TY  - CONF
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Sedlarević Zorić, Ana
AU  - Jelušić, Aleksandra
AU  - Šušić, Nikola
AU  - Marković, Sanja
AU  - Veljović-Jovanović, Sonja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2058
AB  - Due to increased urbanization and industrialization, the emission of toxic material into the
atmosphere is in expansion, which has a negative impact on the environment and human health. In
this research, we monitored the effect of air pollution on the peroxidase (POD) activity and total
antioxidant capacity of different tree species during the growing season. The main goal was to
determine which tree species developed the highest tolerance to unfavorable environmental
conditions at the end of growing season, based on the response of their antioxidative metabolism. The
greatest change in POD activity was observed in the Fagus sylvatica L. leaves, where enzyme activity
was more than doubled in the autumn, in comparison to spring. On the other hand, decrease in POD
activity was the greatest in Cedrus atlantica (Endl.) Manetti ex Carrière needles. Moreover, total
antioxidative capacity was altered during growing season in almost all examined tree species. The
Magnolia spp. showed the most consistent response to the given environmental pollution with both
portrayed parameters induced during growing season. In general, we can conclude that the tree
species investigated in this research possess distinctive tolerance potential to air pollutants.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th International Conference Ecological Truth & Environmental Research
T1  - Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season
EP  - 134
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2058
ER  - 

@conference{
author = "Živanović, Bojana and Milić Komić, Sonja and Sedlarević Zorić, Ana and Jelušić, Aleksandra and Šušić, Nikola and Marković, Sanja and Veljović-Jovanović, Sonja",
year = "2023",
abstract = "Due to increased urbanization and industrialization, the emission of toxic material into the
atmosphere is in expansion, which has a negative impact on the environment and human health. In
this research, we monitored the effect of air pollution on the peroxidase (POD) activity and total
antioxidant capacity of different tree species during the growing season. The main goal was to
determine which tree species developed the highest tolerance to unfavorable environmental
conditions at the end of growing season, based on the response of their antioxidative metabolism. The
greatest change in POD activity was observed in the Fagus sylvatica L. leaves, where enzyme activity
was more than doubled in the autumn, in comparison to spring. On the other hand, decrease in POD
activity was the greatest in Cedrus atlantica (Endl.) Manetti ex Carrière needles. Moreover, total
antioxidative capacity was altered during growing season in almost all examined tree species. The
Magnolia spp. showed the most consistent response to the given environmental pollution with both
portrayed parameters induced during growing season. In general, we can conclude that the tree
species investigated in this research possess distinctive tolerance potential to air pollutants.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th International Conference Ecological Truth & Environmental Research",
title = "Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season",
pages = "134-129",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2058"
}

Živanović, B., Milić Komić, S., Sedlarević Zorić, A., Jelušić, A., Šušić, N., Marković, S.,& Veljović-Jovanović, S.. (2023). Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season. in 30th International Conference Ecological Truth & Environmental Research
University of Belgrade, Technical Faculty in Bor., 129-134.
https://hdl.handle.net/21.15107/rcub_rimsi_2058

Živanović B, Milić Komić S, Sedlarević Zorić A, Jelušić A, Šušić N, Marković S, Veljović-Jovanović S. Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season. in 30th International Conference Ecological Truth & Environmental Research. 2023;:129-134.
https://hdl.handle.net/21.15107/rcub_rimsi_2058 .

Živanović, Bojana, Milić Komić, Sonja, Sedlarević Zorić, Ana, Jelušić, Aleksandra, Šušić, Nikola, Marković, Sanja, Veljović-Jovanović, Sonja, "Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season" in 30th International Conference Ecological Truth & Environmental Research (2023):129-134,
https://hdl.handle.net/21.15107/rcub_rimsi_2058 .

