TY  - CONF
AU  - Janakiev, Tamara
AU  - Jelušić, Aleksandra
AU  - Kuzmanović, Nemanja
AU  - Fira, Đorđe
AU  - Dimkić, Ivica
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2048
AB  - BACKGROUND
Atypical Rhizobium sp. were identified as a causative agent of crown gall on rhododendron and blueberry in Germany. Considering the lack of efficient measures against crown gall disease, evaluation of innovative biocontrol measures would greatly contribute to sustainable agriculture.
OBJECTIVES
Identification of candidate bacterial strains that could be employed for biological control of the novel crown gall bacteria belonging to the ″tumorigenes″ clade.
METHODS
Antimicrobial activity of six antagonistic Pseudomonas and Bacillus strains were tested in vitro and in vivo against pathogenic strain Rhizobium sp. rho-6.2. The in vivo experiment, using co-inoculation and preventive inoculation strategies, was performed in controlled greenhouse conditions on tomato plants as a model system in four replicas and randomized.
RESULTS
The whole cultures of two antagonistic Pseudomonas strains were the most efficient against pathogenic Rhizobium sp. rho-6.2 in co-inoculation strategy with reducing tumor size of 92.86%. Contrary, in the preventive treatment same Pseudomonas strains were less efficient (15.38 and 30.77%). Despite the high in vitro antimicrobial activity of Bacillus strains their in vivo activity was less pronounced in preventive treatment (up to 15.38%), while in co-inoculation strategy was more prominent (42.86%). Consortium based on Bacillus and Pseudomonas strains, was increased biocontrol activity up to 38.6% of tumor’s reduction. The selected Pseudomonas strains
could be further tested as an alternative strategy for the biocontrol of crown gall disease and potential involvement of quorum quenching mechanism will be determined.
PB  - Federation of European Microbiological Societies
C3  - FEMS Conference on Microbiology, Belgrade, Serbia
T1  - Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease
EP  - 901
SP  - 900
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2048
ER  - 

@conference{
author = "Janakiev, Tamara and Jelušić, Aleksandra and Kuzmanović, Nemanja and Fira, Đorđe and Dimkić, Ivica",
year = "2022",
abstract = "BACKGROUND
Atypical Rhizobium sp. were identified as a causative agent of crown gall on rhododendron and blueberry in Germany. Considering the lack of efficient measures against crown gall disease, evaluation of innovative biocontrol measures would greatly contribute to sustainable agriculture.
OBJECTIVES
Identification of candidate bacterial strains that could be employed for biological control of the novel crown gall bacteria belonging to the ″tumorigenes″ clade.
METHODS
Antimicrobial activity of six antagonistic Pseudomonas and Bacillus strains were tested in vitro and in vivo against pathogenic strain Rhizobium sp. rho-6.2. The in vivo experiment, using co-inoculation and preventive inoculation strategies, was performed in controlled greenhouse conditions on tomato plants as a model system in four replicas and randomized.
RESULTS
The whole cultures of two antagonistic Pseudomonas strains were the most efficient against pathogenic Rhizobium sp. rho-6.2 in co-inoculation strategy with reducing tumor size of 92.86%. Contrary, in the preventive treatment same Pseudomonas strains were less efficient (15.38 and 30.77%). Despite the high in vitro antimicrobial activity of Bacillus strains their in vivo activity was less pronounced in preventive treatment (up to 15.38%), while in co-inoculation strategy was more prominent (42.86%). Consortium based on Bacillus and Pseudomonas strains, was increased biocontrol activity up to 38.6% of tumor’s reduction. The selected Pseudomonas strains
could be further tested as an alternative strategy for the biocontrol of crown gall disease and potential involvement of quorum quenching mechanism will be determined.",
publisher = "Federation of European Microbiological Societies",
journal = "FEMS Conference on Microbiology, Belgrade, Serbia",
title = "Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease",
pages = "901-900",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2048"
}

Janakiev, T., Jelušić, A., Kuzmanović, N., Fira, Đ.,& Dimkić, I.. (2022). Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease. in FEMS Conference on Microbiology, Belgrade, Serbia
Federation of European Microbiological Societies., 900-901.
https://hdl.handle.net/21.15107/rcub_rimsi_2048

Janakiev T, Jelušić A, Kuzmanović N, Fira Đ, Dimkić I. Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease. in FEMS Conference on Microbiology, Belgrade, Serbia. 2022;:900-901.
https://hdl.handle.net/21.15107/rcub_rimsi_2048 .

Janakiev, Tamara, Jelušić, Aleksandra, Kuzmanović, Nemanja, Fira, Đorđe, Dimkić, Ivica, "Pseudomonas spp. vs. tumorigenic Rhizobium sp. – biocontrol of crown gall disease" in FEMS Conference on Microbiology, Belgrade, Serbia (2022):900-901,
https://hdl.handle.net/21.15107/rcub_rimsi_2048 .