TY  - JOUR
AU  - Bosnić, Predrag
AU  - Pavlicević, Milica
AU  - Nikolić, Nina
AU  - Nikolic, Miroslav
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1281
AB  - Both calcium (Ca2+) and silicon (Si) improve plant performance under salt (NaCl) stress. Although these two mineral elements share numerous similarities, the information on how their extracellular interactions in the root apoplast affect uptake of sodium (Na+) is still lacking. Here, we investigated the effect of high Si supply in the bioavailable form of monosilicic acid (H4SiO4) on the activity of Ca2+ in the external root solution, and subsequent root uptake and compartmentation of Na in maize (Zea mays L.). In the short-term experiments (6 h), 14-d-old maize plants were exposed to various concentrations of Ca2+ at three different pH-values (6.5, 7.5, and 8.5) and two Si concentrations, i.e., low (1 mM) and high (4 mM) supply of H4SiO4. The activity of Ca2+ and Na+ in the external solution as well as the root concentrations of total and cell sap and BaCl2-exchangeble apoplastic fractions of both elements were analyzed. The pH of the nutrient solution affected neither the ion activities nor the root accumulation of both Ca2+ and Na+. At higher pH values (7.5 and 8.5) the interactions of Ca2+ and Si at high Si supply led to a decrease of Ca2+ activity and, hence, an increase of Na+ : Ca2+ activity ratio in the external root solution. Concomitantly, despite the elevated exchangeable apoplastic fraction of both Ca2+ and Na+, the total and cell sap concentrations were remarkably decreased for Ca2+ and increased for Na+ by the addition of 4 mM H4SiO4. This work demonstrates that at high Si supply extracellular Ca-Si interactions leading to lowered activity of Ca2+ might rapidly compromise the ameliorative effect of Ca2+ on Na+ accumulation in roots. Practically, Si over-fertilization of saline and, in particular, sodic soils may further promote the accumulation of Na+ in root tissues hours after Si application and, hence, increase a potential risk of Na+ toxicity.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Journal of Plant Nutrition and Soil Science
T1  - High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity
EP  - 216
IS  - 2
SP  - 210
VL  - 182
DO  - 10.1002/jpln.201800153
ER  - 

@article{
author = "Bosnić, Predrag and Pavlicević, Milica and Nikolić, Nina and Nikolic, Miroslav",
year = "2019",
abstract = "Both calcium (Ca2+) and silicon (Si) improve plant performance under salt (NaCl) stress. Although these two mineral elements share numerous similarities, the information on how their extracellular interactions in the root apoplast affect uptake of sodium (Na+) is still lacking. Here, we investigated the effect of high Si supply in the bioavailable form of monosilicic acid (H4SiO4) on the activity of Ca2+ in the external root solution, and subsequent root uptake and compartmentation of Na in maize (Zea mays L.). In the short-term experiments (6 h), 14-d-old maize plants were exposed to various concentrations of Ca2+ at three different pH-values (6.5, 7.5, and 8.5) and two Si concentrations, i.e., low (1 mM) and high (4 mM) supply of H4SiO4. The activity of Ca2+ and Na+ in the external solution as well as the root concentrations of total and cell sap and BaCl2-exchangeble apoplastic fractions of both elements were analyzed. The pH of the nutrient solution affected neither the ion activities nor the root accumulation of both Ca2+ and Na+. At higher pH values (7.5 and 8.5) the interactions of Ca2+ and Si at high Si supply led to a decrease of Ca2+ activity and, hence, an increase of Na+ : Ca2+ activity ratio in the external root solution. Concomitantly, despite the elevated exchangeable apoplastic fraction of both Ca2+ and Na+, the total and cell sap concentrations were remarkably decreased for Ca2+ and increased for Na+ by the addition of 4 mM H4SiO4. This work demonstrates that at high Si supply extracellular Ca-Si interactions leading to lowered activity of Ca2+ might rapidly compromise the ameliorative effect of Ca2+ on Na+ accumulation in roots. Practically, Si over-fertilization of saline and, in particular, sodic soils may further promote the accumulation of Na+ in root tissues hours after Si application and, hence, increase a potential risk of Na+ toxicity.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Journal of Plant Nutrition and Soil Science",
title = "High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity",
pages = "216-210",
number = "2",
volume = "182",
doi = "10.1002/jpln.201800153"
}

Bosnić, P., Pavlicević, M., Nikolić, N.,& Nikolic, M.. (2019). High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity. in Journal of Plant Nutrition and Soil Science
Wiley-V C H Verlag Gmbh, Weinheim., 182(2), 210-216.
https://doi.org/10.1002/jpln.201800153

Bosnić P, Pavlicević M, Nikolić N, Nikolic M. High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity. in Journal of Plant Nutrition and Soil Science. 2019;182(2):210-216.
doi:10.1002/jpln.201800153 .

Bosnić, Predrag, Pavlicević, Milica, Nikolić, Nina, Nikolic, Miroslav, "High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity" in Journal of Plant Nutrition and Soil Science, 182, no. 2 (2019):210-216,
https://doi.org/10.1002/jpln.201800153 . .

TY  - JOUR
AU  - Bosnic, Dragana
AU  - Bosnić, Predrag
AU  - Nikolic, Dragana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1220
AB  - Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves
IS  - 12
VL  - 8
DO  - 10.3390/plants8120554
ER  - 

@article{
author = "Bosnic, Dragana and Bosnić, Predrag and Nikolic, Dragana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves",
number = "12",
volume = "8",
doi = "10.3390/plants8120554"
}

Bosnic, D., Bosnić, P., Nikolic, D., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel
MDPI, Basel., 8(12).
https://doi.org/10.3390/plants8120554

Bosnic D, Bosnić P, Nikolic D, Nikolic M, Samardžić JT. Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel. 2019;8(12).
doi:10.3390/plants8120554 .

Bosnic, Dragana, Bosnić, Predrag, Nikolic, Dragana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves" in Plants-Basel, 8, no. 12 (2019),
https://doi.org/10.3390/plants8120554 . .

TY  - JOUR
AU  - Nikolic, Dragana B.
AU  - Nesic, Sofija
AU  - Bosnic, Dragana
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1284
AB  - The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
VL  - 10
DO  - 10.3389/fpls.2019.00416
ER  - 

@article{
author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution",
volume = "10",
doi = "10.3389/fpls.2019.00416"
}

Nikolic, D. B., Nesic, S., Bosnic, D., Kostić Kravljanac, L., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fpls.2019.00416

Nikolic DB, Nesic S, Bosnic D, Kostić Kravljanac L, Nikolic M, Samardžić JT. Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science. 2019;10.
doi:10.3389/fpls.2019.00416 .

Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution" in Frontiers in Plant Science, 10 (2019),
https://doi.org/10.3389/fpls.2019.00416 . .

TY  - JOUR
AU  - Anicic-Urosević, Mira
AU  - Jovanović, Gordana
AU  - Stević, Nenad
AU  - Deljanin, Isidora
AU  - Nikolic, Miroslav
AU  - Tomasević, Milica
AU  - Samson, Roeland
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1214
AB  - Magnetic biomonitoring using tree leaves has been proven as a proxy for airborne particle matter (PM) pollution. Since the leaf entrapment of PM is species-specific, in this study, four tree species common in urban areas of Europe and wider (Aesculus hippocastanum, Acer platanoides, Betula pendula and Tilia cordata) were investigated to evaluate which biomonitor enables consistent 'signal' to particle and particle-bound toxic elements. The tree leaves were sampled in the central urban and suburban parks in Belgrade (Serbia) in May and September from 2011 until 2014. Magnetic PM fractions in the samples were quantified by saturation isothermal remanent magnetization (SIRM) while the concentrations of Al, Cr, Cu, Fe, Ni, Pb and Zn were determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Magnetic and elemental measurements were considered in relation to regulatory PM10 data. Median leaf SIRM values of T. cordata, A. hippocastanum and A. platanoides (174, 140 and 123 x 10(-5) x A m(2) kg(-1), respectively) implied the considerable magnetic enhancement contrary to B. pendula (68 x 10(-5) x A m(2) kg(-1)). However, B. pendula leaves showed the significant correlation between SIRM and PM10 values (r = 0.75) and SIRM and element concentrations and significant spatio-temporal differences in SIRM/element content between the studied parks/years. These results recommend B. pendula as a valuable biomonitor of PM and the associated elements. Nevertheless, both the results (high SIRM values, the significant correlation between SIRM and PM10-r = 0.71) and literature findings (abundance, adaptability, PM removal efficiency) favour A. platanoides over B. pendula in magnetic particle biomonitoring.
PB  - Springer, Dordrecht
T2  - Air Quality Atmosphere and Health
T1  - Leaves of common urban tree species (Aesculus hippocastanum, Acer platanoides, Betula pendula and Tilia cordata) as a measure of particle and particle-bound pollution: a 4-year study
EP  - 1090
IS  - 9
SP  - 1081
VL  - 12
DO  - 10.1007/s11869-019-00724-6
ER  - 

@article{
author = "Anicic-Urosević, Mira and Jovanović, Gordana and Stević, Nenad and Deljanin, Isidora and Nikolic, Miroslav and Tomasević, Milica and Samson, Roeland",
year = "2019",
abstract = "Magnetic biomonitoring using tree leaves has been proven as a proxy for airborne particle matter (PM) pollution. Since the leaf entrapment of PM is species-specific, in this study, four tree species common in urban areas of Europe and wider (Aesculus hippocastanum, Acer platanoides, Betula pendula and Tilia cordata) were investigated to evaluate which biomonitor enables consistent 'signal' to particle and particle-bound toxic elements. The tree leaves were sampled in the central urban and suburban parks in Belgrade (Serbia) in May and September from 2011 until 2014. Magnetic PM fractions in the samples were quantified by saturation isothermal remanent magnetization (SIRM) while the concentrations of Al, Cr, Cu, Fe, Ni, Pb and Zn were determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Magnetic and elemental measurements were considered in relation to regulatory PM10 data. Median leaf SIRM values of T. cordata, A. hippocastanum and A. platanoides (174, 140 and 123 x 10(-5) x A m(2) kg(-1), respectively) implied the considerable magnetic enhancement contrary to B. pendula (68 x 10(-5) x A m(2) kg(-1)). However, B. pendula leaves showed the significant correlation between SIRM and PM10 values (r = 0.75) and SIRM and element concentrations and significant spatio-temporal differences in SIRM/element content between the studied parks/years. These results recommend B. pendula as a valuable biomonitor of PM and the associated elements. Nevertheless, both the results (high SIRM values, the significant correlation between SIRM and PM10-r = 0.71) and literature findings (abundance, adaptability, PM removal efficiency) favour A. platanoides over B. pendula in magnetic particle biomonitoring.",
publisher = "Springer, Dordrecht",
journal = "Air Quality Atmosphere and Health",
title = "Leaves of common urban tree species (Aesculus hippocastanum, Acer platanoides, Betula pendula and Tilia cordata) as a measure of particle and particle-bound pollution: a 4-year study",
pages = "1090-1081",
number = "9",
volume = "12",
doi = "10.1007/s11869-019-00724-6"
}

