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Is There Communication? Access to Information by Persons with Disabilities in Serbia

Masliković, Dejan; Tomić, Bojan

(Centre for Demographic Research of the National Institute of Social Sciences, 2024)

TY  - JOUR
AU  - Masliković, Dejan
AU  - Tomić, Bojan
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3217
AB  - Persons with disabilities often face barriers that hinder their ability to access information effectively. This study presents results of a survey which was conducted in the second half of 2022, on the topic of the accessibility of information for persons with disabilities in Serbia, as well as their means of communication. A convenience sample of 111 individuals with physical and/or sensory disabilities, with basic digital literacy, aged 18 to 59, was surveyed through the mediation of associations of persons with disabilities. The findings reveal the usage patterns, preferences, and challenges faced by individuals with disabilities in accessing information. It has been found that the internet (99.10%), social networks (91.89%), and specialized platforms (79.28%) are prominent sources of information. Although specialized applications for communication and telephone communication are widely used, personal contact communication (96.40% very often or often) is still the prevalent method of communication. Public institutions and state authorities are perceived as inadequately adapted to the needs of individuals with disabilities. The findings underscore the need for continued efforts to improve accessibility infrastructure and implement inclusive design principles to bridge the information gap for individuals with disabilities in Serbia.
AB  - Uspostavljanje komunikacije predstavlja tranziciju iz individualnog ka kolektivnom, što je važan korak u procesu formiranja uređenog društva. Postizanje zadovoljavajućeg nivoa komunikacije za sve članove društva je imperativ u 21. veku. Poznato je da osobe sa invaliditetom otežano ostvaruju komunikaciju sa okolinom, i da se često suočavaju sa preprekama koje ih sprečavaju da efikasno pristupe informacijama. U tom smislu obaveza svake države je da obezbedi zakonsku regulativu za omogućavanje pristupačnosti informacija. U Republici Srbiji postoji zakonski i tehnološki okvir kojim se reguliše pristupačnost. Ova studija predstavlja rezultate istraživanja o dostupnosti informacija osobama sa invaliditetom u Srbiji, i o obrascima njihove komunikacije. Na osnovu istraživanja koje je izvršeno posredstvom udruženja osoba sa invaliditetom na uzorku od 111 osoba sa fizičkim i/ili senzornim invaliditetom starosti od 18 do 59 godina dat je uvid u obrasce komunikacije i obrasce pristupa informacijama, prioritete koje ove osobe imaju u pogledu komunikacije i pristupa informacijama, i izazove sa kojima se suočavaju. Predstavljeni su rezultati istraživanja o dostupnosti sredstava informisanja, i o mogućnostima da osobe sa invaliditetom poboljšaju svoju situaciju u pogledu pristupa informacijama. Utvrđeno je da su internet (99,10%), društvene mreže (91,89%) i specijalizovane platforme (79,28%) prominentni izvori informacija. Iako su specijalizovane aplikacije za komunikaciju i telefonska komunikacija u širokoj upotrebi, lični kontakt (96,40% je odgovorilo sa vrlo često i često) je i dalje preovlađujući način komunikacije. Javne institucije i državni organi se percipiraju kao neadekvatno prilagođeni potrebama osoba sa invaliditetom. Ovi rezultati naglašavaju potrebu za sprovođenjem kontinuiranih napora sa ciljem poboljšanja infrastrukture u Srbiji u cilju obezbeđivanja pristupačnosti, i potrebu za primenom principa inkluzivnog dizajna kako bi se premostio jaz između osoba sa invaliditetom i osoba koje nemaju invaliditet, u pogledu pristupa informacijama.
PB  - Centre for Demographic Research of the National Institute of Social Sciences
T2  - Stanovništvo
T1  - Is There Communication? Access to Information by Persons with Disabilities in Serbia
T1  - Da li komunikacija postoji? Pristup informacijama u Srbiji za osobe sa invaliditetom
EP  - 166
IS  - 1
SP  - 153
VL  - 62
DO  - 10.59954/stnv.563
ER  - 
@article{
author = "Masliković, Dejan and Tomić, Bojan",
year = "2024",
abstract = "Persons with disabilities often face barriers that hinder their ability to access information effectively. This study presents results of a survey which was conducted in the second half of 2022, on the topic of the accessibility of information for persons with disabilities in Serbia, as well as their means of communication. A convenience sample of 111 individuals with physical and/or sensory disabilities, with basic digital literacy, aged 18 to 59, was surveyed through the mediation of associations of persons with disabilities. The findings reveal the usage patterns, preferences, and challenges faced by individuals with disabilities in accessing information. It has been found that the internet (99.10%), social networks (91.89%), and specialized platforms (79.28%) are prominent sources of information. Although specialized applications for communication and telephone communication are widely used, personal contact communication (96.40% very often or often) is still the prevalent method of communication. Public institutions and state authorities are perceived as inadequately adapted to the needs of individuals with disabilities. The findings underscore the need for continued efforts to improve accessibility infrastructure and implement inclusive design principles to bridge the information gap for individuals with disabilities in Serbia., Uspostavljanje komunikacije predstavlja tranziciju iz individualnog ka kolektivnom, što je važan korak u procesu formiranja uređenog društva. Postizanje zadovoljavajućeg nivoa komunikacije za sve članove društva je imperativ u 21. veku. Poznato je da osobe sa invaliditetom otežano ostvaruju komunikaciju sa okolinom, i da se često suočavaju sa preprekama koje ih sprečavaju da efikasno pristupe informacijama. U tom smislu obaveza svake države je da obezbedi zakonsku regulativu za omogućavanje pristupačnosti informacija. U Republici Srbiji postoji zakonski i tehnološki okvir kojim se reguliše pristupačnost. Ova studija predstavlja rezultate istraživanja o dostupnosti informacija osobama sa invaliditetom u Srbiji, i o obrascima njihove komunikacije. Na osnovu istraživanja koje je izvršeno posredstvom udruženja osoba sa invaliditetom na uzorku od 111 osoba sa fizičkim i/ili senzornim invaliditetom starosti od 18 do 59 godina dat je uvid u obrasce komunikacije i obrasce pristupa informacijama, prioritete koje ove osobe imaju u pogledu komunikacije i pristupa informacijama, i izazove sa kojima se suočavaju. Predstavljeni su rezultati istraživanja o dostupnosti sredstava informisanja, i o mogućnostima da osobe sa invaliditetom poboljšaju svoju situaciju u pogledu pristupa informacijama. Utvrđeno je da su internet (99,10%), društvene mreže (91,89%) i specijalizovane platforme (79,28%) prominentni izvori informacija. Iako su specijalizovane aplikacije za komunikaciju i telefonska komunikacija u širokoj upotrebi, lični kontakt (96,40% je odgovorilo sa vrlo često i često) je i dalje preovlađujući način komunikacije. Javne institucije i državni organi se percipiraju kao neadekvatno prilagođeni potrebama osoba sa invaliditetom. Ovi rezultati naglašavaju potrebu za sprovođenjem kontinuiranih napora sa ciljem poboljšanja infrastrukture u Srbiji u cilju obezbeđivanja pristupačnosti, i potrebu za primenom principa inkluzivnog dizajna kako bi se premostio jaz između osoba sa invaliditetom i osoba koje nemaju invaliditet, u pogledu pristupa informacijama.",
publisher = "Centre for Demographic Research of the National Institute of Social Sciences",
journal = "Stanovništvo",
title = "Is There Communication? Access to Information by Persons with Disabilities in Serbia, Da li komunikacija postoji? Pristup informacijama u Srbiji za osobe sa invaliditetom",
pages = "166-153",
number = "1",
volume = "62",
doi = "10.59954/stnv.563"
}
Masliković, D.,& Tomić, B.. (2024). Is There Communication? Access to Information by Persons with Disabilities in Serbia. in Stanovništvo
Centre for Demographic Research of the National Institute of Social Sciences., 62(1), 153-166.
https://doi.org/10.59954/stnv.563
Masliković D, Tomić B. Is There Communication? Access to Information by Persons with Disabilities in Serbia. in Stanovništvo. 2024;62(1):153-166.
doi:10.59954/stnv.563 .
Masliković, Dejan, Tomić, Bojan, "Is There Communication? Access to Information by Persons with Disabilities in Serbia" in Stanovništvo, 62, no. 1 (2024):153-166,
https://doi.org/10.59954/stnv.563 . .

Sakralna geografija i kulturna baština – slučaj planina

Tomić, Bojan; Tomić, Milica M.

(Centar za crkvene studije / The Centre for Church Studies, 2024)

TY  - JOUR
AU  - Tomić, Bojan
AU  - Tomić, Milica M.
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3218
AB  - U radu se proučavaju planine, brda i gore u kontekstu sakralnog geokulturnog nasleđa. Neki od ključnih događaja u istoriji judeohrišćanstva odigrali su se na planinama, zbog čega se sakralna geografija proučava objedinjeno sa kulturnom istorijom. Istorija jevrejskog naroda opisana u Starom zavetu neodvojiva je od planina, posebno od visoke Sinajske gore, dok reljefna uzvišenja iz Novog zaveta (Maslinska gora, Tavor, Golgota) spadaju u niske planine. Visoke planine i teško pristupačni predeli brdsko-planinskog reljefa lokacije su mnogobrojnih pravoslavnih svetinja koje su na listi Uneskove kulturne baštine, poput manastira Svete Katarine, Svete gore Atonske, Meteora, srednjovekovnog grada Lalibela u Etiopiji i Rilskog manastira.
AB  - In this paper, mountains and hills are being studied in the context of sacred geocultural heritage. Some of the key events in the history of Judeo-Christianity took place in mountains, which is why sacred geography is studied jointly with cultural history. History of the Jewish people described in the Old Testament is inseparable from the mountains, especially from the high Mount Sinai, while the relief elevations from the New Testament (the Mount of Olives, Tabor, Golgotha) belong to low mountains. In high mountains and hard-to-reach mountainous relief areas there are numerous Orthodox holy sites listed as the UNESCO cultural heritage, such as St. Catherine's Monastery, Mount Athos, Meteora, the medieval city of Lalibela in Ethiopia, and the Rila Monastery.
PB  - Centar za crkvene studije / The Centre for Church Studies
PB  - Međunarodni centar za pravoslavne studije / International Center for Othodox Studies
T2  - Crkvene studije
T1  - Sakralna geografija i kulturna baština – slučaj planina
T1  - Sacred geography and cultural heritage – the case of mountains
EP  - 517
SP  - 507
VL  - 21
DO  - 10.18485/ccs_cs.2024.21.21.33
ER  - 
@article{
author = "Tomić, Bojan and Tomić, Milica M.",
year = "2024",
abstract = "U radu se proučavaju planine, brda i gore u kontekstu sakralnog geokulturnog nasleđa. Neki od ključnih događaja u istoriji judeohrišćanstva odigrali su se na planinama, zbog čega se sakralna geografija proučava objedinjeno sa kulturnom istorijom. Istorija jevrejskog naroda opisana u Starom zavetu neodvojiva je od planina, posebno od visoke Sinajske gore, dok reljefna uzvišenja iz Novog zaveta (Maslinska gora, Tavor, Golgota) spadaju u niske planine. Visoke planine i teško pristupačni predeli brdsko-planinskog reljefa lokacije su mnogobrojnih pravoslavnih svetinja koje su na listi Uneskove kulturne baštine, poput manastira Svete Katarine, Svete gore Atonske, Meteora, srednjovekovnog grada Lalibela u Etiopiji i Rilskog manastira., In this paper, mountains and hills are being studied in the context of sacred geocultural heritage. Some of the key events in the history of Judeo-Christianity took place in mountains, which is why sacred geography is studied jointly with cultural history. History of the Jewish people described in the Old Testament is inseparable from the mountains, especially from the high Mount Sinai, while the relief elevations from the New Testament (the Mount of Olives, Tabor, Golgotha) belong to low mountains. In high mountains and hard-to-reach mountainous relief areas there are numerous Orthodox holy sites listed as the UNESCO cultural heritage, such as St. Catherine's Monastery, Mount Athos, Meteora, the medieval city of Lalibela in Ethiopia, and the Rila Monastery.",
publisher = "Centar za crkvene studije / The Centre for Church Studies, Međunarodni centar za pravoslavne studije / International Center for Othodox Studies",
journal = "Crkvene studije",
title = "Sakralna geografija i kulturna baština – slučaj planina, Sacred geography and cultural heritage – the case of mountains",
pages = "517-507",
volume = "21",
doi = "10.18485/ccs_cs.2024.21.21.33"
}
Tomić, B.,& Tomić, M. M.. (2024). Sakralna geografija i kulturna baština – slučaj planina. in Crkvene studije
Centar za crkvene studije / The Centre for Church Studies., 21, 507-517.
https://doi.org/10.18485/ccs_cs.2024.21.21.33
Tomić B, Tomić MM. Sakralna geografija i kulturna baština – slučaj planina. in Crkvene studije. 2024;21:507-517.
doi:10.18485/ccs_cs.2024.21.21.33 .
Tomić, Bojan, Tomić, Milica M., "Sakralna geografija i kulturna baština – slučaj planina" in Crkvene studije, 21 (2024):507-517,
https://doi.org/10.18485/ccs_cs.2024.21.21.33 . .

KONCENTRACIJE PESTICIDA I PCB-A U TRI CIPRINIDNE VRSTE RIBA SA AKUMULACIJE MEĐUVRŠJE

Skorić, Stefan; Nikolić, Dušan

(Srpsko društvo za zaštitu voda, 2024)

TY  - CONF
AU  - Skorić, Stefan
AU  - Nikolić, Dušan
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3215
AB  - Tokom ihtioloških istraživanja na akumulaciji Međuvršje 2016. godine analiziran je sadržaj
4 vrste pesticida (aldrin, 4,4'-DDT, 4,4'-DDD, 4,4'-DDE) i 6 vrsta polihlorovanih
bifenila (PCB - 28, 52, 101, 138, 153, 180, kao i ukupni PCB) u mišićnom tkivu tri
ciprinidne vrste riba (klen, skobalj i bodorka) koje imaju ribolovni značaj i koriste se u
ljudskoj ishrani. Vrednosti svih analiziranih jedinjenja su bile ispod vresdnosti dozvoljenih
nacionalnom legislativom te se stoga može smatrati bezbedna konzumacija ovih vrsta riba.
AB  - In 2016, during ichthyological research on the Medjuvršje reservoir, the content of 4 types
of pesticides (aldrin, 4,4'-DDT, 4,4'-DDD, 4,4'-DDE) and 6 types of polychlorinated
biphenyls (PCB - 28, 52) were analyzed. , 101, 138, 153, 180, as well as total PCBs) in the
muscle tissue of three cyprinid fish species (chub, nase and roach) that have fishing
importance and are used in human nutrition. The values of all analyzed compounds were
below the values allowed by the national legislation, and therefore it can be considered safe
consumption of thesefish species.
PB  - Srpsko društvo za zaštitu voda
C3  - 53RD ANNUAL CONFERENCE OF THE SERBIAN WATER POLLUTION CONTROL SOCIETY "WATER 2024" CONFERENCE PROCEEDINGS
T1  - KONCENTRACIJE PESTICIDA I PCB-A U TRI CIPRINIDNE VRSTE RIBA SA AKUMULACIJE MEĐUVRŠJE
T1  - CONCENTRATIONS OF PESTICIDES AND PCBs IN THREE CYPRINID FISH SPECIES FROM THE MEDJUVRSJE RESERVOIR
EP  - 160
SP  - 155
DO  - 10.46793/VODA24.155S
ER  - 
@conference{
author = "Skorić, Stefan and Nikolić, Dušan",
year = "2024",
abstract = "Tokom ihtioloških istraživanja na akumulaciji Međuvršje 2016. godine analiziran je sadržaj
4 vrste pesticida (aldrin, 4,4'-DDT, 4,4'-DDD, 4,4'-DDE) i 6 vrsta polihlorovanih
bifenila (PCB - 28, 52, 101, 138, 153, 180, kao i ukupni PCB) u mišićnom tkivu tri
ciprinidne vrste riba (klen, skobalj i bodorka) koje imaju ribolovni značaj i koriste se u
ljudskoj ishrani. Vrednosti svih analiziranih jedinjenja su bile ispod vresdnosti dozvoljenih
nacionalnom legislativom te se stoga može smatrati bezbedna konzumacija ovih vrsta riba., In 2016, during ichthyological research on the Medjuvršje reservoir, the content of 4 types
of pesticides (aldrin, 4,4'-DDT, 4,4'-DDD, 4,4'-DDE) and 6 types of polychlorinated
biphenyls (PCB - 28, 52) were analyzed. , 101, 138, 153, 180, as well as total PCBs) in the
muscle tissue of three cyprinid fish species (chub, nase and roach) that have fishing
importance and are used in human nutrition. The values of all analyzed compounds were
below the values allowed by the national legislation, and therefore it can be considered safe
consumption of thesefish species.",
publisher = "Srpsko društvo za zaštitu voda",
journal = "53RD ANNUAL CONFERENCE OF THE SERBIAN WATER POLLUTION CONTROL SOCIETY "WATER 2024" CONFERENCE PROCEEDINGS",
title = "KONCENTRACIJE PESTICIDA I PCB-A U TRI CIPRINIDNE VRSTE RIBA SA AKUMULACIJE MEĐUVRŠJE, CONCENTRATIONS OF PESTICIDES AND PCBs IN THREE CYPRINID FISH SPECIES FROM THE MEDJUVRSJE RESERVOIR",
pages = "160-155",
doi = "10.46793/VODA24.155S"
}
Skorić, S.,& Nikolić, D.. (2024). KONCENTRACIJE PESTICIDA I PCB-A U TRI CIPRINIDNE VRSTE RIBA SA AKUMULACIJE MEĐUVRŠJE. in 53RD ANNUAL CONFERENCE OF THE SERBIAN WATER POLLUTION CONTROL SOCIETY "WATER 2024" CONFERENCE PROCEEDINGS
Srpsko društvo za zaštitu voda., 155-160.
https://doi.org/10.46793/VODA24.155S
Skorić S, Nikolić D. KONCENTRACIJE PESTICIDA I PCB-A U TRI CIPRINIDNE VRSTE RIBA SA AKUMULACIJE MEĐUVRŠJE. in 53RD ANNUAL CONFERENCE OF THE SERBIAN WATER POLLUTION CONTROL SOCIETY "WATER 2024" CONFERENCE PROCEEDINGS. 2024;:155-160.
doi:10.46793/VODA24.155S .
Skorić, Stefan, Nikolić, Dušan, "KONCENTRACIJE PESTICIDA I PCB-A U TRI CIPRINIDNE VRSTE RIBA SA AKUMULACIJE MEĐUVRŠJE" in 53RD ANNUAL CONFERENCE OF THE SERBIAN WATER POLLUTION CONTROL SOCIETY "WATER 2024" CONFERENCE PROCEEDINGS (2024):155-160,
https://doi.org/10.46793/VODA24.155S . .

