TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1951
AB  - BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)
SP  - 915
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1951
ER  - 

@conference{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)",
pages = "915",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1951"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Stanković, S.,& Popović, T.. (2022). PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951

Jelušić A, Mitrović P, Marković S, Iličić R, Stanković S, Popović T. PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Stanković, Slaviša, Popović, Tatjana, "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):915,
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Peeters, Kelly
AU  - Miklavcic-Visnjevec, Ana
AU  - Tavzes, Crtomir
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1426
AB  - Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates
VL  - 160
DO  - 10.1016/j.biocontrol.2021.104695
ER  - 

@article{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Peeters, Kelly and Miklavcic-Visnjevec, Ana and Tavzes, Crtomir and Stanković, Slaviša and Berić, Tanja",
year = "2021",
abstract = "Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates",
volume = "160",
doi = "10.1016/j.biocontrol.2021.104695"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Peeters, K., Miklavcic-Visnjevec, A., Tavzes, C., Stanković, S.,& Berić, T.. (2021). Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 160.
https://doi.org/10.1016/j.biocontrol.2021.104695

Jelušić A, Popović T, Dimkić I, Mitrović P, Peeters K, Miklavcic-Visnjevec A, Tavzes C, Stanković S, Berić T. Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control. 2021;160.
doi:10.1016/j.biocontrol.2021.104695 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Peeters, Kelly, Miklavcic-Visnjevec, Ana, Tavzes, Crtomir, Stanković, Slaviša, Berić, Tanja, "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates" in Biological Control, 160 (2021),
https://doi.org/10.1016/j.biocontrol.2021.104695 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marjanović-Jeromela, Ana
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1407
AB  - Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia
EP  - 49
IS  - 1
SP  - 35
VL  - 70
DO  - 10.1111/ppa.13273
ER  - 

@article{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marjanović-Jeromela, Ana and Popović, Tatjana",
year = "2021",
abstract = "Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia",
pages = "49-35",
number = "1",
volume = "70",
doi = "10.1111/ppa.13273"
}

Jelušić, A., Berić, T., Mitrović, P., Dimkić, I., Stanković, S., Marjanović-Jeromela, A.,& Popović, T.. (2021). New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia. in Plant Pathology
Wiley, Hoboken., 70(1), 35-49.
https://doi.org/10.1111/ppa.13273

Jelušić A, Berić T, Mitrović P, Dimkić I, Stanković S, Marjanović-Jeromela A, Popović T. New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia. in Plant Pathology. 2021;70(1):35-49.
doi:10.1111/ppa.13273 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marjanović-Jeromela, Ana, Popović, Tatjana, "New insights into the genetic diversity ofXanthomonas campestrispv.campestrisisolates from winter oilseed rape in Serbia" in Plant Pathology, 70, no. 1 (2021):35-49,
https://doi.org/10.1111/ppa.13273 . .

TY  - JOUR
AU  - Mitrović, Petar
AU  - Djalović, Ivica
AU  - Kiprovski, Biljana
AU  - Veljović-Jovanović, Sonja
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1459
AB  - The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani
IS  - 2
VL  - 10
DO  - 10.3390/plants10020337
ER  - 

@article{
author = "Mitrović, Petar and Djalović, Ivica and Kiprovski, Biljana and Veljović-Jovanović, Sonja and Trkulja, Vojislav and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani",
number = "2",
volume = "10",
doi = "10.3390/plants10020337"
}

Mitrović, P., Djalović, I., Kiprovski, B., Veljović-Jovanović, S., Trkulja, V., Jelušić, A.,& Popović, T.. (2021). Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel
MDPI, Basel., 10(2).
https://doi.org/10.3390/plants10020337

Mitrović P, Djalović I, Kiprovski B, Veljović-Jovanović S, Trkulja V, Jelušić A, Popović T. Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel. 2021;10(2).
doi:10.3390/plants10020337 .

