TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2707
AB  - The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.
PB  - MDPI
T2  - Plants
T1  - Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle
IS  - 24
VL  - 11
DO  - https://doi.org/10.3390/plants11243587
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Šešlija Jovanović, Darka and Ćalić, Dušica and Milanović, Slobodan and Kostić, Miroslav",
year = "2022",
abstract = "The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.",
publisher = "MDPI",
journal = "Plants",
title = "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle",
number = "24",
volume = "11",
doi = "https://doi.org/10.3390/plants11243587"
}

Lazarević, J., Kostić, I., Šešlija Jovanović, D., Ćalić, D., Milanović, S.,& Kostić, M.. (2022). Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants
MDPI., 11(24).
https://doi.org/https://doi.org/10.3390/plants11243587

Lazarević J, Kostić I, Šešlija Jovanović D, Ćalić D, Milanović S, Kostić M. Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants. 2022;11(24).
doi:https://doi.org/10.3390/plants11243587 .

Lazarević, Jelica, Kostić, Igor, Šešlija Jovanović, Darka, Ćalić, Dušica, Milanović, Slobodan, Kostić, Miroslav, "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle" in Plants, 11, no. 24 (2022),
https://doi.org/https://doi.org/10.3390/plants11243587 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Seslija-Jovanović, Darka
AU  - Kostic, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1404
AB  - The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood forests. In the search for an environmentally safe means of its control, we assessed the impact of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality, molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with commercial insecticide NeemAzal(R)-T/S (neem). The main compounds in the Eos were trans-anethole in anise; carvone, limonene, and alpha-phellandrene in dill; and trans-anethole and fenchone in fennel seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%, anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs were more effective in reducing growth rate, approximate digestibility and efficiency of conversion of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used for gypsy moth control.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control
IS  - 10
VL  - 10
DO  - 10.3390/plants10102194
ER  - 

@article{
author = "Kostić, Igor and Lazarević, Jelica and Seslija-Jovanović, Darka and Kostic, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood forests. In the search for an environmentally safe means of its control, we assessed the impact of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality, molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with commercial insecticide NeemAzal(R)-T/S (neem). The main compounds in the Eos were trans-anethole in anise; carvone, limonene, and alpha-phellandrene in dill; and trans-anethole and fenchone in fennel seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%, anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs were more effective in reducing growth rate, approximate digestibility and efficiency of conversion of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used for gypsy moth control.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control",
number = "10",
volume = "10",
doi = "10.3390/plants10102194"
}

Kostić, I., Lazarević, J., Seslija-Jovanović, D., Kostic, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants-Basel
MDPI, Basel., 10(10).
https://doi.org/10.3390/plants10102194

Kostić I, Lazarević J, Seslija-Jovanović D, Kostic M, Marković T, Milanović S. Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants-Basel. 2021;10(10).
doi:10.3390/plants10102194 .

Kostić, Igor, Lazarević, Jelica, Seslija-Jovanović, Darka, Kostic, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control" in Plants-Basel, 10, no. 10 (2021),
https://doi.org/10.3390/plants10102194 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Seslija-Jovanović, Darka
AU  - Krnjajić, Slobodan
AU  - Calic, Dusica D
AU  - Stanković, Sladan
AU  - Kostic, Miroslav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1506
AB  - The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)
EP  - 199
IS  - 2
SP  - 190
VL  - 111
DO  - 10.1017/S0007485320000504
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Milanović, Slobodan and Seslija-Jovanović, Darka and Krnjajić, Slobodan and Calic, Dusica D and Stanković, Sladan and Kostic, Miroslav",
year = "2021",
abstract = "The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)",
pages = "199-190",
number = "2",
volume = "111",
doi = "10.1017/S0007485320000504"
}

Lazarević, J., Kostić, I., Milanović, S., Seslija-Jovanović, D., Krnjajić, S., Calic, D. D., Stanković, S.,& Kostic, M.. (2021). Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 111(2), 190-199.
https://doi.org/10.1017/S0007485320000504

Lazarević J, Kostić I, Milanović S, Seslija-Jovanović D, Krnjajić S, Calic DD, Stanković S, Kostic M. Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research. 2021;111(2):190-199.
doi:10.1017/S0007485320000504 .

