MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES ORIGINATED FROM POTATO IN SERBIA
2019
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Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ralstonia solanacearum is a soil borne bacterium which affects more than 450 plant species
including a wide range of crop plants, ornamentals and weeds. This bacterium is causing
bacterial wilt disease which leads to severe economic losses. On potato, disease is known
as brown rot. Even though this phytopathogen originates from tropic, subtropics and warm
temperate regions, in recent years cold-adapted strains dramatically enhanced the threat
of European potato crops, including Serbia. R. solanacearum is at A2 list of quarantine plant
pathogens in Europe. During six year period (2013-2018), isolates from diseased potato
tubers were collected, identified using Multilocus sequence analysis (MLSA) and checked
for certain phylotype affiliation. DNA from the obtained isolates was amplified using seven
housekeeping genes (adk, fliC, gapA, gdhA, gyrB, hrpB, ppsA) and sequenced. Phylogenetic
analysis was performed with concatenated sequences of all tested isolates, and comp...ared
with the most similar R. solanacearum strains from PAMDB database. All Serbian isolates
were identified as R. solanacearum using PAMDB BLAST. Neighbor-joining phylogenetic
analysis placed tested isolates in the same cluster with R. solanacearum strains belonging
to race 3, biovar 2, and phylotype II obtained from PAMDB. Although, bacterial wilt caused
by this plant pathogenic bacteria is appearing in different locations and on different potato
cultivars in Serbia it remained genetically homogenous. Currently, R. solanacearum is a
quarantine bacteria occurring only on potato in our country, but considering its wide host
range, there is a possibility of it causing disease on other significant crops with devastating
consequences. Therefore, its fast and accurate identification and mapping is of main interest
for stopping the disease spread.
Кључне речи:
Ralstonia solanacearum / bacterial wilt / brown rot / multilocus sequence analysis (mlsa)Извор:
6th CONGRESS OF THE SERBIAN GENETIC SOCIETY, 2019, 164-Издавач:
- Serbian Genetic Society, Belgrade, Serbia
Финансирање / пројекти:
- Ministry of Education, Science and Technological Development, Republic of Serbia (Project No. III43010)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Marković, Sanja AU - Stanković, Slaviša AU - Jelušić, Aleksandra AU - Iličić, Renata AU - Popović, Tatjana PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1923 AB - Ralstonia solanacearum is a soil borne bacterium which affects more than 450 plant species including a wide range of crop plants, ornamentals and weeds. This bacterium is causing bacterial wilt disease which leads to severe economic losses. On potato, disease is known as brown rot. Even though this phytopathogen originates from tropic, subtropics and warm temperate regions, in recent years cold-adapted strains dramatically enhanced the threat of European potato crops, including Serbia. R. solanacearum is at A2 list of quarantine plant pathogens in Europe. During six year period (2013-2018), isolates from diseased potato tubers were collected, identified using Multilocus sequence analysis (MLSA) and checked for certain phylotype affiliation. DNA from the obtained isolates was amplified using seven housekeeping genes (adk, fliC, gapA, gdhA, gyrB, hrpB, ppsA) and sequenced. Phylogenetic analysis was performed with concatenated sequences of all tested isolates, and compared with the most similar R. solanacearum strains from PAMDB database. All Serbian isolates were identified as R. solanacearum using PAMDB BLAST. Neighbor-joining phylogenetic analysis placed tested isolates in the same cluster with R. solanacearum strains belonging to race 3, biovar 2, and phylotype II obtained from PAMDB. Although, bacterial wilt caused by this plant pathogenic bacteria is appearing in different locations and on different potato cultivars in Serbia it remained genetically homogenous. Currently, R. solanacearum is a quarantine bacteria occurring only on potato in our country, but considering its wide host range, there is a possibility of it causing disease on other significant crops with devastating consequences. Therefore, its fast and accurate identification and mapping is of main interest for stopping the disease spread. PB - Serbian Genetic Society, Belgrade, Serbia C3 - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY T1 - MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES ORIGINATED FROM POTATO IN SERBIA SP - 164 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1923 ER -
@conference{ author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović, Tatjana", year = "2019", abstract = "Ralstonia solanacearum is a soil borne bacterium which affects more than 450 plant species including a wide range of crop plants, ornamentals and weeds. This bacterium is causing bacterial wilt disease which leads to severe economic losses. On potato, disease is known as brown rot. Even though this phytopathogen originates from tropic, subtropics and warm temperate regions, in recent years cold-adapted strains dramatically enhanced the threat of European potato crops, including Serbia. R. solanacearum is at A2 list of quarantine plant pathogens in Europe. During six year period (2013-2018), isolates from diseased potato tubers were collected, identified using Multilocus sequence analysis (MLSA) and checked for certain phylotype affiliation. DNA from the obtained isolates was amplified using seven housekeeping genes (adk, fliC, gapA, gdhA, gyrB, hrpB, ppsA) and sequenced. Phylogenetic analysis was performed with concatenated sequences of all tested isolates, and compared with the most similar R. solanacearum strains from PAMDB database. All Serbian isolates were identified as R. solanacearum using PAMDB BLAST. Neighbor-joining phylogenetic analysis placed tested isolates in the same cluster with R. solanacearum strains belonging to race 3, biovar 2, and phylotype II obtained from PAMDB. Although, bacterial wilt caused by this plant pathogenic bacteria is appearing in different locations and on different potato cultivars in Serbia it remained genetically homogenous. Currently, R. solanacearum is a quarantine bacteria occurring only on potato in our country, but considering its wide host range, there is a possibility of it causing disease on other significant crops with devastating consequences. Therefore, its fast and accurate identification and mapping is of main interest for stopping the disease spread.", publisher = "Serbian Genetic Society, Belgrade, Serbia", journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY", title = "MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES ORIGINATED FROM POTATO IN SERBIA", pages = "164", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1923" }
Marković, S., Stanković, S., Jelušić, A., Iličić, R.,& Popović, T.. (2019). MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES ORIGINATED FROM POTATO IN SERBIA. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY Serbian Genetic Society, Belgrade, Serbia., 164. https://hdl.handle.net/21.15107/rcub_rimsi_1923
Marković S, Stanković S, Jelušić A, Iličić R, Popović T. MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES ORIGINATED FROM POTATO IN SERBIA. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:164. https://hdl.handle.net/21.15107/rcub_rimsi_1923 .
Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović, Tatjana, "MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES ORIGINATED FROM POTATO IN SERBIA" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):164, https://hdl.handle.net/21.15107/rcub_rimsi_1923 .