TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica Z.
AU  - Milčić Matić, Natalija
AU  - Krnjaić, Dejan
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2481
AB  - Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious
disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological
analyses of the affected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the affected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing effect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side effects were noticed. Synthesized lignin oligomers may
have a significant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant
staphylococci are found on the skin lesions in animals.
PB  - Springer Nature
T2  - Veterinary Research Communications
T1  - Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study
DO  - 10.1007/s11259-023-10224-9
ER  - 

@article{
author = "Spasojević, Dragica and Zmejkoski, Danica Z. and Milčić Matić, Natalija and Krnjaić, Dejan and Radotić, Ksenija",
year = "2023",
abstract = "Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious
disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological
analyses of the affected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the affected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing effect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side effects were noticed. Synthesized lignin oligomers may
have a significant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant
staphylococci are found on the skin lesions in animals.",
publisher = "Springer Nature",
journal = "Veterinary Research Communications",
title = "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study",
doi = "10.1007/s11259-023-10224-9"
}

Spasojević, D., Zmejkoski, D. Z., Milčić Matić, N., Krnjaić, D.,& Radotić, K.. (2023). Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications
Springer Nature..
https://doi.org/10.1007/s11259-023-10224-9

Spasojević D, Zmejkoski DZ, Milčić Matić N, Krnjaić D, Radotić K. Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications. 2023;.
doi:10.1007/s11259-023-10224-9 .

Spasojević, Dragica, Zmejkoski, Danica Z., Milčić Matić, Natalija, Krnjaić, Dejan, Radotić, Ksenija, "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study" in Veterinary Research Communications (2023),
https://doi.org/10.1007/s11259-023-10224-9 . .

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Mutavdžić, Dragosav
AU  - Šekuljica, Nataša
AU  - Jovanović, Jelena
AU  - Maksimović, Vuk
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2480
AB  - In recent years, versatile peroxidase (VP) has emerged as a promising enzyme for biotechnological applications, as it can oxidize lignin without the external mediators. To gain insights into the breakdown process of artificial lignin by VP, reaction between the two was studied. Degradation products were fractionated using ultrafiltration and analyzed by RP- high performance liquid chromatography with mass detection (HPLC-MS) chromatography. Four fractions were obtained based on their molecular sizes: >10, 3–10, 1–3, and <1 kDa. Interestingly, while VP did not significantly alter the yields of these fractions, the chromatograms revealed the presence of oligomers with different molecular weights (MWs) resulting from the enzymatic activity. The VP exhibits a dual role in its enzymatic activity: both degrading and synthesizing these oligomers. This was confirmed by principal component analysis (PCA). The positive correlations were found between certain oligomers (D1 and D2, D5 and D6, as well as between D7, D10, T2, and T4), suggesting their simultaneous degradation. On the other hand, a negative correlation was found between the monomer and some oligomers (D7, D10, T2, and T4), indicating the decomposition of these oligomers into monomers. These findings shed light on the intricate interplay between VP and artificial lignin, offering valuable insights for potential applications in lignin valorization.
PB  - John Wiley and Sons Inc
T2  - Biotechnology Journal
T1  - Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions
SP  - 2300312
DO  - 10.1002/biot.202300312
ER  - 

@article{
author = "Spasojević, Dragica and Prodanović, Olivera and Mutavdžić, Dragosav and Šekuljica, Nataša and Jovanović, Jelena and Maksimović, Vuk and Radotić, Ksenija",
year = "2023",
abstract = "In recent years, versatile peroxidase (VP) has emerged as a promising enzyme for biotechnological applications, as it can oxidize lignin without the external mediators. To gain insights into the breakdown process of artificial lignin by VP, reaction between the two was studied. Degradation products were fractionated using ultrafiltration and analyzed by RP- high performance liquid chromatography with mass detection (HPLC-MS) chromatography. Four fractions were obtained based on their molecular sizes: >10, 3–10, 1–3, and <1 kDa. Interestingly, while VP did not significantly alter the yields of these fractions, the chromatograms revealed the presence of oligomers with different molecular weights (MWs) resulting from the enzymatic activity. The VP exhibits a dual role in its enzymatic activity: both degrading and synthesizing these oligomers. This was confirmed by principal component analysis (PCA). The positive correlations were found between certain oligomers (D1 and D2, D5 and D6, as well as between D7, D10, T2, and T4), suggesting their simultaneous degradation. On the other hand, a negative correlation was found between the monomer and some oligomers (D7, D10, T2, and T4), indicating the decomposition of these oligomers into monomers. These findings shed light on the intricate interplay between VP and artificial lignin, offering valuable insights for potential applications in lignin valorization.",
publisher = "John Wiley and Sons Inc",
journal = "Biotechnology Journal",
title = "Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions",
pages = "2300312",
doi = "10.1002/biot.202300312"
}

Spasojević, D., Prodanović, O., Mutavdžić, D., Šekuljica, N., Jovanović, J., Maksimović, V.,& Radotić, K.. (2023). Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions. in Biotechnology Journal
John Wiley and Sons Inc., 2300312.
https://doi.org/10.1002/biot.202300312

Spasojević D, Prodanović O, Mutavdžić D, Šekuljica N, Jovanović J, Maksimović V, Radotić K. Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions. in Biotechnology Journal. 2023;:2300312.
doi:10.1002/biot.202300312 .

Spasojević, Dragica, Prodanović, Olivera, Mutavdžić, Dragosav, Šekuljica, Nataša, Jovanović, Jelena, Maksimović, Vuk, Radotić, Ksenija, "Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions" in Biotechnology Journal (2023):2300312,
https://doi.org/10.1002/biot.202300312 . .

