TY  - JOUR
AU  - Karaman, Maja
AU  - Tesanović, Kristina
AU  - Gorjanović, Stanislava
AU  - Pastor, Ferenc
AU  - Simonović, Mladen
AU  - Glumac, Miodrag
AU  - Pejin, Boris
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1510
AB  - This study was focused on in vitro screening of the total antioxidant activity of the selected extracts of the mushroom Coprinus comatus and quinic acid, one of their antidiabetic ingredients, by an uncommon electrochemical assay. Indeed, direct current (DC) polarographic HydroxoPerhydroxo Mercury(II) Complex (HPMC) assay based on decrease of anodic limiting current originating from HPMC formation in alkaline solutions of hydrogen peroxide at potential of mercury dissolution, observed upon gradual addition of antioxidants, was applied herein for the estimation of the natural products' antioxidativity. Quinic acid was found to exhibit most promising antioxidant potential (4.0 +/- 0.2%mu L-1) being approximate to 2-fold more active than the screened C. comatus extract samples. Actually, such a finding puts some light on the antioxidativity of cyclic polyols, well understimated class of organic compounds, compared to aromatic (poly)phenolics. As a low cost, easy-to handle and accurate this polarographic assay may be thoroughly recommended for much broader use.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Polarography as a technique of choice for the evaluation of total antioxidant activity: The case study of selected Coprinus Comatus extracts and quinic acid, their antidiabetic ingredient
EP  - 1716
IS  - 10
SP  - 1711
VL  - 35
DO  - 10.1080/14786419.2019.1628753
ER  - 

@article{
author = "Karaman, Maja and Tesanović, Kristina and Gorjanović, Stanislava and Pastor, Ferenc and Simonović, Mladen and Glumac, Miodrag and Pejin, Boris",
year = "2021",
abstract = "This study was focused on in vitro screening of the total antioxidant activity of the selected extracts of the mushroom Coprinus comatus and quinic acid, one of their antidiabetic ingredients, by an uncommon electrochemical assay. Indeed, direct current (DC) polarographic HydroxoPerhydroxo Mercury(II) Complex (HPMC) assay based on decrease of anodic limiting current originating from HPMC formation in alkaline solutions of hydrogen peroxide at potential of mercury dissolution, observed upon gradual addition of antioxidants, was applied herein for the estimation of the natural products' antioxidativity. Quinic acid was found to exhibit most promising antioxidant potential (4.0 +/- 0.2%mu L-1) being approximate to 2-fold more active than the screened C. comatus extract samples. Actually, such a finding puts some light on the antioxidativity of cyclic polyols, well understimated class of organic compounds, compared to aromatic (poly)phenolics. As a low cost, easy-to handle and accurate this polarographic assay may be thoroughly recommended for much broader use.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Polarography as a technique of choice for the evaluation of total antioxidant activity: The case study of selected Coprinus Comatus extracts and quinic acid, their antidiabetic ingredient",
pages = "1716-1711",
number = "10",
volume = "35",
doi = "10.1080/14786419.2019.1628753"
}

Karaman, M., Tesanović, K., Gorjanović, S., Pastor, F., Simonović, M., Glumac, M.,& Pejin, B.. (2021). Polarography as a technique of choice for the evaluation of total antioxidant activity: The case study of selected Coprinus Comatus extracts and quinic acid, their antidiabetic ingredient. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 35(10), 1711-1716.
https://doi.org/10.1080/14786419.2019.1628753

Karaman M, Tesanović K, Gorjanović S, Pastor F, Simonović M, Glumac M, Pejin B. Polarography as a technique of choice for the evaluation of total antioxidant activity: The case study of selected Coprinus Comatus extracts and quinic acid, their antidiabetic ingredient. in Natural Product Research. 2021;35(10):1711-1716.
doi:10.1080/14786419.2019.1628753 .

Karaman, Maja, Tesanović, Kristina, Gorjanović, Stanislava, Pastor, Ferenc, Simonović, Mladen, Glumac, Miodrag, Pejin, Boris, "Polarography as a technique of choice for the evaluation of total antioxidant activity: The case study of selected Coprinus Comatus extracts and quinic acid, their antidiabetic ingredient" in Natural Product Research, 35, no. 10 (2021):1711-1716,
https://doi.org/10.1080/14786419.2019.1628753 . .

TY  - JOUR
AU  - Zakrzewska, Joanna
AU  - Mitrović, Aleksandra Lj.
AU  - Mutavdžić, Dragosav
AU  - Ducic, Tanja
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1394
AB  - The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence
EP  - 3328
IS  - 3
SP  - 3320
VL  - 27
DO  - 10.1007/s11356-019-07200-9
ER  - 

@article{
author = "Zakrzewska, Joanna and Mitrović, Aleksandra Lj. and Mutavdžić, Dragosav and Ducic, Tanja and Radotić, Ksenija",
year = "2020",
abstract = "The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence",
pages = "3328-3320",
number = "3",
volume = "27",
doi = "10.1007/s11356-019-07200-9"
}

Zakrzewska, J., Mitrović, A. Lj., Mutavdžić, D., Ducic, T.,& Radotić, K.. (2020). Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(3), 3320-3328.
https://doi.org/10.1007/s11356-019-07200-9

Zakrzewska J, Mitrović AL, Mutavdžić D, Ducic T, Radotić K. Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research. 2020;27(3):3320-3328.
doi:10.1007/s11356-019-07200-9 .

Zakrzewska, Joanna, Mitrović, Aleksandra Lj., Mutavdžić, Dragosav, Ducic, Tanja, Radotić, Ksenija, "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence" in Environmental Science and Pollution Research, 27, no. 3 (2020):3320-3328,
https://doi.org/10.1007/s11356-019-07200-9 . .

TY  - JOUR
AU  - Cvetić-Antić, Tijana
AU  - Janosević, Dusica
AU  - Maksimović, Vuk
AU  - Zivić, Miroslav
AU  - Budimir, Snežana M
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra Lj.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1383
AB  - We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
VL  - 244
DO  - 10.1016/j.jplph.2019.153086
ER  - 

@article{
author = "Cvetić-Antić, Tijana and Janosević, Dusica and Maksimović, Vuk and Zivić, Miroslav and Budimir, Snežana M and Glamočlija, Jasmina and Mitrović, Aleksandra Lj.",
year = "2020",
abstract = "We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro",
volume = "244",
doi = "10.1016/j.jplph.2019.153086"
}

Cvetić-Antić, T., Janosević, D., Maksimović, V., Zivić, M., Budimir, S. M., Glamočlija, J.,& Mitrović, A. Lj.. (2020). Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 244.
https://doi.org/10.1016/j.jplph.2019.153086

Cvetić-Antić T, Janosević D, Maksimović V, Zivić M, Budimir SM, Glamočlija J, Mitrović AL. Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology. 2020;244.
doi:10.1016/j.jplph.2019.153086 .

Cvetić-Antić, Tijana, Janosević, Dusica, Maksimović, Vuk, Zivić, Miroslav, Budimir, Snežana M, Glamočlija, Jasmina, Mitrović, Aleksandra Lj., "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro" in Journal of Plant Physiology, 244 (2020),
https://doi.org/10.1016/j.jplph.2019.153086 . .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Maksimović, Vuk
AU  - Dragišić Maksimović, Jelena
AU  - Stanković, Mira
AU  - Krstović, Sasa
AU  - Baosic, Rada
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1358
AB  - BACKGROUND Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose-response manner, with a ratio of below 1 up to a concentration of 51.51 mu g kg(-1), and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 mu g kg(-1), respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose-response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship
EP  - 2910
IS  - 7
SP  - 2905
VL  - 100
DO  - 10.1002/jsfa.10317
ER  - 

@article{
author = "Bartolić, Dragana and Maksimović, Vuk and Dragišić Maksimović, Jelena and Stanković, Mira and Krstović, Sasa and Baosic, Rada and Radotić, Ksenija",
year = "2020",
abstract = "BACKGROUND Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose-response manner, with a ratio of below 1 up to a concentration of 51.51 mu g kg(-1), and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 mu g kg(-1), respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose-response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship",
pages = "2910-2905",
number = "7",
volume = "100",
doi = "10.1002/jsfa.10317"
}

Bartolić, D., Maksimović, V., Dragišić Maksimović, J., Stanković, M., Krstović, S., Baosic, R.,& Radotić, K.. (2020). Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 100(7), 2905-2910.
https://doi.org/10.1002/jsfa.10317

Bartolić D, Maksimović V, Dragišić Maksimović J, Stanković M, Krstović S, Baosic R, Radotić K. Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship. in Journal of the Science of Food and Agriculture. 2020;100(7):2905-2910.
doi:10.1002/jsfa.10317 .

Bartolić, Dragana, Maksimović, Vuk, Dragišić Maksimović, Jelena, Stanković, Mira, Krstović, Sasa, Baosic, Rada, Radotić, Ksenija, "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship" in Journal of the Science of Food and Agriculture, 100, no. 7 (2020):2905-2910,
https://doi.org/10.1002/jsfa.10317 . .

TY  - JOUR
AU  - Milosavljević, Dragica
AU  - Mutavdžić, Dragosav
AU  - Radotić, Ksenija
AU  - Milivojević, Jasminka M
AU  - Maksimović, Vuk
AU  - Dragišić Maksimović, Jelena
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1359
AB  - Phenolic compounds of 12 strawberry cultivars were profiled using spectrophotometry, spectrofluorometry, and high-performance liquid chromatography-mass spectrometry, coupled with multivariate regression analysis. Total phenolic content, total anthocyanin content, and total antioxidant capacity (TPC, TACY, and TAC, respectively) and concentrations of individual phenolics were evaluated, and the multivariate statistic was employed to identify the most promising cultivars based on their phenolic content. According to the principal component analysis, TAC was strongly correlated with the TPC (0.81), pointing out its importance in overall antioxidant activity. 'Joly', 'Laetitia', and 'Asia' cultivars were distinguished from others as the richest in concerning identified anthocyanins, almost all flavonoids and phenolic acids. Multivariate curve resolution with alternating least squares indicated the presence of two main types of fluorophores assigned to anthocyanins and phenolics, in which emission spectral ratios also showed the highest values in the referred cultivars. These cultivars could be recommended to consumers in terms of the health functionality of fruit.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods
EP  - 4354
IS  - 15
SP  - 4346
VL  - 68
DO  - 10.1021/acs.jafc.9b07331
ER  - 

@article{
author = "Milosavljević, Dragica and Mutavdžić, Dragosav and Radotić, Ksenija and Milivojević, Jasminka M and Maksimović, Vuk and Dragišić Maksimović, Jelena",
year = "2020",
abstract = "Phenolic compounds of 12 strawberry cultivars were profiled using spectrophotometry, spectrofluorometry, and high-performance liquid chromatography-mass spectrometry, coupled with multivariate regression analysis. Total phenolic content, total anthocyanin content, and total antioxidant capacity (TPC, TACY, and TAC, respectively) and concentrations of individual phenolics were evaluated, and the multivariate statistic was employed to identify the most promising cultivars based on their phenolic content. According to the principal component analysis, TAC was strongly correlated with the TPC (0.81), pointing out its importance in overall antioxidant activity. 'Joly', 'Laetitia', and 'Asia' cultivars were distinguished from others as the richest in concerning identified anthocyanins, almost all flavonoids and phenolic acids. Multivariate curve resolution with alternating least squares indicated the presence of two main types of fluorophores assigned to anthocyanins and phenolics, in which emission spectral ratios also showed the highest values in the referred cultivars. These cultivars could be recommended to consumers in terms of the health functionality of fruit.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods",
pages = "4354-4346",
number = "15",
volume = "68",
doi = "10.1021/acs.jafc.9b07331"
}

Milosavljević, D., Mutavdžić, D., Radotić, K., Milivojević, J. M., Maksimović, V.,& Dragišić Maksimović, J.. (2020). Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 68(15), 4346-4354.
https://doi.org/10.1021/acs.jafc.9b07331

Milosavljević D, Mutavdžić D, Radotić K, Milivojević JM, Maksimović V, Dragišić Maksimović J. Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods. in Journal of Agricultural and Food Chemistry. 2020;68(15):4346-4354.
doi:10.1021/acs.jafc.9b07331 .

