TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1912
AB  - Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant 
for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also 
for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can 
be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most 
significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) 
causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing 
bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from 
different localities in Serbia, with the aim to examine their genetic features, through 
sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified 
with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained 
sequences were checked for homology with strains available in NCBI database. Multilocus 
sequence analysis (MLSA) was then performed to determine relatedness among the tested 
isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing 
99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided
into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates
from this host. These results could be connected with pathogen adaptation to winter oilseed
rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable
crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria.
This observation on Serbian Xcc isolates gives a significant input on genetic variation and
constitutes a highly informative sample of X. campestris diversity.
PB  - Serbian Genetic Society,  Belgrade, Serbia
C3  - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
T1  - GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE
SP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1912
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant 
for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also 
for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can 
be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most 
significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) 
causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing 
bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from 
different localities in Serbia, with the aim to examine their genetic features, through 
sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified 
with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained 
sequences were checked for homology with strains available in NCBI database. Multilocus 
sequence analysis (MLSA) was then performed to determine relatedness among the tested 
isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing 
99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided
into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates
from this host. These results could be connected with pathogen adaptation to winter oilseed
rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable
crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria.
This observation on Serbian Xcc isolates gives a significant input on genetic variation and
constitutes a highly informative sample of X. campestris diversity.",
publisher = "Serbian Genetic Society,  Belgrade, Serbia",
journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY",
title = "GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE",
pages = "163",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1912"
}

Jelušić, A., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
Serbian Genetic Society,  Belgrade, Serbia., 163.
https://hdl.handle.net/21.15107/rcub_rimsi_1912

Jelušić A, Berić T, Mitrović P, Marković S, Stanković S, Popović T. GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:163.
https://hdl.handle.net/21.15107/rcub_rimsi_1912 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):163,
https://hdl.handle.net/21.15107/rcub_rimsi_1912 .

TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1915
AB  - Blackleg disease, caused by pectolytic bacteria from genus Dickeya and Pectobacterium
is currently being one of the main concerns in potato production. Yield loss and reduced 
quality, visible through potato black rotting at the stem base, as well as whole plant decaying, 
makes potato cultivation less profitable. Blackleg disease outbreak was observed in potato 
field (cv. Lady Claire) in Bačka (northern Serbia) in June 2018. The percent of the infected 
plants, estimated during the vegetation season was 45%, with yield reduction of about 20%. 
In this study the causal pathogens were genetically characterized using multilocus sequence 
analysis (MLSA). DNA of the obtained isolates was amplified with primers made based on 
the sequences of five housekeeping genes - gapA, icdA, mdh, pgi and proA and sequenced. 
Phylogenetic analysis was performed to compare the obtained isolates with the ones already 
deposited in NCBI database. NCBI BLAST identified isolates as Dickeya dianthicola and 
Pectobacterium carotovorum subsp. brasiliensis, indicating on the persistence of mix infection 
on observed potato crop. According to all genes, isolates appeared to be the most similar 
(99-100%) to the reference strains - D. dianthicola (CFBP 1200) and P. carotovorum subsp. 
brasiliensis (BC1). Blast results were confirmed after phylogenetic analysis, where isolates 
of both identified species were clustering with the rest of the strains of the same species, 
obtained from NCBI. Our findings confirm the presence of new blackleg causal agents, D. 
dianthicola and P. carotovorum subsp. brasiliensis on potato in Serbia. Their isolation and 
localization is of great importance, especially D. dianthicola which has quarantine status, and 
therefore it gives a signal for pathogen eradication and prevention of its future spreading.
PB  - Serbian Genetic Society,  Belgrade, Serbia
C3  - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
T1  - GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO
SP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1915
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2019",
abstract = "Blackleg disease, caused by pectolytic bacteria from genus Dickeya and Pectobacterium
is currently being one of the main concerns in potato production. Yield loss and reduced 
quality, visible through potato black rotting at the stem base, as well as whole plant decaying, 
makes potato cultivation less profitable. Blackleg disease outbreak was observed in potato 
field (cv. Lady Claire) in Bačka (northern Serbia) in June 2018. The percent of the infected 
plants, estimated during the vegetation season was 45%, with yield reduction of about 20%. 
In this study the causal pathogens were genetically characterized using multilocus sequence 
analysis (MLSA). DNA of the obtained isolates was amplified with primers made based on 
the sequences of five housekeeping genes - gapA, icdA, mdh, pgi and proA and sequenced. 
Phylogenetic analysis was performed to compare the obtained isolates with the ones already 
deposited in NCBI database. NCBI BLAST identified isolates as Dickeya dianthicola and 
Pectobacterium carotovorum subsp. brasiliensis, indicating on the persistence of mix infection 
on observed potato crop. According to all genes, isolates appeared to be the most similar 
(99-100%) to the reference strains - D. dianthicola (CFBP 1200) and P. carotovorum subsp. 
brasiliensis (BC1). Blast results were confirmed after phylogenetic analysis, where isolates 
of both identified species were clustering with the rest of the strains of the same species, 
obtained from NCBI. Our findings confirm the presence of new blackleg causal agents, D. 
dianthicola and P. carotovorum subsp. brasiliensis on potato in Serbia. Their isolation and 
localization is of great importance, especially D. dianthicola which has quarantine status, and 
therefore it gives a signal for pathogen eradication and prevention of its future spreading.",
publisher = "Serbian Genetic Society,  Belgrade, Serbia",
journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY",
title = "GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO",
pages = "165",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1915"
}

