TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Milosavić, Nenad
AU  - Prodanović, Radivoje
AU  - Ducic, Tanja
AU  - Radotić, Ksenija
PY  - 2007
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/233
AB  - Variations were studied of the activity and isoenzyme patterns of soluble peroxidase, catalase, catechol oxidase and superoxide dismutase, in needles of the Balkan endemic conifer Serbian spruce, Picea omorika (Panc.) Purkinye. The samples were collected from the natural habitat of the species, Mt. Tara. Seasonal changes were found to affect enzymatic activities and isoenzyme profiles. Total protein content was significantly lower in the summer than in other seasons. Several isoforms of peroxidase, catechol oxidase and superoxide dismutase (SOD), as well as two catalase isoenzymes were detected. The number of peroxidase isoenzymes was greatest during the vegetative season. Catalase and catechol oxidase peaked in summer and spring, respectively. Total SOD and Mn-SOD activities were significantly higher in the winter samples than the summer ones.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Biochemical Systematics and Ecology
T1  - Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)
EP  - 273
IS  - 5
SP  - 263
VL  - 35
DO  - 10.1016/j.bse.2006.12.001
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Milosavić, Nenad and Prodanović, Radivoje and Ducic, Tanja and Radotić, Ksenija",
year = "2007",
abstract = "Variations were studied of the activity and isoenzyme patterns of soluble peroxidase, catalase, catechol oxidase and superoxide dismutase, in needles of the Balkan endemic conifer Serbian spruce, Picea omorika (Panc.) Purkinye. The samples were collected from the natural habitat of the species, Mt. Tara. Seasonal changes were found to affect enzymatic activities and isoenzyme profiles. Total protein content was significantly lower in the summer than in other seasons. Several isoforms of peroxidase, catechol oxidase and superoxide dismutase (SOD), as well as two catalase isoenzymes were detected. The number of peroxidase isoenzymes was greatest during the vegetative season. Catalase and catechol oxidase peaked in summer and spring, respectively. Total SOD and Mn-SOD activities were significantly higher in the winter samples than the summer ones.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Biochemical Systematics and Ecology",
title = "Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)",
pages = "273-263",
number = "5",
volume = "35",
doi = "10.1016/j.bse.2006.12.001"
}

Bogdanović Pristov, J., Milosavić, N., Prodanović, R., Ducic, T.,& Radotić, K.. (2007). Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye). in Biochemical Systematics and Ecology
Pergamon-Elsevier Science Ltd, Oxford., 35(5), 263-273.
https://doi.org/10.1016/j.bse.2006.12.001

Bogdanović Pristov J, Milosavić N, Prodanović R, Ducic T, Radotić K. Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye). in Biochemical Systematics and Ecology. 2007;35(5):263-273.
doi:10.1016/j.bse.2006.12.001 .

Bogdanović Pristov, Jelena, Milosavić, Nenad, Prodanović, Radivoje, Ducic, Tanja, Radotić, Ksenija, "Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)" in Biochemical Systematics and Ecology, 35, no. 5 (2007):263-273,
https://doi.org/10.1016/j.bse.2006.12.001 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Prodanović, Radivoje
AU  - Milosavić, Nenad
AU  - Prodanović, Olivera
AU  - Radotić, Ksenija
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2527
AB  - Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Fe- isoform was not present in a detectable amount.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]
EP  - 214
IS  - 4
SP  - 211
VL  - 58
DO  - 10.2298/ABS0604211B
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Prodanović, Radivoje and Milosavić, Nenad and Prodanović, Olivera and Radotić, Ksenija",
year = "2006",
abstract = "Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Fe- isoform was not present in a detectable amount.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]",
pages = "214-211",
number = "4",
volume = "58",
doi = "10.2298/ABS0604211B"
}

Bogdanović Pristov, J., Prodanović, R., Milosavić, N., Prodanović, O.,& Radotić, K.. (2006). Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]. in Archives of Biological Sciences
Serbian Biological Society., 58(4), 211-214.
https://doi.org/10.2298/ABS0604211B

Bogdanović Pristov J, Prodanović R, Milosavić N, Prodanović O, Radotić K. Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]. in Archives of Biological Sciences. 2006;58(4):211-214.
doi:10.2298/ABS0604211B .

Bogdanović Pristov, Jelena, Prodanović, Radivoje, Milosavić, Nenad, Prodanović, Olivera, Radotić, Ksenija, "Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]" in Archives of Biological Sciences, 58, no. 4 (2006):211-214,
https://doi.org/10.2298/ABS0604211B . .

TY  - JOUR
AU  - Prodanović, Radivoje
AU  - Milosavić, Nenad
AU  - Jovanović, Slobodan
AU  - Prodanović, Olivera
AU  - Ćirković-Velicković, Tanja
AU  - Vujcic, Zoran
AU  - Jankov, Ratko M.
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/150
AB  - The activity of alpha-glucosidase from baker's yeast was determined in various concentrations of dioxan, tetrahydrofuran, tert-butanol, dimethylformamide, methanol and dimethylsulfoxide (DMSO). Higher activities were observed with sucrose than with nitrophenylglucoside as substrate in cosolvent mixtures. In 30% (v/v) DMSO, 25% of the activity obtained in pure water was detected, and in 30% (v/v) methanol 12.5% of the activity in pure water was detected, while in other cosolvents there was almost no activity under these conditions. alpha-glucosidase was immobilized onto a macroporous copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate, poly(GMA-co-EGDMA), by the glutaraldehyde method. By immobilization, the half-life of the enzyme in 35% (v/v) methanol was increased from 6 to 60min and from 4 to 15min in 45% (v/v) DMSO. The activity of the immobilized enzyme in 30% (v/v) DMSO and 30% (v/v) methanol was 22% and 18% of the activity in pure water, respectively.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Biocatalysis and Biotransformation
T1  - Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems
EP  - 200
IS  - 3
SP  - 195
VL  - 24
DO  - 10.1080/10242420600655903
ER  - 

