TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Blagojević, Milan
AU  - Bagi, Ferenc
AU  - Vrandečić, Karolina
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2115
AB  - Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA
EP  - 176
IS  - 1
SP  - 159
VL  - 55
DO  - 10.2298/GENSR230159I
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Blagojević, Milan and Bagi, Ferenc and Vrandečić, Karolina and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA",
pages = "176-159",
number = "1",
volume = "55",
doi = "10.2298/GENSR230159I"
}

Iličić, R., Jelušić, A., Blagojević, M., Bagi, F., Vrandečić, K., Stanković, S.,& Popović Milovanović, T.. (2023). CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA. in Genetika
Serbian Genetics Society., 55(1), 159-176.
https://doi.org/10.2298/GENSR230159I

Iličić R, Jelušić A, Blagojević M, Bagi F, Vrandečić K, Stanković S, Popović Milovanović T. CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA. in Genetika. 2023;55(1):159-176.
doi:10.2298/GENSR230159I .

Iličić, Renata, Jelušić, Aleksandra, Blagojević, Milan, Bagi, Ferenc, Vrandečić, Karolina, Stanković, Slaviša, Popović Milovanović, Tatjana, "CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA" in Genetika, 55, no. 1 (2023):159-176,
https://doi.org/10.2298/GENSR230159I . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Medić, Olja
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2116
AB  - Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists’ potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.
PB  - Elsevier Inc.
T2  - Biological Control
T1  - Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4
EP  - 13
SP  - 105238
SP  - 1
VL  - 182
DO  - 10.1016/j.biocontrol.2023.105238
ER  - 

@article{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Medić, Olja and Berić, Tanja and Stanković, Slaviša",
year = "2023",
abstract = "Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists’ potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.",
publisher = "Elsevier Inc.",
journal = "Biological Control",
title = "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4",
pages = "13-105238-1",
volume = "182",
doi = "10.1016/j.biocontrol.2023.105238"
}

Marković, S., Popović Milovanović, T., Jelušić, A., Iličić, R., Medić, O., Berić, T.,& Stanković, S.. (2023). Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control
Elsevier Inc.., 182, 105238-13.
https://doi.org/10.1016/j.biocontrol.2023.105238

Marković S, Popović Milovanović T, Jelušić A, Iličić R, Medić O, Berić T, Stanković S. Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control. 2023;182:105238-13.
doi:10.1016/j.biocontrol.2023.105238 .

Marković, Sanja, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Medić, Olja, Berić, Tanja, Stanković, Slaviša, "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4" in Biological Control, 182 (2023):105238-13,
https://doi.org/10.1016/j.biocontrol.2023.105238 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2117
AB  - The aim of this study was to establish a link between genetic diversity and the geographic
origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - MDPI
T2  - Microorganisms
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
SP  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic
origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8",
pages = "2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms
MDPI., 11(8), 2122.
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms. 2023;11(8):2122.
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms, 11, no. 8 (2023):2122,
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Žečević, Katarina
AU  - Stanisavljević, Rade
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2118
AB  - The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy.
PB  - British Society for Plant Pathology
PB  - Wiley
T2  - Plant Pathology
T1  - Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans
EP  - 299
IS  - 2
SP  - 290
VL  - 72
DO  - 10.1111/ppa.13658
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Milovanović, Predrag and Stanković, Slaviša and Žečević, Katarina and Stanisavljević, Rade and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy.",
publisher = "British Society for Plant Pathology, Wiley",
journal = "Plant Pathology",
title = "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans",
pages = "299-290",
number = "2",
volume = "72",
doi = "10.1111/ppa.13658"
}

Iličić, R., Jelušić, A., Milovanović, P., Stanković, S., Žečević, K., Stanisavljević, R.,& Popović Milovanović, T.. (2023). Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in Plant Pathology
British Society for Plant Pathology., 72(2), 290-299.
https://doi.org/10.1111/ppa.13658

Iličić R, Jelušić A, Milovanović P, Stanković S, Žečević K, Stanisavljević R, Popović Milovanović T. Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in Plant Pathology. 2023;72(2):290-299.
doi:10.1111/ppa.13658 .

Iličić, Renata, Jelušić, Aleksandra, Milovanović, Predrag, Stanković, Slaviša, Žečević, Katarina, Stanisavljević, Rade, Popović Milovanović, Tatjana, "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans" in Plant Pathology, 72, no. 2 (2023):290-299,
https://doi.org/10.1111/ppa.13658 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1919
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible
for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo
F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of
cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were
observed during August 2021, while the inner tissues were macerated, featuring cream to black
discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence
ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP)
medium were characterized for their phenotypic and genotypic features. The pathogenicity was
confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase
chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as
well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus
sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were
identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as
Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating
the presence of diverse Pectobacterium species even in combined infection in the same field. Among
the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and
P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus
sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA,
dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the
phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was
found to be the most virulent species for cabbage, followed by P. versatile, while all three species
had comparable virulence with respect to potato. The results obtained in this work provide a better
understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible
for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo
F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of
cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were
observed during August 2021, while the inner tissues were macerated, featuring cream to black
discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence
ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP)
medium were characterized for their phenotypic and genotypic features. The pathogenicity was
confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase
chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as
well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus
sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were
identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as
Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating
the presence of diverse Pectobacterium species even in combined infection in the same field. Among
the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and
P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus
sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA,
dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the
phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was
found to be the most virulent species for cabbage, followed by P. versatile, while all three species
had comparable virulence with respect to potato. The results obtained in this work provide a better
understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms
MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Marković S, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia" in Microorganisms, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Bagi, Ferenc
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1542
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia
IS  - 1
VL  - 106
DO  - 10.1094/PDIS-06-21-1128-PDN
ER  - 

@article{
author = "Marković, Sanja and Milić Komić, Sonja and Jelušić, Aleksandra and Ilicic, Renata and Bagi, Ferenc and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia",
number = "1",
volume = "106",
doi = "10.1094/PDIS-06-21-1128-PDN"
}

Marković, S., Milić Komić, S., Jelušić, A., Ilicic, R., Bagi, F., Stanković, S.,& Popović, T.. (2022). First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 106(1).
https://doi.org/10.1094/PDIS-06-21-1128-PDN

Marković S, Milić Komić S, Jelušić A, Ilicic R, Bagi F, Stanković S, Popović T. First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease. 2022;106(1).
doi:10.1094/PDIS-06-21-1128-PDN .