TY  - CONF
AU  - Šušić, Nikola
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Sedlarević Zorić, Ana
AU  - Veljović-Jovanović, Sonja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1984
AB  - The first months are the most sensitive phase of oak seedling development. Light conditions have an important role in this sense, both from the physiological and management viewpoint. We investigated the response of pedunculate oak seedlings to three growth light intensities (100, 550 and 2000 μmol cm−2s−1) during development of the first and second growth flush. The low and high light intensities of PAR are supposed to mimic the effects of solar radiation under extreme natural conditions (closed canopy and open field). The response of seedlings to different light intensities was evaluated by determining the photochemical activity of photosystem II, leaf chlorophyll concentration and epidermal flavonoid accumulation for both growth flushes. At the end of the experiment (after 4.5 months) the effects of different treatments on growth parameters were also determined. We showed here that oak seedlings responded to varying light intensities by modifying their physiological and morphological traits of successive growth flushes. At medium light, seedlings had the highest PSII photochemical activity in the 2nd flush. High light induced very low photochemical efficiency of photosystem II in both growth flushes indicating the development of high non-photochemical quenching of chlorophyll fluorescence as part of photoprotective mechanism. In accordance with high photosynthetic yield at ML, the investment of photosynthates in growth, especially biomass allocation towards root system was confirmed. ML was optimal for seedling development in the first months. The results may contribute to a better understanding of oak seedling development and acclimation and could have importance for oak natural regeneration.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
T1  - ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION
EP  - 140
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1984
ER  - 

@conference{
author = "Šušić, Nikola and Milić Komić, Sonja and Živanović, Bojana and Jelušić, Aleksandra and Marković, Sanja and Sedlarević Zorić, Ana and Veljović-Jovanović, Sonja",
year = "2023",
abstract = "The first months are the most sensitive phase of oak seedling development. Light conditions have an important role in this sense, both from the physiological and management viewpoint. We investigated the response of pedunculate oak seedlings to three growth light intensities (100, 550 and 2000 μmol cm−2s−1) during development of the first and second growth flush. The low and high light intensities of PAR are supposed to mimic the effects of solar radiation under extreme natural conditions (closed canopy and open field). The response of seedlings to different light intensities was evaluated by determining the photochemical activity of photosystem II, leaf chlorophyll concentration and epidermal flavonoid accumulation for both growth flushes. At the end of the experiment (after 4.5 months) the effects of different treatments on growth parameters were also determined. We showed here that oak seedlings responded to varying light intensities by modifying their physiological and morphological traits of successive growth flushes. At medium light, seedlings had the highest PSII photochemical activity in the 2nd flush. High light induced very low photochemical efficiency of photosystem II in both growth flushes indicating the development of high non-photochemical quenching of chlorophyll fluorescence as part of photoprotective mechanism. In accordance with high photosynthetic yield at ML, the investment of photosynthates in growth, especially biomass allocation towards root system was confirmed. ML was optimal for seedling development in the first months. The results may contribute to a better understanding of oak seedling development and acclimation and could have importance for oak natural regeneration.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23",
title = "ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION",
pages = "140-135",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1984"
}

Šušić, N., Milić Komić, S., Živanović, B., Jelušić, A., Marković, S., Sedlarević Zorić, A.,& Veljović-Jovanović, S.. (2023). ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
University of Belgrade, Technical Faculty in Bor., 135-140.
https://hdl.handle.net/21.15107/rcub_rimsi_1984

Šušić N, Milić Komić S, Živanović B, Jelušić A, Marković S, Sedlarević Zorić A, Veljović-Jovanović S. ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23. 2023;:135-140.
https://hdl.handle.net/21.15107/rcub_rimsi_1984 .

Šušić, Nikola, Milić Komić, Sonja, Živanović, Bojana, Jelušić, Aleksandra, Marković, Sanja, Sedlarević Zorić, Ana, Veljović-Jovanović, Sonja, "ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION" in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23 (2023):135-140,
https://hdl.handle.net/21.15107/rcub_rimsi_1984 .