Milosavljević, Dragica, Mutavdžić, Dragosav, Radotić, Ksenija, Milivojević, Jasminka M, Maksimović, Vuk, Dragišić Maksimović, Jelena, "Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods" in Journal of Agricultural and Food Chemistry, 68, no. 15 (2020):4346-4354,
https://doi.org/10.1021/acs.jafc.9b07331 . .

TY  - THES
AU  - Dimitrijević, Milena
PY  - 2020
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7470
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22312/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=17198857
UR  - https://nardus.mpn.gov.rs/handle/123456789/17289
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/26
AB  - Biliverdin (BV), proizvod katabolizma hem grupe, u interakciji sa prelaznim metalima može da gradi komplekse, ali detalji ovih interakcija u fiziološkim uslovima nisu dovoljno opisani. Ovde su ispitane koordicione/redoks interakcije BV sa jonima Cu(II) u fosfatnom puferu na pH 7,4, koristeći ultraljubičastu-vidljivu spektrofotometriju, masenu spektrometriju, ramansku spektroskopija, metodu nuklearne magnetne rezonacije (NMR), metodu elektronske paramagnetne rezonacije (EPR), fluorimetrijske i elektrohemijske metode. Pokazano je da BV formira stabilan koordinacioni kompleks sa jonima bakra u stehiometriji 1:1. Struktura BV je više planarna i energijski stabilna u kompleksu. Kompleks je pokazao jake paramagnetne efekte koji su pripisani nesparenom delokalizovanom elektronu. Delokalizovani elektron može poticati iz BV ili od jona Cu(II), pa je kompleks sastavljen ili od katjona BV radikala i jona Cu(I) ili od anjona BV radikala i jona Cu(III). Kompleks je podložan oksidaciji samo u prisustvu O2 i višku jona Cu(II), ili jakog oksidacionog sredstva, dok je otporan na redukciona sredstva. Biološki efekti stabilnog metalokompleksa BV koji sadrže delokalizovani nesparni elektron zahtevaju dalje istraživanje. Ovo može dati odgovor na dugogodišnje pitanje razloga ulaganje velike količine energije u katabolizam BV, koji sam po sebi predstavlja relativno bezopasan molekul...
AB  - Biliverdin (BV) is one of the products of heme catabolism that is known to interact with redox-active metals. (Patho)physiological roles of BV and details of its interactions with metals under physiological conditions, which may be interconnected, are not fully understood. In the current work, we examined coordinate and redox interactions of BV with copper ions (Cu(II)). The interactions were exaimined under conditions that mimic physiological settings - in the phosphate buffer with pH 7.4. A battery of physico-chemical methods were applied, including UV-VIS spectrophotometry, fluorescence spectroscopy, nuclear magnetic resonance spectroscopy (NMR), mass spectrometry, Raman spectroscopy, electron paramagnetic resonance (EPR), and electrochemical methods. BV and copper formed a stable coordination complex with 1 : 1 stoichiometry, with BV showing a more planar structure and energetic stability in the complex. The complex showed strong paramagnetic effects, most likely due to a delocalized unpaired electron. The delocalized e- may come from copper ion or BV.  The complex is formally composed of BV radical cation and Cu(I) ion or of BV radical anion and Cu(III) ion. The complex underwent oxidation only in the presence of both O2 and an excess of Cu(II) ion, or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se...
PB  - Univerzitet u Beogradu, Fakultet za fizičku hemiju
T1  - Spektroskopska i redoks analiza kompleksa biliverdina sa jonima bakra (II)
T1  - Spectroscopis and redox analysis of complex of biliverdin with copper (II) ions
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17289
ER  - 

@phdthesis{
author = "Dimitrijević, Milena",
year = "2020",
abstract = "Biliverdin (BV), proizvod katabolizma hem grupe, u interakciji sa prelaznim metalima može da gradi komplekse, ali detalji ovih interakcija u fiziološkim uslovima nisu dovoljno opisani. Ovde su ispitane koordicione/redoks interakcije BV sa jonima Cu(II) u fosfatnom puferu na pH 7,4, koristeći ultraljubičastu-vidljivu spektrofotometriju, masenu spektrometriju, ramansku spektroskopija, metodu nuklearne magnetne rezonacije (NMR), metodu elektronske paramagnetne rezonacije (EPR), fluorimetrijske i elektrohemijske metode. Pokazano je da BV formira stabilan koordinacioni kompleks sa jonima bakra u stehiometriji 1:1. Struktura BV je više planarna i energijski stabilna u kompleksu. Kompleks je pokazao jake paramagnetne efekte koji su pripisani nesparenom delokalizovanom elektronu. Delokalizovani elektron može poticati iz BV ili od jona Cu(II), pa je kompleks sastavljen ili od katjona BV radikala i jona Cu(I) ili od anjona BV radikala i jona Cu(III). Kompleks je podložan oksidaciji samo u prisustvu O2 i višku jona Cu(II), ili jakog oksidacionog sredstva, dok je otporan na redukciona sredstva. Biološki efekti stabilnog metalokompleksa BV koji sadrže delokalizovani nesparni elektron zahtevaju dalje istraživanje. Ovo može dati odgovor na dugogodišnje pitanje razloga ulaganje velike količine energije u katabolizam BV, koji sam po sebi predstavlja relativno bezopasan molekul..., Biliverdin (BV) is one of the products of heme catabolism that is known to interact with redox-active metals. (Patho)physiological roles of BV and details of its interactions with metals under physiological conditions, which may be interconnected, are not fully understood. In the current work, we examined coordinate and redox interactions of BV with copper ions (Cu(II)). The interactions were exaimined under conditions that mimic physiological settings - in the phosphate buffer with pH 7.4. A battery of physico-chemical methods were applied, including UV-VIS spectrophotometry, fluorescence spectroscopy, nuclear magnetic resonance spectroscopy (NMR), mass spectrometry, Raman spectroscopy, electron paramagnetic resonance (EPR), and electrochemical methods. BV and copper formed a stable coordination complex with 1 : 1 stoichiometry, with BV showing a more planar structure and energetic stability in the complex. The complex showed strong paramagnetic effects, most likely due to a delocalized unpaired electron. The delocalized e- may come from copper ion or BV.  The complex is formally composed of BV radical cation and Cu(I) ion or of BV radical anion and Cu(III) ion. The complex underwent oxidation only in the presence of both O2 and an excess of Cu(II) ion, or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se...",
publisher = "Univerzitet u Beogradu, Fakultet za fizičku hemiju",
title = "Spektroskopska i redoks analiza kompleksa biliverdina sa jonima bakra (II), Spectroscopis and redox analysis of complex of biliverdin with copper (II) ions",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17289"
}

Dimitrijević, M.. (2020). Spektroskopska i redoks analiza kompleksa biliverdina sa jonima bakra (II). 
Univerzitet u Beogradu, Fakultet za fizičku hemiju..
https://hdl.handle.net/21.15107/rcub_nardus_17289

Dimitrijević M. Spektroskopska i redoks analiza kompleksa biliverdina sa jonima bakra (II). 2020;.
https://hdl.handle.net/21.15107/rcub_nardus_17289 .

Dimitrijević, Milena, "Spektroskopska i redoks analiza kompleksa biliverdina sa jonima bakra (II)" (2020),
https://hdl.handle.net/21.15107/rcub_nardus_17289 .

TY  - JOUR
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Maksimović, Vuk
AU  - Ciric, Ana
AU  - Ivanov, Marija
AU  - Bozunović, Jelena
AU  - Banjanac, Tijana
AU  - Misic, Danijela
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1368
AB  - Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH center dot and ABTS(center dot+)), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(-)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.
PB  - Elsevier, Amsterdam
T2  - South African Journal of Botany
T1  - Antioxidant and antimicrobial activity of two Asplenium species
EP  - 187
SP  - 180
VL  - 132
DO  - 10.1016/j.sajb.2020.03.034
ER  - 

@article{
author = "Živković, Suzana and Milutinović, Milica and Maksimović, Vuk and Ciric, Ana and Ivanov, Marija and Bozunović, Jelena and Banjanac, Tijana and Misic, Danijela",
year = "2020",
abstract = "Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH center dot and ABTS(center dot+)), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(-)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.",
publisher = "Elsevier, Amsterdam",
journal = "South African Journal of Botany",
title = "Antioxidant and antimicrobial activity of two Asplenium species",
pages = "187-180",
volume = "132",
doi = "10.1016/j.sajb.2020.03.034"
}

Živković, S., Milutinović, M., Maksimović, V., Ciric, A., Ivanov, M., Bozunović, J., Banjanac, T.,& Misic, D.. (2020). Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany
Elsevier, Amsterdam., 132, 180-187.
https://doi.org/10.1016/j.sajb.2020.03.034

Živković S, Milutinović M, Maksimović V, Ciric A, Ivanov M, Bozunović J, Banjanac T, Misic D. Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany. 2020;132:180-187.
doi:10.1016/j.sajb.2020.03.034 .

Živković, Suzana, Milutinović, Milica, Maksimović, Vuk, Ciric, Ana, Ivanov, Marija, Bozunović, Jelena, Banjanac, Tijana, Misic, Danijela, "Antioxidant and antimicrobial activity of two Asplenium species" in South African Journal of Botany, 132 (2020):180-187,
https://doi.org/10.1016/j.sajb.2020.03.034 . .