TY  - JOUR
AU  - Radotić, Ksenija
AU  - Melø, Thor B.
AU  - Lindgren, Mikael
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2201
AB  - In situ fluorescence measurements have been used to investigate relative amounts of blue-green pigments and
their distributions in plant leaves from Euphorbia pulcherrima. Advantage was taken from the fact that this species
has white leaves on the top, with low pigment concentrations, and green leaves on the stem with ordinary
pigment concentrations. Excitation- and emission spectra below 410 nm from white leaves, where pigment
absorption is low, are not distorted by self-absorption. Absorption- and reflection spectra from white and green
leaves were measured using a spectrophotometer equipped with an integrating sphere. The absorption spectra
were used to correct recorded fluorescence spectra for self-absorption. Self-absorption corrected photosystem
fluorescence from green leaves, modeling light transmission in leaf tissue exponentially, matches to the excitation/
emission spectra from white leaves, apart from small differences due to the pigment concentrations and
selective scattering. The introduced exponentially decaying transmission relation also predicts that the ratio of
excitation spectra from a white and green leaf is in proportion to the absorption spectrum of the green leaf, which
was validated for Photosystem II particle fluorescence. This relation was also used to find a scaled absorption
spectrum responsible for blue-green emission, which was assumed to originate from lignin. Excitation/emission
spectra of the blue-green fluorescence were decomposed into five components and their relative amounts from
adaxial and abaxial sides of the leaves have been quantified. Fluorescence lifetime measurements of the leaves,
PB  - Elsevier
T2  - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
T1  - A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)
EP  - 8
IS  - 123269
SP  - 2
VL  - 303
DO  - 10.1016/j.saa.2023.123269
ER  - 

@article{
author = "Radotić, Ksenija and Melø, Thor B. and Lindgren, Mikael",
year = "2023",
abstract = "In situ fluorescence measurements have been used to investigate relative amounts of blue-green pigments and
their distributions in plant leaves from Euphorbia pulcherrima. Advantage was taken from the fact that this species
has white leaves on the top, with low pigment concentrations, and green leaves on the stem with ordinary
pigment concentrations. Excitation- and emission spectra below 410 nm from white leaves, where pigment
absorption is low, are not distorted by self-absorption. Absorption- and reflection spectra from white and green
leaves were measured using a spectrophotometer equipped with an integrating sphere. The absorption spectra
were used to correct recorded fluorescence spectra for self-absorption. Self-absorption corrected photosystem
fluorescence from green leaves, modeling light transmission in leaf tissue exponentially, matches to the excitation/
emission spectra from white leaves, apart from small differences due to the pigment concentrations and
selective scattering. The introduced exponentially decaying transmission relation also predicts that the ratio of
excitation spectra from a white and green leaf is in proportion to the absorption spectrum of the green leaf, which
was validated for Photosystem II particle fluorescence. This relation was also used to find a scaled absorption
spectrum responsible for blue-green emission, which was assumed to originate from lignin. Excitation/emission
spectra of the blue-green fluorescence were decomposed into five components and their relative amounts from
adaxial and abaxial sides of the leaves have been quantified. Fluorescence lifetime measurements of the leaves,",
publisher = "Elsevier",
journal = "Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy",
title = "A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)",
pages = "8-2",
number = "123269",
volume = "303",
doi = "10.1016/j.saa.2023.123269"
}

Radotić, K., Melø, T. B.,& Lindgren, M.. (2023). A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima). in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Elsevier., 303(123269), 2-8.
https://doi.org/10.1016/j.saa.2023.123269

Radotić K, Melø TB, Lindgren M. A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima). in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2023;303(123269):2-8.
doi:10.1016/j.saa.2023.123269 .

Radotić, Ksenija, Melø, Thor B., Lindgren, Mikael, "A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)" in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 303, no. 123269 (2023):2-8,
https://doi.org/10.1016/j.saa.2023.123269 . .

TY  - CONF
AU  - Perać, Sanja
AU  - Savic, Slavica
AU  - Branković, Zorica
AU  - Bernik, Slavko
AU  - Radojković, Aleksandar
AU  - Branković, Goran
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2213
AB  - With the increase of the consumption of electrical energy, the need for new energy sources is growing. Conversion of waste heat into electricity, based on the thermoelectric effects is one of the ways to produce the electrical energy. Layered cobalt oxides have been the subject of many investigations in past decade as candidates for application in energy conversion. The ceramic sodium cobaltite became a promising candidate for potential thermoelectric applications, because of its large thermopower and low resistivity. In this work, polycrystalline samples of NaCo2–xCuxO4 (x = 0, 0.01, 0.03, 0.05) were synthesized from the powder precursors obtained by the citric acid complex method (CAC) and mechanochemically assisted solid state reaction method (MASSR). The obtained powders were uniaxially pressed into disc-shaped pellets and subsequently sintered at 880 °C in inert argon atmosphere. Thermoelectric parameters (the electrical resistivity (ρ), the thermal conductivity (κ) and the Seebeck coefficient (S)) were measured in two temperature regions. In the first one (between 2 and 300 K) κ and S were measured by a Quantum Design physical property measurement system (PPMS 9T) equipped with a 9 T magnet and ρ by a standard four-terminal technique using the direct current. In the second, all parameters were measured simultaneously, in the temperature gradient (ΔT) between hot and cold sides of the samples using Z- meter, based on the “large ΔT method”, and the figure of merit (ZT) was subsequen tly calculated. Accordingly, ρ, κ and S were determined for a temperature gradient that is established between the hot and cold sides of the samples at the time of each measurement; thus the obtained values represented the actual thermoelectric response of a material under conditions of application. In the low temperature range the highest figure of merit of 0.022 at 300 K was observed for the CAC sample doped with 1 mol% Cu, and it was almost twice higher than in the undoped sample confirming the significant influence of Cu-doping with even small concentrations. As for the results obtained in the temperature gradient, the highest ZT value of 0.061 at T = 473 K was observed for the sample with 5 mol% of Cu prepared by the CAC method. Sample magnetization was measured using a Quantum Design SQUID MPMS-XL-5 magnetometer in zero field cooled (ZFC) and field cooled (FC) regimes, between 2 K and 300 K and in the applied field of 100 Oe. The magnetic susceptibility (χ) of all samples followed the Curie-Weiss law in the temperature range between 50 K and 300 K, while a negative Weiss constant (θ) implied an antiferromagnetic interaction. Indentation experiments were carried out to investigate mechanical properties, therefore, the hardness (H) and the Young's modulus of elasticity (Y) were determined using Agilent Nanoindenter G200. It was found that the highest Y (65.2 GPa) and H (1.41 GPa) were obtained for the CAC sample containing 1 mol% of Cu. These results indicated a significant improvement of mechanical properties even in the case of the sample with the lowest dopant concentration. In general, better thermoelectric and mechanic properties showed the samples synthesized by the CAC method, confirming that fine, homogeneous precursor powders present a good base for obtaining material with improved thermoelectric performances.
PB  - Institut za multidisciplinarna istraživanja Kneza Višeslava 1, 11000 Belgrade, Serbia
C3  - 7th Conference of the Serbian Society for Ceramic Materials
T1  - Thermoelectric Cu doped sodium cobaltite – structural, magnetic and mechanical properties
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2213
ER  - 