TY  - CONF
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Sedlarević Zorić, Ana
AU  - Šušić, Nikola
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1983
AB  - Street trees are important component of urban forest presenting a first barrier between air pollution
originated from vehicle traffic and pedestrians. It implies that an improvement of air quality in urban
areas greatly depends on green biomass, in short, the bigger and greener tree crown, better for human
health and wellbeing. Determination of Leaf Nitrogen Balance Index (LNBI) and chlorophyll
concentration (ChlC) by a non-invasive methodology and a user friendly instrument (Dualex 4,
Force), widely used in agronomy and horticulture, was tested here for the assessment of tree fitness in
urban zones. Investment of energy and resources either in growth or defence according to the tradeoff
strategy of plants may be indicated by LNBI, which approximately presents a Nitrogen/Carbon
ratio. We selected few tree species from Belgrade’s streets to determine those two parameters during
summer. We also presented the changes in those parameters of the introduced bamboo species within
ten years at several urban locations in Belgrade differing in air pollution aiming to evaluate
usefulness the LNBI parameter in access of multiyear exposure to the effect of intense vehicle traffic.
Numerous limiting factors for development of healthy tree crowns in urban ecosystem, such as low
capacity to cope with toxic pollutant, sensitivity to diseases, early senescence and etc., greatly depends
on tree species. We propose this methodology may also contribute in the process of choice of the
adequate tree species to be planted along streets.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
T1  - LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE
EP  - 128
SP  - 122
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1983
ER  - 

@conference{
author = "Veljović-Jovanović, Sonja and Milić Komić, Sonja and Živanović, Bojana and Sedlarević Zorić, Ana and Šušić, Nikola",
year = "2023",
abstract = "Street trees are important component of urban forest presenting a first barrier between air pollution
originated from vehicle traffic and pedestrians. It implies that an improvement of air quality in urban
areas greatly depends on green biomass, in short, the bigger and greener tree crown, better for human
health and wellbeing. Determination of Leaf Nitrogen Balance Index (LNBI) and chlorophyll
concentration (ChlC) by a non-invasive methodology and a user friendly instrument (Dualex 4,
Force), widely used in agronomy and horticulture, was tested here for the assessment of tree fitness in
urban zones. Investment of energy and resources either in growth or defence according to the tradeoff
strategy of plants may be indicated by LNBI, which approximately presents a Nitrogen/Carbon
ratio. We selected few tree species from Belgrade’s streets to determine those two parameters during
summer. We also presented the changes in those parameters of the introduced bamboo species within
ten years at several urban locations in Belgrade differing in air pollution aiming to evaluate
usefulness the LNBI parameter in access of multiyear exposure to the effect of intense vehicle traffic.
Numerous limiting factors for development of healthy tree crowns in urban ecosystem, such as low
capacity to cope with toxic pollutant, sensitivity to diseases, early senescence and etc., greatly depends
on tree species. We propose this methodology may also contribute in the process of choice of the
adequate tree species to be planted along streets.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23",
title = "LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE",
pages = "128-122",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1983"
}

Veljović-Jovanović, S., Milić Komić, S., Živanović, B., Sedlarević Zorić, A.,& Šušić, N.. (2023). LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
University of Belgrade, Technical Faculty in Bor., 122-128.
https://hdl.handle.net/21.15107/rcub_rimsi_1983

Veljović-Jovanović S, Milić Komić S, Živanović B, Sedlarević Zorić A, Šušić N. LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23. 2023;:122-128.
https://hdl.handle.net/21.15107/rcub_rimsi_1983 .

Veljović-Jovanović, Sonja, Milić Komić, Sonja, Živanović, Bojana, Sedlarević Zorić, Ana, Šušić, Nikola, "LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE" in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23 (2023):122-128,
https://hdl.handle.net/21.15107/rcub_rimsi_1983 .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Dumanović, Jelena
AU  - kolarž, Predrag
AU  - Sedlarević Zorić, Ana
AU  - Morina, Filis
AU  - Vidović, Marija
AU  - Veljović-Jovanović, Sonja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2220
AB  - Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum x citriodorum, and Ocimum
basilicum var. purpurascens) were grown under moderate light (about 300 umol photons m-2 s-1)
in a glasshouse or growth chamber and then either transferred to an open field (average daily dose:
29.2 kJ m-2 d-1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m-2 d-1),
to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity
(TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine
the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation
at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially
caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a
two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced
preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity,
but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal
flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition
of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated
with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological
changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance
mechanism to high doses of UV-B irradiation.
PB  - MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005
T2  - International Journal of Molecular Sciences
T1  - Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties
VL  - 24(20), 15350
DO  - 10.3390/ijms242015350
ER  - 