Lazarević, Jelica, Kostić, Igor, Milanović, Slobodan, Seslija-Jovanović, Darka, Krnjajić, Slobodan, Calic, Dusica D, Stanković, Sladan, Kostic, Miroslav, "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)" in Bulletin of Entomological Research, 111, no. 2 (2021):190-199,
https://doi.org/10.1017/S0007485320000504 . .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Popović, Marija M.
AU  - Dobrosavljević, Jovan N.
AU  - Kostić, Igor
AU  - Lazarević, Jelica
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1379
AB  - Gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) feeds on a large number of tree species, while ash, Fraxinus spp. (Lamiales: Oleaceae) species are considered resistant and are only sporadically eaten. To assess the conditions under which late instar gypsy moth larvae (GML) can temporarily use non-host common ash ( CA) (F. excelsior L.), and to evaluate their ability to recover from ingestion of this toxic food, we determined the relative growth rate, the relative consumption rate and the amount of produced feces in different laboratory feeding trials. Our report is the first to show that under specific circumstances, the resources acquired after short-term consumption of CA leaves can be utilized for larval growth. We varied the intensity of density and starvation stress prior to feeding on CA leaves. We observed that after moderate stress a group of GML was temporarily capable of coping with CA leaves. Although observed growth and consumption were much lower on CA than on the optimal host oak, Quercus cerris L. (Fagales: Fagaceae), CA-oak-switched larvae showed the ability to recover from short-term use of a toxic non-host foliage. This suggests that feeding on CA might enable GML to survive under conditions of food shortage.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress
EP  - 69
IS  - 1
SP  - 63
VL  - 72
DO  - 10.2298/ABS191106067M
ER  - 

@article{
author = "Milanović, Slobodan and Popović, Marija M. and Dobrosavljević, Jovan N. and Kostić, Igor and Lazarević, Jelica",
year = "2020",
abstract = "Gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) feeds on a large number of tree species, while ash, Fraxinus spp. (Lamiales: Oleaceae) species are considered resistant and are only sporadically eaten. To assess the conditions under which late instar gypsy moth larvae (GML) can temporarily use non-host common ash ( CA) (F. excelsior L.), and to evaluate their ability to recover from ingestion of this toxic food, we determined the relative growth rate, the relative consumption rate and the amount of produced feces in different laboratory feeding trials. Our report is the first to show that under specific circumstances, the resources acquired after short-term consumption of CA leaves can be utilized for larval growth. We varied the intensity of density and starvation stress prior to feeding on CA leaves. We observed that after moderate stress a group of GML was temporarily capable of coping with CA leaves. Although observed growth and consumption were much lower on CA than on the optimal host oak, Quercus cerris L. (Fagales: Fagaceae), CA-oak-switched larvae showed the ability to recover from short-term use of a toxic non-host foliage. This suggests that feeding on CA might enable GML to survive under conditions of food shortage.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress",
pages = "69-63",
number = "1",
volume = "72",
doi = "10.2298/ABS191106067M"
}

Milanović, S., Popović, M. M., Dobrosavljević, J. N., Kostić, I.,& Lazarević, J.. (2020). Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(1), 63-69.
https://doi.org/10.2298/ABS191106067M

Milanović S, Popović MM, Dobrosavljević JN, Kostić I, Lazarević J. Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress. in Archives of Biological Sciences. 2020;72(1):63-69.
doi:10.2298/ABS191106067M .

Milanović, Slobodan, Popović, Marija M., Dobrosavljević, Jovan N., Kostić, Igor, Lazarević, Jelica, "Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress" in Archives of Biological Sciences, 72, no. 1 (2020):63-69,
https://doi.org/10.2298/ABS191106067M . .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Jankovic-Tomanic, Milena
AU  - Kostić, Igor
AU  - Kostic, Miroslav
AU  - Morina, Filis
AU  - Živanović, Bojana
AU  - Lazarević, Jelica
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/990
AB  - Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.
PB  - Wiley, Hoboken
T2  - Entomologia Experimentalis Et Applicata
T1  - Behavioural and physiological plasticity of gypsy moth larvae to host plant switching
EP  - 162
IS  - 2
SP  - 152
VL  - 158
DO  - 10.1111/eea.12388
ER  - 

@article{
author = "Milanović, Slobodan and Jankovic-Tomanic, Milena and Kostić, Igor and Kostic, Miroslav and Morina, Filis and Živanović, Bojana and Lazarević, Jelica",
year = "2016",
abstract = "Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.",
publisher = "Wiley, Hoboken",
journal = "Entomologia Experimentalis Et Applicata",
title = "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching",
pages = "162-152",
number = "2",
volume = "158",
doi = "10.1111/eea.12388"
}

Milanović, S., Jankovic-Tomanic, M., Kostić, I., Kostic, M., Morina, F., Živanović, B.,& Lazarević, J.. (2016). Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata
Wiley, Hoboken., 158(2), 152-162.
https://doi.org/10.1111/eea.12388

Milanović S, Jankovic-Tomanic M, Kostić I, Kostic M, Morina F, Živanović B, Lazarević J. Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata. 2016;158(2):152-162.
doi:10.1111/eea.12388 .

