-49.
doi:10.1111/ppa.13273 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marjanović-Jeromela, Ana, Popović, Tatjana, "New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia" in Plant Pathology, 70, no. 1 (2021):35-49,
https://doi.org/10.1111/ppa.13273 . .

TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Štrbanović, Ratibor
AU  - Broćić, Zoran
AU  - Popović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Stanisavljević, Rade
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1917
AB  - The aim of this study was to determine the influence of the origin and size of planting material on the morphological 
characteristics of seed tuber's three potato cultivars. The objects of research were three varieties of potatoes: Aladdin, Newton and 
Desiree. The analysis of the morphological characteristics tuber showed highly significant (р<0.01) differences under influenced
origin planting material (factor O) and size of seed tuber (factor S). Significance influence (р<0.01) genotype (factor G) was detected 
on the number of sprouts per tuber and sprouts length. Seed tubers originating from a lower altitude (350 m a.s.l.) formed a
significantly (р<0.01) greater number of sprout per tuber, 0.59 or 7.33% more in comparison with tubers originating from 1300 m
a.s.l. Statistical analysis of the number of sprouts per tuber showed a significantly (р<0.01) lower number of germs in fractions of 40 
and 60 g, compared to the larger fraction of 80 g.
PB  - National Society of Processing and Energy in Agriculture, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS
EP  - 23
IS  - 1
SP  - 20
VL  - 25
DO  - 10.5937/jpea25-30772
ER  - 

@article{
author = "Poštić, Dobrivoj and Štrbanović, Ratibor and Broćić, Zoran and Popović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Stanisavljević, Rade",
year = "2021",
abstract = "The aim of this study was to determine the influence of the origin and size of planting material on the morphological 
characteristics of seed tuber's three potato cultivars. The objects of research were three varieties of potatoes: Aladdin, Newton and 
Desiree. The analysis of the morphological characteristics tuber showed highly significant (р<0.01) differences under influenced
origin planting material (factor O) and size of seed tuber (factor S). Significance influence (р<0.01) genotype (factor G) was detected 
on the number of sprouts per tuber and sprouts length. Seed tubers originating from a lower altitude (350 m a.s.l.) formed a
significantly (р<0.01) greater number of sprout per tuber, 0.59 or 7.33% more in comparison with tubers originating from 1300 m
a.s.l. Statistical analysis of the number of sprouts per tuber showed a significantly (р<0.01) lower number of germs in fractions of 40 
and 60 g, compared to the larger fraction of 80 g.",
publisher = "National Society of Processing and Energy in Agriculture, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS",
pages = "23-20",
number = "1",
volume = "25",
doi = "10.5937/jpea25-30772"
}

Poštić, D., Štrbanović, R., Broćić, Z., Popović, T., Marković, S., Jelušić, A.,& Stanisavljević, R.. (2021). INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS. in Journal on Processing and Energy in Agriculture
National Society of Processing and Energy in Agriculture, Novi Sad., 25(1), 20-23.
https://doi.org/10.5937/jpea25-30772

Poštić D, Štrbanović R, Broćić Z, Popović T, Marković S, Jelušić A, Stanisavljević R. INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS. in Journal on Processing and Energy in Agriculture. 2021;25(1):20-23.
doi:10.5937/jpea25-30772 .

Poštić, Dobrivoj, Štrbanović, Ratibor, Broćić, Zoran, Popović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Stanisavljević, Rade, "INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS" in Journal on Processing and Energy in Agriculture, 25, no. 1 (2021):20-23,
https://doi.org/10.5937/jpea25-30772 . .

TY  - JOUR
AU  - Mitrović, Petar
AU  - Djalović, Ivica
AU  - Kiprovski, Biljana
AU  - Veljović-Jovanović, Sonja
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1459
AB  - The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani
IS  - 2
VL  - 10
DO  - 10.3390/plants10020337
ER  - 

@article{
author = "Mitrović, Petar and Djalović, Ivica and Kiprovski, Biljana and Veljović-Jovanović, Sonja and Trkulja, Vojislav and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani",
number = "2",
volume = "10",
doi = "10.3390/plants10020337"
}

Mitrović, P., Djalović, I., Kiprovski, B., Veljović-Jovanović, S., Trkulja, V., Jelušić, A.,& Popović, T.. (2021). Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel
MDPI, Basel., 10(2).
https://doi.org/10.3390/plants10020337

Mitrović P, Djalović I, Kiprovski B, Veljović-Jovanović S, Trkulja V, Jelušić A, Popović T. Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel. 2021;10(2).
doi:10.3390/plants10020337 .