@article{
author = "Milić Komić, Sonja and Živanović, Bojana and Dumanović, Jelena and kolarž, Predrag and Sedlarević Zorić, Ana and Morina, Filis and Vidović, Marija and Veljović-Jovanović, Sonja",
year = "2023",
abstract = "Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum x citriodorum, and Ocimum
basilicum var. purpurascens) were grown under moderate light (about 300 umol photons m-2 s-1)
in a glasshouse or growth chamber and then either transferred to an open field (average daily dose:
29.2 kJ m-2 d-1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m-2 d-1),
to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity
(TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine
the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation
at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially
caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a
two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced
preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity,
but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal
flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition
of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated
with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological
changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance
mechanism to high doses of UV-B irradiation.",
publisher = "MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005",
journal = "International Journal of Molecular Sciences",
title = "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties",
volume = "24(20), 15350",
doi = "10.3390/ijms242015350"
}

Milić Komić, S., Živanović, B., Dumanović, J., kolarž, P., Sedlarević Zorić, A., Morina, F., Vidović, M.,& Veljović-Jovanović, S.. (2023). Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences
MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005., 24(20), 15350.
https://doi.org/10.3390/ijms242015350

Milić Komić S, Živanović B, Dumanović J, kolarž P, Sedlarević Zorić A, Morina F, Vidović M, Veljović-Jovanović S. Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences. 2023;24(20), 15350.
doi:10.3390/ijms242015350 .

Milić Komić, Sonja, Živanović, Bojana, Dumanović, Jelena, kolarž, Predrag, Sedlarević Zorić, Ana, Morina, Filis, Vidović, Marija, Veljović-Jovanović, Sonja, "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties" in International Journal of Molecular Sciences, 24(20), 15350 (2023),
https://doi.org/10.3390/ijms242015350 . .

TY  - CONF
AU  - Pantelic, Ana
AU  - Stevanovic, Strahinja
AU  - Milić Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3087
AB  - Ramonda serbica Panc. is an ancient resurrection plant, that survives a long desiccation period and fully recovers metabolic functions upon watering. The main characteristic of desiccationtolerant plant species is their ability to accumulate protective late embryogenesis abounded protein (LEAPs). To propose their role in R. serbica desiccation tolerance we structurally analysed LEAPs in hydrated and desiccated leaves. According to transcriptomics, 318 LEAPs were identified and classified into seven family groups based on protein BLAST analysis and conserved motifs (Pfam). The largest LEAPs belonged to the LEA2 and LEA4 protein family groups. We employed online tools to analyse physicochemical characteristics (Expasy, ProtParam, BioPython, GRAVY calculator), disorder propensity, and characterization protein structures (FELLS, JPred, SOPMA, PsiPred, Phyre2, Espritz-DisProt, Espritz-X, Iupred, TMHMM, +Heliquest). The most abundant, atypical LEA2 group containing 127, mostly hydrophobic proteins, was divided into five subgroups. Members of this group were predicted to fold into globular domains, β-barrel at the C-terminus, followed by transmembrane hydrophobic-helices and disordered N-terminal regions. Results indicated the possible involvement in the protection of the chloroplastic membranes. The LEA4 group exhibited an exceptionally high tendency to form amphipathic α-helices and simultaneously had a high disorder propensity. This group is made of 96 proteins, classified into 3 subgroups. The high content of polar and charged amino acids (lysine, glutamate, and aspartate) is characteristic of this group. Motifs corresponding to the R. serbica LEA4 protein family group folded into A-type α-helices that contained positive, negative, and hydrophobic surfaces. Based on previous knowledge, the possible functions of the LEA2 and LEA4 groups are discussed with significant implications on cell preservation technology and the improvement of crop drought tolerance.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference, 2023, 4, 68-68
T1  - Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.
SP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3087
ER  - 