Laboratory Experimental Setup for High Voltage Corona Polarization of Thin PVDF Films

Despotovic, Zeljko V.; Vijatović Petrović, Mirjana; Bobić, Jelena

(IEEE, 2024)

TY  - CONF
AU  - Despotovic, Zeljko V.
AU  - Vijatović Petrović, Mirjana
AU  - Bobić, Jelena
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3216
AB  - Ferroelectric thin films based on the polymer material
polyvinylidene fluoride (PVDF) can potentially have a wide range
of applications, due to the strong connection between their
electrical, mechanical and thermal properties. PVDF thin films
can be applied in memory devices. The most widespread
application of these elements is in piezoelectric sensors, actuators
and energy harvesters from mechanical vibrations and
mechanical impacts (the so-called "energy harvesting"). A very
important operation in the production and testing of thin PVDF
films is the polarization of the ferroelectric domains in the
material. Similar to the case of ferromagnetic materials, after the
production of the polymer material, the polarization domains in
it are oriented stochastically, so it is necessary to perform their
orientation by direction and course. For this reason, the
ferroelectric sample is exposed to relatively high electric field
strengths of (30÷100) kV/cm in order to orient and direct the
domains. The paper presents the practical realization of the
laboratory experimental setup of the system for high-voltage
polarization of ultra-thin PVDF films by means of a high-voltage
corona. Controlled corona is achieved in a hermetically sealed,
temperature controlled and electrically isolated chamber. Finally,
some key experimental results concerning high-voltage
polarization are given.
PB  - IEEE
C3  - 23rd International Symposium INFOTEH-JAHORINA, 20-22 March 2024
T1  - Laboratory Experimental Setup for High Voltage Corona Polarization of Thin PVDF Films
DO  - 10.1109/INFOTEH60418.2024.10496040
ER  - 
@conference{
author = "Despotovic, Zeljko V. and Vijatović Petrović, Mirjana and Bobić, Jelena",
year = "2024",
abstract = "Ferroelectric thin films based on the polymer material
polyvinylidene fluoride (PVDF) can potentially have a wide range
of applications, due to the strong connection between their
electrical, mechanical and thermal properties. PVDF thin films
can be applied in memory devices. The most widespread
application of these elements is in piezoelectric sensors, actuators
and energy harvesters from mechanical vibrations and
mechanical impacts (the so-called "energy harvesting"). A very
important operation in the production and testing of thin PVDF
films is the polarization of the ferroelectric domains in the
material. Similar to the case of ferromagnetic materials, after the
production of the polymer material, the polarization domains in
it are oriented stochastically, so it is necessary to perform their
orientation by direction and course. For this reason, the
ferroelectric sample is exposed to relatively high electric field
strengths of (30÷100) kV/cm in order to orient and direct the
domains. The paper presents the practical realization of the
laboratory experimental setup of the system for high-voltage
polarization of ultra-thin PVDF films by means of a high-voltage
corona. Controlled corona is achieved in a hermetically sealed,
temperature controlled and electrically isolated chamber. Finally,
some key experimental results concerning high-voltage
polarization are given.",
publisher = "IEEE",
journal = "23rd International Symposium INFOTEH-JAHORINA, 20-22 March 2024",
title = "Laboratory Experimental Setup for High Voltage Corona Polarization of Thin PVDF Films",
doi = "10.1109/INFOTEH60418.2024.10496040"
}
Despotovic, Z. V., Vijatović Petrović, M.,& Bobić, J.. (2024). Laboratory Experimental Setup for High Voltage Corona Polarization of Thin PVDF Films. in 23rd International Symposium INFOTEH-JAHORINA, 20-22 March 2024
IEEE..
https://doi.org/10.1109/INFOTEH60418.2024.10496040
Despotovic ZV, Vijatović Petrović M, Bobić J. Laboratory Experimental Setup for High Voltage Corona Polarization of Thin PVDF Films. in 23rd International Symposium INFOTEH-JAHORINA, 20-22 March 2024. 2024;.
doi:10.1109/INFOTEH60418.2024.10496040 .
Despotovic, Zeljko V., Vijatović Petrović, Mirjana, Bobić, Jelena, "Laboratory Experimental Setup for High Voltage Corona Polarization of Thin PVDF Films" in 23rd International Symposium INFOTEH-JAHORINA, 20-22 March 2024 (2024),
https://doi.org/10.1109/INFOTEH60418.2024.10496040 . .

Uklanjanje fenola i tekstilnih boja iz otpadnih voda imobilizovanim peroksidazama iz rena (Armoracia rusticana) i gljive bele truleži (Phanerochaete chrysosporium)

Surudžić, Nevena

(2024)

TY  - THES
AU  - Surudžić, Nevena
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3214
AB  - Sve veći globalni problem predstavlja zagađenje vodenih sistema razičitim otpadnim
materijama, pre svega fenolnim jedinjenjima i organskim bojama ispuštenim iz mnogobrojnih
industrijskih postrojenja. S obzirom da su fenoli označeni kao najčešći zagađivači voda širom
sveta, pronalazak metoda za njihovo uklanjanje predstavlja jedan od najvećih izazova
savremene naučne zajednice. Različiti biokatalizatori su pronašli primenu u ove svrhe, a među
njima je posebno mesto zauzela peroksidaza iz rena (HRP). Kao najveće ograničenje ove metode
navodi se inaktivacija enzima, koja se može prevazići njegovom imobilizacijom na različitim
nosačima.
U ovom radu su razvijene metode za uklanjanje fenola i degradaciju tekstilnih boja
imobilizovanom peroksidazom iz rena i ćelijskim zidovima sa lignin-peroksidazom (LiP). Kao
nosači za imobilizaciju su korišćeni oksidovani alginati modifikovani tiraminom, makroporozni
kopolimeri različite veličine pora, kao i neorganski glineni nosač – sepiolit.
Tiramin-alginatne mikro-kuglice su iskorišćene za enkapsulaciju peroksidaze iz rena
primenom kuplovane emulziono-polimerizacione reakcije. Ispitan je uticaj stepena
modifikacije alginata tiraminom na specifičnu aktivnost enzima. Ustanovljeno je da sa
povećanjem koncentracije perjodata korišćenog za oksidaciju alginata od 2,5 do 20 mol% pre
modifikacije tiraminom dolazi do postepenog povećanja specifične aktivnosti.
Imobilizovana HRP je potom upotrebljena za uklanjanje fenola u šaržnom reaktoru.
Optimizovani su reakcioni uslovi sa ciljem postizanja visokog stepena efikasnosti i poboljšane
kontinualne primene. Za interno generisanje vodonik-peroksida (H2O2) tokom oksidacije
fenola primenjen je sistem sastavljen od glukoze i glukoza-oksidaze. Znatno veći stepen
uklanjanja fenola (96%) iz reakcionog sistema za 6 h je postignut primenom interne dostave
H2O2, dok se njegovim direktnim dodavanjem značajno smanjuje efikasnost uklanjanja fenola
na 42%. Primenom glukoza-oksidaze u koncentraciji od 0,187 U/mL i 4 mmol/L glukoze
postiže se najveća efikasnost, kao i korišćenjem 20 mol% oksidovanog i modifikovanog tiraminalginata
za enkapsulaciju HRP.
Degradacija tekstilnih boja ostvarena je i primenom ćelijskih zidova sa ligninperoksidazom
(LiP) zarobljenih u kalcijum-alginatnom hidrogelu. LiP je imobilizovana na
tiramin-alginatne mikro-kuglice. Inkubacijom enkapsuliranih ćelijskih zidova u rastvorima
boja Evans Blue i Amido Black 10B tokom 48 h i merenjem njihove aktivnosti u određenim
vremenskim intervalima uočeno je postepeno povećanje procenta degradacije sa periodom
inkubacije. Nakon maksimalne inkubacije 86% boje Evans Blue se dekolorizuje, dok taj
procenat za Amido Black 10B iznosi 84%. Ponovnom upotrebom ćelijskih zidova sa LiP
ostvarena je efikasna degradacija navedenih boja tokom 12 uzastopnih ciklusa.
Makroporozni kopolimeri sastavljeni od glicidil metakrilata i etilen glikol dimetakrilata
veličine čestica od približno 2 μm i različite veličine pora (150–310 nm) su sintetisani
disperzionom polimerizacijom i modifikovani etilendiaminom. Dve različite metode,
perjodatna i glutaraldehidna, su korišćene za imobilizaciju peroksidaze iz rena na ovim
polimerima.
Aktivnost imobilizovanog enzima je u direktnoj vezi sa veličinom pora na nosaču
korišćenom za imobilizaciju. Imobilizacijom HRP perjodatnom metodom na poli(GMA-co-
EGDMA) veličine pora od 234 nm dobijena je specifična aktivnost od 9,65 U/g nosača, dok je
HRP na kopolimeru sa porama od 297 nm imala specifičnu aktivnost 8,94 U/g nosača. Takođe
je došlo i do povećanja stabilnosti imobilizovane peroksidaze na 65 °C u vodenom rastvoru kao
i u organskom rastvaraču.
Dobru stabilnost na različitim pH vrednostima, kao i povećanu Km vrednost tokom
oksidacije pirogalola, pokazala je peroksidaza imobilizovana na makroporoznom kopolimeru
sa veličinom pora od 234 nm, koja je imala i najveću specifičnu aktivnost. Nakon 5 ciklusa
ponovne upotrebe za oksidaciju pirogalola zadržano je 80% inicijalne aktivnosti. Imobilizovani
enzim skladišten 14 dana u odgovarajućem puferu zadržao je 98% aktivnosti. Značajna
efikasnost uklanjanja fenola pri optimizovanim uslovima za dostavu vodonik-peroksida u
reakcioni sistem je postignuta primenom HRP imobilizovane na kopolimeru sa porama veličine
234 nm.
Sepiolit je primenjen za adsorpciju nativne HRP i HRP oksidovane natrijum-perjodatom.
Dodate su različite količine oba enzima po gramu sepiolitnog nosača. Sa povećanjem količine
peroksidaze povećava se specifična aktivnost imobilizovanog enzima. Najveća aktivnost je
postignuta pri imobilizaciji 15 mg perjodatno-oksidovane peroksidaze po gramu sepiolita.
Testirane su temperaturna i operativna stabilnost imobilizovanih peroksidaza, kao i stabilnosti
na različitim pH vrednostima. Oksidovana peroksidaza je zadržala 42% inicijalne aktivnosti
nakon 4 uzastopna ciklusa oksidacije pirogalola.
AB  - A growing global concern is the pollution of water courses with various waste materials,
primarly phenolic compounds and organic dyes released from numerous industrial plants.
Since phenols are designated as the most common water pollutants worldwide, finding
methods for their removal is nowadays one of the biggest challenges for the broad scientific
community. To this end, various biocatalysts have been used, among them Horseradish
peroxidase (HRP) has been in prime position. The main limitation of this method is an enzyme
inactivation, which can be overcome by its immobilization on different carriers.
In this study, various methods for the removal of phenol and degradation of textile dyes by
immobilized Horseradish peroxidase and cell walls with lignin peroxidase (LiP) were
developed. Oxidized alginates modified with tyramine, macroporous copolymers with different
pore characteristics, as well as inorganic clay mineral – sepiolite, were used as carriers in
immobilization.
Tyramine-alginate micro-beads were used for the encapsulation of peroxidase by coupled
emulsion polymerization reaction. An effect of a degree of the alginate modification by tyramine
on the specific activity of enzyme was examined. A gradual increase in the specific activity, with
an increase in the concentration of periodate used for oxidation of alginate from 2.5 to 20 mol%
prior to modification by tyramine, was observed.
Immobilized HRP was then used for the removal of phenol in a batch reactor. Reaction
conditions were optimized with an aim of achieving a high degree of efficiency and improved
reusability. A system composed of glucose and glucose oxidase was used for internal generation
of hydrogen peroxide (H2O2). When internal delivery of H2O2 was employed, 96% of phenol was
removed from the reaction system in 6 h, whereas its straightforward addition significantly
reduced the efficiency of phenol removal (42%). The highest efficiency was achieved by using
glucose oxidase at the concentration of 0.187 U/mL and 4 mmol/L of glucose, as well as by using
20 mol% periodate-oxidized and modified tyramine-alginate for HRP encapsulation.
Degradation of textile dyes was performed by employing cell walls with lignin peroxidase
(LiP) entrapped in a calcium-alginate hydrogel. LiP was also imobillized on tryamine-alginate
micro-beads. By incubating encapsulated cell walls in Evans Blue and Amido Black 10B dye
solutions for 48 h and measuring their activity in certain time intervals, a gradual increase in
the degree of degradation with incubation period was observed. Maximal incubation in Evans
Blue dye leads to 86% decolorization, whereas that percent for Amido Black 10B is 84%
Efficient degradation of previously mentioned dyes was achieved within 12 consecutive cycles
by reusing cell walls with LiP.
Macroporous copolymers composed of glycidyl methacrylate and ethylene glycol
dimethacrylate with a particle size of approximately 2 μm and different pore sizes (150–
310 nm) were synthesized by dispersion polymerization and modified with ethylenediamine.
Two methods, periodate and glutaraldehyde method, were used for the immobilization of horseradish peroxidase onto these polymers.
The activity of immobilized enzyme is affected by the pore size of the carrier used for
immobilization. By immobilizing HRP using the periodate method onto poly (GMA-co-EGDMA)
copolymers with the pore size diameter of 234 nm specific activity of 9.65 U/g of carrier was
obtained, whereas HRP immobilized onto carrier with the pore size diameter of 297 nm showed
specific activity of 8.94 U/g of carrier. Also, the stability of immobilized peroxidase at 65 °C, as
well as in an organic solvent, was increased.
Peroxidase immobilized onto macroporous copolymer with a pore size diameter of 234 nm
and the highest specific activity also showed a good stability at different pH values, as well as
the increased Km value during the oxidation of pyrogallol. Eighty percent of the initial activity
was retained after 5 consecutive cycles of pyrogallol oxidation. After storing the immobilized
enzyme for 14 days in appropriate buffer, 98% of its activity was preserved. A significant
efficiency of phenol removal under optimized conditions for the delivery of hydrogen peroxide
into the reaction mixture was achieved by applying HRP immobilized onto copolymer with the
pore diameter of 234 nm.
Sepiolite was used for the adsorption of native HRP and HRP oxidized with sodium
periodate. Different amounts of both enzymes were added per gram of sepiolite carrier.
Increase in the amount of peroxidase leads to the increase in the specific activity of immobilized
enzyme. The highest activity was achieved when 15 mg of periodate-oxidized peroxidase was
immobilized per gram of sepiolite. Temperature and operational stabilities of immobilized
peroxidases were tested. Oxidized peroxidase retained 42% of initial activity after
4 consecutive cycles of pyrogallol oxidation.
T2  - UNIVERZITET U BEOGRADU HEMIJSKI FAKULTET
T1  - Uklanjanje fenola i tekstilnih boja iz otpadnih voda imobilizovanim peroksidazama iz rena (Armoracia rusticana) i gljive bele truleži (Phanerochaete chrysosporium)
T1  - Phenol and textile dye removal from wastewater with immobilized peroxidases from Horseradish (Armoracia rusticana) and white rot fungi (Phanerochaete chrysosporium)
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3214
ER  - 
@phdthesis{
author = "Surudžić, Nevena",
year = "2024",
abstract = "Sve veći globalni problem predstavlja zagađenje vodenih sistema razičitim otpadnim
materijama, pre svega fenolnim jedinjenjima i organskim bojama ispuštenim iz mnogobrojnih
industrijskih postrojenja. S obzirom da su fenoli označeni kao najčešći zagađivači voda širom
sveta, pronalazak metoda za njihovo uklanjanje predstavlja jedan od najvećih izazova
savremene naučne zajednice. Različiti biokatalizatori su pronašli primenu u ove svrhe, a među
njima je posebno mesto zauzela peroksidaza iz rena (HRP). Kao najveće ograničenje ove metode
navodi se inaktivacija enzima, koja se može prevazići njegovom imobilizacijom na različitim
nosačima.
U ovom radu su razvijene metode za uklanjanje fenola i degradaciju tekstilnih boja
imobilizovanom peroksidazom iz rena i ćelijskim zidovima sa lignin-peroksidazom (LiP). Kao
nosači za imobilizaciju su korišćeni oksidovani alginati modifikovani tiraminom, makroporozni
kopolimeri različite veličine pora, kao i neorganski glineni nosač – sepiolit.
Tiramin-alginatne mikro-kuglice su iskorišćene za enkapsulaciju peroksidaze iz rena
primenom kuplovane emulziono-polimerizacione reakcije. Ispitan je uticaj stepena
modifikacije alginata tiraminom na specifičnu aktivnost enzima. Ustanovljeno je da sa
povećanjem koncentracije perjodata korišćenog za oksidaciju alginata od 2,5 do 20 mol% pre
modifikacije tiraminom dolazi do postepenog povećanja specifične aktivnosti.
Imobilizovana HRP je potom upotrebljena za uklanjanje fenola u šaržnom reaktoru.
Optimizovani su reakcioni uslovi sa ciljem postizanja visokog stepena efikasnosti i poboljšane
kontinualne primene. Za interno generisanje vodonik-peroksida (H2O2) tokom oksidacije
fenola primenjen je sistem sastavljen od glukoze i glukoza-oksidaze. Znatno veći stepen
uklanjanja fenola (96%) iz reakcionog sistema za 6 h je postignut primenom interne dostave
H2O2, dok se njegovim direktnim dodavanjem značajno smanjuje efikasnost uklanjanja fenola
na 42%. Primenom glukoza-oksidaze u koncentraciji od 0,187 U/mL i 4 mmol/L glukoze
postiže se najveća efikasnost, kao i korišćenjem 20 mol% oksidovanog i modifikovanog tiraminalginata
za enkapsulaciju HRP.
Degradacija tekstilnih boja ostvarena je i primenom ćelijskih zidova sa ligninperoksidazom
(LiP) zarobljenih u kalcijum-alginatnom hidrogelu. LiP je imobilizovana na
tiramin-alginatne mikro-kuglice. Inkubacijom enkapsuliranih ćelijskih zidova u rastvorima
boja Evans Blue i Amido Black 10B tokom 48 h i merenjem njihove aktivnosti u određenim
vremenskim intervalima uočeno je postepeno povećanje procenta degradacije sa periodom
inkubacije. Nakon maksimalne inkubacije 86% boje Evans Blue se dekolorizuje, dok taj
procenat za Amido Black 10B iznosi 84%. Ponovnom upotrebom ćelijskih zidova sa LiP
ostvarena je efikasna degradacija navedenih boja tokom 12 uzastopnih ciklusa.
Makroporozni kopolimeri sastavljeni od glicidil metakrilata i etilen glikol dimetakrilata
veličine čestica od približno 2 μm i različite veličine pora (150–310 nm) su sintetisani
disperzionom polimerizacijom i modifikovani etilendiaminom. Dve različite metode,
perjodatna i glutaraldehidna, su korišćene za imobilizaciju peroksidaze iz rena na ovim
polimerima.
Aktivnost imobilizovanog enzima je u direktnoj vezi sa veličinom pora na nosaču
korišćenom za imobilizaciju. Imobilizacijom HRP perjodatnom metodom na poli(GMA-co-
EGDMA) veličine pora od 234 nm dobijena je specifična aktivnost od 9,65 U/g nosača, dok je
HRP na kopolimeru sa porama od 297 nm imala specifičnu aktivnost 8,94 U/g nosača. Takođe
je došlo i do povećanja stabilnosti imobilizovane peroksidaze na 65 °C u vodenom rastvoru kao
i u organskom rastvaraču.
Dobru stabilnost na različitim pH vrednostima, kao i povećanu Km vrednost tokom
oksidacije pirogalola, pokazala je peroksidaza imobilizovana na makroporoznom kopolimeru
sa veličinom pora od 234 nm, koja je imala i najveću specifičnu aktivnost. Nakon 5 ciklusa
ponovne upotrebe za oksidaciju pirogalola zadržano je 80% inicijalne aktivnosti. Imobilizovani
enzim skladišten 14 dana u odgovarajućem puferu zadržao je 98% aktivnosti. Značajna
efikasnost uklanjanja fenola pri optimizovanim uslovima za dostavu vodonik-peroksida u
reakcioni sistem je postignuta primenom HRP imobilizovane na kopolimeru sa porama veličine
234 nm.
Sepiolit je primenjen za adsorpciju nativne HRP i HRP oksidovane natrijum-perjodatom.
Dodate su različite količine oba enzima po gramu sepiolitnog nosača. Sa povećanjem količine
peroksidaze povećava se specifična aktivnost imobilizovanog enzima. Najveća aktivnost je
postignuta pri imobilizaciji 15 mg perjodatno-oksidovane peroksidaze po gramu sepiolita.
Testirane su temperaturna i operativna stabilnost imobilizovanih peroksidaza, kao i stabilnosti
na različitim pH vrednostima. Oksidovana peroksidaza je zadržala 42% inicijalne aktivnosti
nakon 4 uzastopna ciklusa oksidacije pirogalola., A growing global concern is the pollution of water courses with various waste materials,
primarly phenolic compounds and organic dyes released from numerous industrial plants.
Since phenols are designated as the most common water pollutants worldwide, finding
methods for their removal is nowadays one of the biggest challenges for the broad scientific
community. To this end, various biocatalysts have been used, among them Horseradish
peroxidase (HRP) has been in prime position. The main limitation of this method is an enzyme
inactivation, which can be overcome by its immobilization on different carriers.
In this study, various methods for the removal of phenol and degradation of textile dyes by
immobilized Horseradish peroxidase and cell walls with lignin peroxidase (LiP) were
developed. Oxidized alginates modified with tyramine, macroporous copolymers with different
pore characteristics, as well as inorganic clay mineral – sepiolite, were used as carriers in
immobilization.
Tyramine-alginate micro-beads were used for the encapsulation of peroxidase by coupled
emulsion polymerization reaction. An effect of a degree of the alginate modification by tyramine
on the specific activity of enzyme was examined. A gradual increase in the specific activity, with
an increase in the concentration of periodate used for oxidation of alginate from 2.5 to 20 mol%
prior to modification by tyramine, was observed.
Immobilized HRP was then used for the removal of phenol in a batch reactor. Reaction
conditions were optimized with an aim of achieving a high degree of efficiency and improved
reusability. A system composed of glucose and glucose oxidase was used for internal generation
of hydrogen peroxide (H2O2). When internal delivery of H2O2 was employed, 96% of phenol was
removed from the reaction system in 6 h, whereas its straightforward addition significantly
reduced the efficiency of phenol removal (42%). The highest efficiency was achieved by using
glucose oxidase at the concentration of 0.187 U/mL and 4 mmol/L of glucose, as well as by using
20 mol% periodate-oxidized and modified tyramine-alginate for HRP encapsulation.
Degradation of textile dyes was performed by employing cell walls with lignin peroxidase
(LiP) entrapped in a calcium-alginate hydrogel. LiP was also imobillized on tryamine-alginate
micro-beads. By incubating encapsulated cell walls in Evans Blue and Amido Black 10B dye
solutions for 48 h and measuring their activity in certain time intervals, a gradual increase in
the degree of degradation with incubation period was observed. Maximal incubation in Evans
Blue dye leads to 86% decolorization, whereas that percent for Amido Black 10B is 84%
Efficient degradation of previously mentioned dyes was achieved within 12 consecutive cycles
by reusing cell walls with LiP.
Macroporous copolymers composed of glycidyl methacrylate and ethylene glycol
dimethacrylate with a particle size of approximately 2 μm and different pore sizes (150–
310 nm) were synthesized by dispersion polymerization and modified with ethylenediamine.
Two methods, periodate and glutaraldehyde method, were used for the immobilization of horseradish peroxidase onto these polymers.
The activity of immobilized enzyme is affected by the pore size of the carrier used for
immobilization. By immobilizing HRP using the periodate method onto poly (GMA-co-EGDMA)
copolymers with the pore size diameter of 234 nm specific activity of 9.65 U/g of carrier was
obtained, whereas HRP immobilized onto carrier with the pore size diameter of 297 nm showed
specific activity of 8.94 U/g of carrier. Also, the stability of immobilized peroxidase at 65 °C, as
well as in an organic solvent, was increased.
Peroxidase immobilized onto macroporous copolymer with a pore size diameter of 234 nm
and the highest specific activity also showed a good stability at different pH values, as well as
the increased Km value during the oxidation of pyrogallol. Eighty percent of the initial activity
was retained after 5 consecutive cycles of pyrogallol oxidation. After storing the immobilized
enzyme for 14 days in appropriate buffer, 98% of its activity was preserved. A significant
efficiency of phenol removal under optimized conditions for the delivery of hydrogen peroxide
into the reaction mixture was achieved by applying HRP immobilized onto copolymer with the
pore diameter of 234 nm.
Sepiolite was used for the adsorption of native HRP and HRP oxidized with sodium
periodate. Different amounts of both enzymes were added per gram of sepiolite carrier.
Increase in the amount of peroxidase leads to the increase in the specific activity of immobilized
enzyme. The highest activity was achieved when 15 mg of periodate-oxidized peroxidase was
immobilized per gram of sepiolite. Temperature and operational stabilities of immobilized
peroxidases were tested. Oxidized peroxidase retained 42% of initial activity after
4 consecutive cycles of pyrogallol oxidation.",
journal = "UNIVERZITET U BEOGRADU HEMIJSKI FAKULTET",
title = "Uklanjanje fenola i tekstilnih boja iz otpadnih voda imobilizovanim peroksidazama iz rena (Armoracia rusticana) i gljive bele truleži (Phanerochaete chrysosporium), Phenol and textile dye removal from wastewater with immobilized peroxidases from Horseradish (Armoracia rusticana) and white rot fungi (Phanerochaete chrysosporium)",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3214"
}
Surudžić, N.. (2024). Uklanjanje fenola i tekstilnih boja iz otpadnih voda imobilizovanim peroksidazama iz rena (Armoracia rusticana) i gljive bele truleži (Phanerochaete chrysosporium). in UNIVERZITET U BEOGRADU HEMIJSKI FAKULTET.
https://hdl.handle.net/21.15107/rcub_rimsi_3214
Surudžić N. Uklanjanje fenola i tekstilnih boja iz otpadnih voda imobilizovanim peroksidazama iz rena (Armoracia rusticana) i gljive bele truleži (Phanerochaete chrysosporium). in UNIVERZITET U BEOGRADU HEMIJSKI FAKULTET. 2024;.
https://hdl.handle.net/21.15107/rcub_rimsi_3214 .
Surudžić, Nevena, "Uklanjanje fenola i tekstilnih boja iz otpadnih voda imobilizovanim peroksidazama iz rena (Armoracia rusticana) i gljive bele truleži (Phanerochaete chrysosporium)" in UNIVERZITET U BEOGRADU HEMIJSKI FAKULTET (2024),
https://hdl.handle.net/21.15107/rcub_rimsi_3214 .

