TY  - JOUR
AU  - Pavlović, Nikola M
AU  - Maksimović, Vuk
AU  - Dragišić Maksimović, Jelena
AU  - Orem, William H
AU  - Tatu, Calin A
AU  - Lerch, Harry
AU  - Bunnell, Joseph E
AU  - Kostic, Emina N
AU  - Szilagyi, Diana N
AU  - Paunescu, Virgil
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/686
AB  - Aristolochic acids (AAs) are nephrotoxic and carcinogenic derivatives found in several Aristolochia species. To date, the toxicity of AAs has been inferred only from the effects observed in patients suffering from a kidney disease called "aristolochic acid nephropathy" (AAN, formerly known as "Chinese herbs nephropathy"). More recently, the chronic poisoning with Aristolochia seeds has been considered to be the main cause of Balkan endemic nephropathy, another form of chronic renal failure resembling AAN. So far, it was assumed that AAs can enter the human food chain only through ethnobotanical use (intentional or accidental) of herbs containing self-produced AAs. We hypothesized that the roots of some crops growing in fields where Aristolochia species grew over several seasons may take up certain amounts of AAs from the soil, and thus become a secondary source of food poisoning. To verify this possibility, maize plant (Zea mays) and cucumber (Cucumis sativus) were used as a model to substantiate the possible significance of naturally occurring AAs' root uptake in food chain contamination. This study showed that the roots of maize plant and cucumber are capable of absorbing AAs from nutrient solution, consequently producing strong peaks on ultraviolet HPLC chromatograms of plant extracts. This uptake resulted in even higher concentrations of AAs in the roots compared to the nutrient solutions. To further validate the measurement of AA content in the root material, we also measured their concentrations in nutrient solutions before and after the plant treatment. Decreased concentrations of both AAI and AAII were found in nutrient solutions after plant growth. During this short-term experiment, there were much lower concentrations of AAs in the leaves than in the roots. The question is whether these plants are capable of transferring significant amounts of AAs from the roots into edible parts of the plant during prolonged experiments.
PB  - Springer, Dordrecht
T2  - Environmental Geochemistry and Health
T1  - Possible health impacts of naturally occurring uptake of aristolochic acids by maize and cucumber roots: links to the etiology of endemic (Balkan) nephropathy
EP  - 226
IS  - 2
SP  - 215
VL  - 35
DO  - 10.1007/s10653-012-9477-8
ER  - 

@article{
author = "Pavlović, Nikola M and Maksimović, Vuk and Dragišić Maksimović, Jelena and Orem, William H and Tatu, Calin A and Lerch, Harry and Bunnell, Joseph E and Kostic, Emina N and Szilagyi, Diana N and Paunescu, Virgil",
year = "2013",
abstract = "Aristolochic acids (AAs) are nephrotoxic and carcinogenic derivatives found in several Aristolochia species. To date, the toxicity of AAs has been inferred only from the effects observed in patients suffering from a kidney disease called "aristolochic acid nephropathy" (AAN, formerly known as "Chinese herbs nephropathy"). More recently, the chronic poisoning with Aristolochia seeds has been considered to be the main cause of Balkan endemic nephropathy, another form of chronic renal failure resembling AAN. So far, it was assumed that AAs can enter the human food chain only through ethnobotanical use (intentional or accidental) of herbs containing self-produced AAs. We hypothesized that the roots of some crops growing in fields where Aristolochia species grew over several seasons may take up certain amounts of AAs from the soil, and thus become a secondary source of food poisoning. To verify this possibility, maize plant (Zea mays) and cucumber (Cucumis sativus) were used as a model to substantiate the possible significance of naturally occurring AAs' root uptake in food chain contamination. This study showed that the roots of maize plant and cucumber are capable of absorbing AAs from nutrient solution, consequently producing strong peaks on ultraviolet HPLC chromatograms of plant extracts. This uptake resulted in even higher concentrations of AAs in the roots compared to the nutrient solutions. To further validate the measurement of AA content in the root material, we also measured their concentrations in nutrient solutions before and after the plant treatment. Decreased concentrations of both AAI and AAII were found in nutrient solutions after plant growth. During this short-term experiment, there were much lower concentrations of AAs in the leaves than in the roots. The question is whether these plants are capable of transferring significant amounts of AAs from the roots into edible parts of the plant during prolonged experiments.",
publisher = "Springer, Dordrecht",
journal = "Environmental Geochemistry and Health",
title = "Possible health impacts of naturally occurring uptake of aristolochic acids by maize and cucumber roots: links to the etiology of endemic (Balkan) nephropathy",
pages = "226-215",
number = "2",
volume = "35",
doi = "10.1007/s10653-012-9477-8"
}

