TY  - JOUR
AU  - Hadzibrahimović, Mirzeta
AU  - Sužnjević, Desanka
AU  - Pastor, Ferenc
AU  - Cvetić-Antić, Tijana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Zivić, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1064
AB  - The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.
PB  - Springer, Dordrecht
T2  - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1  - The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?
EP  - 373
IS  - 3
SP  - 365
VL  - 110
DO  - 10.1007/s10482-016-0808-0
ER  - 

@article{
author = "Hadzibrahimović, Mirzeta and Sužnjević, Desanka and Pastor, Ferenc and Cvetić-Antić, Tijana and Žižić, Milan and Zakrzewska, Joanna and Zivić, Miroslav",
year = "2017",
abstract = "The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?",
pages = "373-365",
number = "3",
volume = "110",
doi = "10.1007/s10482-016-0808-0"
}

Hadzibrahimović, M., Sužnjević, D., Pastor, F., Cvetić-Antić, T., Žižić, M., Zakrzewska, J.,& Zivić, M.. (2017). The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 110(3), 365-373.
https://doi.org/10.1007/s10482-016-0808-0

Hadzibrahimović M, Sužnjević D, Pastor F, Cvetić-Antić T, Žižić M, Zakrzewska J, Zivić M. The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2017;110(3):365-373.
doi:10.1007/s10482-016-0808-0 .

Hadzibrahimović, Mirzeta, Sužnjević, Desanka, Pastor, Ferenc, Cvetić-Antić, Tijana, Žižić, Milan, Zakrzewska, Joanna, Zivić, Miroslav, "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 110, no. 3 (2017):365-373,
https://doi.org/10.1007/s10482-016-0808-0 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Miladinović, Zoran P.
AU  - Stanić, Marina
AU  - Hadzibrahimović, Mirzeta
AU  - Zivić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/986
AB  - V-51 NMR spectroscopy was used for detection and identification of cell-associated vanadate (V5+) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal were observed about 40 min after treatment. Simultaneously, a signal at -532 ppm, with increasing intensity, was detected in spectra. The time-dependent rise in this signal was connected to a decrease in the extracellular monomer signal, indicating its transport into the cell. The signal at -532 ppm did not belong to any known simple oxido-vanadate species, nor to a complex with any of the components of experimental medium. This signal was the only one present in spectrum of the mycelium washed 35 min after treatment, and the only one observed in mycelium cultivated on vanadate-contained medium. Therefore, its appearance can be attributed to intracellular complexation, and may represent an important detoxification mechanism of the cell exposed to a physiologically relevant concentration of vanadate. Experiments (V-51 NMR and polarography) performed with Cd-pretreated mycelium (inhibitor of an enzyme responsible for V5+ reduction) and ferricyanide-preincubated mycelium excluded the possibility of V5+ tetramer's entry into the cell.
PB  - Elsevier Science Bv, Amsterdam
T2  - Research in Microbiology
T1  - V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium
EP  - 528
IS  - 6
SP  - 521
VL  - 167
DO  - 10.1016/j.resmic.2016.04.012
ER  - 

@article{
author = "Žižić, Milan and Miladinović, Zoran P. and Stanić, Marina and Hadzibrahimović, Mirzeta and Zivić, Miroslav and Zakrzewska, Joanna",
year = "2016",
abstract = "V-51 NMR spectroscopy was used for detection and identification of cell-associated vanadate (V5+) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal were observed about 40 min after treatment. Simultaneously, a signal at -532 ppm, with increasing intensity, was detected in spectra. The time-dependent rise in this signal was connected to a decrease in the extracellular monomer signal, indicating its transport into the cell. The signal at -532 ppm did not belong to any known simple oxido-vanadate species, nor to a complex with any of the components of experimental medium. This signal was the only one present in spectrum of the mycelium washed 35 min after treatment, and the only one observed in mycelium cultivated on vanadate-contained medium. Therefore, its appearance can be attributed to intracellular complexation, and may represent an important detoxification mechanism of the cell exposed to a physiologically relevant concentration of vanadate. Experiments (V-51 NMR and polarography) performed with Cd-pretreated mycelium (inhibitor of an enzyme responsible for V5+ reduction) and ferricyanide-preincubated mycelium excluded the possibility of V5+ tetramer's entry into the cell.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Research in Microbiology",
title = "V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium",
pages = "528-521",
number = "6",
volume = "167",
doi = "10.1016/j.resmic.2016.04.012"
}