Mitrović, Petar, Djalović, Ivica, Kiprovski, Biljana, Veljović-Jovanović, Sonja, Trkulja, Vojislav, Jelušić, Aleksandra, Popović, Tatjana, "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani" in Plants-Basel, 10, no. 2 (2021),
https://doi.org/10.3390/plants10020337 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Marković, Sanja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1916
AB  - Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of 
cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which aff ect 
proper cabbage growth, leading to head weight and quality losses and thereby drastically 
reducing its marketing value. The pathogen is genetically diverse, which is evident from the 
presence of eleven races worldwide and more than thirty combinations of allelic profi les. 
Therefore, this study aimed to determine the allelic profi les of Serbian cabbage Xcc strains 
obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was fi rst 
amplifi ed using polymerase chain reaction (PCR) with four housekeeping genes – P-X dnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were fi nally used 
to determine allelic profi les. Allelic profi les were determined by comparison with 33 Xcc 
strains obtained from diff erent hosts and regions, whose allelic profi les had been determined 
previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profi le 
determination and Phyloviz software for constructing a minimum spanning tree. The obtained 
allelic profi le of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and 
rpoD genes, respectively. This profi le is assigned as sequence type 2 (ST2) and it coincides 
with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No 
connection between sequence type (ST) and the host was detected.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage
EP  - 26
IS  - 1
SP  - 19
VL  - 35
DO  - 10.2298/PIF2001019P
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Marković, Sanja",
year = "2020",
abstract = "Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of 
cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which aff ect 
proper cabbage growth, leading to head weight and quality losses and thereby drastically 
reducing its marketing value. The pathogen is genetically diverse, which is evident from the 
presence of eleven races worldwide and more than thirty combinations of allelic profi les. 
Therefore, this study aimed to determine the allelic profi les of Serbian cabbage Xcc strains 
obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was fi rst 
amplifi ed using polymerase chain reaction (PCR) with four housekeeping genes – P-X dnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were fi nally used 
to determine allelic profi les. Allelic profi les were determined by comparison with 33 Xcc 
strains obtained from diff erent hosts and regions, whose allelic profi les had been determined 
previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profi le 
determination and Phyloviz software for constructing a minimum spanning tree. The obtained 
allelic profi le of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and 
rpoD genes, respectively. This profi le is assigned as sequence type 2 (ST2) and it coincides 
with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No 
connection between sequence type (ST) and the host was detected.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage",
pages = "26-19",
number = "1",
volume = "35",
doi = "10.2298/PIF2001019P"
}

Popović, T., Jelušić, A., Mitrović, P., Iličić, R.,& Marković, S.. (2020). Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 35(1), 19-26.
https://doi.org/10.2298/PIF2001019P

Popović T, Jelušić A, Mitrović P, Iličić R, Marković S. Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage. in Pesticidi i fitomedicina. 2020;35(1):19-26.
doi:10.2298/PIF2001019P .

Popović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Marković, Sanja, "Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage" in Pesticidi i fitomedicina, 35, no. 1 (2020):19-26,
https://doi.org/10.2298/PIF2001019P . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1935
AB  - Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or 
endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. 
Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight 
symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae
vegetables.
Objectives: Metagenomic approach was used to reveal differences in microbial communities 
colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial
blight symptoms and one healthy variety, all collected from five different localities in Vojvodina 
(Serbia) in 2018. 
Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA
sequences from five tested samples, and to gain insight into their alpha and beta diversity. 
Comparative taxonomic analysis of the obtained sequencing metadata was processed 
bioinformaticaly.
Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, 
Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. 
Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike 
healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the 
most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were 
present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in 
healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 
1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) 
in healthy oilseed rape variety.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape
SP  - 899
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1935
ER  - 

@conference{
author = "Jelušić, Aleksandra and Dimkić, Ivica and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or 
endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. 
Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight 
symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae
vegetables.
Objectives: Metagenomic approach was used to reveal differences in microbial communities 
colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial
blight symptoms and one healthy variety, all collected from five different localities in Vojvodina 
(Serbia) in 2018. 
Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA
sequences from five tested samples, and to gain insight into their alpha and beta diversity. 
Comparative taxonomic analysis of the obtained sequencing metadata was processed 
bioinformaticaly.
Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, 
Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. 
Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike 
healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the 
most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were 
present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in 
healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 
1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) 
in healthy oilseed rape variety.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape",
pages = "899",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1935"
}

Jelušić, A., Dimkić, I., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 899.
https://hdl.handle.net/21.15107/rcub_rimsi_1935

Jelušić A, Dimkić I, Berić T, Mitrović P, Marković S, Stanković S, Popović T. Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape. in 8th Congress of  European Microbiologists. 2019;:899.
https://hdl.handle.net/21.15107/rcub_rimsi_1935 .

