TY  - JOUR
AU  - Ćurčić, N.
AU  - Velićanski, A.
AU  - Cvetković, D.
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
AU  - Panković, Dejana M
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/625
AB  - Quinhydrone (QH) is a redox active charge transfer complex commonly used as a redox standard. Information on quinhydrone generation in plants is scarce and its physiological role is still unclear. Recently we have showed that excess zinc may induce oxidative stress through QH accumulation in the cell wall and stabilization of phenoxyl radicals [1]. The aim of our research was to investigate the antifungal activity of quinhydrone against yeast Saccharomyces cerevisiae (112, Hefebank Weihenstephan). Saccharomyces cerevisiae was grown on the Sabouraud maltose broth (HiMedia, Mumbai, India) in the presence of different concentrations of QH ranging from 75 mu M to 500 mu M. Concentrations of QH greater than 300 mu M had complete inhibitory effect on yeast growth, while lower concentrations (up to 200 mu M) did not affect the growth. QH had a significant impact on antioxidative defense enzymatic systems, indicated by the changes in the activity in catalase (CAT) and superoxide dismutase (SOD). CAT activities increased by 43% (150 mu M QH) and SOD activities by 122% and 60% at 150 mu M and 220 mu M QH respectively. However, abrupt inhibition of both enzymes was observed at concentrations higher than 220 mu M QH (>= 70%), to be almost completely diminished at 280 mu M QH. High molecular weight genomic DNA without any laddering or smearing was detected in both control and QH treated yeast cells, indicating the absence of apoptosis.
T2  - Fresenius Environmental Bulletin
T1  - Antifungal activity of quinhydrone against saccharomyces cerevisiae
EP  - 1762
IS  - 6
SP  - 1758
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_625
ER  - 

@article{
author = "Ćurčić, N. and Velićanski, A. and Cvetković, D. and Morina, Filis and Veljović-Jovanović, Sonja and Panković, Dejana M",
year = "2013",
abstract = "Quinhydrone (QH) is a redox active charge transfer complex commonly used as a redox standard. Information on quinhydrone generation in plants is scarce and its physiological role is still unclear. Recently we have showed that excess zinc may induce oxidative stress through QH accumulation in the cell wall and stabilization of phenoxyl radicals [1]. The aim of our research was to investigate the antifungal activity of quinhydrone against yeast Saccharomyces cerevisiae (112, Hefebank Weihenstephan). Saccharomyces cerevisiae was grown on the Sabouraud maltose broth (HiMedia, Mumbai, India) in the presence of different concentrations of QH ranging from 75 mu M to 500 mu M. Concentrations of QH greater than 300 mu M had complete inhibitory effect on yeast growth, while lower concentrations (up to 200 mu M) did not affect the growth. QH had a significant impact on antioxidative defense enzymatic systems, indicated by the changes in the activity in catalase (CAT) and superoxide dismutase (SOD). CAT activities increased by 43% (150 mu M QH) and SOD activities by 122% and 60% at 150 mu M and 220 mu M QH respectively. However, abrupt inhibition of both enzymes was observed at concentrations higher than 220 mu M QH (>= 70%), to be almost completely diminished at 280 mu M QH. High molecular weight genomic DNA without any laddering or smearing was detected in both control and QH treated yeast cells, indicating the absence of apoptosis.",
journal = "Fresenius Environmental Bulletin",
title = "Antifungal activity of quinhydrone against saccharomyces cerevisiae",
pages = "1762-1758",
number = "6",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_625"
}

Ćurčić, N., Velićanski, A., Cvetković, D., Morina, F., Veljović-Jovanović, S.,& Panković, D. M.. (2013). Antifungal activity of quinhydrone against saccharomyces cerevisiae. in Fresenius Environmental Bulletin, 22(6), 1758-1762.
https://hdl.handle.net/21.15107/rcub_rimsi_625

Ćurčić N, Velićanski A, Cvetković D, Morina F, Veljović-Jovanović S, Panković DM. Antifungal activity of quinhydrone against saccharomyces cerevisiae. in Fresenius Environmental Bulletin. 2013;22(6):1758-1762.
https://hdl.handle.net/21.15107/rcub_rimsi_625 .

Ćurčić, N., Velićanski, A., Cvetković, D., Morina, Filis, Veljović-Jovanović, Sonja, Panković, Dejana M, "Antifungal activity of quinhydrone against saccharomyces cerevisiae" in Fresenius Environmental Bulletin, 22, no. 6 (2013):1758-1762,
https://hdl.handle.net/21.15107/rcub_rimsi_625 .