In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

Pajic, Tanja; Stevanović, Katarina; Todorović, Nataša; Krmpot, Aleksandar J.; Živić, Miroslav; Savić-Šević, Svetlana; Lević, Steva M.; Stanić, Marina; Pantelić, Dejan; Jelenković, Brana; Rabasović, Mihailo

(Springer Nature, 2024)

TY  - JOUR
AU  - Pajic, Tanja
AU  - Stevanović, Katarina
AU  - Todorović, Nataša
AU  - Krmpot, Aleksandar J.
AU  - Živić, Miroslav
AU  - Savić-Šević, Svetlana
AU  - Lević, Steva M.
AU  - Stanić, Marina
AU  - Pantelić, Dejan
AU  - Jelenković, Brana
AU  - Rabasović, Mihailo
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3213
AB  - Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens. However, their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell wall. Here, we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi, which enabled patch-clamp measurements on protoplasts released from hyphae. A reproducible and highly precise (diffraction-limited, submicron resolution) method for obtaining viable released protoplasts was developed. Protoplast release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the hyphae. The plasma membrane of the obtained protoplasts formed tight and high-resistance (GΩ) contacts with the recording pipette. The entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 hour. Compared to previous studies using heterologously expressed channels, this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.
PB  - Springer Nature
T2  - Microsystems & Nanoengineering
T1  - In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi
SP  - 47
VL  - 10
DO  - 10.1038/s41378-024-00664-x
ER  - 
@article{
author = "Pajic, Tanja and Stevanović, Katarina and Todorović, Nataša and Krmpot, Aleksandar J. and Živić, Miroslav and Savić-Šević, Svetlana and Lević, Steva M. and Stanić, Marina and Pantelić, Dejan and Jelenković, Brana and Rabasović, Mihailo",
year = "2024",
abstract = "Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens. However, their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell wall. Here, we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi, which enabled patch-clamp measurements on protoplasts released from hyphae. A reproducible and highly precise (diffraction-limited, submicron resolution) method for obtaining viable released protoplasts was developed. Protoplast release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the hyphae. The plasma membrane of the obtained protoplasts formed tight and high-resistance (GΩ) contacts with the recording pipette. The entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 hour. Compared to previous studies using heterologously expressed channels, this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.",
publisher = "Springer Nature",
journal = "Microsystems & Nanoengineering",
title = "In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi",
pages = "47",
volume = "10",
doi = "10.1038/s41378-024-00664-x"
}
Pajic, T., Stevanović, K., Todorović, N., Krmpot, A. J., Živić, M., Savić-Šević, S., Lević, S. M., Stanić, M., Pantelić, D., Jelenković, B.,& Rabasović, M.. (2024). In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi. in Microsystems & Nanoengineering
Springer Nature., 10, 47.
https://doi.org/10.1038/s41378-024-00664-x
Pajic T, Stevanović K, Todorović N, Krmpot AJ, Živić M, Savić-Šević S, Lević SM, Stanić M, Pantelić D, Jelenković B, Rabasović M. In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi. in Microsystems & Nanoengineering. 2024;10:47.
doi:10.1038/s41378-024-00664-x .
Pajic, Tanja, Stevanović, Katarina, Todorović, Nataša, Krmpot, Aleksandar J., Živić, Miroslav, Savić-Šević, Svetlana, Lević, Steva M., Stanić, Marina, Pantelić, Dejan, Jelenković, Brana, Rabasović, Mihailo, "In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi" in Microsystems & Nanoengineering, 10 (2024):47,
https://doi.org/10.1038/s41378-024-00664-x . .

Advantages and limitations of active phase silanization in PVDF composites: Focus on electrical properties and energy harvesting potential

Vijatović Petrović, Mirjana; Craciun, Floriana; Cordero, Francesco; MERCADELLI, ELISA; Ilić, Nikola; Despotovic, Zeljko V.; Bobić, Jelena; Džunuzović, Adis; Galassi, Carmen; Stagnaro, Paola; Canu, Giovanna; Buscaglia, Maria Teresa; Brunengo, Elisabetta

(John Wiley and Sons Inc, 2024)

TY  - JOUR
AU  - Vijatović Petrović, Mirjana
AU  - Craciun, Floriana
AU  - Cordero, Francesco
AU  - MERCADELLI, ELISA
AU  - Ilić, Nikola
AU  - Despotovic, Zeljko V.
AU  - Bobić, Jelena
AU  - Džunuzović, Adis
AU  - Galassi, Carmen
AU  - Stagnaro, Paola
AU  - Canu, Giovanna
AU  - Buscaglia, Maria Teresa
AU  - Brunengo, Elisabetta
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3211
AB  - In order to further improve the performance of 0.94[(Bi0.5Na0.5)TiO3]-0.06BaTiO3/
polyvinylidene fluoride (NBT-BT/PVDF) flexible composite films prepared by the
hot-pressing method, the effect of surface modification of the NBT-BT particles on
the structure and properties of the films was investigated. Two coupling agents,
namely, (3-aminopropyl)triethoxysilane (APTES) and dodecyl triethoxysilane
(DDTES) were added to enhance dispersion and interfacial adhesion of the active
phase powder with the polymer matrix. The highest amount of the electroactive
PVDF β-phase was formed in APTES-modified samples while in DDTES samples
mainly γ-phase was formed as shown by Fourier-transform infrared spectroscopy
analysis. Differential scanning calorimetry measurements indicated that the addition
of filler particles reduced the total crystallinity degree of the PVDF. Dielectric
permittivity values as well as dielectric losses decreased for silanized samples due
to reduced tension at the interface between particles and polymer. Strong intermolecular
interaction between the PVDF chains and the APTES-modified particles
led to enhanced breakdown strength of these samples. The highest level of
agglomeration in the DDTES-modified samples induced the deterioration of ferroelectric
properties. The highest voltage output of  15 V and 225 μW of powerwas
obtained for the APTES-modified harvester, evidencing their potential for energy
harvesting applications.
PB  - John Wiley and Sons Inc
T2  - Polymer Composites
T1  - Advantages and limitations of active phase silanization in PVDF composites: Focus on electrical properties and energy harvesting potential
EP  - 4446
IS  - 5
SP  - 4428
VL  - 45
DO  - 10.1002/pc.28071
ER  - 
@article{
author = "Vijatović Petrović, Mirjana and Craciun, Floriana and Cordero, Francesco and MERCADELLI, ELISA and Ilić, Nikola and Despotovic, Zeljko V. and Bobić, Jelena and Džunuzović, Adis and Galassi, Carmen and Stagnaro, Paola and Canu, Giovanna and Buscaglia, Maria Teresa and Brunengo, Elisabetta",
year = "2024",
abstract = "In order to further improve the performance of 0.94[(Bi0.5Na0.5)TiO3]-0.06BaTiO3/
polyvinylidene fluoride (NBT-BT/PVDF) flexible composite films prepared by the
hot-pressing method, the effect of surface modification of the NBT-BT particles on
the structure and properties of the films was investigated. Two coupling agents,
namely, (3-aminopropyl)triethoxysilane (APTES) and dodecyl triethoxysilane
(DDTES) were added to enhance dispersion and interfacial adhesion of the active
phase powder with the polymer matrix. The highest amount of the electroactive
PVDF β-phase was formed in APTES-modified samples while in DDTES samples
mainly γ-phase was formed as shown by Fourier-transform infrared spectroscopy
analysis. Differential scanning calorimetry measurements indicated that the addition
of filler particles reduced the total crystallinity degree of the PVDF. Dielectric
permittivity values as well as dielectric losses decreased for silanized samples due
to reduced tension at the interface between particles and polymer. Strong intermolecular
interaction between the PVDF chains and the APTES-modified particles
led to enhanced breakdown strength of these samples. The highest level of
agglomeration in the DDTES-modified samples induced the deterioration of ferroelectric
properties. The highest voltage output of  15 V and 225 μW of powerwas
obtained for the APTES-modified harvester, evidencing their potential for energy
harvesting applications.",
publisher = "John Wiley and Sons Inc",
journal = "Polymer Composites",
title = "Advantages and limitations of active phase silanization in PVDF composites: Focus on electrical properties and energy harvesting potential",
pages = "4446-4428",
number = "5",
volume = "45",
doi = "10.1002/pc.28071"
}
Vijatović Petrović, M., Craciun, F., Cordero, F., MERCADELLI, E., Ilić, N., Despotovic, Z. V., Bobić, J., Džunuzović, A., Galassi, C., Stagnaro, P., Canu, G., Buscaglia, M. T.,& Brunengo, E.. (2024). Advantages and limitations of active phase silanization in PVDF composites: Focus on electrical properties and energy harvesting potential. in Polymer Composites
John Wiley and Sons Inc., 45(5), 4428-4446.
https://doi.org/10.1002/pc.28071
Vijatović Petrović M, Craciun F, Cordero F, MERCADELLI E, Ilić N, Despotovic ZV, Bobić J, Džunuzović A, Galassi C, Stagnaro P, Canu G, Buscaglia MT, Brunengo E. Advantages and limitations of active phase silanization in PVDF composites: Focus on electrical properties and energy harvesting potential. in Polymer Composites. 2024;45(5):4428-4446.
doi:10.1002/pc.28071 .
Vijatović Petrović, Mirjana, Craciun, Floriana, Cordero, Francesco, MERCADELLI, ELISA, Ilić, Nikola, Despotovic, Zeljko V., Bobić, Jelena, Džunuzović, Adis, Galassi, Carmen, Stagnaro, Paola, Canu, Giovanna, Buscaglia, Maria Teresa, Brunengo, Elisabetta, "Advantages and limitations of active phase silanization in PVDF composites: Focus on electrical properties and energy harvesting potential" in Polymer Composites, 45, no. 5 (2024):4428-4446,
https://doi.org/10.1002/pc.28071 . .
2
2

Variability of Morpho-Anatomical Characteristics of Different Willow Clones Contaminated with Heavy Metals

Jokanović, Dušan; Urošević, Jelena; Stojnić, Srđan; Nikolić Jokanović, Vesna; Stanković, Dragica; Ištok, Iva

(Crojfe, 2024)

TY  - JOUR
AU  - Jokanović, Dušan
AU  - Urošević, Jelena
AU  - Stojnić, Srđan
AU  - Nikolić Jokanović, Vesna
AU  - Stanković, Dragica
AU  - Ištok, Iva
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3212
AB  - In this paper, the variability of morphological (stem height, stem basal diameter, proportion of pith, wood and bark) and wood anatomical characteristics (fiber length, fiber diameter, fiber lumen diamater, double cell-wall thickness, vessel diameter, wood rays width and height) of three Salix alba clones (B-44, 347 and NS 73/6) and one Salix viminalis clone both in the control plot and in the site contaminated with a mixture of heavy metals (As, Cd, Cr, Cu, Ni, Pb) was investigated. The observed results showed that individuals of all four clones had significantly higher average values of stem height and stem basal diameter at the control plot compared to the polluted site. As for the proportion of pith, bark and wood, heavy metals caused an increase in the share of pith and a decrease in the share of bark and wood in all clones with the exception of clone NS 73/6. The analysis of wood fiber dimensions showed that the values of all parameters were higher at the control site with the exception of fiber lumen diameter where higher values were observed for clones B-44 and NS 73/6 at the polluted site. Higher values of vessel diameter were recorded for all clones at the control plot, while wood rays width of all individuals was greater at the contaminated site. Regarding the wood rays height, only Salix viminalis showed higher value at the polluted site. These results confirmed that pollution-induced heavy metal stress significantly altered the morphological and wood anatomical characteristics of all researched clones and that it may affect their utility properties.
PB  - Crojfe
T2  - Croatian Journal of Forest Engineering
T1  - Variability of Morpho-Anatomical Characteristics of Different Willow Clones Contaminated with Heavy Metals
VL  - 45
DO  - 10.5552/crojfe.2024.2289
ER  - 
@article{
author = "Jokanović, Dušan and Urošević, Jelena and Stojnić, Srđan and Nikolić Jokanović, Vesna and Stanković, Dragica and Ištok, Iva",
year = "2024",
abstract = "In this paper, the variability of morphological (stem height, stem basal diameter, proportion of pith, wood and bark) and wood anatomical characteristics (fiber length, fiber diameter, fiber lumen diamater, double cell-wall thickness, vessel diameter, wood rays width and height) of three Salix alba clones (B-44, 347 and NS 73/6) and one Salix viminalis clone both in the control plot and in the site contaminated with a mixture of heavy metals (As, Cd, Cr, Cu, Ni, Pb) was investigated. The observed results showed that individuals of all four clones had significantly higher average values of stem height and stem basal diameter at the control plot compared to the polluted site. As for the proportion of pith, bark and wood, heavy metals caused an increase in the share of pith and a decrease in the share of bark and wood in all clones with the exception of clone NS 73/6. The analysis of wood fiber dimensions showed that the values of all parameters were higher at the control site with the exception of fiber lumen diameter where higher values were observed for clones B-44 and NS 73/6 at the polluted site. Higher values of vessel diameter were recorded for all clones at the control plot, while wood rays width of all individuals was greater at the contaminated site. Regarding the wood rays height, only Salix viminalis showed higher value at the polluted site. These results confirmed that pollution-induced heavy metal stress significantly altered the morphological and wood anatomical characteristics of all researched clones and that it may affect their utility properties.",
publisher = "Crojfe",
journal = "Croatian Journal of Forest Engineering",
title = "Variability of Morpho-Anatomical Characteristics of Different Willow Clones Contaminated with Heavy Metals",
volume = "45",
doi = "10.5552/crojfe.2024.2289"
}
Jokanović, D., Urošević, J., Stojnić, S., Nikolić Jokanović, V., Stanković, D.,& Ištok, I.. (2024). Variability of Morpho-Anatomical Characteristics of Different Willow Clones Contaminated with Heavy Metals. in Croatian Journal of Forest Engineering
Crojfe., 45.
https://doi.org/10.5552/crojfe.2024.2289
Jokanović D, Urošević J, Stojnić S, Nikolić Jokanović V, Stanković D, Ištok I. Variability of Morpho-Anatomical Characteristics of Different Willow Clones Contaminated with Heavy Metals. in Croatian Journal of Forest Engineering. 2024;45.
doi:10.5552/crojfe.2024.2289 .
Jokanović, Dušan, Urošević, Jelena, Stojnić, Srđan, Nikolić Jokanović, Vesna, Stanković, Dragica, Ištok, Iva, "Variability of Morpho-Anatomical Characteristics of Different Willow Clones Contaminated with Heavy Metals" in Croatian Journal of Forest Engineering, 45 (2024),
https://doi.org/10.5552/crojfe.2024.2289 . .