Jelušić, Aleksandra, Dimkić, Ivica, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape" in 8th Congress of  European Microbiologists (2019):899,
https://hdl.handle.net/21.15107/rcub_rimsi_1935 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1912
AB  - Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant 
for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also 
for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can 
be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most 
significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) 
causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing 
bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from 
different localities in Serbia, with the aim to examine their genetic features, through 
sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified 
with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained 
sequences were checked for homology with strains available in NCBI database. Multilocus 
sequence analysis (MLSA) was then performed to determine relatedness among the tested 
isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing 
99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided
into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates
from this host. These results could be connected with pathogen adaptation to winter oilseed
rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable
crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria.
This observation on Serbian Xcc isolates gives a significant input on genetic variation and
constitutes a highly informative sample of X. campestris diversity.
PB  - Serbian Genetic Society,  Belgrade, Serbia
C3  - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
T1  - GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE
SP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1912
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant 
for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also 
for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can 
be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most 
significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) 
causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing 
bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from 
different localities in Serbia, with the aim to examine their genetic features, through 
sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified 
with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained 
sequences were checked for homology with strains available in NCBI database. Multilocus 
sequence analysis (MLSA) was then performed to determine relatedness among the tested 
isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing 
99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided
into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates
from this host. These results could be connected with pathogen adaptation to winter oilseed
rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable
crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria.
This observation on Serbian Xcc isolates gives a significant input on genetic variation and
constitutes a highly informative sample of X. campestris diversity.",
publisher = "Serbian Genetic Society,  Belgrade, Serbia",
journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY",
title = "GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE",
pages = "163",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1912"
}

Jelušić, A., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
Serbian Genetic Society,  Belgrade, Serbia., 163.
https://hdl.handle.net/21.15107/rcub_rimsi_1912

Jelušić A, Berić T, Mitrović P, Marković S, Stanković S, Popović T. GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:163.
https://hdl.handle.net/21.15107/rcub_rimsi_1912 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):163,
https://hdl.handle.net/21.15107/rcub_rimsi_1912 .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, Ivan
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1215
AB  - The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia
EP  - 1457
IS  - 8
SP  - 1448
VL  - 68
DO  - 10.1111/ppa.13064
ER  - 

@article{
author = "Popović, Tatjana and Mitrović, Petar and Jelušić, Aleksandra and Dimkić, Ivica and Marjanović-Jeromela, Ana and Nikolić, Ivan and Stanković, Slaviša",
year = "2019",
abstract = "The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia",
pages = "1457-1448",
number = "8",
volume = "68",
doi = "10.1111/ppa.13064"
}

Popović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanović-Jeromela, A., Nikolić, I.,& Stanković, S.. (2019). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology
Wiley, Hoboken., 68(8), 1448-1457.
https://doi.org/10.1111/ppa.13064

Popović T, Mitrović P, Jelušić A, Dimkić I, Marjanović-Jeromela A, Nikolić I, Stanković S. Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology. 2019;68(8):1448-1457.
doi:10.1111/ppa.13064 .

Popović, Tatjana, Mitrović, Petar, Jelušić, Aleksandra, Dimkić, Ivica, Marjanović-Jeromela, Ana, Nikolić, Ivan, Stanković, Slaviša, "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia" in Plant Pathology, 68, no. 8 (2019):1448-1457,
https://doi.org/10.1111/ppa.13064 . .