Mitrović, Petar, Djalović, Ivica, Kiprovski, Biljana, Veljović-Jovanović, Sonja, Trkulja, Vojislav, Jelušić, Aleksandra, Popović, Tatjana, "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani" in Plants-Basel, 10, no. 2 (2021),
https://doi.org/10.3390/plants10020337 . .

TY  - JOUR
AU  - Jovanović, Jelena
AU  - Krnjajić, Slobodan
AU  - Ćirković, Jovana
AU  - Radojković, Aleksandar
AU  - Popović, Tatjana
AU  - Branković, Goran
AU  - Branković, Zorica
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1353
AB  - The study reported the development of biodegradable, environment- and eco-friendly material based on natural polymers and lemongrass (Cymbopogon citratus L.) essential oil (LEO) for application as a green pesticide. The insecticidal effect of an emulsion composed of encapsulated LEO into a biopolymer matrix against the potato tuber moth (Phthorimaea operculella, Lepidoptera: Gelechiidae) (PTM) was examined and its prolonged effect compared with pure LEO. The lethal effect of the encapsulated LEO against the PTM was prolonged for up to seven days as compared to 48 h for the pure LEO. The slow release rate of the active compound (citral) from the encapsulated and the pure LEO was determined by UV-Vis spectrophotometry. After seven days the citral concentration in the encapsulated LEO was higher (60%) than in the pure LEO after 48 h (52.7%). The results of the slow release of the active compound correlated well with the insecticidal effect of the encapsulated and the pure LEO against the PTM.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella
VL  - 132
DO  - 10.1016/j.cropro.2020.105109
ER  - 

@article{
author = "Jovanović, Jelena and Krnjajić, Slobodan and Ćirković, Jovana and Radojković, Aleksandar and Popović, Tatjana and Branković, Goran and Branković, Zorica",
year = "2020",
abstract = "The study reported the development of biodegradable, environment- and eco-friendly material based on natural polymers and lemongrass (Cymbopogon citratus L.) essential oil (LEO) for application as a green pesticide. The insecticidal effect of an emulsion composed of encapsulated LEO into a biopolymer matrix against the potato tuber moth (Phthorimaea operculella, Lepidoptera: Gelechiidae) (PTM) was examined and its prolonged effect compared with pure LEO. The lethal effect of the encapsulated LEO against the PTM was prolonged for up to seven days as compared to 48 h for the pure LEO. The slow release rate of the active compound (citral) from the encapsulated and the pure LEO was determined by UV-Vis spectrophotometry. After seven days the citral concentration in the encapsulated LEO was higher (60%) than in the pure LEO after 48 h (52.7%). The results of the slow release of the active compound correlated well with the insecticidal effect of the encapsulated and the pure LEO against the PTM.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella",
volume = "132",
doi = "10.1016/j.cropro.2020.105109"
}

Jovanović, J., Krnjajić, S., Ćirković, J., Radojković, A., Popović, T., Branković, G.,& Branković, Z.. (2020). Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella. in Crop Protection
Elsevier Sci Ltd, Oxford., 132.
https://doi.org/10.1016/j.cropro.2020.105109

Jovanović J, Krnjajić S, Ćirković J, Radojković A, Popović T, Branković G, Branković Z. Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella. in Crop Protection. 2020;132.
doi:10.1016/j.cropro.2020.105109 .

Jovanović, Jelena, Krnjajić, Slobodan, Ćirković, Jovana, Radojković, Aleksandar, Popović, Tatjana, Branković, Goran, Branković, Zorica, "Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella" in Crop Protection, 132 (2020),
https://doi.org/10.1016/j.cropro.2020.105109 . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Popović, Tatjana
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1950
AB  - Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1950
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Popović, Tatjana",
year = "2020",
abstract = "Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA",
pages = "133",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1950"
}

Jelušić, A., Berić, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Popović, T.. (2020). IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950

Jelušić A, Berić T, Dimkić I, Mitrović P, Stanković S, Marković S, Popović T. IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology. 2020;:133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .

Jelušić, Aleksandra, Berić, Tanja, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Popović, Tatjana, "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA" in FEMS Online Conference on Microbiology (2020):133,
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1954
AB  - Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1954
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2020",
abstract = "Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1954"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Berić, T.. (2020). PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954

Jelušić A, Popović T, Dimkić I, Mitrović P, Stanković S, Marković S, Berić T. PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology. 2020;:134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS" in FEMS Online Conference on Microbiology (2020):134,
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .

TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1953
AB  - Background: Bacterial pathogens Ralstonia solanacearum, Clavibacter michiganensis subsp. sepedonicus and Dickeya 
dianthicola are quarantine bacteria in EPPO region and lead to severity losses in potato production. Widespread Pecto bacterium carotovorum subsp. brasiliensis also causes damages under favorable conditions. The available bactericides 
are not enough effective, therefore biological agents in their control are emerging as a possible solution. 
Objectives: The aim of this study was evaluation of antibacterial activity of Bacillus amyloliquefaciens against recently 
found bacteria in Serbian potatoes (R. solanacearum, D. dianthicola and P. c. subsp. brasiliensis).
Methods: Two antagonistic B. amyloliquefaciens strains coded as SS-12.6 and SS-38.4 were used in in vitro screening of 
antimicrobial activity against R. solanacearum (strain Rs81/18), D. dianthicola (strain Dd31) and P. c. subsp. brasiliensis 
(strain Pcb62). Pathogens and antagonists strains were grown in LB medium for 48 h at 26 C° and 30 °C, respectively. 
Supernatants and suspended pellet of full culture of SS-12.6 and SS-38.4 were tested by well diffusion assay and drop plate method, respectively. Diameter of inhibition zones were measured and expressed in mm. Biofilm formation was 
performed on microtitre plates and measured by multi-well plate readers. 
Results: Supernatants of antagonists formed inhibition zones for R. solanacearum (SS-12.6 10×10 mm; SS-38.4 11×11 
mm) and P. c. subsp. brasiliensis (SS-12.6 10×10 mm; 38.4 12×12 mm), while result for D. dianthicola was negative. 
Pellet of full antagonist cultures produced inhibition zones only for R. solanacearum (SS-12.6 10×11 mm; 38.4 11×11 
mm). The biofilm were formed in poor formation for all tested pathogens (category 1).
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS
SP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1953
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2020",
abstract = "Background: Bacterial pathogens Ralstonia solanacearum, Clavibacter michiganensis subsp. sepedonicus and Dickeya 
dianthicola are quarantine bacteria in EPPO region and lead to severity losses in potato production. Widespread Pecto bacterium carotovorum subsp. brasiliensis also causes damages under favorable conditions. The available bactericides 
are not enough effective, therefore biological agents in their control are emerging as a possible solution. 
Objectives: The aim of this study was evaluation of antibacterial activity of Bacillus amyloliquefaciens against recently 
found bacteria in Serbian potatoes (R. solanacearum, D. dianthicola and P. c. subsp. brasiliensis).
Methods: Two antagonistic B. amyloliquefaciens strains coded as SS-12.6 and SS-38.4 were used in in vitro screening of 
antimicrobial activity against R. solanacearum (strain Rs81/18), D. dianthicola (strain Dd31) and P. c. subsp. brasiliensis 
(strain Pcb62). Pathogens and antagonists strains were grown in LB medium for 48 h at 26 C° and 30 °C, respectively. 
Supernatants and suspended pellet of full culture of SS-12.6 and SS-38.4 were tested by well diffusion assay and drop plate method, respectively. Diameter of inhibition zones were measured and expressed in mm. Biofilm formation was 
performed on microtitre plates and measured by multi-well plate readers. 
Results: Supernatants of antagonists formed inhibition zones for R. solanacearum (SS-12.6 10×10 mm; SS-38.4 11×11 
mm) and P. c. subsp. brasiliensis (SS-12.6 10×10 mm; 38.4 12×12 mm), while result for D. dianthicola was negative. 
Pellet of full antagonist cultures produced inhibition zones only for R. solanacearum (SS-12.6 10×11 mm; 38.4 11×11 
mm). The biofilm were formed in poor formation for all tested pathogens (category 1).",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS",
pages = "178",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1953"
}

Marković, S., Popović, T., Jelušić, A., Iličić, R.,& Stanković, S.. (2020). POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 178.
https://hdl.handle.net/21.15107/rcub_rimsi_1953

Marković S, Popović T, Jelušić A, Iličić R, Stanković S. POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS. in FEMS Online Conference on Microbiology. 2020;:178.
https://hdl.handle.net/21.15107/rcub_rimsi_1953 .