@conference{
author = "Perać, Sanja and Savic, Slavica and Branković, Zorica and Bernik, Slavko and Radojković, Aleksandar and Branković, Goran",
year = "2023",
abstract = "With the increase of the consumption of electrical energy, the need for new energy sources is growing. Conversion of waste heat into electricity, based on the thermoelectric effects is one of the ways to produce the electrical energy. Layered cobalt oxides have been the subject of many investigations in past decade as candidates for application in energy conversion. The ceramic sodium cobaltite became a promising candidate for potential thermoelectric applications, because of its large thermopower and low resistivity. In this work, polycrystalline samples of NaCo2–xCuxO4 (x = 0, 0.01, 0.03, 0.05) were synthesized from the powder precursors obtained by the citric acid complex method (CAC) and mechanochemically assisted solid state reaction method (MASSR). The obtained powders were uniaxially pressed into disc-shaped pellets and subsequently sintered at 880 °C in inert argon atmosphere. Thermoelectric parameters (the electrical resistivity (ρ), the thermal conductivity (κ) and the Seebeck coefficient (S)) were measured in two temperature regions. In the first one (between 2 and 300 K) κ and S were measured by a Quantum Design physical property measurement system (PPMS 9T) equipped with a 9 T magnet and ρ by a standard four-terminal technique using the direct current. In the second, all parameters were measured simultaneously, in the temperature gradient (ΔT) between hot and cold sides of the samples using Z- meter, based on the “large ΔT method”, and the figure of merit (ZT) was subsequen tly calculated. Accordingly, ρ, κ and S were determined for a temperature gradient that is established between the hot and cold sides of the samples at the time of each measurement; thus the obtained values represented the actual thermoelectric response of a material under conditions of application. In the low temperature range the highest figure of merit of 0.022 at 300 K was observed for the CAC sample doped with 1 mol% Cu, and it was almost twice higher than in the undoped sample confirming the significant influence of Cu-doping with even small concentrations. As for the results obtained in the temperature gradient, the highest ZT value of 0.061 at T = 473 K was observed for the sample with 5 mol% of Cu prepared by the CAC method. Sample magnetization was measured using a Quantum Design SQUID MPMS-XL-5 magnetometer in zero field cooled (ZFC) and field cooled (FC) regimes, between 2 K and 300 K and in the applied field of 100 Oe. The magnetic susceptibility (χ) of all samples followed the Curie-Weiss law in the temperature range between 50 K and 300 K, while a negative Weiss constant (θ) implied an antiferromagnetic interaction. Indentation experiments were carried out to investigate mechanical properties, therefore, the hardness (H) and the Young's modulus of elasticity (Y) were determined using Agilent Nanoindenter G200. It was found that the highest Y (65.2 GPa) and H (1.41 GPa) were obtained for the CAC sample containing 1 mol% of Cu. These results indicated a significant improvement of mechanical properties even in the case of the sample with the lowest dopant concentration. In general, better thermoelectric and mechanic properties showed the samples synthesized by the CAC method, confirming that fine, homogeneous precursor powders present a good base for obtaining material with improved thermoelectric performances.",
publisher = "Institut za multidisciplinarna istraživanja Kneza Višeslava 1, 11000 Belgrade, Serbia",
journal = "7th Conference of the Serbian Society for Ceramic Materials",
title = "Thermoelectric Cu doped sodium cobaltite – structural, magnetic and mechanical properties",
pages = "59",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2213"
}

Perać, S., Savic, S., Branković, Z., Bernik, S., Radojković, A.,& Branković, G.. (2023). Thermoelectric Cu doped sodium cobaltite – structural, magnetic and mechanical properties. in 7th Conference of the Serbian Society for Ceramic Materials
Institut za multidisciplinarna istraživanja Kneza Višeslava 1, 11000 Belgrade, Serbia., 59.
https://hdl.handle.net/21.15107/rcub_rimsi_2213

Perać S, Savic S, Branković Z, Bernik S, Radojković A, Branković G. Thermoelectric Cu doped sodium cobaltite – structural, magnetic and mechanical properties. in 7th Conference of the Serbian Society for Ceramic Materials. 2023;:59.
https://hdl.handle.net/21.15107/rcub_rimsi_2213 .

