Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin
Аутори
Mitrović, Aleksandra Lj.Simonović Radosavljević, Jasna
Bogdanović Pristov, Jelena
Steinbach, Gabor
Janošević, Dušica
Vuković, Marina
Garab, Gyozo
Radotić, Ksenija
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues:
fibers, vessel elements and tracheids. The contribution of parenchyma cells to stem twining
is mostly unknown. Plants control the orientation and alignment of cellulose fibrils during the
deposition in the cell walls with high precision, creating required anisotropy of the cell wall. Our
aim was to determine possible changes in cellulose fibrils orientation and structural order in stem
parenchyma cell walls related to stem twinning in liana plants.
We applied different microscopy techniques: light microscopy, scanning electron microscopy
and differential polarization laser scanning microscopy (DP-LSM) for fluorescence detected linear
dichroism imaging (FDLD), on stem cross sections of straight and twisted internodes of monocotyledonous
liana Dioscorea balcanica. Histochemical analysis showed no difference in parenchyma
cell wall structure between straight and twisted internod...es. Also, no difference in ‘‘cellulose fiber
order’’ in parenchyma cell walls related to stem twining was found by FDLD microscopy. However,
SEM micrographs suggested the difference in cellulose microfibril orientation in secondary cell
walls of parenchyma cells related to stem twining.
Our results indicate that adaptations to stem twining in liana plants involve modifications in
cellulose microfibril orientation in parenchyma cell walls. Although the orientation of cellulose
microfibrils dictates, among other properties, cell shape, living stem parenchyma cells in D. balcanica
retain their shape regardless of stem twining, which is possibly enabled by retaining ‘‘cellulose
fibril order’’.
Кључне речи:
cellulose, cell wall, parenchyma, Dioscorea balcanica KošaninИзвор:
3rd International Conference on Plant Biology (22nd SPPS Meeting), 2018, 19-Издавач:
- Serbian Plant Physiology Society
- Institute for Biological Research "Siniša Stankovic", University of Belgrade
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Mitrović, Aleksandra Lj. AU - Simonović Radosavljević, Jasna AU - Bogdanović Pristov, Jelena AU - Steinbach, Gabor AU - Janošević, Dušica AU - Vuković, Marina AU - Garab, Gyozo AU - Radotić, Ksenija PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2987 AB - Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. The contribution of parenchyma cells to stem twining is mostly unknown. Plants control the orientation and alignment of cellulose fibrils during the deposition in the cell walls with high precision, creating required anisotropy of the cell wall. Our aim was to determine possible changes in cellulose fibrils orientation and structural order in stem parenchyma cell walls related to stem twinning in liana plants. We applied different microscopy techniques: light microscopy, scanning electron microscopy and differential polarization laser scanning microscopy (DP-LSM) for fluorescence detected linear dichroism imaging (FDLD), on stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica. Histochemical analysis showed no difference in parenchyma cell wall structure between straight and twisted internodes. Also, no difference in ‘‘cellulose fiber order’’ in parenchyma cell walls related to stem twining was found by FDLD microscopy. However, SEM micrographs suggested the difference in cellulose microfibril orientation in secondary cell walls of parenchyma cells related to stem twining. Our results indicate that adaptations to stem twining in liana plants involve modifications in cellulose microfibril orientation in parenchyma cell walls. Although the orientation of cellulose microfibrils dictates, among other properties, cell shape, living stem parenchyma cells in D. balcanica retain their shape regardless of stem twining, which is possibly enabled by retaining ‘‘cellulose fibril order’’. PB - Serbian Plant Physiology Society PB - Institute for Biological Research "Siniša Stankovic", University of Belgrade C3 - 3rd International Conference on Plant Biology (22nd SPPS Meeting) T1 - Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin SP - 19 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2987 ER -
@conference{ author = "Mitrović, Aleksandra Lj. and Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Steinbach, Gabor and Janošević, Dušica and Vuković, Marina and Garab, Gyozo and Radotić, Ksenija", year = "2018", abstract = "Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. The contribution of parenchyma cells to stem twining is mostly unknown. Plants control the orientation and alignment of cellulose fibrils during the deposition in the cell walls with high precision, creating required anisotropy of the cell wall. Our aim was to determine possible changes in cellulose fibrils orientation and structural order in stem parenchyma cell walls related to stem twinning in liana plants. We applied different microscopy techniques: light microscopy, scanning electron microscopy and differential polarization laser scanning microscopy (DP-LSM) for fluorescence detected linear dichroism imaging (FDLD), on stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica. Histochemical analysis showed no difference in parenchyma cell wall structure between straight and twisted internodes. Also, no difference in ‘‘cellulose fiber order’’ in parenchyma cell walls related to stem twining was found by FDLD microscopy. However, SEM micrographs suggested the difference in cellulose microfibril orientation in secondary cell walls of parenchyma cells related to stem twining. Our results indicate that adaptations to stem twining in liana plants involve modifications in cellulose microfibril orientation in parenchyma cell walls. Although the orientation of cellulose microfibrils dictates, among other properties, cell shape, living stem parenchyma cells in D. balcanica retain their shape regardless of stem twining, which is possibly enabled by retaining ‘‘cellulose fibril order’’.", publisher = "Serbian Plant Physiology Society, Institute for Biological Research "Siniša Stankovic", University of Belgrade", journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)", title = "Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin", pages = "19", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2987" }
Mitrović, A. Lj., Simonović Radosavljević, J., Bogdanović Pristov, J., Steinbach, G., Janošević, D., Vuković, M., Garab, G.,& Radotić, K.. (2018). Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin. in 3rd International Conference on Plant Biology (22nd SPPS Meeting) Serbian Plant Physiology Society., 19. https://hdl.handle.net/21.15107/rcub_rimsi_2987
Mitrović AL, Simonović Radosavljević J, Bogdanović Pristov J, Steinbach G, Janošević D, Vuković M, Garab G, Radotić K. Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:19. https://hdl.handle.net/21.15107/rcub_rimsi_2987 .
Mitrović, Aleksandra Lj., Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Steinbach, Gabor, Janošević, Dušica, Vuković, Marina, Garab, Gyozo, Radotić, Ksenija, "Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):19, https://hdl.handle.net/21.15107/rcub_rimsi_2987 .