ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING
Authors
Stanković, MiraProkopijević, Miloš
Bartolić, Dragana
Stevanovic, Jevrosima
Andrić, Filip
Radotić, Ksenija
Lecture (Published version)
Metadata
Show full item recordAbstract
Honey bees have a very important role in pollinating plants, in addition to honey production. Bee
diseases are one of the reasons of a significant decrease in bee colonies in the world in recent years. Honey samples originating from hives with different infestations with N. ceranae were analyzed. Total phenolic content and total protein content were determined by the Folin-Ciocalteu assay and the Bradford assay, respectively. Fluorescence spectroscopy combined with PARAFAC was used to determine the spectral components originating from proteins (PFSC1) and phenolics (PFSC2) in honey samples, and their ratios were calculated. Phenols and proteins content in the honey samples, obtained by spectrophotometric quantification, decreases with increasing infestation levels in the respective hives infected with N. ceranae. A negative correlation was obtained for the ratio of PARAFAC components PFSC2/PFSC1 and the level of infestation with N. ceranae in honey samples. These results indicate that... fluorescence spectroscopy combined with PARAFAC could be used as an optical tool for non-invasive and rapid screening of honey to estimate bee health status.
Keywords:
Apis mellifera / Nosema ceranae / fluorescence spectroscopy / proteins / TPCSource:
EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research, 2023, 40-46Publisher:
- University of Belgrade, Technical Faculty in Bor
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-MESTD-inst-2020-200143)
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - GEN AU - Stanković, Mira AU - Prokopijević, Miloš AU - Bartolić, Dragana AU - Stevanovic, Jevrosima AU - Andrić, Filip AU - Radotić, Ksenija PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2366 AB - Honey bees have a very important role in pollinating plants, in addition to honey production. Bee diseases are one of the reasons of a significant decrease in bee colonies in the world in recent years. Honey samples originating from hives with different infestations with N. ceranae were analyzed. Total phenolic content and total protein content were determined by the Folin-Ciocalteu assay and the Bradford assay, respectively. Fluorescence spectroscopy combined with PARAFAC was used to determine the spectral components originating from proteins (PFSC1) and phenolics (PFSC2) in honey samples, and their ratios were calculated. Phenols and proteins content in the honey samples, obtained by spectrophotometric quantification, decreases with increasing infestation levels in the respective hives infected with N. ceranae. A negative correlation was obtained for the ratio of PARAFAC components PFSC2/PFSC1 and the level of infestation with N. ceranae in honey samples. These results indicate that fluorescence spectroscopy combined with PARAFAC could be used as an optical tool for non-invasive and rapid screening of honey to estimate bee health status. PB - University of Belgrade, Technical Faculty in Bor T2 - EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research T1 - ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING EP - 46 SP - 40 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2366 ER -
@misc{ author = "Stanković, Mira and Prokopijević, Miloš and Bartolić, Dragana and Stevanovic, Jevrosima and Andrić, Filip and Radotić, Ksenija", year = "2023", abstract = "Honey bees have a very important role in pollinating plants, in addition to honey production. Bee diseases are one of the reasons of a significant decrease in bee colonies in the world in recent years. Honey samples originating from hives with different infestations with N. ceranae were analyzed. Total phenolic content and total protein content were determined by the Folin-Ciocalteu assay and the Bradford assay, respectively. Fluorescence spectroscopy combined with PARAFAC was used to determine the spectral components originating from proteins (PFSC1) and phenolics (PFSC2) in honey samples, and their ratios were calculated. Phenols and proteins content in the honey samples, obtained by spectrophotometric quantification, decreases with increasing infestation levels in the respective hives infected with N. ceranae. A negative correlation was obtained for the ratio of PARAFAC components PFSC2/PFSC1 and the level of infestation with N. ceranae in honey samples. These results indicate that fluorescence spectroscopy combined with PARAFAC could be used as an optical tool for non-invasive and rapid screening of honey to estimate bee health status.", publisher = "University of Belgrade, Technical Faculty in Bor", journal = "EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research", title = "ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING", pages = "46-40", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2366" }
Stanković, M., Prokopijević, M., Bartolić, D., Stevanovic, J., Andrić, F.,& Radotić, K.. (2023). ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research University of Belgrade, Technical Faculty in Bor., 40-46. https://hdl.handle.net/21.15107/rcub_rimsi_2366
Stanković M, Prokopijević M, Bartolić D, Stevanovic J, Andrić F, Radotić K. ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research. 2023;:40-46. https://hdl.handle.net/21.15107/rcub_rimsi_2366 .
Stanković, Mira, Prokopijević, Miloš, Bartolić, Dragana, Stevanovic, Jevrosima, Andrić, Filip, Radotić, Ksenija, "ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING" in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research (2023):40-46, https://hdl.handle.net/21.15107/rcub_rimsi_2366 .