Marković, Sanja, Milić Komić, Sonja, Jelušić, Aleksandra, Ilicic, Renata, Bagi, Ferenc, Stanković, Slaviša, Popović, Tatjana, "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia" in Plant Disease, 106, no. 1 (2022),
https://doi.org/10.1094/PDIS-06-21-1128-PDN . .

TY  - CONF
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Trkulja, Nenad
AU  - Živković, Ivana
AU  - Stanković, Slaviša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2138
AB  - The biocontrol ability of Bacillus halotolerans strain B33 against the most significant stone fruit bacterial and fungal pathogens was investigated under in vitro conditions. The results indicate that the inhibition zone toward bacterial pathogen Xanthomonas arboricola pv. pruni originating from peach and apricot (strains Xp219 and Xp320, respectively) ranged from 20 to 45 mm in diameter,
depending of the used B. halotolerans concentration. B. halotolerans B33 did not inhibit the growth of bacteria Pseudomonas syringae pv. syringae (strains RE05 and RE3 originating from sweet cherry), P. s. pv. morsprunorum race 1 (strain Pm5 originating from sweet cherry and Pm26 from plum) and P. cerasi (strain RE10 originating from wild cherry). In the case of fungal pathogen 
Monilinia fructicola strain 116, 41.66−61.00% growth inhibition was observed when pure culture of B. halotolerans was used. From the obtained results in this work, B. halotolerans strain B33 could be recommended to be potentially used as a suitable biocontrol agent for the control of Xanthomonads plant pathogenic bacteria and Monilinia spp. on stone fruit.
PB  - The Balkans Scientific Center of the Russian Academy of Natural Sciences Belgrade
C3  - 4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection
T1  - BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS
EP  - 179
SP  - 170
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2138
ER  - 

@conference{
author = "Iličić, Renata and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Bagi, Ferenc and Trkulja, Nenad and Živković, Ivana and Stanković, Slaviša",
year = "2022",
abstract = "The biocontrol ability of Bacillus halotolerans strain B33 against the most significant stone fruit bacterial and fungal pathogens was investigated under in vitro conditions. The results indicate that the inhibition zone toward bacterial pathogen Xanthomonas arboricola pv. pruni originating from peach and apricot (strains Xp219 and Xp320, respectively) ranged from 20 to 45 mm in diameter,
depending of the used B. halotolerans concentration. B. halotolerans B33 did not inhibit the growth of bacteria Pseudomonas syringae pv. syringae (strains RE05 and RE3 originating from sweet cherry), P. s. pv. morsprunorum race 1 (strain Pm5 originating from sweet cherry and Pm26 from plum) and P. cerasi (strain RE10 originating from wild cherry). In the case of fungal pathogen 
Monilinia fructicola strain 116, 41.66−61.00% growth inhibition was observed when pure culture of B. halotolerans was used. From the obtained results in this work, B. halotolerans strain B33 could be recommended to be potentially used as a suitable biocontrol agent for the control of Xanthomonads plant pathogenic bacteria and Monilinia spp. on stone fruit.",
publisher = "The Balkans Scientific Center of the Russian Academy of Natural Sciences Belgrade",
journal = "4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection",
title = "BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS",
pages = "179-170",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2138"
}

Iličić, R., Popović Milovanović, T., Jelušić, A., Bagi, F., Trkulja, N., Živković, I.,& Stanković, S.. (2022). BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS. in 4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection
The Balkans Scientific Center of the Russian Academy of Natural Sciences Belgrade., 170-179.
https://hdl.handle.net/21.15107/rcub_rimsi_2138

Iličić R, Popović Milovanović T, Jelušić A, Bagi F, Trkulja N, Živković I, Stanković S. BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS. in 4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection. 2022;:170-179.
https://hdl.handle.net/21.15107/rcub_rimsi_2138 .

Iličić, Renata, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Bagi, Ferenc, Trkulja, Nenad, Živković, Ivana, Stanković, Slaviša, "BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS" in 4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection (2022):170-179,
https://hdl.handle.net/21.15107/rcub_rimsi_2138 .

TY  - CONF
AU  - Iličić, Renata
AU  - Barać, Goran
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1949
AB  - BACKGROUND
Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated 
symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were 
frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was 
initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality.
OBJECTIVES
This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) 
in locality Maglić (Bačka) in 2021. 
METHODS 
Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves 
and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium 
bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal 
phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited 
strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to 
reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database.
RESULTS 
PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three 
(two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% 
identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited 
strains of Ca. Phytoplasma solani.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology
T1  - NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE
SP  - 916
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1949
ER  - 

@conference{
author = "Iličić, Renata and Barać, Goran and Marković, Sanja and Jelušić, Aleksandra and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated 
symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were 
frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was 
initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality.
OBJECTIVES
This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) 
in locality Maglić (Bačka) in 2021. 
METHODS 
Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves 
and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium 
bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal 
phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited 
strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to 
reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database.
RESULTS 
PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three 
(two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% 
identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited 
strains of Ca. Phytoplasma solani.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology",
title = "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE",
pages = "916",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1949"
}

Iličić, R., Barać, G., Marković, S., Jelušić, A., Stanković, S.,& Popović, T.. (2022). NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 916.
https://hdl.handle.net/21.15107/rcub_rimsi_1949

Iličić R, Barać G, Marković S, Jelušić A, Stanković S, Popović T. NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:916.
https://hdl.handle.net/21.15107/rcub_rimsi_1949 .