TY  - JOUR
AU  - Karaman, Maja
AU  - Tesanović, Kristina
AU  - Novaković, Aleksandra
AU  - Jakovljević, Dragica
AU  - Janjusević, Ljiljana
AU  - Sibul, Filip
AU  - Pejin, Boris
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1318
AB  - In vitroacetylholinesterase (AChE) inhibitory activity of an autochthonous sample of the mushroomCoprinus comatus(encompassing fruiting body FB, mycelia M and filtrate F from the submerged cultivation) was the subject of this study.C. comatusF extract exhibited rather potent anti-AChE activity (73.0 +/- 1.5%) atin liquidconditions, comparable to those of the conventional drug donepezil (80.6 +/- 1.4%). Also, the same extract exhibited high anti-AChE activity (1 mu g)in solid. While its FTIR spectrum indicated the presence of phenolic compounds, quercetin (28.1 mu g g(-1)d.w.) was found to affect the observed bioactivity (59.8 +/- 0.9%). This is the first report of profound anti-AChE activity of anyC. comatusextract, a medicinal mushroom that has been successfully cultivated in P.R. China, due to the demanding needs of food industry.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Coprinus comatusfiltrate extract, a novel neuroprotective agent of natural origin
EP  - 2350
IS  - 16
SP  - 2346
VL  - 34
DO  - 10.1080/14786419.2018.1533831
ER  - 

@article{
author = "Karaman, Maja and Tesanović, Kristina and Novaković, Aleksandra and Jakovljević, Dragica and Janjusević, Ljiljana and Sibul, Filip and Pejin, Boris",
year = "2020",
abstract = "In vitroacetylholinesterase (AChE) inhibitory activity of an autochthonous sample of the mushroomCoprinus comatus(encompassing fruiting body FB, mycelia M and filtrate F from the submerged cultivation) was the subject of this study.C. comatusF extract exhibited rather potent anti-AChE activity (73.0 +/- 1.5%) atin liquidconditions, comparable to those of the conventional drug donepezil (80.6 +/- 1.4%). Also, the same extract exhibited high anti-AChE activity (1 mu g)in solid. While its FTIR spectrum indicated the presence of phenolic compounds, quercetin (28.1 mu g g(-1)d.w.) was found to affect the observed bioactivity (59.8 +/- 0.9%). This is the first report of profound anti-AChE activity of anyC. comatusextract, a medicinal mushroom that has been successfully cultivated in P.R. China, due to the demanding needs of food industry.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Coprinus comatusfiltrate extract, a novel neuroprotective agent of natural origin",
pages = "2350-2346",
number = "16",
volume = "34",
doi = "10.1080/14786419.2018.1533831"
}

Karaman, M., Tesanović, K., Novaković, A., Jakovljević, D., Janjusević, L., Sibul, F.,& Pejin, B.. (2020). Coprinus comatusfiltrate extract, a novel neuroprotective agent of natural origin. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 34(16), 2346-2350.
https://doi.org/10.1080/14786419.2018.1533831

Karaman M, Tesanović K, Novaković A, Jakovljević D, Janjusević L, Sibul F, Pejin B. Coprinus comatusfiltrate extract, a novel neuroprotective agent of natural origin. in Natural Product Research. 2020;34(16):2346-2350.
doi:10.1080/14786419.2018.1533831 .

Karaman, Maja, Tesanović, Kristina, Novaković, Aleksandra, Jakovljević, Dragica, Janjusević, Ljiljana, Sibul, Filip, Pejin, Boris, "Coprinus comatusfiltrate extract, a novel neuroprotective agent of natural origin" in Natural Product Research, 34, no. 16 (2020):2346-2350,
https://doi.org/10.1080/14786419.2018.1533831 . .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetic Antic, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2418
AB  - Vanadium enters mycelium of fungus P. blakesleeanus in both +5 and +4 oxidation states. Mycelium in three stages of growth (20h, 36h and 56h) was treated with three concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. The decrease in viability of mycelium was noticed only at 36h for all applied concentrations. Glutathione has a role in reduction of V+5 to a less toxic form V+4 , so we assayed the changes in total glutathione. Mycelium in early exponential phase of growth (20 h), after 1h or 5h treatment, showed concentration dependent decrease in glutathione, with the largest decrease of 40±4% after 1h and 22±9% after 5h treatment induced by 10 mM V+5. In stationary phase of growth (56h), changes in glutathione concentration were noticed only after 1h of treatment, and the largest decrease (36±6%) was induced by 1 mM V+5. Activities of glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx), have also been examined. Increase in the activities of GPx and GST was noticed in 56h old mycelia treated for 5h with 5 mM and 10 mM V+5, but the only statistically significant increase (54±20%) was noticed in GST activity after 5h of 10 mM V+5 treatment.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia
T1  - Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus
EP  - 118
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2418
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetic Antic, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2019",
abstract = "Vanadium enters mycelium of fungus P. blakesleeanus in both +5 and +4 oxidation states. Mycelium in three stages of growth (20h, 36h and 56h) was treated with three concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. The decrease in viability of mycelium was noticed only at 36h for all applied concentrations. Glutathione has a role in reduction of V+5 to a less toxic form V+4 , so we assayed the changes in total glutathione. Mycelium in early exponential phase of growth (20 h), after 1h or 5h treatment, showed concentration dependent decrease in glutathione, with the largest decrease of 40±4% after 1h and 22±9% after 5h treatment induced by 10 mM V+5. In stationary phase of growth (56h), changes in glutathione concentration were noticed only after 1h of treatment, and the largest decrease (36±6%) was induced by 1 mM V+5. Activities of glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx), have also been examined. Increase in the activities of GPx and GST was noticed in 56h old mycelia treated for 5h with 5 mM and 10 mM V+5, but the only statistically significant increase (54±20%) was noticed in GST activity after 5h of 10 mM V+5 treatment.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia",
title = "Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus",
pages = "118-118",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2418"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetic Antic, T., Živić, M.,& Stanić, M.. (2019). Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus. in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 118-118.
https://hdl.handle.net/21.15107/rcub_rimsi_2418

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetic Antic T, Živić M, Stanić M. Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus. in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia. 2019;:118-118.
https://hdl.handle.net/21.15107/rcub_rimsi_2418 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetic Antic, Tijana, Živić, Miroslav, Stanić, Marina, "Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus" in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia (2019):118-118,
https://hdl.handle.net/21.15107/rcub_rimsi_2418 .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Mitrović, Aleksandra Lj.
AU  - Algarra, Manuel
AU  - Lazaro-Martinez, Juan M.
AU  - Rodriguez-Castellon, Enrique
AU  - Maksimović, Vuk
AU  - Spasić, Slađana
AU  - Beskoski, Vladimir P.
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1203
AB  - Reports about the influence of cerium-oxide nanoparticles (nCeO(2)) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO(2) with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO(2) were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO(2) on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL(-1) of nCeO(2) on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO(2) was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO(2) had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development
IS  - 11
VL  - 8
DO  - 10.3390/plants8110478
ER  - 

@article{
author = "Milenković, Ivana and Mitrović, Aleksandra Lj. and Algarra, Manuel and Lazaro-Martinez, Juan M. and Rodriguez-Castellon, Enrique and Maksimović, Vuk and Spasić, Slađana and Beskoski, Vladimir P. and Radotić, Ksenija",
year = "2019",
abstract = "Reports about the influence of cerium-oxide nanoparticles (nCeO(2)) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO(2) with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO(2) were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO(2) on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL(-1) of nCeO(2) on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO(2) was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO(2) had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development",
number = "11",
volume = "8",
doi = "10.3390/plants8110478"
}

Milenković, I., Mitrović, A. Lj., Algarra, M., Lazaro-Martinez, J. M., Rodriguez-Castellon, E., Maksimović, V., Spasić, S., Beskoski, V. P.,& Radotić, K.. (2019). Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. in Plants-Basel
MDPI, Basel., 8(11).
https://doi.org/10.3390/plants8110478

Milenković I, Mitrović AL, Algarra M, Lazaro-Martinez JM, Rodriguez-Castellon E, Maksimović V, Spasić S, Beskoski VP, Radotić K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. in Plants-Basel. 2019;8(11).
doi:10.3390/plants8110478 .

Milenković, Ivana, Mitrović, Aleksandra Lj., Algarra, Manuel, Lazaro-Martinez, Juan M., Rodriguez-Castellon, Enrique, Maksimović, Vuk, Spasić, Slađana, Beskoski, Vladimir P., Radotić, Ksenija, "Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development" in Plants-Basel, 8, no. 11 (2019),
https://doi.org/10.3390/plants8110478 . .

TY  - JOUR
AU  - Zilic, Sladana
AU  - Dodig, Dejan
AU  - Vancetović, Jelena
AU  - Grcic, Nikola
AU  - Peric, Vesna
AU  - Titan, Primoz
AU  - Maksimović, Vuk
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1264
AB  - Colored grains are rich sources of anthocyanins that could play an important role in the prevention of various diseases associated with oxidative stress. Bearing in mind that cereals are widely grown crops, anthocyanins-rich colored grains could be used as a functional food ingredient that provides health benefits to a large part of human world's population. This study investigated composition and content of anthocyanins in the grain of blue popping maize, deep purple maize, purple wheat, and black soybean. The relationship of acylated and non-acylated forms before and after alkaline hydrolysis of anthocyanin extracts has been studied as well. Deep purple maize had the highest content of total anthocyanins reaching as much as 4988.90 mg CGE/kg d.m. Ten anthocyanins were identified in blue popping maize, of which two are isomers of cyanidin-3-(malonylglucoside) and three of cyanidin-3-(dimalonyl-beta-glucoside). Seven, nine, and three anthocyanins have been identified in the deep purple maize, purple wheat, and black soybean, respectively. Cyanidin derivatives were predominant and their acylated forms accounted for about 98, 29, 71, and 0% of the total anthocyanins content in the grains, respectively. According to the study, acylated derivatives were completely degraded under the effect of highly alkaline pH. However, at the beginning of their degradation they were transformed to their non-acylated parents. The results could be useful to better understanding of the nature of anthocyanin in colored grains and, in that regard, their use for the derivation of food products with functional potential, as well as of natural dyes and pharmaceutical ingredients.
PB  - Inst Animal Reproduction & Food Research Polish Acad Sciences Olsztyn, Olsztyn
T2  - Polish Journal of Food and Nutrition Sciences
T1  - Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives
EP  - 146
IS  - 2
SP  - 137
VL  - 69
DO  - 10.31883/pjfns-2019-105100
ER  - 

