TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1954
AB  - Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1954
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2020",
abstract = "Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1954"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Berić, T.. (2020). PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954

Jelušić A, Popović T, Dimkić I, Mitrović P, Stanković S, Marković S, Berić T. PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology. 2020;:134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS" in FEMS Online Conference on Microbiology (2020):134,
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .