A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)
Само за регистроване кориснике
2023
Чланак у часопису (Објављена верзија)
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Приказ свих података о документуАпстракт
In situ fluorescence measurements have been used to investigate relative amounts of blue-green pigments and
their distributions in plant leaves from Euphorbia pulcherrima. Advantage was taken from the fact that this species
has white leaves on the top, with low pigment concentrations, and green leaves on the stem with ordinary
pigment concentrations. Excitation- and emission spectra below 410 nm from white leaves, where pigment
absorption is low, are not distorted by self-absorption. Absorption- and reflection spectra from white and green
leaves were measured using a spectrophotometer equipped with an integrating sphere. The absorption spectra
were used to correct recorded fluorescence spectra for self-absorption. Self-absorption corrected photosystem
fluorescence from green leaves, modeling light transmission in leaf tissue exponentially, matches to the excitation/
emission spectra from white leaves, apart from small differences due to the pigment concentrations and
selective... scattering. The introduced exponentially decaying transmission relation also predicts that the ratio of
excitation spectra from a white and green leaf is in proportion to the absorption spectrum of the green leaf, which
was validated for Photosystem II particle fluorescence. This relation was also used to find a scaled absorption
spectrum responsible for blue-green emission, which was assumed to originate from lignin. Excitation/emission
spectra of the blue-green fluorescence were decomposed into five components and their relative amounts from
adaxial and abaxial sides of the leaves have been quantified. Fluorescence lifetime measurements of the leaves,
Кључне речи:
Euphorbia pulcherrima / Leaf pigments / Lignin / Light transmission / Absorption spectroscopy / Fluorescence spectroscopyИзвор:
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2023, 303, 123269, 2-8Издавач:
- Elsevier
Финансирање / пројекти:
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Radotić, Ksenija AU - Melø, Thor B. AU - Lindgren, Mikael PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2201 AB - In situ fluorescence measurements have been used to investigate relative amounts of blue-green pigments and their distributions in plant leaves from Euphorbia pulcherrima. Advantage was taken from the fact that this species has white leaves on the top, with low pigment concentrations, and green leaves on the stem with ordinary pigment concentrations. Excitation- and emission spectra below 410 nm from white leaves, where pigment absorption is low, are not distorted by self-absorption. Absorption- and reflection spectra from white and green leaves were measured using a spectrophotometer equipped with an integrating sphere. The absorption spectra were used to correct recorded fluorescence spectra for self-absorption. Self-absorption corrected photosystem fluorescence from green leaves, modeling light transmission in leaf tissue exponentially, matches to the excitation/ emission spectra from white leaves, apart from small differences due to the pigment concentrations and selective scattering. The introduced exponentially decaying transmission relation also predicts that the ratio of excitation spectra from a white and green leaf is in proportion to the absorption spectrum of the green leaf, which was validated for Photosystem II particle fluorescence. This relation was also used to find a scaled absorption spectrum responsible for blue-green emission, which was assumed to originate from lignin. Excitation/emission spectra of the blue-green fluorescence were decomposed into five components and their relative amounts from adaxial and abaxial sides of the leaves have been quantified. Fluorescence lifetime measurements of the leaves, PB - Elsevier T2 - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy T1 - A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima) EP - 8 IS - 123269 SP - 2 VL - 303 DO - 10.1016/j.saa.2023.123269 ER -
@article{ author = "Radotić, Ksenija and Melø, Thor B. and Lindgren, Mikael", year = "2023", abstract = "In situ fluorescence measurements have been used to investigate relative amounts of blue-green pigments and their distributions in plant leaves from Euphorbia pulcherrima. Advantage was taken from the fact that this species has white leaves on the top, with low pigment concentrations, and green leaves on the stem with ordinary pigment concentrations. Excitation- and emission spectra below 410 nm from white leaves, where pigment absorption is low, are not distorted by self-absorption. Absorption- and reflection spectra from white and green leaves were measured using a spectrophotometer equipped with an integrating sphere. The absorption spectra were used to correct recorded fluorescence spectra for self-absorption. Self-absorption corrected photosystem fluorescence from green leaves, modeling light transmission in leaf tissue exponentially, matches to the excitation/ emission spectra from white leaves, apart from small differences due to the pigment concentrations and selective scattering. The introduced exponentially decaying transmission relation also predicts that the ratio of excitation spectra from a white and green leaf is in proportion to the absorption spectrum of the green leaf, which was validated for Photosystem II particle fluorescence. This relation was also used to find a scaled absorption spectrum responsible for blue-green emission, which was assumed to originate from lignin. Excitation/emission spectra of the blue-green fluorescence were decomposed into five components and their relative amounts from adaxial and abaxial sides of the leaves have been quantified. Fluorescence lifetime measurements of the leaves,", publisher = "Elsevier", journal = "Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy", title = "A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)", pages = "8-2", number = "123269", volume = "303", doi = "10.1016/j.saa.2023.123269" }
Radotić, K., Melø, T. B.,& Lindgren, M.. (2023). A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima). in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Elsevier., 303(123269), 2-8. https://doi.org/10.1016/j.saa.2023.123269
Radotić K, Melø TB, Lindgren M. A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima). in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2023;303(123269):2-8. doi:10.1016/j.saa.2023.123269 .
Radotić, Ksenija, Melø, Thor B., Lindgren, Mikael, "A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)" in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 303, no. 123269 (2023):2-8, https://doi.org/10.1016/j.saa.2023.123269 . .