@article{
author = "Zilic, Sladana and Dodig, Dejan and Vancetović, Jelena and Grcic, Nikola and Peric, Vesna and Titan, Primoz and Maksimović, Vuk",
year = "2019",
abstract = "Colored grains are rich sources of anthocyanins that could play an important role in the prevention of various diseases associated with oxidative stress. Bearing in mind that cereals are widely grown crops, anthocyanins-rich colored grains could be used as a functional food ingredient that provides health benefits to a large part of human world's population. This study investigated composition and content of anthocyanins in the grain of blue popping maize, deep purple maize, purple wheat, and black soybean. The relationship of acylated and non-acylated forms before and after alkaline hydrolysis of anthocyanin extracts has been studied as well. Deep purple maize had the highest content of total anthocyanins reaching as much as 4988.90 mg CGE/kg d.m. Ten anthocyanins were identified in blue popping maize, of which two are isomers of cyanidin-3-(malonylglucoside) and three of cyanidin-3-(dimalonyl-beta-glucoside). Seven, nine, and three anthocyanins have been identified in the deep purple maize, purple wheat, and black soybean, respectively. Cyanidin derivatives were predominant and their acylated forms accounted for about 98, 29, 71, and 0% of the total anthocyanins content in the grains, respectively. According to the study, acylated derivatives were completely degraded under the effect of highly alkaline pH. However, at the beginning of their degradation they were transformed to their non-acylated parents. The results could be useful to better understanding of the nature of anthocyanin in colored grains and, in that regard, their use for the derivation of food products with functional potential, as well as of natural dyes and pharmaceutical ingredients.",
publisher = "Inst Animal Reproduction & Food Research Polish Acad Sciences Olsztyn, Olsztyn",
journal = "Polish Journal of Food and Nutrition Sciences",
title = "Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives",
pages = "146-137",
number = "2",
volume = "69",
doi = "10.31883/pjfns-2019-105100"
}

Zilic, S., Dodig, D., Vancetović, J., Grcic, N., Peric, V., Titan, P.,& Maksimović, V.. (2019). Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives. in Polish Journal of Food and Nutrition Sciences
Inst Animal Reproduction & Food Research Polish Acad Sciences Olsztyn, Olsztyn., 69(2), 137-146.
https://doi.org/10.31883/pjfns-2019-105100

Zilic S, Dodig D, Vancetović J, Grcic N, Peric V, Titan P, Maksimović V. Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives. in Polish Journal of Food and Nutrition Sciences. 2019;69(2):137-146.
doi:10.31883/pjfns-2019-105100 .

Zilic, Sladana, Dodig, Dejan, Vancetović, Jelena, Grcic, Nikola, Peric, Vesna, Titan, Primoz, Maksimović, Vuk, "Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives" in Polish Journal of Food and Nutrition Sciences, 69, no. 2 (2019):137-146,
https://doi.org/10.31883/pjfns-2019-105100 . .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragicević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Misic, Danijela
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1244
AB  - Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.
EP  - 94
SP  - 87
VL  - 237
DO  - 10.1016/j.jplph.2019.04.006
ER  - 

@article{
author = "Dmitrović, Slavica and Dragicević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Misic, Danijela",
year = "2019",
abstract = "Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.",
pages = "94-87",
volume = "237",
doi = "10.1016/j.jplph.2019.04.006"
}

Dmitrović, S., Dragicević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D.,& Misic, D.. (2019). Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 237, 87-94.
https://doi.org/10.1016/j.jplph.2019.04.006

Dmitrović S, Dragicević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Misic D. Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology. 2019;237:87-94.
doi:10.1016/j.jplph.2019.04.006 .

Dmitrović, Slavica, Dragicević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Misic, Danijela, "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh." in Journal of Plant Physiology, 237 (2019):87-94,
https://doi.org/10.1016/j.jplph.2019.04.006 . .

TY  - JOUR
AU  - Karaman, Maja
AU  - Atlagic, Kristina
AU  - Novaković, Aleksandra
AU  - Sibul, Filip
AU  - Zivić, Miroslav
AU  - Stevanović, Katarina
AU  - Pejin, Boris
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1198
AB  - Compared to plants, nowadays mushrooms attract more attention as functional foods, due to a number of advantages in manipulating them. This study aimed to screen the chemical composition (fatty acids and phenolics) and antioxidant potential (OH center dot, 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) and ferric reducing ability of plasma (FRAP)) of two edible mushrooms, Coprinus comatus and Coprinellus truncorum, collected from nature and submerged cultivation. Partial least square regression analysis has pointed out the importance of some fatty acids-more precisely, unsaturated fatty acids (UFAs) followed by fatty acids possessing both short (C6:0 and C8:0) and long (C23:0 and C24:0) saturated chains-and phenolic compounds (such as protocatechuic acid, daidzein, p-hydroxybenzoic acid, genistein and vanillic acid) for promising anti-OH center dot, FRAP and anti-DPPH center dot activities, respectively. However, other fatty acids (C16:0, C18:0 and C18:3n3) along with the flavonol isorhamnetin are actually suspected to negatively affect (by acting pro-oxidative) the aforementioned parameters, respectively. Taken together, design of new food supplements targeting oxidative stress might be predominantly based on the various UFAs combinations (C18:2n6, C20:1, C20:2, C20:4n6, C22:2, C22:1n9, etc.), particularly if OH center dot is suspected to play an important role.
PB  - MDPI, Basel
T2  - Antioxidants
T1  - Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms
IS  - 10
VL  - 8
DO  - 10.3390/antiox8100480
ER  - 

@article{
author = "Karaman, Maja and Atlagic, Kristina and Novaković, Aleksandra and Sibul, Filip and Zivić, Miroslav and Stevanović, Katarina and Pejin, Boris",
year = "2019",
abstract = "Compared to plants, nowadays mushrooms attract more attention as functional foods, due to a number of advantages in manipulating them. This study aimed to screen the chemical composition (fatty acids and phenolics) and antioxidant potential (OH center dot, 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) and ferric reducing ability of plasma (FRAP)) of two edible mushrooms, Coprinus comatus and Coprinellus truncorum, collected from nature and submerged cultivation. Partial least square regression analysis has pointed out the importance of some fatty acids-more precisely, unsaturated fatty acids (UFAs) followed by fatty acids possessing both short (C6:0 and C8:0) and long (C23:0 and C24:0) saturated chains-and phenolic compounds (such as protocatechuic acid, daidzein, p-hydroxybenzoic acid, genistein and vanillic acid) for promising anti-OH center dot, FRAP and anti-DPPH center dot activities, respectively. However, other fatty acids (C16:0, C18:0 and C18:3n3) along with the flavonol isorhamnetin are actually suspected to negatively affect (by acting pro-oxidative) the aforementioned parameters, respectively. Taken together, design of new food supplements targeting oxidative stress might be predominantly based on the various UFAs combinations (C18:2n6, C20:1, C20:2, C20:4n6, C22:2, C22:1n9, etc.), particularly if OH center dot is suspected to play an important role.",
publisher = "MDPI, Basel",
journal = "Antioxidants",
title = "Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms",
number = "10",
volume = "8",
doi = "10.3390/antiox8100480"
}

Karaman, M., Atlagic, K., Novaković, A., Sibul, F., Zivić, M., Stevanović, K.,& Pejin, B.. (2019). Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms. in Antioxidants
MDPI, Basel., 8(10).
https://doi.org/10.3390/antiox8100480

Karaman M, Atlagic K, Novaković A, Sibul F, Zivić M, Stevanović K, Pejin B. Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms. in Antioxidants. 2019;8(10).
doi:10.3390/antiox8100480 .

Karaman, Maja, Atlagic, Kristina, Novaković, Aleksandra, Sibul, Filip, Zivić, Miroslav, Stevanović, Katarina, Pejin, Boris, "Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms" in Antioxidants, 8, no. 10 (2019),
https://doi.org/10.3390/antiox8100480 . .

TY  - GEN
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2210
AB  - Vanadijum je esencijalni mikroelement za mnoge žive organizme i učesnik je mnogobrojnih bioloških procesa kod njih. načajnost u ispitivanju ovog elementa porasla je naglo u poslednje tri decenije pretpostavkom o njegovom značajnom doprinosu u tretmanu raznih vrsta bolesti. Skoro sve potrebne informacije koje se tiču oksidacionog oblika, koordinacione geometrije, oligomernog stanja vanadijuma kao i kako prisustvo različitih substrata inhibitora enzimskih aktivnosti utiču na njegove karakteristike se mogu dobiti upotrebom i kombinacijom različitih spektroskopskih metoda. Ispitivanje promena strukture vanadijuma kao potencijalno toksičnog ali i metabolički korisnog elementa je našlo veliku primenu u ispitivanju njegovog metabolizma kod gljiva kao vrste organizama koji imaju sposobnost usvajanja i modulacije različitih vrsta elemenata. Različite vrste gljiva usvajaju vanadijum u različitim oblicima, metabolišu ga na različite načine i imaju različite odgovore na njegovo prisustvo. bog složenih fiziko-hemijskih karakteristika i osetljivosti na uslove u kojima se nalazi, ispitivanje vanadijuma zahteva istovremenu primenu više spektroskopskih tehnika. Primena NMR, EPR, Ramanske, IC, optičke i Rendgenske spektroskopije u izučavanju ovog elementa kod gljiva rasvetljava njegovu ulogu u živim sistemima i daje nam informacije o mogućnostima biotransformacije ovog elementa što može imati značajnu ulogu u mnogim granama nauke a pre svega u medicini i zaštiti životne sredine.
PB  - Srpsko biološko društvo, Beograd
T2  - DRUGI KONGRES BIOLOGA SRBIJE
T1  - Biofizički pristup u rasvetljavanju metabolizma vanadijuma kod gljiva
EP  - 16
SP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2210
ER  - 

@misc{
author = "Žižić, Milan and Stanić, Marina and Živić, Miroslav and Zakrzewska, Joanna",
year = "2018",
abstract = "Vanadijum je esencijalni mikroelement za mnoge žive organizme i učesnik je mnogobrojnih bioloških procesa kod njih. načajnost u ispitivanju ovog elementa porasla je naglo u poslednje tri decenije pretpostavkom o njegovom značajnom doprinosu u tretmanu raznih vrsta bolesti. Skoro sve potrebne informacije koje se tiču oksidacionog oblika, koordinacione geometrije, oligomernog stanja vanadijuma kao i kako prisustvo različitih substrata inhibitora enzimskih aktivnosti utiču na njegove karakteristike se mogu dobiti upotrebom i kombinacijom različitih spektroskopskih metoda. Ispitivanje promena strukture vanadijuma kao potencijalno toksičnog ali i metabolički korisnog elementa je našlo veliku primenu u ispitivanju njegovog metabolizma kod gljiva kao vrste organizama koji imaju sposobnost usvajanja i modulacije različitih vrsta elemenata. Različite vrste gljiva usvajaju vanadijum u različitim oblicima, metabolišu ga na različite načine i imaju različite odgovore na njegovo prisustvo. bog složenih fiziko-hemijskih karakteristika i osetljivosti na uslove u kojima se nalazi, ispitivanje vanadijuma zahteva istovremenu primenu više spektroskopskih tehnika. Primena NMR, EPR, Ramanske, IC, optičke i Rendgenske spektroskopije u izučavanju ovog elementa kod gljiva rasvetljava njegovu ulogu u živim sistemima i daje nam informacije o mogućnostima biotransformacije ovog elementa što može imati značajnu ulogu u mnogim granama nauke a pre svega u medicini i zaštiti životne sredine.",
publisher = "Srpsko biološko društvo, Beograd",
journal = "DRUGI KONGRES BIOLOGA SRBIJE",
title = "Biofizički pristup u rasvetljavanju metabolizma vanadijuma kod gljiva",
pages = "16-16",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2210"
}