Anicic-Urosević, M., Jovanović, G., Stević, N., Deljanin, I., Nikolic, M., Tomasević, M.,& Samson, R.. (2019). Leaves of common urban tree species (Aesculus hippocastanum, Acer platanoides, Betula pendula and Tilia cordata) as a measure of particle and particle-bound pollution: a 4-year study. in Air Quality Atmosphere and Health
Springer, Dordrecht., 12(9), 1081-1090.
https://doi.org/10.1007/s11869-019-00724-6

Anicic-Urosević M, Jovanović G, Stević N, Deljanin I, Nikolic M, Tomasević M, Samson R. Leaves of common urban tree species (Aesculus hippocastanum, Acer platanoides, Betula pendula and Tilia cordata) as a measure of particle and particle-bound pollution: a 4-year study. in Air Quality Atmosphere and Health. 2019;12(9):1081-1090.
doi:10.1007/s11869-019-00724-6 .

Anicic-Urosević, Mira, Jovanović, Gordana, Stević, Nenad, Deljanin, Isidora, Nikolic, Miroslav, Tomasević, Milica, Samson, Roeland, "Leaves of common urban tree species (Aesculus hippocastanum, Acer platanoides, Betula pendula and Tilia cordata) as a measure of particle and particle-bound pollution: a 4-year study" in Air Quality Atmosphere and Health, 12, no. 9 (2019):1081-1090,
https://doi.org/10.1007/s11869-019-00724-6 . .

TY  - JOUR
AU  - Bosnic, Dragana
AU  - Nikolic, Dragana
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Vaculik, Marek
AU  - Samardžić, Jelena T.
AU  - Nikolic, Miroslav
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1212
AB  - Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity
EP  - 641
IS  - 1-2
SP  - 629
VL  - 441
DO  - 10.1007/s11104-019-04151-5
ER  - 

@article{
author = "Bosnic, Dragana and Nikolic, Dragana and Timotijević, Gordana and Pavlović, Jelena and Vaculik, Marek and Samardžić, Jelena T. and Nikolic, Miroslav",
year = "2019",
abstract = "Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity",
pages = "641-629",
number = "1-2",
volume = "441",
doi = "10.1007/s11104-019-04151-5"
}

Bosnic, D., Nikolic, D., Timotijević, G., Pavlović, J., Vaculik, M., Samardžić, J. T.,& Nikolic, M.. (2019). Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil
Springer, Dordrecht., 441(1-2), 629-641.
https://doi.org/10.1007/s11104-019-04151-5

Bosnic D, Nikolic D, Timotijević G, Pavlović J, Vaculik M, Samardžić JT, Nikolic M. Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil. 2019;441(1-2):629-641.
doi:10.1007/s11104-019-04151-5 .

Bosnic, Dragana, Nikolic, Dragana, Timotijević, Gordana, Pavlović, Jelena, Vaculik, Marek, Samardžić, Jelena T., Nikolic, Miroslav, "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity" in Plant and Soil, 441, no. 1-2 (2019):629-641,
https://doi.org/10.1007/s11104-019-04151-5 . .

TY  - JOUR
AU  - Kolasinac, Stefan M.
AU  - Bosnić, Predrag
AU  - Lekic, Slavoljub S.
AU  - Golijan, Jelena
AU  - Petrović, Tanja
AU  - Todorović, Goran
AU  - Kostic, Aleksandar Z.
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1107
AB  - The original version of this article unfortunately contained a mistake in the author group, affiliation and funding information sections as well as the omitted acknowledgment section.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment
IS  - 11
VL  - 190
DO  - 10.1007/s10661-018-7047-y
ER  - 

@article{
author = "Kolasinac, Stefan M. and Bosnić, Predrag and Lekic, Slavoljub S. and Golijan, Jelena and Petrović, Tanja and Todorović, Goran and Kostic, Aleksandar Z.",
year = "2018",
abstract = "The original version of this article unfortunately contained a mistake in the author group, affiliation and funding information sections as well as the omitted acknowledgment section.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment",
number = "11",
volume = "190",
doi = "10.1007/s10661-018-7047-y"
}

Kolasinac, S. M., Bosnić, P., Lekic, S. S., Golijan, J., Petrović, T., Todorović, G.,& Kostic, A. Z.. (2018). Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment. in Environmental Monitoring and Assessment
Springer, Dordrecht., 190(11).
https://doi.org/10.1007/s10661-018-7047-y

Kolasinac SM, Bosnić P, Lekic SS, Golijan J, Petrović T, Todorović G, Kostic AZ. Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment. in Environmental Monitoring and Assessment. 2018;190(11).
doi:10.1007/s10661-018-7047-y .

Kolasinac, Stefan M., Bosnić, Predrag, Lekic, Slavoljub S., Golijan, Jelena, Petrović, Tanja, Todorović, Goran, Kostic, Aleksandar Z., "Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment" in Environmental Monitoring and Assessment, 190, no. 11 (2018),
https://doi.org/10.1007/s10661-018-7047-y . .

TY  - JOUR
AU  - Bosnić, Predrag
AU  - Bosnic, Dragana
AU  - Jasnic, Jovana
AU  - Nikolic, Miroslav
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1106
AB  - Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - Silicon mediates sodium transport and partitioning in maize under moderate salt stress
EP  - 687
SP  - 681
VL  - 155
DO  - 10.1016/j.envexpbot.2018.08.018
ER  - 

@article{
author = "Bosnić, Predrag and Bosnic, Dragana and Jasnic, Jovana and Nikolic, Miroslav",
year = "2018",
abstract = "Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "Silicon mediates sodium transport and partitioning in maize under moderate salt stress",
pages = "687-681",
volume = "155",
doi = "10.1016/j.envexpbot.2018.08.018"
}

Bosnić, P., Bosnic, D., Jasnic, J.,& Nikolic, M.. (2018). Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 155, 681-687.
https://doi.org/10.1016/j.envexpbot.2018.08.018

Bosnić P, Bosnic D, Jasnic J, Nikolic M. Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany. 2018;155:681-687.
doi:10.1016/j.envexpbot.2018.08.018 .

Bosnić, Predrag, Bosnic, Dragana, Jasnic, Jovana, Nikolic, Miroslav, "Silicon mediates sodium transport and partitioning in maize under moderate salt stress" in Environmental and Experimental Botany, 155 (2018):681-687,
https://doi.org/10.1016/j.envexpbot.2018.08.018 . .

TY  - JOUR
AU  - Yan, Guo-chao
AU  - Nikolic, Miroslav
AU  - Ye, Mujun
AU  - Xiao, Zhuo-xi
AU  - Liang, Yongchao
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1174
AB  - Although silicon (Si) is ubiquitous in soil and plant, evidence is still lacking that Si is essential for higher plants. However, it has been well documented that Si is beneficial for healthy growth of many plant species. Si can promote plant mechanical strength, light interception, as well as resistance to various forms of abiotic and biotic stress, thus improving both yield and quality. Indeed, application of Si fertilizer is a rather common agricultural practice in many countries and regions. As the beneficial effects provided by Si are closely correlated with Si accumulation level in plant, elucidating the possible mechanisms of Si uptake and transport in plants is extremely important to utilize the Si-induced beneficial effects in plants. Recently, rapid progress has been made in unveiling molecular mechanisms of Si uptake and transport in plants. Based on the cooperation of Si influx channels and efflux transporters, a model to decipher Si uptake, transport and distribution system in higher plants has been developed, which involves uptake and radial transport in root, xylem and inter-vascular transport and xylem unloading and deposition in leaf. In this paper, we overviewed the updated knowledge concerning Si uptake, transport and accumulation and its significance for the major crops of agricultural importance and highlighted the further research needs as well.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Integrative Agriculture
T1  - Silicon acquisition and accumulation in plant and its significance for agriculture
EP  - 2150
IS  - 10
SP  - 2138
VL  - 17
DO  - 10.1016/S2095-3119(18)62037-4
ER  - 

@article{
author = "Yan, Guo-chao and Nikolic, Miroslav and Ye, Mujun and Xiao, Zhuo-xi and Liang, Yongchao",
year = "2018",
abstract = "Although silicon (Si) is ubiquitous in soil and plant, evidence is still lacking that Si is essential for higher plants. However, it has been well documented that Si is beneficial for healthy growth of many plant species. Si can promote plant mechanical strength, light interception, as well as resistance to various forms of abiotic and biotic stress, thus improving both yield and quality. Indeed, application of Si fertilizer is a rather common agricultural practice in many countries and regions. As the beneficial effects provided by Si are closely correlated with Si accumulation level in plant, elucidating the possible mechanisms of Si uptake and transport in plants is extremely important to utilize the Si-induced beneficial effects in plants. Recently, rapid progress has been made in unveiling molecular mechanisms of Si uptake and transport in plants. Based on the cooperation of Si influx channels and efflux transporters, a model to decipher Si uptake, transport and distribution system in higher plants has been developed, which involves uptake and radial transport in root, xylem and inter-vascular transport and xylem unloading and deposition in leaf. In this paper, we overviewed the updated knowledge concerning Si uptake, transport and accumulation and its significance for the major crops of agricultural importance and highlighted the further research needs as well.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Integrative Agriculture",
title = "Silicon acquisition and accumulation in plant and its significance for agriculture",
pages = "2150-2138",
number = "10",
volume = "17",
doi = "10.1016/S2095-3119(18)62037-4"
}

Yan, G., Nikolic, M., Ye, M., Xiao, Z.,& Liang, Y.. (2018). Silicon acquisition and accumulation in plant and its significance for agriculture. in Journal of Integrative Agriculture
Elsevier Sci Ltd, Oxford., 17(10), 2138-2150.
https://doi.org/10.1016/S2095-3119(18)62037-4

Yan G, Nikolic M, Ye M, Xiao Z, Liang Y. Silicon acquisition and accumulation in plant and its significance for agriculture. in Journal of Integrative Agriculture. 2018;17(10):2138-2150.
doi:10.1016/S2095-3119(18)62037-4 .

