@conference{
author = "Šijačić-Nikolić, Mirjana and Kerkez Janković, Ivona and Nonić, Marina and Maksimović, Filip and Aleksic, Jelena M",
year = "2023",
abstract = "Pedunculate oak (Quercus robur L.) is one of the economically and ecologically most
important deciduous forest tree species in Europe. In recent decades, there has been an
evident decline of pedunculate oak forests, caused by various factors, such as climate change,
site conditions, over-exploitation, or insufficient and inadequate regeneration. In Serbia, this
species usually occurs in the valleys of major rivers (Danube, Sava, and Morava), and the most
valuable pedunculate oak forests grow along the river Sava in the area of Srem, where there
are individual specimens aged hundreds of years. The protected area „Košutnjak Forest“
(Belgrade, Serbia), occupies 267 ha, with numerous forest tree species, among which are five
native oak species, including the pedunculate oak. Oaks can be categorized as „at-risk” species
in „Kosutnjak Forest”, since their populations are mostly represented by very old trees, with a
poor possibility of natural rejuvenation. This research aimed to determine the genetic
variability of the pedunculate oak population in the „Košutnjak Forest” using nuclear
microsatellites. DNA extraction was performed from young leaves, collected from 56 adult
trees, using a commercial peqGOLD Plant DNA Mini Kit (PEQLAB). In total 13 nuclear
microsatellites were used, and values of standard genetic diversity parameters were
calculated using GenAlEx 6.5 software. The number of alleles per locus ranged from 8 (MSQ13
and PIE239) to 39 (QrZAG90), with an average of 17.538. The number of effective alleles per
locus was in the range from 2,498 (QrZAG108) to 23.668 (QrZAG90), with an average of 8.360.
The average value of the observed heterozygosity (Ho) was 0.705, and the average unbiased
expected heterozygosity (uHe) was 0.818. A statistically significant deviation of the expected
heterozygosity from the observed heterozygosity occurs at 5 loci (MAQ4, PIE239, QpZAG104,
QrZAG108, and MSQ13). The average values of the fixation index were positive and
statistically significant, indicating an excess of homozygotes. According to the assessment of
the genetic status, determining the level of genetic variability of pedunculate oak served as a
basis for defining in situ conservation measures for the available gene pool in „Kosutnjak
Forest”.",
publisher = "Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Genetics&Applications, CONUB&H 2023",
title = "GENETIC DIVERSITY OF THE QUERCUS ROBUR L. POPULATION FROM THE PROTECTED AREA „KOŠUTNJAK FOREST” (BELGRADE, SERBIA) ASSESSED BY NUCLEAR MICROSATELLITES",
pages = "83-83",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2773"
}