Iličić, Renata, Barać, Goran, Marković, Sanja, Jelušić, Aleksandra, Stanković, Slaviša, Popović, Tatjana, "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE" in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology (2022):916,
https://hdl.handle.net/21.15107/rcub_rimsi_1949 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1951
AB  - BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)
SP  - 915
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1951
ER  - 

@conference{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)",
pages = "915",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1951"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Stanković, S.,& Popović, T.. (2022). PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951

Jelušić A, Mitrović P, Marković S, Iličić R, Stanković S, Popović T. PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Stanković, Slaviša, Popović, Tatjana, "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):915,
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1914
AB  - Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.
PB  - Springer
T2  - Potato Research
T1  - Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres
EP  - 18
SP  - 1
DO  - 10.1007/s11540-022-09601-9
ER  - 

@article{
author = "Marković, Sanja and Popović, Tatjana and Berić, Tanja and Dimkić, Ivica and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2022",
abstract = "Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.",
publisher = "Springer",
journal = "Potato Research",
title = "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres",
pages = "18-1",
doi = "10.1007/s11540-022-09601-9"
}

Marković, S., Popović, T., Berić, T., Dimkić, I., Jelušić, A., Iličić, R.,& Stanković, S.. (2022). Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research
Springer., 1-18.
https://doi.org/10.1007/s11540-022-09601-9

Marković S, Popović T, Berić T, Dimkić I, Jelušić A, Iličić R, Stanković S. Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research. 2022;:1-18.
doi:10.1007/s11540-022-09601-9 .

Marković, Sanja, Popović, Tatjana, Berić, Tanja, Dimkić, Ivica, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres" in Potato Research (2022):1-18,
https://doi.org/10.1007/s11540-022-09601-9 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Ilicic, Renata
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1438
AB  - Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers
EP  - 1959
IS  - 8
SP  - 1945
VL  - 70
DO  - 10.1111/ppa.13421
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Ilicic, Renata and Veljović-Jovanović, Sonja and Milić Komić, Sonja and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers",
pages = "1959-1945",
number = "8",
volume = "70",
doi = "10.1111/ppa.13421"
}

Marković, S., Stanković, S., Ilicic, R., Veljović-Jovanović, S., Milić Komić, S., Jelušić, A.,& Popović, T.. (2021). Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology
Wiley, Hoboken., 70(8), 1945-1959.
https://doi.org/10.1111/ppa.13421

Marković S, Stanković S, Ilicic R, Veljović-Jovanović S, Milić Komić S, Jelušić A, Popović T. Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology. 2021;70(8):1945-1959.
doi:10.1111/ppa.13421 .

Marković, Sanja, Stanković, Slaviša, Ilicic, Renata, Veljović-Jovanović, Sonja, Milić Komić, Sonja, Jelušić, Aleksandra, Popović, Tatjana, "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers" in Plant Pathology, 70, no. 8 (2021):1945-1959,
https://doi.org/10.1111/ppa.13421 . .

TY  - CONF
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1961
AB  - Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, 
jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 
1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium 
atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na 
sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima 
i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši 
identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi 
distribucija populacija u 2020. i 2021. godini.
Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 
uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa 
sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 
ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti 
VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste 
kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira 
i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze 
(PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti 
su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa 
krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano 
(MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. 
Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su 
formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata 
izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama 
krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na 
mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od 
inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, 
izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. 
brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati 
prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata 
sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja 
crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je 
trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.
PB  - Društvo za zaštitu bilja Srbije
C3  - XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
T1  - NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1961
ER  - 

@conference{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović, Tatjana",
year = "2021",
abstract = "Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, 
jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 
1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium 
atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na 
sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima 
i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši 
identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi 
distribucija populacija u 2020. i 2021. godini.
Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 
uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa 
sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 
ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti 
VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste 
kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira 
i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze 
(PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti 
su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa 
krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano 
(MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. 
Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su 
formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata 
izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama 
krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na 
mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od 
inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, 
izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. 
brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati 
prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata 
sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja 
crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je 
trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova",
title = "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1961"
}

Marković, S., Stanković, S., Jelušić, A., Iličić, R.,& Popović, T.. (2021). NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
Društvo za zaštitu bilja Srbije., 17.
https://hdl.handle.net/21.15107/rcub_rimsi_1961

Marković S, Stanković S, Jelušić A, Iličić R, Popović T. NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova. 2021;:17.
https://hdl.handle.net/21.15107/rcub_rimsi_1961 .

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović, Tatjana, "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI" in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova (2021):17,
https://hdl.handle.net/21.15107/rcub_rimsi_1961 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Kosovac, Andrea
AU  - Postic, Dobrivoj
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1494
AB  - Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Backa region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia
EP  - 1090
IS  - 4
SP  - 1080
VL  - 105
DO  - 10.1094/PDIS-05-20-1076-RE
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Ilicic, Renata and Kosovac, Andrea and Postic, Dobrivoj and Popović, Tatjana",
year = "2021",
abstract = "Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Backa region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia",
pages = "1090-1080",
number = "4",
volume = "105",
doi = "10.1094/PDIS-05-20-1076-RE"
}

Marković, S., Stanković, S., Jelušić, A., Ilicic, R., Kosovac, A., Postic, D.,& Popović, T.. (2021). Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 105(4), 1080-1090.
https://doi.org/10.1094/PDIS-05-20-1076-RE

Marković S, Stanković S, Jelušić A, Ilicic R, Kosovac A, Postic D, Popović T. Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia. in Plant Disease. 2021;105(4):1080-1090.
doi:10.1094/PDIS-05-20-1076-RE .