Yan, Guo-chao, Nikolic, Miroslav, Ye, Mujun, Xiao, Zhuo-xi, Liang, Yongchao, "Silicon acquisition and accumulation in plant and its significance for agriculture" in Journal of Integrative Agriculture, 17, no. 10 (2018):2138-2150,
https://doi.org/10.1016/S2095-3119(18)62037-4 . .

TY  - JOUR
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1172
AB  - Long-term potentials and constraints of nature to spontaneously recover after severe degradation by toxic mine waste, and general principles of mutual modifications of spontaneous vegetation and soils during this process, have remained widely unknown. This study investigates the effect of flooding on natural restoration of a model locality in Eastern Serbia, complexly degraded by 70-years fluvial deposition of sulfidic copper (Cu) mine tailings in a floodplain along 80km of the polluted river flow. We analyzed multivariate response of forest vegetation (floristic and structural parameters) together with physical and chemical properties of concomitant soils and sediments. Floods strongly modify the interactions between soil and vegetation. Three very different types of forest vegetation constitute the response of the nature to key soil adverse factors (excessive Cu availability, low nutrients, and low pH); combined with drought, these constraints completely inhibit revegetation. Continual flooding after mine closure, despite the pollution it still brings in, fosters a faster development of highly specialized vegetation and most importantly faster buildup of soil organic matter necessary for phytostabilization of substantial amounts of Cu tailings present in the floodplain. We show that enhanced nutrient fluxes facilitated by natural flooding regime might overrun the constraining effect of deposited Cu also for natural vegetation.
PB  - Wiley, Hoboken
T2  - Land Degradation & Development
T1  - To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings
EP  - 1924
IS  - 6
SP  - 1915
VL  - 29
DO  - 10.1002/ldr.2921
ER  - 

@article{
author = "Nikolić, Nina and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav",
year = "2018",
abstract = "Long-term potentials and constraints of nature to spontaneously recover after severe degradation by toxic mine waste, and general principles of mutual modifications of spontaneous vegetation and soils during this process, have remained widely unknown. This study investigates the effect of flooding on natural restoration of a model locality in Eastern Serbia, complexly degraded by 70-years fluvial deposition of sulfidic copper (Cu) mine tailings in a floodplain along 80km of the polluted river flow. We analyzed multivariate response of forest vegetation (floristic and structural parameters) together with physical and chemical properties of concomitant soils and sediments. Floods strongly modify the interactions between soil and vegetation. Three very different types of forest vegetation constitute the response of the nature to key soil adverse factors (excessive Cu availability, low nutrients, and low pH); combined with drought, these constraints completely inhibit revegetation. Continual flooding after mine closure, despite the pollution it still brings in, fosters a faster development of highly specialized vegetation and most importantly faster buildup of soil organic matter necessary for phytostabilization of substantial amounts of Cu tailings present in the floodplain. We show that enhanced nutrient fluxes facilitated by natural flooding regime might overrun the constraining effect of deposited Cu also for natural vegetation.",
publisher = "Wiley, Hoboken",
journal = "Land Degradation & Development",
title = "To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings",
pages = "1924-1915",
number = "6",
volume = "29",
doi = "10.1002/ldr.2921"
}

Nikolić, N., Kostić Kravljanac, L.,& Nikolic, M.. (2018). To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings. in Land Degradation & Development
Wiley, Hoboken., 29(6), 1915-1924.
https://doi.org/10.1002/ldr.2921

Nikolić N, Kostić Kravljanac L, Nikolic M. To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings. in Land Degradation & Development. 2018;29(6):1915-1924.
doi:10.1002/ldr.2921 .

Nikolić, Nina, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, "To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings" in Land Degradation & Development, 29, no. 6 (2018):1915-1924,
https://doi.org/10.1002/ldr.2921 . .

TY  - JOUR
AU  - Ribera-Fonseca, Alejandra
AU  - Rumpel, Cornelia
AU  - de, la Luz Mora, Maria
AU  - Nikolic, Miroslav
AU  - Cartes, Paula
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1147
AB  - Evidence indicates that silicon (Si) alleviates diverse stresses by improving the antioxidant capacity and phenolics metabolism of plants. We assessed the effect of sodium silicate (Na2SiO3) and calcium silicate (CaSiO3) on Si and aluminium (Al) uptake, antioxidant performance and phenolics (with antioxidant or structural function) of ryegrass cultivated on an acid Andisol under greenhouse conditions. Ryegrass was treated with either sodium silicate or calcium silicate at gradually increasing doses (250, 500 and 1000mg Si kg(-1) soil). Yield and concentrations of Si and Al were measured in roots and two shoot cuts. At the first cut, phenols, antioxidant enzymes, antioxidant capacity, lipid peroxidation and lignin production and composition were also determined. Ryegrass supplied with sodium silicate exhibited the highest Si content. Root Si was closely correlated with Al or Si:Al ratio. Shoot Si uptake increased total phenols and activities of antioxidant enzymes (CAT, APX and POD), but reduced lipid peroxidation. Silicon also changed the lignin production and composition in shoots at the highest sodium silicate dose. Silicon uptake reduced the deleterious effect of soil acidity in ryegrass. Sodium silicate had the greatest influence on the antioxidant system through enhancement of phenols production and antioxidant enzyme activation. Peroxidase activity appears to be associated with increased lignin biosynthesis in plants supplied with sodium silicate.
PB  - Csiro Publishing, Clayton
T2  - Crop & Pasture Science
T1  - Sodium silicate and calcium silicate differentially affect silicon and aluminium uptake, antioxidant performance and phenolics metabolism of ryegrass in an acid Andisol
EP  - 215
IS  - 2
SP  - 205
VL  - 69
DO  - 10.1071/CP17202
ER  - 

@article{
author = "Ribera-Fonseca, Alejandra and Rumpel, Cornelia and de, la Luz Mora, Maria and Nikolic, Miroslav and Cartes, Paula",
year = "2018",
abstract = "Evidence indicates that silicon (Si) alleviates diverse stresses by improving the antioxidant capacity and phenolics metabolism of plants. We assessed the effect of sodium silicate (Na2SiO3) and calcium silicate (CaSiO3) on Si and aluminium (Al) uptake, antioxidant performance and phenolics (with antioxidant or structural function) of ryegrass cultivated on an acid Andisol under greenhouse conditions. Ryegrass was treated with either sodium silicate or calcium silicate at gradually increasing doses (250, 500 and 1000mg Si kg(-1) soil). Yield and concentrations of Si and Al were measured in roots and two shoot cuts. At the first cut, phenols, antioxidant enzymes, antioxidant capacity, lipid peroxidation and lignin production and composition were also determined. Ryegrass supplied with sodium silicate exhibited the highest Si content. Root Si was closely correlated with Al or Si:Al ratio. Shoot Si uptake increased total phenols and activities of antioxidant enzymes (CAT, APX and POD), but reduced lipid peroxidation. Silicon also changed the lignin production and composition in shoots at the highest sodium silicate dose. Silicon uptake reduced the deleterious effect of soil acidity in ryegrass. Sodium silicate had the greatest influence on the antioxidant system through enhancement of phenols production and antioxidant enzyme activation. Peroxidase activity appears to be associated with increased lignin biosynthesis in plants supplied with sodium silicate.",
publisher = "Csiro Publishing, Clayton",
journal = "Crop & Pasture Science",
title = "Sodium silicate and calcium silicate differentially affect silicon and aluminium uptake, antioxidant performance and phenolics metabolism of ryegrass in an acid Andisol",
pages = "215-205",
number = "2",
volume = "69",
doi = "10.1071/CP17202"
}

Ribera-Fonseca, A., Rumpel, C., de, l. L. M. M., Nikolic, M.,& Cartes, P.. (2018). Sodium silicate and calcium silicate differentially affect silicon and aluminium uptake, antioxidant performance and phenolics metabolism of ryegrass in an acid Andisol. in Crop & Pasture Science
Csiro Publishing, Clayton., 69(2), 205-215.
https://doi.org/10.1071/CP17202

Ribera-Fonseca A, Rumpel C, de LLMM, Nikolic M, Cartes P. Sodium silicate and calcium silicate differentially affect silicon and aluminium uptake, antioxidant performance and phenolics metabolism of ryegrass in an acid Andisol. in Crop & Pasture Science. 2018;69(2):205-215.
doi:10.1071/CP17202 .

Ribera-Fonseca, Alejandra, Rumpel, Cornelia, de, la Luz Mora, Maria, Nikolic, Miroslav, Cartes, Paula, "Sodium silicate and calcium silicate differentially affect silicon and aluminium uptake, antioxidant performance and phenolics metabolism of ryegrass in an acid Andisol" in Crop & Pasture Science, 69, no. 2 (2018):205-215,
https://doi.org/10.1071/CP17202 . .

