TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Janakiev, Tamara
AU  - Unković, Nikola
AU  - Ljaljević Grbić, Milica
AU  - Degrassi, Giuliano
AU  - Dimkić, Ivica
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3205
AB  - The soybean is recognized worldwide as one of the most important crops due to its protein- and
oil-rich seeds. Several beneficial bacterial strains, including those of the genus Pseudomonas, are
known to increase plant yield and disease tolerance. The aim of this study was to test the potential of 15 strains of Pseudomonas spp. to promote soybean growth under field conditions. Strains were identified based on 16S rRNA and gyrB sequences, and strains belonging to risk group 1 were further analyzed. Selected strains were tested for plant growth-promoting (PGP) properties, biofilm formation and in vitro antimicrobial activity against various fungal pathogens. PGP activity indicators of the selected strains were evaluated on soybean plants grown in fertilizer-free soil and in soil treated with 70% and 100% fertilizers. Three Pseudomonas spp. strains, P. putida VB131A, P. fulva VB134B and P. rhodesiae VB143S, were selected as PGP candidates. All three strains showed the ability to produce IAA and solubilize phosphorus, while only P. rhodesiae VB143S showed strong lipase and protease activity. Only P. fulva VB134B showed the ability to
produce biofilm and hydrogen cyanide. All three strains showed in vitro antifungal activity against
Alternaria infectoria. P. fulva VB134B and P. rhodesiae VB143S showed antifungal activity against
Alternaria alternata and P. putida VB131A and P. rhodesiae VB143S against Epicoccum nigrum. Only P. fulva VB134B showed activity against Monilinia laxa. Statistically significant differences in
plant height were not observed regardless of the strain or soil treatment used. In terms of first
pod height, the P. fulva VB134B treatment was most effective when applied to soybeans grown
in 100% fertilized soil. In contrast, plants treated with P. rhodesiae VB143S and grown in 100% fertilized soil had the highest number of branches and pods per plant.
PB  - Serbian Society for Microbiology
C3  - XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"
T1  - Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions
EP  - 70
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3205
ER  - 

@conference{
author = "Jelušić, Aleksandra and Janakiev, Tamara and Unković, Nikola and Ljaljević Grbić, Milica and Degrassi, Giuliano and Dimkić, Ivica",
year = "2024",
abstract = "The soybean is recognized worldwide as one of the most important crops due to its protein- and
oil-rich seeds. Several beneficial bacterial strains, including those of the genus Pseudomonas, are
known to increase plant yield and disease tolerance. The aim of this study was to test the potential of 15 strains of Pseudomonas spp. to promote soybean growth under field conditions. Strains were identified based on 16S rRNA and gyrB sequences, and strains belonging to risk group 1 were further analyzed. Selected strains were tested for plant growth-promoting (PGP) properties, biofilm formation and in vitro antimicrobial activity against various fungal pathogens. PGP activity indicators of the selected strains were evaluated on soybean plants grown in fertilizer-free soil and in soil treated with 70% and 100% fertilizers. Three Pseudomonas spp. strains, P. putida VB131A, P. fulva VB134B and P. rhodesiae VB143S, were selected as PGP candidates. All three strains showed the ability to produce IAA and solubilize phosphorus, while only P. rhodesiae VB143S showed strong lipase and protease activity. Only P. fulva VB134B showed the ability to
produce biofilm and hydrogen cyanide. All three strains showed in vitro antifungal activity against
Alternaria infectoria. P. fulva VB134B and P. rhodesiae VB143S showed antifungal activity against
Alternaria alternata and P. putida VB131A and P. rhodesiae VB143S against Epicoccum nigrum. Only P. fulva VB134B showed activity against Monilinia laxa. Statistically significant differences in
plant height were not observed regardless of the strain or soil treatment used. In terms of first
pod height, the P. fulva VB134B treatment was most effective when applied to soybeans grown
in 100% fertilized soil. In contrast, plants treated with P. rhodesiae VB143S and grown in 100% fertilized soil had the highest number of branches and pods per plant.",
publisher = "Serbian Society for Microbiology",
journal = "XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"",
title = "Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3205"
}

Jelušić, A., Janakiev, T., Unković, N., Ljaljević Grbić, M., Degrassi, G.,& Dimkić, I.. (2024). Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions. in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"
Serbian Society for Microbiology., 70-70.
https://hdl.handle.net/21.15107/rcub_rimsi_3205

Jelušić A, Janakiev T, Unković N, Ljaljević Grbić M, Degrassi G, Dimkić I. Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions. in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health". 2024;:70-70.
https://hdl.handle.net/21.15107/rcub_rimsi_3205 .

Jelušić, Aleksandra, Janakiev, Tamara, Unković, Nikola, Ljaljević Grbić, Milica, Degrassi, Giuliano, Dimkić, Ivica, "Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions" in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health" (2024):70-70,
https://hdl.handle.net/21.15107/rcub_rimsi_3205 .