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Ilicic, Renata, Kosovac, Andrea, Postic, Dobrivoj, Popović, Tatjana, "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia" in Plant Disease, 105, no. 4 (2021):1080-1090,
https://doi.org/10.1094/PDIS-05-20-1076-RE . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Peeters, Kelly
AU  - Miklavcic-Visnjevec, Ana
AU  - Tavzes, Crtomir
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1426
AB  - Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates
VL  - 160
DO  - 10.1016/j.biocontrol.2021.104695
ER  - 

@article{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Peeters, Kelly and Miklavcic-Visnjevec, Ana and Tavzes, Crtomir and Stanković, Slaviša and Berić, Tanja",
year = "2021",
abstract = "Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates",
volume = "160",
doi = "10.1016/j.biocontrol.2021.104695"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Peeters, K., Miklavcic-Visnjevec, A., Tavzes, C., Stanković, S.,& Berić, T.. (2021). Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 160.
https://doi.org/10.1016/j.biocontrol.2021.104695

Jelušić A, Popović T, Dimkić I, Mitrović P, Peeters K, Miklavcic-Visnjevec A, Tavzes C, Stanković S, Berić T. Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control. 2021;160.
doi:10.1016/j.biocontrol.2021.104695 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Peeters, Kelly, Miklavcic-Visnjevec, Ana, Tavzes, Crtomir, Stanković, Slaviša, Berić, Tanja, "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates" in Biological Control, 160 (2021),
https://doi.org/10.1016/j.biocontrol.2021.104695 . .

TY  - JOUR
AU  - Ilicic, Renata
AU  - Popović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Vlajic, Slobodan
AU  - Stanković, Slaviša
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1419
AB  - Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia
EP  - 262
IS  - 1
SP  - 247
VL  - 53
DO  - 10.2298/GENSR2101247I
ER  - 

@article{
author = "Ilicic, Renata and Popović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Bagi, Ferenc and Vlajic, Slobodan and Stanković, Slaviša",
year = "2021",
abstract = "Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia",
pages = "262-247",
number = "1",
volume = "53",
doi = "10.2298/GENSR2101247I"
}

Ilicic, R., Popović, T., Marković, S., Jelušić, A., Bagi, F., Vlajic, S.,& Stanković, S.. (2021). Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 53(1), 247-262.
https://doi.org/10.2298/GENSR2101247I

Ilicic R, Popović T, Marković S, Jelušić A, Bagi F, Vlajic S, Stanković S. Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade. 2021;53(1):247-262.
doi:10.2298/GENSR2101247I .

Ilicic, Renata, Popović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Bagi, Ferenc, Vlajic, Slobodan, Stanković, Slaviša, "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia" in Genetika-Belgrade, 53, no. 1 (2021):247-262,
https://doi.org/10.2298/GENSR2101247I . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marjanović-Jeromela, Ana
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1407
AB  - Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia
EP  - 49
IS  - 1
SP  - 35
VL  - 70
DO  - 10.1111/ppa.13273
ER  - 

@article{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marjanović-Jeromela, Ana and Popović, Tatjana",
year = "2021",
abstract = "Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia",
pages = "49-35",
number = "1",
volume = "70",
doi = "10.1111/ppa.13273"
}

Jelušić, A., Berić, T., Mitrović, P., Dimkić, I., Stanković, S., Marjanović-Jeromela, A.,& Popović, T.. (2021). New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia. in Plant Pathology
Wiley, Hoboken., 70(1), 35-49.
https://doi.org/10.1111/ppa.13273

Jelušić A, Berić T, Mitrović P, Dimkić I, Stanković S, Marjanović-Jeromela A, Popović T. New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia. in Plant Pathology. 2021;70(1):35-49.
doi:10.1111/ppa.13273 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marjanović-Jeromela, Ana, Popović, Tatjana, "New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia" in Plant Pathology, 70, no. 1 (2021):35-49,
https://doi.org/10.1111/ppa.13273 . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Popović, Tatjana
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1950
AB  - Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1950
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Popović, Tatjana",
year = "2020",
abstract = "Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA",
pages = "133",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1950"
}

Jelušić, A., Berić, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Popović, T.. (2020). IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950

Jelušić A, Berić T, Dimkić I, Mitrović P, Stanković S, Marković S, Popović T. IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology. 2020;:133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .

Jelušić, Aleksandra, Berić, Tanja, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Popović, Tatjana, "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA" in FEMS Online Conference on Microbiology (2020):133,
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1954
AB  - Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1954
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2020",
abstract = "Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1954"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Berić, T.. (2020). PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954

Jelušić A, Popović T, Dimkić I, Mitrović P, Stanković S, Marković S, Berić T. PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology. 2020;:134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS" in FEMS Online Conference on Microbiology (2020):134,
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .

TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1953
AB  - Background: Bacterial pathogens Ralstonia solanacearum, Clavibacter michiganensis subsp. sepedonicus and Dickeya 
dianthicola are quarantine bacteria in EPPO region and lead to severity losses in potato production. Widespread Pecto bacterium carotovorum subsp. brasiliensis also causes damages under favorable conditions. The available bactericides 
are not enough effective, therefore biological agents in their control are emerging as a possible solution. 
Objectives: The aim of this study was evaluation of antibacterial activity of Bacillus amyloliquefaciens against recently 
found bacteria in Serbian potatoes (R. solanacearum, D. dianthicola and P. c. subsp. brasiliensis).
Methods: Two antagonistic B. amyloliquefaciens strains coded as SS-12.6 and SS-38.4 were used in in vitro screening of 
antimicrobial activity against R. solanacearum (strain Rs81/18), D. dianthicola (strain Dd31) and P. c. subsp. brasiliensis 
(strain Pcb62). Pathogens and antagonists strains were grown in LB medium for 48 h at 26 C° and 30 °C, respectively. 
Supernatants and suspended pellet of full culture of SS-12.6 and SS-38.4 were tested by well diffusion assay and drop plate method, respectively. Diameter of inhibition zones were measured and expressed in mm. Biofilm formation was 
performed on microtitre plates and measured by multi-well plate readers. 
Results: Supernatants of antagonists formed inhibition zones for R. solanacearum (SS-12.6 10×10 mm; SS-38.4 11×11 
mm) and P. c. subsp. brasiliensis (SS-12.6 10×10 mm; 38.4 12×12 mm), while result for D. dianthicola was negative. 
Pellet of full antagonist cultures produced inhibition zones only for R. solanacearum (SS-12.6 10×11 mm; 38.4 11×11 
mm). The biofilm were formed in poor formation for all tested pathogens (category 1).
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS
SP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1953
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2020",
abstract = "Background: Bacterial pathogens Ralstonia solanacearum, Clavibacter michiganensis subsp. sepedonicus and Dickeya 
dianthicola are quarantine bacteria in EPPO region and lead to severity losses in potato production. Widespread Pecto bacterium carotovorum subsp. brasiliensis also causes damages under favorable conditions. The available bactericides 
are not enough effective, therefore biological agents in their control are emerging as a possible solution. 
Objectives: The aim of this study was evaluation of antibacterial activity of Bacillus amyloliquefaciens against recently 
found bacteria in Serbian potatoes (R. solanacearum, D. dianthicola and P. c. subsp. brasiliensis).
Methods: Two antagonistic B. amyloliquefaciens strains coded as SS-12.6 and SS-38.4 were used in in vitro screening of 
antimicrobial activity against R. solanacearum (strain Rs81/18), D. dianthicola (strain Dd31) and P. c. subsp. brasiliensis 
(strain Pcb62). Pathogens and antagonists strains were grown in LB medium for 48 h at 26 C° and 30 °C, respectively. 
Supernatants and suspended pellet of full culture of SS-12.6 and SS-38.4 were tested by well diffusion assay and drop plate method, respectively. Diameter of inhibition zones were measured and expressed in mm. Biofilm formation was 
performed on microtitre plates and measured by multi-well plate readers. 
Results: Supernatants of antagonists formed inhibition zones for R. solanacearum (SS-12.6 10×10 mm; SS-38.4 11×11 
mm) and P. c. subsp. brasiliensis (SS-12.6 10×10 mm; 38.4 12×12 mm), while result for D. dianthicola was negative. 
Pellet of full antagonist cultures produced inhibition zones only for R. solanacearum (SS-12.6 10×11 mm; 38.4 11×11 
mm). The biofilm were formed in poor formation for all tested pathogens (category 1).",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS",
pages = "178",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1953"
}

Marković, S., Popović, T., Jelušić, A., Iličić, R.,& Stanković, S.. (2020). POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 178.
https://hdl.handle.net/21.15107/rcub_rimsi_1953

Marković S, Popović T, Jelušić A, Iličić R, Stanković S. POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS. in FEMS Online Conference on Microbiology. 2020;:178.
https://hdl.handle.net/21.15107/rcub_rimsi_1953 .

Marković, Sanja, Popović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "POTENTIAL OF BACILUS AMYLOLIQUEFACIENS STRAINS SS-12.6 AND SS-38.4 IN BIOLOGICAL CONTROL OF POTATO ROT PATHOGENS" in FEMS Online Conference on Microbiology (2020):178,
https://hdl.handle.net/21.15107/rcub_rimsi_1953 .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Živković, Ljiljana
AU  - Živković, Natalija
AU  - Ilicic, Renata
AU  - Stanisavljević, Rade
AU  - Stanković, Slaviša
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1350
AB  - Fire blight disease, caused by plant pathogenic bacteriaErwinia amylovorarepresents a global threat for pome fruit industry, affecting yield and causing serious economic damage. During six year period (2011-2016), a total of 45 isolates typical forE. amylovorawere isolated from four different host plants (apple, pear, quince and medlar) in 21 localities within four Serbian regions (Southeast, Central, West, and North). Isolates were primarily identified using biochemical and serological tests, polymerase chain reaction (PCR) with species-specific primer pair (A/B) and their pathogenicity. Polyclonal antibodies used in DAS ELISA and IF were found to be specific and sensitive enough for rapidE. amylovoradetection. In virulence test performed on immature pear fruitlets, higher variability (CV) was found between regions than among hosts from which isolates originated. Slighter virulence of isolates originated from North region of Serbia (Vojvodina) was found in comparison to the isolates from Central Serbia, which were the most variable. Principal components analysis (PCA) segregated quince as a host among the most prevalent isolates in group of highly virulent, but failed to identify difference related to geographic origin of Serbian isolates. The same results enabled multivariate statistical analysis. Different DNA fingerprinting techniques such as repetitive element sequence based PCR (rep-) with BOX, ERIC, REP, GTG(5)and SERE primers, then Random Amplified Polymorphic DNA (RAPD) with M13-PCR primer revealed genetic homogeneity among all SerbianE. amylovoraisolates. ERIC, SERE and M13 primers generated the most complex and visually perceptible patters. Restriction fragment length polymorphism analysis (RFLP) ofgyrAandrecAgenes, digested with three restriction endonucleasesBstUI,BgIII andBsmI andrpoSgene digested withBstUI gave the unuque, characteristic patterns for all Serbian isolates.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates
EP  - 872
IS  - 4
SP  - 857
VL  - 157
DO  - 10.1007/s10658-020-02046-1
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Živković, Ljiljana and Živković, Natalija and Ilicic, Renata and Stanisavljević, Rade and Stanković, Slaviša",
year = "2020",
abstract = "Fire blight disease, caused by plant pathogenic bacteriaErwinia amylovorarepresents a global threat for pome fruit industry, affecting yield and causing serious economic damage. During six year period (2011-2016), a total of 45 isolates typical forE. amylovorawere isolated from four different host plants (apple, pear, quince and medlar) in 21 localities within four Serbian regions (Southeast, Central, West, and North). Isolates were primarily identified using biochemical and serological tests, polymerase chain reaction (PCR) with species-specific primer pair (A/B) and their pathogenicity. Polyclonal antibodies used in DAS ELISA and IF were found to be specific and sensitive enough for rapidE. amylovoradetection. In virulence test performed on immature pear fruitlets, higher variability (CV) was found between regions than among hosts from which isolates originated. Slighter virulence of isolates originated from North region of Serbia (Vojvodina) was found in comparison to the isolates from Central Serbia, which were the most variable. Principal components analysis (PCA) segregated quince as a host among the most prevalent isolates in group of highly virulent, but failed to identify difference related to geographic origin of Serbian isolates. The same results enabled multivariate statistical analysis. Different DNA fingerprinting techniques such as repetitive element sequence based PCR (rep-) with BOX, ERIC, REP, GTG(5)and SERE primers, then Random Amplified Polymorphic DNA (RAPD) with M13-PCR primer revealed genetic homogeneity among all SerbianE. amylovoraisolates. ERIC, SERE and M13 primers generated the most complex and visually perceptible patters. Restriction fragment length polymorphism analysis (RFLP) ofgyrAandrecAgenes, digested with three restriction endonucleasesBstUI,BgIII andBsmI andrpoSgene digested withBstUI gave the unuque, characteristic patterns for all Serbian isolates.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates",
pages = "872-857",
number = "4",
volume = "157",
doi = "10.1007/s10658-020-02046-1"
}