TY  - JOUR
AU  - Savić, J.
AU  - Stević, N.
AU  - Maksimović, Vuk
AU  - Samardžić, Jelena T.
AU  - Nikolic, D.B.
AU  - Nikolic, M.
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1179
AB  - Aluminium (Al) toxicity in acid soils is a global problem. Here, we investigated Al tolerance in high yielding winter wheat (Triticum aestivum L.) cultivars bred in Serbia. The common relative mot length (RRL) test for Al tolerance, and both physiological (malate efflux) and molecular (Aluminium-Activated Malate Transporter 1 [TaALMT1] expression) approaches were used for this characterization. Both moderately Al-tolerant cvs. Ljiljana and Arabeska showed significantly higher malate efflux rate from the root tips in comparison to moderately Al-sensitive cv. Pobeda and followed the RRL pattern. Irrespectively of Al supply, moderately Al-tolerant cultivars showed significantly higher relative TaALMT1 expression than the Al-sensitive ones. A considerably high level of Al tolerance was found in cv. Ljiljana, which showed the highest Al-induced malate efflux along with the highest constitutive expression level of TaALMT1 transcripts. Our results also demonstrate that Al tolerance is based on a constitutive trait of high TaALMT1 expression and malate efflux in wheat roots, resulting in a decrease in root length reduction.
PB  - Sociedad Chilena de la Ciencia del Suelo
T2  - Journal of Soil Science and Plant Nutrition
T1  - Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance
EP  - 99
IS  - 1
SP  - 90
VL  - 18
DO  - 10.4067/S0718-95162018005000402
ER  - 

@article{
author = "Savić, J. and Stević, N. and Maksimović, Vuk and Samardžić, Jelena T. and Nikolic, D.B. and Nikolic, M.",
year = "2018",
abstract = "Aluminium (Al) toxicity in acid soils is a global problem. Here, we investigated Al tolerance in high yielding winter wheat (Triticum aestivum L.) cultivars bred in Serbia. The common relative mot length (RRL) test for Al tolerance, and both physiological (malate efflux) and molecular (Aluminium-Activated Malate Transporter 1 [TaALMT1] expression) approaches were used for this characterization. Both moderately Al-tolerant cvs. Ljiljana and Arabeska showed significantly higher malate efflux rate from the root tips in comparison to moderately Al-sensitive cv. Pobeda and followed the RRL pattern. Irrespectively of Al supply, moderately Al-tolerant cultivars showed significantly higher relative TaALMT1 expression than the Al-sensitive ones. A considerably high level of Al tolerance was found in cv. Ljiljana, which showed the highest Al-induced malate efflux along with the highest constitutive expression level of TaALMT1 transcripts. Our results also demonstrate that Al tolerance is based on a constitutive trait of high TaALMT1 expression and malate efflux in wheat roots, resulting in a decrease in root length reduction.",
publisher = "Sociedad Chilena de la Ciencia del Suelo",
journal = "Journal of Soil Science and Plant Nutrition",
title = "Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance",
pages = "99-90",
number = "1",
volume = "18",
doi = "10.4067/S0718-95162018005000402"
}

Savić, J., Stević, N., Maksimović, V., Samardžić, J. T., Nikolic, D.B.,& Nikolic, M.. (2018). Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance. in Journal of Soil Science and Plant Nutrition
Sociedad Chilena de la Ciencia del Suelo., 18(1), 90-99.
https://doi.org/10.4067/S0718-95162018005000402

Savić J, Stević N, Maksimović V, Samardžić JT, Nikolic D, Nikolic M. Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance. in Journal of Soil Science and Plant Nutrition. 2018;18(1):90-99.
doi:10.4067/S0718-95162018005000402 .

Savić, J., Stević, N., Maksimović, Vuk, Samardžić, Jelena T., Nikolic, D.B., Nikolic, M., "Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance" in Journal of Soil Science and Plant Nutrition, 18, no. 1 (2018):90-99,
https://doi.org/10.4067/S0718-95162018005000402 . .

TY  - CONF
AU  - Nikolic, Miroslav
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2149
AB  - Silicon is the only known mineral element that effectively alleviates multiple environmental stress in many plant species. Over the past decade rapid progress has been made in understanding the mechanisms through which Si mediates mineral excess and/or toxicity stress. It has been demonstrated that Si mediates uptake and transport of mineral elements at excess by regulating expression of various transporter genes (e.g. Kim et al., 2014; Akcay and Erkan, 2016; Che et al., 2016); however, the role of Si in nutrient uptake and transport under nutrient deficiency conditions is still insufficiently understood. In this presentation, I will talk about Si influence on (a) root P- starvation responses for rhizosphere mobilization and uptake of Pi in wheat (Triticum aestivum) and (b) acquisition and long-distance transport of Fe in cucumber (Cucumis sativus) under low Fe conditions; our recent unpublished work on (c) Si-regulated expression of the transporters involved in Na homeostasis in maize (Zea mays) subjected to NaCl stress will also be discussed.
PB  - University of Copenhagen, Denmark
C3  - XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark
T1  - Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2149
ER  - 

@conference{
author = "Nikolic, Miroslav and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag",
year = "2017",
abstract = "Silicon is the only known mineral element that effectively alleviates multiple environmental stress in many plant species. Over the past decade rapid progress has been made in understanding the mechanisms through which Si mediates mineral excess and/or toxicity stress. It has been demonstrated that Si mediates uptake and transport of mineral elements at excess by regulating expression of various transporter genes (e.g. Kim et al., 2014; Akcay and Erkan, 2016; Che et al., 2016); however, the role of Si in nutrient uptake and transport under nutrient deficiency conditions is still insufficiently understood. In this presentation, I will talk about Si influence on (a) root P- starvation responses for rhizosphere mobilization and uptake of Pi in wheat (Triticum aestivum) and (b) acquisition and long-distance transport of Fe in cucumber (Cucumis sativus) under low Fe conditions; our recent unpublished work on (c) Si-regulated expression of the transporters involved in Na homeostasis in maize (Zea mays) subjected to NaCl stress will also be discussed.",
publisher = "University of Copenhagen, Denmark",
journal = "XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark",
title = "Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2149"
}

Nikolic, M., Kostić Kravljanac, L., Pavlović, J.,& Bosnić, P.. (2017). Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress. in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark
University of Copenhagen, Denmark..
https://hdl.handle.net/21.15107/rcub_rimsi_2149

Nikolic M, Kostić Kravljanac L, Pavlović J, Bosnić P. Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress. in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark. 2017;.
https://hdl.handle.net/21.15107/rcub_rimsi_2149 .

Nikolic, Miroslav, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, "Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress" in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark (2017),
https://hdl.handle.net/21.15107/rcub_rimsi_2149 .

TY  - THES
AU  - Pavlović, Jelena
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5140
UR  - https://nardus.mpn.gov.rs/handle/123456789/8430
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:15995/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49212431
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/16
AB  - Nedostatak gvožđa (Fe) predstavlja jedan od glavnih ograničavajućih faktora u proizvodnji useva širom sveta, koji značajno umanjuje prinos i kvalitet. Sa druge strane, koristan efekat silicijuma (Si) na rastenje i razviće biljaka, posebno u uslovima stresa, dobro je poznat u literaturi. Cilj ove teze je bio da se prouči mehanizam kako Si ublažava stres izazvan nedostatkom Fe kod dikotiledonih biljaka (strategija I usvajanja Fe), o kome se do sada ništa nije znalo. Kao model biljka odabran je krastavac (Cucumis sativus L.) kao vrsta koja akumulira Si. Izvršena su detaljna izučavanja mobilnosti Fe u apoplastu, biosinteze jedinjenja koja mobilišu Fe i njegovog daljeg usvajanja na bazi redukcije u korenu, zajedno sa ekspresijom gena uključenih u te procese. U nadzemnom delu biljke, proučavana je remobilizacija Fe iz starijih u mlađe listove, tako što su paralelno mereni distribucija 59Fe u listovima na različitim pozicijama i koncentracija helatora nikocijanamina (NA), zajedno sa ekspresijom gena za nikocijanamin sintazu (NAS) uključenu u njegovu biosintezu i YSL (eng. yellow stripe-like) transporter koji posreduju u transportu Fe-NA floemom. Ishrana Si uticala je na povećanu akumulaciju Fe u apoplastu i Fe-mobilizirajućih jedinjenja u korenu, kao i na stimulaciju mašinerije za usvajanje Fe. U listovima, Si je uticao na relativnu distribuciju Fe povećavajući remobilizaciju Fe iz starijih listova zbog povećane akumulacije NA i ekspresije YSL1, što je pospešilo heliranje Fe i njegovu retranslokaciju u mlađe listove. Ova teza pruža, po prvi put, objašnjenje mehanizma koji Si ispoljava u prevazilaženju nedostatka Fe, a koji se sastoji u povećanju rezervi Fe u apoplastu korena, njegove mobilizacije i trasporta iz korenu u izdanak, kao i poboljšane remobilizacije iz starijih u mlađe listove.
AB  - Iron (Fe) deficiency represents a major limiting factor for crop production worldwide, affecting both crop yield and quality. Beneficial effect of silicon (Si) on plant growth and development, especially under stress conditions, is well documented in the literature. The objective of this thesis was to investigate the mechanisms of how Si ameliorates Fe deficiency in Strategy I plants, which has not been understood until recently. Cucumber (Cucumis sativus L.) was selected as a common model plant for Fe research and which is also known as a Si-accumulating dicot. Detailed analyzes of the dynamics of apoplastic Fe, biosynthesis of Fe-mobilizing compounds and reduction-based Fe acquisition were performed in roots along with the expression of relevant genes involved in these processes. At the shoot level, distribution of 59Fe was measured in the in leaves at different positions and developmental stages in parallel with the concentration of the Fe chelator nicotianamine (NA) and the gene expression of nicotianamine synthase (NAS) involved in its biosynthesis. The expression of yellow stripe-like (YSL) transporters mediating pholem transport of Fe-NA in shoot was also determined. Silicon nutrition increased the accumulation of apoplastic Fe and Fe mobilizing compounds in roots. In leaves, Si affected relative Fe distribution by enhancing Fe remobilization from old leaves via increased NA accumulation and expression of the YSL1, which stimulated Fe chelation and its retranslocation to younger leaves. This thesis for the first time elucidates the mechanism of Si-mediated alleviation of Fe deficiency stress, by increasing the root apoplastic Fe pool, enhancing its acquisition by roots and translocation to shoots and also remobilization of Fe from older to younger leaves.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.)
T1  - The Role of Silicon in Alleviation of Iron Deficiency in Cucumber (Cucumis sativus L.)
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8430
ER  - 

