dc.creator | Danilović Luković, Jelena | |
dc.date.accessioned | 2023-11-29T09:45:25Z | |
dc.date.available | 2023-11-29T09:45:25Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0567-8315 | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/2470 | |
dc.description.abstract | Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle
assembly and premature follicular atresia with ovary failure. This work investigates the
infl uence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on
the number and morphology of oogonia/oocytes in newborn rat pups with light and
transmission electron microscopy. Expression of apoptosis and autophagy markers in
oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn
pups had a decreased number of mitotic and resting oogonia, while the number of
altered oogonia/oocytes was increased. Ultrastructural observations revealed the
increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes
undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group.
The most abundant altered oogonia/oocytes in the hypothyroid group were those with
morphological features of advanced autophagy and Z-cells. The percentage of TUNEL
(terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/
oocytes was signifi cantly lower in the hypothyroid group. No signifi cant difference was
recorded in the expression of caspase-3, ATG7 and LC3 possibly refl ecting that these
proteins were not involved in the oogonia/oocyte alteration process during prenatal
rat hypothyroidism. The obtained results indicate that developmental hypothyroidism
in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the
advanced process of autophagy. | sr |
dc.language.iso | en | sr |
dc.publisher | Faculty of Veterinary Medicine, University of Belgrade, Serbia | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175061/RS | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Acta Veterinaria-Beograd | sr |
dc.subject | ATG7 | sr |
dc.subject | caspase-3 | sr |
dc.subject | LC3 | sr |
dc.subject | meiosis prophase I | sr |
dc.subject | secondary necrosis | sr |
dc.title | Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats | sr |
dc.type | article | sr |
dc.rights.license | BY | sr |
dc.citation.epage | 106 | |
dc.citation.issue | 1 | |
dc.citation.rank | M22 | |
dc.citation.spage | 92 | |
dc.citation.volume | 67 | |
dc.identifier.doi | 10.1515/acve-2017-0009 | |
dc.identifier.fulltext | http://rimsi.imsi.bg.ac.rs/bitstream/id/6458/bitstream_6458.pdf | |
dc.type.version | publishedVersion | sr |