Pavlović, N. M., Maksimović, V., Dragišić Maksimović, J., Orem, W. H., Tatu, C. A., Lerch, H., Bunnell, J. E., Kostic, E. N., Szilagyi, D. N.,& Paunescu, V.. (2013). Possible health impacts of naturally occurring uptake of aristolochic acids by maize and cucumber roots: links to the etiology of endemic (Balkan) nephropathy. in Environmental Geochemistry and Health
Springer, Dordrecht., 35(2), 215-226.
https://doi.org/10.1007/s10653-012-9477-8

Pavlović NM, Maksimović V, Dragišić Maksimović J, Orem WH, Tatu CA, Lerch H, Bunnell JE, Kostic EN, Szilagyi DN, Paunescu V. Possible health impacts of naturally occurring uptake of aristolochic acids by maize and cucumber roots: links to the etiology of endemic (Balkan) nephropathy. in Environmental Geochemistry and Health. 2013;35(2):215-226.
doi:10.1007/s10653-012-9477-8 .

Pavlović, Nikola M, Maksimović, Vuk, Dragišić Maksimović, Jelena, Orem, William H, Tatu, Calin A, Lerch, Harry, Bunnell, Joseph E, Kostic, Emina N, Szilagyi, Diana N, Paunescu, Virgil, "Possible health impacts of naturally occurring uptake of aristolochic acids by maize and cucumber roots: links to the etiology of endemic (Balkan) nephropathy" in Environmental Geochemistry and Health, 35, no. 2 (2013):215-226,
https://doi.org/10.1007/s10653-012-9477-8 . .

TY  - JOUR
AU  - Stajić, Mirjana
AU  - Kukavica, Biljana
AU  - Vukojevic, Jelena
AU  - Ćilerdžić, Jasmina
AU  - Veljovic Jovanovic, Sonja
AU  - Duletić-Laušević, Sonja
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3131
AB  - The purpose of this study was to resolve the question of whether various nitrogen sources and concentrations affect characteristics of selected G. lucidum ligninolytic enzymes participating in wheat straw fermentation. This is the first study reporting the presence of versatile peroxidase activity in crude extract of G. lucidum culture, as well as isoforms profile of Mn-oxidizing peroxidases. NH4NO3 was the optimum nitrogen source for laccase and Mn-dependent peroxidase activity, while peptone was the optimum one for versatile peroxidase activity. Four bands with laccase activity were obtained by native PAGE and IEF separations from medium enriched with inorganic nitrogen source, and only two bands from medium containing organic source. Medium composition was not shown to affect isoenzyme patterns of Mn-oxidizing peroxidases. Four isoforms of Mn-dependent peroxidase and three of versatile peroxidase were obtained on native PAGE. By IEF separation, five isoforms of Mn-dependent peroxidase and only two of versatile peroxidase were observed. The results demonstrated that G. lucidum has potential for mineralization and transformation of various agricultural residues and should take more significant participation in large-scale biotechnological processes.
PB  - NC State University
T2  - BioResources
T1  - WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM
EP  - 2373
IS  - 5
SP  - 2362
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3131
ER  - 

@article{
author = "Stajić, Mirjana and Kukavica, Biljana and Vukojevic, Jelena and Ćilerdžić, Jasmina and Veljovic Jovanovic, Sonja and Duletić-Laušević, Sonja",
year = "2010",
abstract = "The purpose of this study was to resolve the question of whether various nitrogen sources and concentrations affect characteristics of selected G. lucidum ligninolytic enzymes participating in wheat straw fermentation. This is the first study reporting the presence of versatile peroxidase activity in crude extract of G. lucidum culture, as well as isoforms profile of Mn-oxidizing peroxidases. NH4NO3 was the optimum nitrogen source for laccase and Mn-dependent peroxidase activity, while peptone was the optimum one for versatile peroxidase activity. Four bands with laccase activity were obtained by native PAGE and IEF separations from medium enriched with inorganic nitrogen source, and only two bands from medium containing organic source. Medium composition was not shown to affect isoenzyme patterns of Mn-oxidizing peroxidases. Four isoforms of Mn-dependent peroxidase and three of versatile peroxidase were obtained on native PAGE. By IEF separation, five isoforms of Mn-dependent peroxidase and only two of versatile peroxidase were observed. The results demonstrated that G. lucidum has potential for mineralization and transformation of various agricultural residues and should take more significant participation in large-scale biotechnological processes.",
publisher = "NC State University",
journal = "BioResources",
title = "WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM",
pages = "2373-2362",
number = "5",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3131"
}

Stajić, M., Kukavica, B., Vukojevic, J., Ćilerdžić, J., Veljovic Jovanovic, S.,& Duletić-Laušević, S.. (2010). WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM. in BioResources
NC State University., 4(5), 2362-2373.
https://hdl.handle.net/21.15107/rcub_rimsi_3131

Stajić M, Kukavica B, Vukojevic J, Ćilerdžić J, Veljovic Jovanovic S, Duletić-Laušević S. WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM. in BioResources. 2010;4(5):2362-2373.
https://hdl.handle.net/21.15107/rcub_rimsi_3131 .

