Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200358 (BioSense Institute)

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Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200358 (BioSense Institute) (en)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije, Ugovor br. 200358 (BioSense institut) (sr_RS)
Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 200358 (BioSense институт) (sr)
Authors

Publications

Electrical and sensing properties of indium-doped barium cerate

Malešević, Aleksandar; Radojković, Aleksandar; Žunić, Milan; Savic, Slavica; Perać, Sanja; Branković, Zorica; Branković, Goran

(Elsevier Ltd, 2023)

TY  - JOUR
AU  - Malešević, Aleksandar
AU  - Radojković, Aleksandar
AU  - Žunić, Milan
AU  - Savic, Slavica
AU  - Perać, Sanja
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2299
AB  - Systematic analysis of electrical characteristics of BaCe0.75In0.25O3-δ (BCI25) sintered sample was performed in a
dry and a wet argon atmosphere in the 250 ◦C–700 ◦C temperature range. The water vapor sensing properties of
BCI25 porous film and its response and recovery times were investigated under different conditions of temperature
and water vapor concentration. The 30 μm thick film obtained from the powder calcined at 1050 ◦C
exhibited sensitivity comparable to that of the sintered sample with a significantly shorter response and recovery
times. While the sensitivity of the film gradually decreased with a decrease in partial pressure of water vapor (p
(H2O)), a noticeable sensitivity was still observed at p(H2O) of 200 Pa. Decrease in conductivity depended
logarithmically on the partial pressure of water with the slope of 0.52 that is close to the theoretical value. After
several cycles, the reusability test proved an almost unchanged ratio between the impedance value in the dry and
the wet Ar atmosphere (p(H2O) = 2.34 kPa), which implied that BCI25, having good stability and sensitivity, is a
promising high-temperature humidity sensor.
PB  - Elsevier Ltd
PB  - Techna Group S.r.l.
T2  - Ceramics International
T1  - Electrical and sensing properties of indium-doped barium cerate
EP  - 15679
SP  - 15673
VL  - 49
DO  - 10.1016/j.ceramint.2023.01.159
ER  - 
@article{
author = "Malešević, Aleksandar and Radojković, Aleksandar and Žunić, Milan and Savic, Slavica and Perać, Sanja and Branković, Zorica and Branković, Goran",
year = "2023",
abstract = "Systematic analysis of electrical characteristics of BaCe0.75In0.25O3-δ (BCI25) sintered sample was performed in a
dry and a wet argon atmosphere in the 250 ◦C–700 ◦C temperature range. The water vapor sensing properties of
BCI25 porous film and its response and recovery times were investigated under different conditions of temperature
and water vapor concentration. The 30 μm thick film obtained from the powder calcined at 1050 ◦C
exhibited sensitivity comparable to that of the sintered sample with a significantly shorter response and recovery
times. While the sensitivity of the film gradually decreased with a decrease in partial pressure of water vapor (p
(H2O)), a noticeable sensitivity was still observed at p(H2O) of 200 Pa. Decrease in conductivity depended
logarithmically on the partial pressure of water with the slope of 0.52 that is close to the theoretical value. After
several cycles, the reusability test proved an almost unchanged ratio between the impedance value in the dry and
the wet Ar atmosphere (p(H2O) = 2.34 kPa), which implied that BCI25, having good stability and sensitivity, is a
promising high-temperature humidity sensor.",
publisher = "Elsevier Ltd, Techna Group S.r.l.",
journal = "Ceramics International",
title = "Electrical and sensing properties of indium-doped barium cerate",
pages = "15679-15673",
volume = "49",
doi = "10.1016/j.ceramint.2023.01.159"
}
Malešević, A., Radojković, A., Žunić, M., Savic, S., Perać, S., Branković, Z.,& Branković, G.. (2023). Electrical and sensing properties of indium-doped barium cerate. in Ceramics International
Elsevier Ltd., 49, 15673-15679.
https://doi.org/10.1016/j.ceramint.2023.01.159
Malešević A, Radojković A, Žunić M, Savic S, Perać S, Branković Z, Branković G. Electrical and sensing properties of indium-doped barium cerate. in Ceramics International. 2023;49:15673-15679.
doi:10.1016/j.ceramint.2023.01.159 .
Malešević, Aleksandar, Radojković, Aleksandar, Žunić, Milan, Savic, Slavica, Perać, Sanja, Branković, Zorica, Branković, Goran, "Electrical and sensing properties of indium-doped barium cerate" in Ceramics International, 49 (2023):15673-15679,
https://doi.org/10.1016/j.ceramint.2023.01.159 . .
1

Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy

Brdar, Sanja; Panic, Marko; Matavulj, Predrag; Stanković, Mira; Bartolić, Dragana; Sikoparija, Branko

(Springer Nature, 2023)

TY  - JOUR
AU  - Brdar, Sanja
AU  - Panic, Marko
AU  - Matavulj, Predrag
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Sikoparija, Branko
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1799
AB  - Pollen monitoring have become data-intensive in recent years as real-time detectors are deployed
to classify airborne pollen grains. Machine learning models with a focus on deep learning, have
an essential role in the pollen classifcation task. Within this study we developed an explainable
framework to unveil a deep learning model for pollen classifcation. Model works on data coming
from single particle detector (Rapid-E) that records for each particle optical fngerprint with scattered
light and laser induced fuorescence. Morphological properties of a particle are sensed with the
light scattering process, while chemical properties are encoded with fuorescence spectrum and
fuorescence lifetime induced by high-resolution laser. By utilizing these three data modalities,
scattering, spectrum, and lifetime, deep learning-based models with millions of parameters are
learned to distinguish diferent pollen classes, but a proper understanding of such a black-box model
decisions demands additional methods to employ. Our study provides the frst results of applied
explainable artifcial intelligence (xAI) methodology on the pollen classifcation model. Extracted
knowledge on the important features that attribute to the predicting particular pollen classes is
further examined from the perspective of domain knowledge and compared to available reference
data on pollen sizes, shape, and laboratory spectrofuorometer measurements.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy
IS  - 3205
VL  - 13
DO  - 10.1038/s41598-023-30064-6
ER  - 
@article{
author = "Brdar, Sanja and Panic, Marko and Matavulj, Predrag and Stanković, Mira and Bartolić, Dragana and Sikoparija, Branko",
year = "2023",
abstract = "Pollen monitoring have become data-intensive in recent years as real-time detectors are deployed
to classify airborne pollen grains. Machine learning models with a focus on deep learning, have
an essential role in the pollen classifcation task. Within this study we developed an explainable
framework to unveil a deep learning model for pollen classifcation. Model works on data coming
from single particle detector (Rapid-E) that records for each particle optical fngerprint with scattered
light and laser induced fuorescence. Morphological properties of a particle are sensed with the
light scattering process, while chemical properties are encoded with fuorescence spectrum and
fuorescence lifetime induced by high-resolution laser. By utilizing these three data modalities,
scattering, spectrum, and lifetime, deep learning-based models with millions of parameters are
learned to distinguish diferent pollen classes, but a proper understanding of such a black-box model
decisions demands additional methods to employ. Our study provides the frst results of applied
explainable artifcial intelligence (xAI) methodology on the pollen classifcation model. Extracted
knowledge on the important features that attribute to the predicting particular pollen classes is
further examined from the perspective of domain knowledge and compared to available reference
data on pollen sizes, shape, and laboratory spectrofuorometer measurements.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy",
number = "3205",
volume = "13",
doi = "10.1038/s41598-023-30064-6"
}
Brdar, S., Panic, M., Matavulj, P., Stanković, M., Bartolić, D.,& Sikoparija, B.. (2023). Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy. in Scientific Reports
Springer Nature., 13(3205).
https://doi.org/10.1038/s41598-023-30064-6
Brdar S, Panic M, Matavulj P, Stanković M, Bartolić D, Sikoparija B. Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy. in Scientific Reports. 2023;13(3205).
doi:10.1038/s41598-023-30064-6 .
Brdar, Sanja, Panic, Marko, Matavulj, Predrag, Stanković, Mira, Bartolić, Dragana, Sikoparija, Branko, "Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy" in Scientific Reports, 13, no. 3205 (2023),
https://doi.org/10.1038/s41598-023-30064-6 . .
9
3

Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies

Stanković, Mira; Prokopijević, Miloš; Sikoparija, Branko; Nedic, Nebojsa; Andric, Filip; Natalija, Polović; Natić, Maja; Radotić, Ksenija

(MDPI, 2023)