Looking Into How Nickel Doping Affects the Structure, Morphology, and Optical Properties of TiO2 Nanofibers

Ahmetović, Sanita; Vasiljević, Zorka Z; Krstić, Jugoslav B.; Finšgar, Matjaž; Solonenko, Dmytro; Bartolić, Dragana; Tadić, Nenad B.; Mišković, Goran; Cvjetićanin, Nikola; Nikolić, Maria Vesna

(Elsevier, 2024)

TY  - JOUR
AU  - Ahmetović, Sanita
AU  - Vasiljević, Zorka Z
AU  - Krstić, Jugoslav B.
AU  - Finšgar, Matjaž
AU  - Solonenko, Dmytro
AU  - Bartolić, Dragana
AU  - Tadić, Nenad B.
AU  - Mišković, Goran
AU  - Cvjetićanin, Nikola
AU  - Nikolić, Maria Vesna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3204
AB  - In this paper, we have systematically studied the structural, morphological, and optical properties of Ni-doped TiO2, synthesized via a simple, cost-effective electrospinning method followed by calcination at 500 C. The nanofibers with a core-shell structure were relatively homogeneous, smooth and randomly oriented, and there were no significant differences in fiber diameters due to Ni2+ content. Core loss mapping using electron energy loss spectroscopy confirmed an even distribution of titanium and relatively uniform nickel in the fibers. It was found that doping with 0.5 mol.% Ni2+ decreased the rutile content, while doping with 1 mol.% Ni2+ resulted in a pure anatase phase with a significantly increased specific surface area (36.6 m2/g). Further increase in Ni2+ content (3-10 mol.%) not only prolonged the response of TiO2 nanofibers to visible light, but also increased the specific surface area (49.5 m2/g), decreased crystallite size (7 nm), and increased rutile content in TiO2 (33 wt.%). Photoluminescence analysis revealed that doping TiO2 with different amounts of Ni2+ leads to a gradual decrease of emission spectra intensity and red shift in the maxima positions. The XPS results confirmed that as the Ni2+ content enlarged, the Ti2+ and Ti3+ content increased significantly, effectively promoting the formation of oxygen vacancies. Raman analysis showed that an increase in nickel content (3-5 mol.%) led to a decrease and shift in peak intensity due to Ti3+ formation and also the possible presence of NiTiO3 phases. HRTEM analysis showed that Ni was doped into the substitution sites of both the anatase and rutile TiO2 lattice but had a stronger influence on the distortion of the anatase phase. The photocatalytic activity of Ni-doped TiO2 nanofibers was explored by analyzing the degradation of an antibiotic, oxytetracycline, monitored in laboratory conditions under visible light irradiation. After 60 minutes of irradiation, the degradation  of OTC with 1Ni-TiO2 reached 76.4% and with 10Ni-TiO2 70.5%.
PB  - Elsevier
T2  - Surfaces and Interfaces
T1  - Looking Into How Nickel Doping Affects the Structure, Morphology, and Optical Properties of TiO2 Nanofibers
SP  - 104434
VL  - 49
DO  - 10.1016/j.surfin.2024.104434
ER  - 
@article{
author = "Ahmetović, Sanita and Vasiljević, Zorka Z and Krstić, Jugoslav B. and Finšgar, Matjaž and Solonenko, Dmytro and Bartolić, Dragana and Tadić, Nenad B. and Mišković, Goran and Cvjetićanin, Nikola and Nikolić, Maria Vesna",
year = "2024",
abstract = "In this paper, we have systematically studied the structural, morphological, and optical properties of Ni-doped TiO2, synthesized via a simple, cost-effective electrospinning method followed by calcination at 500 C. The nanofibers with a core-shell structure were relatively homogeneous, smooth and randomly oriented, and there were no significant differences in fiber diameters due to Ni2+ content. Core loss mapping using electron energy loss spectroscopy confirmed an even distribution of titanium and relatively uniform nickel in the fibers. It was found that doping with 0.5 mol.% Ni2+ decreased the rutile content, while doping with 1 mol.% Ni2+ resulted in a pure anatase phase with a significantly increased specific surface area (36.6 m2/g). Further increase in Ni2+ content (3-10 mol.%) not only prolonged the response of TiO2 nanofibers to visible light, but also increased the specific surface area (49.5 m2/g), decreased crystallite size (7 nm), and increased rutile content in TiO2 (33 wt.%). Photoluminescence analysis revealed that doping TiO2 with different amounts of Ni2+ leads to a gradual decrease of emission spectra intensity and red shift in the maxima positions. The XPS results confirmed that as the Ni2+ content enlarged, the Ti2+ and Ti3+ content increased significantly, effectively promoting the formation of oxygen vacancies. Raman analysis showed that an increase in nickel content (3-5 mol.%) led to a decrease and shift in peak intensity due to Ti3+ formation and also the possible presence of NiTiO3 phases. HRTEM analysis showed that Ni was doped into the substitution sites of both the anatase and rutile TiO2 lattice but had a stronger influence on the distortion of the anatase phase. The photocatalytic activity of Ni-doped TiO2 nanofibers was explored by analyzing the degradation of an antibiotic, oxytetracycline, monitored in laboratory conditions under visible light irradiation. After 60 minutes of irradiation, the degradation  of OTC with 1Ni-TiO2 reached 76.4% and with 10Ni-TiO2 70.5%.",
publisher = "Elsevier",
journal = "Surfaces and Interfaces",
title = "Looking Into How Nickel Doping Affects the Structure, Morphology, and Optical Properties of TiO2 Nanofibers",
pages = "104434",
volume = "49",
doi = "10.1016/j.surfin.2024.104434"
}
Ahmetović, S., Vasiljević, Z. Z., Krstić, J. B., Finšgar, M., Solonenko, D., Bartolić, D., Tadić, N. B., Mišković, G., Cvjetićanin, N.,& Nikolić, M. V.. (2024). Looking Into How Nickel Doping Affects the Structure, Morphology, and Optical Properties of TiO2 Nanofibers. in Surfaces and Interfaces
Elsevier., 49, 104434.
https://doi.org/10.1016/j.surfin.2024.104434
Ahmetović S, Vasiljević ZZ, Krstić JB, Finšgar M, Solonenko D, Bartolić D, Tadić NB, Mišković G, Cvjetićanin N, Nikolić MV. Looking Into How Nickel Doping Affects the Structure, Morphology, and Optical Properties of TiO2 Nanofibers. in Surfaces and Interfaces. 2024;49:104434.
doi:10.1016/j.surfin.2024.104434 .
Ahmetović, Sanita, Vasiljević, Zorka Z, Krstić, Jugoslav B., Finšgar, Matjaž, Solonenko, Dmytro, Bartolić, Dragana, Tadić, Nenad B., Mišković, Goran, Cvjetićanin, Nikola, Nikolić, Maria Vesna, "Looking Into How Nickel Doping Affects the Structure, Morphology, and Optical Properties of TiO2 Nanofibers" in Surfaces and Interfaces, 49 (2024):104434,
https://doi.org/10.1016/j.surfin.2024.104434 . .

NTC thermistor ferrite composite for temperature sensing with reduced humidity influence

Nikolić, Maria Vesna; Vasiljević, Zorka Z; Dojčinović, Milena

(Czech Technical University in Prague, Faculty of Electrical Engineering, Prague, Czech Republic, 2024)

TY  - CONF
AU  - Nikolić, Maria Vesna
AU  - Vasiljević, Zorka Z
AU  - Dojčinović, Milena
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3210
AB  - Negative temperature coefficient (NTC) thermistor semiconducting oxides, such as ferrites used as resistance-based temperature sensors, remain in research focus, as they are basic building blocks of many electronic systems. In this work we have investigated NTC thermistor properties of a ferrite composite (MnFe2O4/Fe2O3) ceramics obtained by solid-state sintering in the form of a bulk sample with a diameter of 8.7 mm and thickness of 1.8 mm. We obtained a thermal (material) constant (B10,90) of 4390 K from measured impedance at 100 Hz in the temperature range 10 - 90 C and temperature sensitivity (alpha) of -4.87 %/K at 25 oC, that was within the range required for a commercial NTC thermistor material (B between 2000 and 5000,  between -2 and 6% K at room temperature -25 oC). The influence of relative humidity (RH) in the range 30-90% at 25 and 50 C on impedance in the frequency range 50 Hz – 1 MHz was monitored. The obtained results showed that at 100 Hz the change in impedance was from 8.15 to 3.56 Mohm at 25 oC and 2.81 to 1.65 Mohm for RH from 30 to 90%, indicating that the humidity influence was reduced compared to other NTC thermistor materials. Future work will focus on applying this ferrite composite in flexible printed temperature sensors.
PB  - Czech Technical University in Prague, Faculty of Electrical Engineering, Prague, Czech Republic
C3  - Conference program and extended abstracts of the 47th International Spring Seminar on Electronics Technology "Trends in Electronics Manufacturing, Interconection Technology, and Microelectronics Packaging", 15-19 May 2024, Prague, Czech Republic
T1  - NTC thermistor ferrite composite for temperature sensing with reduced humidity influence
EP  - 25
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3210
ER  - 
@conference{
author = "Nikolić, Maria Vesna and Vasiljević, Zorka Z and Dojčinović, Milena",
year = "2024",
abstract = "Negative temperature coefficient (NTC) thermistor semiconducting oxides, such as ferrites used as resistance-based temperature sensors, remain in research focus, as they are basic building blocks of many electronic systems. In this work we have investigated NTC thermistor properties of a ferrite composite (MnFe2O4/Fe2O3) ceramics obtained by solid-state sintering in the form of a bulk sample with a diameter of 8.7 mm and thickness of 1.8 mm. We obtained a thermal (material) constant (B10,90) of 4390 K from measured impedance at 100 Hz in the temperature range 10 - 90 C and temperature sensitivity (alpha) of -4.87 %/K at 25 oC, that was within the range required for a commercial NTC thermistor material (B between 2000 and 5000,  between -2 and 6% K at room temperature -25 oC). The influence of relative humidity (RH) in the range 30-90% at 25 and 50 C on impedance in the frequency range 50 Hz – 1 MHz was monitored. The obtained results showed that at 100 Hz the change in impedance was from 8.15 to 3.56 Mohm at 25 oC and 2.81 to 1.65 Mohm for RH from 30 to 90%, indicating that the humidity influence was reduced compared to other NTC thermistor materials. Future work will focus on applying this ferrite composite in flexible printed temperature sensors.",
publisher = "Czech Technical University in Prague, Faculty of Electrical Engineering, Prague, Czech Republic",
journal = "Conference program and extended abstracts of the 47th International Spring Seminar on Electronics Technology "Trends in Electronics Manufacturing, Interconection Technology, and Microelectronics Packaging", 15-19 May 2024, Prague, Czech Republic",
title = "NTC thermistor ferrite composite for temperature sensing with reduced humidity influence",
pages = "25-24",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3210"
}
Nikolić, M. V., Vasiljević, Z. Z.,& Dojčinović, M.. (2024). NTC thermistor ferrite composite for temperature sensing with reduced humidity influence. in Conference program and extended abstracts of the 47th International Spring Seminar on Electronics Technology "Trends in Electronics Manufacturing, Interconection Technology, and Microelectronics Packaging", 15-19 May 2024, Prague, Czech Republic
Czech Technical University in Prague, Faculty of Electrical Engineering, Prague, Czech Republic., 24-25.
https://hdl.handle.net/21.15107/rcub_rimsi_3210
Nikolić MV, Vasiljević ZZ, Dojčinović M. NTC thermistor ferrite composite for temperature sensing with reduced humidity influence. in Conference program and extended abstracts of the 47th International Spring Seminar on Electronics Technology "Trends in Electronics Manufacturing, Interconection Technology, and Microelectronics Packaging", 15-19 May 2024, Prague, Czech Republic. 2024;:24-25.
https://hdl.handle.net/21.15107/rcub_rimsi_3210 .
Nikolić, Maria Vesna, Vasiljević, Zorka Z, Dojčinović, Milena, "NTC thermistor ferrite composite for temperature sensing with reduced humidity influence" in Conference program and extended abstracts of the 47th International Spring Seminar on Electronics Technology "Trends in Electronics Manufacturing, Interconection Technology, and Microelectronics Packaging", 15-19 May 2024, Prague, Czech Republic (2024):24-25,
https://hdl.handle.net/21.15107/rcub_rimsi_3210 .

An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity

Vasiljević, Zorka Z; Vunduk, Jovana; Bartolić, Dragana; Miskovic, Goran; Ognjanović, Miloš; Tadic, Nenad; Nikolić, Maria Vesna

(American Chemical Society, 2024)

TY  - JOUR
AU  - Vasiljević, Zorka Z
AU  - Vunduk, Jovana
AU  - Bartolić, Dragana
AU  - Miskovic, Goran
AU  - Ognjanović, Miloš
AU  - Tadic, Nenad
AU  - Nikolić, Maria Vesna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3209
AB  - Emission  of greenhouse gases and infectious diseases caused by improper agro-waste disposal has gained significant attention in recent years. To overcome these hurdles, agro-waste can be valorized into valuable bioactive compounds that act as reducing or stabilizing agents in the synthesis of nanomaterials. Herein, we report a simple circular approach using Citrus reticulata Blanco (C. reticulata) waste (peel powder/aqueous extract) as green reducing and capping/stabilizing agents and Zn nitrate/acetate precursors to synthesize ZnO nanoparticles (NPs) with efficient antimicrobial and photocatalytic activities. The obtained NPs crystallized in a hexagonal wurtzite structure and differed clearly in their morphology. UV-vis analysis of the nanoparticles showed a characteristic broad absorption band between 330 and 414 nm, belonging to ZnO NPs. Fourier transform infrared (FTIR) spectroscopy of ZnO NPs exhibited a Zn–O band close to 450 cm−1. The band gap values were in the range of 2.84-3.14 eV depending on the precursor and agent used. The crystallite size obtained from size-strain plots from measured XRD patterns was between 7 and 26 nm, with the strain between 16 and 4%.  The high crystalline nature of obtained ZnO NPs was confirmed by clear ring diffraction patterns and d-spacing values of the observed lattice fringes. ZnNPeelMan_400 and ZnNExtrMan have shown good stability as the Zeta potential was found to be around -20 mV and reduced particle aggregation. Photoluminescence analysis revealed different defects belonging to oxygen vacancies (VO+ and VO++) and zinc interstitial (Zni) sites. The presence of oxygen vacancies on the surface of ZnAcExtrMan_400 and ZnAcPeelMan_400 increased antimicrobial activity, specifically against gram-negative bacteria Escherichia coli (E.coli) and Salmonella enteritidis (S. enteritidis). ZnNextrMan with a minimal inhibitory concentration of 0.156 mg/ml was more effective against gram-positive bacteria, Staphylococcus aureus (S. aureus) revealing a high influence of particle size and shape on antimicrobial activity. In addition, the photocatalytic activity of the ZnO NPs was examined by assessing the degradation of acid green dye in an aqueous solution under UV light irradiation. ZnAcPeelMan_400 exhibited excellent photocatalytic activity (94%) within 90 minutes after irradiation compared to other obtained ZnO NPs.
PB  - American Chemical Society
T2  - ACS Applied Bio Materials
T1  - An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity
EP  - 3032
SP  - 3014
VL  - 7
DO  - 10.1021/acsabm.4c00079
ER  - 
@article{
author = "Vasiljević, Zorka Z and Vunduk, Jovana and Bartolić, Dragana and Miskovic, Goran and Ognjanović, Miloš and Tadic, Nenad and Nikolić, Maria Vesna",
year = "2024",
abstract = "Emission  of greenhouse gases and infectious diseases caused by improper agro-waste disposal has gained significant attention in recent years. To overcome these hurdles, agro-waste can be valorized into valuable bioactive compounds that act as reducing or stabilizing agents in the synthesis of nanomaterials. Herein, we report a simple circular approach using Citrus reticulata Blanco (C. reticulata) waste (peel powder/aqueous extract) as green reducing and capping/stabilizing agents and Zn nitrate/acetate precursors to synthesize ZnO nanoparticles (NPs) with efficient antimicrobial and photocatalytic activities. The obtained NPs crystallized in a hexagonal wurtzite structure and differed clearly in their morphology. UV-vis analysis of the nanoparticles showed a characteristic broad absorption band between 330 and 414 nm, belonging to ZnO NPs. Fourier transform infrared (FTIR) spectroscopy of ZnO NPs exhibited a Zn–O band close to 450 cm−1. The band gap values were in the range of 2.84-3.14 eV depending on the precursor and agent used. The crystallite size obtained from size-strain plots from measured XRD patterns was between 7 and 26 nm, with the strain between 16 and 4%.  The high crystalline nature of obtained ZnO NPs was confirmed by clear ring diffraction patterns and d-spacing values of the observed lattice fringes. ZnNPeelMan_400 and ZnNExtrMan have shown good stability as the Zeta potential was found to be around -20 mV and reduced particle aggregation. Photoluminescence analysis revealed different defects belonging to oxygen vacancies (VO+ and VO++) and zinc interstitial (Zni) sites. The presence of oxygen vacancies on the surface of ZnAcExtrMan_400 and ZnAcPeelMan_400 increased antimicrobial activity, specifically against gram-negative bacteria Escherichia coli (E.coli) and Salmonella enteritidis (S. enteritidis). ZnNextrMan with a minimal inhibitory concentration of 0.156 mg/ml was more effective against gram-positive bacteria, Staphylococcus aureus (S. aureus) revealing a high influence of particle size and shape on antimicrobial activity. In addition, the photocatalytic activity of the ZnO NPs was examined by assessing the degradation of acid green dye in an aqueous solution under UV light irradiation. ZnAcPeelMan_400 exhibited excellent photocatalytic activity (94%) within 90 minutes after irradiation compared to other obtained ZnO NPs.",
publisher = "American Chemical Society",
journal = "ACS Applied Bio Materials",
title = "An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity",
pages = "3032-3014",
volume = "7",
doi = "10.1021/acsabm.4c00079"
}
Vasiljević, Z. Z., Vunduk, J., Bartolić, D., Miskovic, G., Ognjanović, M., Tadic, N.,& Nikolić, M. V.. (2024). An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity. in ACS Applied Bio Materials
American Chemical Society., 7, 3014-3032.
https://doi.org/10.1021/acsabm.4c00079
Vasiljević ZZ, Vunduk J, Bartolić D, Miskovic G, Ognjanović M, Tadic N, Nikolić MV. An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity. in ACS Applied Bio Materials. 2024;7:3014-3032.
doi:10.1021/acsabm.4c00079 .
Vasiljević, Zorka Z, Vunduk, Jovana, Bartolić, Dragana, Miskovic, Goran, Ognjanović, Miloš, Tadic, Nenad, Nikolić, Maria Vesna, "An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity" in ACS Applied Bio Materials, 7 (2024):3014-3032,
https://doi.org/10.1021/acsabm.4c00079 . .

Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap

Ilijin, Larisa; Nikolić, Maria Vesna; Vasiljević, Zorka Z; Todorović, Dajana; Mrdaković, Marija; Vlahović, Milena; Matić, Dragana; Tadic, Nenad; Perić-Mataruga, Vesna

(Elsevier, 2024)

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Nikolić, Maria Vesna
AU  - Vasiljević, Zorka Z
AU  - Todorović, Dajana
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Tadic, Nenad
AU  - Perić-Mataruga, Vesna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3202
AB  - In this work we have characterized and compared chitin sourced from exoskeleton of Tenebrio molitor larvae fed with polystyrene or plastic kitchen wrap combined with bran in the ratio 1:1 with chitin sourced from larvae exoskeleton fed only with bran. Analysis of the frass by ATR-FTIR showed very similar spectra and confirmed degradation of the plastic feed components, while ATR-FTIR analysis of the exoskeleton verified the absence of any plastic residue. Deproteinization followed by demineralization produced 6.78 -5.29 % chitin, showing that plastic (polystyrene or plastic kitchen wrap) in the larvae diet resulted in heavier insect exoskeleton, but yielded slightly less chitin, with the lowest value obtained for plastic kitchen wrap in the insect diet. The deacetylation degree of 98.17-98.61% was determined from measured ATR-FTIR spectra. XRD analysis confirmed the presence of -chitin with a crystallinity index of 66.5-62% and crystallite size 4-5 nm. Thermogravimetric analysis showed similar degradation curves for all chitin samples, with two degradation steps. These results show that chitin sourced from exoskeleton of T. molitor larvae fed with plastic (polystyrene or plastic kitchen wrap) and contributing to significant biodegradation of major polluting materials can be a feasible and alternative source of chitin, further promoting a bio-circular economy.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap
SP  - 131731
VL  - 268
DO  - 10.1016/j.ijbiomac.2024.131731
ER  - 
@article{
author = "Ilijin, Larisa and Nikolić, Maria Vesna and Vasiljević, Zorka Z and Todorović, Dajana and Mrdaković, Marija and Vlahović, Milena and Matić, Dragana and Tadic, Nenad and Perić-Mataruga, Vesna",
year = "2024",
abstract = "In this work we have characterized and compared chitin sourced from exoskeleton of Tenebrio molitor larvae fed with polystyrene or plastic kitchen wrap combined with bran in the ratio 1:1 with chitin sourced from larvae exoskeleton fed only with bran. Analysis of the frass by ATR-FTIR showed very similar spectra and confirmed degradation of the plastic feed components, while ATR-FTIR analysis of the exoskeleton verified the absence of any plastic residue. Deproteinization followed by demineralization produced 6.78 -5.29 % chitin, showing that plastic (polystyrene or plastic kitchen wrap) in the larvae diet resulted in heavier insect exoskeleton, but yielded slightly less chitin, with the lowest value obtained for plastic kitchen wrap in the insect diet. The deacetylation degree of 98.17-98.61% was determined from measured ATR-FTIR spectra. XRD analysis confirmed the presence of -chitin with a crystallinity index of 66.5-62% and crystallite size 4-5 nm. Thermogravimetric analysis showed similar degradation curves for all chitin samples, with two degradation steps. These results show that chitin sourced from exoskeleton of T. molitor larvae fed with plastic (polystyrene or plastic kitchen wrap) and contributing to significant biodegradation of major polluting materials can be a feasible and alternative source of chitin, further promoting a bio-circular economy.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap",
pages = "131731",
volume = "268",
doi = "10.1016/j.ijbiomac.2024.131731"
}
Ilijin, L., Nikolić, M. V., Vasiljević, Z. Z., Todorović, D., Mrdaković, M., Vlahović, M., Matić, D., Tadic, N.,& Perić-Mataruga, V.. (2024). Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap. in International Journal of Biological Macromolecules
Elsevier., 268, 131731.
https://doi.org/10.1016/j.ijbiomac.2024.131731
Ilijin L, Nikolić MV, Vasiljević ZZ, Todorović D, Mrdaković M, Vlahović M, Matić D, Tadic N, Perić-Mataruga V. Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap. in International Journal of Biological Macromolecules. 2024;268:131731.
doi:10.1016/j.ijbiomac.2024.131731 .
Ilijin, Larisa, Nikolić, Maria Vesna, Vasiljević, Zorka Z, Todorović, Dajana, Mrdaković, Marija, Vlahović, Milena, Matić, Dragana, Tadic, Nenad, Perić-Mataruga, Vesna, "Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap" in International Journal of Biological Macromolecules, 268 (2024):131731,
https://doi.org/10.1016/j.ijbiomac.2024.131731 . .

ZnO and Fe2TiO5 Nanoparticles Obtained by Green Synthesis as Active Components of Alginate Food Packaging Films

Vasiljević, Zorka Z; Vunduk, Jovana; Dojčinović, Milena; Mišković, Goran; Tadic, Nenad; Vidic, Jasmina; Nikolić, Maria Vesna

(Elsevier, 2024)

TY  - JOUR
AU  - Vasiljević, Zorka Z
AU  - Vunduk, Jovana
AU  - Dojčinović, Milena
AU  - Mišković, Goran
AU  - Tadic, Nenad
AU  - Vidic, Jasmina
AU  - Nikolić, Maria Vesna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3190
AB  - In this paper, we have systematically studied the structural, morphological, and optical properties of Ni-doped TiO2, synthesized via a simple, cost-effective electrospinning method followed by calcination at 500 C. The nanofibers with a core-shell structure were relatively homogeneous, smooth and randomly oriented, and there were no significant differences in fiber diameters due to Ni2+ content. Core loss mapping using electron energy loss spectroscopy confirmed an even distribution of titanium and relatively uniform nickel in the fibers. It was found that doping with 0.5 mol.% Ni2+ decreased the rutile content, while doping with 1 mol.% Ni2+ resulted in a pure anatase phase with a significantly increased specific surface area (36.6 m2/g). Further increase in Ni2+ content (3-10 mol.%) not only prolonged the response of TiO2 nanofibers to visible light, but also increased the specific surface area (49.5 m2/g), decreased crystallite size (7 nm), and increased rutile content in TiO2 (33 wt.%). Photoluminescence analysis revealed that doping TiO2 with different amounts of Ni2+ leads to a gradual decrease of emission spectra intensity and red shift in the maxima positions. The XPS results confirmed that as the Ni2+ content enlarged, the Ti2+ and Ti3+ content increased significantly, effectively promoting the formation of oxygen vacancies. Raman analysis showed that an increase in nickel content (3-5 mol.%) led to a decrease and shift in peak intensity due to Ti3+ formation and also the possible presence of NiTiO3 phases. HRTEM analysis showed that Ni was doped into the substitution sites of both the anatase and rutile TiO2 lattice but had a stronger influence on the distortion of the anatase phase. The obtained results indicate that Ni-doped TiO2 nanofibers are good candidates for photocatalytic applications.
PB  - Elsevier
T2  - Food Packaging and Shelf Life
T1  - ZnO and Fe2TiO5 Nanoparticles Obtained by Green Synthesis as Active Components of Alginate Food Packaging Films
SP  - 101280
VL  - 43
DO  - 10.1016/j.fpsl.2024.101280
ER  - 
@article{
author = "Vasiljević, Zorka Z and Vunduk, Jovana and Dojčinović, Milena and Mišković, Goran and Tadic, Nenad and Vidic, Jasmina and Nikolić, Maria Vesna",
year = "2024",
abstract = "In this paper, we have systematically studied the structural, morphological, and optical properties of Ni-doped TiO2, synthesized via a simple, cost-effective electrospinning method followed by calcination at 500 C. The nanofibers with a core-shell structure were relatively homogeneous, smooth and randomly oriented, and there were no significant differences in fiber diameters due to Ni2+ content. Core loss mapping using electron energy loss spectroscopy confirmed an even distribution of titanium and relatively uniform nickel in the fibers. It was found that doping with 0.5 mol.% Ni2+ decreased the rutile content, while doping with 1 mol.% Ni2+ resulted in a pure anatase phase with a significantly increased specific surface area (36.6 m2/g). Further increase in Ni2+ content (3-10 mol.%) not only prolonged the response of TiO2 nanofibers to visible light, but also increased the specific surface area (49.5 m2/g), decreased crystallite size (7 nm), and increased rutile content in TiO2 (33 wt.%). Photoluminescence analysis revealed that doping TiO2 with different amounts of Ni2+ leads to a gradual decrease of emission spectra intensity and red shift in the maxima positions. The XPS results confirmed that as the Ni2+ content enlarged, the Ti2+ and Ti3+ content increased significantly, effectively promoting the formation of oxygen vacancies. Raman analysis showed that an increase in nickel content (3-5 mol.%) led to a decrease and shift in peak intensity due to Ti3+ formation and also the possible presence of NiTiO3 phases. HRTEM analysis showed that Ni was doped into the substitution sites of both the anatase and rutile TiO2 lattice but had a stronger influence on the distortion of the anatase phase. The obtained results indicate that Ni-doped TiO2 nanofibers are good candidates for photocatalytic applications.",
publisher = "Elsevier",
journal = "Food Packaging and Shelf Life",
title = "ZnO and Fe2TiO5 Nanoparticles Obtained by Green Synthesis as Active Components of Alginate Food Packaging Films",
pages = "101280",
volume = "43",
doi = "10.1016/j.fpsl.2024.101280"
}
Vasiljević, Z. Z., Vunduk, J., Dojčinović, M., Mišković, G., Tadic, N., Vidic, J.,& Nikolić, M. V.. (2024). ZnO and Fe2TiO5 Nanoparticles Obtained by Green Synthesis as Active Components of Alginate Food Packaging Films. in Food Packaging and Shelf Life
Elsevier., 43, 101280.
https://doi.org/10.1016/j.fpsl.2024.101280
Vasiljević ZZ, Vunduk J, Dojčinović M, Mišković G, Tadic N, Vidic J, Nikolić MV. ZnO and Fe2TiO5 Nanoparticles Obtained by Green Synthesis as Active Components of Alginate Food Packaging Films. in Food Packaging and Shelf Life. 2024;43:101280.
doi:10.1016/j.fpsl.2024.101280 .
Vasiljević, Zorka Z, Vunduk, Jovana, Dojčinović, Milena, Mišković, Goran, Tadic, Nenad, Vidic, Jasmina, Nikolić, Maria Vesna, "ZnO and Fe2TiO5 Nanoparticles Obtained by Green Synthesis as Active Components of Alginate Food Packaging Films" in Food Packaging and Shelf Life, 43 (2024):101280,
https://doi.org/10.1016/j.fpsl.2024.101280 . .

The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach

Djalović, Ivica; Mitrović, Petar; Trivan, Goran; Jelušić, Aleksandra; Pezo, Lato; Janić Hajnal, Elizabet; Popović Milovanović, Tatjana

(MDPI, 2024)

TY  - JOUR
AU  - Djalović, Ivica
AU  - Mitrović, Petar
AU  - Trivan, Goran
AU  - Jelušić, Aleksandra
AU  - Pezo, Lato
AU  - Janić Hajnal, Elizabet
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3208
AB  - Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to significant economic losses. The aim of this study was to determine biochemical changes in peony (Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca. Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation between different chemical parameters and revealed a clear separation among the samples. Based on the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP, NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced high r2 values (1.000 during the training period) and low SOS values. Developing an effective early warning system for the detection of plant diseases in different plant species is critical for improving crop yield and quality.
PB  - MDPI
T2  - Horticulturae
T1  - The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach
IS  - 5
SP  - 426
VL  - 10
DO  - 10.3390/horticulturae10050426
ER  - 
@article{
author = "Djalović, Ivica and Mitrović, Petar and Trivan, Goran and Jelušić, Aleksandra and Pezo, Lato and Janić Hajnal, Elizabet and Popović Milovanović, Tatjana",
year = "2024",
abstract = "Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to significant economic losses. The aim of this study was to determine biochemical changes in peony (Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca. Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation between different chemical parameters and revealed a clear separation among the samples. Based on the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP, NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced high r2 values (1.000 during the training period) and low SOS values. Developing an effective early warning system for the detection of plant diseases in different plant species is critical for improving crop yield and quality.",
publisher = "MDPI",
journal = "Horticulturae",
title = "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach",
number = "5",
pages = "426",
volume = "10",
doi = "10.3390/horticulturae10050426"
}
Djalović, I., Mitrović, P., Trivan, G., Jelušić, A., Pezo, L., Janić Hajnal, E.,& Popović Milovanović, T.. (2024). The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae
MDPI., 10(5), 426.
https://doi.org/10.3390/horticulturae10050426
Djalović I, Mitrović P, Trivan G, Jelušić A, Pezo L, Janić Hajnal E, Popović Milovanović T. The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae. 2024;10(5):426.
doi:10.3390/horticulturae10050426 .
Djalović, Ivica, Mitrović, Petar, Trivan, Goran, Jelušić, Aleksandra, Pezo, Lato, Janić Hajnal, Elizabet, Popović Milovanović, Tatjana, "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach" in Horticulturae, 10, no. 5 (2024):426,
https://doi.org/10.3390/horticulturae10050426 . .
1

Dietary supplementation with liophilised strawberry improves insulin sensitivity and redox status in mouse model of diet induced obesity

Brkljačić, Jelena; Jovanović, Mirna; Teofilović, Ana; Mićić, Bojana; Vratarić, Miloš; Milosavljević, Dragica; Dragišić Maksimović, Jelena; Maksimović, Vuk; Milivojević, Jasminka M; Djordjević, Ana

(Institute for Genetic Engineering and Biotechnology, University of Sarajevo, 2024)

TY  - CONF
AU  - Brkljačić, Jelena
AU  - Jovanović, Mirna
AU  - Teofilović, Ana
AU  - Mićić, Bojana
AU  - Vratarić, Miloš
AU  - Milosavljević, Dragica
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Milivojević, Jasminka M
AU  - Djordjević, Ana
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3207
AB  - Healthy dietary habits with abundant fruit consumption may reduce prevalence and positively affect development and progression of various chronic diseases including obesity and insulin-resistance related diseases. Strawberry (Fragaria × ananassa Duch.) represent a valuable source of vitamins, minerals, fatty acids, dietary fiber, and various bioactive polyphenolic compounds such as anthocyanins and flavonoids which are known for their antioxidant and anti-inflammatory activities. In this study we evaluated the effect of newly introduced strawberry cultivar „Aprika” supplemented in the form of lyophilized powder, on obesity-related metabolic alterations in high-fat-diet fed mice. We hypothesized that chronic (8 weeks) supplementation of lyophilized strawberries in the amount comparable to two servings per day in humans, would ameliorate insulin resistance associated with obesity in C57BL/6 mice. Furthermore, we hypothesized that strawberry meal consumed at 7PM i.e. before active feeding period of mice (which corresponds to early breakfast in humans) would induce more pronounced beneficial effects compared to meal consumed at 7AM i.e. at the end of the active feeding period (which corresponds to late dinner in humans). High fat diet induced hyperglycaemia, hyperinsulinaemia, insulin resistance and obesity; and disturbed hepatic insulin signaling. Lyophilized strawberries, only when consumed before feeding period, reduced body weight gain and improved insulin sensitivity induced by high-fat diet (evidenced by lower area under the curve after an intraperitoneal GTT, reduced serum insulin levels and an insulin resistance index (IR-HOMA). Strawberry meals consumed after active feeding period had no such effect. However, strawberries, regardless of the time of consumption, restored protein level of Insulin receptor substrate 1 (IRS1) in the liver and increased hepatic antioxidant enzymes level. In conclusion, strawberries improve insulin sensitivity and raise hepatic antioxidant capacity in mouse model of diet induced obesity. Nevertheless, more beneficial effects were achieved when strawberries were consumed before active feeding period, as an early breakfast
PB  - Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo. (Genetics and Applications; Special edition).
T1  - Dietary supplementation with liophilised strawberry improves insulin sensitivity and redox status in mouse model of diet induced obesity
EP  - 39
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3207
ER  - 
@conference{
author = "Brkljačić, Jelena and Jovanović, Mirna and Teofilović, Ana and Mićić, Bojana and Vratarić, Miloš and Milosavljević, Dragica and Dragišić Maksimović, Jelena and Maksimović, Vuk and Milivojević, Jasminka M and Djordjević, Ana",
year = "2024",
abstract = "Healthy dietary habits with abundant fruit consumption may reduce prevalence and positively affect development and progression of various chronic diseases including obesity and insulin-resistance related diseases. Strawberry (Fragaria × ananassa Duch.) represent a valuable source of vitamins, minerals, fatty acids, dietary fiber, and various bioactive polyphenolic compounds such as anthocyanins and flavonoids which are known for their antioxidant and anti-inflammatory activities. In this study we evaluated the effect of newly introduced strawberry cultivar „Aprika” supplemented in the form of lyophilized powder, on obesity-related metabolic alterations in high-fat-diet fed mice. We hypothesized that chronic (8 weeks) supplementation of lyophilized strawberries in the amount comparable to two servings per day in humans, would ameliorate insulin resistance associated with obesity in C57BL/6 mice. Furthermore, we hypothesized that strawberry meal consumed at 7PM i.e. before active feeding period of mice (which corresponds to early breakfast in humans) would induce more pronounced beneficial effects compared to meal consumed at 7AM i.e. at the end of the active feeding period (which corresponds to late dinner in humans). High fat diet induced hyperglycaemia, hyperinsulinaemia, insulin resistance and obesity; and disturbed hepatic insulin signaling. Lyophilized strawberries, only when consumed before feeding period, reduced body weight gain and improved insulin sensitivity induced by high-fat diet (evidenced by lower area under the curve after an intraperitoneal GTT, reduced serum insulin levels and an insulin resistance index (IR-HOMA). Strawberry meals consumed after active feeding period had no such effect. However, strawberries, regardless of the time of consumption, restored protein level of Insulin receptor substrate 1 (IRS1) in the liver and increased hepatic antioxidant enzymes level. In conclusion, strawberries improve insulin sensitivity and raise hepatic antioxidant capacity in mouse model of diet induced obesity. Nevertheless, more beneficial effects were achieved when strawberries were consumed before active feeding period, as an early breakfast",
publisher = "Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo. (Genetics and Applications; Special edition).",
title = "Dietary supplementation with liophilised strawberry improves insulin sensitivity and redox status in mouse model of diet induced obesity",
pages = "39-38",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3207"
}
Brkljačić, J., Jovanović, M., Teofilović, A., Mićić, B., Vratarić, M., Milosavljević, D., Dragišić Maksimović, J., Maksimović, V., Milivojević, J. M.,& Djordjević, A.. (2024). Dietary supplementation with liophilised strawberry improves insulin sensitivity and redox status in mouse model of diet induced obesity. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo. (Genetics and Applications; Special edition).
Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 38-39.
https://hdl.handle.net/21.15107/rcub_rimsi_3207
Brkljačić J, Jovanović M, Teofilović A, Mićić B, Vratarić M, Milosavljević D, Dragišić Maksimović J, Maksimović V, Milivojević JM, Djordjević A. Dietary supplementation with liophilised strawberry improves insulin sensitivity and redox status in mouse model of diet induced obesity. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo. (Genetics and Applications; Special edition).. 2024;:38-39.
https://hdl.handle.net/21.15107/rcub_rimsi_3207 .
Brkljačić, Jelena, Jovanović, Mirna, Teofilović, Ana, Mićić, Bojana, Vratarić, Miloš, Milosavljević, Dragica, Dragišić Maksimović, Jelena, Maksimović, Vuk, Milivojević, Jasminka M, Djordjević, Ana, "Dietary supplementation with liophilised strawberry improves insulin sensitivity and redox status in mouse model of diet induced obesity" in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo. (Genetics and Applications; Special edition). (2024):38-39,
https://hdl.handle.net/21.15107/rcub_rimsi_3207 .

