TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Bataveljic, Danijela
AU  - Bijelic, Dunja
AU  - Milicevic, Katarina
AU  - Bogdanović Pristov, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2015
AB  - The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, 
attention, cognitive function and stress responses. Unlike fast neurotransmitters which 
act at synapses, NE is released in the neuropil and performs multiple targeting in the 
surrounding area. The glial cells astrocytes are a direct target of NE, as they express 
all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial 
responses elicited by strong and transient increases of NE in the brain are well studied, 
but the effect of a low background NE concentration on astrocytes is unknown. This 
background level of NE is maintained by basal noradrenergic activity and is 
constantly present in the brain. Therefore, the response of astrocytes to the 
background NE could have been unintentionally evoked in previous studies but its 
effect overlooked. 
To assess action of background NE on astrocytes we combined the whole-cell patch
clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured 
cortical astrocytes to bypass NE targeting of multiple cell types. 
We show that cortical astrocytes detect and respond to the background NE 
concentration with an increase in intracellular Ca2+. This Ca2+ liberated from 
intracellular stores further increased large-conductance, Ca2+-sensitive potassium 
(BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK 
channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat 
cortex.   Furthermore, stimulation of astrocytes by background NE was inhibited by 
alpha-adrenoceptor antagonist. 
Our results suggest that astrocytes maintain basal brain activity by perceiving and 
responding to the background NE.
PB  - Serbian Neuroscience Society, Belgrade
C3  - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
T1  - Background norepinephrine impacts activity of cortical astrocytes
SP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2015
ER  - 

@conference{
author = "Nikolic, Ljiljana and Bataveljic, Danijela and Bijelic, Dunja and Milicevic, Katarina and Bogdanović Pristov, Jelena",
year = "2023",
abstract = "The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, 
attention, cognitive function and stress responses. Unlike fast neurotransmitters which 
act at synapses, NE is released in the neuropil and performs multiple targeting in the 
surrounding area. The glial cells astrocytes are a direct target of NE, as they express 
all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial 
responses elicited by strong and transient increases of NE in the brain are well studied, 
but the effect of a low background NE concentration on astrocytes is unknown. This 
background level of NE is maintained by basal noradrenergic activity and is 
constantly present in the brain. Therefore, the response of astrocytes to the 
background NE could have been unintentionally evoked in previous studies but its 
effect overlooked. 
To assess action of background NE on astrocytes we combined the whole-cell patch
clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured 
cortical astrocytes to bypass NE targeting of multiple cell types. 
We show that cortical astrocytes detect and respond to the background NE 
concentration with an increase in intracellular Ca2+. This Ca2+ liberated from 
intracellular stores further increased large-conductance, Ca2+-sensitive potassium 
(BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK 
channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat 
cortex.   Furthermore, stimulation of astrocytes by background NE was inhibited by 
alpha-adrenoceptor antagonist. 
Our results suggest that astrocytes maintain basal brain activity by perceiving and 
responding to the background NE.",
publisher = "Serbian Neuroscience Society, Belgrade",
journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS",
title = "Background norepinephrine impacts activity of cortical astrocytes",
pages = "51",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2015"
}

Nikolic, L., Bataveljic, D., Bijelic, D., Milicevic, K.,& Bogdanović Pristov, J.. (2023). Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
Serbian Neuroscience Society, Belgrade., 51.
https://hdl.handle.net/21.15107/rcub_rimsi_2015

Nikolic L, Bataveljic D, Bijelic D, Milicevic K, Bogdanović Pristov J. Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:51.
https://hdl.handle.net/21.15107/rcub_rimsi_2015 .

Nikolic, Ljiljana, Bataveljic, Danijela, Bijelic, Dunja, Milicevic, Katarina, Bogdanović Pristov, Jelena, "Background norepinephrine impacts activity of cortical astrocytes" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):51,
https://hdl.handle.net/21.15107/rcub_rimsi_2015 .