Redox interactions of epinephrine with iron at physiological pH
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Korać Jačić, JelenaStanković, Dalibor
Bogdanović Pristov, Jelena
Nikolic, Ljiljana
Spasojević, Ivan
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Epinephrine ((R)-4-(1-hydroxy-2-(methylamino)ethyl)-benzene-1,2-diol (Epi) is catecholamine that is released by the sympathetic nervous system and adrenal medulla. It is a physiologically important molecule that acts as a hormone, neurotransmitter, and medication with a broad range of effects 1-3 . Coordinate and redox interaction of Epi with iron affects the interactions with other molecules and its biological effects 4 . In this study, we reported details of redox interactions of Epi with Fe 2+ at pH 7.4, which correspond to the pH value of human plasma Epi and Fe 2+ form a complex that acts as a strong reducing agent. Cyclic voltammetry showed that the positions of E pa and E pc potentials were at approximately -480 and -1100 mV. This implies that Epi and Fe 2+ build a complex with unique redox properties. E1/2 was significantly lower compared to E0' for O 2 /O 2•- (-350 mV). It is important to point out this because superoxide radical anion is produced via spontaneous Fe 2+ reactio...n with O 2. In other words, Epi-Fe 2+ complex should be capable of reducing transition metals in (patho)physiologicaly relevant complexes that are not susceptible to reduction by O 2. Our results confirmed that Epi-Fe 2+ is capable of reducing the S-S group of glutathione disulfide. On the other hand, Epi acted in a catalyst-like fashion to promote Fe 2+ oxidation by molecular oxygen, and to a facilitated formation of the Epi–Fe 3+ complexes, at physiological pH. In addition, we examined the effects of epinepfrine and Epi/Fe3+ system on glioma cells. Epinephrine alone evokes changes in the membrane currents of glioma cells, but such effects were not observed for the complex with Fe 3+ . This implies that Epi-Fe 3+ might modulate neural activity of Epi in CNS.
Keywords:
redox iron epinephrineSource:
Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia, 2018, 141-142Publisher:
- Biohemijsko drustvo Srbije
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Korać Jačić, Jelena AU - Stanković, Dalibor AU - Bogdanović Pristov, Jelena AU - Nikolic, Ljiljana AU - Spasojević, Ivan PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2844 AB - Epinephrine ((R)-4-(1-hydroxy-2-(methylamino)ethyl)-benzene-1,2-diol (Epi) is catecholamine that is released by the sympathetic nervous system and adrenal medulla. It is a physiologically important molecule that acts as a hormone, neurotransmitter, and medication with a broad range of effects 1-3 . Coordinate and redox interaction of Epi with iron affects the interactions with other molecules and its biological effects 4 . In this study, we reported details of redox interactions of Epi with Fe 2+ at pH 7.4, which correspond to the pH value of human plasma Epi and Fe 2+ form a complex that acts as a strong reducing agent. Cyclic voltammetry showed that the positions of E pa and E pc potentials were at approximately -480 and -1100 mV. This implies that Epi and Fe 2+ build a complex with unique redox properties. E1/2 was significantly lower compared to E0' for O 2 /O 2•- (-350 mV). It is important to point out this because superoxide radical anion is produced via spontaneous Fe 2+ reaction with O 2. In other words, Epi-Fe 2+ complex should be capable of reducing transition metals in (patho)physiologicaly relevant complexes that are not susceptible to reduction by O 2. Our results confirmed that Epi-Fe 2+ is capable of reducing the S-S group of glutathione disulfide. On the other hand, Epi acted in a catalyst-like fashion to promote Fe 2+ oxidation by molecular oxygen, and to a facilitated formation of the Epi–Fe 3+ complexes, at physiological pH. In addition, we examined the effects of epinepfrine and Epi/Fe3+ system on glioma cells. Epinephrine alone evokes changes in the membrane currents of glioma cells, but such effects were not observed for the complex with Fe 3+ . This implies that Epi-Fe 3+ might modulate neural activity of Epi in CNS. PB - Biohemijsko drustvo Srbije C3 - Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia T1 - Redox interactions of epinephrine with iron at physiological pH EP - 142 SP - 141 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2844 ER -
@conference{ author = "Korać Jačić, Jelena and Stanković, Dalibor and Bogdanović Pristov, Jelena and Nikolic, Ljiljana and Spasojević, Ivan", year = "2018", abstract = "Epinephrine ((R)-4-(1-hydroxy-2-(methylamino)ethyl)-benzene-1,2-diol (Epi) is catecholamine that is released by the sympathetic nervous system and adrenal medulla. It is a physiologically important molecule that acts as a hormone, neurotransmitter, and medication with a broad range of effects 1-3 . Coordinate and redox interaction of Epi with iron affects the interactions with other molecules and its biological effects 4 . In this study, we reported details of redox interactions of Epi with Fe 2+ at pH 7.4, which correspond to the pH value of human plasma Epi and Fe 2+ form a complex that acts as a strong reducing agent. Cyclic voltammetry showed that the positions of E pa and E pc potentials were at approximately -480 and -1100 mV. This implies that Epi and Fe 2+ build a complex with unique redox properties. E1/2 was significantly lower compared to E0' for O 2 /O 2•- (-350 mV). It is important to point out this because superoxide radical anion is produced via spontaneous Fe 2+ reaction with O 2. In other words, Epi-Fe 2+ complex should be capable of reducing transition metals in (patho)physiologicaly relevant complexes that are not susceptible to reduction by O 2. Our results confirmed that Epi-Fe 2+ is capable of reducing the S-S group of glutathione disulfide. On the other hand, Epi acted in a catalyst-like fashion to promote Fe 2+ oxidation by molecular oxygen, and to a facilitated formation of the Epi–Fe 3+ complexes, at physiological pH. In addition, we examined the effects of epinepfrine and Epi/Fe3+ system on glioma cells. Epinephrine alone evokes changes in the membrane currents of glioma cells, but such effects were not observed for the complex with Fe 3+ . This implies that Epi-Fe 3+ might modulate neural activity of Epi in CNS.", publisher = "Biohemijsko drustvo Srbije", journal = "Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia", title = "Redox interactions of epinephrine with iron at physiological pH", pages = "142-141", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2844" }
Korać Jačić, J., Stanković, D., Bogdanović Pristov, J., Nikolic, L.,& Spasojević, I.. (2018). Redox interactions of epinephrine with iron at physiological pH. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia Biohemijsko drustvo Srbije., 141-142. https://hdl.handle.net/21.15107/rcub_rimsi_2844
Korać Jačić J, Stanković D, Bogdanović Pristov J, Nikolic L, Spasojević I. Redox interactions of epinephrine with iron at physiological pH. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia. 2018;:141-142. https://hdl.handle.net/21.15107/rcub_rimsi_2844 .
Korać Jačić, Jelena, Stanković, Dalibor, Bogdanović Pristov, Jelena, Nikolic, Ljiljana, Spasojević, Ivan, "Redox interactions of epinephrine with iron at physiological pH" in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia (2018):141-142, https://hdl.handle.net/21.15107/rcub_rimsi_2844 .