@phdthesis{
author = "Pavlović, Jelena",
year = "2017",
abstract = "Nedostatak gvožđa (Fe) predstavlja jedan od glavnih ograničavajućih faktora u proizvodnji useva širom sveta, koji značajno umanjuje prinos i kvalitet. Sa druge strane, koristan efekat silicijuma (Si) na rastenje i razviće biljaka, posebno u uslovima stresa, dobro je poznat u literaturi. Cilj ove teze je bio da se prouči mehanizam kako Si ublažava stres izazvan nedostatkom Fe kod dikotiledonih biljaka (strategija I usvajanja Fe), o kome se do sada ništa nije znalo. Kao model biljka odabran je krastavac (Cucumis sativus L.) kao vrsta koja akumulira Si. Izvršena su detaljna izučavanja mobilnosti Fe u apoplastu, biosinteze jedinjenja koja mobilišu Fe i njegovog daljeg usvajanja na bazi redukcije u korenu, zajedno sa ekspresijom gena uključenih u te procese. U nadzemnom delu biljke, proučavana je remobilizacija Fe iz starijih u mlađe listove, tako što su paralelno mereni distribucija 59Fe u listovima na različitim pozicijama i koncentracija helatora nikocijanamina (NA), zajedno sa ekspresijom gena za nikocijanamin sintazu (NAS) uključenu u njegovu biosintezu i YSL (eng. yellow stripe-like) transporter koji posreduju u transportu Fe-NA floemom. Ishrana Si uticala je na povećanu akumulaciju Fe u apoplastu i Fe-mobilizirajućih jedinjenja u korenu, kao i na stimulaciju mašinerije za usvajanje Fe. U listovima, Si je uticao na relativnu distribuciju Fe povećavajući remobilizaciju Fe iz starijih listova zbog povećane akumulacije NA i ekspresije YSL1, što je pospešilo heliranje Fe i njegovu retranslokaciju u mlađe listove. Ova teza pruža, po prvi put, objašnjenje mehanizma koji Si ispoljava u prevazilaženju nedostatka Fe, a koji se sastoji u povećanju rezervi Fe u apoplastu korena, njegove mobilizacije i trasporta iz korenu u izdanak, kao i poboljšane remobilizacije iz starijih u mlađe listove., Iron (Fe) deficiency represents a major limiting factor for crop production worldwide, affecting both crop yield and quality. Beneficial effect of silicon (Si) on plant growth and development, especially under stress conditions, is well documented in the literature. The objective of this thesis was to investigate the mechanisms of how Si ameliorates Fe deficiency in Strategy I plants, which has not been understood until recently. Cucumber (Cucumis sativus L.) was selected as a common model plant for Fe research and which is also known as a Si-accumulating dicot. Detailed analyzes of the dynamics of apoplastic Fe, biosynthesis of Fe-mobilizing compounds and reduction-based Fe acquisition were performed in roots along with the expression of relevant genes involved in these processes. At the shoot level, distribution of 59Fe was measured in the in leaves at different positions and developmental stages in parallel with the concentration of the Fe chelator nicotianamine (NA) and the gene expression of nicotianamine synthase (NAS) involved in its biosynthesis. The expression of yellow stripe-like (YSL) transporters mediating pholem transport of Fe-NA in shoot was also determined. Silicon nutrition increased the accumulation of apoplastic Fe and Fe mobilizing compounds in roots. In leaves, Si affected relative Fe distribution by enhancing Fe remobilization from old leaves via increased NA accumulation and expression of the YSL1, which stimulated Fe chelation and its retranslocation to younger leaves. This thesis for the first time elucidates the mechanism of Si-mediated alleviation of Fe deficiency stress, by increasing the root apoplastic Fe pool, enhancing its acquisition by roots and translocation to shoots and also remobilization of Fe from older to younger leaves.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.), The Role of Silicon in Alleviation of Iron Deficiency in Cucumber (Cucumis sativus L.)",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8430"
}

Pavlović, J.. (2017). Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.). 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_8430

Pavlović J. Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.). 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_8430 .

Pavlović, Jelena, "Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.)" (2017),
https://hdl.handle.net/21.15107/rcub_nardus_8430 .

TY  - JOUR
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Bosnic, Dragana
AU  - Samardžić, Jelena T.
AU  - Nikolic, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1067
AB  - Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions
EP  - 455
IS  - 1-2
SP  - 447
VL  - 419
DO  - 10.1007/s11104-017-3364-0
ER  - 

@article{
author = "Kostić Kravljanac, Ljiljana and Nikolić, Nina and Bosnic, Dragana and Samardžić, Jelena T. and Nikolic, Miroslav",
year = "2017",
abstract = "Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions",
pages = "455-447",
number = "1-2",
volume = "419",
doi = "10.1007/s11104-017-3364-0"
}

Kostić Kravljanac, L., Nikolić, N., Bosnic, D., Samardžić, J. T.,& Nikolic, M.. (2017). Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil
Springer, Dordrecht., 419(1-2), 447-455.
https://doi.org/10.1007/s11104-017-3364-0

Kostić Kravljanac L, Nikolić N, Bosnic D, Samardžić JT, Nikolic M. Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil. 2017;419(1-2):447-455.
doi:10.1007/s11104-017-3364-0 .

Kostić Kravljanac, Ljiljana, Nikolić, Nina, Bosnic, Dragana, Samardžić, Jelena T., Nikolic, Miroslav, "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions" in Plant and Soil, 419, no. 1-2 (2017):447-455,
https://doi.org/10.1007/s11104-017-3364-0 . .

TY  - JOUR
AU  - Nikolic, Miroslav
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
AU  - Stević, Nenad
AU  - Savić, Jasna
AU  - Hristov, Nikola
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/949
AB  - The deficiency of zinc (Zn) and iron (Fe) is a global issue causing not only considerable yield losses of food crops but also serious health problems. We have analysed Zn and Fe concentrations in the grains of two bread wheat cultivars along native gradient of micronutrient availability throughout Serbia. Although only 13% of the soil samples were Zn deficient and none was Fe deficient, the levels of these micronutrients in grain were rather low (median values of 21 mg kg(-1) for Zn and 36 mg kg(-1) for Fe), and even less adequate in white flour. Moreover, excessive P fertilization of calcareous soils in the major wheat growing areas strongly correlated with lower grain concentration of Zn. Our results imply that a latent Zn deficiency in wheat grain poses a high risk for grain quality relevant to human health in Serbia, where wheat bread is a staple food. k.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition
EP  - 148
SP  - 141
VL  - 553
DO  - 10.1016/j.scitotenv.2016.02.102
ER  - 

@article{
author = "Nikolic, Miroslav and Nikolić, Nina and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag and Stević, Nenad and Savić, Jasna and Hristov, Nikola",
year = "2016",
abstract = "The deficiency of zinc (Zn) and iron (Fe) is a global issue causing not only considerable yield losses of food crops but also serious health problems. We have analysed Zn and Fe concentrations in the grains of two bread wheat cultivars along native gradient of micronutrient availability throughout Serbia. Although only 13% of the soil samples were Zn deficient and none was Fe deficient, the levels of these micronutrients in grain were rather low (median values of 21 mg kg(-1) for Zn and 36 mg kg(-1) for Fe), and even less adequate in white flour. Moreover, excessive P fertilization of calcareous soils in the major wheat growing areas strongly correlated with lower grain concentration of Zn. Our results imply that a latent Zn deficiency in wheat grain poses a high risk for grain quality relevant to human health in Serbia, where wheat bread is a staple food. k.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition",
pages = "148-141",
volume = "553",
doi = "10.1016/j.scitotenv.2016.02.102"
}

Nikolic, M., Nikolić, N., Kostić Kravljanac, L., Pavlović, J., Bosnić, P., Stević, N., Savić, J.,& Hristov, N.. (2016). The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition. in Science of the Total Environment
Elsevier, Amsterdam., 553, 141-148.
https://doi.org/10.1016/j.scitotenv.2016.02.102

Nikolic M, Nikolić N, Kostić Kravljanac L, Pavlović J, Bosnić P, Stević N, Savić J, Hristov N. The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition. in Science of the Total Environment. 2016;553:141-148.
doi:10.1016/j.scitotenv.2016.02.102 .

Nikolic, Miroslav, Nikolić, Nina, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, Stević, Nenad, Savić, Jasna, Hristov, Nikola, "The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition" in Science of the Total Environment, 553 (2016):141-148,
https://doi.org/10.1016/j.scitotenv.2016.02.102 . .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Samardžić, Jelena T.
AU  - Kostić Kravljanac, Ljiljana
AU  - Laursen, Kristian H.
AU  - Natic, Maja
AU  - Timotijević, Gordana
AU  - Schjoerring, Jan K.
AU  - Nikolic, Miroslav
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1002
AB  - Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.
PB  - Oxford Univ Press, Oxford
T2  - Annals of Botany
T1  - Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions
EP  - 280
IS  - 2
SP  - 271
VL  - 118
DO  - 10.1093/aob/mcw105
ER  - 

@article{
author = "Pavlović, Jelena and Samardžić, Jelena T. and Kostić Kravljanac, Ljiljana and Laursen, Kristian H. and Natic, Maja and Timotijević, Gordana and Schjoerring, Jan K. and Nikolic, Miroslav",
year = "2016",
abstract = "Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.",
publisher = "Oxford Univ Press, Oxford",
journal = "Annals of Botany",
title = "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions",
pages = "280-271",
number = "2",
volume = "118",
doi = "10.1093/aob/mcw105"
}

Pavlović, J., Samardžić, J. T., Kostić Kravljanac, L., Laursen, K. H., Natic, M., Timotijević, G., Schjoerring, J. K.,& Nikolic, M.. (2016). Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany
Oxford Univ Press, Oxford., 118(2), 271-280.
https://doi.org/10.1093/aob/mcw105

Pavlović J, Samardžić JT, Kostić Kravljanac L, Laursen KH, Natic M, Timotijević G, Schjoerring JK, Nikolic M. Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany. 2016;118(2):271-280.
doi:10.1093/aob/mcw105 .

Pavlović, Jelena, Samardžić, Jelena T., Kostić Kravljanac, Ljiljana, Laursen, Kristian H., Natic, Maja, Timotijević, Gordana, Schjoerring, Jan K., Nikolic, Miroslav, "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions" in Annals of Botany, 118, no. 2 (2016):271-280,
https://doi.org/10.1093/aob/mcw105 . .