Žižić, M., Miladinović, Z. P., Stanić, M., Hadzibrahimović, M., Zivić, M.,& Zakrzewska, J.. (2016). V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium. in Research in Microbiology
Elsevier Science Bv, Amsterdam., 167(6), 521-528.
https://doi.org/10.1016/j.resmic.2016.04.012

Žižić M, Miladinović ZP, Stanić M, Hadzibrahimović M, Zivić M, Zakrzewska J. V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium. in Research in Microbiology. 2016;167(6):521-528.
doi:10.1016/j.resmic.2016.04.012 .

Žižić, Milan, Miladinović, Zoran P., Stanić, Marina, Hadzibrahimović, Mirzeta, Zivić, Miroslav, Zakrzewska, Joanna, "V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium" in Research in Microbiology, 167, no. 6 (2016):521-528,
https://doi.org/10.1016/j.resmic.2016.04.012 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Zivić, Miroslav
AU  - Hadzibrahimović, Mirzeta
AU  - Pajdic, Aleksandra
AU  - Krizak, Strahinja
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/809
AB  - The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus
EP  - 857
IS  - 2
SP  - 847
VL  - 66
DO  - 10.2298/ABS1402847S
ER  - 

@article{
author = "Stanić, Marina and Zivić, Miroslav and Hadzibrahimović, Mirzeta and Pajdic, Aleksandra and Krizak, Strahinja and Žižić, Milan and Zakrzewska, Joanna",
year = "2014",
abstract = "The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus",
pages = "857-847",
number = "2",
volume = "66",
doi = "10.2298/ABS1402847S"
}

Stanić, M., Zivić, M., Hadzibrahimović, M., Pajdic, A., Krizak, S., Žižić, M.,& Zakrzewska, J.. (2014). Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(2), 847-857.
https://doi.org/10.2298/ABS1402847S

Stanić M, Zivić M, Hadzibrahimović M, Pajdic A, Krizak S, Žižić M, Zakrzewska J. Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences. 2014;66(2):847-857.
doi:10.2298/ABS1402847S .

Stanić, Marina, Zivić, Miroslav, Hadzibrahimović, Mirzeta, Pajdic, Aleksandra, Krizak, Strahinja, Žižić, Milan, Zakrzewska, Joanna, "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus" in Archives of Biological Sciences, 66, no. 2 (2014):847-857,
https://doi.org/10.2298/ABS1402847S . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Zakrzewska, Joanna
AU  - Hadzibrahimović, Mirzeta R
AU  - Žižić, Milan
AU  - Marković, Zoran S
AU  - Vučinić, Željko
AU  - Zivić, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/658
AB  - Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism
EP  - 778
IS  - 7
SP  - 770
VL  - 164
DO  - 10.1016/j.resmic.2013.03.002
ER  - 

@article{
author = "Stanić, Marina and Zakrzewska, Joanna and Hadzibrahimović, Mirzeta R and Žižić, Milan and Marković, Zoran S and Vučinić, Željko and Zivić, Miroslav",
year = "2013",
abstract = "Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism",
pages = "778-770",
number = "7",
volume = "164",
doi = "10.1016/j.resmic.2013.03.002"
}

Stanić, M., Zakrzewska, J., Hadzibrahimović, M. R., Žižić, M., Marković, Z. S., Vučinić, Ž.,& Zivić, M.. (2013). Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology
Elsevier, Amsterdam., 164(7), 770-778.
https://doi.org/10.1016/j.resmic.2013.03.002

Stanić M, Zakrzewska J, Hadzibrahimović MR, Žižić M, Marković ZS, Vučinić Ž, Zivić M. Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology. 2013;164(7):770-778.
doi:10.1016/j.resmic.2013.03.002 .

Stanić, Marina, Zakrzewska, Joanna, Hadzibrahimović, Mirzeta R, Žižić, Milan, Marković, Zoran S, Vučinić, Željko, Zivić, Miroslav, "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism" in Research in Microbiology, 164, no. 7 (2013):770-778,
https://doi.org/10.1016/j.resmic.2013.03.002 . .

