TY  - CONF
AU  - Janošević, Dušica
AU  - Vojisavljević, Katarina
AU  - Pecinar, Ilinka
AU  - Mitrović, Aleksandra Lj.
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1812
AB  - The plant cell wall (CW) is a cell compartment lying outside the plasma
membrane forming a continuum throughout the plant body. The composition of
CWs varies between plant species and cell types, while changes in CW constituents
occur as a result of growth and developmental processes, or as a response to various
environmental stimuli. In the herein study, we present a selection of microscopic
methods for rapid, relatively simple, and low-cost visualization of CW
structure/composition in tissues of stems in trees, shrubs, or herbs. Selected
microscopic methods imply the use of unfixed, intact, both fresh and dried, plant
tissues for the analysis, as well as free-hand or microtome sectioning. UV
microscopy, phloroglucinol-HCl and toluidine blue O staining, SEM microscopy,
and RAMAN microspectroscopy, separately or in combination, can provide valuable
information in plant ecology, plant physiology studies, or for applications in
agronomy and forest products industry. As a model, stem samples of juvenile
Populus x euramericana trees exposed to severe long term static bending were
selected: tension wood fibers (gelatinous fibers, specialized sclerenchyma cells)
could be considered as representatives of CWs with the most complex structure,
while the genus Populus is considered as a model woody Angiosperm.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš Institute for Nature Conservation of Serbia
C3  - 14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022
T1  - Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties
SP  - 201
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1812
ER  - 

@conference{
author = "Janošević, Dušica and Vojisavljević, Katarina and Pecinar, Ilinka and Mitrović, Aleksandra Lj.",
year = "2022",
abstract = "The plant cell wall (CW) is a cell compartment lying outside the plasma
membrane forming a continuum throughout the plant body. The composition of
CWs varies between plant species and cell types, while changes in CW constituents
occur as a result of growth and developmental processes, or as a response to various
environmental stimuli. In the herein study, we present a selection of microscopic
methods for rapid, relatively simple, and low-cost visualization of CW
structure/composition in tissues of stems in trees, shrubs, or herbs. Selected
microscopic methods imply the use of unfixed, intact, both fresh and dried, plant
tissues for the analysis, as well as free-hand or microtome sectioning. UV
microscopy, phloroglucinol-HCl and toluidine blue O staining, SEM microscopy,
and RAMAN microspectroscopy, separately or in combination, can provide valuable
information in plant ecology, plant physiology studies, or for applications in
agronomy and forest products industry. As a model, stem samples of juvenile
Populus x euramericana trees exposed to severe long term static bending were
selected: tension wood fibers (gelatinous fibers, specialized sclerenchyma cells)
could be considered as representatives of CWs with the most complex structure,
while the genus Populus is considered as a model woody Angiosperm.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš Institute for Nature Conservation of Serbia",
journal = "14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022",
title = "Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties",
pages = "201",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1812"
}

Janošević, D., Vojisavljević, K., Pecinar, I.,& Mitrović, A. Lj.. (2022). Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties. in 14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš Institute for Nature Conservation of Serbia., 201.
https://hdl.handle.net/21.15107/rcub_rimsi_1812

Janošević D, Vojisavljević K, Pecinar I, Mitrović AL. Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties. in 14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022. 2022;:201.
https://hdl.handle.net/21.15107/rcub_rimsi_1812 .

Janošević, Dušica, Vojisavljević, Katarina, Pecinar, Ilinka, Mitrović, Aleksandra Lj., "Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties" in 14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022 (2022):201,
https://hdl.handle.net/21.15107/rcub_rimsi_1812 .

TY  - CONF
AU  - Mitrović, Aleksandra Lj.
AU  - Simonović Radosavljević, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Steinbach, Gabor
AU  - Janošević, Dušica
AU  - Vuković, Marina
AU  - Garab, Gyozo
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2987
AB  - Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues:
fibers, vessel elements and tracheids. The contribution of parenchyma cells to stem twining
is mostly unknown. Plants control the orientation and alignment of cellulose fibrils during the
deposition in the cell walls with high precision, creating required anisotropy of the cell wall. Our
aim was to determine possible changes in cellulose fibrils orientation and structural order in stem
parenchyma cell walls related to stem twinning in liana plants.
We applied different microscopy techniques: light microscopy, scanning electron microscopy
and differential polarization laser scanning microscopy (DP-LSM) for fluorescence detected linear
dichroism imaging (FDLD), on stem cross sections of straight and twisted internodes of monocotyledonous
liana Dioscorea balcanica. Histochemical analysis showed no difference in parenchyma
cell wall structure between straight and twisted internodes. Also, no difference in ‘‘cellulose fiber
order’’ in parenchyma cell walls related to stem twining was found by FDLD microscopy. However,
SEM micrographs suggested the difference in cellulose microfibril orientation in secondary cell
walls of parenchyma cells related to stem twining.
Our results indicate that adaptations to stem twining in liana plants involve modifications in
cellulose microfibril orientation in parenchyma cell walls. Although the orientation of cellulose
microfibrils dictates, among other properties, cell shape, living stem parenchyma cells in D. balcanica
retain their shape regardless of stem twining, which is possibly enabled by retaining ‘‘cellulose
fibril order’’.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research "Siniša Stankovic", University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2987
ER  - 