Milanović, Slobodan, Jankovic-Tomanic, Milena, Kostić, Igor, Kostic, Miroslav, Morina, Filis, Živanović, Bojana, Lazarević, Jelica, "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching" in Entomologia Experimentalis Et Applicata, 158, no. 2 (2016):152-162,
https://doi.org/10.1111/eea.12388 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Petrović, Olivera
AU  - Milanović, Slobodan
AU  - Popović, Zorica
AU  - Stanković, Sladjan
AU  - Todorović, Goran
AU  - Kostić, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2432
AB  - Ethanol solutions of essential oils obtained from Athmanta haynaldii and Myristica fragrans were tested for their toxicity and antifeedant activity against the second instar gypsy moth larvae in the laboratory bioassay. Both isolated oils were subjected to gas chromatography analysis in order to determine their chemical constitutions. Tested oils showed low to moderate larvicidal effect in both residual toxicity test and in chronic larval mortality bioassay. However, antifeedant index achieved by application of tested solutions in feeding choice assay was significantly higher in comparison to control, and almost same as one provided with botanical standard. Low toxic and high antifeedant properties (AF index 85–90%) make these essential oils suitable for integrated pest management programs. Special attention should be paid to further investigation of endemic and rare A. haynalidii in the terms its cultivation and usage of its unique set of biologically active compounds.
T2  - Industrial Crops and Products
T2  - Elsevier
T2  - Elsevier
T1  - Biological activity of essential oils of Athamanta haynaldii and Myristica fragrans to gypsy moth larvae
EP  - 20
SP  - 17
DO  - 10.1016/j.indcrop.2012.03.039
ER  - 

@article{
author = "Kostić, Igor and Petrović, Olivera and Milanović, Slobodan and Popović, Zorica and Stanković, Sladjan and Todorović, Goran and Kostić, Miroslav",
year = "2013",
abstract = "Ethanol solutions of essential oils obtained from Athmanta haynaldii and Myristica fragrans were tested for their toxicity and antifeedant activity against the second instar gypsy moth larvae in the laboratory bioassay. Both isolated oils were subjected to gas chromatography analysis in order to determine their chemical constitutions. Tested oils showed low to moderate larvicidal effect in both residual toxicity test and in chronic larval mortality bioassay. However, antifeedant index achieved by application of tested solutions in feeding choice assay was significantly higher in comparison to control, and almost same as one provided with botanical standard. Low toxic and high antifeedant properties (AF index 85–90%) make these essential oils suitable for integrated pest management programs. Special attention should be paid to further investigation of endemic and rare A. haynalidii in the terms its cultivation and usage of its unique set of biologically active compounds.",
journal = "Industrial Crops and Products, Elsevier, Elsevier",
title = "Biological activity of essential oils of Athamanta haynaldii and Myristica fragrans to gypsy moth larvae",
pages = "20-17",
doi = "10.1016/j.indcrop.2012.03.039"
}

Kostić, I., Petrović, O., Milanović, S., Popović, Z., Stanković, S., Todorović, G.,& Kostić, M.. (2013). Biological activity of essential oils of Athamanta haynaldii and Myristica fragrans to gypsy moth larvae. in Industrial Crops and Products, 17-20.
https://doi.org/10.1016/j.indcrop.2012.03.039

Kostić I, Petrović O, Milanović S, Popović Z, Stanković S, Todorović G, Kostić M. Biological activity of essential oils of Athamanta haynaldii and Myristica fragrans to gypsy moth larvae. in Industrial Crops and Products. 2013;:17-20.
doi:10.1016/j.indcrop.2012.03.039 .

Kostić, Igor, Petrović, Olivera, Milanović, Slobodan, Popović, Zorica, Stanković, Sladjan, Todorović, Goran, Kostić, Miroslav, "Biological activity of essential oils of Athamanta haynaldii and Myristica fragrans to gypsy moth larvae" in Industrial Crops and Products (2013):17-20,
https://doi.org/10.1016/j.indcrop.2012.03.039 . .