HOW DOES SECTORIAL PRUNING AFFECT THE VEGETATIVE GROWTH, PRODUCTIVITY AND FRUIT QUALITY OF SUBSTRATE-GROWN HIGHBUSH BLUEBERRY CV. 'BLUECROP'?

Milivojević, Jasminka M; Radivojević, Dragan; Boskov, Djordje; Dragišić Maksimović, Jelena; Milosavljević, Dragica; Spasojević, Slavica; Veberič, Robert; Mikulič Petkovšek, Maja

(Strokovno sadjarsko društvo Slovenije, Jamnikarjeva 101, SI-1000 Ljubljana, 2024)

TY  - CONF
AU  - Milivojević, Jasminka M
AU  - Radivojević, Dragan
AU  - Boskov, Djordje
AU  - Dragišić Maksimović, Jelena
AU  - Milosavljević, Dragica
AU  - Spasojević, Slavica
AU  - Veberič, Robert
AU  - Mikulič Petkovšek, Maja
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3178
AB  - The impact of pruning techniques on the plant growth, yield components and fruit quality
traits of substrate-grown highbush blueberry (Vaccinium corymbosum) cultivar 'Bluecrop'
were evaluated over four years (2019-2022). The orchard was planted in the village of Gruža 
(Knić municipality, Serbia) in the spring of 2017 with 5-year-old nursery plants using 113 l 
polypropylene pots spaced 0.8 m apart in a row and 3.0 m between rows (4,170 plants/ha). At 
the onset of the trial the ‘Bluecrop’ plants were 8 years old. The two pruning techniques were 
applied: i) conventional pruning (CP) - removal of the most unproductive canes at the center
of the canopy, and weak and excessively fruiting shoots from the top of bushes, and ii) 
sectorial pruning (SP) - only removal of basal canes in ¼ of the canopy each year. The 
average number of basal canes per bush was significantly lower in SP than in the CP 
treatment (14.4 vs. 17.4 canes bush-1
, respectively). SP plants had thinner canes than CP 
plants, which increased over the years of the study and reached a maximum diameter of 11.12 
mm in 2022. CP had a positive influence on fruit yield (5.98 kg bush-1
), which was associated 
with a 20% higher number of fruits per bush than in sectorial pruned plants. The fruit yield 
increased in the first three years of the study, reaching a maximum value in 2021 (7.57 kg per 
bush), whereas an opposite trend was observed in average fruit weight over the years, with the 
lowest value reached in the last harvest year (1.81 g). Fruit weight and soluble solids content 
were not affected by the pruning method, while the content of total acids, total anthocyanins 
and total phenolics were significantly higher in fruit harvested from the SP treatment (0.70%, 
37.04 mg eq mvd-3-G /100 g FW and 1.18 mg eq GA/g FW, respectively) along with the total 
antioxidant capacity (8.39 mg eq AsA /g FW).
PB  - Strokovno sadjarsko društvo Slovenije, Jamnikarjeva 101, SI-1000 Ljubljana
C3  - 5. SLOVENSKEGA SADJARSKEGA KONGRESA Z MEDNARODNO UDELEŽBO
T1  - HOW DOES SECTORIAL PRUNING AFFECT THE  VEGETATIVE GROWTH, PRODUCTIVITY AND FRUIT  QUALITY OF SUBSTRATE-GROWN HIGHBUSH  BLUEBERRY CV. 'BLUECROP'?
SP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3178
ER  - 
@conference{
author = "Milivojević, Jasminka M and Radivojević, Dragan and Boskov, Djordje and Dragišić Maksimović, Jelena and Milosavljević, Dragica and Spasojević, Slavica and Veberič, Robert and Mikulič Petkovšek, Maja",
year = "2024",
abstract = "The impact of pruning techniques on the plant growth, yield components and fruit quality
traits of substrate-grown highbush blueberry (Vaccinium corymbosum) cultivar 'Bluecrop'
were evaluated over four years (2019-2022). The orchard was planted in the village of Gruža 
(Knić municipality, Serbia) in the spring of 2017 with 5-year-old nursery plants using 113 l 
polypropylene pots spaced 0.8 m apart in a row and 3.0 m between rows (4,170 plants/ha). At 
the onset of the trial the ‘Bluecrop’ plants were 8 years old. The two pruning techniques were 
applied: i) conventional pruning (CP) - removal of the most unproductive canes at the center
of the canopy, and weak and excessively fruiting shoots from the top of bushes, and ii) 
sectorial pruning (SP) - only removal of basal canes in ¼ of the canopy each year. The 
average number of basal canes per bush was significantly lower in SP than in the CP 
treatment (14.4 vs. 17.4 canes bush-1
, respectively). SP plants had thinner canes than CP 
plants, which increased over the years of the study and reached a maximum diameter of 11.12 
mm in 2022. CP had a positive influence on fruit yield (5.98 kg bush-1
), which was associated 
with a 20% higher number of fruits per bush than in sectorial pruned plants. The fruit yield 
increased in the first three years of the study, reaching a maximum value in 2021 (7.57 kg per 
bush), whereas an opposite trend was observed in average fruit weight over the years, with the 
lowest value reached in the last harvest year (1.81 g). Fruit weight and soluble solids content 
were not affected by the pruning method, while the content of total acids, total anthocyanins 
and total phenolics were significantly higher in fruit harvested from the SP treatment (0.70%, 
37.04 mg eq mvd-3-G /100 g FW and 1.18 mg eq GA/g FW, respectively) along with the total 
antioxidant capacity (8.39 mg eq AsA /g FW).",
publisher = "Strokovno sadjarsko društvo Slovenije, Jamnikarjeva 101, SI-1000 Ljubljana",
journal = "5. SLOVENSKEGA SADJARSKEGA KONGRESA Z MEDNARODNO UDELEŽBO",
title = "HOW DOES SECTORIAL PRUNING AFFECT THE  VEGETATIVE GROWTH, PRODUCTIVITY AND FRUIT  QUALITY OF SUBSTRATE-GROWN HIGHBUSH  BLUEBERRY CV. 'BLUECROP'?",
pages = "9",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3178"
}
Milivojević, J. M., Radivojević, D., Boskov, D., Dragišić Maksimović, J., Milosavljević, D., Spasojević, S., Veberič, R.,& Mikulič Petkovšek, M.. (2024). HOW DOES SECTORIAL PRUNING AFFECT THE  VEGETATIVE GROWTH, PRODUCTIVITY AND FRUIT  QUALITY OF SUBSTRATE-GROWN HIGHBUSH  BLUEBERRY CV. 'BLUECROP'?. in 5. SLOVENSKEGA SADJARSKEGA KONGRESA Z MEDNARODNO UDELEŽBO
Strokovno sadjarsko društvo Slovenije, Jamnikarjeva 101, SI-1000 Ljubljana., 9.
https://hdl.handle.net/21.15107/rcub_rimsi_3178
Milivojević JM, Radivojević D, Boskov D, Dragišić Maksimović J, Milosavljević D, Spasojević S, Veberič R, Mikulič Petkovšek M. HOW DOES SECTORIAL PRUNING AFFECT THE  VEGETATIVE GROWTH, PRODUCTIVITY AND FRUIT  QUALITY OF SUBSTRATE-GROWN HIGHBUSH  BLUEBERRY CV. 'BLUECROP'?. in 5. SLOVENSKEGA SADJARSKEGA KONGRESA Z MEDNARODNO UDELEŽBO. 2024;:9.
https://hdl.handle.net/21.15107/rcub_rimsi_3178 .
Milivojević, Jasminka M, Radivojević, Dragan, Boskov, Djordje, Dragišić Maksimović, Jelena, Milosavljević, Dragica, Spasojević, Slavica, Veberič, Robert, Mikulič Petkovšek, Maja, "HOW DOES SECTORIAL PRUNING AFFECT THE  VEGETATIVE GROWTH, PRODUCTIVITY AND FRUIT  QUALITY OF SUBSTRATE-GROWN HIGHBUSH  BLUEBERRY CV. 'BLUECROP'?" in 5. SLOVENSKEGA SADJARSKEGA KONGRESA Z MEDNARODNO UDELEŽBO (2024):9,
https://hdl.handle.net/21.15107/rcub_rimsi_3178 .

Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions

Jelušić, Aleksandra; Janakiev, Tamara; Unković, Nikola; Ljaljević Grbić, Milica; Degrassi, Giuliano; Dimkić, Ivica

(Serbian Society for Microbiology, 2024)

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Janakiev, Tamara
AU  - Unković, Nikola
AU  - Ljaljević Grbić, Milica
AU  - Degrassi, Giuliano
AU  - Dimkić, Ivica
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3205
AB  - The soybean is recognized worldwide as one of the most important crops due to its protein- and
oil-rich seeds. Several beneficial bacterial strains, including those of the genus Pseudomonas, are
known to increase plant yield and disease tolerance. The aim of this study was to test the potential of 15 strains of Pseudomonas spp. to promote soybean growth under field conditions. Strains were identified based on 16S rRNA and gyrB sequences, and strains belonging to risk group 1 were further analyzed. Selected strains were tested for plant growth-promoting (PGP) properties, biofilm formation and in vitro antimicrobial activity against various fungal pathogens. PGP activity indicators of the selected strains were evaluated on soybean plants grown in fertilizer-free soil and in soil treated with 70% and 100% fertilizers. Three Pseudomonas spp. strains, P. putida VB131A, P. fulva VB134B and P. rhodesiae VB143S, were selected as PGP candidates. All three strains showed the ability to produce IAA and solubilize phosphorus, while only P. rhodesiae VB143S showed strong lipase and protease activity. Only P. fulva VB134B showed the ability to
produce biofilm and hydrogen cyanide. All three strains showed in vitro antifungal activity against
Alternaria infectoria. P. fulva VB134B and P. rhodesiae VB143S showed antifungal activity against
Alternaria alternata and P. putida VB131A and P. rhodesiae VB143S against Epicoccum nigrum. Only P. fulva VB134B showed activity against Monilinia laxa. Statistically significant differences in
plant height were not observed regardless of the strain or soil treatment used. In terms of first
pod height, the P. fulva VB134B treatment was most effective when applied to soybeans grown
in 100% fertilized soil. In contrast, plants treated with P. rhodesiae VB143S and grown in 100% fertilized soil had the highest number of branches and pods per plant.
PB  - Serbian Society for Microbiology
C3  - XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"
T1  - Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions
EP  - 70
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3205
ER  - 
@conference{
author = "Jelušić, Aleksandra and Janakiev, Tamara and Unković, Nikola and Ljaljević Grbić, Milica and Degrassi, Giuliano and Dimkić, Ivica",
year = "2024",
abstract = "The soybean is recognized worldwide as one of the most important crops due to its protein- and
oil-rich seeds. Several beneficial bacterial strains, including those of the genus Pseudomonas, are
known to increase plant yield and disease tolerance. The aim of this study was to test the potential of 15 strains of Pseudomonas spp. to promote soybean growth under field conditions. Strains were identified based on 16S rRNA and gyrB sequences, and strains belonging to risk group 1 were further analyzed. Selected strains were tested for plant growth-promoting (PGP) properties, biofilm formation and in vitro antimicrobial activity against various fungal pathogens. PGP activity indicators of the selected strains were evaluated on soybean plants grown in fertilizer-free soil and in soil treated with 70% and 100% fertilizers. Three Pseudomonas spp. strains, P. putida VB131A, P. fulva VB134B and P. rhodesiae VB143S, were selected as PGP candidates. All three strains showed the ability to produce IAA and solubilize phosphorus, while only P. rhodesiae VB143S showed strong lipase and protease activity. Only P. fulva VB134B showed the ability to
produce biofilm and hydrogen cyanide. All three strains showed in vitro antifungal activity against
Alternaria infectoria. P. fulva VB134B and P. rhodesiae VB143S showed antifungal activity against
Alternaria alternata and P. putida VB131A and P. rhodesiae VB143S against Epicoccum nigrum. Only P. fulva VB134B showed activity against Monilinia laxa. Statistically significant differences in
plant height were not observed regardless of the strain or soil treatment used. In terms of first
pod height, the P. fulva VB134B treatment was most effective when applied to soybeans grown
in 100% fertilized soil. In contrast, plants treated with P. rhodesiae VB143S and grown in 100% fertilized soil had the highest number of branches and pods per plant.",
publisher = "Serbian Society for Microbiology",
journal = "XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"",
title = "Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3205"
}
Jelušić, A., Janakiev, T., Unković, N., Ljaljević Grbić, M., Degrassi, G.,& Dimkić, I.. (2024). Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions. in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"
Serbian Society for Microbiology., 70-70.
https://hdl.handle.net/21.15107/rcub_rimsi_3205
Jelušić A, Janakiev T, Unković N, Ljaljević Grbić M, Degrassi G, Dimkić I. Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions. in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health". 2024;:70-70.
https://hdl.handle.net/21.15107/rcub_rimsi_3205 .
Jelušić, Aleksandra, Janakiev, Tamara, Unković, Nikola, Ljaljević Grbić, Milica, Degrassi, Giuliano, Dimkić, Ivica, "Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions" in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health" (2024):70-70,
https://hdl.handle.net/21.15107/rcub_rimsi_3205 .

In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia

Iličić, Renata; Jelušić, Aleksandra; Barać, Goran; Nikolić, Dušan; Stošić, Nemanja; Scortichini, Marco; Popović Milovanović, Tatjana

(MDPI, 2024)

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Barać, Goran
AU  - Nikolić, Dušan
AU  - Stošić, Nemanja
AU  - Scortichini, Marco
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3206
AB  - In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1 medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA) using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation, respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall has previously been documented solely in Japan, there is presently no data on its wider occurrence. Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in Serbia will contribute to a better understanding of it at the global level.
PB  - MDPI
T2  - Agronomy
T1  - In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia
IS  - 4
SP  - 851
VL  - 14
DO  - 10.3390/agronomy14040851
ER  - 
@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Barać, Goran and Nikolić, Dušan and Stošić, Nemanja and Scortichini, Marco and Popović Milovanović, Tatjana",
year = "2024",
abstract = "In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1 medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA) using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation, respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall has previously been documented solely in Japan, there is presently no data on its wider occurrence. Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in Serbia will contribute to a better understanding of it at the global level.",
publisher = "MDPI",
journal = "Agronomy",
title = "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia",
number = "4",
pages = "851",
volume = "14",
doi = "10.3390/agronomy14040851"
}
Iličić, R., Jelušić, A., Barać, G., Nikolić, D., Stošić, N., Scortichini, M.,& Popović Milovanović, T.. (2024). In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy
MDPI., 14(4), 851.
https://doi.org/10.3390/agronomy14040851
Iličić R, Jelušić A, Barać G, Nikolić D, Stošić N, Scortichini M, Popović Milovanović T. In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy. 2024;14(4):851.
doi:10.3390/agronomy14040851 .
Iličić, Renata, Jelušić, Aleksandra, Barać, Goran, Nikolić, Dušan, Stošić, Nemanja, Scortichini, Marco, Popović Milovanović, Tatjana, "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia" in Agronomy, 14, no. 4 (2024):851,
https://doi.org/10.3390/agronomy14040851 . .
1

Anatomical Characteristics and Assessment of Wood Fibers Quality of Mature Pedunculate Oak (Quercus robur L.) Trees Grown in Different Environmental Conditions

Lozjanin, Radoslav; Jokanović, Dušan; Nikolić Jokanović, Vesna; Živanović, Kristina; Desimirović, Ivan; Marinković, Marko

(The South-east European forestry (SEEFOR), 2024)

