TY  - JOUR
AU  - Avdović, Edina H.
AU  - Petrović, Isidora P.
AU  - Stevanović, Milena J.
AU  - Saso, Luciano
AU  - Dimitrić-Marković, Jasmina
AU  - Filipović, Nenad D.
AU  - Zivić, Miroslav
AU  - Cvetic, Antic, Tijana N.
AU  - Žižić, Milan
AU  - Todorović, Nataša
AU  - Vukic, Milena
AU  - Trifunović, Srecko R.
AU  - Marković, Zoran S.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1457
AB  - Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes
VL  - 2021
DO  - 10.1155/2021/8849568
ER  - 

@article{
author = "Avdović, Edina H. and Petrović, Isidora P. and Stevanović, Milena J. and Saso, Luciano and Dimitrić-Marković, Jasmina and Filipović, Nenad D. and Zivić, Miroslav and Cvetic, Antic, Tijana N. and Žižić, Milan and Todorović, Nataša and Vukic, Milena and Trifunović, Srecko R. and Marković, Zoran S.",
year = "2021",
abstract = "Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes",
volume = "2021",
doi = "10.1155/2021/8849568"
}

Avdović, E. H., Petrović, I. P., Stevanović, M. J., Saso, L., Dimitrić-Marković, J., Filipović, N. D., Zivić, M., Cvetic, A. T. N., Žižić, M., Todorović, N., Vukic, M., Trifunović, S. R.,& Marković, Z. S.. (2021). Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2021.
https://doi.org/10.1155/2021/8849568

Avdović EH, Petrović IP, Stevanović MJ, Saso L, Dimitrić-Marković J, Filipović ND, Zivić M, Cvetic ATN, Žižić M, Todorović N, Vukic M, Trifunović SR, Marković ZS. Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity. 2021;2021.
doi:10.1155/2021/8849568 .

Avdović, Edina H., Petrović, Isidora P., Stevanović, Milena J., Saso, Luciano, Dimitrić-Marković, Jasmina, Filipović, Nenad D., Zivić, Miroslav, Cvetic, Antic, Tijana N., Žižić, Milan, Todorović, Nataša, Vukic, Milena, Trifunović, Srecko R., Marković, Zoran S., "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes" in Oxidative Medicine and Cellular Longevity, 2021 (2021),
https://doi.org/10.1155/2021/8849568 . .

TY  - CONF
AU  - Popović, Nikolina
AU  - Prodanović, Olivera
AU  - Gadjanski, Ivana
AU  - Cvetković, Danijela
AU  - Živanović, Marko
AU  - Pavlović, Vladimir
AU  - Filipović, Nenad D.
AU  - Prodanović, Radivoje
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3123
AB  - Carboxymethylcellulose (CMC) is water-soluble cellulose ether which is used in food and cosmetics industry. It also
has big potential for use in pharmaceutical products due to its high biocompatibility, biodegradibility, low immunogenicity and
low price. Crosslinked CMC can absorb large amounts of water and swell to form hydrogels with great physical properties.
The need for new biomaterials and hydrogels is growing daily, due to their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we modified CMC, in order to get a crosslinkable polymer that can make
hydrogels by chemical and enzymatic means. After periodate oxidation of CMC we obtained CMC with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further modification using reductive amination in the presence of different phenolic
compounds like tyramine, was done. This modification of CMC was confirmed by UV–VIS and FT-IR spectroscopy, while
concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid–base titration. All CMCtyramines
were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. CMC
derivatives have been successfully electrospun and crosslinked afterwards. Due to the introduction of amino groups and
decrease in molecular weight, they were significantly more soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also improved. We aim to make nanofibers using tyramine-polysaccharides that
will be more stable in cell culture media after cross-linking covalently and with calcium/barium ions. Diameter of nanofibers
was determined by scanning electron microscopy (SEM). Cross-linked nanofibers that we obtained will be used for tissue
engineering of blood vessels.
PB  - West University of Timișoara
C3  - The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar
T1  - MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING
IS  - 2
SP  - S4_P10
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3123
ER  - 

@conference{
author = "Popović, Nikolina and Prodanović, Olivera and Gadjanski, Ivana and Cvetković, Danijela and Živanović, Marko and Pavlović, Vladimir and Filipović, Nenad D. and Prodanović, Radivoje",
year = "2017",
abstract = "Carboxymethylcellulose (CMC) is water-soluble cellulose ether which is used in food and cosmetics industry. It also
has big potential for use in pharmaceutical products due to its high biocompatibility, biodegradibility, low immunogenicity and
low price. Crosslinked CMC can absorb large amounts of water and swell to form hydrogels with great physical properties.
The need for new biomaterials and hydrogels is growing daily, due to their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we modified CMC, in order to get a crosslinkable polymer that can make
hydrogels by chemical and enzymatic means. After periodate oxidation of CMC we obtained CMC with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further modification using reductive amination in the presence of different phenolic
compounds like tyramine, was done. This modification of CMC was confirmed by UV–VIS and FT-IR spectroscopy, while
concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid–base titration. All CMCtyramines
were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. CMC
derivatives have been successfully electrospun and crosslinked afterwards. Due to the introduction of amino groups and
decrease in molecular weight, they were significantly more soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also improved. We aim to make nanofibers using tyramine-polysaccharides that
will be more stable in cell culture media after cross-linking covalently and with calcium/barium ions. Diameter of nanofibers
was determined by scanning electron microscopy (SEM). Cross-linked nanofibers that we obtained will be used for tissue
engineering of blood vessels.",
publisher = "West University of Timișoara",
journal = "The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar",
title = "MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING",
number = "2",
pages = "S4_P10",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3123"
}

Popović, N., Prodanović, O., Gadjanski, I., Cvetković, D., Živanović, M., Pavlović, V., Filipović, N. D.,& Prodanović, R.. (2017). MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING. in The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar
West University of Timișoara., 26(2), S4_P10.
https://hdl.handle.net/21.15107/rcub_rimsi_3123

Popović N, Prodanović O, Gadjanski I, Cvetković D, Živanović M, Pavlović V, Filipović ND, Prodanović R. MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING. in The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar. 2017;26(2):S4_P10.
https://hdl.handle.net/21.15107/rcub_rimsi_3123 .