Perać, Sanja, Savic, Slavica, Branković, Zorica, Bernik, Slavko, Radojković, Aleksandar, Branković, Goran, "Thermoelectric Cu doped sodium cobaltite – structural, magnetic and mechanical properties" in 7th Conference of the Serbian Society for Ceramic Materials (2023):59,
https://hdl.handle.net/21.15107/rcub_rimsi_2213 .

TY  - CONF
AU  - Malešević, Aleksandar
AU  - Radojković, Aleksandar
AU  - Žunić, Milan
AU  - Savic, Slavica
AU  - Perać, Sanja
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2215
AB  - Acceptor-doped perovskites (ABO3 general formula) with large lattice constants (such as BaCeO3, SrCeO3, and BaZrO3) have been known as fast proton conductors. The ability to conduct protons at high temperatures makes them suitable for humidity sensors in a high-temperature environment. The presence of traces of humidity can play a key role in the functioning of certain industrial processes at higher temperatures. Electrical characteristics of BaCe0.75In0.25O3–δ (BCI25) sintered sample were analyzed in a dry and a wet argon atmosphere in the 250–700 °C temperature range. The water vapor sensing properties of BCI25 porous film and its response and recovery times were investigated under different conditions of temperature and water vapor concentration. A 30 μm thick film obtained from the powder calcined at 1050 °C exhibited sensitivity comparable to that of the sintered sample with significantly shorter response and recovery times. While the sensitivity of the film gradually decreased with a decrease in partial pressure of water vapor (p(H2O)), a noticeable sensitivity was still observed at p(H2O) of 200 Pa. Decrease in conductivity depended logarithmically on the partial pressure of water with the slope of 0.52 that is close to the theoretical value. After several cycles, the reusability test proved an almost unchanged ratio between the impedance value in the dry and the wet Ar atmosphere (p(H2O) = 2.34 kPa), which implied that BCI25, having good stability and sensitivity, is a promising high-temperature humidity sensor.
PB  - Institut za multidisciplinarna istraživanja Kneza Višeslava 1, 11000 Belgrade, Serbia
C3  - 7th Conference of the Serbian Society for Ceramic Materials
T1  - High temperature humidity sensing ability of indium-doped barium cerate
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2215
ER  - 

@conference{
author = "Malešević, Aleksandar and Radojković, Aleksandar and Žunić, Milan and Savic, Slavica and Perać, Sanja and Branković, Zorica and Branković, Goran",
year = "2023",
abstract = "Acceptor-doped perovskites (ABO3 general formula) with large lattice constants (such as BaCeO3, SrCeO3, and BaZrO3) have been known as fast proton conductors. The ability to conduct protons at high temperatures makes them suitable for humidity sensors in a high-temperature environment. The presence of traces of humidity can play a key role in the functioning of certain industrial processes at higher temperatures. Electrical characteristics of BaCe0.75In0.25O3–δ (BCI25) sintered sample were analyzed in a dry and a wet argon atmosphere in the 250–700 °C temperature range. The water vapor sensing properties of BCI25 porous film and its response and recovery times were investigated under different conditions of temperature and water vapor concentration. A 30 μm thick film obtained from the powder calcined at 1050 °C exhibited sensitivity comparable to that of the sintered sample with significantly shorter response and recovery times. While the sensitivity of the film gradually decreased with a decrease in partial pressure of water vapor (p(H2O)), a noticeable sensitivity was still observed at p(H2O) of 200 Pa. Decrease in conductivity depended logarithmically on the partial pressure of water with the slope of 0.52 that is close to the theoretical value. After several cycles, the reusability test proved an almost unchanged ratio between the impedance value in the dry and the wet Ar atmosphere (p(H2O) = 2.34 kPa), which implied that BCI25, having good stability and sensitivity, is a promising high-temperature humidity sensor.",
publisher = "Institut za multidisciplinarna istraživanja Kneza Višeslava 1, 11000 Belgrade, Serbia",
journal = "7th Conference of the Serbian Society for Ceramic Materials",
title = "High temperature humidity sensing ability of indium-doped barium cerate",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2215"
}

Malešević, A., Radojković, A., Žunić, M., Savic, S., Perać, S., Branković, Z.,& Branković, G.. (2023). High temperature humidity sensing ability of indium-doped barium cerate. in 7th Conference of the Serbian Society for Ceramic Materials
Institut za multidisciplinarna istraživanja Kneza Višeslava 1, 11000 Belgrade, Serbia., 76.
https://hdl.handle.net/21.15107/rcub_rimsi_2215

Malešević A, Radojković A, Žunić M, Savic S, Perać S, Branković Z, Branković G. High temperature humidity sensing ability of indium-doped barium cerate. in 7th Conference of the Serbian Society for Ceramic Materials. 2023;:76.
https://hdl.handle.net/21.15107/rcub_rimsi_2215 .

Malešević, Aleksandar, Radojković, Aleksandar, Žunić, Milan, Savic, Slavica, Perać, Sanja, Branković, Zorica, Branković, Goran, "High temperature humidity sensing ability of indium-doped barium cerate" in 7th Conference of the Serbian Society for Ceramic Materials (2023):76,
https://hdl.handle.net/21.15107/rcub_rimsi_2215 .