Popović, T., Jelušić, A., Živković, L., Živković, N., Ilicic, R., Stanisavljević, R.,& Stanković, S.. (2020). Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates. in European Journal of Plant Pathology
Springer, Dordrecht., 157(4), 857-872.
https://doi.org/10.1007/s10658-020-02046-1

Popović T, Jelušić A, Živković L, Živković N, Ilicic R, Stanisavljević R, Stanković S. Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates. in European Journal of Plant Pathology. 2020;157(4):857-872.
doi:10.1007/s10658-020-02046-1 .

Popović, Tatjana, Jelušić, Aleksandra, Živković, Ljiljana, Živković, Natalija, Ilicic, Renata, Stanisavljević, Rade, Stanković, Slaviša, "Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates" in European Journal of Plant Pathology, 157, no. 4 (2020):857-872,
https://doi.org/10.1007/s10658-020-02046-1 . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1935
AB  - Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or 
endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. 
Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight 
symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae
vegetables.
Objectives: Metagenomic approach was used to reveal differences in microbial communities 
colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial
blight symptoms and one healthy variety, all collected from five different localities in Vojvodina 
(Serbia) in 2018. 
Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA
sequences from five tested samples, and to gain insight into their alpha and beta diversity. 
Comparative taxonomic analysis of the obtained sequencing metadata was processed 
bioinformaticaly.
Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, 
Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. 
Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike 
healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the 
most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were 
present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in 
healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 
1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) 
in healthy oilseed rape variety.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape
SP  - 899
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1935
ER  - 

@conference{
author = "Jelušić, Aleksandra and Dimkić, Ivica and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or 
endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. 
Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight 
symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae
vegetables.
Objectives: Metagenomic approach was used to reveal differences in microbial communities 
colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial
blight symptoms and one healthy variety, all collected from five different localities in Vojvodina 
(Serbia) in 2018. 
Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA
sequences from five tested samples, and to gain insight into their alpha and beta diversity. 
Comparative taxonomic analysis of the obtained sequencing metadata was processed 
bioinformaticaly.
Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, 
Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. 
Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike 
healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the 
most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were 
present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in 
healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 
1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) 
in healthy oilseed rape variety.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape",
pages = "899",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1935"
}

Jelušić, A., Dimkić, I., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 899.
https://hdl.handle.net/21.15107/rcub_rimsi_1935

Jelušić A, Dimkić I, Berić T, Mitrović P, Marković S, Stanković S, Popović T. Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape. in 8th Congress of  European Microbiologists. 2019;:899.
https://hdl.handle.net/21.15107/rcub_rimsi_1935 .

Jelušić, Aleksandra, Dimkić, Ivica, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape" in 8th Congress of  European Microbiologists (2019):899,
https://hdl.handle.net/21.15107/rcub_rimsi_1935 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1933
AB  - Background: Oilseed rape (Brassica napus) is an oil-producing crop, significant for vegetable oil and 
biodiesel production. The phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) 
causing bacterial leaf blight was recorded for a first time in 2010 on self-growing winter oilseed rape 
in Serbia. Ever since, it continued appearing on cultivated crops too. 
Objectives: The aim of this research was to reveal genetic heterogeneity among 25 Xcc isolates, 
originated from different cultivars of winter oilseed rape obtained in nine-year period (2010-2018) 
from different localities in Vojvodina region (Serbia) using repetitive sequence-based PCR (rep-PCR) 
fingerprinting. 
Methods: Genomic DNA was extracted using CTAB method. Specific primer pair XCF/XCR, amplifying 
a DNA fragment of hrpF gene from X. campestris was used for rapid preliminary identification of all 
tested isolates. Rep-PCR using BOX, ERIC and REP-PCR primers was used to detect presence of 
genetic heterogeneity among Xcc isolates.
Results: All 25 tested isolates were preliminary identified as Xcc based on a positive PCR reaction 
after amplification of 525 bp region using XCF/XCR primers. BOX and ERIC-PCR generated seventeen 
different DNA fingerprinting profiles, unlike REP-PCR where only nine different profiles were 
detected. These results show presence of genetic polymorphism among Xcc isolates from winter 
oilseed rape, which could indicate either their affiliation to some of the identified Xcc races, or 
formation of a new Xcc ecotype.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques
SP  - 991
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1933
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2019",
abstract = "Background: Oilseed rape (Brassica napus) is an oil-producing crop, significant for vegetable oil and 
biodiesel production. The phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) 
causing bacterial leaf blight was recorded for a first time in 2010 on self-growing winter oilseed rape 
in Serbia. Ever since, it continued appearing on cultivated crops too. 
Objectives: The aim of this research was to reveal genetic heterogeneity among 25 Xcc isolates, 
originated from different cultivars of winter oilseed rape obtained in nine-year period (2010-2018) 
from different localities in Vojvodina region (Serbia) using repetitive sequence-based PCR (rep-PCR) 
fingerprinting. 
Methods: Genomic DNA was extracted using CTAB method. Specific primer pair XCF/XCR, amplifying 
a DNA fragment of hrpF gene from X. campestris was used for rapid preliminary identification of all 
tested isolates. Rep-PCR using BOX, ERIC and REP-PCR primers was used to detect presence of 
genetic heterogeneity among Xcc isolates.
Results: All 25 tested isolates were preliminary identified as Xcc based on a positive PCR reaction 
after amplification of 525 bp region using XCF/XCR primers. BOX and ERIC-PCR generated seventeen 
different DNA fingerprinting profiles, unlike REP-PCR where only nine different profiles were 
detected. These results show presence of genetic polymorphism among Xcc isolates from winter 
oilseed rape, which could indicate either their affiliation to some of the identified Xcc races, or 
formation of a new Xcc ecotype.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques",
pages = "991",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1933"
}