Marković, S., Popović, T., Jelušić, A., Iličić, R.,& Stanković, S.. (2019). GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
Serbian Genetic Society,  Belgrade, Serbia., 165.
https://hdl.handle.net/21.15107/rcub_rimsi_1915

Marković S, Popović T, Jelušić A, Iličić R, Stanković S. GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:165.
https://hdl.handle.net/21.15107/rcub_rimsi_1915 .

Marković, Sanja, Popović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):165,
https://hdl.handle.net/21.15107/rcub_rimsi_1915 .

TY  - CONF
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1923
AB  - Ralstonia solanacearum is a soil borne bacterium which affects more than 450 plant species 
including a wide range of crop plants, ornamentals and weeds. This bacterium is causing 
bacterial wilt disease which leads to severe economic losses. On potato, disease is known 
as brown rot. Even though this phytopathogen originates from tropic, subtropics and warm 
temperate regions, in recent years cold-adapted strains dramatically enhanced the threat 
of European potato crops, including Serbia. R. solanacearum is at A2 list of quarantine plant 
pathogens in Europe. During six year period (2013-2018), isolates from diseased potato 
tubers were collected, identified using Multilocus sequence analysis (MLSA) and checked 
for certain phylotype affiliation. DNA from the obtained isolates was amplified using seven 
housekeeping genes (adk, fliC, gapA, gdhA, gyrB, hrpB, ppsA) and sequenced. Phylogenetic 
analysis was performed with concatenated sequences of all tested isolates, and compared 
with the most similar R. solanacearum strains from PAMDB database. All Serbian isolates 
were identified as R. solanacearum using PAMDB BLAST. Neighbor-joining phylogenetic 
analysis placed tested isolates in the same cluster with R. solanacearum strains belonging 
to race 3, biovar 2, and phylotype II obtained from PAMDB. Although, bacterial wilt caused 
by this plant pathogenic bacteria is appearing in different locations and on different potato 
cultivars in Serbia it remained genetically homogenous. Currently, R. solanacearum is a 
quarantine bacteria occurring only on potato in our country, but considering its wide host 
range, there is a possibility of it causing disease on other significant crops with devastating 
consequences. Therefore, its fast and accurate identification and mapping is of main interest 
for stopping the disease spread.
PB  - Serbian Genetic Society,  Belgrade, Serbia
C3  - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
T1  - MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA
SP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1923
ER  - 

@conference{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović, Tatjana",
year = "2019",
abstract = "Ralstonia solanacearum is a soil borne bacterium which affects more than 450 plant species 
including a wide range of crop plants, ornamentals and weeds. This bacterium is causing 
bacterial wilt disease which leads to severe economic losses. On potato, disease is known 
as brown rot. Even though this phytopathogen originates from tropic, subtropics and warm 
temperate regions, in recent years cold-adapted strains dramatically enhanced the threat 
of European potato crops, including Serbia. R. solanacearum is at A2 list of quarantine plant 
pathogens in Europe. During six year period (2013-2018), isolates from diseased potato 
tubers were collected, identified using Multilocus sequence analysis (MLSA) and checked 
for certain phylotype affiliation. DNA from the obtained isolates was amplified using seven 
housekeeping genes (adk, fliC, gapA, gdhA, gyrB, hrpB, ppsA) and sequenced. Phylogenetic 
analysis was performed with concatenated sequences of all tested isolates, and compared 
with the most similar R. solanacearum strains from PAMDB database. All Serbian isolates 
were identified as R. solanacearum using PAMDB BLAST. Neighbor-joining phylogenetic 
analysis placed tested isolates in the same cluster with R. solanacearum strains belonging 
to race 3, biovar 2, and phylotype II obtained from PAMDB. Although, bacterial wilt caused 
by this plant pathogenic bacteria is appearing in different locations and on different potato 
cultivars in Serbia it remained genetically homogenous. Currently, R. solanacearum is a 
quarantine bacteria occurring only on potato in our country, but considering its wide host 
range, there is a possibility of it causing disease on other significant crops with devastating 
consequences. Therefore, its fast and accurate identification and mapping is of main interest 
for stopping the disease spread.",
publisher = "Serbian Genetic Society,  Belgrade, Serbia",
journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY",
title = "MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA",
pages = "164",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1923"
}

