Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease
Апстракт
BACKGROUND
Atypical Rhizobium sp. were identified as a causative agent of crown gall on rhododendron and blueberry in Germany. Considering the lack of efficient measures against crown gall disease, evaluation of innovative biocontrol measures would greatly contribute to sustainable agriculture.
OBJECTIVES
Identification of candidate bacterial strains that could be employed for biological control of the novel crown gall bacteria belonging to the ″tumorigenes″ clade.
METHODS
Antimicrobial activity of six antagonistic Pseudomonas and Bacillus strains were tested in vitro and in vivo against pathogenic strain Rhizobium sp. rho-6.2. The in vivo experiment, using co-inoculation and preventive inoculation strategies, was performed in controlled greenhouse conditions on tomato plants as a model system in four replicas and randomized.
RESULTS
The whole cultures of two antagonistic Pseudomonas strains were the most efficient against pathogenic Rhizobium sp. rho-6.2 in co-inoculation strat...egy with reducing tumor size of 92.86%. Contrary, in the preventive treatment same Pseudomonas strains were less efficient (15.38 and 30.77%). Despite the high in vitro antimicrobial activity of Bacillus strains their in vivo activity was less pronounced in preventive treatment (up to 15.38%), while in co-inoculation strategy was more prominent (42.86%). Consortium based on Bacillus and Pseudomonas strains, was increased biocontrol activity up to 38.6% of tumor’s reduction. The selected Pseudomonas strains
could be further tested as an alternative strategy for the biocontrol of crown gall disease and potential involvement of quorum quenching mechanism will be determined.
Кључне речи:
crown gall / Rhizobium sp. / biological control / Bacillus / PseudomonasИзвор:
FEMS Conference on Microbiology, Belgrade, Serbia, 2022, 900-901Издавач:
- Federation of European Microbiological Societies
Финансирање / пројекти:
- This work was supported by the Serbian Science Cooperation Program with the Diaspora: Knowledge Sharing Vouchers of the Science Fund of the Republic of Serbia [Contract No. BIOCTA 6436066]
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Janakiev, Tamara AU - Jelušić, Aleksandra AU - Kuzmanović, Nemanja AU - Fira, Đorđe AU - Dimkić, Ivica PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2048 AB - BACKGROUND Atypical Rhizobium sp. were identified as a causative agent of crown gall on rhododendron and blueberry in Germany. Considering the lack of efficient measures against crown gall disease, evaluation of innovative biocontrol measures would greatly contribute to sustainable agriculture. OBJECTIVES Identification of candidate bacterial strains that could be employed for biological control of the novel crown gall bacteria belonging to the ″tumorigenes″ clade. METHODS Antimicrobial activity of six antagonistic Pseudomonas and Bacillus strains were tested in vitro and in vivo against pathogenic strain Rhizobium sp. rho-6.2. The in vivo experiment, using co-inoculation and preventive inoculation strategies, was performed in controlled greenhouse conditions on tomato plants as a model system in four replicas and randomized. RESULTS The whole cultures of two antagonistic Pseudomonas strains were the most efficient against pathogenic Rhizobium sp. rho-6.2 in co-inoculation strategy with reducing tumor size of 92.86%. Contrary, in the preventive treatment same Pseudomonas strains were less efficient (15.38 and 30.77%). Despite the high in vitro antimicrobial activity of Bacillus strains their in vivo activity was less pronounced in preventive treatment (up to 15.38%), while in co-inoculation strategy was more prominent (42.86%). Consortium based on Bacillus and Pseudomonas strains, was increased biocontrol activity up to 38.6% of tumor’s reduction. The selected Pseudomonas strains could be further tested as an alternative strategy for the biocontrol of crown gall disease and potential involvement of quorum quenching mechanism will be determined. PB - Federation of European Microbiological Societies C3 - FEMS Conference on Microbiology, Belgrade, Serbia T1 - Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease EP - 901 SP - 900 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2048 ER -
@conference{ author = "Janakiev, Tamara and Jelušić, Aleksandra and Kuzmanović, Nemanja and Fira, Đorđe and Dimkić, Ivica", year = "2022", abstract = "BACKGROUND Atypical Rhizobium sp. were identified as a causative agent of crown gall on rhododendron and blueberry in Germany. Considering the lack of efficient measures against crown gall disease, evaluation of innovative biocontrol measures would greatly contribute to sustainable agriculture. OBJECTIVES Identification of candidate bacterial strains that could be employed for biological control of the novel crown gall bacteria belonging to the ″tumorigenes″ clade. METHODS Antimicrobial activity of six antagonistic Pseudomonas and Bacillus strains were tested in vitro and in vivo against pathogenic strain Rhizobium sp. rho-6.2. The in vivo experiment, using co-inoculation and preventive inoculation strategies, was performed in controlled greenhouse conditions on tomato plants as a model system in four replicas and randomized. RESULTS The whole cultures of two antagonistic Pseudomonas strains were the most efficient against pathogenic Rhizobium sp. rho-6.2 in co-inoculation strategy with reducing tumor size of 92.86%. Contrary, in the preventive treatment same Pseudomonas strains were less efficient (15.38 and 30.77%). Despite the high in vitro antimicrobial activity of Bacillus strains their in vivo activity was less pronounced in preventive treatment (up to 15.38%), while in co-inoculation strategy was more prominent (42.86%). Consortium based on Bacillus and Pseudomonas strains, was increased biocontrol activity up to 38.6% of tumor’s reduction. The selected Pseudomonas strains could be further tested as an alternative strategy for the biocontrol of crown gall disease and potential involvement of quorum quenching mechanism will be determined.", publisher = "Federation of European Microbiological Societies", journal = "FEMS Conference on Microbiology, Belgrade, Serbia", title = "Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease", pages = "901-900", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2048" }
Janakiev, T., Jelušić, A., Kuzmanović, N., Fira, Đ.,& Dimkić, I.. (2022). Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease. in FEMS Conference on Microbiology, Belgrade, Serbia Federation of European Microbiological Societies., 900-901. https://hdl.handle.net/21.15107/rcub_rimsi_2048
Janakiev T, Jelušić A, Kuzmanović N, Fira Đ, Dimkić I. Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease. in FEMS Conference on Microbiology, Belgrade, Serbia. 2022;:900-901. https://hdl.handle.net/21.15107/rcub_rimsi_2048 .
Janakiev, Tamara, Jelušić, Aleksandra, Kuzmanović, Nemanja, Fira, Đorđe, Dimkić, Ivica, "Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease" in FEMS Conference on Microbiology, Belgrade, Serbia (2022):900-901, https://hdl.handle.net/21.15107/rcub_rimsi_2048 .