MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye)
Аутори
Bogdanović Pristov, JelenaProdanović, Radivoje
Milosavić, Nenad
Prodanović, Olivera
Radotić, Ksenija
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Activity and isoenzyme composition or superoxide dismutase (EC 1.15.1.1) in apoplast and whole-needle extract of P. omorika was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic (pl 3-6) and two slow-migrating basic (pl 7.5-8) SOD isoforms have been found in the whole extract. The extracellular SOD had an extremely acidic (pI 3) isoform. Using specific inhibitors, Cu/Zn- and Mn- superoxide dismutase forms were identified in the total extract, while in the apoplast only Mn- SOD was observed. Fe-isoform was not present in a detectable level. Presented results are important, since superoxide dismutase is involved in plant response to the increased level of organic and oxygen free radicals and related H202 increase. It is also employed as parameter in biomonitoring of the environment. This work is the first study of superoxide dismutase in omorika species.
Кључне речи:
superoxide dismutase / Picea omorika / isoenzyme compositionИзвор:
22nd International Symposium on Biophysics, 2004, S2,P8-Издавач:
- Yugoslav Biophysical Society
Финансирање / пројекти:
- Испитивања нових биосензора за мониторинг и дијагностику биљака (RS-MESTD-MPN2006-2010-143043)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Bogdanović Pristov, Jelena AU - Prodanović, Radivoje AU - Milosavić, Nenad AU - Prodanović, Olivera AU - Radotić, Ksenija PY - 2004 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2662 AB - Activity and isoenzyme composition or superoxide dismutase (EC 1.15.1.1) in apoplast and whole-needle extract of P. omorika was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic (pl 3-6) and two slow-migrating basic (pl 7.5-8) SOD isoforms have been found in the whole extract. The extracellular SOD had an extremely acidic (pI 3) isoform. Using specific inhibitors, Cu/Zn- and Mn- superoxide dismutase forms were identified in the total extract, while in the apoplast only Mn- SOD was observed. Fe-isoform was not present in a detectable level. Presented results are important, since superoxide dismutase is involved in plant response to the increased level of organic and oxygen free radicals and related H202 increase. It is also employed as parameter in biomonitoring of the environment. This work is the first study of superoxide dismutase in omorika species. PB - Yugoslav Biophysical Society C3 - 22nd International Symposium on Biophysics T1 - MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye) SP - S2,P8 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2662 ER -
@conference{ author = "Bogdanović Pristov, Jelena and Prodanović, Radivoje and Milosavić, Nenad and Prodanović, Olivera and Radotić, Ksenija", year = "2004", abstract = "Activity and isoenzyme composition or superoxide dismutase (EC 1.15.1.1) in apoplast and whole-needle extract of P. omorika was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic (pl 3-6) and two slow-migrating basic (pl 7.5-8) SOD isoforms have been found in the whole extract. The extracellular SOD had an extremely acidic (pI 3) isoform. Using specific inhibitors, Cu/Zn- and Mn- superoxide dismutase forms were identified in the total extract, while in the apoplast only Mn- SOD was observed. Fe-isoform was not present in a detectable level. Presented results are important, since superoxide dismutase is involved in plant response to the increased level of organic and oxygen free radicals and related H202 increase. It is also employed as parameter in biomonitoring of the environment. This work is the first study of superoxide dismutase in omorika species.", publisher = "Yugoslav Biophysical Society", journal = "22nd International Symposium on Biophysics", title = "MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye)", pages = "S2,P8", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2662" }
Bogdanović Pristov, J., Prodanović, R., Milosavić, N., Prodanović, O.,& Radotić, K.. (2004). MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye). in 22nd International Symposium on Biophysics Yugoslav Biophysical Society., S2,P8. https://hdl.handle.net/21.15107/rcub_rimsi_2662
Bogdanović Pristov J, Prodanović R, Milosavić N, Prodanović O, Radotić K. MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye). in 22nd International Symposium on Biophysics. 2004;:S2,P8. https://hdl.handle.net/21.15107/rcub_rimsi_2662 .
Bogdanović Pristov, Jelena, Prodanović, Radivoje, Milosavić, Nenad, Prodanović, Olivera, Radotić, Ksenija, "MULTIPLE FORMS OF SUPEROXIDE DISMUTASE IN APOPLAST AND WHOLE-NEEDLE EXTRACT OF OMORIKA (Picea omorika (PANC.) purkinye)" in 22nd International Symposium on Biophysics (2004):S2,P8, https://hdl.handle.net/21.15107/rcub_rimsi_2662 .