Popović, Nikolina, Prodanović, Olivera, Gadjanski, Ivana, Cvetković, Danijela, Živanović, Marko, Pavlović, Vladimir, Filipović, Nenad D., Prodanović, Radivoje, "MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING" in The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar, 26, no. 2 (2017):S4_P10,
https://hdl.handle.net/21.15107/rcub_rimsi_3123 .

TY  - CONF
AU  - Popović, Nikolina
AU  - Prodanović, Olivera
AU  - Gadjanski, Ivana
AU  - Cvetković, Danijela
AU  - Živanović, Marko
AU  - Pavlović, Vladimir
AU  - Filipović, Nenad D.
AU  - Prodanović, Radivoje
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3110
AB  - Carboxymethylcellulose (CMC) and alginate (ALG) are
water-soluble polysaccharides used in food and
cosmetics industry. They have big potential for use in
pharmaceutical products due to their high
biocompatibility, biodegradibility, low immunogenicity
and low price. When crosslinked they can absorb large
amounts of water and swell to form hydrogels with
great physical properties. The need for new
biomaterials and hydrogels is growing daily, due to
their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we
modified ALG and CMC, in order to get a cross-linkable
polymer that can make hydrogels by chemical and
enzymatic means. After periodate oxidation we
obtained polysaccharides with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further
modification using reductive amination in the presence
of different phenolic compounds like tyramine, was
done. This modification was confirmed by UV–VIS and
FT-IR spectroscopy, while concentration of phenol and
ionizable groups was determined using absorbance at
275 nm and acid–base titration. All CMC and AlG
tyramines were able to form hydrogels after crosslinking
with horse radish peroxidase (HRP) and
hydrogen peroxide. Both derivatives have been
successfully electrospun and crosslinked afterwards.
Due to the introduction of amino groups and decrease
in molecular weight, they were significantly more
soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also
improved. We aim to make nanofibers using tyraminepolysaccharides
that will be more stable in cell culture
media after cross-linking covalently and with
calcium/barium ions. Diameter of nanofibers was
determined by scanning electron microscopy (SEM).
Cross-linked nanofibers that we obtained will be used
for tissue engineering of blood vessels.
PB  - Serbian Society for Computational Mechanics
C3  - 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia
T1  - Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig
SP  - T.1.4
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3110
ER  - 

@conference{
author = "Popović, Nikolina and Prodanović, Olivera and Gadjanski, Ivana and Cvetković, Danijela and Živanović, Marko and Pavlović, Vladimir and Filipović, Nenad D. and Prodanović, Radivoje",
year = "2017",
abstract = "Carboxymethylcellulose (CMC) and alginate (ALG) are
water-soluble polysaccharides used in food and
cosmetics industry. They have big potential for use in
pharmaceutical products due to their high
biocompatibility, biodegradibility, low immunogenicity
and low price. When crosslinked they can absorb large
amounts of water and swell to form hydrogels with
great physical properties. The need for new
biomaterials and hydrogels is growing daily, due to
their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we
modified ALG and CMC, in order to get a cross-linkable
polymer that can make hydrogels by chemical and
enzymatic means. After periodate oxidation we
obtained polysaccharides with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further
modification using reductive amination in the presence
of different phenolic compounds like tyramine, was
done. This modification was confirmed by UV–VIS and
FT-IR spectroscopy, while concentration of phenol and
ionizable groups was determined using absorbance at
275 nm and acid–base titration. All CMC and AlG
tyramines were able to form hydrogels after crosslinking
with horse radish peroxidase (HRP) and
hydrogen peroxide. Both derivatives have been
successfully electrospun and crosslinked afterwards.
Due to the introduction of amino groups and decrease
in molecular weight, they were significantly more
soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also
improved. We aim to make nanofibers using tyraminepolysaccharides
that will be more stable in cell culture
media after cross-linking covalently and with
calcium/barium ions. Diameter of nanofibers was
determined by scanning electron microscopy (SEM).
Cross-linked nanofibers that we obtained will be used
for tissue engineering of blood vessels.",
publisher = "Serbian Society for Computational Mechanics",
journal = "4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia",
title = "Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig",
pages = "T.1.4",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3110"
}

Popović, N., Prodanović, O., Gadjanski, I., Cvetković, D., Živanović, M., Pavlović, V., Filipović, N. D.,& Prodanović, R.. (2017). Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig. in 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia
Serbian Society for Computational Mechanics., T.1.4.
https://hdl.handle.net/21.15107/rcub_rimsi_3110

Popović N, Prodanović O, Gadjanski I, Cvetković D, Živanović M, Pavlović V, Filipović ND, Prodanović R. Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig. in 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia. 2017;:T.1.4.
https://hdl.handle.net/21.15107/rcub_rimsi_3110 .

Popović, Nikolina, Prodanović, Olivera, Gadjanski, Ivana, Cvetković, Danijela, Živanović, Marko, Pavlović, Vladimir, Filipović, Nenad D., Prodanović, Radivoje, "Modification of Polysaccharides with Phenols for Hydrogels Formation and Electrospinnig" in 4th South-East European Conference on Computational Mechanics, Kragujevac, Serbia (2017):T.1.4,
https://hdl.handle.net/21.15107/rcub_rimsi_3110 .