TY  - JOUR
AU  - Milojkov, Dušan
AU  - Sokić, Miroslav
AU  - Živković-Radovanović, Vukosava
AU  - Manojlović, Vaso
AU  - Mutavdžić, Dragosav
AU  - Janjić, Goran
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2631
AB  - In this study, luminescence and antibacterial behavior of pure fluorapatite (FAp) and praseodymium-
nitrate-carbonate co-doped fluorapatite (PrNCFAp) nanopowders were investigated.
Uniform nanopowders were synthesized by precipitation reaction followed by
centrifugation and systematically characterized by XRD, FTIR, SEM–EDS, TG and PL
methods. XRD analysis revealed the formation of hexagonal FAp crystals, and FTIR spectra
indicate the presence of nitrate (
NO3
−) and carbonate (
CO3
2−) species. SEM analyzes
confirm agglomerates composed of irregular nanometer-sized spheres. Emission of FAp
nanopowder occurred in the violet-blue region of the visible part of the spectrum, with
redshift to the blue-green color region when Pr3+,
NO3
− and CO3
2− co-doped in the lattice.
Analysis of luminescence spectra by MCR-ALS method extract three fluorophores from
the PrNCFAp sample and showed simultaneous existents of emission-reabsorption-emission
between dopants in FAp lattice. Antibacterial activity against pathogen Staphylococcus
aureus was investigated before and after treatment of nanopowders by UVA radiation
of 365 nm. Nanopowders irradiated with UVA compared to non-irradiated reduced Staphylococcus
aureus by 84.9% for PrNCFAp and 33.3% for FAp in the first 0.5 h of contact, and
76.1% and 42.9% after 24 h of contact. In addition, the obtained luminescent nanomaterials
showed a low degree of hemolytic activity and could potentially be candidates for further
research in dentistry.
PB  - Springer
T2  - Optical and Quantum Electronics
T1  - Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions
SP  - 55:84
DO  - 10.1007/s11082-022-04347-7
ER  - 

@article{
author = "Milojkov, Dušan and Sokić, Miroslav and Živković-Radovanović, Vukosava and Manojlović, Vaso and Mutavdžić, Dragosav and Janjić, Goran and Radotić, Ksenija",
year = "2023",
abstract = "In this study, luminescence and antibacterial behavior of pure fluorapatite (FAp) and praseodymium-
nitrate-carbonate co-doped fluorapatite (PrNCFAp) nanopowders were investigated.
Uniform nanopowders were synthesized by precipitation reaction followed by
centrifugation and systematically characterized by XRD, FTIR, SEM–EDS, TG and PL
methods. XRD analysis revealed the formation of hexagonal FAp crystals, and FTIR spectra
indicate the presence of nitrate (
NO3
−) and carbonate (
CO3
2−) species. SEM analyzes
confirm agglomerates composed of irregular nanometer-sized spheres. Emission of FAp
nanopowder occurred in the violet-blue region of the visible part of the spectrum, with
redshift to the blue-green color region when Pr3+,
NO3
− and CO3
2− co-doped in the lattice.
Analysis of luminescence spectra by MCR-ALS method extract three fluorophores from
the PrNCFAp sample and showed simultaneous existents of emission-reabsorption-emission
between dopants in FAp lattice. Antibacterial activity against pathogen Staphylococcus
aureus was investigated before and after treatment of nanopowders by UVA radiation
of 365 nm. Nanopowders irradiated with UVA compared to non-irradiated reduced Staphylococcus
aureus by 84.9% for PrNCFAp and 33.3% for FAp in the first 0.5 h of contact, and
76.1% and 42.9% after 24 h of contact. In addition, the obtained luminescent nanomaterials
showed a low degree of hemolytic activity and could potentially be candidates for further
research in dentistry.",
publisher = "Springer",
journal = "Optical and Quantum Electronics",
title = "Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions",
pages = "55:84",
doi = "10.1007/s11082-022-04347-7"
}

Milojkov, D., Sokić, M., Živković-Radovanović, V., Manojlović, V., Mutavdžić, D., Janjić, G.,& Radotić, K.. (2023). Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions. in Optical and Quantum Electronics
Springer., 55:84.
https://doi.org/10.1007/s11082-022-04347-7

Milojkov D, Sokić M, Živković-Radovanović V, Manojlović V, Mutavdžić D, Janjić G, Radotić K. Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions. in Optical and Quantum Electronics. 2023;:55:84.
doi:10.1007/s11082-022-04347-7 .

Milojkov, Dušan, Sokić, Miroslav, Živković-Radovanović, Vukosava, Manojlović, Vaso, Mutavdžić, Dragosav, Janjić, Goran, Radotić, Ksenija, "Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions" in Optical and Quantum Electronics (2023):55:84,
https://doi.org/10.1007/s11082-022-04347-7 . .

TY  - JOUR
AU  - Milojkov, Dušan
AU  - Radosavljević-Mihajlović, Ana S
AU  - Stanić, Vojislav Dj
AU  - Nastasijević, Branislav
AU  - Radotić, Ksenija
AU  - Janković Častvan, Ivona
AU  - Živković-Radovanović, Vukosava
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2649
AB  - Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential
next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized
by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible
properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate
the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images
confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis
shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure.
Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal
lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence
in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and
Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic
action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample
in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three
species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium
growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of
Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F�����
ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples
were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad–
spectrum antimicrobial agents for various biomedical and health care preparations.
PB  - Elsevier
T2  - Journal of Photochemistry & Photobiology, B: Biology
T1  - Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents
SP  - 112649
VL  - 239
DO  - 10.1016/j.jphotobiol.2023.112649
ER  - 

@article{
author = "Milojkov, Dušan and Radosavljević-Mihajlović, Ana S and Stanić, Vojislav Dj and Nastasijević, Branislav and Radotić, Ksenija and Janković Častvan, Ivona and Živković-Radovanović, Vukosava",
year = "2023",
abstract = "Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential
next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized
by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible
properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate
the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images
confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis
shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure.
Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal
lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence
in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and
Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic
action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample
in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three
species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium
growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of
Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F�����
ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples
were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad–
spectrum antimicrobial agents for various biomedical and health care preparations.",
publisher = "Elsevier",
journal = "Journal of Photochemistry & Photobiology, B: Biology",
title = "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents",
pages = "112649",
volume = "239",
doi = "10.1016/j.jphotobiol.2023.112649"
}