TY  - GEN
AU  - Stanić, Marina
AU  - Zivić, Miroslav
AU  - Hadzibrahimović, Mirzeta R
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/497
AB  - Alongside classical, cytochrome respiratory pathway, Phycomyces
blakesleeanus possess alternative, cyanideresistant
respiration (CRR) facilitated by alternative oxidase
(AOX). In order to study role of oxygen in regulation of CRR,
the effects of cyanide on respiration of 24h old mycelia in
aerated (control), hypoxic and anoxic conditions were measured.
Mycelium was incubated in these conditions for 1.5h,
3h and 5h. After 1.5h, AOX activity was increased only in
specimens incubated in anoxic conditions (13.6%). After 3h,
increase in AOX activity was significant in both hypoxic and
anoxic specimens (18.9% and 18.8%, respectively), with
even greater increase after 5h, 20.7% for hypoxic and 23.3%
for specimens in anoxic conditions. Mycelia treated for 5h
was then oxygenated for 10 minutes. This induced decrease
in AOX activity of 17% in anoxic and even 23.9% in hypoxic
mycelia. AOX is recognized as one of the mechanisms for
maintaining low levels of reduced ubiquione which can
function in conditions in which cytochrome chain is disabled,
such as anoxia. This is in concordance with results obtained
on P. blakesleeanus, where AOX levels rise in hypoxic and
anoxic conditions and decrease close to control level shortly
after introduction of oxygen into the system.
PB  - Springer, New York
T2  - European Biophysics Journal With Biophysics Letters
T1  - Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus
EP  - 186
SP  - 186
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_497
ER  - 

@misc{
author = "Stanić, Marina and Zivić, Miroslav and Hadzibrahimović, Mirzeta R and Žižić, Milan and Zakrzewska, Joanna",
year = "2011",
abstract = "Alongside classical, cytochrome respiratory pathway, Phycomyces
blakesleeanus possess alternative, cyanideresistant
respiration (CRR) facilitated by alternative oxidase
(AOX). In order to study role of oxygen in regulation of CRR,
the effects of cyanide on respiration of 24h old mycelia in
aerated (control), hypoxic and anoxic conditions were measured.
Mycelium was incubated in these conditions for 1.5h,
3h and 5h. After 1.5h, AOX activity was increased only in
specimens incubated in anoxic conditions (13.6%). After 3h,
increase in AOX activity was significant in both hypoxic and
anoxic specimens (18.9% and 18.8%, respectively), with
even greater increase after 5h, 20.7% for hypoxic and 23.3%
for specimens in anoxic conditions. Mycelia treated for 5h
was then oxygenated for 10 minutes. This induced decrease
in AOX activity of 17% in anoxic and even 23.9% in hypoxic
mycelia. AOX is recognized as one of the mechanisms for
maintaining low levels of reduced ubiquione which can
function in conditions in which cytochrome chain is disabled,
such as anoxia. This is in concordance with results obtained
on P. blakesleeanus, where AOX levels rise in hypoxic and
anoxic conditions and decrease close to control level shortly
after introduction of oxygen into the system.",
publisher = "Springer, New York",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus",
pages = "186-186",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_497"
}

Stanić, M., Zivić, M., Hadzibrahimović, M. R., Žižić, M.,& Zakrzewska, J.. (2011). Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus. in European Biophysics Journal With Biophysics Letters
Springer, New York., 40, 186-186.
https://hdl.handle.net/21.15107/rcub_rimsi_497

Stanić M, Zivić M, Hadzibrahimović MR, Žižić M, Zakrzewska J. Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus. in European Biophysics Journal With Biophysics Letters. 2011;40:186-186.
https://hdl.handle.net/21.15107/rcub_rimsi_497 .

Stanić, Marina, Zivić, Miroslav, Hadzibrahimović, Mirzeta R, Žižić, Milan, Zakrzewska, Joanna, "Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus" in European Biophysics Journal With Biophysics Letters, 40 (2011):186-186,
https://hdl.handle.net/21.15107/rcub_rimsi_497 .