Stajić, Mirjana, Kukavica, Biljana, Vukojevic, Jelena, Ćilerdžić, Jasmina, Veljovic Jovanovic, Sonja, Duletić-Laušević, Sonja, "WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM" in BioResources, 4, no. 5 (2010):2362-2373,
https://hdl.handle.net/21.15107/rcub_rimsi_3131 .

TY  - JOUR
AU  - Morina, Filis
AU  - Jovanović, Ljubinko
AU  - Mojović, Miloš
AU  - Vidović, Marija
AU  - Panković, Dejana M
AU  - Veljović-Jovanović, Sonja
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/379
AB  - Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn2+. Reactive oxygen species (ROS) accumulation was demonstrated by the fluorescent probe H(2)DCFDA and EPR measurements. The extent of zinc-induced oxidative damage was assessed by measuring the level of protein carbonylation. Activities and isoform profiles of some antioxidant enzymes and the changes in ascorbate and total phenolic contents of leaves and roots were determined. Stunted growth because of zinc accumulation, preferentially in the roots, was accompanied by H2O2 production in the leaf and root apoplasts. Increased EPR signals of the endogenous oxidant quinhydrone, center dot CH3 and center dot OH, were found in the cell walls of zinc-treated plants. The activities of the antioxidative enzymes ascorbate peroxidase (APX) (EC 1.11.1.11), soluble superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD), (EC 1.11.1.7) and monodehydroascorbate reductase (EC 1.6.5.4) were increased; those of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and ascorbate oxidase (AAO) (EC 1.10.3.3) were decreased with zinc treatment. Zinc induced a cell-wall-bound SOD isoform in both organs. Leaves accumulated more ascorbate and phenolics in comparison to roots. We propose a mechanism for zinc-promoted oxidative stress in V. thapsus L. through the generation of charge transfer complexes and quinhydrone because of phenoxyl radical stabilisation by Zn2+ in the cell wall. Our results suggest that the SOD and APX responses are mediated by ROS accumulation in the apoplast. The importance of the POD/Phe/AA (ascorbic acid) scavenging system in the apoplast is also discussed.
PB  - Wiley, Hoboken
T2  - Physiologia Plantarum
T1  - Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall
EP  - 224
IS  - 3
SP  - 209
VL  - 140
DO  - 10.1111/j.1399-3054.2010.01399.x
ER  - 

@article{
author = "Morina, Filis and Jovanović, Ljubinko and Mojović, Miloš and Vidović, Marija and Panković, Dejana M and Veljović-Jovanović, Sonja",
year = "2010",
abstract = "Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn2+. Reactive oxygen species (ROS) accumulation was demonstrated by the fluorescent probe H(2)DCFDA and EPR measurements. The extent of zinc-induced oxidative damage was assessed by measuring the level of protein carbonylation. Activities and isoform profiles of some antioxidant enzymes and the changes in ascorbate and total phenolic contents of leaves and roots were determined. Stunted growth because of zinc accumulation, preferentially in the roots, was accompanied by H2O2 production in the leaf and root apoplasts. Increased EPR signals of the endogenous oxidant quinhydrone, center dot CH3 and center dot OH, were found in the cell walls of zinc-treated plants. The activities of the antioxidative enzymes ascorbate peroxidase (APX) (EC 1.11.1.11), soluble superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD), (EC 1.11.1.7) and monodehydroascorbate reductase (EC 1.6.5.4) were increased; those of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and ascorbate oxidase (AAO) (EC 1.10.3.3) were decreased with zinc treatment. Zinc induced a cell-wall-bound SOD isoform in both organs. Leaves accumulated more ascorbate and phenolics in comparison to roots. We propose a mechanism for zinc-promoted oxidative stress in V. thapsus L. through the generation of charge transfer complexes and quinhydrone because of phenoxyl radical stabilisation by Zn2+ in the cell wall. Our results suggest that the SOD and APX responses are mediated by ROS accumulation in the apoplast. The importance of the POD/Phe/AA (ascorbic acid) scavenging system in the apoplast is also discussed.",
publisher = "Wiley, Hoboken",
journal = "Physiologia Plantarum",
title = "Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall",
pages = "224-209",
number = "3",
volume = "140",
doi = "10.1111/j.1399-3054.2010.01399.x"
}