TY  - JOUR
AU  - Stević, Nenad
AU  - Korać Jačić, Jelena
AU  - Pavlović, Jelena
AU  - Nikolic, Miroslav
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1008
AB  - The supplementation of monosilicic acid [Si(OH)(4)] to the root growing medium is known to protect plants from toxic levels of iron (Fe), copper (Cu) and manganese (Mn), but also to mitigate deficiency of Fe and Mn. However, the physicochemical bases of these alleviating mechanisms are not fully understood. Here we applied low-T electron paramagnetic resonance (EPR) spectroscopy to examine the formation of complexes of Si(OH)(4) with Mn2+, Fe3+, and Cu2+ in water and in xylem sap of cucumber (Cucumis sativus L.) grown without or with supply of Si(OH)(4). EPR, which is also useful in establishing the redox state of these metals, was combined with measurements of total concentrations of metals in xylem sap by inductive coupled plasma. Our results show that Si(OH)(4) forms coordination bonds with all three metals. The strongest interactions of Si(OH)(4) appear to be with Cu2+ (1/1 stoichiometry) which might lead to Cu precipitation. In line with this in vitro findings, Si(OH)(4) supply to cucumber resulted in dramatically lower concentration of this metal in the xylem sap. Further, it was demonstrated that Si(OH)(4) supplementation causes pro-reductive changes that contribute to the maintenance of Fe and, in particular, Mn in the xylem sap in bioavailable 2+ form. Our results shed more light on the intertwined reactions between Si(OH)(4) and transition metals in plant fluids (e.g. xylem sap).
PB  - Springer, Dordrecht
T2  - Biometals
T1  - Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study
EP  - 951
IS  - 5
SP  - 945
VL  - 29
DO  - 10.1007/s10534-016-9966-9
ER  - 

@article{
author = "Stević, Nenad and Korać Jačić, Jelena and Pavlović, Jelena and Nikolic, Miroslav",
year = "2016",
abstract = "The supplementation of monosilicic acid [Si(OH)(4)] to the root growing medium is known to protect plants from toxic levels of iron (Fe), copper (Cu) and manganese (Mn), but also to mitigate deficiency of Fe and Mn. However, the physicochemical bases of these alleviating mechanisms are not fully understood. Here we applied low-T electron paramagnetic resonance (EPR) spectroscopy to examine the formation of complexes of Si(OH)(4) with Mn2+, Fe3+, and Cu2+ in water and in xylem sap of cucumber (Cucumis sativus L.) grown without or with supply of Si(OH)(4). EPR, which is also useful in establishing the redox state of these metals, was combined with measurements of total concentrations of metals in xylem sap by inductive coupled plasma. Our results show that Si(OH)(4) forms coordination bonds with all three metals. The strongest interactions of Si(OH)(4) appear to be with Cu2+ (1/1 stoichiometry) which might lead to Cu precipitation. In line with this in vitro findings, Si(OH)(4) supply to cucumber resulted in dramatically lower concentration of this metal in the xylem sap. Further, it was demonstrated that Si(OH)(4) supplementation causes pro-reductive changes that contribute to the maintenance of Fe and, in particular, Mn in the xylem sap in bioavailable 2+ form. Our results shed more light on the intertwined reactions between Si(OH)(4) and transition metals in plant fluids (e.g. xylem sap).",
publisher = "Springer, Dordrecht",
journal = "Biometals",
title = "Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study",
pages = "951-945",
number = "5",
volume = "29",
doi = "10.1007/s10534-016-9966-9"
}

Stević, N., Korać Jačić, J., Pavlović, J.,& Nikolic, M.. (2016). Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study. in Biometals
Springer, Dordrecht., 29(5), 945-951.
https://doi.org/10.1007/s10534-016-9966-9

Stević N, Korać Jačić J, Pavlović J, Nikolic M. Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study. in Biometals. 2016;29(5):945-951.
doi:10.1007/s10534-016-9966-9 .

Stević, Nenad, Korać Jačić, Jelena, Pavlović, Jelena, Nikolic, Miroslav, "Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study" in Biometals, 29, no. 5 (2016):945-951,
https://doi.org/10.1007/s10534-016-9966-9 . .

TY  - JOUR
AU  - Nikolić, Nina
AU  - Boecker, Reinhard
AU  - Nikolic, Miroslav
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/976
AB  - Despite the growing popularity of ecological restoration approach, data on primary succession on toxic post-mining substrates, under site environmental conditions which considerably differ from the surrounding environment, are still scarce. Here, we studied the spontaneous vegetation development on an unusual locality created by long-term and large-scale fluvial deposition of sulphidic tailings from a copper mine in a pronouncedly xerothermic, calcareous surrounding. We performed multivariate analyses of soil samples (20 physical and chemical parameters) and vegetation samples (floristic and structural parameters in three types of occurring forests), collected along the pollution gradients throughout the affected floodplain. The nature can cope with two types of imposed constraints: (a) excessive Cu concentrations and (b) very low pH, combined with nutrient deficiency. The former will still allow convergence to the original vegetation, while the latter will result in novel, depauperate assemblages of species typical for cooler and moister climate. Our results for the first time demonstrate that with the increasing severity of environmental filtering, the relative importance of the surrounding vegetation for primary succession strongly decreases.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Long-term passive restoration following fluvial deposition of sulphidic copper tailings: nature filters out the solutions
EP  - 13680
IS  - 14
SP  - 13672
VL  - 23
DO  - 10.1007/s11356-015-5205-0
ER  - 

@article{
author = "Nikolić, Nina and Boecker, Reinhard and Nikolic, Miroslav",
year = "2016",
abstract = "Despite the growing popularity of ecological restoration approach, data on primary succession on toxic post-mining substrates, under site environmental conditions which considerably differ from the surrounding environment, are still scarce. Here, we studied the spontaneous vegetation development on an unusual locality created by long-term and large-scale fluvial deposition of sulphidic tailings from a copper mine in a pronouncedly xerothermic, calcareous surrounding. We performed multivariate analyses of soil samples (20 physical and chemical parameters) and vegetation samples (floristic and structural parameters in three types of occurring forests), collected along the pollution gradients throughout the affected floodplain. The nature can cope with two types of imposed constraints: (a) excessive Cu concentrations and (b) very low pH, combined with nutrient deficiency. The former will still allow convergence to the original vegetation, while the latter will result in novel, depauperate assemblages of species typical for cooler and moister climate. Our results for the first time demonstrate that with the increasing severity of environmental filtering, the relative importance of the surrounding vegetation for primary succession strongly decreases.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Long-term passive restoration following fluvial deposition of sulphidic copper tailings: nature filters out the solutions",
pages = "13680-13672",
number = "14",
volume = "23",
doi = "10.1007/s11356-015-5205-0"
}

Nikolić, N., Boecker, R.,& Nikolic, M.. (2016). Long-term passive restoration following fluvial deposition of sulphidic copper tailings: nature filters out the solutions. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 23(14), 13672-13680.
https://doi.org/10.1007/s11356-015-5205-0

Nikolić N, Boecker R, Nikolic M. Long-term passive restoration following fluvial deposition of sulphidic copper tailings: nature filters out the solutions. in Environmental Science and Pollution Research. 2016;23(14):13672-13680.
doi:10.1007/s11356-015-5205-0 .

Nikolić, Nina, Boecker, Reinhard, Nikolic, Miroslav, "Long-term passive restoration following fluvial deposition of sulphidic copper tailings: nature filters out the solutions" in Environmental Science and Pollution Research, 23, no. 14 (2016):13672-13680,
https://doi.org/10.1007/s11356-015-5205-0 . .

TY  - JOUR
AU  - Deljanin, Isidora
AU  - Antanasijević, Davor
AU  - Bjelajac, Anđelika
AU  - Anicic-Urosević, Mira P
AU  - Nikolic, Miroslav
AU  - Peric-Grujic, Aleksandra
AU  - Ristic, Mirjana
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/940
AB  - The concentrations of 15 elements were measured in the leaf samples of Aesculus hippocastanum, Tilia spp., Betula pendula and Acer platanoides collected in May and September of 2014 from four different locations in Belgrade, Serbia. The objective was to assess the chemical characterization of leaf surface and in-wax fractions, as well as the leaf tissue element content, by analyzing untreated, washed with water and washed with chloroform leaf samples, respectively. The combined approach of self-organizing networks (SON) and Preference Ranking Organization Method for Enrichment Evaluation (PROMETHEE) aided by Geometrical Analysis for Interactive Aid (GAIA) was used in the interpretation of multiple element loads on/in the tree leaves. The morphological characteristics of the leaf surfaces and the elemental composition of particulate matter (PM) deposited on tree leaves were studied by using scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS) detector. The results showed that the amounts of retained and accumulated element concentrations depend on several parameters, such as chemical properties of the element and morphological properties of the leaves. Among the studied species, Tilia spp. was found to be the most effective in the accumulation of elements in leaf tissue (70% of the total element concentration), while A. hippocastanum had the lowest accumulation (54%). After water and chloroform washing, the highest percentages of removal were observed for Al, V, Cr, Cu, Zn, As, Cd and Sb (>40%). The PROMETHEE/SONranking/classifying results were in accordance with the results obtained from the GAIA clustering techniques. The combination of the techniques enabled extraction of additional information from datasets. Therefore, the use of both the ranking and clustering methods could be a useful tool to be applied in biomonitoring studies of trace elements.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Chemometrics in biomonitoring: Distribution and correlation of trace elements in tree leaves
EP  - 371
SP  - 361
VL  - 545
DO  - 10.1016/j.scitotenv.2015.12.018
ER  - 

