Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]
Autori
Bogdanović Pristov, JelenaProdanović, Radivoje
Milosavić, Nenad
Prodanović, Olivera
Radotić, Ksenija
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Fe- isoform was not present in a detectable amount.
Ključne reči:
Antioxidative response / enzyme activity / free radicals / isoelectrofocusingIzvor:
Archives of Biological Sciences, 2006, 58, 4, 211-214Izdavač:
- Serbian Biological Society
Finansiranje / projekti:
- Ispitivanja novih biosenzora za monitoring i dijagnostiku biljaka (RS-MESTD-MPN2006-2010-143043)
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Bogdanović Pristov, Jelena AU - Prodanović, Radivoje AU - Milosavić, Nenad AU - Prodanović, Olivera AU - Radotić, Ksenija PY - 2006 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2527 AB - Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Fe- isoform was not present in a detectable amount. PB - Serbian Biological Society T2 - Archives of Biological Sciences T1 - Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně] EP - 214 IS - 4 SP - 211 VL - 58 DO - 10.2298/ABS0604211B ER -
@article{ author = "Bogdanović Pristov, Jelena and Prodanović, Radivoje and Milosavić, Nenad and Prodanović, Olivera and Radotić, Ksenija", year = "2006", abstract = "Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Fe- isoform was not present in a detectable amount.", publisher = "Serbian Biological Society", journal = "Archives of Biological Sciences", title = "Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]", pages = "214-211", number = "4", volume = "58", doi = "10.2298/ABS0604211B" }
Bogdanović Pristov, J., Prodanović, R., Milosavić, N., Prodanović, O.,& Radotić, K.. (2006). Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]. in Archives of Biological Sciences Serbian Biological Society., 58(4), 211-214. https://doi.org/10.2298/ABS0604211B
Bogdanović Pristov J, Prodanović R, Milosavić N, Prodanović O, Radotić K. Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]. in Archives of Biological Sciences. 2006;58(4):211-214. doi:10.2298/ABS0604211B .
Bogdanović Pristov, Jelena, Prodanović, Radivoje, Milosavić, Nenad, Prodanović, Olivera, Radotić, Ksenija, "Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]" in Archives of Biological Sciences, 58, no. 4 (2006):211-214, https://doi.org/10.2298/ABS0604211B . .