TY  - JOUR
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Sikoparija, Branko
AU  - Nedic, Nebojsa
AU  - Andric, Filip
AU  - Natalija, Polović
AU  - Natić, Maja
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1781
AB  - Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera)
colonies. This study aimed to find a promising marker in honey for the bee colony infestation level
using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of
the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and
phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level
was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the
infestation level was positively correlated with the phenolic spectral component (r = 0.656), which
was tentatively related to the phenolics in honey. No correlation was found between the diastase
activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in
honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level
of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.
PB  - MDPI
T2  - Foods
T1  - Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies
EP  - 13
SP  - 629
SP  - 1
VL  - 12
DO  - 10.3390/foods12030629
ER  - 
@article{
author = "Stanković, Mira and Prokopijević, Miloš and Sikoparija, Branko and Nedic, Nebojsa and Andric, Filip and Natalija, Polović and Natić, Maja and Radotić, Ksenija",
year = "2023",
abstract = "Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera)
colonies. This study aimed to find a promising marker in honey for the bee colony infestation level
using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of
the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and
phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level
was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the
infestation level was positively correlated with the phenolic spectral component (r = 0.656), which
was tentatively related to the phenolics in honey. No correlation was found between the diastase
activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in
honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level
of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.",
publisher = "MDPI",
journal = "Foods",
title = "Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies",
pages = "13-629-1",
volume = "12",
doi = "10.3390/foods12030629"
}
Stanković, M., Prokopijević, M., Sikoparija, B., Nedic, N., Andric, F., Natalija, P., Natić, M.,& Radotić, K.. (2023). Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods
MDPI., 12, 629-13.
https://doi.org/10.3390/foods12030629
Stanković M, Prokopijević M, Sikoparija B, Nedic N, Andric F, Natalija P, Natić M, Radotić K. Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods. 2023;12:629-13.
doi:10.3390/foods12030629 .
Stanković, Mira, Prokopijević, Miloš, Sikoparija, Branko, Nedic, Nebojsa, Andric, Filip, Natalija, Polović, Natić, Maja, Radotić, Ksenija, "Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies" in Foods, 12 (2023):629-13,
https://doi.org/10.3390/foods12030629 . .
1
2

KIT-5-Assisted Synthesis of Mesoporous SnO2 for High-Performance Humidity Sensors with a Swift Response/Recovery Speed

Vojisavljević, Katarina; Savić, Slavica M.; Počuča-Nešić, Milica; Hodžić, Aden; Kriechbaum, Manfred; Ribić, Vesna; Rečnik, Aleksander; Mitrović, Jelena; Branković, Goran; Djokic, Veljko

(MDPI, 2023)