@conference{
author = "Mitrović, Aleksandra Lj. and Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Steinbach, Gabor and Janošević, Dušica and Vuković, Marina and Garab, Gyozo and Radotić, Ksenija",
year = "2018",
abstract = "Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues:
fibers, vessel elements and tracheids. The contribution of parenchyma cells to stem twining
is mostly unknown. Plants control the orientation and alignment of cellulose fibrils during the
deposition in the cell walls with high precision, creating required anisotropy of the cell wall. Our
aim was to determine possible changes in cellulose fibrils orientation and structural order in stem
parenchyma cell walls related to stem twinning in liana plants.
We applied different microscopy techniques: light microscopy, scanning electron microscopy
and differential polarization laser scanning microscopy (DP-LSM) for fluorescence detected linear
dichroism imaging (FDLD), on stem cross sections of straight and twisted internodes of monocotyledonous
liana Dioscorea balcanica. Histochemical analysis showed no difference in parenchyma
cell wall structure between straight and twisted internodes. Also, no difference in ‘‘cellulose fiber
order’’ in parenchyma cell walls related to stem twining was found by FDLD microscopy. However,
SEM micrographs suggested the difference in cellulose microfibril orientation in secondary cell
walls of parenchyma cells related to stem twining.
Our results indicate that adaptations to stem twining in liana plants involve modifications in
cellulose microfibril orientation in parenchyma cell walls. Although the orientation of cellulose
microfibrils dictates, among other properties, cell shape, living stem parenchyma cells in D. balcanica
retain their shape regardless of stem twining, which is possibly enabled by retaining ‘‘cellulose
fibril order’’.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research "Siniša Stankovic", University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2987"
}

Mitrović, A. Lj., Simonović Radosavljević, J., Bogdanović Pristov, J., Steinbach, G., Janošević, D., Vuković, M., Garab, G.,& Radotić, K.. (2018). Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Serbian Plant Physiology Society., 19.
https://hdl.handle.net/21.15107/rcub_rimsi_2987

Mitrović AL, Simonović Radosavljević J, Bogdanović Pristov J, Steinbach G, Janošević D, Vuković M, Garab G, Radotić K. Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:19.
https://hdl.handle.net/21.15107/rcub_rimsi_2987 .

Mitrović, Aleksandra Lj., Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Steinbach, Gabor, Janošević, Dušica, Vuković, Marina, Garab, Gyozo, Radotić, Ksenija, "Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):19,
https://hdl.handle.net/21.15107/rcub_rimsi_2987 .

TY  - CONF
AU  - Spasić, Slađana
AU  - Mitrović, Aleksandra Lj.
AU  - Janošević, Dušica
AU  - Budimir, Snežana
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2401
C3  - Book of abstracts: 12 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kopaonik Mt., Kopaonik Serbia
T1  - 2D fractal analysis in plant analytical morphology and microscopy
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2401
ER  - 

@conference{
author = "Spasić, Slađana and Mitrović, Aleksandra Lj. and Janošević, Dušica and Budimir, Snežana",
year = "2016",
journal = "Book of abstracts: 12 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kopaonik Mt., Kopaonik Serbia",
title = "2D fractal analysis in plant analytical morphology and microscopy",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2401"
}

Spasić, S., Mitrović, A. Lj., Janošević, D.,& Budimir, S.. (2016). 2D fractal analysis in plant analytical morphology and microscopy. in Book of abstracts: 12 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kopaonik Mt., Kopaonik Serbia.
https://hdl.handle.net/21.15107/rcub_rimsi_2401

Spasić S, Mitrović AL, Janošević D, Budimir S. 2D fractal analysis in plant analytical morphology and microscopy. in Book of abstracts: 12 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kopaonik Mt., Kopaonik Serbia. 2016;.
https://hdl.handle.net/21.15107/rcub_rimsi_2401 .

Spasić, Slađana, Mitrović, Aleksandra Lj., Janošević, Dušica, Budimir, Snežana, "2D fractal analysis in plant analytical morphology and microscopy" in Book of abstracts: 12 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kopaonik Mt., Kopaonik Serbia (2016),
https://hdl.handle.net/21.15107/rcub_rimsi_2401 .