@article{
author = "Prodanović, Radivoje and Milosavić, Nenad and Jovanović, Slobodan and Prodanović, Olivera and Ćirković-Velicković, Tanja and Vujcic, Zoran and Jankov, Ratko M.",
year = "2006",
abstract = "The activity of alpha-glucosidase from baker's yeast was determined in various concentrations of dioxan, tetrahydrofuran, tert-butanol, dimethylformamide, methanol and dimethylsulfoxide (DMSO). Higher activities were observed with sucrose than with nitrophenylglucoside as substrate in cosolvent mixtures. In 30% (v/v) DMSO, 25% of the activity obtained in pure water was detected, and in 30% (v/v) methanol 12.5% of the activity in pure water was detected, while in other cosolvents there was almost no activity under these conditions. alpha-glucosidase was immobilized onto a macroporous copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate, poly(GMA-co-EGDMA), by the glutaraldehyde method. By immobilization, the half-life of the enzyme in 35% (v/v) methanol was increased from 6 to 60min and from 4 to 15min in 45% (v/v) DMSO. The activity of the immobilized enzyme in 30% (v/v) DMSO and 30% (v/v) methanol was 22% and 18% of the activity in pure water, respectively.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Biocatalysis and Biotransformation",
title = "Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems",
pages = "200-195",
number = "3",
volume = "24",
doi = "10.1080/10242420600655903"
}

Prodanović, R., Milosavić, N., Jovanović, S., Prodanović, O., Ćirković-Velicković, T., Vujcic, Z.,& Jankov, R. M.. (2006). Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems. in Biocatalysis and Biotransformation
Taylor & Francis Ltd, Abingdon., 24(3), 195-200.
https://doi.org/10.1080/10242420600655903

Prodanović R, Milosavić N, Jovanović S, Prodanović O, Ćirković-Velicković T, Vujcic Z, Jankov RM. Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems. in Biocatalysis and Biotransformation. 2006;24(3):195-200.
doi:10.1080/10242420600655903 .

Prodanović, Radivoje, Milosavić, Nenad, Jovanović, Slobodan, Prodanović, Olivera, Ćirković-Velicković, Tanja, Vujcic, Zoran, Jankov, Ratko M., "Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems" in Biocatalysis and Biotransformation, 24, no. 3 (2006):195-200,
https://doi.org/10.1080/10242420600655903 . .

TY  - CONF
AU  - Bogdanović Pristov, Jelena
AU  - Prodanović, Radivoje
AU  - Milosavić, Nenad
AU  - Prodanović, Olivera
AU  - Radotić, Ksenija
PY  - 2004
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2662
AB  - Activity and isoenzyme composition or superoxide dismutase (EC 1.15.1.1) in apoplast and whole-needle extract of P. omorika was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic (pl 3-6) and two slow-migrating basic (pl 7.5-8) SOD isoforms have been found in the whole extract. The extracellular SOD had an extremely acidic (pI 3) isoform. Using specific inhibitors, Cu/Zn- and Mn- superoxide dismutase forms were identified in the total extract, while in the apoplast only Mn- SOD was observed. Fe-isoform was not present in a detectable level. Presented results are important, since superoxide dismutase is involved in plant response to the increased level of organic and oxygen free radicals and related H202 increase. It is also employed as parameter in biomonitoring of the environment. This work is the first study of superoxide dismutase in omorika species.
PB  - Yugoslav Biophysical Society
C3  - 22nd International Symposium on Biophysics
T1  - MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST  AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye)
SP  - S2,P8
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2662
ER  - 

@conference{
author = "Bogdanović Pristov, Jelena and Prodanović, Radivoje and Milosavić, Nenad and Prodanović, Olivera and Radotić, Ksenija",
year = "2004",
abstract = "Activity and isoenzyme composition or superoxide dismutase (EC 1.15.1.1) in apoplast and whole-needle extract of P. omorika was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic (pl 3-6) and two slow-migrating basic (pl 7.5-8) SOD isoforms have been found in the whole extract. The extracellular SOD had an extremely acidic (pI 3) isoform. Using specific inhibitors, Cu/Zn- and Mn- superoxide dismutase forms were identified in the total extract, while in the apoplast only Mn- SOD was observed. Fe-isoform was not present in a detectable level. Presented results are important, since superoxide dismutase is involved in plant response to the increased level of organic and oxygen free radicals and related H202 increase. It is also employed as parameter in biomonitoring of the environment. This work is the first study of superoxide dismutase in omorika species.",
publisher = "Yugoslav Biophysical Society",
journal = "22nd International Symposium on Biophysics",
title = "MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST  AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye)",
pages = "S2,P8",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2662"
}

Bogdanović Pristov, J., Prodanović, R., Milosavić, N., Prodanović, O.,& Radotić, K.. (2004). MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST  AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye). in 22nd International Symposium on Biophysics
Yugoslav Biophysical Society., S2,P8.
https://hdl.handle.net/21.15107/rcub_rimsi_2662

Bogdanović Pristov J, Prodanović R, Milosavić N, Prodanović O, Radotić K. MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST  AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye). in 22nd International Symposium on Biophysics. 2004;:S2,P8.
https://hdl.handle.net/21.15107/rcub_rimsi_2662 .

Bogdanović Pristov, Jelena, Prodanović, Radivoje, Milosavić, Nenad, Prodanović, Olivera, Radotić, Ksenija, "MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST  AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye)" in 22nd International Symposium on Biophysics (2004):S2,P8,
https://hdl.handle.net/21.15107/rcub_rimsi_2662 .