Mitochondrial nanomotion measured by optical microscopy
Autori
Parmar, PriyankaVillalba, Maria Ines
Seiji, Alexandre
Huber, Horii
Kalauzi, Aleksandar
Bartolić, Dragana
Radotić, Ksenija
Willaert, Ronnie
MacFabe, Derrick
Kasas, Sandor
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sen...sitivity, and is label- and attachment-free.
Ključne reči:
optical nanomotion / mitochondria / rotenone / metabolic substrates / short chain fatty acidsIzvor:
Frontiers in Microbiology, 2023, 14, 1133773Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200053 (Univerzitet u Beogradu, Institut za multidisciplinarna istraživanja) (RS-MESTD-inst-2020-200053)
- FWO, grant numbers I002620
- FWO-SNSF, grant number 310030L_197946
- SNSF grants CRSII5_173863
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Parmar, Priyanka AU - Villalba, Maria Ines AU - Seiji, Alexandre AU - Huber, Horii AU - Kalauzi, Aleksandar AU - Bartolić, Dragana AU - Radotić, Ksenija AU - Willaert, Ronnie AU - MacFabe, Derrick AU - Kasas, Sandor PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2183 AB - Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free. T2 - Frontiers in Microbiology T1 - Mitochondrial nanomotion measured by optical microscopy IS - 1133773 VL - 14 DO - 10.3389/fmicb.2023.1133773 ER -
@article{ author = "Parmar, Priyanka and Villalba, Maria Ines and Seiji, Alexandre and Huber, Horii and Kalauzi, Aleksandar and Bartolić, Dragana and Radotić, Ksenija and Willaert, Ronnie and MacFabe, Derrick and Kasas, Sandor", year = "2023", abstract = "Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free.", journal = "Frontiers in Microbiology", title = "Mitochondrial nanomotion measured by optical microscopy", number = "1133773", volume = "14", doi = "10.3389/fmicb.2023.1133773" }
Parmar, P., Villalba, M. I., Seiji, A., Huber, H., Kalauzi, A., Bartolić, D., Radotić, K., Willaert, R., MacFabe, D.,& Kasas, S.. (2023). Mitochondrial nanomotion measured by optical microscopy. in Frontiers in Microbiology, 14(1133773). https://doi.org/10.3389/fmicb.2023.1133773
Parmar P, Villalba MI, Seiji A, Huber H, Kalauzi A, Bartolić D, Radotić K, Willaert R, MacFabe D, Kasas S. Mitochondrial nanomotion measured by optical microscopy. in Frontiers in Microbiology. 2023;14(1133773). doi:10.3389/fmicb.2023.1133773 .
Parmar, Priyanka, Villalba, Maria Ines, Seiji, Alexandre, Huber, Horii, Kalauzi, Aleksandar, Bartolić, Dragana, Radotić, Ksenija, Willaert, Ronnie, MacFabe, Derrick, Kasas, Sandor, "Mitochondrial nanomotion measured by optical microscopy" in Frontiers in Microbiology, 14, no. 1133773 (2023), https://doi.org/10.3389/fmicb.2023.1133773 . .