Jelušić, A., Popović, T., Mitrović, P., Dimkić, I., Stanković, S., Marković, S.,& Berić, T.. (2019). Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 991.
https://hdl.handle.net/21.15107/rcub_rimsi_1933

Jelušić A, Popović T, Mitrović P, Dimkić I, Stanković S, Marković S, Berić T. Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques. in 8th Congress of  European Microbiologists. 2019;:991.
https://hdl.handle.net/21.15107/rcub_rimsi_1933 .

Jelušić, Aleksandra, Popović, Tatjana, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques" in 8th Congress of  European Microbiologists (2019):991,
https://hdl.handle.net/21.15107/rcub_rimsi_1933 .

TY  - CONF
AU  - Marković, Sanja
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1931
AB  - Background: Pectolytic Dickeya and Pectobacterium species that cause blackleg, aerial stem rot and 
soft rot are commercially important pathogens in potato production. Blackleg symptom caused by 
these bacteria has been recorded in potato production field in Bačka region (Vojvodina, Serbia) in 
2018. 
Objectives: Metagenomic approach was used to reveal microbial communities colonizing the 
diseased potato tubers with the aim of better understanding of pathogens distribution.
Methods: Single colonies of potential pathogenic bacteria were isolated on nutrient agar and 
checked for pectolytic activity on potato slices. Ten selected bacterial isolates were identified to the 
species level after PCR amplification with gapA and mdh primers. The 16S rRNA sequences from 
diseased potato tubers samples were obtained using Next generation sequencing (NGS) Illumina 
platform. The obtained sequencing data was processed bioinformaticaly.
Results: Mixed infection of the diseased potato plants with Dickeya dianthocola and Pectobacterium 
carotovorum pv. brasiliensis was revealed after pathogen identification with gapA and mdh genes. All 
isolates caused soft rot symptoms on potato slices. Metagenomic analysis revealed that present 
bacteria belong to phylum Bacteroidetes (Bacteroides, Empedobacter, Dysgonomonas, Myroides, and 
ud-Porphyromonadaceae), Firmicutes (Vagococcus, Lactococcus, Clostridium XIVa, Enterococcus, and 
ud-Acidaminococcaceae) and Proteobacteria (Acinetobacter, ud-Pseudomonadaceae, ud Enterobacteriaceae, Wohlfahrtiimonas, ud-Neisseriaceae, Enterobacter, Providencia, Arcobacter, and 
Kerstersia) as the most common representives. Genus Pectobacterium was present in 0.5%.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Metagenomic analysis of microbial communities associated with diseased potato tubers
SP  - 881
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1931
ER  - 

@conference{
author = "Marković, Sanja and Dimkić, Ivica and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović, Tatjana",
year = "2019",
abstract = "Background: Pectolytic Dickeya and Pectobacterium species that cause blackleg, aerial stem rot and 
soft rot are commercially important pathogens in potato production. Blackleg symptom caused by 
these bacteria has been recorded in potato production field in Bačka region (Vojvodina, Serbia) in 
2018. 
Objectives: Metagenomic approach was used to reveal microbial communities colonizing the 
diseased potato tubers with the aim of better understanding of pathogens distribution.
Methods: Single colonies of potential pathogenic bacteria were isolated on nutrient agar and 
checked for pectolytic activity on potato slices. Ten selected bacterial isolates were identified to the 
species level after PCR amplification with gapA and mdh primers. The 16S rRNA sequences from 
diseased potato tubers samples were obtained using Next generation sequencing (NGS) Illumina 
platform. The obtained sequencing data was processed bioinformaticaly.
Results: Mixed infection of the diseased potato plants with Dickeya dianthocola and Pectobacterium 
carotovorum pv. brasiliensis was revealed after pathogen identification with gapA and mdh genes. All 
isolates caused soft rot symptoms on potato slices. Metagenomic analysis revealed that present 
bacteria belong to phylum Bacteroidetes (Bacteroides, Empedobacter, Dysgonomonas, Myroides, and 
ud-Porphyromonadaceae), Firmicutes (Vagococcus, Lactococcus, Clostridium XIVa, Enterococcus, and 
ud-Acidaminococcaceae) and Proteobacteria (Acinetobacter, ud-Pseudomonadaceae, ud Enterobacteriaceae, Wohlfahrtiimonas, ud-Neisseriaceae, Enterobacter, Providencia, Arcobacter, and 
Kerstersia) as the most common representives. Genus Pectobacterium was present in 0.5%.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Metagenomic analysis of microbial communities associated with diseased potato tubers",
pages = "881",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1931"
}

Marković, S., Dimkić, I., Stanković, S., Jelušić, A., Iličić, R.,& Popović, T.. (2019). Metagenomic analysis of microbial communities associated with diseased potato tubers. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 881.
https://hdl.handle.net/21.15107/rcub_rimsi_1931

Marković S, Dimkić I, Stanković S, Jelušić A, Iličić R, Popović T. Metagenomic analysis of microbial communities associated with diseased potato tubers. in 8th Congress of  European Microbiologists. 2019;:881.
https://hdl.handle.net/21.15107/rcub_rimsi_1931 .

