TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Vuleta, Ana
AU  - Manitasević-Jovanović, Sanja
AU  - Kostic, Miroslav
AU  - Seslija-Jovanović, Darka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1533
AB  - Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Physiology
T1  - Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say
VL  - 13
DO  - 10.3389/fphys.2022.842314
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Vuleta, Ana and Manitasević-Jovanović, Sanja and Kostic, Miroslav and Seslija-Jovanović, Darka",
year = "2022",
abstract = "Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Physiology",
title = "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say",
volume = "13",
doi = "10.3389/fphys.2022.842314"
}

Lazarević, J., Jevremović, S., Kostić, I., Vuleta, A., Manitasević-Jovanović, S., Kostic, M.,& Seslija-Jovanović, D.. (2022). Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology
Frontiers Media Sa, Lausanne., 13.
https://doi.org/10.3389/fphys.2022.842314

Lazarević J, Jevremović S, Kostić I, Vuleta A, Manitasević-Jovanović S, Kostic M, Seslija-Jovanović D. Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology. 2022;13.
doi:10.3389/fphys.2022.842314 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Vuleta, Ana, Manitasević-Jovanović, Sanja, Kostic, Miroslav, Seslija-Jovanović, Darka, "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say" in Frontiers in Physiology, 13 (2022),
https://doi.org/10.3389/fphys.2022.842314 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Seslija-Jovanović, Darka
AU  - Kostic, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1404
AB  - The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood forests. In the search for an environmentally safe means of its control, we assessed the impact of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality, molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with commercial insecticide NeemAzal(R)-T/S (neem). The main compounds in the Eos were trans-anethole in anise; carvone, limonene, and alpha-phellandrene in dill; and trans-anethole and fenchone in fennel seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%, anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs were more effective in reducing growth rate, approximate digestibility and efficiency of conversion of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used for gypsy moth control.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control
IS  - 10
VL  - 10
DO  - 10.3390/plants10102194
ER  - 

@article{
author = "Kostić, Igor and Lazarević, Jelica and Seslija-Jovanović, Darka and Kostic, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood forests. In the search for an environmentally safe means of its control, we assessed the impact of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality, molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with commercial insecticide NeemAzal(R)-T/S (neem). The main compounds in the Eos were trans-anethole in anise; carvone, limonene, and alpha-phellandrene in dill; and trans-anethole and fenchone in fennel seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%, anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs were more effective in reducing growth rate, approximate digestibility and efficiency of conversion of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used for gypsy moth control.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control",
number = "10",
volume = "10",
doi = "10.3390/plants10102194"
}

Kostić, I., Lazarević, J., Seslija-Jovanović, D., Kostic, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants-Basel
MDPI, Basel., 10(10).
https://doi.org/10.3390/plants10102194

Kostić I, Lazarević J, Seslija-Jovanović D, Kostic M, Marković T, Milanović S. Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants-Basel. 2021;10(10).
doi:10.3390/plants10102194 .

Kostić, Igor, Lazarević, Jelica, Seslija-Jovanović, Darka, Kostic, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control" in Plants-Basel, 10, no. 10 (2021),
https://doi.org/10.3390/plants10102194 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Seslija-Jovanović, Darka
AU  - Krnjajić, Slobodan
AU  - Calic, Dusica D
AU  - Stanković, Sladan
AU  - Kostic, Miroslav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1506
AB  - The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)
EP  - 199
IS  - 2
SP  - 190
VL  - 111
DO  - 10.1017/S0007485320000504
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Milanović, Slobodan and Seslija-Jovanović, Darka and Krnjajić, Slobodan and Calic, Dusica D and Stanković, Sladan and Kostic, Miroslav",
year = "2021",
abstract = "The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)",
pages = "199-190",
number = "2",
volume = "111",
doi = "10.1017/S0007485320000504"
}

Lazarević, J., Kostić, I., Milanović, S., Seslija-Jovanović, D., Krnjajić, S., Calic, D. D., Stanković, S.,& Kostic, M.. (2021). Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 111(2), 190-199.
https://doi.org/10.1017/S0007485320000504

Lazarević J, Kostić I, Milanović S, Seslija-Jovanović D, Krnjajić S, Calic DD, Stanković S, Kostic M. Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research. 2021;111(2):190-199.
doi:10.1017/S0007485320000504 .

Lazarević, Jelica, Kostić, Igor, Milanović, Slobodan, Seslija-Jovanović, Darka, Krnjajić, Slobodan, Calic, Dusica D, Stanković, Sladan, Kostic, Miroslav, "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)" in Bulletin of Entomological Research, 111, no. 2 (2021):190-199,
https://doi.org/10.1017/S0007485320000504 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Kostic, Miroslav
AU  - Vuleta, Ana
AU  - Manitasević-Jovanović, Sanja
AU  - Seslija-Jovanović, Darka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1320
AB  - Simple Summary Compounds of botanical origin, including essential oils (EOs), which coevolved as plant defense mechanism against herbivores and pathogens have been proposed as a promising strategy for post-harvest control of stored product insects. Despite several drawbacks, such as low stability, phytotoxicity and food odor changes at high concentrations, EOs are believed to be safe for human and environmental health and thus have an advantage comparing to conventional insecticides. The present study was aimed to examine acute toxicity and effects of thyme (Thymus vulgaris) EO on longevity, behavior and physiology of the bean weevil (Acanthoscelides obtectus), a major pest of stored bean seeds. We found that exposure to thyme oil reduced adult survival and longevity and induced damages to lipids and proteins in a concentration-dependent manner. Sublethal EO concentrations also deterred female egg laying and strongly inhibited adult emergence. Efficacy of such low EO concentrations in suppression of adult emergence implies that thyme EO might be cost-effective and environmentally low risk botanical insecticide for bean seed protection against A. obtectus. The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.
PB  - MDPI, Basel
T2  - Insects
T1  - Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus
IS  - 9
VL  - 11
DO  - 10.3390/insects11090563
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Kostic, Miroslav and Vuleta, Ana and Manitasević-Jovanović, Sanja and Seslija-Jovanović, Darka",
year = "2020",
abstract = "Simple Summary Compounds of botanical origin, including essential oils (EOs), which coevolved as plant defense mechanism against herbivores and pathogens have been proposed as a promising strategy for post-harvest control of stored product insects. Despite several drawbacks, such as low stability, phytotoxicity and food odor changes at high concentrations, EOs are believed to be safe for human and environmental health and thus have an advantage comparing to conventional insecticides. The present study was aimed to examine acute toxicity and effects of thyme (Thymus vulgaris) EO on longevity, behavior and physiology of the bean weevil (Acanthoscelides obtectus), a major pest of stored bean seeds. We found that exposure to thyme oil reduced adult survival and longevity and induced damages to lipids and proteins in a concentration-dependent manner. Sublethal EO concentrations also deterred female egg laying and strongly inhibited adult emergence. Efficacy of such low EO concentrations in suppression of adult emergence implies that thyme EO might be cost-effective and environmentally low risk botanical insecticide for bean seed protection against A. obtectus. The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.",
publisher = "MDPI, Basel",
journal = "Insects",
title = "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus",
number = "9",
volume = "11",
doi = "10.3390/insects11090563"
}

Lazarević, J., Jevremović, S., Kostić, I., Kostic, M., Vuleta, A., Manitasević-Jovanović, S.,& Seslija-Jovanović, D.. (2020). Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects
MDPI, Basel., 11(9).
https://doi.org/10.3390/insects11090563

Lazarević J, Jevremović S, Kostić I, Kostic M, Vuleta A, Manitasević-Jovanović S, Seslija-Jovanović D. Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects. 2020;11(9).
doi:10.3390/insects11090563 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Kostic, Miroslav, Vuleta, Ana, Manitasević-Jovanović, Sanja, Seslija-Jovanović, Darka, "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus" in Insects, 11, no. 9 (2020),
https://doi.org/10.3390/insects11090563 . .