TY  - CONF
AU  - Janakiev, Tamara
AU  - Jelušić, Aleksandra
AU  - Kuzmanović, Nemanja
AU  - Fira, Đorđe
AU  - Dimkić, Ivica
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2048
AB  - BACKGROUND
Atypical Rhizobium sp. were identified as a causative agent of crown gall on rhododendron and blueberry in Germany. Considering the lack of efficient measures against crown gall disease, evaluation of innovative biocontrol measures would greatly contribute to sustainable agriculture.
OBJECTIVES
Identification of candidate bacterial strains that could be employed for biological control of the novel crown gall bacteria belonging to the ″tumorigenes″ clade.
METHODS
Antimicrobial activity of six antagonistic Pseudomonas and Bacillus strains were tested in vitro and in vivo against pathogenic strain Rhizobium sp. rho-6.2. The in vivo experiment, using co-inoculation and preventive inoculation strategies, was performed in controlled greenhouse conditions on tomato plants as a model system in four replicas and randomized.
RESULTS
The whole cultures of two antagonistic Pseudomonas strains were the most efficient against pathogenic Rhizobium sp. rho-6.2 in co-inoculation strategy with reducing tumor size of 92.86%. Contrary, in the preventive treatment same Pseudomonas strains were less efficient (15.38 and 30.77%). Despite the high in vitro antimicrobial activity of Bacillus strains their in vivo activity was less pronounced in preventive treatment (up to 15.38%), while in co-inoculation strategy was more prominent (42.86%). Consortium based on Bacillus and Pseudomonas strains, was increased biocontrol activity up to 38.6% of tumor’s reduction. The selected Pseudomonas strains
could be further tested as an alternative strategy for the biocontrol of crown gall disease and potential involvement of quorum quenching mechanism will be determined.
PB  - Federation of European Microbiological Societies
C3  - FEMS Conference on Microbiology, Belgrade, Serbia
T1  - Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease
EP  - 901
SP  - 900
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2048
ER  - 

@conference{
author = "Janakiev, Tamara and Jelušić, Aleksandra and Kuzmanović, Nemanja and Fira, Đorđe and Dimkić, Ivica",
year = "2022",
abstract = "BACKGROUND
Atypical Rhizobium sp. were identified as a causative agent of crown gall on rhododendron and blueberry in Germany. Considering the lack of efficient measures against crown gall disease, evaluation of innovative biocontrol measures would greatly contribute to sustainable agriculture.
OBJECTIVES
Identification of candidate bacterial strains that could be employed for biological control of the novel crown gall bacteria belonging to the ″tumorigenes″ clade.
METHODS
Antimicrobial activity of six antagonistic Pseudomonas and Bacillus strains were tested in vitro and in vivo against pathogenic strain Rhizobium sp. rho-6.2. The in vivo experiment, using co-inoculation and preventive inoculation strategies, was performed in controlled greenhouse conditions on tomato plants as a model system in four replicas and randomized.
RESULTS
The whole cultures of two antagonistic Pseudomonas strains were the most efficient against pathogenic Rhizobium sp. rho-6.2 in co-inoculation strategy with reducing tumor size of 92.86%. Contrary, in the preventive treatment same Pseudomonas strains were less efficient (15.38 and 30.77%). Despite the high in vitro antimicrobial activity of Bacillus strains their in vivo activity was less pronounced in preventive treatment (up to 15.38%), while in co-inoculation strategy was more prominent (42.86%). Consortium based on Bacillus and Pseudomonas strains, was increased biocontrol activity up to 38.6% of tumor’s reduction. The selected Pseudomonas strains
could be further tested as an alternative strategy for the biocontrol of crown gall disease and potential involvement of quorum quenching mechanism will be determined.",
publisher = "Federation of European Microbiological Societies",
journal = "FEMS Conference on Microbiology, Belgrade, Serbia",
title = "Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease",
pages = "901-900",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2048"
}

Janakiev, T., Jelušić, A., Kuzmanović, N., Fira, Đ.,& Dimkić, I.. (2022). Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease. in FEMS Conference on Microbiology, Belgrade, Serbia
Federation of European Microbiological Societies., 900-901.
https://hdl.handle.net/21.15107/rcub_rimsi_2048

Janakiev T, Jelušić A, Kuzmanović N, Fira Đ, Dimkić I. Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease. in FEMS Conference on Microbiology, Belgrade, Serbia. 2022;:900-901.
https://hdl.handle.net/21.15107/rcub_rimsi_2048 .

Janakiev, Tamara, Jelušić, Aleksandra, Kuzmanović, Nemanja, Fira, Đorđe, Dimkić, Ivica, "Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease" in FEMS Conference on Microbiology, Belgrade, Serbia (2022):900-901,
https://hdl.handle.net/21.15107/rcub_rimsi_2048 .