Marković, Sanja, Dimkić, Ivica, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović, Tatjana, "Metagenomic analysis of microbial communities associated with diseased potato tubers" in 8th Congress of  European Microbiologists (2019):881,
https://hdl.handle.net/21.15107/rcub_rimsi_1931 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1912
AB  - Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant 
for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also 
for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can 
be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most 
significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) 
causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing 
bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from 
different localities in Serbia, with the aim to examine their genetic features, through 
sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified 
with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained 
sequences were checked for homology with strains available in NCBI database. Multilocus 
sequence analysis (MLSA) was then performed to determine relatedness among the tested 
isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing 
99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided
into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates
from this host. These results could be connected with pathogen adaptation to winter oilseed
rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable
crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria.
This observation on Serbian Xcc isolates gives a significant input on genetic variation and
constitutes a highly informative sample of X. campestris diversity.
PB  - Serbian Genetic Society,  Belgrade, Serbia
C3  - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
T1  - GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE
SP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1912
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant 
for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also 
for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can 
be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most 
significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) 
causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing 
bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from 
different localities in Serbia, with the aim to examine their genetic features, through 
sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified 
with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained 
sequences were checked for homology with strains available in NCBI database. Multilocus 
sequence analysis (MLSA) was then performed to determine relatedness among the tested 
isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing 
99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided
into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates
from this host. These results could be connected with pathogen adaptation to winter oilseed
rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable
crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria.
This observation on Serbian Xcc isolates gives a significant input on genetic variation and
constitutes a highly informative sample of X. campestris diversity.",
publisher = "Serbian Genetic Society,  Belgrade, Serbia",
journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY",
title = "GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE",
pages = "163",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1912"
}

Jelušić, A., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
Serbian Genetic Society,  Belgrade, Serbia., 163.
https://hdl.handle.net/21.15107/rcub_rimsi_1912

Jelušić A, Berić T, Mitrović P, Marković S, Stanković S, Popović T. GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:163.
https://hdl.handle.net/21.15107/rcub_rimsi_1912 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):163,
https://hdl.handle.net/21.15107/rcub_rimsi_1912 .

TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1915
AB  - Blackleg disease, caused by pectolytic bacteria from genus Dickeya and Pectobacterium
is currently being one of the main concerns in potato production. Yield loss and reduced 
quality, visible through potato black rotting at the stem base, as well as whole plant decaying, 
makes potato cultivation less profitable. Blackleg disease outbreak was observed in potato 
field (cv. Lady Claire) in Bačka (northern Serbia) in June 2018. The percent of the infected 
plants, estimated during the vegetation season was 45%, with yield reduction of about 20%. 
In this study the causal pathogens were genetically characterized using multilocus sequence 
analysis (MLSA). DNA of the obtained isolates was amplified with primers made based on 
the sequences of five housekeeping genes - gapA, icdA, mdh, pgi and proA and sequenced. 
Phylogenetic analysis was performed to compare the obtained isolates with the ones already 
deposited in NCBI database. NCBI BLAST identified isolates as Dickeya dianthicola and 
Pectobacterium carotovorum subsp. brasiliensis, indicating on the persistence of mix infection 
on observed potato crop. According to all genes, isolates appeared to be the most similar 
(99-100%) to the reference strains - D. dianthicola (CFBP 1200) and P. carotovorum subsp. 
brasiliensis (BC1). Blast results were confirmed after phylogenetic analysis, where isolates 
of both identified species were clustering with the rest of the strains of the same species, 
obtained from NCBI. Our findings confirm the presence of new blackleg causal agents, D. 
dianthicola and P. carotovorum subsp. brasiliensis on potato in Serbia. Their isolation and 
localization is of great importance, especially D. dianthicola which has quarantine status, and 
therefore it gives a signal for pathogen eradication and prevention of its future spreading.
PB  - Serbian Genetic Society,  Belgrade, Serbia
C3  - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
T1  - GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO
SP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1915
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2019",
abstract = "Blackleg disease, caused by pectolytic bacteria from genus Dickeya and Pectobacterium
is currently being one of the main concerns in potato production. Yield loss and reduced 
quality, visible through potato black rotting at the stem base, as well as whole plant decaying, 
makes potato cultivation less profitable. Blackleg disease outbreak was observed in potato 
field (cv. Lady Claire) in Bačka (northern Serbia) in June 2018. The percent of the infected 
plants, estimated during the vegetation season was 45%, with yield reduction of about 20%. 
In this study the causal pathogens were genetically characterized using multilocus sequence 
analysis (MLSA). DNA of the obtained isolates was amplified with primers made based on 
the sequences of five housekeeping genes - gapA, icdA, mdh, pgi and proA and sequenced. 
Phylogenetic analysis was performed to compare the obtained isolates with the ones already 
deposited in NCBI database. NCBI BLAST identified isolates as Dickeya dianthicola and 
Pectobacterium carotovorum subsp. brasiliensis, indicating on the persistence of mix infection 
on observed potato crop. According to all genes, isolates appeared to be the most similar 
(99-100%) to the reference strains - D. dianthicola (CFBP 1200) and P. carotovorum subsp. 
brasiliensis (BC1). Blast results were confirmed after phylogenetic analysis, where isolates 
of both identified species were clustering with the rest of the strains of the same species, 
obtained from NCBI. Our findings confirm the presence of new blackleg causal agents, D. 
dianthicola and P. carotovorum subsp. brasiliensis on potato in Serbia. Their isolation and 
localization is of great importance, especially D. dianthicola which has quarantine status, and 
therefore it gives a signal for pathogen eradication and prevention of its future spreading.",
publisher = "Serbian Genetic Society,  Belgrade, Serbia",
journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY",
title = "GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO",
pages = "165",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1915"
}

Marković, S., Popović, T., Jelušić, A., Iličić, R.,& Stanković, S.. (2019). GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
Serbian Genetic Society,  Belgrade, Serbia., 165.
https://hdl.handle.net/21.15107/rcub_rimsi_1915

Marković S, Popović T, Jelušić A, Iličić R, Stanković S. GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:165.
https://hdl.handle.net/21.15107/rcub_rimsi_1915 .

Marković, Sanja, Popović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):165,
https://hdl.handle.net/21.15107/rcub_rimsi_1915 .