Morina, F., Jovanović, L., Mojović, M., Vidović, M., Panković, D. M.,& Veljović-Jovanović, S.. (2010). Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall. in Physiologia Plantarum
Wiley, Hoboken., 140(3), 209-224.
https://doi.org/10.1111/j.1399-3054.2010.01399.x

Morina F, Jovanović L, Mojović M, Vidović M, Panković DM, Veljović-Jovanović S. Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall. in Physiologia Plantarum. 2010;140(3):209-224.
doi:10.1111/j.1399-3054.2010.01399.x .

Morina, Filis, Jovanović, Ljubinko, Mojović, Miloš, Vidović, Marija, Panković, Dejana M, Veljović-Jovanović, Sonja, "Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall" in Physiologia Plantarum, 140, no. 3 (2010):209-224,
https://doi.org/10.1111/j.1399-3054.2010.01399.x . .

TY  - JOUR
AU  - Nestorović, Jasmina M
AU  - Misic, Danijela
AU  - Siler, Branislav
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ciric, Ana D
AU  - Maksimović, Vuk
AU  - Grubisic, Dragoljub V
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/372
AB  - Rapid micropropagation of Nepeta rtanjensis, N. sibirica and N. nervosa was performed. Qualitative and quantitative nepetalactone content in methanol extracts of in vitro grown plants was analysed by reverse-phase HPLC coupled with UV and MS detection. Only trans,cis-nepetalactone was detected in shoots of N. rtanjensis, while cis,trans-nepetalactone stereoisomer was present in N. sibirica. No nepetalactone was observed in shoots of N. nervosa. The antimicrobial activity of methanol extracts, against eight bacterial and eight fungal species, was evaluated. All the tested extracts showed significant antibacterial and strong antifungal activity. However, N. rtanjensis extract exhibited the best antimicrobial potential.
PB  - Elsevier, Amsterdam
T2  - Fitoterapia
T1  - Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity
EP  - 626
IS  - 6
SP  - 621
VL  - 81
DO  - 10.1016/j.fitote.2010.03.007
ER  - 

@article{
author = "Nestorović, Jasmina M and Misic, Danijela and Siler, Branislav and Soković, Marina and Glamočlija, Jasmina and Ciric, Ana D and Maksimović, Vuk and Grubisic, Dragoljub V",
year = "2010",
abstract = "Rapid micropropagation of Nepeta rtanjensis, N. sibirica and N. nervosa was performed. Qualitative and quantitative nepetalactone content in methanol extracts of in vitro grown plants was analysed by reverse-phase HPLC coupled with UV and MS detection. Only trans,cis-nepetalactone was detected in shoots of N. rtanjensis, while cis,trans-nepetalactone stereoisomer was present in N. sibirica. No nepetalactone was observed in shoots of N. nervosa. The antimicrobial activity of methanol extracts, against eight bacterial and eight fungal species, was evaluated. All the tested extracts showed significant antibacterial and strong antifungal activity. However, N. rtanjensis extract exhibited the best antimicrobial potential.",
publisher = "Elsevier, Amsterdam",
journal = "Fitoterapia",
title = "Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity",
pages = "626-621",
number = "6",
volume = "81",
doi = "10.1016/j.fitote.2010.03.007"
}

Nestorović, J. M., Misic, D., Siler, B., Soković, M., Glamočlija, J., Ciric, A. D., Maksimović, V.,& Grubisic, D. V.. (2010). Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity. in Fitoterapia
Elsevier, Amsterdam., 81(6), 621-626.
https://doi.org/10.1016/j.fitote.2010.03.007

Nestorović JM, Misic D, Siler B, Soković M, Glamočlija J, Ciric AD, Maksimović V, Grubisic DV. Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity. in Fitoterapia. 2010;81(6):621-626.
doi:10.1016/j.fitote.2010.03.007 .

Nestorović, Jasmina M, Misic, Danijela, Siler, Branislav, Soković, Marina, Glamočlija, Jasmina, Ciric, Ana D, Maksimović, Vuk, Grubisic, Dragoljub V, "Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity" in Fitoterapia, 81, no. 6 (2010):621-626,
https://doi.org/10.1016/j.fitote.2010.03.007 . .

TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Veljović-Jovanović, Sonja
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Pajic, Zorica
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/399
AB  - Silk of some maize genotypes contains a high level of phenolics that undergo enzymatic oxidation to form quinones, which condense among themselves or with proteins to form brown pigments. Two phenolic oxidizing enzymes, peroxidase (POD; EC 1.11.1.7) and polyphenol oxidase (PPO; EC 1.10.3.1), from maize (Zea mays L.) silk were characterised with respect to their preferred substrate, different isoforms and specific effectors. One browning silk sample with high, and two non-browning samples with low phenolic content were investigated. Although POD oxidizes a wide range of phenolic substrates in vitro, its activity rate was independent of silk phenolic content. PPO activity, detected with o-diphenolic substrates, was abundant only in browning silk, and low or absent in non-browning silk. Pollination increased POD but not PPO activity. Isoelectric-focusing (IEF) and specific staining for POD and PPO showed a high degree of polymorphism that varied with silk origin. The IEF pattern of POD revealed a number of anionic and several cationic isoenzymes, with the most pronounced having neutral pI 7 and a basic isoform with pI 10. Detected isoforms of PPO were anionic, except for one neutral form found only in browning silk, and occupied positions different from those of POD. Different inhibitory effects of NaN3, EDTA, KCN, and L-cysteine, as well as different impacts of a variety of cations on the oxidation of chlorogenic acid, mediated by PPO or POD, were detected. The findings are discussed in terms of a possible roles of these enzymes in defence and pollination.
PB  - Wiley-Blackwell, Hoboken
T2  - Plant Biology
T1  - Characterisation of phenol oxidase and peroxidase from maize silk
EP  - 413
IS  - 3
SP  - 406
VL  - 12
DO  - 10.1111/j.1438-8677.2009.00237.x
ER  - 

@article{
author = "Hadzi-Tasković Sukalović, Vesna and Veljović-Jovanović, Sonja and Dragišić Maksimović, Jelena and Maksimović, Vuk and Pajic, Zorica",
year = "2010",
abstract = "Silk of some maize genotypes contains a high level of phenolics that undergo enzymatic oxidation to form quinones, which condense among themselves or with proteins to form brown pigments. Two phenolic oxidizing enzymes, peroxidase (POD; EC 1.11.1.7) and polyphenol oxidase (PPO; EC 1.10.3.1), from maize (Zea mays L.) silk were characterised with respect to their preferred substrate, different isoforms and specific effectors. One browning silk sample with high, and two non-browning samples with low phenolic content were investigated. Although POD oxidizes a wide range of phenolic substrates in vitro, its activity rate was independent of silk phenolic content. PPO activity, detected with o-diphenolic substrates, was abundant only in browning silk, and low or absent in non-browning silk. Pollination increased POD but not PPO activity. Isoelectric-focusing (IEF) and specific staining for POD and PPO showed a high degree of polymorphism that varied with silk origin. The IEF pattern of POD revealed a number of anionic and several cationic isoenzymes, with the most pronounced having neutral pI 7 and a basic isoform with pI 10. Detected isoforms of PPO were anionic, except for one neutral form found only in browning silk, and occupied positions different from those of POD. Different inhibitory effects of NaN3, EDTA, KCN, and L-cysteine, as well as different impacts of a variety of cations on the oxidation of chlorogenic acid, mediated by PPO or POD, were detected. The findings are discussed in terms of a possible roles of these enzymes in defence and pollination.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Plant Biology",
title = "Characterisation of phenol oxidase and peroxidase from maize silk",
pages = "413-406",
number = "3",
volume = "12",
doi = "10.1111/j.1438-8677.2009.00237.x"
}

Hadzi-Tasković Sukalović, V., Veljović-Jovanović, S., Dragišić Maksimović, J., Maksimović, V.,& Pajic, Z.. (2010). Characterisation of phenol oxidase and peroxidase from maize silk. in Plant Biology
Wiley-Blackwell, Hoboken., 12(3), 406-413.
https://doi.org/10.1111/j.1438-8677.2009.00237.x

Hadzi-Tasković Sukalović V, Veljović-Jovanović S, Dragišić Maksimović J, Maksimović V, Pajic Z. Characterisation of phenol oxidase and peroxidase from maize silk. in Plant Biology. 2010;12(3):406-413.
doi:10.1111/j.1438-8677.2009.00237.x .

Hadzi-Tasković Sukalović, Vesna, Veljović-Jovanović, Sonja, Dragišić Maksimović, Jelena, Maksimović, Vuk, Pajic, Zorica, "Characterisation of phenol oxidase and peroxidase from maize silk" in Plant Biology, 12, no. 3 (2010):406-413,
https://doi.org/10.1111/j.1438-8677.2009.00237.x . .