TY  - JOUR
AU  - Jevremović, Stojan
AU  - Lazarević, Jelica
AU  - Kostic, Miroslav
AU  - Krnjajić, Slobodan
AU  - Ugrenović, Vladan
AU  - Radonjic, Anda
AU  - Kostić, Igor
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1252
AB  - The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, alpha-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and a-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans
EP  - 676
IS  - 4
SP  - 665
VL  - 71
DO  - 10.2298/ABS190617049J
ER  - 

@article{
author = "Jevremović, Stojan and Lazarević, Jelica and Kostic, Miroslav and Krnjajić, Slobodan and Ugrenović, Vladan and Radonjic, Anda and Kostić, Igor",
year = "2019",
abstract = "The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, alpha-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and a-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans",
pages = "676-665",
number = "4",
volume = "71",
doi = "10.2298/ABS190617049J"
}

Jevremović, S., Lazarević, J., Kostic, M., Krnjajić, S., Ugrenović, V., Radonjic, A.,& Kostić, I.. (2019). Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(4), 665-676.
https://doi.org/10.2298/ABS190617049J

Jevremović S, Lazarević J, Kostic M, Krnjajić S, Ugrenović V, Radonjic A, Kostić I. Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences. 2019;71(4):665-676.
doi:10.2298/ABS190617049J .

Jevremović, Stojan, Lazarević, Jelica, Kostic, Miroslav, Krnjajić, Slobodan, Ugrenović, Vladan, Radonjic, Anda, Kostić, Igor, "Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans" in Archives of Biological Sciences, 71, no. 4 (2019):665-676,
https://doi.org/10.2298/ABS190617049J . .

TY  - JOUR
AU  - Zabel, Anton
AU  - Stanković, Slađan
AU  - Kostic, Miroslav
AU  - Rahović, Dragan
AU  - Tomic, Vedran
AU  - Kostić, Igor
AU  - Alkhammas, Iman Omar
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1025
AB  - The resistance levels of the Colorado potato beetle (CPB) in Serbia to organophosphorus (OP) and carbamate (C) insecticides varied by population. The resistance level of populations to OPs indescending order was: Futog, Ratari, Dobanovci and Pester. Most resistant to carbarylwere populations Dobanovci and Ratari, followed by Futog, Kaona, and Pester, respectively. The order of resistance levels for OPs and C was completely opposite. Experiments showed that acetylcholinesterase (AChE) activity of CPB was verypronounced and easily measured. At a constant AChE concentration, increasing thesubstrate concentration will cause a positive, linear and dependent increase in thereaction. The same applies in the reaction with constant substrate concentration andincreased enzyme concentrations. AChE activity is significantly affected not only bylocation but also by substrate concentration (acetylthiocholine iodide ATChI). At low substrate concentrations, the highest AChE activity was determined in population Dobanovci, followed by Kaona, Ratari and Futog, respectively. At high substrate concentrations, however, the highest AChE activity was determined in population Ratari and then in Dobanovci and Futog. The lowest rate was in population Kaona. Considering that ATChI (substrate) in increased concentrations inhibits normal AChE activity, it can be concluded that altered AChE affected the change in the populationorder. The testing of AChE in the presence of high concentrations of substrate showsthe portion of altered AChE is largest in population Ratari, and yet considerably smallerin Dobanovci, Futog and Kaona. The total AChE activity is in correlation with thedetermined resistance to carbamates.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates
EP  - 12596
IS  - 3
SP  - 12584
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1025
ER  - 

@article{
author = "Zabel, Anton and Stanković, Slađan and Kostic, Miroslav and Rahović, Dragan and Tomic, Vedran and Kostić, Igor and Alkhammas, Iman Omar",
year = "2017",
abstract = "The resistance levels of the Colorado potato beetle (CPB) in Serbia to organophosphorus (OP) and carbamate (C) insecticides varied by population. The resistance level of populations to OPs indescending order was: Futog, Ratari, Dobanovci and Pester. Most resistant to carbarylwere populations Dobanovci and Ratari, followed by Futog, Kaona, and Pester, respectively. The order of resistance levels for OPs and C was completely opposite. Experiments showed that acetylcholinesterase (AChE) activity of CPB was verypronounced and easily measured. At a constant AChE concentration, increasing thesubstrate concentration will cause a positive, linear and dependent increase in thereaction. The same applies in the reaction with constant substrate concentration andincreased enzyme concentrations. AChE activity is significantly affected not only bylocation but also by substrate concentration (acetylthiocholine iodide ATChI). At low substrate concentrations, the highest AChE activity was determined in population Dobanovci, followed by Kaona, Ratari and Futog, respectively. At high substrate concentrations, however, the highest AChE activity was determined in population Ratari and then in Dobanovci and Futog. The lowest rate was in population Kaona. Considering that ATChI (substrate) in increased concentrations inhibits normal AChE activity, it can be concluded that altered AChE affected the change in the populationorder. The testing of AChE in the presence of high concentrations of substrate showsthe portion of altered AChE is largest in population Ratari, and yet considerably smallerin Dobanovci, Futog and Kaona. The total AChE activity is in correlation with thedetermined resistance to carbamates.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates",
pages = "12596-12584",
number = "3",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1025"
}