Milojkov, D., Radosavljević-Mihajlović, A. S., Stanić, V. D., Nastasijević, B., Radotić, K., Janković Častvan, I.,& Živković-Radovanović, V.. (2023). Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry & Photobiology, B: Biology
Elsevier., 239, 112649.
https://doi.org/10.1016/j.jphotobiol.2023.112649

Milojkov D, Radosavljević-Mihajlović AS, Stanić VD, Nastasijević B, Radotić K, Janković Častvan I, Živković-Radovanović V. Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry & Photobiology, B: Biology. 2023;239:112649.
doi:10.1016/j.jphotobiol.2023.112649 .

Milojkov, Dušan, Radosavljević-Mihajlović, Ana S, Stanić, Vojislav Dj, Nastasijević, Branislav, Radotić, Ksenija, Janković Častvan, Ivona, Živković-Radovanović, Vukosava, "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents" in Journal of Photochemistry & Photobiology, B: Biology, 239 (2023):112649,
https://doi.org/10.1016/j.jphotobiol.2023.112649 . .

TY  - CHAP
AU  - Radotić, Ksenija
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica Z.
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2289
AB  - The material selected for biomedical application must be biocompatible, stable against physiological media, non-toxic, non-carcinogenic, corrosion-resistant, bio-inert, and stimuli-responsive, and have a low wear rate. Lignin is the most abundant aromatic polymer on Earth with a complex, recalcitrant structure. A lot of knowledge is acquired on its pre-treatments and processing for biomedical uses in the forms of hydrogels, films, composite materials, nanoparticles, and aerogels. To avoid the potential toxicity and to control the cytotoxicity of lignin-based materials, it is necessary to increase the purity of the starting source materials and understand their reactivity. The poor water solubility, broad molecular weight (MW) distribution, and highly complex structure of lignin restrict its wider clinical applications. These limitations may be effectively improved by chemical modifications or by using lignin fractions with a narrow MW range. Antioxidant and antimicrobial properties allow lignin to be used in pharmaceuticals, drug delivery, and wound healing. It is also reported as a good candidate for composite materials intended for bone regeneration scaffolds in tissue engineering. Future challenges for lignin-based materials for biomedical applications include achieving a better understanding of the structure, isolation, and batch properties of lignin, as well as structure–function relationships in its applications.
PB  - The Royal Society of Chemistry
T2  - Lignin-based Materials: Health Care and Medical Applications
T1  - Lignin-based material for biomedical applications: Basic requirements and properties
EP  - 105
SP  - 85
DO  - 10.1039/9781839167843
ER  - 

@inbook{
author = "Radotić, Ksenija and Spasojević, Dragica and Zmejkoski, Danica Z.",
year = "2023",
abstract = "The material selected for biomedical application must be biocompatible, stable against physiological media, non-toxic, non-carcinogenic, corrosion-resistant, bio-inert, and stimuli-responsive, and have a low wear rate. Lignin is the most abundant aromatic polymer on Earth with a complex, recalcitrant structure. A lot of knowledge is acquired on its pre-treatments and processing for biomedical uses in the forms of hydrogels, films, composite materials, nanoparticles, and aerogels. To avoid the potential toxicity and to control the cytotoxicity of lignin-based materials, it is necessary to increase the purity of the starting source materials and understand their reactivity. The poor water solubility, broad molecular weight (MW) distribution, and highly complex structure of lignin restrict its wider clinical applications. These limitations may be effectively improved by chemical modifications or by using lignin fractions with a narrow MW range. Antioxidant and antimicrobial properties allow lignin to be used in pharmaceuticals, drug delivery, and wound healing. It is also reported as a good candidate for composite materials intended for bone regeneration scaffolds in tissue engineering. Future challenges for lignin-based materials for biomedical applications include achieving a better understanding of the structure, isolation, and batch properties of lignin, as well as structure–function relationships in its applications.",
publisher = "The Royal Society of Chemistry",
journal = "Lignin-based Materials: Health Care and Medical Applications",
booktitle = "Lignin-based material for biomedical applications: Basic requirements and properties",
pages = "105-85",
doi = "10.1039/9781839167843"
}

Radotić, K., Spasojević, D.,& Zmejkoski, D. Z.. (2023). Lignin-based material for biomedical applications: Basic requirements and properties. in Lignin-based Materials: Health Care and Medical Applications
The Royal Society of Chemistry., 85-105.
https://doi.org/10.1039/9781839167843

Radotić K, Spasojević D, Zmejkoski DZ. Lignin-based material for biomedical applications: Basic requirements and properties. in Lignin-based Materials: Health Care and Medical Applications. 2023;:85-105.
doi:10.1039/9781839167843 .

Radotić, Ksenija, Spasojević, Dragica, Zmejkoski, Danica Z., "Lignin-based material for biomedical applications: Basic requirements and properties" in Lignin-based Materials: Health Care and Medical Applications (2023):85-105,
https://doi.org/10.1039/9781839167843 . .