Marković, Sanja, Popović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS" in FEMS Online Conference on Microbiology (2020):178,
https://hdl.handle.net/21.15107/rcub_rimsi_1953 .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Živković, Ljiljana
AU  - Živković, Natalija
AU  - Ilicic, Renata
AU  - Stanisavljević, Rade
AU  - Stanković, Slaviša
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1350
AB  - Fire blight disease, caused by plant pathogenic bacteriaErwinia amylovorarepresents a global threat for pome fruit industry, affecting yield and causing serious economic damage. During six year period (2011-2016), a total of 45 isolates typical forE. amylovorawere isolated from four different host plants (apple, pear, quince and medlar) in 21 localities within four Serbian regions (Southeast, Central, West, and North). Isolates were primarily identified using biochemical and serological tests, polymerase chain reaction (PCR) with species-specific primer pair (A/B) and their pathogenicity. Polyclonal antibodies used in DAS ELISA and IF were found to be specific and sensitive enough for rapidE. amylovoradetection. In virulence test performed on immature pear fruitlets, higher variability (CV) was found between regions than among hosts from which isolates originated. Slighter virulence of isolates originated from North region of Serbia (Vojvodina) was found in comparison to the isolates from Central Serbia, which were the most variable. Principal components analysis (PCA) segregated quince as a host among the most prevalent isolates in group of highly virulent, but failed to identify difference related to geographic origin of Serbian isolates. The same results enabled multivariate statistical analysis. Different DNA fingerprinting techniques such as repetitive element sequence based PCR (rep-) with BOX, ERIC, REP, GTG(5)and SERE primers, then Random Amplified Polymorphic DNA (RAPD) with M13-PCR primer revealed genetic homogeneity among all SerbianE. amylovoraisolates. ERIC, SERE and M13 primers generated the most complex and visually perceptible patters. Restriction fragment length polymorphism analysis (RFLP) ofgyrAandrecAgenes, digested with three restriction endonucleasesBstUI,BgIII andBsmI andrpoSgene digested withBstUI gave the unuque, characteristic patterns for all Serbian isolates.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates
EP  - 872
IS  - 4
SP  - 857
VL  - 157
DO  - 10.1007/s10658-020-02046-1
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Živković, Ljiljana and Živković, Natalija and Ilicic, Renata and Stanisavljević, Rade and Stanković, Slaviša",
year = "2020",
abstract = "Fire blight disease, caused by plant pathogenic bacteriaErwinia amylovorarepresents a global threat for pome fruit industry, affecting yield and causing serious economic damage. During six year period (2011-2016), a total of 45 isolates typical forE. amylovorawere isolated from four different host plants (apple, pear, quince and medlar) in 21 localities within four Serbian regions (Southeast, Central, West, and North). Isolates were primarily identified using biochemical and serological tests, polymerase chain reaction (PCR) with species-specific primer pair (A/B) and their pathogenicity. Polyclonal antibodies used in DAS ELISA and IF were found to be specific and sensitive enough for rapidE. amylovoradetection. In virulence test performed on immature pear fruitlets, higher variability (CV) was found between regions than among hosts from which isolates originated. Slighter virulence of isolates originated from North region of Serbia (Vojvodina) was found in comparison to the isolates from Central Serbia, which were the most variable. Principal components analysis (PCA) segregated quince as a host among the most prevalent isolates in group of highly virulent, but failed to identify difference related to geographic origin of Serbian isolates. The same results enabled multivariate statistical analysis. Different DNA fingerprinting techniques such as repetitive element sequence based PCR (rep-) with BOX, ERIC, REP, GTG(5)and SERE primers, then Random Amplified Polymorphic DNA (RAPD) with M13-PCR primer revealed genetic homogeneity among all SerbianE. amylovoraisolates. ERIC, SERE and M13 primers generated the most complex and visually perceptible patters. Restriction fragment length polymorphism analysis (RFLP) ofgyrAandrecAgenes, digested with three restriction endonucleasesBstUI,BgIII andBsmI andrpoSgene digested withBstUI gave the unuque, characteristic patterns for all Serbian isolates.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates",
pages = "872-857",
number = "4",
volume = "157",
doi = "10.1007/s10658-020-02046-1"
}