Marković, S., Stanković, S., Jelušić, A., Iličić, R.,& Popović, T.. (2019). MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
Serbian Genetic Society,  Belgrade, Serbia., 164.
https://hdl.handle.net/21.15107/rcub_rimsi_1923

Marković S, Stanković S, Jelušić A, Iličić R, Popović T. MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:164.
https://hdl.handle.net/21.15107/rcub_rimsi_1923 .

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović, Tatjana, "MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):164,
https://hdl.handle.net/21.15107/rcub_rimsi_1923 .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Iličić, Renata
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1921
AB  - The causal agent of soft rot disease associated with a cabbage outbreak in Semberija 
region, Bosnia and Herzegovina, in 2018 was identified and characterized. Symptoms appeared 
in the form of water-soaked lesions on leaves and specific odour. Disease incidence ranged 
from 20% to 30%. The causal pathogen was isolated on nutrient agar (NA), King’s B and 
crystal violet pectate (CVP) media. Eight creamy-white, round and convex bacterial isolates, 
which produced characteristic pits on CVP medium were taken as representative. They were 
gram negative, facultative anaerobe, oxidase negative, catalase positive, nonfluorescent 
on King’s B medium, levan and arginine dehydrolase negative. The isolates were able to 
cause soft rot on cabbage and potato tuber slices 24 h after inoculation under conditions 
of high relative humidity. Polymerase chain reaction (PCR) was performed for preliminary 
identification by using three specific primer sets: F0145/E2477 (specific for Pectobacterium 
carotovorum subsp. carotovorum), Br1f/L1r (specific for P. carotovorum subsp. brasiliensis) and 
ECA1f/ECA2r (specific for P. atrosepticum). All isolates produced the band size of 666 bp with 
F0145/E2477 primer pair, indicating that they belong to the species P. carotovorum subsp. 
carotovorum. Further genetic characterization was based on sequence analysis of the gapA 
and mdh housekeeping genes. BLAST analysis confirmed 99.39% (Q. cover 100%, E. value 
0.0) and 100% (Q. cover 100%, E. value 0.0) identity of the isolates with P. carotovorum subsp. 
carotovorum strains deposited in the NCBI database as M34 (KY047594) for gapA and Pcc t0437 
(KC337296) for mdh genes, respectively. Phylogenetic analysis showed genetic homogeneity 
among the cabbage isolates.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent  outbreak on cabbage in Bosnia and Herzegovina
EP  - 222
IS  - 3-4
SP  - 211
VL  - 34
DO  - 10.2298/PIF1904211P
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Marković, Sanja and Iličić, Renata",
year = "2019",
abstract = "The causal agent of soft rot disease associated with a cabbage outbreak in Semberija 
region, Bosnia and Herzegovina, in 2018 was identified and characterized. Symptoms appeared 
in the form of water-soaked lesions on leaves and specific odour. Disease incidence ranged 
from 20% to 30%. The causal pathogen was isolated on nutrient agar (NA), King’s B and 
crystal violet pectate (CVP) media. Eight creamy-white, round and convex bacterial isolates, 
which produced characteristic pits on CVP medium were taken as representative. They were 
gram negative, facultative anaerobe, oxidase negative, catalase positive, nonfluorescent 
on King’s B medium, levan and arginine dehydrolase negative. The isolates were able to 
cause soft rot on cabbage and potato tuber slices 24 h after inoculation under conditions 
of high relative humidity. Polymerase chain reaction (PCR) was performed for preliminary 
identification by using three specific primer sets: F0145/E2477 (specific for Pectobacterium 
carotovorum subsp. carotovorum), Br1f/L1r (specific for P. carotovorum subsp. brasiliensis) and 
ECA1f/ECA2r (specific for P. atrosepticum). All isolates produced the band size of 666 bp with 
F0145/E2477 primer pair, indicating that they belong to the species P. carotovorum subsp. 
carotovorum. Further genetic characterization was based on sequence analysis of the gapA 
and mdh housekeeping genes. BLAST analysis confirmed 99.39% (Q. cover 100%, E. value 
0.0) and 100% (Q. cover 100%, E. value 0.0) identity of the isolates with P. carotovorum subsp. 
carotovorum strains deposited in the NCBI database as M34 (KY047594) for gapA and Pcc t0437 
(KC337296) for mdh genes, respectively. Phylogenetic analysis showed genetic homogeneity 
among the cabbage isolates.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent  outbreak on cabbage in Bosnia and Herzegovina",
pages = "222-211",
number = "3-4",
volume = "34",
doi = "10.2298/PIF1904211P"
}