TY  - JOUR
AU  - Vojisavljević, Katarina
AU  - Savić, Slavica M.
AU  - Počuča-Nešić, Milica
AU  - Hodžić, Aden
AU  - Kriechbaum, Manfred
AU  - Ribić, Vesna
AU  - Rečnik, Aleksander
AU  - Mitrović, Jelena
AU  - Branković, Goran
AU  - Djokic, Veljko
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1860
AB  - Developing highly efficient semiconductor metal oxide (SMOX) sensors capable of accurate and fast responses to environmental humidity is still a challenging task. In addition to a not so pronounced sensitivity to relative humidity change, most of the SMOXs cannot meet the criteria of real-time humidity sensing due to their long response/recovery time. The way to tackle this problem is to control adsorption/desorption processes, i.e., water-vapor molecular dynamics, over the sensor’s active layer through the powder and pore morphology design. With this in mind, a KIT-5-mediated synthesis was used to achieve mesoporous tin (IV) oxide replica (SnO2-R) with controlled pore size and ordering through template inversion and compared with a sol-gel synthesized powder (SnO2-SG). Unlike SnO2-SG, SnO2-R possessed a high specific surface area and quite an open pore structure, similar to the KIT-5, as observed by TEM, BET and SWAXS analyses. According to TEM, SnO2-R consisted of fine-grained globular particles and some percent of exaggerated, grown twinned crystals. The distinctive morphology of the SnO2-R-based sensor, with its specific pore structure and an increased number of oxygen-related defects associated with the powder preparation process and detected at the sensor surface by XPS analysis, contributed to excellent humidity sensing performances at room temperature, comprised of a low hysteresis error (3.7%), sensitivity of 406.8 kΩ/RH% and swift response/recovery speed (4 s/6 s).
PB  - MDPI
T2  - Molecules
T1  - KIT-5-Assisted Synthesis of Mesoporous SnO2 for High-Performance Humidity Sensors with a Swift Response/Recovery Speed
IS  - 4
SP  - 1754
VL  - 28
DO  - 10.3390/molecules28041754
ER  - 
@article{
author = "Vojisavljević, Katarina and Savić, Slavica M. and Počuča-Nešić, Milica and Hodžić, Aden and Kriechbaum, Manfred and Ribić, Vesna and Rečnik, Aleksander and Mitrović, Jelena and Branković, Goran and Djokic, Veljko",
year = "2023",
abstract = "Developing highly efficient semiconductor metal oxide (SMOX) sensors capable of accurate and fast responses to environmental humidity is still a challenging task. In addition to a not so pronounced sensitivity to relative humidity change, most of the SMOXs cannot meet the criteria of real-time humidity sensing due to their long response/recovery time. The way to tackle this problem is to control adsorption/desorption processes, i.e., water-vapor molecular dynamics, over the sensor’s active layer through the powder and pore morphology design. With this in mind, a KIT-5-mediated synthesis was used to achieve mesoporous tin (IV) oxide replica (SnO2-R) with controlled pore size and ordering through template inversion and compared with a sol-gel synthesized powder (SnO2-SG). Unlike SnO2-SG, SnO2-R possessed a high specific surface area and quite an open pore structure, similar to the KIT-5, as observed by TEM, BET and SWAXS analyses. According to TEM, SnO2-R consisted of fine-grained globular particles and some percent of exaggerated, grown twinned crystals. The distinctive morphology of the SnO2-R-based sensor, with its specific pore structure and an increased number of oxygen-related defects associated with the powder preparation process and detected at the sensor surface by XPS analysis, contributed to excellent humidity sensing performances at room temperature, comprised of a low hysteresis error (3.7%), sensitivity of 406.8 kΩ/RH% and swift response/recovery speed (4 s/6 s).",
publisher = "MDPI",
journal = "Molecules",
title = "KIT-5-Assisted Synthesis of Mesoporous SnO2 for High-Performance Humidity Sensors with a Swift Response/Recovery Speed",
number = "4",
pages = "1754",
volume = "28",
doi = "10.3390/molecules28041754"
}
Vojisavljević, K., Savić, S. M., Počuča-Nešić, M., Hodžić, A., Kriechbaum, M., Ribić, V., Rečnik, A., Mitrović, J., Branković, G.,& Djokic, V.. (2023). KIT-5-Assisted Synthesis of Mesoporous SnO2 for High-Performance Humidity Sensors with a Swift Response/Recovery Speed. in Molecules
MDPI., 28(4), 1754.
https://doi.org/10.3390/molecules28041754
Vojisavljević K, Savić SM, Počuča-Nešić M, Hodžić A, Kriechbaum M, Ribić V, Rečnik A, Mitrović J, Branković G, Djokic V. KIT-5-Assisted Synthesis of Mesoporous SnO2 for High-Performance Humidity Sensors with a Swift Response/Recovery Speed. in Molecules. 2023;28(4):1754.
doi:10.3390/molecules28041754 .
Vojisavljević, Katarina, Savić, Slavica M., Počuča-Nešić, Milica, Hodžić, Aden, Kriechbaum, Manfred, Ribić, Vesna, Rečnik, Aleksander, Mitrović, Jelena, Branković, Goran, Djokic, Veljko, "KIT-5-Assisted Synthesis of Mesoporous SnO2 for High-Performance Humidity Sensors with a Swift Response/Recovery Speed" in Molecules, 28, no. 4 (2023):1754,
https://doi.org/10.3390/molecules28041754 . .
3

Microstructural, Thermoelectric and Mechanical Properties of Cu Substituted NaCo2O4

Perać, Sanja; M.Savić, Slavica; Branković, Zorica; Bernik, Slavko; Radojković, Aleksandar; Kojić, Sanja; Vasiljević, Dragana; Branković, Goran

(MDPI, 2022)

