TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Marković, Sanja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1916
AB  - Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of 
cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which aff ect 
proper cabbage growth, leading to head weight and quality losses and thereby drastically 
reducing its marketing value. The pathogen is genetically diverse, which is evident from the 
presence of eleven races worldwide and more than thirty combinations of allelic profi les. 
Therefore, this study aimed to determine the allelic profi les of Serbian cabbage Xcc strains 
obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was fi rst 
amplifi ed using polymerase chain reaction (PCR) with four housekeeping genes – P-X dnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were fi nally used 
to determine allelic profi les. Allelic profi les were determined by comparison with 33 Xcc 
strains obtained from diff erent hosts and regions, whose allelic profi les had been determined 
previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profi le 
determination and Phyloviz software for constructing a minimum spanning tree. The obtained 
allelic profi le of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and 
rpoD genes, respectively. This profi le is assigned as sequence type 2 (ST2) and it coincides 
with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No 
connection between sequence type (ST) and the host was detected.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage
EP  - 26
IS  - 1
SP  - 19
VL  - 35
DO  - 10.2298/PIF2001019P
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Marković, Sanja",
year = "2020",
abstract = "Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of 
cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which aff ect 
proper cabbage growth, leading to head weight and quality losses and thereby drastically 
reducing its marketing value. The pathogen is genetically diverse, which is evident from the 
presence of eleven races worldwide and more than thirty combinations of allelic profi les. 
Therefore, this study aimed to determine the allelic profi les of Serbian cabbage Xcc strains 
obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was fi rst 
amplifi ed using polymerase chain reaction (PCR) with four housekeeping genes – P-X dnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were fi nally used 
to determine allelic profi les. Allelic profi les were determined by comparison with 33 Xcc 
strains obtained from diff erent hosts and regions, whose allelic profi les had been determined 
previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profi le 
determination and Phyloviz software for constructing a minimum spanning tree. The obtained 
allelic profi le of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and 
rpoD genes, respectively. This profi le is assigned as sequence type 2 (ST2) and it coincides 
with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No 
connection between sequence type (ST) and the host was detected.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage",
pages = "26-19",
number = "1",
volume = "35",
doi = "10.2298/PIF2001019P"
}

Popović, T., Jelušić, A., Mitrović, P., Iličić, R.,& Marković, S.. (2020). Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 35(1), 19-26.
https://doi.org/10.2298/PIF2001019P

Popović T, Jelušić A, Mitrović P, Iličić R, Marković S. Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage. in Pesticidi i fitomedicina. 2020;35(1):19-26.
doi:10.2298/PIF2001019P .

Popović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Marković, Sanja, "Allelic profi le of Serbian Xanthomonas campestris  pv. campestris isolates from cabbage" in Pesticidi i fitomedicina, 35, no. 1 (2020):19-26,
https://doi.org/10.2298/PIF2001019P . .