TY  - JOUR
AU  - Vuletic, Mirjana M
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Marković, Ksenija
AU  - Dragišić Maksimović, Jelena
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/390
AB  - The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 (-)) or both nitrogen forms, nitrate and ammonium (NH4 (+)/NO3 (-)). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 (+)/NO3 (-) in comparison with NO3 (-) grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 (+)/NO3 (-) treatment. The PEG treatment decreased enzyme activities in NH4 (+)/NO3 (-) grown plants, but in NO3 (-) grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 (-) grown plants, and lower activities of both, acidic and basic isoforms in NH4 (+)/NO3 (-)grown plants.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources
EP  - 534
IS  - 3
SP  - 530
VL  - 54
DO  - 10.1007/s10535-010-0093-0
ER  - 

@article{
author = "Vuletic, Mirjana M and Hadzi-Tasković Sukalović, Vesna and Marković, Ksenija and Dragišić Maksimović, Jelena",
year = "2010",
abstract = "The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 (-)) or both nitrogen forms, nitrate and ammonium (NH4 (+)/NO3 (-)). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 (+)/NO3 (-) in comparison with NO3 (-) grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 (+)/NO3 (-) treatment. The PEG treatment decreased enzyme activities in NH4 (+)/NO3 (-) grown plants, but in NO3 (-) grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 (-) grown plants, and lower activities of both, acidic and basic isoforms in NH4 (+)/NO3 (-)grown plants.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources",
pages = "534-530",
number = "3",
volume = "54",
doi = "10.1007/s10535-010-0093-0"
}

Vuletic, M. M., Hadzi-Tasković Sukalović, V., Marković, K.,& Dragišić Maksimović, J.. (2010). Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 54(3), 530-534.
https://doi.org/10.1007/s10535-010-0093-0

Vuletic MM, Hadzi-Tasković Sukalović V, Marković K, Dragišić Maksimović J. Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources. in Biologia Plantarum. 2010;54(3):530-534.
doi:10.1007/s10535-010-0093-0 .

Vuletic, Mirjana M, Hadzi-Tasković Sukalović, Vesna, Marković, Ksenija, Dragišić Maksimović, Jelena, "Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources" in Biologia Plantarum, 54, no. 3 (2010):530-534,
https://doi.org/10.1007/s10535-010-0093-0 . .

TY  - JOUR
AU  - Živković, Suzana
AU  - Popović, M
AU  - Dragišić Maksimović, Jelena
AU  - Momcilović, Ivana
AU  - Grubisic, Dragoljub V
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/377
AB  - Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated stale. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isofoms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.
EP  - 1081
IS  - 4
SP  - 1071
VL  - 62
DO  - 10.2298/ABS1004071Z
ER  - 

@article{
author = "Živković, Suzana and Popović, M and Dragišić Maksimović, Jelena and Momcilović, Ivana and Grubisic, Dragoljub V",
year = "2010",
abstract = "Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated stale. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isofoms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.",
pages = "1081-1071",
number = "4",
volume = "62",
doi = "10.2298/ABS1004071Z"
}

Živković, S., Popović, M., Dragišić Maksimović, J., Momcilović, I.,& Grubisic, D. V.. (2010). Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(4), 1071-1081.
https://doi.org/10.2298/ABS1004071Z

Živković S, Popović M, Dragišić Maksimović J, Momcilović I, Grubisic DV. Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.. in Archives of Biological Sciences. 2010;62(4):1071-1081.
doi:10.2298/ABS1004071Z .

Živković, Suzana, Popović, M, Dragišić Maksimović, Jelena, Momcilović, Ivana, Grubisic, Dragoljub V, "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l." in Archives of Biological Sciences, 62, no. 4 (2010):1071-1081,
https://doi.org/10.2298/ABS1004071Z . .

TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana M
AU  - Veljović-Jovanović, Sonja
AU  - Vučinić, Željko
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/373
AB  - Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles
EP  - 1557
IS  - 18
SP  - 1550
VL  - 167
DO  - 10.1016/j.jplph.2010.05.026
ER  - 

@article{
author = "Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana M and Veljović-Jovanović, Sonja and Vučinić, Željko",
year = "2010",
abstract = "Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles",
pages = "1557-1550",
number = "18",
volume = "167",
doi = "10.1016/j.jplph.2010.05.026"
}

Hadzi-Tasković Sukalović, V., Vuletic, M. M., Veljović-Jovanović, S.,& Vučinić, Ž.. (2010). The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 167(18), 1550-1557.
https://doi.org/10.1016/j.jplph.2010.05.026

Hadzi-Tasković Sukalović V, Vuletic MM, Veljović-Jovanović S, Vučinić Ž. The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology. 2010;167(18):1550-1557.
doi:10.1016/j.jplph.2010.05.026 .

Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana M, Veljović-Jovanović, Sonja, Vučinić, Željko, "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles" in Journal of Plant Physiology, 167, no. 18 (2010):1550-1557,
https://doi.org/10.1016/j.jplph.2010.05.026 . .

TY  - JOUR
AU  - Kukavica, Biljana
AU  - Mojović, Miloš
AU  - Vučinić, Željko
AU  - Maksimović, Vuk
AU  - Takahama, Umeo
AU  - Veljović-Jovanović, Sonja
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/368
AB  - The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation. For this mechanism, the apoplast or cell walls should contain the electron donors for (i) H2O2 formation from dioxygen; and (ii) the POD-catalyzed reduction of H2O2 to the hydroxyl radical. The aim of the work was to identify the electron donors in these reactions. In this report, hydroxyl radical (OH) generation in the cell wall isolated from pea roots was detected in the absence of any exogenous reductants, suggesting that the plant cell wall possesses the capacity to generate OH in situ. Distinct POD and Mn-superoxide dismutase (Mn-SOD) isoforms different from other cellular isoforms were shown by native gel electrophoresis to be preferably bound to the cell walls. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO), was used for detection of and differentiation between OH and the superoxide radical (O-2). The data obtained using POD inhibitors confirmed that tightly bound cell wall PODs are involved in DEPMPOOH adduct formation. A decrease in DEPMPOOH adduct formation in the presence of H2O2 scavengers demonstrated that this hydroxyl radical was derived from H2O2. During the generation of OH, the concentration of quinhydrone structures (as detected by EPR spectroscopy) increased, suggesting that the H2O2 required for the formation of OH in isolated cell walls is produced during the reduction of O-2 by hydroxycinnamic acids. Cell wall isolates in which the proteins have been denaturated (including the endogenous POD and SOD) did not produce OH. Addition of exogenous H2O2 again induced the production of OH, and these were shown to originate from the Fenton reaction with tightly bound metal ions. However, the appearance of the DEPMPOOOH adduct could also be observed, due to the production of O-2 when endogenous SOD has been inactivated. Also, O-2 was converted to OH in an in vitro horseradish peroxidase (HRP)H2O2 system to which exogenous SOD has been added. Taken together with the discovery of the cell wall-bound Mn-SOD isoform, these results support the role of such a cell wall-bound SOD in the formation of OH jointly with the cell wall-bound POD. According to the above findings, it seems that the hydroxycinnamic acids from the cell wall, acting as reductants, contribute to the formation of H2O2 in the presence of O-2 in an autocatalytic manner, and that POD and Mn-SOD coupled together generate OH from such H2O2.
PB  - Oxford Univ Press, Oxford
T2  - Plant and Cell Physiology
T1  - Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production
EP  - 317
IS  - 2
SP  - 304
VL  - 50
DO  - 10.1093/pcp/pcn199
ER  - 

@article{
author = "Kukavica, Biljana and Mojović, Miloš and Vučinić, Željko and Maksimović, Vuk and Takahama, Umeo and Veljović-Jovanović, Sonja",
year = "2009",
abstract = "The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation. For this mechanism, the apoplast or cell walls should contain the electron donors for (i) H2O2 formation from dioxygen; and (ii) the POD-catalyzed reduction of H2O2 to the hydroxyl radical. The aim of the work was to identify the electron donors in these reactions. In this report, hydroxyl radical (OH) generation in the cell wall isolated from pea roots was detected in the absence of any exogenous reductants, suggesting that the plant cell wall possesses the capacity to generate OH in situ. Distinct POD and Mn-superoxide dismutase (Mn-SOD) isoforms different from other cellular isoforms were shown by native gel electrophoresis to be preferably bound to the cell walls. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO), was used for detection of and differentiation between OH and the superoxide radical (O-2). The data obtained using POD inhibitors confirmed that tightly bound cell wall PODs are involved in DEPMPOOH adduct formation. A decrease in DEPMPOOH adduct formation in the presence of H2O2 scavengers demonstrated that this hydroxyl radical was derived from H2O2. During the generation of OH, the concentration of quinhydrone structures (as detected by EPR spectroscopy) increased, suggesting that the H2O2 required for the formation of OH in isolated cell walls is produced during the reduction of O-2 by hydroxycinnamic acids. Cell wall isolates in which the proteins have been denaturated (including the endogenous POD and SOD) did not produce OH. Addition of exogenous H2O2 again induced the production of OH, and these were shown to originate from the Fenton reaction with tightly bound metal ions. However, the appearance of the DEPMPOOOH adduct could also be observed, due to the production of O-2 when endogenous SOD has been inactivated. Also, O-2 was converted to OH in an in vitro horseradish peroxidase (HRP)H2O2 system to which exogenous SOD has been added. Taken together with the discovery of the cell wall-bound Mn-SOD isoform, these results support the role of such a cell wall-bound SOD in the formation of OH jointly with the cell wall-bound POD. According to the above findings, it seems that the hydroxycinnamic acids from the cell wall, acting as reductants, contribute to the formation of H2O2 in the presence of O-2 in an autocatalytic manner, and that POD and Mn-SOD coupled together generate OH from such H2O2.",
publisher = "Oxford Univ Press, Oxford",
journal = "Plant and Cell Physiology",
title = "Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production",
pages = "317-304",
number = "2",
volume = "50",
doi = "10.1093/pcp/pcn199"
}