Popović, T., Jelušić, A., Živković, L., Živković, N., Ilicic, R., Stanisavljević, R.,& Stanković, S.. (2020). Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates. in European Journal of Plant Pathology
Springer, Dordrecht., 157(4), 857-872.
https://doi.org/10.1007/s10658-020-02046-1

Popović T, Jelušić A, Živković L, Živković N, Ilicic R, Stanisavljević R, Stanković S. Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates. in European Journal of Plant Pathology. 2020;157(4):857-872.
doi:10.1007/s10658-020-02046-1 .

Popović, Tatjana, Jelušić, Aleksandra, Živković, Ljiljana, Živković, Natalija, Ilicic, Renata, Stanisavljević, Rade, Stanković, Slaviša, "Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates" in European Journal of Plant Pathology, 157, no. 4 (2020):857-872,
https://doi.org/10.1007/s10658-020-02046-1 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Marković, Sanja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1916
AB  - Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of 
cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which aff ect 
proper cabbage growth, leading to head weight and quality losses and thereby drastically 
reducing its marketing value. The pathogen is genetically diverse, which is evident from the 
presence of eleven races worldwide and more than thirty combinations of allelic profi les. 
Therefore, this study aimed to determine the allelic profi les of Serbian cabbage Xcc strains 
obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was fi rst 
amplifi ed using polymerase chain reaction (PCR) with four housekeeping genes – P-X dnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were fi nally used 
to determine allelic profi les. Allelic profi les were determined by comparison with 33 Xcc 
strains obtained from diff erent hosts and regions, whose allelic profi les had been determined 
previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profi le 
determination and Phyloviz software for constructing a minimum spanning tree. The obtained 
allelic profi le of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and 
rpoD genes, respectively. This profi le is assigned as sequence type 2 (ST2) and it coincides 
with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No 
connection between sequence type (ST) and the host was detected.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage
EP  - 26
IS  - 1
SP  - 19
VL  - 35
DO  - 10.2298/PIF2001019P
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Marković, Sanja",
year = "2020",
abstract = "Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of 
cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which aff ect 
proper cabbage growth, leading to head weight and quality losses and thereby drastically 
reducing its marketing value. The pathogen is genetically diverse, which is evident from the 
presence of eleven races worldwide and more than thirty combinations of allelic profi les. 
Therefore, this study aimed to determine the allelic profi les of Serbian cabbage Xcc strains 
obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was fi rst 
amplifi ed using polymerase chain reaction (PCR) with four housekeeping genes – P-X dnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were fi nally used 
to determine allelic profi les. Allelic profi les were determined by comparison with 33 Xcc 
strains obtained from diff erent hosts and regions, whose allelic profi les had been determined 
previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profi le 
determination and Phyloviz software for constructing a minimum spanning tree. The obtained 
allelic profi le of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and 
rpoD genes, respectively. This profi le is assigned as sequence type 2 (ST2) and it coincides 
with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No 
connection between sequence type (ST) and the host was detected.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage",
pages = "26-19",
number = "1",
volume = "35",
doi = "10.2298/PIF2001019P"
}

Popović, T., Jelušić, A., Mitrović, P., Iličić, R.,& Marković, S.. (2020). Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 35(1), 19-26.
https://doi.org/10.2298/PIF2001019P

Popović T, Jelušić A, Mitrović P, Iličić R, Marković S. Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage. in Pesticidi i fitomedicina. 2020;35(1):19-26.
doi:10.2298/PIF2001019P .

Popović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Marković, Sanja, "Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage" in Pesticidi i fitomedicina, 35, no. 1 (2020):19-26,
https://doi.org/10.2298/PIF2001019P . .