TY  - JOUR
AU  - Pavun, Leposava
AU  - Uskokovic-Marković, Snežana
AU  - Jelikic-Stankov, Milena
AU  - Đikanović, Daniela
AU  - Durdević, Predrag
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1135
AB  - We propose a sensitive and selective spectrofluorimetric method for the determination of flavonoids as expressed in 'quercetin equivalent' in apple juices. The method is based on the strong emission of the aluminium(III)-quercetin complex at 480 nm with excitation at 420 nm, and it is successfully applied for the determination of flavonoids in commercial apple juices and compared with results obtained in reference spectrophotometric determination. The flavonoid content in commercial apple juices was found to range from 5.53 to 15.55 mg/l quercetin equivalent. The very good agreement between the two methods indicates the suitability of the proposed spectrofluorimetric method for the precise and accurate determination of flavonoids. In addition, the total polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu (FC) method and the antioxidative activity of the tested juices was tested in a DPPH assay and these values were correlated with each other. The obtained profiles of compounds with antioxidative ability lead us to conclude that fruit juice labels based only on fruit % might sometimes misinform consumers.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Czech Journal of Food Sciences
T1  - Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices
EP  - 238
IS  - 3
SP  - 233
VL  - 36
DO  - 10.17221/211/2017-CJFS
ER  - 

@article{
author = "Pavun, Leposava and Uskokovic-Marković, Snežana and Jelikic-Stankov, Milena and Đikanović, Daniela and Durdević, Predrag",
year = "2018",
abstract = "We propose a sensitive and selective spectrofluorimetric method for the determination of flavonoids as expressed in 'quercetin equivalent' in apple juices. The method is based on the strong emission of the aluminium(III)-quercetin complex at 480 nm with excitation at 420 nm, and it is successfully applied for the determination of flavonoids in commercial apple juices and compared with results obtained in reference spectrophotometric determination. The flavonoid content in commercial apple juices was found to range from 5.53 to 15.55 mg/l quercetin equivalent. The very good agreement between the two methods indicates the suitability of the proposed spectrofluorimetric method for the precise and accurate determination of flavonoids. In addition, the total polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu (FC) method and the antioxidative activity of the tested juices was tested in a DPPH assay and these values were correlated with each other. The obtained profiles of compounds with antioxidative ability lead us to conclude that fruit juice labels based only on fruit % might sometimes misinform consumers.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Czech Journal of Food Sciences",
title = "Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices",
pages = "238-233",
number = "3",
volume = "36",
doi = "10.17221/211/2017-CJFS"
}

Pavun, L., Uskokovic-Marković, S., Jelikic-Stankov, M., Đikanović, D.,& Durdević, P.. (2018). Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices. in Czech Journal of Food Sciences
Czech Academy Agricultural Sciences, Prague., 36(3), 233-238.
https://doi.org/10.17221/211/2017-CJFS

Pavun L, Uskokovic-Marković S, Jelikic-Stankov M, Đikanović D, Durdević P. Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices. in Czech Journal of Food Sciences. 2018;36(3):233-238.
doi:10.17221/211/2017-CJFS .

Pavun, Leposava, Uskokovic-Marković, Snežana, Jelikic-Stankov, Milena, Đikanović, Daniela, Durdević, Predrag, "Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices" in Czech Journal of Food Sciences, 36, no. 3 (2018):233-238,
https://doi.org/10.17221/211/2017-CJFS . .