Žižić, M., Stanić, M., Živić, M.,& Zakrzewska, J.. (2018). Biofizički pristup u rasvetljavanju metabolizma vanadijuma kod gljiva. in DRUGI KONGRES BIOLOGA SRBIJE
Srpsko biološko društvo, Beograd., 16-16.
https://hdl.handle.net/21.15107/rcub_rimsi_2210

Žižić M, Stanić M, Živić M, Zakrzewska J. Biofizički pristup u rasvetljavanju metabolizma vanadijuma kod gljiva. in DRUGI KONGRES BIOLOGA SRBIJE. 2018;:16-16.
https://hdl.handle.net/21.15107/rcub_rimsi_2210 .

Žižić, Milan, Stanić, Marina, Živić, Miroslav, Zakrzewska, Joanna, "Biofizički pristup u rasvetljavanju metabolizma vanadijuma kod gljiva" in DRUGI KONGRES BIOLOGA SRBIJE (2018):16-16,
https://hdl.handle.net/21.15107/rcub_rimsi_2210 .

TY  - CONF
AU  - Žižić, Milan
AU  - Tešanović, Kristina
AU  - Karaman, Maja
AU  - Zakrzewska, Joanna
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2207
AB  - Laetiporus sulphureus (Bull.) Murrill 1920, (Ph. Basidiomycota, Cl. Agaricomycetes, O. Polyporales, Fam. Polyporaceae) je jestiva gljiva, široko rasprostranjena u Evropi, Aziji i Severnoj Americi. Izolovana micelija autohtone gljive (vrba obala Dunava, Novi Sad) kultivisana je na čvrstoj podlozi i u potopljenom medijumu. Cilj istraživanja bio je da se upotrebom NMR spektroskopije ispita uticaj vanadijuma na metabolizam fosfata. Utvrđeno je da signali pozicionirani oko 4 ppm i 2,6 ppm pripadaju šećernim fosfatima (SP) i da nakon tretmana sa 10 mM vanadatom dolazi do povećanja signala za 78%, odnosno 23%. Nakon ispiranja micelijuma dolazi do postepene transformacije jednog oblika u drugi, tako da posle sat vremena signal SP na 2,6 ppm postaje dominantan u odnosu na signal pozicioniran na 4 ppm. Promene signala SP mogu ukazati na efektivnost inhibitora ili stimulatora energetskog metabolizma. Pored SP, polifosfati (PolyP) prisutni u miceliji gljive, takođe imaju ulogu u skladištenju energije. Primećeno je da se nakon izlaganja nepovoljnjim uslovima signal PolyP smanjio, što je potvrđeno u studijama sa kvascima S. cereviseae1 i N. crassa2. Nasuprot tome, nakon tretmana vanadijumom kod L. sulphureus nije došlo do redukcije signala PolyP, već je zabeležen mali porast signala dugolančanih polifosfata što može ukazati na mehanizam detoksifikacije micelije L. sulphureus kao metaboličkog odgovora micelije na dodavanje vanadata.
PB  - Srpsko biološko društvo, Beograd
C3  - DRUGI KONGRES BIOLOGA SRBIJE
T1  - Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus
EP  - 36
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2207
ER  - 

@conference{
author = "Žižić, Milan and Tešanović, Kristina and Karaman, Maja and Zakrzewska, Joanna",
year = "2018",
abstract = "Laetiporus sulphureus (Bull.) Murrill 1920, (Ph. Basidiomycota, Cl. Agaricomycetes, O. Polyporales, Fam. Polyporaceae) je jestiva gljiva, široko rasprostranjena u Evropi, Aziji i Severnoj Americi. Izolovana micelija autohtone gljive (vrba obala Dunava, Novi Sad) kultivisana je na čvrstoj podlozi i u potopljenom medijumu. Cilj istraživanja bio je da se upotrebom NMR spektroskopije ispita uticaj vanadijuma na metabolizam fosfata. Utvrđeno je da signali pozicionirani oko 4 ppm i 2,6 ppm pripadaju šećernim fosfatima (SP) i da nakon tretmana sa 10 mM vanadatom dolazi do povećanja signala za 78%, odnosno 23%. Nakon ispiranja micelijuma dolazi do postepene transformacije jednog oblika u drugi, tako da posle sat vremena signal SP na 2,6 ppm postaje dominantan u odnosu na signal pozicioniran na 4 ppm. Promene signala SP mogu ukazati na efektivnost inhibitora ili stimulatora energetskog metabolizma. Pored SP, polifosfati (PolyP) prisutni u miceliji gljive, takođe imaju ulogu u skladištenju energije. Primećeno je da se nakon izlaganja nepovoljnjim uslovima signal PolyP smanjio, što je potvrđeno u studijama sa kvascima S. cereviseae1 i N. crassa2. Nasuprot tome, nakon tretmana vanadijumom kod L. sulphureus nije došlo do redukcije signala PolyP, već je zabeležen mali porast signala dugolančanih polifosfata što može ukazati na mehanizam detoksifikacije micelije L. sulphureus kao metaboličkog odgovora micelije na dodavanje vanadata.",
publisher = "Srpsko biološko društvo, Beograd",
journal = "DRUGI KONGRES BIOLOGA SRBIJE",
title = "Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus",
pages = "36-36",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2207"
}

Žižić, M., Tešanović, K., Karaman, M.,& Zakrzewska, J.. (2018). Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus. in DRUGI KONGRES BIOLOGA SRBIJE
Srpsko biološko društvo, Beograd., 36-36.
https://hdl.handle.net/21.15107/rcub_rimsi_2207

Žižić M, Tešanović K, Karaman M, Zakrzewska J. Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus. in DRUGI KONGRES BIOLOGA SRBIJE. 2018;:36-36.
https://hdl.handle.net/21.15107/rcub_rimsi_2207 .

Žižić, Milan, Tešanović, Kristina, Karaman, Maja, Zakrzewska, Joanna, "Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus" in DRUGI KONGRES BIOLOGA SRBIJE (2018):36-36,
https://hdl.handle.net/21.15107/rcub_rimsi_2207 .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antič, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2205
AB  - Vanadijum je prelazni metal, koji se može naći u brojnim oksidacionim stanjima, pri čemu su najznačajnija +4 (vanadil) i +5 (vanadat). S obzirom da gljive apsorbuju vanadijum, one predstavljaju glavni put njegovog ulaska u ekosistem.1 Vanadat je izučavan kao potencijalni antidijabetski i antikancerogeni agens, ali zbog svoje komplikovane hemije se ne koristi u terapijama. Mnogi efekti vanadijuma na ćeliju nisu poznati, a među njima je i efekat na sisteme antioksidativne zaštite.2 U ovom istraživanju je ispitivana aktivnost superoksid dismutaza (SOD), peroksidaza (Prx) i katalaza (CAT) tokom različitih faza razvoja gljive Phycomyces blakesleeanus, a kao odgovor na tretman micelijuma vanadatom. Efekat je praćen kod gljiva starosti 20 h, 36 h i 56 h, koje su izlagane 10 mM vanadatu tokom 1 h, 3 h ili 5 h. U eksponencijalnoj fazi razvoja (20 h) zabeležen je porast aktivnosti Prx i to 45±7% nakon 1 h, odnosno 19±7% nakon 5 h tretmana. Na prelasku iz eksponencijalne u stacionarnu fazu razvoja (36 h) 10 mM vanadat nije doveo do promene aktivnosti Prx u tretiranim uzorcima, dok je u stacionarnoj fazi razvoja (56 h) ponovo zabeležen porast aktivnosti Prx i to 22±4% nakon 1 h, odnosno 31±19% nakon 3 h tretmana. Porast aktivnosti SOD je zabeležen samo u stacionarnoj fazi razvoja, nakon 1 h tretmana i iznosi 26±2%. Nije uočena jasna promena aktivnosti CAT tretiranih u odnosu na kontrolne uzorke
PB  - Srpsko biološko društvo, Beograd
C3  - DRUGI KONGRES BIOLOGA SRBIJE
T1  - Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus
EP  - 33
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2205
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antič, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2018",
abstract = "Vanadijum je prelazni metal, koji se može naći u brojnim oksidacionim stanjima, pri čemu su najznačajnija +4 (vanadil) i +5 (vanadat). S obzirom da gljive apsorbuju vanadijum, one predstavljaju glavni put njegovog ulaska u ekosistem.1 Vanadat je izučavan kao potencijalni antidijabetski i antikancerogeni agens, ali zbog svoje komplikovane hemije se ne koristi u terapijama. Mnogi efekti vanadijuma na ćeliju nisu poznati, a među njima je i efekat na sisteme antioksidativne zaštite.2 U ovom istraživanju je ispitivana aktivnost superoksid dismutaza (SOD), peroksidaza (Prx) i katalaza (CAT) tokom različitih faza razvoja gljive Phycomyces blakesleeanus, a kao odgovor na tretman micelijuma vanadatom. Efekat je praćen kod gljiva starosti 20 h, 36 h i 56 h, koje su izlagane 10 mM vanadatu tokom 1 h, 3 h ili 5 h. U eksponencijalnoj fazi razvoja (20 h) zabeležen je porast aktivnosti Prx i to 45±7% nakon 1 h, odnosno 19±7% nakon 5 h tretmana. Na prelasku iz eksponencijalne u stacionarnu fazu razvoja (36 h) 10 mM vanadat nije doveo do promene aktivnosti Prx u tretiranim uzorcima, dok je u stacionarnoj fazi razvoja (56 h) ponovo zabeležen porast aktivnosti Prx i to 22±4% nakon 1 h, odnosno 31±19% nakon 3 h tretmana. Porast aktivnosti SOD je zabeležen samo u stacionarnoj fazi razvoja, nakon 1 h tretmana i iznosi 26±2%. Nije uočena jasna promena aktivnosti CAT tretiranih u odnosu na kontrolne uzorke",
publisher = "Srpsko biološko društvo, Beograd",
journal = "DRUGI KONGRES BIOLOGA SRBIJE",
title = "Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus",
pages = "33-33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2205"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetić Antič, T., Živić, M.,& Stanić, M.. (2018). Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus. in DRUGI KONGRES BIOLOGA SRBIJE
Srpsko biološko društvo, Beograd., 33-33.
https://hdl.handle.net/21.15107/rcub_rimsi_2205

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetić Antič T, Živić M, Stanić M. Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus. in DRUGI KONGRES BIOLOGA SRBIJE. 2018;:33-33.
https://hdl.handle.net/21.15107/rcub_rimsi_2205 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antič, Tijana, Živić, Miroslav, Stanić, Marina, "Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus" in DRUGI KONGRES BIOLOGA SRBIJE (2018):33-33,
https://hdl.handle.net/21.15107/rcub_rimsi_2205 .