Popović, T., Jelušić, A., Marković, S.,& Iličić, R.. (2019). Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent  outbreak on cabbage in Bosnia and Herzegovina. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 34(3-4), 211-222.
https://doi.org/10.2298/PIF1904211P

Popović T, Jelušić A, Marković S, Iličić R. Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent  outbreak on cabbage in Bosnia and Herzegovina. in Pesticidi i fitomedicina. 2019;34(3-4):211-222.
doi:10.2298/PIF1904211P .

Popović, Tatjana, Jelušić, Aleksandra, Marković, Sanja, Iličić, Renata, "Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent  outbreak on cabbage in Bosnia and Herzegovina" in Pesticidi i fitomedicina, 34, no. 3-4 (2019):211-222,
https://doi.org/10.2298/PIF1904211P . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Marković, Sanja
AU  - Ignjatov, Maja
AU  - Milovanović, Predrag
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1918
AB  - Brown rot or bacterial wilt caused by bacterium Ralstonia solanacearum is the main limiting factor in potato 
production. Quarantine measures are necessary to avoid spread of disease to disease-free areas. R. solanacearum has 
been shown to contaminate watercourses from which crop irrigation is then prohibited causing further potential losses 
in yield and quality. The bacteria also spread via surfaces that diseased seed potatoes come into contact with. This 
study showed bactericidal activity of chlorine dioxide (ClO2) on R. solanacearum for disinfection of water, surface and 
equipment. The results showed that ClO2 solution at concentration of 2 ppm at 30 minutes of exposure time had 
bactericidal effect for disinfection of water. For surface and equipment disinfection, concentration of 50 ppm showed 
total efficacy at 30 min and 5 sec exposure time, respectively. Results suggest that use of ClO2 as a disinfectant has a 
potential for control of brown rot pathogen in water, storage and equipment.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment
EP  - 84
IS  - 2
SP  - 81
VL  - 53
DO  - 10.5937/ratpov53-9982
ER  - 

@article{
author = "Popović, Tatjana and Ivanović, Žarko and Marković, Sanja and Ignjatov, Maja and Milovanović, Predrag",
year = "2016",
abstract = "Brown rot or bacterial wilt caused by bacterium Ralstonia solanacearum is the main limiting factor in potato 
production. Quarantine measures are necessary to avoid spread of disease to disease-free areas. R. solanacearum has 
been shown to contaminate watercourses from which crop irrigation is then prohibited causing further potential losses 
in yield and quality. The bacteria also spread via surfaces that diseased seed potatoes come into contact with. This 
study showed bactericidal activity of chlorine dioxide (ClO2) on R. solanacearum for disinfection of water, surface and 
equipment. The results showed that ClO2 solution at concentration of 2 ppm at 30 minutes of exposure time had 
bactericidal effect for disinfection of water. For surface and equipment disinfection, concentration of 50 ppm showed 
total efficacy at 30 min and 5 sec exposure time, respectively. Results suggest that use of ClO2 as a disinfectant has a 
potential for control of brown rot pathogen in water, storage and equipment.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment",
pages = "84-81",
number = "2",
volume = "53",
doi = "10.5937/ratpov53-9982"
}

Popović, T., Ivanović, Ž., Marković, S., Ignjatov, M.,& Milovanović, P.. (2016). Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 53(2), 81-84.
https://doi.org/10.5937/ratpov53-9982

Popović T, Ivanović Ž, Marković S, Ignjatov M, Milovanović P. Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2016;53(2):81-84.
doi:10.5937/ratpov53-9982 .

Popović, Tatjana, Ivanović, Žarko, Marković, Sanja, Ignjatov, Maja, Milovanović, Predrag, "Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 53, no. 2 (2016):81-84,
https://doi.org/10.5937/ratpov53-9982 . .