TY  - JOUR
AU  - Popović, Z.
AU  - Kostić, M.
AU  - Stanković, S.
AU  - Milanović, Slobodan
AU  - Sivčev, I.
AU  - Kostić, Igor
AU  - Kljajić, P.
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/623
AB  - Ethanol solutions of five fractions obtained from essential oil of sweet basil Ocimum basilicum L. (Lamiales: Lamiaceae) (F1-F5) were tested for their antifeedant properties against 2nd instar gypsy moth larvae, Lymantria dispar L. (Lepidoptera: Lymantriidae), in laboratory non-choice and feeding-choice experiments. Prior to bioassays, the chemical composition of each fraction was determined by gas chromatography analyses. Significant larval deterrence from feeding was achieved by application of tested solutions to fresh leaves of the host plant. The most effective were F1 (0.5%), F4 (0.05, 0.1, and 0.5%), and F5 (0.1 and 0.5%), which provided an antifeedant index > 80% after five days. A low rate of larval mortality was observed in no-choice bioassay. In situ screening of chlorophyll fluorescence as an indicator of plant stress level (assessed by the induced fluorometry) confirmed that the tested compounds did not cause alternations in the photosynthetic efficiency of treated leaves.
PB  - Library of the University of Arizona
T2  - Journal of Insect Science
T1  - Ecologically acceptable usage of derivatives of essential oil of sweet basil, Ocimum basilicum, as antifeedants against larvae of the gypsy moth, Lymantria dispar
VL  - 13
DO  - 10.1673/031.013.16101
ER  - 

@article{
author = "Popović, Z. and Kostić, M. and Stanković, S. and Milanović, Slobodan and Sivčev, I. and Kostić, Igor and Kljajić, P.",
year = "2013",
abstract = "Ethanol solutions of five fractions obtained from essential oil of sweet basil Ocimum basilicum L. (Lamiales: Lamiaceae) (F1-F5) were tested for their antifeedant properties against 2nd instar gypsy moth larvae, Lymantria dispar L. (Lepidoptera: Lymantriidae), in laboratory non-choice and feeding-choice experiments. Prior to bioassays, the chemical composition of each fraction was determined by gas chromatography analyses. Significant larval deterrence from feeding was achieved by application of tested solutions to fresh leaves of the host plant. The most effective were F1 (0.5%), F4 (0.05, 0.1, and 0.5%), and F5 (0.1 and 0.5%), which provided an antifeedant index > 80% after five days. A low rate of larval mortality was observed in no-choice bioassay. In situ screening of chlorophyll fluorescence as an indicator of plant stress level (assessed by the induced fluorometry) confirmed that the tested compounds did not cause alternations in the photosynthetic efficiency of treated leaves.",
publisher = "Library of the University of Arizona",
journal = "Journal of Insect Science",
title = "Ecologically acceptable usage of derivatives of essential oil of sweet basil, Ocimum basilicum, as antifeedants against larvae of the gypsy moth, Lymantria dispar",
volume = "13",
doi = "10.1673/031.013.16101"
}

Popović, Z., Kostić, M., Stanković, S., Milanović, S., Sivčev, I., Kostić, I.,& Kljajić, P.. (2013). Ecologically acceptable usage of derivatives of essential oil of sweet basil, Ocimum basilicum, as antifeedants against larvae of the gypsy moth, Lymantria dispar. in Journal of Insect Science
Library of the University of Arizona., 13.
https://doi.org/10.1673/031.013.16101

Popović Z, Kostić M, Stanković S, Milanović S, Sivčev I, Kostić I, Kljajić P. Ecologically acceptable usage of derivatives of essential oil of sweet basil, Ocimum basilicum, as antifeedants against larvae of the gypsy moth, Lymantria dispar. in Journal of Insect Science. 2013;13.
doi:10.1673/031.013.16101 .

Popović, Z., Kostić, M., Stanković, S., Milanović, Slobodan, Sivčev, I., Kostić, Igor, Kljajić, P., "Ecologically acceptable usage of derivatives of essential oil of sweet basil, Ocimum basilicum, as antifeedants against larvae of the gypsy moth, Lymantria dispar" in Journal of Insect Science, 13 (2013),
https://doi.org/10.1673/031.013.16101 . .