TY  - JOUR
AU  - Lozjanin, Radoslav
AU  - Jokanović, Dušan
AU  - Nikolić Jokanović, Vesna
AU  - Živanović, Kristina
AU  - Desimirović, Ivan
AU  - Marinković, Marko
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3196
AB  - From productive and ecological point of view, Quercus robur L. is an extremely important species and the investigation of its anatomical properties is very significant in terms of its technical and technological utilization. The paper deals with the analysis of the wood fiber characteristics (fiber length, fiber lumen width, double-cell wall thickness, Runkel ratio) of mature pedunculate oak trees in the non-flooded (Management Unit "Vinična-Žeravinac-Puk") and flooded area (Management Unit "GVO 83 b"). One of the aims of the study is to determine the wood fiber quality and the possibility of use in paper and pulp production. When the mean values of the analysed anatomical characteristics are compared, the results show slightly higher values in the non-flooded area, except for the Runkel ratio. The radial pattern of the investigated characteristics depending on the age shows growth in the non-flooded area, while the same tendency in the flooded area was found for the fiber length only. It was recorded that fiber lumen width, double-cell wall thickness and Runkel ratio decreased in the final annual rings. The wood fiber quality for use in pulp and paper production is the greatest in the juvenile zone, unlike sapwood zone, regardless of the site conditions.
PB  - The South-east European forestry (SEEFOR)
T2  - Časopis "Seefor"
T1  - Anatomical Characteristics and Assessment of Wood Fibers Quality of Mature Pedunculate Oak (Quercus robur L.) Trees Grown in Different Environmental Conditions
EP  - 8
IS  - 1
SP  - 1
VL  - 15
DO  - 10.15177/seefor.24-05
ER  - 
@article{
author = "Lozjanin, Radoslav and Jokanović, Dušan and Nikolić Jokanović, Vesna and Živanović, Kristina and Desimirović, Ivan and Marinković, Marko",
year = "2024",
abstract = "From productive and ecological point of view, Quercus robur L. is an extremely important species and the investigation of its anatomical properties is very significant in terms of its technical and technological utilization. The paper deals with the analysis of the wood fiber characteristics (fiber length, fiber lumen width, double-cell wall thickness, Runkel ratio) of mature pedunculate oak trees in the non-flooded (Management Unit "Vinična-Žeravinac-Puk") and flooded area (Management Unit "GVO 83 b"). One of the aims of the study is to determine the wood fiber quality and the possibility of use in paper and pulp production. When the mean values of the analysed anatomical characteristics are compared, the results show slightly higher values in the non-flooded area, except for the Runkel ratio. The radial pattern of the investigated characteristics depending on the age shows growth in the non-flooded area, while the same tendency in the flooded area was found for the fiber length only. It was recorded that fiber lumen width, double-cell wall thickness and Runkel ratio decreased in the final annual rings. The wood fiber quality for use in pulp and paper production is the greatest in the juvenile zone, unlike sapwood zone, regardless of the site conditions.",
publisher = "The South-east European forestry (SEEFOR)",
journal = "Časopis "Seefor"",
title = "Anatomical Characteristics and Assessment of Wood Fibers Quality of Mature Pedunculate Oak (Quercus robur L.) Trees Grown in Different Environmental Conditions",
pages = "8-1",
number = "1",
volume = "15",
doi = "10.15177/seefor.24-05"
}
Lozjanin, R., Jokanović, D., Nikolić Jokanović, V., Živanović, K., Desimirović, I.,& Marinković, M.. (2024). Anatomical Characteristics and Assessment of Wood Fibers Quality of Mature Pedunculate Oak (Quercus robur L.) Trees Grown in Different Environmental Conditions. in Časopis "Seefor"
The South-east European forestry (SEEFOR)., 15(1), 1-8.
https://doi.org/10.15177/seefor.24-05
Lozjanin R, Jokanović D, Nikolić Jokanović V, Živanović K, Desimirović I, Marinković M. Anatomical Characteristics and Assessment of Wood Fibers Quality of Mature Pedunculate Oak (Quercus robur L.) Trees Grown in Different Environmental Conditions. in Časopis "Seefor". 2024;15(1):1-8.
doi:10.15177/seefor.24-05 .
Lozjanin, Radoslav, Jokanović, Dušan, Nikolić Jokanović, Vesna, Živanović, Kristina, Desimirović, Ivan, Marinković, Marko, "Anatomical Characteristics and Assessment of Wood Fibers Quality of Mature Pedunculate Oak (Quercus robur L.) Trees Grown in Different Environmental Conditions" in Časopis "Seefor", 15, no. 1 (2024):1-8,
https://doi.org/10.15177/seefor.24-05 . .

Silicon nanoparticles in sustainable agriculture: synthesis, absorption, and plant stress alleviation

Yan, Gouchao; Huang, Qingying; Zhao, Shuaijing; Xu, Yunmin; He, Yong; Nikolic, Miroslav; Nikolić, Nina; Liang, Yongchao; Zhu, Zhujun

(Frontiers, 2024)

TY  - JOUR
AU  - Yan, Gouchao
AU  - Huang, Qingying
AU  - Zhao, Shuaijing
AU  - Xu, Yunmin
AU  - He, Yong
AU  - Nikolic, Miroslav
AU  - Nikolić, Nina
AU  - Liang, Yongchao
AU  - Zhu, Zhujun
PY  - 2024
UR  - https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1393458/full
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3192
AB  - Silicon (Si) is a widely recognized beneficial element in plants. With the emergence of nanotechnology in agriculture, silicon nanoparticles (SiNPs) demonstrate promising applicability in sustainable agriculture. Particularly, the application of SiNPs has proven to be a high-efficiency and cost-effective strategy for protecting plant against various biotic and abiotic stresses such as insect pests, pathogen diseases, metal stress, drought stress, and salt stress. To date, rapid progress has been made in unveiling the multiple functions and related mechanisms of SiNPs in promoting the sustainability of agricultural production in the recent decade, while a comprehensive summary is still lacking. Here, the review provides an up-to-date overview of the synthesis, uptake and translocation, and application of SiNPs in alleviating stresses aiming for the reasonable usage of SiNPs in nano-enabled agriculture. The major points are listed as following: (1) SiNPs can be synthesized by using physical, chemical, and biological (green synthesis) approaches, while green synthesis using agricultural wastes as raw materials is more suitable for large-scale production and recycling agriculture. (2) The uptake and translocation of SiNPs in plants differs significantly from that of Si, which is determined by plant factors and the properties of SiNPs. (3) Under stressful conditions, SiNPs can regulate plant stress acclimation at morphological, physiological, and molecular levels as growth stimulator; as well as deliver pesticides and plant growth regulating chemicals as nanocarrier, thereby enhancing plant growth and yield. (4) Several key issues deserve further investigation including effective approaches of SiNPs synthesis and modification, molecular basis of SiNPs-induced plant stress resistance, and systematic effects of SiNPs on agricultural ecosystem.
PB  - Frontiers
T2  - Frontiers in Plant Science
T1  - Silicon nanoparticles in sustainable agriculture: synthesis, absorption, and plant stress alleviation
SP  - 1393458
VL  - 15
DO  - https://doi.org/10.3389/fpls.2024.1393458
ER  - 
@article{
author = "Yan, Gouchao and Huang, Qingying and Zhao, Shuaijing and Xu, Yunmin and He, Yong and Nikolic, Miroslav and Nikolić, Nina and Liang, Yongchao and Zhu, Zhujun",
year = "2024",
abstract = "Silicon (Si) is a widely recognized beneficial element in plants. With the emergence of nanotechnology in agriculture, silicon nanoparticles (SiNPs) demonstrate promising applicability in sustainable agriculture. Particularly, the application of SiNPs has proven to be a high-efficiency and cost-effective strategy for protecting plant against various biotic and abiotic stresses such as insect pests, pathogen diseases, metal stress, drought stress, and salt stress. To date, rapid progress has been made in unveiling the multiple functions and related mechanisms of SiNPs in promoting the sustainability of agricultural production in the recent decade, while a comprehensive summary is still lacking. Here, the review provides an up-to-date overview of the synthesis, uptake and translocation, and application of SiNPs in alleviating stresses aiming for the reasonable usage of SiNPs in nano-enabled agriculture. The major points are listed as following: (1) SiNPs can be synthesized by using physical, chemical, and biological (green synthesis) approaches, while green synthesis using agricultural wastes as raw materials is more suitable for large-scale production and recycling agriculture. (2) The uptake and translocation of SiNPs in plants differs significantly from that of Si, which is determined by plant factors and the properties of SiNPs. (3) Under stressful conditions, SiNPs can regulate plant stress acclimation at morphological, physiological, and molecular levels as growth stimulator; as well as deliver pesticides and plant growth regulating chemicals as nanocarrier, thereby enhancing plant growth and yield. (4) Several key issues deserve further investigation including effective approaches of SiNPs synthesis and modification, molecular basis of SiNPs-induced plant stress resistance, and systematic effects of SiNPs on agricultural ecosystem.",
publisher = "Frontiers",
journal = "Frontiers in Plant Science",
title = "Silicon nanoparticles in sustainable agriculture: synthesis, absorption, and plant stress alleviation",
pages = "1393458",
volume = "15",
doi = "https://doi.org/10.3389/fpls.2024.1393458"
}
Yan, G., Huang, Q., Zhao, S., Xu, Y., He, Y., Nikolic, M., Nikolić, N., Liang, Y.,& Zhu, Z.. (2024). Silicon nanoparticles in sustainable agriculture: synthesis, absorption, and plant stress alleviation. in Frontiers in Plant Science
Frontiers., 15, 1393458.
https://doi.org/https://doi.org/10.3389/fpls.2024.1393458
Yan G, Huang Q, Zhao S, Xu Y, He Y, Nikolic M, Nikolić N, Liang Y, Zhu Z. Silicon nanoparticles in sustainable agriculture: synthesis, absorption, and plant stress alleviation. in Frontiers in Plant Science. 2024;15:1393458.
doi:https://doi.org/10.3389/fpls.2024.1393458 .
Yan, Gouchao, Huang, Qingying, Zhao, Shuaijing, Xu, Yunmin, He, Yong, Nikolic, Miroslav, Nikolić, Nina, Liang, Yongchao, Zhu, Zhujun, "Silicon nanoparticles in sustainable agriculture: synthesis, absorption, and plant stress alleviation" in Frontiers in Plant Science, 15 (2024):1393458,
https://doi.org/https://doi.org/10.3389/fpls.2024.1393458 . .

Svojstva visokotemperaturnih protonskih provodnika na bazi barijum-cerijum-indijum-oksida

Malešević, Aleksandar

(2024)

TY  - THES
AU  - Malešević, Aleksandar
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3189
AB  - U okviru ove doktorske disertacije je ispitan uticaj koncentracije dopanta na svojstva BaCe1−xInxO3–δ (BCI, x = 0,05; 0,10; 0,15; 0,20; 0,25; 0,30; 0,35 i 0,40) kao protonskog provodnika. Struktura i fazni sastav sintetisanih prahova dobijenih metodom samosagorevanja su utvrđeni rendgenskom difrakcionom analizom, pri čemu je pokazano da prahovi sa ≥ 25 % (mol.) indijuma sadrže In2O3 kao sekundarnu fazu. Optimizacijom postupka sinterovanja je utvrđeno da je termički tretman na 1300 °C tokom 5 h optimalan za dobijanje guste neporozne keramike (98 % teorijske vrednosti). Mikrostruktura je analizirana skanirajućom elektronskom mikroskopijom (SEM). Povećanje temperature sinterovanja dovodi do nastanka većih zrna, dok povećanje koncentracije indijuma do 25 % (mol.) dovodi do porasta zrna, a iznad te koncentracije nema daljeg uticaja na veličinu zrna. Izlaganjem sinterovanog uzorka atmosferi CO2 tokom 5 h na 700 °C je pokazano da indijum u potpunosti suzbija degradaciju BaCeO3 već u koncentraciji od 15 % (mol.). Električnim merenjima u atmosferi vlažnog vodonika je utvrđeno da maksimalna ukupna provodljivost iznosi 5 × 10−3 S cm–1 na 700 °C za uzorak BaCe0,75In0,25O3–δ (BCI25), zbog čega je ovaj materijal odabran kao najbolji kandidat za elektrolit gorivne ćelije na bazi oksida u čvrstom stanju. Dobijena gorivna ćelija Ni-BaCe0,75In0,25O3−δ║BaCe0,75In0,25O3−δ║LSCF-BaCe0,75In0,25O3−δ (LSCF = lantan-stroncijum-kobalt-gvožđe-oksid, La0,8Sr0,2Co0,8Fe0,2O3) je pokazala maksimalnu gustinu izlazne snage od 264 mW cm–2 na 700 °C, koristeći vlažan vodonik kao gorivo. Ovakav rezultat ukazuje na stabilnost i funkcionalnost BCI25 kao elektrolita za gorivne ćelije.
Ispitivanjem električnih svojstava BCI25 u atmosferama različitog sastava je uočeno da dolazi do brze promene impedanse prilikom promene parcijalnog pritiska vodene pare, što čini ovaj materijal pogodnim za primenu kao visokotemperaturnog senzora vodene pare. Senzorska svojstva BCI25 u vidu poroznog filma i sinterovane keramike su ispitana u temperaturnom opsegu 250 °C – 700 °C. Film debljine 30 μm, napravljen od praha kalcinisanog na 1050 °C, je pokazao osetljivost uporedivu sa osetljivošću sinterovanog uzorka, ali sa značajno kraćim vremenom odziva i oporavka. Pokazalo se da je najbolja osetljivost senzora na temperaturi od 500 °C. Osetljivost filma se postepeno smanjivala sa smanjenjem parcijalnog pritiska vodene pare pH2O, ali je bila značajna i pri parcijalnom pritisku vodene pare od 200 Pa. Pad provodljivosti je zavisio logaritamski od parcijalnog pritiska vodene pare sa nagibom od 0,52 što je blisko tipičnoj vrednosti za protonski tip provođenja u tom temperaturnom opsegu. Posle nekoliko ciklusa, test ponovne upotrebe je pokazao skoro nepromenjen odnos između impedanse u suvoj i vlažnoj atmosferi (pH2O = 2,34 kPa), što je ukazalo na dobru stabilnost i osetljivost BCI25 kao potencijalnog visokotemperaturnog senzora vodene pare.
T2  - Univerzitet u Beogradu - Hemijski fakultet
T1  - Svojstva visokotemperaturnih protonskih provodnika na bazi barijum-cerijum-indijum-oksida
T1  - Properties of high-temperature proton conductors based on barium cerium indium oxide
EP  - 105
SP  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3189
ER  - 
@phdthesis{
author = "Malešević, Aleksandar",
year = "2024",
abstract = "U okviru ove doktorske disertacije je ispitan uticaj koncentracije dopanta na svojstva BaCe1−xInxO3–δ (BCI, x = 0,05; 0,10; 0,15; 0,20; 0,25; 0,30; 0,35 i 0,40) kao protonskog provodnika. Struktura i fazni sastav sintetisanih prahova dobijenih metodom samosagorevanja su utvrđeni rendgenskom difrakcionom analizom, pri čemu je pokazano da prahovi sa ≥ 25 % (mol.) indijuma sadrže In2O3 kao sekundarnu fazu. Optimizacijom postupka sinterovanja je utvrđeno da je termički tretman na 1300 °C tokom 5 h optimalan za dobijanje guste neporozne keramike (98 % teorijske vrednosti). Mikrostruktura je analizirana skanirajućom elektronskom mikroskopijom (SEM). Povećanje temperature sinterovanja dovodi do nastanka većih zrna, dok povećanje koncentracije indijuma do 25 % (mol.) dovodi do porasta zrna, a iznad te koncentracije nema daljeg uticaja na veličinu zrna. Izlaganjem sinterovanog uzorka atmosferi CO2 tokom 5 h na 700 °C je pokazano da indijum u potpunosti suzbija degradaciju BaCeO3 već u koncentraciji od 15 % (mol.). Električnim merenjima u atmosferi vlažnog vodonika je utvrđeno da maksimalna ukupna provodljivost iznosi 5 × 10−3 S cm–1 na 700 °C za uzorak BaCe0,75In0,25O3–δ (BCI25), zbog čega je ovaj materijal odabran kao najbolji kandidat za elektrolit gorivne ćelije na bazi oksida u čvrstom stanju. Dobijena gorivna ćelija Ni-BaCe0,75In0,25O3−δ║BaCe0,75In0,25O3−δ║LSCF-BaCe0,75In0,25O3−δ (LSCF = lantan-stroncijum-kobalt-gvožđe-oksid, La0,8Sr0,2Co0,8Fe0,2O3) je pokazala maksimalnu gustinu izlazne snage od 264 mW cm–2 na 700 °C, koristeći vlažan vodonik kao gorivo. Ovakav rezultat ukazuje na stabilnost i funkcionalnost BCI25 kao elektrolita za gorivne ćelije.
Ispitivanjem električnih svojstava BCI25 u atmosferama različitog sastava je uočeno da dolazi do brze promene impedanse prilikom promene parcijalnog pritiska vodene pare, što čini ovaj materijal pogodnim za primenu kao visokotemperaturnog senzora vodene pare. Senzorska svojstva BCI25 u vidu poroznog filma i sinterovane keramike su ispitana u temperaturnom opsegu 250 °C – 700 °C. Film debljine 30 μm, napravljen od praha kalcinisanog na 1050 °C, je pokazao osetljivost uporedivu sa osetljivošću sinterovanog uzorka, ali sa značajno kraćim vremenom odziva i oporavka. Pokazalo se da je najbolja osetljivost senzora na temperaturi od 500 °C. Osetljivost filma se postepeno smanjivala sa smanjenjem parcijalnog pritiska vodene pare pH2O, ali je bila značajna i pri parcijalnom pritisku vodene pare od 200 Pa. Pad provodljivosti je zavisio logaritamski od parcijalnog pritiska vodene pare sa nagibom od 0,52 što je blisko tipičnoj vrednosti za protonski tip provođenja u tom temperaturnom opsegu. Posle nekoliko ciklusa, test ponovne upotrebe je pokazao skoro nepromenjen odnos između impedanse u suvoj i vlažnoj atmosferi (pH2O = 2,34 kPa), što je ukazalo na dobru stabilnost i osetljivost BCI25 kao potencijalnog visokotemperaturnog senzora vodene pare.",
journal = "Univerzitet u Beogradu - Hemijski fakultet",
title = "Svojstva visokotemperaturnih protonskih provodnika na bazi barijum-cerijum-indijum-oksida, Properties of high-temperature proton conductors based on barium cerium indium oxide",
pages = "105-1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3189"
}
Malešević, A.. (2024). Svojstva visokotemperaturnih protonskih provodnika na bazi barijum-cerijum-indijum-oksida. in Univerzitet u Beogradu - Hemijski fakultet, 1-105.
https://hdl.handle.net/21.15107/rcub_rimsi_3189
Malešević A. Svojstva visokotemperaturnih protonskih provodnika na bazi barijum-cerijum-indijum-oksida. in Univerzitet u Beogradu - Hemijski fakultet. 2024;:1-105.
https://hdl.handle.net/21.15107/rcub_rimsi_3189 .
Malešević, Aleksandar, "Svojstva visokotemperaturnih protonskih provodnika na bazi barijum-cerijum-indijum-oksida" in Univerzitet u Beogradu - Hemijski fakultet (2024):1-105,
https://hdl.handle.net/21.15107/rcub_rimsi_3189 .