TY  - JOUR
AU  - Milutinović, Milena
AU  - Stanković, Milan
AU  - Cvetković, Danijela
AU  - Maksimović, Vuk
AU  - Smit, Biljana
AU  - Pavlović, Radoslav
AU  - Marković, Snežana
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/870
AB  - Considering that induction of apoptosis is one of the main strategies in cancer therapy, the molecular mechanisms of cytotoxic effects of Allium flavumL. on colon cancer cell lines were investigated and applied in single and co-treatments with new-synthesized Pd(II) complex. The analysis of chemical composition identified caffeic acid glycosides as the most dominant phenolics in A.flavum extract. In all investigated extracts, A.flavum showed cytotoxic effects on colon cancer cells (IC50=1.64-84g/mL) but not on healthy cells. Combinations of plant extracts with Pd(II) complex caused lower IC50 values and better proapoptotic activity. Pd(II) complex induced high percentage of necrosis in a single treatment, but in the combination with plant extracts it had better proapoptotic and lower necrotic activity. Treatments and co-treatments induced higher O-2(-) production and influenced apoptosis biomarkers, leading to Fas protein overexpression and activation of caspases 8 and 9. Practical ApplicationsIn view of the achieved anticancer properties, insufficiently investigated Allium flavum L. is a promising candidate for developing new anticancer compounds and deserves further research and scientific validation. This study has great impact on investigation of new anticancer substances from natural source, focuses on the importance of using plants as the source of medicinal drugs, contributes to the development of the appropriate therapy and gives contribution in both scientific and practical means. Because of its synergistic actions with the newly synthesized chemical anticancer agent and ability to reduce side effects (thus reducing necrotic activity of Pd[II] complex), A.flavum can be used as a dietary food supplement or supplement to chemotherapy perhaps in combination with currently used chemotherapeutics or in some strategies in cancer therapy. This finding is also important from a nutritional point of view because this extracts contain significant amounts of bioactive constituents, which provide health benefits.
PB  - Wiley, Hoboken
T2  - Journal of Food Biochemistry
T1  - The Molecular Mechanisms of Apoptosis Induced by Allium flavumL. and Synergistic Effects with New-Synthesized Pd(II) Complex on Colon Cancer Cells
EP  - 250
IS  - 3
SP  - 238
VL  - 39
DO  - 10.1111/jfbc.12123
ER  - 

@article{
author = "Milutinović, Milena and Stanković, Milan and Cvetković, Danijela and Maksimović, Vuk and Smit, Biljana and Pavlović, Radoslav and Marković, Snežana",
year = "2015",
abstract = "Considering that induction of apoptosis is one of the main strategies in cancer therapy, the molecular mechanisms of cytotoxic effects of Allium flavumL. on colon cancer cell lines were investigated and applied in single and co-treatments with new-synthesized Pd(II) complex. The analysis of chemical composition identified caffeic acid glycosides as the most dominant phenolics in A.flavum extract. In all investigated extracts, A.flavum showed cytotoxic effects on colon cancer cells (IC50=1.64-84g/mL) but not on healthy cells. Combinations of plant extracts with Pd(II) complex caused lower IC50 values and better proapoptotic activity. Pd(II) complex induced high percentage of necrosis in a single treatment, but in the combination with plant extracts it had better proapoptotic and lower necrotic activity. Treatments and co-treatments induced higher O-2(-) production and influenced apoptosis biomarkers, leading to Fas protein overexpression and activation of caspases 8 and 9. Practical ApplicationsIn view of the achieved anticancer properties, insufficiently investigated Allium flavum L. is a promising candidate for developing new anticancer compounds and deserves further research and scientific validation. This study has great impact on investigation of new anticancer substances from natural source, focuses on the importance of using plants as the source of medicinal drugs, contributes to the development of the appropriate therapy and gives contribution in both scientific and practical means. Because of its synergistic actions with the newly synthesized chemical anticancer agent and ability to reduce side effects (thus reducing necrotic activity of Pd[II] complex), A.flavum can be used as a dietary food supplement or supplement to chemotherapy perhaps in combination with currently used chemotherapeutics or in some strategies in cancer therapy. This finding is also important from a nutritional point of view because this extracts contain significant amounts of bioactive constituents, which provide health benefits.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Biochemistry",
title = "The Molecular Mechanisms of Apoptosis Induced by Allium flavumL. and Synergistic Effects with New-Synthesized Pd(II) Complex on Colon Cancer Cells",
pages = "250-238",
number = "3",
volume = "39",
doi = "10.1111/jfbc.12123"
}