TY  - JOUR
AU  - Morina, Filis
AU  - Jovanović, Ljubinko
AU  - Mojović, Miloš
AU  - Vidović, Marija
AU  - Panković, Dejana M
AU  - Veljović-Jovanović, Sonja
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/379
AB  - Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn2+. Reactive oxygen species (ROS) accumulation was demonstrated by the fluorescent probe H(2)DCFDA and EPR measurements. The extent of zinc-induced oxidative damage was assessed by measuring the level of protein carbonylation. Activities and isoform profiles of some antioxidant enzymes and the changes in ascorbate and total phenolic contents of leaves and roots were determined. Stunted growth because of zinc accumulation, preferentially in the roots, was accompanied by H2O2 production in the leaf and root apoplasts. Increased EPR signals of the endogenous oxidant quinhydrone, center dot CH3 and center dot OH, were found in the cell walls of zinc-treated plants. The activities of the antioxidative enzymes ascorbate peroxidase (APX) (EC 1.11.1.11), soluble superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD), (EC 1.11.1.7) and monodehydroascorbate reductase (EC 1.6.5.4) were increased; those of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and ascorbate oxidase (AAO) (EC 1.10.3.3) were decreased with zinc treatment. Zinc induced a cell-wall-bound SOD isoform in both organs. Leaves accumulated more ascorbate and phenolics in comparison to roots. We propose a mechanism for zinc-promoted oxidative stress in V. thapsus L. through the generation of charge transfer complexes and quinhydrone because of phenoxyl radical stabilisation by Zn2+ in the cell wall. Our results suggest that the SOD and APX responses are mediated by ROS accumulation in the apoplast. The importance of the POD/Phe/AA (ascorbic acid) scavenging system in the apoplast is also discussed.
PB  - Wiley, Hoboken
T2  - Physiologia Plantarum
T1  - Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall
EP  - 224
IS  - 3
SP  - 209
VL  - 140
DO  - 10.1111/j.1399-3054.2010.01399.x
ER  - 

@article{
author = "Morina, Filis and Jovanović, Ljubinko and Mojović, Miloš and Vidović, Marija and Panković, Dejana M and Veljović-Jovanović, Sonja",
year = "2010",
abstract = "Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn2+. Reactive oxygen species (ROS) accumulation was demonstrated by the fluorescent probe H(2)DCFDA and EPR measurements. The extent of zinc-induced oxidative damage was assessed by measuring the level of protein carbonylation. Activities and isoform profiles of some antioxidant enzymes and the changes in ascorbate and total phenolic contents of leaves and roots were determined. Stunted growth because of zinc accumulation, preferentially in the roots, was accompanied by H2O2 production in the leaf and root apoplasts. Increased EPR signals of the endogenous oxidant quinhydrone, center dot CH3 and center dot OH, were found in the cell walls of zinc-treated plants. The activities of the antioxidative enzymes ascorbate peroxidase (APX) (EC 1.11.1.11), soluble superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD), (EC 1.11.1.7) and monodehydroascorbate reductase (EC 1.6.5.4) were increased; those of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and ascorbate oxidase (AAO) (EC 1.10.3.3) were decreased with zinc treatment. Zinc induced a cell-wall-bound SOD isoform in both organs. Leaves accumulated more ascorbate and phenolics in comparison to roots. We propose a mechanism for zinc-promoted oxidative stress in V. thapsus L. through the generation of charge transfer complexes and quinhydrone because of phenoxyl radical stabilisation by Zn2+ in the cell wall. Our results suggest that the SOD and APX responses are mediated by ROS accumulation in the apoplast. The importance of the POD/Phe/AA (ascorbic acid) scavenging system in the apoplast is also discussed.",
publisher = "Wiley, Hoboken",
journal = "Physiologia Plantarum",
title = "Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall",
pages = "224-209",
number = "3",
volume = "140",
doi = "10.1111/j.1399-3054.2010.01399.x"
}

Morina, F., Jovanović, L., Mojović, M., Vidović, M., Panković, D. M.,& Veljović-Jovanović, S.. (2010). Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall. in Physiologia Plantarum
Wiley, Hoboken., 140(3), 209-224.
https://doi.org/10.1111/j.1399-3054.2010.01399.x

Morina F, Jovanović L, Mojović M, Vidović M, Panković DM, Veljović-Jovanović S. Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall. in Physiologia Plantarum. 2010;140(3):209-224.
doi:10.1111/j.1399-3054.2010.01399.x .

Morina, Filis, Jovanović, Ljubinko, Mojović, Miloš, Vidović, Marija, Panković, Dejana M, Veljović-Jovanović, Sonja, "Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall" in Physiologia Plantarum, 140, no. 3 (2010):209-224,
https://doi.org/10.1111/j.1399-3054.2010.01399.x . .