Zabel, A., Stanković, S., Kostic, M., Rahović, D., Tomic, V., Kostić, I.,& Alkhammas, I. O.. (2017). Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates. in Romanian Biotechnological Letters
Ars Docendi, Bucharest., 22(3), 12584-12596.
https://hdl.handle.net/21.15107/rcub_rimsi_1025

Zabel A, Stanković S, Kostic M, Rahović D, Tomic V, Kostić I, Alkhammas IO. Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates. in Romanian Biotechnological Letters. 2017;22(3):12584-12596.
https://hdl.handle.net/21.15107/rcub_rimsi_1025 .

Zabel, Anton, Stanković, Slađan, Kostic, Miroslav, Rahović, Dragan, Tomic, Vedran, Kostić, Igor, Alkhammas, Iman Omar, "Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates" in Romanian Biotechnological Letters, 22, no. 3 (2017):12584-12596,
https://hdl.handle.net/21.15107/rcub_rimsi_1025 .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Jankovic-Tomanic, Milena
AU  - Kostić, Igor
AU  - Kostic, Miroslav
AU  - Morina, Filis
AU  - Živanović, Bojana
AU  - Lazarević, Jelica
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/990
AB  - Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.
PB  - Wiley, Hoboken
T2  - Entomologia Experimentalis Et Applicata
T1  - Behavioural and physiological plasticity of gypsy moth larvae to host plant switching
EP  - 162
IS  - 2
SP  - 152
VL  - 158
DO  - 10.1111/eea.12388
ER  - 

@article{
author = "Milanović, Slobodan and Jankovic-Tomanic, Milena and Kostić, Igor and Kostic, Miroslav and Morina, Filis and Živanović, Bojana and Lazarević, Jelica",
year = "2016",
abstract = "Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.",
publisher = "Wiley, Hoboken",
journal = "Entomologia Experimentalis Et Applicata",
title = "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching",
pages = "162-152",
number = "2",
volume = "158",
doi = "10.1111/eea.12388"
}

Milanović, S., Jankovic-Tomanic, M., Kostić, I., Kostic, M., Morina, F., Živanović, B.,& Lazarević, J.. (2016). Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata
Wiley, Hoboken., 158(2), 152-162.
https://doi.org/10.1111/eea.12388

Milanović S, Jankovic-Tomanic M, Kostić I, Kostic M, Morina F, Živanović B, Lazarević J. Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata. 2016;158(2):152-162.
doi:10.1111/eea.12388 .

Milanović, Slobodan, Jankovic-Tomanic, Milena, Kostić, Igor, Kostic, Miroslav, Morina, Filis, Živanović, Bojana, Lazarević, Jelica, "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching" in Entomologia Experimentalis Et Applicata, 158, no. 2 (2016):152-162,
https://doi.org/10.1111/eea.12388 . .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Devrnja, Nina M
AU  - Kostić, Igor
AU  - Kostic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/691
AB  - The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca
EP  - 122
SP  - 118
VL  - 155
DO  - 10.1016/j.scienta.2013.03.017
ER  - 

@article{
author = "Calic, Dusica D and Devrnja, Nina M and Kostić, Igor and Kostic, Miroslav",
year = "2013",
abstract = "The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca",
pages = "122-118",
volume = "155",
doi = "10.1016/j.scienta.2013.03.017"
}

Calic, D. D., Devrnja, N. M., Kostić, I.,& Kostic, M.. (2013). Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae
Elsevier, Amsterdam., 155, 118-122.
https://doi.org/10.1016/j.scienta.2013.03.017

Calic DD, Devrnja NM, Kostić I, Kostic M. Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae. 2013;155:118-122.
doi:10.1016/j.scienta.2013.03.017 .

Calic, Dusica D, Devrnja, Nina M, Kostić, Igor, Kostic, Miroslav, "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca" in Scientia Horticulturae, 155 (2013):118-122,
https://doi.org/10.1016/j.scienta.2013.03.017 . .