TY  - JOUR
AU  - Perać, Sanja
AU  - M.Savić, Slavica
AU  - Branković, Zorica
AU  - Bernik, Slavko
AU  - Radojković, Aleksandar
AU  - Kojić, Sanja
AU  - Vasiljević, Dragana
AU  - Branković, Goran
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1561
AB  - Polycrystalline samples of NaCo2−xCuxO4 (x = 0, 0.01, 0.03, 0.05) were obtained from powder precursors synthesized by a mechanochemically assisted solid-state reaction method (MASSR) and a citric acid complex method (CAC). Ceramic samples were prepared by pressing into disc-shaped pellets and subsequently sintering at 880 °C in an argon atmosphere. Effects of low concentrations of Cu doping and the above-mentioned synthesis procedures on the thermoelectric and mechanical properties were observed. The electrical resistivity (ρ), the thermal conductivity (κ) and the Seebeck coefficient (S) were measured simultaneously in the temperature gradient (ΔT) between the hot and cold side of the sample, and the figure of merit (ZT) was subsequently calculated. The ZT of the CAC samples was higher compared with the MASSR samples. The highest ZT value of 0.061 at ΔT = 473 K was obtained for the sample with 5 mol% of Cu prepared by the CAC method. The CAC samples showed better mechanical properties compared to the MASSR samples due to the higher hardness of the CAC samples which is a consequence of homogeneous microstructure and higher density obtained during sintering of these samples. The results confirmed that, besides the concentration of Cu, the synthesis procedure considerably affected the thermoelectric and mechanical properties of NaCo2O4 (NCO) ceramics.
PB  - MDPI
T2  - Materials
T1  - Microstructural, Thermoelectric and Mechanical Properties of Cu Substituted NaCo2O4
SP  - 4470
VL  - 15
DO  - 10.3390/ma15134470
ER  - 
@article{
author = "Perać, Sanja and M.Savić, Slavica and Branković, Zorica and Bernik, Slavko and Radojković, Aleksandar and Kojić, Sanja and Vasiljević, Dragana and Branković, Goran",
year = "2022",
abstract = "Polycrystalline samples of NaCo2−xCuxO4 (x = 0, 0.01, 0.03, 0.05) were obtained from powder precursors synthesized by a mechanochemically assisted solid-state reaction method (MASSR) and a citric acid complex method (CAC). Ceramic samples were prepared by pressing into disc-shaped pellets and subsequently sintering at 880 °C in an argon atmosphere. Effects of low concentrations of Cu doping and the above-mentioned synthesis procedures on the thermoelectric and mechanical properties were observed. The electrical resistivity (ρ), the thermal conductivity (κ) and the Seebeck coefficient (S) were measured simultaneously in the temperature gradient (ΔT) between the hot and cold side of the sample, and the figure of merit (ZT) was subsequently calculated. The ZT of the CAC samples was higher compared with the MASSR samples. The highest ZT value of 0.061 at ΔT = 473 K was obtained for the sample with 5 mol% of Cu prepared by the CAC method. The CAC samples showed better mechanical properties compared to the MASSR samples due to the higher hardness of the CAC samples which is a consequence of homogeneous microstructure and higher density obtained during sintering of these samples. The results confirmed that, besides the concentration of Cu, the synthesis procedure considerably affected the thermoelectric and mechanical properties of NaCo2O4 (NCO) ceramics.",
publisher = "MDPI",
journal = "Materials",
title = "Microstructural, Thermoelectric and Mechanical Properties of Cu Substituted NaCo2O4",
pages = "4470",
volume = "15",
doi = "10.3390/ma15134470"
}
Perać, S., M.Savić, S., Branković, Z., Bernik, S., Radojković, A., Kojić, S., Vasiljević, D.,& Branković, G.. (2022). Microstructural, Thermoelectric and Mechanical Properties of Cu Substituted NaCo2O4. in Materials
MDPI., 15, 4470.
https://doi.org/10.3390/ma15134470
Perać S, M.Savić S, Branković Z, Bernik S, Radojković A, Kojić S, Vasiljević D, Branković G. Microstructural, Thermoelectric and Mechanical Properties of Cu Substituted NaCo2O4. in Materials. 2022;15:4470.
doi:10.3390/ma15134470 .
Perać, Sanja, M.Savić, Slavica, Branković, Zorica, Bernik, Slavko, Radojković, Aleksandar, Kojić, Sanja, Vasiljević, Dragana, Branković, Goran, "Microstructural, Thermoelectric and Mechanical Properties of Cu Substituted NaCo2O4" in Materials, 15 (2022):4470,
https://doi.org/10.3390/ma15134470 . .
1

Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells

Bulajic, Dragica V.; Drljaca, Jovana; Capo, Ivan; Savić, Slavica M.; Vojisavljević, Katarina; Hodzic, Aden; Sekulic, Slobodan; Bajkin, Branislav V.