TY  - CONF
AU  - Ivanka, Rodić
AU  - Žižić, Milan
AU  - Lukičić, Jovana
AU  - Zakrzewska, Joanna
AU  - Živić, Miroslav
AU  - Cvetic Antic, Tijana
AU  - Stanić, Marina
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2204
AB  - Elementarni selen je najmanje biotoksičan oblik selena u prirodi, dok selen-oksianjoni mogu biti toksični zbog značajne pokretljivosti i rastvorljivosti u vodi. Mikrobiološka redukcija selena +4 (selenit) u elementarni selen je stoga od presudnog značaja za smanjenje bioraspoloživosti ovog elementa.1 Visoka reaktivnost selen-oksianjona sa tiolnim grupama, kao i formiranje kiseoničnih radikala u reakciji sa glutationom implicira da oksidativni stres doprinosi toksičnosti selena.2 Tokom biološke redukcije selenita se proizvodi velika količina peroksida čime se indukuje ekspresija gena koji kodiraju enzime antioksidativnog metabolizma, što uključuje i enzime metabolizma glutationa.1 Micelijum gljive Phycomyces blakesleeanus star 24 sata je tretiran 10 mM rastvorom selenita. Uzorci su nakon određenih vremenskih intervala (1, 5, 10, 30, 60 minuta, 24 i 96 sati) ispirani i homogenizovani, nakon čega je meren sadržaj glutationa i specifična aktivnost enzima: peroksidaza (POD), katalaza (CAT), superoksid dismutaza (SOD), glutation peroksidaze (GPx), glutation-S-transferaze (GST) i glutation reduktaze (GR). Nakon dodavanja selenita utvrđen je pad količine ukupnog glutationa u micelijumu. Aktivnost POD i SOD je rasla do 60 minuta, nakon čega je opadala. Aktivnost CAT opadala je odmah po dodatku selenita. Najizraženija je bila promena aktivnosti GPx gde je zabeležen značajan porast, dok su promene aktivnosti GST i GR bile manje izražene
PB  - Srpsko biološko društvo, Beograd
C3  - DRUGI KONGRES BIOLOGA SRBIJE
T1  - Efekti selenita na metabolizam glutationa kod gljive Phycomyces blakesleeanus
EP  - 30
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2204
ER  - 

@conference{
author = "Ivanka, Rodić and Žižić, Milan and Lukičić, Jovana and Zakrzewska, Joanna and Živić, Miroslav and Cvetic Antic, Tijana and Stanić, Marina",
year = "2018",
abstract = "Elementarni selen je najmanje biotoksičan oblik selena u prirodi, dok selen-oksianjoni mogu biti toksični zbog značajne pokretljivosti i rastvorljivosti u vodi. Mikrobiološka redukcija selena +4 (selenit) u elementarni selen je stoga od presudnog značaja za smanjenje bioraspoloživosti ovog elementa.1 Visoka reaktivnost selen-oksianjona sa tiolnim grupama, kao i formiranje kiseoničnih radikala u reakciji sa glutationom implicira da oksidativni stres doprinosi toksičnosti selena.2 Tokom biološke redukcije selenita se proizvodi velika količina peroksida čime se indukuje ekspresija gena koji kodiraju enzime antioksidativnog metabolizma, što uključuje i enzime metabolizma glutationa.1 Micelijum gljive Phycomyces blakesleeanus star 24 sata je tretiran 10 mM rastvorom selenita. Uzorci su nakon određenih vremenskih intervala (1, 5, 10, 30, 60 minuta, 24 i 96 sati) ispirani i homogenizovani, nakon čega je meren sadržaj glutationa i specifična aktivnost enzima: peroksidaza (POD), katalaza (CAT), superoksid dismutaza (SOD), glutation peroksidaze (GPx), glutation-S-transferaze (GST) i glutation reduktaze (GR). Nakon dodavanja selenita utvrđen je pad količine ukupnog glutationa u micelijumu. Aktivnost POD i SOD je rasla do 60 minuta, nakon čega je opadala. Aktivnost CAT opadala je odmah po dodatku selenita. Najizraženija je bila promena aktivnosti GPx gde je zabeležen značajan porast, dok su promene aktivnosti GST i GR bile manje izražene",
publisher = "Srpsko biološko društvo, Beograd",
journal = "DRUGI KONGRES BIOLOGA SRBIJE",
title = "Efekti selenita na metabolizam glutationa kod gljive Phycomyces blakesleeanus",
pages = "30-30",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2204"
}

Ivanka, R., Žižić, M., Lukičić, J., Zakrzewska, J., Živić, M., Cvetic Antic, T.,& Stanić, M.. (2018). Efekti selenita na metabolizam glutationa kod gljive Phycomyces blakesleeanus. in DRUGI KONGRES BIOLOGA SRBIJE
Srpsko biološko društvo, Beograd., 30-30.
https://hdl.handle.net/21.15107/rcub_rimsi_2204

Ivanka R, Žižić M, Lukičić J, Zakrzewska J, Živić M, Cvetic Antic T, Stanić M. Efekti selenita na metabolizam glutationa kod gljive Phycomyces blakesleeanus. in DRUGI KONGRES BIOLOGA SRBIJE. 2018;:30-30.
https://hdl.handle.net/21.15107/rcub_rimsi_2204 .

Ivanka, Rodić, Žižić, Milan, Lukičić, Jovana, Zakrzewska, Joanna, Živić, Miroslav, Cvetic Antic, Tijana, Stanić, Marina, "Efekti selenita na metabolizam glutationa kod gljive Phycomyces blakesleeanus" in DRUGI KONGRES BIOLOGA SRBIJE (2018):30-30,
https://hdl.handle.net/21.15107/rcub_rimsi_2204 .

TY  - JOUR
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Tesanović, Kristina
AU  - Bosković, Eleonora
AU  - Nesović, Milica
AU  - Karaman, Maja
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1119
AB  - Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P-31 NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V-51 NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Effects of vanadate on the mycelium of edible fungus Coprinus comatus
EP  - 326
SP  - 320
VL  - 50
DO  - 10.1016/j.jtemb.2018.07.017
ER  - 

@article{
author = "Žižić, Milan and Zakrzewska, Joanna and Tesanović, Kristina and Bosković, Eleonora and Nesović, Milica and Karaman, Maja",
year = "2018",
abstract = "Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P-31 NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V-51 NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Effects of vanadate on the mycelium of edible fungus Coprinus comatus",
pages = "326-320",
volume = "50",
doi = "10.1016/j.jtemb.2018.07.017"
}

Žižić, M., Zakrzewska, J., Tesanović, K., Bosković, E., Nesović, M.,& Karaman, M.. (2018). Effects of vanadate on the mycelium of edible fungus Coprinus comatus. in Journal of Trace Elements in Medicine and Biology
Elsevier Gmbh, Munich., 50, 320-326.
https://doi.org/10.1016/j.jtemb.2018.07.017

Žižić M, Zakrzewska J, Tesanović K, Bosković E, Nesović M, Karaman M. Effects of vanadate on the mycelium of edible fungus Coprinus comatus. in Journal of Trace Elements in Medicine and Biology. 2018;50:320-326.
doi:10.1016/j.jtemb.2018.07.017 .

Žižić, Milan, Zakrzewska, Joanna, Tesanović, Kristina, Bosković, Eleonora, Nesović, Milica, Karaman, Maja, "Effects of vanadate on the mycelium of edible fungus Coprinus comatus" in Journal of Trace Elements in Medicine and Biology, 50 (2018):320-326,
https://doi.org/10.1016/j.jtemb.2018.07.017 . .

TY  - CONF
AU  - Stojanović, Milica
AU  - Maksimović, Vuk
AU  - Dragišić Maksimović, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1864
AB  - Lettuce (Lactuca sativa L.) belongs to a group of leafy vegetables commonly used as a salad 
in human nutrition. As minimally processed food product, it is an important source of vitamins, 
minerals and various antioxidative compounds. In our greenhouse experiment, two Salanova let tuce cultivars (‘Aquino‘ RZ- green and ‘Gaugin‘ RZ- red multi-leaf butterhead) were grown in the 
fertile soil during three successive seasons (autumn, winter and spring). The aim of the study was 
to examine the effect of different microbiological fertilizers (EM Aktiv, Vital Tricho and combination 
of EM Aktiv and Vital Tricho), cultivars and growing seasons on the total phenolic content and 
peroxidase activity. Our results showed that in all seasons red cultivar ‘Gaugin‘ had higher total 
phenolic content compared to green cultivar ‘Aquino‘. Their highest level was measured in au tumn with fertilizer Vital Tricho (549.14 µg GAE g-1 FW). The elevated phenolic status of red versus 
green cultivar was further strengthened by its higher level of peroxidase with the highest activity 
measured in spring with combination of fertilizers EM Aktiv and Vital Tricho (0.43 U mg-1 prot). The 
higher peroxidase activity and content of UV-absorbing phenolics, as plant-borne substrates of 
these enzymes, in the red cultivars indicate that the red leaves are better equipped to combat 
oxidative stress. Consequently, red cultivar ‘Gaugin‘, with increased synthesis of health-promoting 
antioxidants, could be considered as functional food in human diet.
PB  - Društvo za fiziologiju biljaka Srbije
PB  - Institut za biološka istraživanja “Siniša Stanković”
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Total phenolic content and peroxidase activity in Salanova lettuce
EP  - 119
SP  - 119
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1864
ER  - 