Milutinović, M., Stanković, M., Cvetković, D., Maksimović, V., Smit, B., Pavlović, R.,& Marković, S.. (2015). The Molecular Mechanisms of Apoptosis Induced by Allium flavumL. and Synergistic Effects with New-Synthesized Pd(II) Complex on Colon Cancer Cells. in Journal of Food Biochemistry
Wiley, Hoboken., 39(3), 238-250.
https://doi.org/10.1111/jfbc.12123

Milutinović M, Stanković M, Cvetković D, Maksimović V, Smit B, Pavlović R, Marković S. The Molecular Mechanisms of Apoptosis Induced by Allium flavumL. and Synergistic Effects with New-Synthesized Pd(II) Complex on Colon Cancer Cells. in Journal of Food Biochemistry. 2015;39(3):238-250.
doi:10.1111/jfbc.12123 .

Milutinović, Milena, Stanković, Milan, Cvetković, Danijela, Maksimović, Vuk, Smit, Biljana, Pavlović, Radoslav, Marković, Snežana, "The Molecular Mechanisms of Apoptosis Induced by Allium flavumL. and Synergistic Effects with New-Synthesized Pd(II) Complex on Colon Cancer Cells" in Journal of Food Biochemistry, 39, no. 3 (2015):238-250,
https://doi.org/10.1111/jfbc.12123 . .

TY  - JOUR
AU  - Pavun, Leposava
AU  - Durdević, Predrag
AU  - Jelikic-Stankov, Milena
AU  - Đikanović, Daniela
AU  - Ciric, Andrija
AU  - Uskokovic-Marković, Snežana
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/799
AB  - A simple, accurate and precise method based on the fluorescence properties of the aluminum(III)-quercetin complex, for the determination of quercetin, has been developed and validated. The complex has strong emission at pH 3.30, lambda(em) = 480 nm, with lambda(ex) = 420 nm. The linearity range of quercetin determination was 1.5-60.5 ng ml(-1) with LOD 0.09 ng ml(-1) and LOQ 0.27 ng ml(-1). Recovery values in the range of 99.9-100.2% indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with a recovery value of 98.3%, standard deviation of 0.22% and coefficient of variation of 0.09%. The reliability of the method was checked by the newly developed RP-HPLC/UV method for capsules with the direct determination of quercetin after separation. The good agreement between the two methods indicates the applicability of the proposed spectrofluorimetric method for quercetin determination in pharmaceutical dosage forms, with high reproducibility, and enables the direct and simple determination without its prior extraction from samples. The proposed spectrofluorimetric method has much better sensitivity and LOD and LOQ values that are about 1000 times lower than data reported in the literature.
PB  - Soc Chemists Technologists Madeconia, Skopje
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms
EP  - 215
IS  - 2
SP  - 209
VL  - 33
DO  - 10.20450/mjcce.2014.496
ER  - 

@article{
author = "Pavun, Leposava and Durdević, Predrag and Jelikic-Stankov, Milena and Đikanović, Daniela and Ciric, Andrija and Uskokovic-Marković, Snežana",
year = "2014",
abstract = "A simple, accurate and precise method based on the fluorescence properties of the aluminum(III)-quercetin complex, for the determination of quercetin, has been developed and validated. The complex has strong emission at pH 3.30, lambda(em) = 480 nm, with lambda(ex) = 420 nm. The linearity range of quercetin determination was 1.5-60.5 ng ml(-1) with LOD 0.09 ng ml(-1) and LOQ 0.27 ng ml(-1). Recovery values in the range of 99.9-100.2% indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with a recovery value of 98.3%, standard deviation of 0.22% and coefficient of variation of 0.09%. The reliability of the method was checked by the newly developed RP-HPLC/UV method for capsules with the direct determination of quercetin after separation. The good agreement between the two methods indicates the applicability of the proposed spectrofluorimetric method for quercetin determination in pharmaceutical dosage forms, with high reproducibility, and enables the direct and simple determination without its prior extraction from samples. The proposed spectrofluorimetric method has much better sensitivity and LOD and LOQ values that are about 1000 times lower than data reported in the literature.",
publisher = "Soc Chemists Technologists Madeconia, Skopje",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms",
pages = "215-209",
number = "2",
volume = "33",
doi = "10.20450/mjcce.2014.496"
}

