Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices
2018
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Аутори
Pavun, LeposavaUskokovic-Marković, Snežana
Jelikic-Stankov, Milena
Đikanović, Daniela
Durdević, Predrag
Чланак у часопису (Објављена верзија)
Publisher's own license
Метаподаци
Приказ свих података о документуАпстракт
We propose a sensitive and selective spectrofluorimetric method for the determination of flavonoids as expressed in 'quercetin equivalent' in apple juices. The method is based on the strong emission of the aluminium(III)-quercetin complex at 480 nm with excitation at 420 nm, and it is successfully applied for the determination of flavonoids in commercial apple juices and compared with results obtained in reference spectrophotometric determination. The flavonoid content in commercial apple juices was found to range from 5.53 to 15.55 mg/l quercetin equivalent. The very good agreement between the two methods indicates the suitability of the proposed spectrofluorimetric method for the precise and accurate determination of flavonoids. In addition, the total polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu (FC) method and the antioxidative activity of the tested juices was tested in a DPPH assay and these values were correlated with each other. The obtained... profiles of compounds with antioxidative ability lead us to conclude that fruit juice labels based only on fruit % might sometimes misinform consumers.
Кључне речи:
spectrophotometry / spectrofluorimetry / quercetin / antioxidant activity / aluminiumИзвор:
Czech Journal of Food Sciences, 2018, 36, 3, 233-238Издавач:
- Czech Academy Agricultural Sciences, Prague
Финансирање / пројекти:
- Синтеза, моделовање, физичко-хемијске и биолошке особине органских једињења и одговарајућих комплекса метала (RS-MESTD-Basic Research (BR or ON)-172016)
- Електропроводни и редокс-активни полимери и олигомери: синтеза, структура, својства и примена (RS-MESTD-Basic Research (BR or ON)-172043)
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
DOI: 10.17221/211/2017-CJFS
ISSN: 1212-1800
WoS: 000437015500006
Scopus: 2-s2.0-85049382451
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Pavun, Leposava AU - Uskokovic-Marković, Snežana AU - Jelikic-Stankov, Milena AU - Đikanović, Daniela AU - Durdević, Predrag PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1135 AB - We propose a sensitive and selective spectrofluorimetric method for the determination of flavonoids as expressed in 'quercetin equivalent' in apple juices. The method is based on the strong emission of the aluminium(III)-quercetin complex at 480 nm with excitation at 420 nm, and it is successfully applied for the determination of flavonoids in commercial apple juices and compared with results obtained in reference spectrophotometric determination. The flavonoid content in commercial apple juices was found to range from 5.53 to 15.55 mg/l quercetin equivalent. The very good agreement between the two methods indicates the suitability of the proposed spectrofluorimetric method for the precise and accurate determination of flavonoids. In addition, the total polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu (FC) method and the antioxidative activity of the tested juices was tested in a DPPH assay and these values were correlated with each other. The obtained profiles of compounds with antioxidative ability lead us to conclude that fruit juice labels based only on fruit % might sometimes misinform consumers. PB - Czech Academy Agricultural Sciences, Prague T2 - Czech Journal of Food Sciences T1 - Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices EP - 238 IS - 3 SP - 233 VL - 36 DO - 10.17221/211/2017-CJFS ER -
@article{ author = "Pavun, Leposava and Uskokovic-Marković, Snežana and Jelikic-Stankov, Milena and Đikanović, Daniela and Durdević, Predrag", year = "2018", abstract = "We propose a sensitive and selective spectrofluorimetric method for the determination of flavonoids as expressed in 'quercetin equivalent' in apple juices. The method is based on the strong emission of the aluminium(III)-quercetin complex at 480 nm with excitation at 420 nm, and it is successfully applied for the determination of flavonoids in commercial apple juices and compared with results obtained in reference spectrophotometric determination. The flavonoid content in commercial apple juices was found to range from 5.53 to 15.55 mg/l quercetin equivalent. The very good agreement between the two methods indicates the suitability of the proposed spectrofluorimetric method for the precise and accurate determination of flavonoids. In addition, the total polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu (FC) method and the antioxidative activity of the tested juices was tested in a DPPH assay and these values were correlated with each other. The obtained profiles of compounds with antioxidative ability lead us to conclude that fruit juice labels based only on fruit % might sometimes misinform consumers.", publisher = "Czech Academy Agricultural Sciences, Prague", journal = "Czech Journal of Food Sciences", title = "Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices", pages = "238-233", number = "3", volume = "36", doi = "10.17221/211/2017-CJFS" }
Pavun, L., Uskokovic-Marković, S., Jelikic-Stankov, M., Đikanović, D.,& Durdević, P.. (2018). Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices. in Czech Journal of Food Sciences Czech Academy Agricultural Sciences, Prague., 36(3), 233-238. https://doi.org/10.17221/211/2017-CJFS
Pavun L, Uskokovic-Marković S, Jelikic-Stankov M, Đikanović D, Durdević P. Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices. in Czech Journal of Food Sciences. 2018;36(3):233-238. doi:10.17221/211/2017-CJFS .
Pavun, Leposava, Uskokovic-Marković, Snežana, Jelikic-Stankov, Milena, Đikanović, Daniela, Durdević, Predrag, "Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices" in Czech Journal of Food Sciences, 36, no. 3 (2018):233-238, https://doi.org/10.17221/211/2017-CJFS . .