@conference{
author = "Stojanović, Milica and Maksimović, Vuk and Dragišić Maksimović, Jelena",
year = "2018",
abstract = "Lettuce (Lactuca sativa L.) belongs to a group of leafy vegetables commonly used as a salad 
in human nutrition. As minimally processed food product, it is an important source of vitamins, 
minerals and various antioxidative compounds. In our greenhouse experiment, two Salanova let tuce cultivars (‘Aquino‘ RZ- green and ‘Gaugin‘ RZ- red multi-leaf butterhead) were grown in the 
fertile soil during three successive seasons (autumn, winter and spring). The aim of the study was 
to examine the effect of different microbiological fertilizers (EM Aktiv, Vital Tricho and combination 
of EM Aktiv and Vital Tricho), cultivars and growing seasons on the total phenolic content and 
peroxidase activity. Our results showed that in all seasons red cultivar ‘Gaugin‘ had higher total 
phenolic content compared to green cultivar ‘Aquino‘. Their highest level was measured in au tumn with fertilizer Vital Tricho (549.14 µg GAE g-1 FW). The elevated phenolic status of red versus 
green cultivar was further strengthened by its higher level of peroxidase with the highest activity 
measured in spring with combination of fertilizers EM Aktiv and Vital Tricho (0.43 U mg-1 prot). The 
higher peroxidase activity and content of UV-absorbing phenolics, as plant-borne substrates of 
these enzymes, in the red cultivars indicate that the red leaves are better equipped to combat 
oxidative stress. Consequently, red cultivar ‘Gaugin‘, with increased synthesis of health-promoting 
antioxidants, could be considered as functional food in human diet.",
publisher = "Društvo za fiziologiju biljaka Srbije, Institut za biološka istraživanja “Siniša Stanković”",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Total phenolic content and peroxidase activity in Salanova lettuce",
pages = "119-119",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1864"
}

Stojanović, M., Maksimović, V.,& Dragišić Maksimović, J.. (2018). Total phenolic content and peroxidase activity in Salanova lettuce. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Društvo za fiziologiju biljaka Srbije., 119-119.
https://hdl.handle.net/21.15107/rcub_rimsi_1864

Stojanović M, Maksimović V, Dragišić Maksimović J. Total phenolic content and peroxidase activity in Salanova lettuce. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:119-119.
https://hdl.handle.net/21.15107/rcub_rimsi_1864 .

Stojanović, Milica, Maksimović, Vuk, Dragišić Maksimović, Jelena, "Total phenolic content and peroxidase activity in Salanova lettuce" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):119-119,
https://hdl.handle.net/21.15107/rcub_rimsi_1864 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Maksimović, Vuk
AU  - Mutavdžić, Dragosav
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1814
AB  - In this work, two non-invasive and independent methods for the
characterization of aflatoxin-stressed wheat seeds were applied.Aflatoxins
(AFs) are secondary toxic fungi metabolites, which exhibit hazardous effects
on plants (phytotoxic), as well as on humans and animals. First, for 2D EPR
imaging, we used 3-CP spin probe for the estimation of changes in the redox
state of the aflatoxin-stressed seed. On the other side, an excitation-emission
fluorescence approach was used to evaluate the spectral profiles of the key
biological molecules. We determined their areas and the ratio of the two
emission peaks at 355 nm (Ex 290) and 430 nm (Ex 350), corresponding to
protein and phenol emission, respectively. Our results showed that the ratio
of the two peaks (p/f) decreased in the presence of AFs. Both methods
revealed to be useful for the rapid, non-invasive assessment of the seeds'
stress state induced by AFs.
PB  - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3  - PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY
EP  - 526
SP  - 523
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1814
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Maksimović, Vuk and Mutavdžić, Dragosav and Radotić, Ksenija",
year = "2018",
abstract = "In this work, two non-invasive and independent methods for the
characterization of aflatoxin-stressed wheat seeds were applied.Aflatoxins
(AFs) are secondary toxic fungi metabolites, which exhibit hazardous effects
on plants (phytotoxic), as well as on humans and animals. First, for 2D EPR
imaging, we used 3-CP spin probe for the estimation of changes in the redox
state of the aflatoxin-stressed seed. On the other side, an excitation-emission
fluorescence approach was used to evaluate the spectral profiles of the key
biological molecules. We determined their areas and the ratio of the two
emission peaks at 355 nm (Ex 290) and 430 nm (Ex 350), corresponding to
protein and phenol emission, respectively. Our results showed that the ratio
of the two peaks (p/f) decreased in the presence of AFs. Both methods
revealed to be useful for the rapid, non-invasive assessment of the seeds'
stress state induced by AFs.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY",
pages = "526-523",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1814"
}

Bartolić, D., Stanković, M., Maksimović, V., Mutavdžić, D.,& Radotić, K.. (2018). NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY. in PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 523-526.
https://hdl.handle.net/21.15107/rcub_rimsi_1814

Bartolić D, Stanković M, Maksimović V, Mutavdžić D, Radotić K. NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY. in PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2018;:523-526.
https://hdl.handle.net/21.15107/rcub_rimsi_1814 .

Bartolić, Dragana, Stanković, Mira, Maksimović, Vuk, Mutavdžić, Dragosav, Radotić, Ksenija, "NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY" in PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2018):523-526,
https://hdl.handle.net/21.15107/rcub_rimsi_1814 .

TY  - CONF
AU  - Milosavljević, Dragica
AU  - Maksimović, Vuk
AU  - Milivojević, Jasminka M
AU  - Dragišić Maksimović, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1798
AB  - The delicious fruits of strawberry (Fragaria x ananassa Duch.), consumed in both fresh and pro­ cessed form, аге ап important source of health promoting  compounds.  Amongst  them, straw  ber ­ ries possess consideraЫe quantities of various phenolics. For this study, twelve strawberry cultivars,  9 June bearing ('Roxana; 'Arosa; 'Joly', 'Asia', 'АIЬа', 'Jeny', 'Laetitia; 'Garda; 'Premy'), and 3 ever-bearing types ('AIЬion','Capri' and'lrma'), were evaluated for anthocyanin, phenolic acid and ftavonoid con­  tent using  HPLC-MS.  Dominant  anthocyanin  form  in  all  cultivars  was  pelargonidin-3-glucoside with the highest values detected in 'Asia' and 'Joly' cultivars (487 and 464 µg -g 1 FW, respect ively). 'Joly' also stands out for acylated pelargonidin -3- gluco side and prunin content, since the highest values of pelargonidin-3-rutinoside and  ellagic  acid  were  detected  in 'Laetitia'  (33  and  43  µg -g 1 FW, resp ectively). Uppermost оп the ftavonoid list was 'Asia' which was the richest in p-coumaroyl hexose and its derivate (87 and 64 µg -g 1 FW, respectively), followed by'Joly' dominating in kaempferol 3-glucuronide and kaempferol 3-coumaroyl glucuronide .The identification of phenolic com­ pounds revealed some interesting differences correlated to  the  culti var:  June  bearing  cultivars 'Asia' and 'Joly' were distinguished from others as the richest in terms of identified phenolic com ­ pounds. Branding  the  cultivars  with  optimal  phenolic  composition  empowers  their  consumption for  human  health  and wellЬeing.
PB  - Serbian Plant Physiology Society
PB  - University of Belgrade, Institute for Biological Research “Siniša Stanković”
PB  - University of Belgrade, Faculty of Biology
C3  - 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society - Book of Abstracts
T1  - Phenolic profiles of June bearing and everbearing strawberries
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1798
ER  - 

@conference{
author = "Milosavljević, Dragica and Maksimović, Vuk and Milivojević, Jasminka M and Dragišić Maksimović, Jelena",
year = "2018",
abstract = "The delicious fruits of strawberry (Fragaria x ananassa Duch.), consumed in both fresh and pro­ cessed form, аге ап important source of health promoting  compounds.  Amongst  them, straw  ber ­ ries possess consideraЫe quantities of various phenolics. For this study, twelve strawberry cultivars,  9 June bearing ('Roxana; 'Arosa; 'Joly', 'Asia', 'АIЬа', 'Jeny', 'Laetitia; 'Garda; 'Premy'), and 3 ever-bearing types ('AIЬion','Capri' and'lrma'), were evaluated for anthocyanin, phenolic acid and ftavonoid con­  tent using  HPLC-MS.  Dominant  anthocyanin  form  in  all  cultivars  was  pelargonidin-3-glucoside with the highest values detected in 'Asia' and 'Joly' cultivars (487 and 464 µg -g 1 FW, respect ively). 'Joly' also stands out for acylated pelargonidin -3- gluco side and prunin content, since the highest values of pelargonidin-3-rutinoside and  ellagic  acid  were  detected  in 'Laetitia'  (33  and  43  µg -g 1 FW, resp ectively). Uppermost оп the ftavonoid list was 'Asia' which was the richest in p-coumaroyl hexose and its derivate (87 and 64 µg -g 1 FW, respectively), followed by'Joly' dominating in kaempferol 3-glucuronide and kaempferol 3-coumaroyl glucuronide .The identification of phenolic com­ pounds revealed some interesting differences correlated to  the  culti var:  June  bearing  cultivars 'Asia' and 'Joly' were distinguished from others as the richest in terms of identified phenolic com ­ pounds. Branding  the  cultivars  with  optimal  phenolic  composition  empowers  their  consumption for  human  health  and wellЬeing.",
publisher = "Serbian Plant Physiology Society, University of Belgrade, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology",
journal = "3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society - Book of Abstracts",
title = "Phenolic profiles of June bearing and everbearing strawberries",
pages = "113",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1798"
}

Milosavljević, D., Maksimović, V., Milivojević, J. M.,& Dragišić Maksimović, J.. (2018). Phenolic profiles of June bearing and everbearing strawberries. in 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society - Book of Abstracts
Serbian Plant Physiology Society., 113.
https://hdl.handle.net/21.15107/rcub_rimsi_1798

Milosavljević D, Maksimović V, Milivojević JM, Dragišić Maksimović J. Phenolic profiles of June bearing and everbearing strawberries. in 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society - Book of Abstracts. 2018;:113.
https://hdl.handle.net/21.15107/rcub_rimsi_1798 .

Milosavljević, Dragica, Maksimović, Vuk, Milivojević, Jasminka M, Dragišić Maksimović, Jelena, "Phenolic profiles of June bearing and everbearing strawberries" in 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society - Book of Abstracts (2018):113,
https://hdl.handle.net/21.15107/rcub_rimsi_1798 .

