Poređenje četiri metode za imobilizaciju HRP u alginatu radi potencijalne primene u prečišćavanju otpadnih voda
Authors
Spasojević, DragicaProkopijević, Miloš
Prodanović, Olivera
Radotić, Ksenija
Prodanović, Radivoje
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Industrial wastewater often contains toxic phenolic compounds, which have been classified as hazardous
pollutants. It has been shown that horseradish peroxidase (HRP) is able to react with phenols in water and
form non-soluble compounds that could be easily removed from the aqueous phase. The aim of our work was to
compare the four methods for the immobilization of HRP on alginate matrix and to find the immobilisate with
the best properties. First, we immobilized HRP on the native and periodate oxidized alginate. Then we modified
both the enzyme and the polymer and tried immobilizations of aminated HRP on the oxidized alginate and
oxidized HRP on the aminated alginate. Imobilizate of aminated enzyme on oxidized matrix showed the best
characteristics and potential for application in the continuous removal of phenol from wastewater.
Keywords:
peroksidaza iz rena / alginat / fenoli / imobilizacijaSource:
50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15. juni 2012, 2012, 199-202Publisher:
- Srpsko hemijsko društvo
Funding / projects:
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-MESTD-Basic Research (BR or ON)-173017)
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Spasojević, Dragica AU - Prokopijević, Miloš AU - Prodanović, Olivera AU - Radotić, Ksenija AU - Prodanović, Radivoje PY - 2012 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/3121 AB - Industrial wastewater often contains toxic phenolic compounds, which have been classified as hazardous pollutants. It has been shown that horseradish peroxidase (HRP) is able to react with phenols in water and form non-soluble compounds that could be easily removed from the aqueous phase. The aim of our work was to compare the four methods for the immobilization of HRP on alginate matrix and to find the immobilisate with the best properties. First, we immobilized HRP on the native and periodate oxidized alginate. Then we modified both the enzyme and the polymer and tried immobilizations of aminated HRP on the oxidized alginate and oxidized HRP on the aminated alginate. Imobilizate of aminated enzyme on oxidized matrix showed the best characteristics and potential for application in the continuous removal of phenol from wastewater. PB - Srpsko hemijsko društvo C3 - 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15. juni 2012 T1 - Poređenje četiri metode za imobilizaciju HRP u alginatu radi potencijalne primene u prečišćavanju otpadnih voda EP - 202 SP - 199 UR - https://hdl.handle.net/21.15107/rcub_rimsi_3121 ER -
@conference{ author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Radotić, Ksenija and Prodanović, Radivoje", year = "2012", abstract = "Industrial wastewater often contains toxic phenolic compounds, which have been classified as hazardous pollutants. It has been shown that horseradish peroxidase (HRP) is able to react with phenols in water and form non-soluble compounds that could be easily removed from the aqueous phase. The aim of our work was to compare the four methods for the immobilization of HRP on alginate matrix and to find the immobilisate with the best properties. First, we immobilized HRP on the native and periodate oxidized alginate. Then we modified both the enzyme and the polymer and tried immobilizations of aminated HRP on the oxidized alginate and oxidized HRP on the aminated alginate. Imobilizate of aminated enzyme on oxidized matrix showed the best characteristics and potential for application in the continuous removal of phenol from wastewater.", publisher = "Srpsko hemijsko društvo", journal = "50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15. juni 2012", title = "Poređenje četiri metode za imobilizaciju HRP u alginatu radi potencijalne primene u prečišćavanju otpadnih voda", pages = "202-199", url = "https://hdl.handle.net/21.15107/rcub_rimsi_3121" }
Spasojević, D., Prokopijević, M., Prodanović, O., Radotić, K.,& Prodanović, R.. (2012). Poređenje četiri metode za imobilizaciju HRP u alginatu radi potencijalne primene u prečišćavanju otpadnih voda. in 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15. juni 2012 Srpsko hemijsko društvo., 199-202. https://hdl.handle.net/21.15107/rcub_rimsi_3121
Spasojević D, Prokopijević M, Prodanović O, Radotić K, Prodanović R. Poređenje četiri metode za imobilizaciju HRP u alginatu radi potencijalne primene u prečišćavanju otpadnih voda. in 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15. juni 2012. 2012;:199-202. https://hdl.handle.net/21.15107/rcub_rimsi_3121 .
Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Radotić, Ksenija, Prodanović, Radivoje, "Poređenje četiri metode za imobilizaciju HRP u alginatu radi potencijalne primene u prečišćavanju otpadnih voda" in 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15. juni 2012 (2012):199-202, https://hdl.handle.net/21.15107/rcub_rimsi_3121 .