Pavun, L., Durdević, P., Jelikic-Stankov, M., Đikanović, D., Ciric, A.,& Uskokovic-Marković, S.. (2014). Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms. in Macedonian Journal of Chemistry and Chemical Engineering
Soc Chemists Technologists Madeconia, Skopje., 33(2), 209-215.
https://doi.org/10.20450/mjcce.2014.496

Pavun L, Durdević P, Jelikic-Stankov M, Đikanović D, Ciric A, Uskokovic-Marković S. Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms. in Macedonian Journal of Chemistry and Chemical Engineering. 2014;33(2):209-215.
doi:10.20450/mjcce.2014.496 .

Pavun, Leposava, Durdević, Predrag, Jelikic-Stankov, Milena, Đikanović, Daniela, Ciric, Andrija, Uskokovic-Marković, Snežana, "Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms" in Macedonian Journal of Chemistry and Chemical Engineering, 33, no. 2 (2014):209-215,
https://doi.org/10.20450/mjcce.2014.496 . .

TY  - JOUR
AU  - Pavun, Leposava A
AU  - Dimitrić-Marković, Jasmina
AU  - Djurdjević, Predrag T
AU  - Jelikic-Stankov, Milena D
AU  - Đikanović, Daniela
AU  - Ciric, Andrija
AU  - Malesev, Dusan L
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/512
AB  - A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 mu g mL(-1) with a limit of detection, LOD, of 0.016 mu g mL(-1) and a limit of quantification, LOQ, of 0.049 mu g mL(-1). Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 mu g mL(-1). The LOD was 0.032 mu g mL(-1) while LOQ was 0.096 mu g mL(-1). Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 mu g mL(-1). The LOD was 0.01 mu g mL(-1) and the LOQ was 0.03 mu g mL(-1). The linearity range for the determination of hesperidin in plasma was 0.02-10.00 mu g mL(-1) with an LOD 0.005 mu g mL(-1) and an LOQ of 0.015 mu g mL(-1). The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms
EP  - 1640
IS  - 11
SP  - 1625
VL  - 77
DO  - 10.2298/JSC111005060P
ER  - 

@article{
author = "Pavun, Leposava A and Dimitrić-Marković, Jasmina and Djurdjević, Predrag T and Jelikic-Stankov, Milena D and Đikanović, Daniela and Ciric, Andrija and Malesev, Dusan L",
year = "2012",
abstract = "A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 mu g mL(-1) with a limit of detection, LOD, of 0.016 mu g mL(-1) and a limit of quantification, LOQ, of 0.049 mu g mL(-1). Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 mu g mL(-1). The LOD was 0.032 mu g mL(-1) while LOQ was 0.096 mu g mL(-1). Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 mu g mL(-1). The LOD was 0.01 mu g mL(-1) and the LOQ was 0.03 mu g mL(-1). The linearity range for the determination of hesperidin in plasma was 0.02-10.00 mu g mL(-1) with an LOD 0.005 mu g mL(-1) and an LOQ of 0.015 mu g mL(-1). The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms",
pages = "1640-1625",
number = "11",
volume = "77",
doi = "10.2298/JSC111005060P"
}

Pavun, L. A., Dimitrić-Marković, J., Djurdjević, P. T., Jelikic-Stankov, M. D., Đikanović, D., Ciric, A.,& Malesev, D. L.. (2012). Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(11), 1625-1640.
https://doi.org/10.2298/JSC111005060P

Pavun LA, Dimitrić-Marković J, Djurdjević PT, Jelikic-Stankov MD, Đikanović D, Ciric A, Malesev DL. Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms. in Journal of the Serbian Chemical Society. 2012;77(11):1625-1640.
doi:10.2298/JSC111005060P .

Pavun, Leposava A, Dimitrić-Marković, Jasmina, Djurdjević, Predrag T, Jelikic-Stankov, Milena D, Đikanović, Daniela, Ciric, Andrija, Malesev, Dusan L, "Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms" in Journal of the Serbian Chemical Society, 77, no. 11 (2012):1625-1640,
https://doi.org/10.2298/JSC111005060P . .