(Wiley, Hoboken, 2021)

TY  - JOUR
AU  - Bulajic, Dragica V.
AU  - Drljaca, Jovana
AU  - Capo, Ivan
AU  - Savić, Slavica M.
AU  - Vojisavljević, Katarina
AU  - Hodzic, Aden
AU  - Sekulic, Slobodan
AU  - Bajkin, Branislav V.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1474
AB  - In the present work, a biomaterial (SBA-16/HA) based on the growth of hydroxyapatite (HA) particles within an organized silica structure SBA-16 (Santa Barbara Amorphous-16) was developed to evaluate its application to act as a porous microenvironment promoting attachment and viability of human dental pulp stem cells of healthy deciduous teeth (SHED). First, SHED were isolated and their phenotypes were evaluated by flow cytometry. The samples of SBA-16/HA were characterized by X-ray diffraction (XRD), small and wide angle X-ray scattering (SWAXS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) equipped with energy dispersive spectra detector (EDS). Afterward, cells were cultured in the eluates of the above-mentioned biomaterial aged for 24 hr, 7. and 14 days. Bio-Oss (R) and dentin particles are involved for comparison and cells are cultured in the eluates of these two materials also. Thiazolyl Blue Tetrazolium bromide assay-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay) was used for the determination of cell viability. The results obtained by all aforementioned characterization methods of SBA-16/HA, revealed a uniform spherical mesoporous structure, an intrinsic characteristic of this material. This material displayed excellent biocompatibility on SHEDs, and even proliferative potential, indicating that SBA-16/HA could potentially serve as a suitable substrate for bone regeneration. Contrary to SBA-16/HA, dentin particles showed low cytotoxicity at all time points, compared to control and Bio-Oss (R) groups. Our results substantiate the idea that SBA-16/HA has a beneficial effect on SHEDs, thus paving the way toward developing new material for bone replacement.
PB  - Wiley, Hoboken
T2  - Microscopy Research and Technique
T1  - Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells
DO  - 10.1002/jemt.24017
ER  - 
@article{
author = "Bulajic, Dragica V. and Drljaca, Jovana and Capo, Ivan and Savić, Slavica M. and Vojisavljević, Katarina and Hodzic, Aden and Sekulic, Slobodan and Bajkin, Branislav V.",
year = "2021",
abstract = "In the present work, a biomaterial (SBA-16/HA) based on the growth of hydroxyapatite (HA) particles within an organized silica structure SBA-16 (Santa Barbara Amorphous-16) was developed to evaluate its application to act as a porous microenvironment promoting attachment and viability of human dental pulp stem cells of healthy deciduous teeth (SHED). First, SHED were isolated and their phenotypes were evaluated by flow cytometry. The samples of SBA-16/HA were characterized by X-ray diffraction (XRD), small and wide angle X-ray scattering (SWAXS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) equipped with energy dispersive spectra detector (EDS). Afterward, cells were cultured in the eluates of the above-mentioned biomaterial aged for 24 hr, 7. and 14 days. Bio-Oss (R) and dentin particles are involved for comparison and cells are cultured in the eluates of these two materials also. Thiazolyl Blue Tetrazolium bromide assay-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay) was used for the determination of cell viability. The results obtained by all aforementioned characterization methods of SBA-16/HA, revealed a uniform spherical mesoporous structure, an intrinsic characteristic of this material. This material displayed excellent biocompatibility on SHEDs, and even proliferative potential, indicating that SBA-16/HA could potentially serve as a suitable substrate for bone regeneration. Contrary to SBA-16/HA, dentin particles showed low cytotoxicity at all time points, compared to control and Bio-Oss (R) groups. Our results substantiate the idea that SBA-16/HA has a beneficial effect on SHEDs, thus paving the way toward developing new material for bone replacement.",
publisher = "Wiley, Hoboken",
journal = "Microscopy Research and Technique",
title = "Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells",
doi = "10.1002/jemt.24017"
}
Bulajic, D. V., Drljaca, J., Capo, I., Savić, S. M., Vojisavljević, K., Hodzic, A., Sekulic, S.,& Bajkin, B. V.. (2021). Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells. in Microscopy Research and Technique
Wiley, Hoboken..
https://doi.org/10.1002/jemt.24017
Bulajic DV, Drljaca J, Capo I, Savić SM, Vojisavljević K, Hodzic A, Sekulic S, Bajkin BV. Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells. in Microscopy Research and Technique. 2021;.
doi:10.1002/jemt.24017 .
Bulajic, Dragica V., Drljaca, Jovana, Capo, Ivan, Savić, Slavica M., Vojisavljević, Katarina, Hodzic, Aden, Sekulic, Slobodan, Bajkin, Branislav V., "Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells" in Microscopy Research and Technique (2021),
https://doi.org/10.1002/jemt.24017 . .
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