TY  - CONF
AU  - Perać, Sanja
AU  - Savic, Slavica
AU  - Branković, Zorica
AU  - Bernik, Slavko
AU  - Kojic, Sanja
AU  - Vasiljevic, Dragana
AU  - Branković, Goran
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2301
AB  - Ceramic samples of NaCo2-xCuxO4 (x = 0, 0.01, 0.03, 0.05) were obtained after calcination of powder precursors synthesized by a mechanochemically assisted solid-state reaction method (MASSR) and a citric acid complex method (CAC). Effects of small concentrations of Cu doping and the above-mentioned syntheses procedures on the microstructural, thermoelectric and mechanical properties were observed. The electrical resistivity (ρ), the thermal conductivity (κ) and the Seebeck coefficient (S) were measured simultaneously in the temperature gradient (ΔT) between hot and cold side of the sample, and the figure of merit (ZT) was
subsequently calculated. ZT of the CAC samples was higher compared with the MASSR samples. The highest ZT value of 0.061 at ΔT = 473 K was obtained for the sample with 5 mol% of Cu prepared by the CAC method, and it was 1.7 times higher than the highest value obtained for the MASSR sample with 3 mol% of Cu
(ZT = 0.036 at ΔT = 473 K). The CAC samples showed better mechanical properties compared to the MASSR samples due to the higher hardness of the CAC samples which is a consequence of homogeneous microstructure and higher density obtained after sintering of these samples. The results confirmed that, besides the concentration of Cu, the synthesis procedure considerably affected the microstructural, thermoelectric and mechanical properties of NaCo2O4 ceramics.
PB  - Institut za multidisciplinarna istraživanja Kneza Višeslava 1, 11000 Belgrade, Serbia
C3  - 6th Conference of the Serbian Society for Ceramic Materials
T1  - 6.	Effect of Cu dopping on microstructural, thermoelectric and mechanical properties of NaCoCuO4 ceramics
SP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2301
ER  - 

@conference{
author = "Perać, Sanja and Savic, Slavica and Branković, Zorica and Bernik, Slavko and Kojic, Sanja and Vasiljevic, Dragana and Branković, Goran",
year = "2022",
abstract = "Ceramic samples of NaCo2-xCuxO4 (x = 0, 0.01, 0.03, 0.05) were obtained after calcination of powder precursors synthesized by a mechanochemically assisted solid-state reaction method (MASSR) and a citric acid complex method (CAC). Effects of small concentrations of Cu doping and the above-mentioned syntheses procedures on the microstructural, thermoelectric and mechanical properties were observed. The electrical resistivity (ρ), the thermal conductivity (κ) and the Seebeck coefficient (S) were measured simultaneously in the temperature gradient (ΔT) between hot and cold side of the sample, and the figure of merit (ZT) was
subsequently calculated. ZT of the CAC samples was higher compared with the MASSR samples. The highest ZT value of 0.061 at ΔT = 473 K was obtained for the sample with 5 mol% of Cu prepared by the CAC method, and it was 1.7 times higher than the highest value obtained for the MASSR sample with 3 mol% of Cu
(ZT = 0.036 at ΔT = 473 K). The CAC samples showed better mechanical properties compared to the MASSR samples due to the higher hardness of the CAC samples which is a consequence of homogeneous microstructure and higher density obtained after sintering of these samples. The results confirmed that, besides the concentration of Cu, the synthesis procedure considerably affected the microstructural, thermoelectric and mechanical properties of NaCo2O4 ceramics.",
publisher = "Institut za multidisciplinarna istraživanja Kneza Višeslava 1, 11000 Belgrade, Serbia",
journal = "6th Conference of the Serbian Society for Ceramic Materials",
title = "6.	Effect of Cu dopping on microstructural, thermoelectric and mechanical properties of NaCoCuO4 ceramics",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2301"
}

Perać, S., Savic, S., Branković, Z., Bernik, S., Kojic, S., Vasiljevic, D.,& Branković, G.. (2022). 6.	Effect of Cu dopping on microstructural, thermoelectric and mechanical properties of NaCoCuO4 ceramics. in 6th Conference of the Serbian Society for Ceramic Materials
Institut za multidisciplinarna istraživanja Kneza Višeslava 1, 11000 Belgrade, Serbia., 87.
https://hdl.handle.net/21.15107/rcub_rimsi_2301

Perać S, Savic S, Branković Z, Bernik S, Kojic S, Vasiljevic D, Branković G. 6.	Effect of Cu dopping on microstructural, thermoelectric and mechanical properties of NaCoCuO4 ceramics. in 6th Conference of the Serbian Society for Ceramic Materials. 2022;:87.
https://hdl.handle.net/21.15107/rcub_rimsi_2301 .

Perać, Sanja, Savic, Slavica, Branković, Zorica, Bernik, Slavko, Kojic, Sanja, Vasiljevic, Dragana, Branković, Goran, "6.	Effect of Cu dopping on microstructural, thermoelectric and mechanical properties of NaCoCuO4 ceramics" in 6th Conference of the Serbian Society for Ceramic Materials (2022):87,
https://hdl.handle.net/21.15107/rcub_rimsi_2301 .

TY  - GEN
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2494
AB  - Plant cell wall mechanics and its relationship to growth and environmental stress are not entirely revealed. By using atomic force microscopy stiffness tomography we monitored stiffness distribution in the cell wall layers and its evolution during growth phases of living single Arabidopsis cells. In the beginning and end of the cell growth, the average stiffness of the cell wall was low and the wall was mechanically homogenous, while in the exponential growth phase the average wall stiffness increased, with increasing heterogeneity and polysaccharide/lignin content shown by Fourier-transform infrared (FTIR) spectroscopy. In adult plants, we studied mechanical stimuli-induced compression/tension reaction in the tree stems, and thigmotropic twining of the vines. Confocal- and fluorescence detected linear dichroism (FDLD) microscopy revealed that in juvenile spruce  stems under bending stress cell wall organisation changed.  Our spectroscopic, FTIR- and FDLD microscopic studies of parenchima cell walls in Dioscorea vine stem mechanics showed that mechanical strain changes polysaccharid/lignin ratio and organisation,cellulose fibril order staying unchanged.
PB  - Springer Nature
T2  - Book of abstracts
T1  - Plant cell walls - mechanical and chemical modifications underpin growth and stress response
EP  - S60
SP  - S60
DO  - 10.1007/s00249-017-1220-z
ER  - 