TY  - JOUR
AU  - Zeng, Fanrong
AU  - Shabala, Sergey
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Bonales-Alatorre, Edgar
AU  - Shabala, Lana
AU  - Yu, Min
AU  - Zhang, Guoping
AU  - Živanović, Branka  D.
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1173
AB  - Efforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii ("pigface"). We show that storage parenchyma cells of C. rossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs. 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+-ATPase operation and higher nonenzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of nonselective cation channels as a major path for Na+ entry. Patch-clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability was higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels, whereas in mesophyll, this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues.
PB  - Wiley, Hoboken
T2  - Plant Cell and Environment
T1  - Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi
EP  - 2667
IS  - 11
SP  - 2654
VL  - 41
DO  - 10.1111/pce.13391
ER  - 

@article{
author = "Zeng, Fanrong and Shabala, Sergey and Dragišić Maksimović, Jelena and Maksimović, Vuk and Bonales-Alatorre, Edgar and Shabala, Lana and Yu, Min and Zhang, Guoping and Živanović, Branka  D.",
year = "2018",
abstract = "Efforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii ("pigface"). We show that storage parenchyma cells of C. rossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs. 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+-ATPase operation and higher nonenzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of nonselective cation channels as a major path for Na+ entry. Patch-clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability was higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels, whereas in mesophyll, this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues.",
publisher = "Wiley, Hoboken",
journal = "Plant Cell and Environment",
title = "Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi",
pages = "2667-2654",
number = "11",
volume = "41",
doi = "10.1111/pce.13391"
}

Zeng, F., Shabala, S., Dragišić Maksimović, J., Maksimović, V., Bonales-Alatorre, E., Shabala, L., Yu, M., Zhang, G.,& Živanović, Branka  D.. (2018). Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi. in Plant Cell and Environment
Wiley, Hoboken., 41(11), 2654-2667.
https://doi.org/10.1111/pce.13391

Zeng F, Shabala S, Dragišić Maksimović J, Maksimović V, Bonales-Alatorre E, Shabala L, Yu M, Zhang G, Živanović, Branka  D.. Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi. in Plant Cell and Environment. 2018;41(11):2654-2667.
doi:10.1111/pce.13391 .

Zeng, Fanrong, Shabala, Sergey, Dragišić Maksimović, Jelena, Maksimović, Vuk, Bonales-Alatorre, Edgar, Shabala, Lana, Yu, Min, Zhang, Guoping, Živanović, Branka  D., "Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi" in Plant Cell and Environment, 41, no. 11 (2018):2654-2667,
https://doi.org/10.1111/pce.13391 . .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Spasojević, Dragica
AU  - Orlovska, Irina
AU  - Kozyrovska, Natalia
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Dmitrović, Svetlana
AU  - Matović, Branko
AU  - Tasić, Nikola
AU  - Maksimović, Vuk
AU  - Sosnin, Mikhail
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1157
AB  - Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing
EP  - 503
SP  - 494
VL  - 118
DO  - 10.1016/j.ijbiomac.2018.06.067
ER  - 

@article{
author = "Zmejkoski, Danica and Spasojević, Dragica and Orlovska, Irina and Kozyrovska, Natalia and Soković, Marina and Glamočlija, Jasmina and Dmitrović, Svetlana and Matović, Branko and Tasić, Nikola and Maksimović, Vuk and Sosnin, Mikhail and Radotić, Ksenija",
year = "2018",
abstract = "Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing",
pages = "503-494",
volume = "118",
doi = "10.1016/j.ijbiomac.2018.06.067"
}

Zmejkoski, D., Spasojević, D., Orlovska, I., Kozyrovska, N., Soković, M., Glamočlija, J., Dmitrović, S., Matović, B., Tasić, N., Maksimović, V., Sosnin, M.,& Radotić, K.. (2018). Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 118, 494-503.
https://doi.org/10.1016/j.ijbiomac.2018.06.067

Zmejkoski D, Spasojević D, Orlovska I, Kozyrovska N, Soković M, Glamočlija J, Dmitrović S, Matović B, Tasić N, Maksimović V, Sosnin M, Radotić K. Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules. 2018;118:494-503.
doi:10.1016/j.ijbiomac.2018.06.067 .

Zmejkoski, Danica, Spasojević, Dragica, Orlovska, Irina, Kozyrovska, Natalia, Soković, Marina, Glamočlija, Jasmina, Dmitrović, Svetlana, Matović, Branko, Tasić, Nikola, Maksimović, Vuk, Sosnin, Mikhail, Radotić, Ksenija, "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing" in International Journal of Biological Macromolecules, 118 (2018):494-503,
https://doi.org/10.1016/j.ijbiomac.2018.06.067 . .

TY  - JOUR
AU  - Živanović, Branka  D.
AU  - Ullrich, Kristian K.
AU  - Steffens, Bianka
AU  - Spasić, Slađana
AU  - Galland, Paul
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1164
AB  - The roles of fungal auxins in the regulation of elongation growth, photo-, and gravitropism are completely unknown. We analyzed the effects of exogenous IAA (indole-3-acetic acid), various synthetic auxins including 1-NAA (1-naphthaleneacctic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid), and the auxin transport inhibitor NPA (N-1-naphtylphtalamic acid) on the growth rate and bending of the unicellular sporangiophore of the zygomycete fungus. Phycomyces blakesleeanus. Sporangiophores that were submerged in an aqueous buffer responded to IAA with a sustained enhancement of the growth rate, while 1-NAA, 2,4-D. and NPA elicited an inhibition. In contrast, sporangiophores kept in air responded to IAA with a 20 to 40% decrease of the growth rate, while 1-NAA and NPA elicited an enhancement. The unilateral and local application of IAA in the growing zone of the sporangiophore elicited in 30 min a moderate negative tropic bending in wild type C2 and mutant C148madC, which was, however, partially masked by a concomitant avoidance response caused by the aqueous buffer. Auxin transport-related genes ubiquitous in plants were found in a BLAST search of the Phycomyces genome. They included members of the AUX1 (auxin influx carrier protein 1), PILS (PIN-LIKES, auxin transport facilitator protein), and ABCB (plant ATP-binding cassette transporter B) families while members of the PIN family were absent. Our observations imply that IAA represents an intrinsic element of the sensory transduction of Phycomyces and that its mode of action must very likely differ in several respects from that operating in plant.
PB  - Springer Wien, Wien
T2  - Protoplasma
T1  - The effect of auxin (indole-3-acetic acid) on the growth rate and tropism of the sporangiophore of Phycomyces blakesleeanus and identification of auxin-related genes
EP  - 1347
IS  - 5
SP  - 1331
VL  - 255
DO  - 10.1007/s00709-018-1232-2
ER  - 

@article{
author = "Živanović, Branka  D. and Ullrich, Kristian K. and Steffens, Bianka and Spasić, Slađana and Galland, Paul",
year = "2018",
abstract = "The roles of fungal auxins in the regulation of elongation growth, photo-, and gravitropism are completely unknown. We analyzed the effects of exogenous IAA (indole-3-acetic acid), various synthetic auxins including 1-NAA (1-naphthaleneacctic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid), and the auxin transport inhibitor NPA (N-1-naphtylphtalamic acid) on the growth rate and bending of the unicellular sporangiophore of the zygomycete fungus. Phycomyces blakesleeanus. Sporangiophores that were submerged in an aqueous buffer responded to IAA with a sustained enhancement of the growth rate, while 1-NAA, 2,4-D. and NPA elicited an inhibition. In contrast, sporangiophores kept in air responded to IAA with a 20 to 40% decrease of the growth rate, while 1-NAA and NPA elicited an enhancement. The unilateral and local application of IAA in the growing zone of the sporangiophore elicited in 30 min a moderate negative tropic bending in wild type C2 and mutant C148madC, which was, however, partially masked by a concomitant avoidance response caused by the aqueous buffer. Auxin transport-related genes ubiquitous in plants were found in a BLAST search of the Phycomyces genome. They included members of the AUX1 (auxin influx carrier protein 1), PILS (PIN-LIKES, auxin transport facilitator protein), and ABCB (plant ATP-binding cassette transporter B) families while members of the PIN family were absent. Our observations imply that IAA represents an intrinsic element of the sensory transduction of Phycomyces and that its mode of action must very likely differ in several respects from that operating in plant.",
publisher = "Springer Wien, Wien",
journal = "Protoplasma",
title = "The effect of auxin (indole-3-acetic acid) on the growth rate and tropism of the sporangiophore of Phycomyces blakesleeanus and identification of auxin-related genes",
pages = "1347-1331",
number = "5",
volume = "255",
doi = "10.1007/s00709-018-1232-2"
}

Živanović, Branka  D., Ullrich, K. K., Steffens, B., Spasić, S.,& Galland, P.. (2018). The effect of auxin (indole-3-acetic acid) on the growth rate and tropism of the sporangiophore of Phycomyces blakesleeanus and identification of auxin-related genes. in Protoplasma
Springer Wien, Wien., 255(5), 1331-1347.
https://doi.org/10.1007/s00709-018-1232-2

Živanović, Branka  D., Ullrich KK, Steffens B, Spasić S, Galland P. The effect of auxin (indole-3-acetic acid) on the growth rate and tropism of the sporangiophore of Phycomyces blakesleeanus and identification of auxin-related genes. in Protoplasma. 2018;255(5):1331-1347.
doi:10.1007/s00709-018-1232-2 .

Živanović, Branka  D., Ullrich, Kristian K., Steffens, Bianka, Spasić, Slađana, Galland, Paul, "The effect of auxin (indole-3-acetic acid) on the growth rate and tropism of the sporangiophore of Phycomyces blakesleeanus and identification of auxin-related genes" in Protoplasma, 255, no. 5 (2018):1331-1347,
https://doi.org/10.1007/s00709-018-1232-2 . .

TY  - JOUR
AU  - Jakovljevic-Uzelac, Jovana
AU  - Stanić, Marina
AU  - Krstic, Danijela
AU  - Colović, Mirjana
AU  - Djuric, Dragan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1152
AB  - The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.
PB  - Springer, Dordrecht
T2  - Molecular and Cellular Biochemistry
T1  - Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters
EP  - 148
IS  - 1-2
SP  - 143
VL  - 444
DO  - 10.1007/s11010-017-3238-z
ER  - 

@article{
author = "Jakovljevic-Uzelac, Jovana and Stanić, Marina and Krstic, Danijela and Colović, Mirjana and Djuric, Dragan",
year = "2018",
abstract = "The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.",
publisher = "Springer, Dordrecht",
journal = "Molecular and Cellular Biochemistry",
title = "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters",
pages = "148-143",
number = "1-2",
volume = "444",
doi = "10.1007/s11010-017-3238-z"
}

Jakovljevic-Uzelac, J., Stanić, M., Krstic, D., Colović, M.,& Djuric, D.. (2018). Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry
Springer, Dordrecht., 444(1-2), 143-148.
https://doi.org/10.1007/s11010-017-3238-z

Jakovljevic-Uzelac J, Stanić M, Krstic D, Colović M, Djuric D. Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry. 2018;444(1-2):143-148.
doi:10.1007/s11010-017-3238-z .

Jakovljevic-Uzelac, Jovana, Stanić, Marina, Krstic, Danijela, Colović, Mirjana, Djuric, Dragan, "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters" in Molecular and Cellular Biochemistry, 444, no. 1-2 (2018):143-148,
https://doi.org/10.1007/s11010-017-3238-z . .