@article{
author = "Deljanin, Isidora and Antanasijević, Davor and Bjelajac, Anđelika and Anicic-Urosević, Mira P and Nikolic, Miroslav and Peric-Grujic, Aleksandra and Ristic, Mirjana",
year = "2016",
abstract = "The concentrations of 15 elements were measured in the leaf samples of Aesculus hippocastanum, Tilia spp., Betula pendula and Acer platanoides collected in May and September of 2014 from four different locations in Belgrade, Serbia. The objective was to assess the chemical characterization of leaf surface and in-wax fractions, as well as the leaf tissue element content, by analyzing untreated, washed with water and washed with chloroform leaf samples, respectively. The combined approach of self-organizing networks (SON) and Preference Ranking Organization Method for Enrichment Evaluation (PROMETHEE) aided by Geometrical Analysis for Interactive Aid (GAIA) was used in the interpretation of multiple element loads on/in the tree leaves. The morphological characteristics of the leaf surfaces and the elemental composition of particulate matter (PM) deposited on tree leaves were studied by using scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS) detector. The results showed that the amounts of retained and accumulated element concentrations depend on several parameters, such as chemical properties of the element and morphological properties of the leaves. Among the studied species, Tilia spp. was found to be the most effective in the accumulation of elements in leaf tissue (70% of the total element concentration), while A. hippocastanum had the lowest accumulation (54%). After water and chloroform washing, the highest percentages of removal were observed for Al, V, Cr, Cu, Zn, As, Cd and Sb (>40%). The PROMETHEE/SONranking/classifying results were in accordance with the results obtained from the GAIA clustering techniques. The combination of the techniques enabled extraction of additional information from datasets. Therefore, the use of both the ranking and clustering methods could be a useful tool to be applied in biomonitoring studies of trace elements.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Chemometrics in biomonitoring: Distribution and correlation of trace elements in tree leaves",
pages = "371-361",
volume = "545",
doi = "10.1016/j.scitotenv.2015.12.018"
}

Deljanin, I., Antanasijević, D., Bjelajac, A., Anicic-Urosević, M. P., Nikolic, M., Peric-Grujic, A.,& Ristic, M.. (2016). Chemometrics in biomonitoring: Distribution and correlation of trace elements in tree leaves. in Science of the Total Environment
Elsevier, Amsterdam., 545, 361-371.
https://doi.org/10.1016/j.scitotenv.2015.12.018

Deljanin I, Antanasijević D, Bjelajac A, Anicic-Urosević MP, Nikolic M, Peric-Grujic A, Ristic M. Chemometrics in biomonitoring: Distribution and correlation of trace elements in tree leaves. in Science of the Total Environment. 2016;545:361-371.
doi:10.1016/j.scitotenv.2015.12.018 .

Deljanin, Isidora, Antanasijević, Davor, Bjelajac, Anđelika, Anicic-Urosević, Mira P, Nikolic, Miroslav, Peric-Grujic, Aleksandra, Ristic, Mirjana, "Chemometrics in biomonitoring: Distribution and correlation of trace elements in tree leaves" in Science of the Total Environment, 545 (2016):361-371,
https://doi.org/10.1016/j.scitotenv.2015.12.018 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Ducic, Tanja
AU  - Grolimund, Daniel
AU  - Bajuk-Bogdanović, Danica
AU  - Nikolic, Miroslav
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Zakrzewska, Joanna
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/862
AB  - Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium
EP  - 7496
IS  - 24
SP  - 7487
VL  - 407
DO  - 10.1007/s00216-015-8916-7
ER  - 

@article{
author = "Žižić, Milan and Ducic, Tanja and Grolimund, Daniel and Bajuk-Bogdanović, Danica and Nikolic, Miroslav and Stanić, Marina and Krizak, Strahinja and Zakrzewska, Joanna",
year = "2015",
abstract = "Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium",
pages = "7496-7487",
number = "24",
volume = "407",
doi = "10.1007/s00216-015-8916-7"
}

Žižić, M., Ducic, T., Grolimund, D., Bajuk-Bogdanović, D., Nikolic, M., Stanić, M., Krizak, S.,& Zakrzewska, J.. (2015). X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 407(24), 7487-7496.
https://doi.org/10.1007/s00216-015-8916-7

Žižić M, Ducic T, Grolimund D, Bajuk-Bogdanović D, Nikolic M, Stanić M, Krizak S, Zakrzewska J. X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry. 2015;407(24):7487-7496.
doi:10.1007/s00216-015-8916-7 .

Žižić, Milan, Ducic, Tanja, Grolimund, Daniel, Bajuk-Bogdanović, Danica, Nikolic, Miroslav, Stanić, Marina, Krizak, Strahinja, Zakrzewska, Joanna, "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium" in Analytical and Bioanalytical Chemistry, 407, no. 24 (2015):7487-7496,
https://doi.org/10.1007/s00216-015-8916-7 . .

TY  - JOUR
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Samardžić, Jelena T.
AU  - Milisavljević, Mira
AU  - Maksimović, Vuk
AU  - Cakmak, Dragan
AU  - Manojlović, Dragan
AU  - Nikolic, Miroslav
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/838
AB  - We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).
PB  - Springer, New York
T2  - Biology and Fertility of Soils
T1  - Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat
EP  - 298
IS  - 3
SP  - 289
VL  - 51
DO  - 10.1007/s00374-014-0975-y
ER  - 

@article{
author = "Kostić Kravljanac, Ljiljana and Nikolić, Nina and Samardžić, Jelena T. and Milisavljević, Mira and Maksimović, Vuk and Cakmak, Dragan and Manojlović, Dragan and Nikolic, Miroslav",
year = "2015",
abstract = "We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).",
publisher = "Springer, New York",
journal = "Biology and Fertility of Soils",
title = "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat",
pages = "298-289",
number = "3",
volume = "51",
doi = "10.1007/s00374-014-0975-y"
}

Kostić Kravljanac, L., Nikolić, N., Samardžić, J. T., Milisavljević, M., Maksimović, V., Cakmak, D., Manojlović, D.,& Nikolic, M.. (2015). Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils
Springer, New York., 51(3), 289-298.
https://doi.org/10.1007/s00374-014-0975-y

Kostić Kravljanac L, Nikolić N, Samardžić JT, Milisavljević M, Maksimović V, Cakmak D, Manojlović D, Nikolic M. Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils. 2015;51(3):289-298.
doi:10.1007/s00374-014-0975-y .

Kostić Kravljanac, Ljiljana, Nikolić, Nina, Samardžić, Jelena T., Milisavljević, Mira, Maksimović, Vuk, Cakmak, Dragan, Manojlović, Dragan, Nikolic, Miroslav, "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat" in Biology and Fertility of Soils, 51, no. 3 (2015):289-298,
https://doi.org/10.1007/s00374-014-0975-y . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Milisavljević, Mira
AU  - Nikolic, Dragana B.
AU  - Milovanović, Bosko M.
AU  - Nikolic, Dragana S.
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/848
AB  - MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by "stem-loop" primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for "stem loop" primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as "stem loop" RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis
EP  - 416
IS  - 2
SP  - 405
VL  - 47
DO  - 10.2298/GENSR1502405T
ER  - 

@article{
author = "Timotijević, Gordana and Milisavljević, Mira and Nikolic, Dragana B. and Milovanović, Bosko M. and Nikolic, Dragana S. and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2015",
abstract = "MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by "stem-loop" primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for "stem loop" primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as "stem loop" RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis",
pages = "416-405",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502405T"
}

Timotijević, G., Milisavljević, M., Nikolic, D. B., Milovanović, B. M., Nikolic, D. S., Nikolic, M.,& Samardžić, J. T.. (2015). Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(2), 405-416.
https://doi.org/10.2298/GENSR1502405T

Timotijević G, Milisavljević M, Nikolic DB, Milovanović BM, Nikolic DS, Nikolic M, Samardžić JT. Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis. in Genetika-Belgrade. 2015;47(2):405-416.
doi:10.2298/GENSR1502405T .

Timotijević, Gordana, Milisavljević, Mira, Nikolic, Dragana B., Milovanović, Bosko M., Nikolic, Dragana S., Nikolic, Miroslav, Samardžić, Jelena T., "Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis" in Genetika-Belgrade, 47, no. 2 (2015):405-416,
https://doi.org/10.2298/GENSR1502405T . .

TY  - JOUR
AU  - Pontigo, Sofia
AU  - Ribera, Alejandra
AU  - Gianfreda, Liliana
AU  - de, la Luz Mora, Maria
AU  - Nikolic, Miroslav
AU  - Cartes, Paula
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/836
AB  - So far, considerable advances have been achieved in understanding the mechanisms of Si uptake and transport in vascular plants. This review presents a comprehensive update about this issue, but also provides the new insights into the role of Si against mineral stresses that occur in acid soils. Such information could be helpful to understand both the differential Si uptake ability as well as the benefits of this mineral element on plants grown under acidic conditions. Silicon (Si) has been widely recognized as a beneficial element for many plant species, especially under stress conditions. In the last few years, great efforts have been made to elucidate the mechanisms involved in uptake and transport of Si by vascular plants and recently, different Si transporters have been identified. Several researches indicate that Si can alleviate various mineral stresses in plants growing under acidic conditions, including aluminium (Al) and manganese (Mn) toxicities as well as phosphorus (P) deficiency all of which are highly detrimental to crop production. This review presents recent findings concerning the influence of uptake and transport of Si on mineral stress under acidic conditions because a knowledge of this interaction provides the basis for understanding the role of Si in mitigating mineral stress in acid soils. Currently, only four Si transporters have been identified and there is little information concerning the response of Si transporters under stress conditions. More investigations are therefore needed to establish whether there is a relationship between Si transporters and the benefits of Si to plants subjected to mineral stress. Evidence presented suggests that Si supply and its subsequent accumulation in plant tissues could be exploited as a strategy to improve crop productivity on acid soils.
PB  - Springer, New York
T2  - Planta
T1  - Silicon in vascular plants: uptake, transport and its influence on mineral stress under acidic conditions
EP  - 37
IS  - 1
SP  - 23
VL  - 242
DO  - 10.1007/s00425-015-2333-1
ER  - 