@misc{
author = "Radotić, Ksenija",
year = "2018",
abstract = "Plant cell wall mechanics and its relationship to growth and environmental stress are not entirely revealed. By using atomic force microscopy stiffness tomography we monitored stiffness distribution in the cell wall layers and its evolution during growth phases of living single Arabidopsis cells. In the beginning and end of the cell growth, the average stiffness of the cell wall was low and the wall was mechanically homogenous, while in the exponential growth phase the average wall stiffness increased, with increasing heterogeneity and polysaccharide/lignin content shown by Fourier-transform infrared (FTIR) spectroscopy. In adult plants, we studied mechanical stimuli-induced compression/tension reaction in the tree stems, and thigmotropic twining of the vines. Confocal- and fluorescence detected linear dichroism (FDLD) microscopy revealed that in juvenile spruce  stems under bending stress cell wall organisation changed.  Our spectroscopic, FTIR- and FDLD microscopic studies of parenchima cell walls in Dioscorea vine stem mechanics showed that mechanical strain changes polysaccharid/lignin ratio and organisation,cellulose fibril order staying unchanged.",
publisher = "Springer Nature",
journal = "Book of abstracts",
title = "Plant cell walls - mechanical and chemical modifications underpin growth and stress response",
pages = "S60-S60",
doi = "10.1007/s00249-017-1220-z"
}

Radotić, K.. (2018). Plant cell walls - mechanical and chemical modifications underpin growth and stress response. in Book of abstracts
Springer Nature., S60-S60.
https://doi.org/10.1007/s00249-017-1220-z

Radotić K. Plant cell walls - mechanical and chemical modifications underpin growth and stress response. in Book of abstracts. 2018;:S60-S60.
doi:10.1007/s00249-017-1220-z .

Radotić, Ksenija, "Plant cell walls - mechanical and chemical modifications underpin growth and stress response" in Book of abstracts (2018):S60-S60,
https://doi.org/10.1007/s00249-017-1220-z . .

TY  - CHAP
AU  - Radotić, Ksenija
AU  - Mićić, Miodrag
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2465
AB  - Isolation of the two most abundant plant macromolecules, lignin and
cellulose, from the plant tissues is reviewed. Isolation methods depend on the plant
tissue that is used as the source of the polymers. Assessment of purity has an important
place in the whole isolation process. Extent of needed purity depends on the
further application of the obtained polymers. Different lignin extraction procedures
are presented. Lignin isolation via preceding extraction of pulp is usually applied on
wood as a starting material. The procedure preceded by cell wall extraction and
combined with lignin complexation with thioglicolic acid is used for plant tissues
containing various kinds of cells or low lignin content comparing with protein content.
Lignin purifi cation with ionic liquids, as well as those obtaining Brauns’ native
lignin, kraft lignin, or lignosulfonate are also described. The review of cellulose
isolation procedures is presented, such as the alkaline procedure, as well as the
method using ultrasound treatment, or the method applying enzyme technology.
Dilute acid pretreatment in cellulose isolation is also depicted.
PB  - Springer Protocols Handbooks
T2  - Springer Protocols Handbooks
T1  - Methods for Extraction and Purification of Lignin and Cellulose from Plant Tissues
EP  - 376
SP  - 365
DO  - 10.1007/978-1-4939-3185-9_26
ER  - 

@inbook{
author = "Radotić, Ksenija and Mićić, Miodrag",
year = "2016",
abstract = "Isolation of the two most abundant plant macromolecules, lignin and
cellulose, from the plant tissues is reviewed. Isolation methods depend on the plant
tissue that is used as the source of the polymers. Assessment of purity has an important
place in the whole isolation process. Extent of needed purity depends on the
further application of the obtained polymers. Different lignin extraction procedures
are presented. Lignin isolation via preceding extraction of pulp is usually applied on
wood as a starting material. The procedure preceded by cell wall extraction and
combined with lignin complexation with thioglicolic acid is used for plant tissues
containing various kinds of cells or low lignin content comparing with protein content.
Lignin purifi cation with ionic liquids, as well as those obtaining Brauns’ native
lignin, kraft lignin, or lignosulfonate are also described. The review of cellulose
isolation procedures is presented, such as the alkaline procedure, as well as the
method using ultrasound treatment, or the method applying enzyme technology.
Dilute acid pretreatment in cellulose isolation is also depicted.",
publisher = "Springer Protocols Handbooks",
journal = "Springer Protocols Handbooks",
booktitle = "Methods for Extraction and Purification of Lignin and Cellulose from Plant Tissues",
pages = "376-365",
doi = "10.1007/978-1-4939-3185-9_26"
}

Radotić, K.,& Mićić, M.. (2016). Methods for Extraction and Purification of Lignin and Cellulose from Plant Tissues. in Springer Protocols Handbooks
Springer Protocols Handbooks., 365-376.
https://doi.org/10.1007/978-1-4939-3185-9_26

Radotić K, Mićić M. Methods for Extraction and Purification of Lignin and Cellulose from Plant Tissues. in Springer Protocols Handbooks. 2016;:365-376.
doi:10.1007/978-1-4939-3185-9_26 .

Radotić, Ksenija, Mićić, Miodrag, "Methods for Extraction and Purification of Lignin and Cellulose from Plant Tissues" in Springer Protocols Handbooks (2016):365-376,
https://doi.org/10.1007/978-1-4939-3185-9_26 . .