Kukavica, B., Mojović, M., Vučinić, Ž., Maksimović, V., Takahama, U.,& Veljović-Jovanović, S.. (2009). Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production. in Plant and Cell Physiology
Oxford Univ Press, Oxford., 50(2), 304-317.
https://doi.org/10.1093/pcp/pcn199

Kukavica B, Mojović M, Vučinić Ž, Maksimović V, Takahama U, Veljović-Jovanović S. Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production. in Plant and Cell Physiology. 2009;50(2):304-317.
doi:10.1093/pcp/pcn199 .

Kukavica, Biljana, Mojović, Miloš, Vučinić, Željko, Maksimović, Vuk, Takahama, Umeo, Veljović-Jovanović, Sonja, "Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production" in Plant and Cell Physiology, 50, no. 2 (2009):304-317,
https://doi.org/10.1093/pcp/pcn199 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Živanović, Branka  D.
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/266
AB  - Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.
PB  - Elsevier Ireland Ltd, Clare
T2  - Plant Science
T1  - Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth
EP  - 662
IS  - 5
SP  - 656
VL  - 175
DO  - 10.1016/j.plantsci.2008.06.015
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Maksimović, Vuk and Živanović, Branka  D. and Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana",
year = "2008",
abstract = "Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Plant Science",
title = "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth",
pages = "662-656",
number = "5",
volume = "175",
doi = "10.1016/j.plantsci.2008.06.015"
}

Dragišić Maksimović, J., Maksimović, V., Živanović, Branka  D., Hadzi-Tasković Sukalović, V.,& Vuletic, M.. (2008). Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science
Elsevier Ireland Ltd, Clare., 175(5), 656-662.
https://doi.org/10.1016/j.plantsci.2008.06.015

Dragišić Maksimović J, Maksimović V, Živanović, Branka  D., Hadzi-Tasković Sukalović V, Vuletic M. Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science. 2008;175(5):656-662.
doi:10.1016/j.plantsci.2008.06.015 .

Dragišić Maksimović, Jelena, Maksimović, Vuk, Živanović, Branka  D., Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana, "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth" in Plant Science, 175, no. 5 (2008):656-662,
https://doi.org/10.1016/j.plantsci.2008.06.015 . .

TY  - JOUR
AU  - Todorović, Sladana
AU  - Giba, Zlatko
AU  - Bačić, Goran
AU  - Nikolic, Miroslav
AU  - Grubisic, Dragoljub
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/299
AB  - The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds
EP  - 324
IS  - 3
SP  - 322
VL  - 64
DO  - 10.1016/j.envexpbot.2008.07.001
ER  - 

@article{
author = "Todorović, Sladana and Giba, Zlatko and Bačić, Goran and Nikolic, Miroslav and Grubisic, Dragoljub",
year = "2008",
abstract = "The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds",
pages = "324-322",
number = "3",
volume = "64",
doi = "10.1016/j.envexpbot.2008.07.001"
}

Todorović, S., Giba, Z., Bačić, G., Nikolic, M.,& Grubisic, D.. (2008). High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 64(3), 322-324.
https://doi.org/10.1016/j.envexpbot.2008.07.001

Todorović S, Giba Z, Bačić G, Nikolic M, Grubisic D. High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany. 2008;64(3):322-324.
doi:10.1016/j.envexpbot.2008.07.001 .

Todorović, Sladana, Giba, Zlatko, Bačić, Goran, Nikolic, Miroslav, Grubisic, Dragoljub, "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds" in Environmental and Experimental Botany, 64, no. 3 (2008):322-324,
https://doi.org/10.1016/j.envexpbot.2008.07.001 . .