@article{
author = "Pontigo, Sofia and Ribera, Alejandra and Gianfreda, Liliana and de, la Luz Mora, Maria and Nikolic, Miroslav and Cartes, Paula",
year = "2015",
abstract = "So far, considerable advances have been achieved in understanding the mechanisms of Si uptake and transport in vascular plants. This review presents a comprehensive update about this issue, but also provides the new insights into the role of Si against mineral stresses that occur in acid soils. Such information could be helpful to understand both the differential Si uptake ability as well as the benefits of this mineral element on plants grown under acidic conditions. Silicon (Si) has been widely recognized as a beneficial element for many plant species, especially under stress conditions. In the last few years, great efforts have been made to elucidate the mechanisms involved in uptake and transport of Si by vascular plants and recently, different Si transporters have been identified. Several researches indicate that Si can alleviate various mineral stresses in plants growing under acidic conditions, including aluminium (Al) and manganese (Mn) toxicities as well as phosphorus (P) deficiency all of which are highly detrimental to crop production. This review presents recent findings concerning the influence of uptake and transport of Si on mineral stress under acidic conditions because a knowledge of this interaction provides the basis for understanding the role of Si in mitigating mineral stress in acid soils. Currently, only four Si transporters have been identified and there is little information concerning the response of Si transporters under stress conditions. More investigations are therefore needed to establish whether there is a relationship between Si transporters and the benefits of Si to plants subjected to mineral stress. Evidence presented suggests that Si supply and its subsequent accumulation in plant tissues could be exploited as a strategy to improve crop productivity on acid soils.",
publisher = "Springer, New York",
journal = "Planta",
title = "Silicon in vascular plants: uptake, transport and its influence on mineral stress under acidic conditions",
pages = "37-23",
number = "1",
volume = "242",
doi = "10.1007/s00425-015-2333-1"
}

Pontigo, S., Ribera, A., Gianfreda, L., de, l. L. M. M., Nikolic, M.,& Cartes, P.. (2015). Silicon in vascular plants: uptake, transport and its influence on mineral stress under acidic conditions. in Planta
Springer, New York., 242(1), 23-37.
https://doi.org/10.1007/s00425-015-2333-1

Pontigo S, Ribera A, Gianfreda L, de LLMM, Nikolic M, Cartes P. Silicon in vascular plants: uptake, transport and its influence on mineral stress under acidic conditions. in Planta. 2015;242(1):23-37.
doi:10.1007/s00425-015-2333-1 .

Pontigo, Sofia, Ribera, Alejandra, Gianfreda, Liliana, de, la Luz Mora, Maria, Nikolic, Miroslav, Cartes, Paula, "Silicon in vascular plants: uptake, transport and its influence on mineral stress under acidic conditions" in Planta, 242, no. 1 (2015):23-37,
https://doi.org/10.1007/s00425-015-2333-1 . .

TY  - JOUR
AU  - Nikolić, Nina
AU  - Boecker, Reinhard
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/766
AB  - Questions: Effects of soil on vegetation patterns are commonly obscured by other environmental factors; clear and general relationships are difficult to find. How would community assembly processes be affected by a substantial change in soil characteristics when all other relevant factors are held constant? In particular, can we identify some functional adaptations which would underpin such soil-induced vegetation response? Location: Eastern Serbia: fields partially damaged by long-term and large-scale fluvial deposition of sulphidic waste from a Cu mine; subcontinental/submediterranean climate. Methods: We analysed the multivariate response of cereal weed assemblages (including biomass and foliar analyses) to a strong man-made soil gradient (from highly calcareous to highly acidic, nutrient-poor soils) over short distances (field scale). Results: The soil gradient favoured a substitution of calcicoles by calcifuges, and an increase in abundance of pseudometallophytes, with preferences for Atlantic climate, broad geographical distribution, hemicryptophytic life form, adapted to low-nutrient and acidic soils, with lower concentrations of Ca, and very narrow range of Cu concentrations in leaves. The trends of abundance of the different ecological groups of indicator species along the soil gradient were systematically reflected in the maintenance of leaf P concentrations, and strong homeostasis in biomass N:P ratio. Conclusion: Using annual weed vegetation at the field scale as a fairly simple model, we demonstrated links between gradients in soil properties (pH, nutrient availability) and floristic composition that are normally encountered over large geographic distances. We showed that leaf nutrient status, in particular the maintenance of leaf P concentrations and strong homeostasis of biomass N:P ratio, underpinned a clear functional response of vegetation to mineral stress. These findings can help to understand assembly processes leading to unusual, novel combinations of species which are typically observed as a consequence of strong environmental filtering, as for instance on sites affected by industrial activities.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints
IS  - 12
VL  - 9
DO  - 10.1371/journal.pone.0114290
ER  - 

@article{
author = "Nikolić, Nina and Boecker, Reinhard and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav",
year = "2014",
abstract = "Questions: Effects of soil on vegetation patterns are commonly obscured by other environmental factors; clear and general relationships are difficult to find. How would community assembly processes be affected by a substantial change in soil characteristics when all other relevant factors are held constant? In particular, can we identify some functional adaptations which would underpin such soil-induced vegetation response? Location: Eastern Serbia: fields partially damaged by long-term and large-scale fluvial deposition of sulphidic waste from a Cu mine; subcontinental/submediterranean climate. Methods: We analysed the multivariate response of cereal weed assemblages (including biomass and foliar analyses) to a strong man-made soil gradient (from highly calcareous to highly acidic, nutrient-poor soils) over short distances (field scale). Results: The soil gradient favoured a substitution of calcicoles by calcifuges, and an increase in abundance of pseudometallophytes, with preferences for Atlantic climate, broad geographical distribution, hemicryptophytic life form, adapted to low-nutrient and acidic soils, with lower concentrations of Ca, and very narrow range of Cu concentrations in leaves. The trends of abundance of the different ecological groups of indicator species along the soil gradient were systematically reflected in the maintenance of leaf P concentrations, and strong homeostasis in biomass N:P ratio. Conclusion: Using annual weed vegetation at the field scale as a fairly simple model, we demonstrated links between gradients in soil properties (pH, nutrient availability) and floristic composition that are normally encountered over large geographic distances. We showed that leaf nutrient status, in particular the maintenance of leaf P concentrations and strong homeostasis of biomass N:P ratio, underpinned a clear functional response of vegetation to mineral stress. These findings can help to understand assembly processes leading to unusual, novel combinations of species which are typically observed as a consequence of strong environmental filtering, as for instance on sites affected by industrial activities.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints",
number = "12",
volume = "9",
doi = "10.1371/journal.pone.0114290"
}

Nikolić, N., Boecker, R., Kostić Kravljanac, L.,& Nikolic, M.. (2014). Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints. in PLoS One
Public Library Science, San Francisco., 9(12).
https://doi.org/10.1371/journal.pone.0114290

Nikolić N, Boecker R, Kostić Kravljanac L, Nikolic M. Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints. in PLoS One. 2014;9(12).
doi:10.1371/journal.pone.0114290 .

Nikolić, Nina, Boecker, Reinhard, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, "Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints" in PLoS One, 9, no. 12 (2014),
https://doi.org/10.1371/journal.pone.0114290 . .

TY  - JOUR
AU  - Bityutskii, Nikolai
AU  - Pavlović, Jelena
AU  - Yakkonen, Kirill
AU  - Maksimović, Vuk
AU  - Nikolic, Miroslav
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/763
AB  - Although the beneficial role of silicon (Si) in alleviation of abiotic stress is well established, little is known of the relevance of Si nutrition under microelement deficiency. The aim of our work was to investigate the physiological role of Si in relation to micronutrient (Fe, Zn and Mn) deficiencies in cucumber (Cucumis sativus L). Cucumber (cv. Semkross) plants were grown hydroponically in a complete nutrient solution (control) and in nutrient solutions free from Fe, Zn or Mn, with or without Si supply. Plant tissue concentrations of microelements, organic acids and phenolics were measured. Si supply effectively mitigated the symptoms of Fe deficiency, but only in part, the symptoms of Zn- or Mn deficiency. Leaf Fe concentration significantly increased in plants deprived of Fe but treated with Si, whereas the concentrations of other microelements were not affected by Si supply. The effects of Si supply in increasing accumulation of both organic acids and phenolic compounds in cucumber tissues were exclusively related to Fe nutrition. Enhancement of Fe distribution towards apical shoot parts, along with the tissue accumulation of Fe-mobilizing compounds such as citrate (in leaves and roots) or cathechin (in roots) appears to be the major alleviating effect of Si. Si nutrition, however, was without effect on the mobility and tissue distribution of either Zn or Mn.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber
EP  - 211
SP  - 205
VL  - 74
DO  - 10.1016/j.plaphy.2013.11.015
ER  - 

@article{
author = "Bityutskii, Nikolai and Pavlović, Jelena and Yakkonen, Kirill and Maksimović, Vuk and Nikolic, Miroslav",
year = "2014",
abstract = "Although the beneficial role of silicon (Si) in alleviation of abiotic stress is well established, little is known of the relevance of Si nutrition under microelement deficiency. The aim of our work was to investigate the physiological role of Si in relation to micronutrient (Fe, Zn and Mn) deficiencies in cucumber (Cucumis sativus L). Cucumber (cv. Semkross) plants were grown hydroponically in a complete nutrient solution (control) and in nutrient solutions free from Fe, Zn or Mn, with or without Si supply. Plant tissue concentrations of microelements, organic acids and phenolics were measured. Si supply effectively mitigated the symptoms of Fe deficiency, but only in part, the symptoms of Zn- or Mn deficiency. Leaf Fe concentration significantly increased in plants deprived of Fe but treated with Si, whereas the concentrations of other microelements were not affected by Si supply. The effects of Si supply in increasing accumulation of both organic acids and phenolic compounds in cucumber tissues were exclusively related to Fe nutrition. Enhancement of Fe distribution towards apical shoot parts, along with the tissue accumulation of Fe-mobilizing compounds such as citrate (in leaves and roots) or cathechin (in roots) appears to be the major alleviating effect of Si. Si nutrition, however, was without effect on the mobility and tissue distribution of either Zn or Mn.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber",
pages = "211-205",
volume = "74",
doi = "10.1016/j.plaphy.2013.11.015"
}

Bityutskii, N., Pavlović, J., Yakkonen, K., Maksimović, V.,& Nikolic, M.. (2014). Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 74, 205-211.
https://doi.org/10.1016/j.plaphy.2013.11.015

Bityutskii N, Pavlović J, Yakkonen K, Maksimović V, Nikolic M. Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber. in Plant Physiology and Biochemistry. 2014;74:205-211.
doi:10.1016/j.plaphy.2013.11.015 .

Bityutskii, Nikolai, Pavlović, Jelena, Yakkonen, Kirill, Maksimović, Vuk, Nikolic, Miroslav, "Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber" in Plant Physiology and Biochemistry, 74 (2014):205-211,
https://doi.org/10.1016/j.plaphy.2013.11.015 . .