Phytoremediation Potential of Different Genotypes of Salix alba and S. viminalis

Urošević, Jelena; Stanković, Dragica; Jokanović, Dušan; Trivan, Goran; Rodzkin, Aleh; Jović, Đorđe; Jovanović, Filip

(MDPI, 2024)

TY  - JOUR
AU  - Urošević, Jelena
AU  - Stanković, Dragica
AU  - Jokanović, Dušan
AU  - Trivan, Goran
AU  - Rodzkin, Aleh
AU  - Jović, Đorđe
AU  - Jovanović, Filip
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3183
AB  - Elevated concentrations of heavy metals result in soil degradation, a reduction in plant
yields, and a lower quality of agricultural products, which directly endangers people, animals, and
the ecosystem. The potential of three clones of Salix alba (347, NS 73/6, and B-44) and one genotype of
S. viminalis for the phytoextraction of heavy metals was investigated, with the aim of identifying the
most physiologically suitable willow genotypes for use in soil phytoremediation. The experiment was
placed on the contaminated soil substrate collected in Kolubara Mining Basin (Serbia), enriched by
high loads of heavy metal salts, and a control medium. Significant differences in the concentrations
of heavy metals were recorded between the contaminated and control plant material, especially
when it comes to nickel (Ni), copper (Cu), cadmium (Cd), and lead (Pb), confirming that S. alba and
S. viminalis are hyperaccumulator species of heavy metals. Clone 347 shows the greatest uptake of
Cd and chromium (Cr), and clone B-44 takes up these metals only to a lesser extent, while clone
NS 73/6 shows a less pronounced uptake of Cr. The roots have the greatest ability to accumulate
Ni and Pb, Cu is absorbed by all plant organs, while Cd is absorbed by the leaves. The organ that
showed the greatest ability to accumulate heavy metals was the root, which means that willows have
a limited power to translocate heavy metals to above-ground organs. The studied genotypes of S. alba
have a higher potential for the phytostabilization of Cu and Cd, as well as the phytoextraction of
Cd, compared with S. viminalis. The results confirm the assumption of differences between different
willow genotypes in terms of the ability to phytoextract certain heavy metals from soil, which is
important information when selecting genotypes for soil phytoremediation.
PB  - MDPI
T2  - Plants
T1  - Phytoremediation Potential of Different Genotypes of Salix alba and S. viminalis
IS  - 5
SP  - 735
VL  - 13
DO  - 10.3390/plants13050735
ER  - 
@article{
author = "Urošević, Jelena and Stanković, Dragica and Jokanović, Dušan and Trivan, Goran and Rodzkin, Aleh and Jović, Đorđe and Jovanović, Filip",
year = "2024",
abstract = "Elevated concentrations of heavy metals result in soil degradation, a reduction in plant
yields, and a lower quality of agricultural products, which directly endangers people, animals, and
the ecosystem. The potential of three clones of Salix alba (347, NS 73/6, and B-44) and one genotype of
S. viminalis for the phytoextraction of heavy metals was investigated, with the aim of identifying the
most physiologically suitable willow genotypes for use in soil phytoremediation. The experiment was
placed on the contaminated soil substrate collected in Kolubara Mining Basin (Serbia), enriched by
high loads of heavy metal salts, and a control medium. Significant differences in the concentrations
of heavy metals were recorded between the contaminated and control plant material, especially
when it comes to nickel (Ni), copper (Cu), cadmium (Cd), and lead (Pb), confirming that S. alba and
S. viminalis are hyperaccumulator species of heavy metals. Clone 347 shows the greatest uptake of
Cd and chromium (Cr), and clone B-44 takes up these metals only to a lesser extent, while clone
NS 73/6 shows a less pronounced uptake of Cr. The roots have the greatest ability to accumulate
Ni and Pb, Cu is absorbed by all plant organs, while Cd is absorbed by the leaves. The organ that
showed the greatest ability to accumulate heavy metals was the root, which means that willows have
a limited power to translocate heavy metals to above-ground organs. The studied genotypes of S. alba
have a higher potential for the phytostabilization of Cu and Cd, as well as the phytoextraction of
Cd, compared with S. viminalis. The results confirm the assumption of differences between different
willow genotypes in terms of the ability to phytoextract certain heavy metals from soil, which is
important information when selecting genotypes for soil phytoremediation.",
publisher = "MDPI",
journal = "Plants",
title = "Phytoremediation Potential of Different Genotypes of Salix alba and S. viminalis",
number = "5",
pages = "735",
volume = "13",
doi = "10.3390/plants13050735"
}
Urošević, J., Stanković, D., Jokanović, D., Trivan, G., Rodzkin, A., Jović, Đ.,& Jovanović, F.. (2024). Phytoremediation Potential of Different Genotypes of Salix alba and S. viminalis. in Plants
MDPI., 13(5), 735.
https://doi.org/10.3390/plants13050735
Urošević J, Stanković D, Jokanović D, Trivan G, Rodzkin A, Jović Đ, Jovanović F. Phytoremediation Potential of Different Genotypes of Salix alba and S. viminalis. in Plants. 2024;13(5):735.
doi:10.3390/plants13050735 .
Urošević, Jelena, Stanković, Dragica, Jokanović, Dušan, Trivan, Goran, Rodzkin, Aleh, Jović, Đorđe, Jovanović, Filip, "Phytoremediation Potential of Different Genotypes of Salix alba and S. viminalis" in Plants, 13, no. 5 (2024):735,
https://doi.org/10.3390/plants13050735 . .

Supercapacitor electrodes: Is nickel foam the right substrate for active materials?

Dojčinović, Milena; Stojković Simatović, Ivana; Nikolić, Maria Vesna

(MDPI, 2024)

TY  - JOUR
AU  - Dojčinović, Milena
AU  - Stojković Simatović, Ivana
AU  - Nikolić, Maria Vesna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3185
AB  - Ni foam is an extensively used current collector and substrate in investigations of electrochemically active materials such as supercapacitors and electrocatalysts for oxygen and hydrogen evolution reactions. This material is relatively cheap, porous, and conductive and has a large specific surface area, all of which make it a good substrate. We investigated Ni-Mg ferrites and NiMn2O4 as active materials for electrochemical energy storage. These materials, when loaded on Ni foam, gave promising capacitance values: 172 F/g (at 2 mV/s) for NiMn2O4 in 6 M KOH and 242 F/g (at 2 mV/s) for MgFe2O4 in 3 M KOH. Nevertheless, during the authors’ work, many experimental problems occurred. Inconsistencies in the results directed further investigation towards measuring the capacitance of the active materials using GCE and platinum electrodes as substrates to discover if Ni foam was the culprit of the inconsistencies. When non-nickel substrates were used, both NiMn2O4 and MgFe2O4 showed reduced capacitance. Experimental problems associated with the utilization of Ni foam as a substrate for active materials in supercapacitor electrodes are discussed here, combined with other problems already addressed in the scientific literature
PB  - MDPI
T2  - Materials
T1  - Supercapacitor electrodes: Is nickel foam the right substrate for active materials?
IS  - 6
SP  - 1292
VL  - 17
DO  - 10.3390/ma17061292
ER  - 
@article{
author = "Dojčinović, Milena and Stojković Simatović, Ivana and Nikolić, Maria Vesna",
year = "2024",
abstract = "Ni foam is an extensively used current collector and substrate in investigations of electrochemically active materials such as supercapacitors and electrocatalysts for oxygen and hydrogen evolution reactions. This material is relatively cheap, porous, and conductive and has a large specific surface area, all of which make it a good substrate. We investigated Ni-Mg ferrites and NiMn2O4 as active materials for electrochemical energy storage. These materials, when loaded on Ni foam, gave promising capacitance values: 172 F/g (at 2 mV/s) for NiMn2O4 in 6 M KOH and 242 F/g (at 2 mV/s) for MgFe2O4 in 3 M KOH. Nevertheless, during the authors’ work, many experimental problems occurred. Inconsistencies in the results directed further investigation towards measuring the capacitance of the active materials using GCE and platinum electrodes as substrates to discover if Ni foam was the culprit of the inconsistencies. When non-nickel substrates were used, both NiMn2O4 and MgFe2O4 showed reduced capacitance. Experimental problems associated with the utilization of Ni foam as a substrate for active materials in supercapacitor electrodes are discussed here, combined with other problems already addressed in the scientific literature",
publisher = "MDPI",
journal = "Materials",
title = "Supercapacitor electrodes: Is nickel foam the right substrate for active materials?",
number = "6",
pages = "1292",
volume = "17",
doi = "10.3390/ma17061292"
}
Dojčinović, M., Stojković Simatović, I.,& Nikolić, M. V.. (2024). Supercapacitor electrodes: Is nickel foam the right substrate for active materials?. in Materials
MDPI., 17(6), 1292.
https://doi.org/10.3390/ma17061292
Dojčinović M, Stojković Simatović I, Nikolić MV. Supercapacitor electrodes: Is nickel foam the right substrate for active materials?. in Materials. 2024;17(6):1292.
doi:10.3390/ma17061292 .
Dojčinović, Milena, Stojković Simatović, Ivana, Nikolić, Maria Vesna, "Supercapacitor electrodes: Is nickel foam the right substrate for active materials?" in Materials, 17, no. 6 (2024):1292,
https://doi.org/10.3390/ma17061292 . .

VARIABILITY OF ANATOMICAL AND MORPHOLOGICAL TRAITS OF PINUS NIGRA AND PINUS SYLVESTRIS SEEDLINGS AFFECTED BY DIFFERENT CONTAINER TYPE

Jokanović, Dušan; Devetaković, Jovana; Nikolić Jokanović, Vesna; Živanović, Kristina; Mijatović, Ljubica; Desimirović, Ivan

(Wood Research – VUPC, a.s. (Pulp and Paper Research Institute), 2024)

TY  - JOUR
AU  - Jokanović, Dušan
AU  - Devetaković, Jovana
AU  - Nikolić Jokanović, Vesna
AU  - Živanović, Kristina
AU  - Mijatović, Ljubica
AU  - Desimirović, Ivan
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3184
AB  - In the paper was analysed the influence of three different container types, used for
cultivation of Pinus nigra and Pinus sylvestris seedlings, on dimensions of their anatomical
(resin ducts width, resin ducts number, tracheid number, tracheid width, wood rays height) and
morphological (height, root collar diameter, sturdiness coefficient) elements, and on proportion
of wood, bark and pith, as well. Two-factorial ANOVA showed that container type affects a lot
all investigated anatomical traits by both species, but on the other side, these species varied
between each other just in terms of tracheid width and wood rays height. Based on descriptive
statistics, significantly lower values of all studied anatomical elements were recorded by
biodegradable compared to plastic containers. As for P. nigra seedlings, they showed the best
anatomical performance in Plantagrah I, while Hiko V-120 SS was the most suitable for
P. sylvestris. The highest proportion of pith and bark was recorded in biodegradable container.
As for morphological parameters, such as height and root collar diameter, higher values were
recorded by plastic containers.
PB  - Wood Research – VUPC, a.s. (Pulp and Paper Research Institute)
T2  - Časopis "Wood Research"
T1  - VARIABILITY OF ANATOMICAL AND MORPHOLOGICAL TRAITS OF PINUS NIGRA AND PINUS SYLVESTRIS SEEDLINGS AFFECTED BY DIFFERENT CONTAINER TYPE
EP  - 49
IS  - 1
SP  - 37
VL  - 69
DO  - 10.37763/wr.1336-4561/69.1.3749
ER  - 
@article{
author = "Jokanović, Dušan and Devetaković, Jovana and Nikolić Jokanović, Vesna and Živanović, Kristina and Mijatović, Ljubica and Desimirović, Ivan",
year = "2024",
abstract = "In the paper was analysed the influence of three different container types, used for
cultivation of Pinus nigra and Pinus sylvestris seedlings, on dimensions of their anatomical
(resin ducts width, resin ducts number, tracheid number, tracheid width, wood rays height) and
morphological (height, root collar diameter, sturdiness coefficient) elements, and on proportion
of wood, bark and pith, as well. Two-factorial ANOVA showed that container type affects a lot
all investigated anatomical traits by both species, but on the other side, these species varied
between each other just in terms of tracheid width and wood rays height. Based on descriptive
statistics, significantly lower values of all studied anatomical elements were recorded by
biodegradable compared to plastic containers. As for P. nigra seedlings, they showed the best
anatomical performance in Plantagrah I, while Hiko V-120 SS was the most suitable for
P. sylvestris. The highest proportion of pith and bark was recorded in biodegradable container.
As for morphological parameters, such as height and root collar diameter, higher values were
recorded by plastic containers.",
publisher = "Wood Research – VUPC, a.s. (Pulp and Paper Research Institute)",
journal = "Časopis "Wood Research"",
title = "VARIABILITY OF ANATOMICAL AND MORPHOLOGICAL TRAITS OF PINUS NIGRA AND PINUS SYLVESTRIS SEEDLINGS AFFECTED BY DIFFERENT CONTAINER TYPE",
pages = "49-37",
number = "1",
volume = "69",
doi = "10.37763/wr.1336-4561/69.1.3749"
}
Jokanović, D., Devetaković, J., Nikolić Jokanović, V., Živanović, K., Mijatović, L.,& Desimirović, I.. (2024). VARIABILITY OF ANATOMICAL AND MORPHOLOGICAL TRAITS OF PINUS NIGRA AND PINUS SYLVESTRIS SEEDLINGS AFFECTED BY DIFFERENT CONTAINER TYPE. in Časopis "Wood Research"
Wood Research – VUPC, a.s. (Pulp and Paper Research Institute)., 69(1), 37-49.
https://doi.org/10.37763/wr.1336-4561/69.1.3749
Jokanović D, Devetaković J, Nikolić Jokanović V, Živanović K, Mijatović L, Desimirović I. VARIABILITY OF ANATOMICAL AND MORPHOLOGICAL TRAITS OF PINUS NIGRA AND PINUS SYLVESTRIS SEEDLINGS AFFECTED BY DIFFERENT CONTAINER TYPE. in Časopis "Wood Research". 2024;69(1):37-49.
doi:10.37763/wr.1336-4561/69.1.3749 .
Jokanović, Dušan, Devetaković, Jovana, Nikolić Jokanović, Vesna, Živanović, Kristina, Mijatović, Ljubica, Desimirović, Ivan, "VARIABILITY OF ANATOMICAL AND MORPHOLOGICAL TRAITS OF PINUS NIGRA AND PINUS SYLVESTRIS SEEDLINGS AFFECTED BY DIFFERENT CONTAINER TYPE" in Časopis "Wood Research", 69, no. 1 (2024):37-49,
https://doi.org/10.37763/wr.1336-4561/69.1.3749 . .

Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass

Žižić, Milan; Atlagić, Kristina; Karaman, Maja; Živić, Miroslav; Stanić, Marina; Maksimović, Vuk; Zakrzewska, Joanna

(Elsevier Gmbh, Munich, 2024)

TY  - JOUR
AU  - Žižić, Milan
AU  - Atlagić, Kristina
AU  - Karaman, Maja
AU  - Živić, Miroslav
AU  - Stanić, Marina
AU  - Maksimović, Vuk
AU  - Zakrzewska, Joanna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3165
AB  - BACKGROUND
Fungi absorb and solubilize a broad spectrum of heavy metals such as vanadium (V), which makes them a main route of its entry into the biosphere. V as vanadate (V5+) is a potential medical agent due to its many metabolic actions such as interaction with phosphates in the cell, and especially its insulin-mimetic activity. Antidiabetic activity of V-enriched fungi has been studied in recent years, but the biological and chemical bases of vanadium action and status in fungi in general are poorly understood, with almost no information on edible fungi. 
METHODS
This manuscript gives a deeper insight into the interaction of V5+ with Coprinellus truncorum, an edible autochthonous species widely distributed in Europe and North America. Vanadium uptake and accumulation as V5+ was studied by 51V NMR, while the reducing abilities of the mycelium were determined by EPR. 31P NMR was used to determine its effects on the metabolism of phosphate compounds, with particular focus on phosphate sugars identified using HPLC.
RESULTS
Vanadate enters the mycelium in monomeric form and shows no immediate detrimental effects on intracellular pH or polyphosphate (PPc) levels, even when applied at physiologically high concentrations (20 mM Na3VO4). Once absorbed, it is partially reduced to less toxic vanadyl (V4+) with notable unreduced portion, which leads to a large increase in phosphorylated sugar levels, especially glucose-1-phosphate (G1P) and fructose-6-phosphate (F6P).
CONCLUSIONS
Preservation of pH and especially PPc reflects maintenance of the energy status of the mycelium, i.e., its tolerance to high V5+ concentrations. Rise in G1P and F6P levels implies that the main targets of V5+ are most likely phosphoglucomutase and phosphoglucokinase(s), enzymes involved in early stages of G6P transformation in glycolysis and glycogen metabolism. This study recommends C. truncorum for further investigation as a potential antidiabetic agent.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Trace Elements in Biology and Medicine
T1  - Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass
EP  - 127386
SP  - 127381
VL  - 83
DO  - 10.1016/j.jtemb.2024.127381
ER  - 
@article{
author = "Žižić, Milan and Atlagić, Kristina and Karaman, Maja and Živić, Miroslav and Stanić, Marina and Maksimović, Vuk and Zakrzewska, Joanna",
year = "2024",
abstract = "BACKGROUND
Fungi absorb and solubilize a broad spectrum of heavy metals such as vanadium (V), which makes them a main route of its entry into the biosphere. V as vanadate (V5+) is a potential medical agent due to its many metabolic actions such as interaction with phosphates in the cell, and especially its insulin-mimetic activity. Antidiabetic activity of V-enriched fungi has been studied in recent years, but the biological and chemical bases of vanadium action and status in fungi in general are poorly understood, with almost no information on edible fungi. 
METHODS
This manuscript gives a deeper insight into the interaction of V5+ with Coprinellus truncorum, an edible autochthonous species widely distributed in Europe and North America. Vanadium uptake and accumulation as V5+ was studied by 51V NMR, while the reducing abilities of the mycelium were determined by EPR. 31P NMR was used to determine its effects on the metabolism of phosphate compounds, with particular focus on phosphate sugars identified using HPLC.
RESULTS
Vanadate enters the mycelium in monomeric form and shows no immediate detrimental effects on intracellular pH or polyphosphate (PPc) levels, even when applied at physiologically high concentrations (20 mM Na3VO4). Once absorbed, it is partially reduced to less toxic vanadyl (V4+) with notable unreduced portion, which leads to a large increase in phosphorylated sugar levels, especially glucose-1-phosphate (G1P) and fructose-6-phosphate (F6P).
CONCLUSIONS
Preservation of pH and especially PPc reflects maintenance of the energy status of the mycelium, i.e., its tolerance to high V5+ concentrations. Rise in G1P and F6P levels implies that the main targets of V5+ are most likely phosphoglucomutase and phosphoglucokinase(s), enzymes involved in early stages of G6P transformation in glycolysis and glycogen metabolism. This study recommends C. truncorum for further investigation as a potential antidiabetic agent.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Trace Elements in Biology and Medicine",
title = "Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass",
pages = "127386-127381",
volume = "83",
doi = "10.1016/j.jtemb.2024.127381"
}
Žižić, M., Atlagić, K., Karaman, M., Živić, M., Stanić, M., Maksimović, V.,& Zakrzewska, J.. (2024). Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass. in Journal of Trace Elements in Biology and Medicine
Elsevier Gmbh, Munich., 83, 127381-127386.
https://doi.org/10.1016/j.jtemb.2024.127381
Žižić M, Atlagić K, Karaman M, Živić M, Stanić M, Maksimović V, Zakrzewska J. Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass. in Journal of Trace Elements in Biology and Medicine. 2024;83:127381-127386.
doi:10.1016/j.jtemb.2024.127381 .
Žižić, Milan, Atlagić, Kristina, Karaman, Maja, Živić, Miroslav, Stanić, Marina, Maksimović, Vuk, Zakrzewska, Joanna, "Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass" in Journal of Trace Elements in Biology and Medicine, 83 (2024):127381-127386,
https://doi.org/10.1016/j.jtemb.2024.127381 . .
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