Poređenje četiri metode za imobilizaciju HRP u alginatu radi potencijalne primene u prečišćavanju otpadnih voda

Abstract

Industrial wastewater often contains toxic phenolic compounds, which have been classified as hazardous pollutants. It has been shown that horseradish peroxidase (HRP) is able to react with phenols in water and form non-soluble compounds that could be easily removed from the aqueous phase. The aim of our work was to compare the four methods for the immobilization of HRP on alginate matrix and to find the immobilisate with the best properties. First, we immobilized HRP on the native and periodate oxidized alginate. Then we modified both the enzyme and the polymer and tried immobilizations of aminated HRP on the oxidized alginate and oxidized HRP on the aminated alginate. Imobilizate of aminated enzyme on oxidized matrix showed the best characteristics and potential for application in the continuous removal of phenol from wastewater.