@conference{
author = "Pantelic, Ana and Stevanovic, Strahinja and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2023",
abstract = "Ramonda serbica Panc. is an ancient resurrection plant, that survives a long desiccation period and fully recovers metabolic functions upon watering. The main characteristic of desiccationtolerant plant species is their ability to accumulate protective late embryogenesis abounded protein (LEAPs). To propose their role in R. serbica desiccation tolerance we structurally analysed LEAPs in hydrated and desiccated leaves. According to transcriptomics, 318 LEAPs were identified and classified into seven family groups based on protein BLAST analysis and conserved motifs (Pfam). The largest LEAPs belonged to the LEA2 and LEA4 protein family groups. We employed online tools to analyse physicochemical characteristics (Expasy, ProtParam, BioPython, GRAVY calculator), disorder propensity, and characterization protein structures (FELLS, JPred, SOPMA, PsiPred, Phyre2, Espritz-DisProt, Espritz-X, Iupred, TMHMM, +Heliquest). The most abundant, atypical LEA2 group containing 127, mostly hydrophobic proteins, was divided into five subgroups. Members of this group were predicted to fold into globular domains, β-barrel at the C-terminus, followed by transmembrane hydrophobic-helices and disordered N-terminal regions. Results indicated the possible involvement in the protection of the chloroplastic membranes. The LEA4 group exhibited an exceptionally high tendency to form amphipathic α-helices and simultaneously had a high disorder propensity. This group is made of 96 proteins, classified into 3 subgroups. The high content of polar and charged amino acids (lysine, glutamate, and aspartate) is characteristic of this group. Motifs corresponding to the R. serbica LEA4 protein family group folded into A-type α-helices that contained positive, negative, and hydrophobic surfaces. Based on previous knowledge, the possible functions of the LEA2 and LEA4 groups are discussed with significant implications on cell preservation technology and the improvement of crop drought tolerance.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference, 2023, 4, 68-68",
title = "Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.",
pages = "68",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3087"
}

Pantelic, A., Stevanovic, S., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2023). Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference, 2023, 4, 68-68
Belgrade : Institute of molecular genetics and genetic engineering., 68.
https://hdl.handle.net/21.15107/rcub_rimsi_3087

Pantelic A, Stevanovic S, Milić Komić S, Kilibarda N, Vidović M. Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference, 2023, 4, 68-68. 2023;:68.
https://hdl.handle.net/21.15107/rcub_rimsi_3087 .

Pantelic, Ana, Stevanovic, Strahinja, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc." in 4th Belgrade Bioinformatics Conference, 2023, 4, 68-68 (2023):68,
https://hdl.handle.net/21.15107/rcub_rimsi_3087 .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Veljović-Jovanović, Sonja
AU  - Pantelic, Ana
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2900
AB  - Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica Panč. is a relic and endemic species that belongs to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing subject of many researchers. Most of the genes responsible for this amazing ability are present in other plants, and research of those genes which could be activated in crops is growing much more attention because of the imminent crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation tolerance. Defining LEAPs physicochemical characteristics and specific physiological functions may lead us to their applicability in other areas of research.
PB  - Novi Sad : Faculty of Sciences, Department of Biology
T2  - Biologia Serbica
T1  - Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.
EP  - 66
IS  - 44
SP  - 59
VL  - 1
DO  - 10.5281/zenodo.7075212
ER  - 

@article{
author = "Milić Komić, Sonja and Veljović-Jovanović, Sonja and Pantelic, Ana and Vidović, Marija",
year = "2022",
abstract = "Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica Panč. is a relic and endemic species that belongs to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing subject of many researchers. Most of the genes responsible for this amazing ability are present in other plants, and research of those genes which could be activated in crops is growing much more attention because of the imminent crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation tolerance. Defining LEAPs physicochemical characteristics and specific physiological functions may lead us to their applicability in other areas of research.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology",
journal = "Biologia Serbica",
title = "Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.",
pages = "66-59",
number = "44",
volume = "1",
doi = "10.5281/zenodo.7075212"
}

Milić Komić, S., Veljović-Jovanović, S., Pantelic, A.,& Vidović, M.. (2022). Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology., 1(44), 59-66.
https://doi.org/10.5281/zenodo.7075212

Milić Komić S, Veljović-Jovanović S, Pantelic A, Vidović M. Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.. in Biologia Serbica. 2022;1(44):59-66.
doi:10.5281/zenodo.7075212 .

Milić Komić, Sonja, Veljović-Jovanović, Sonja, Pantelic, Ana, Vidović, Marija, "Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč." in Biologia Serbica, 1, no. 44 (2022):59-66,
https://doi.org/10.5281/zenodo.7075212 . .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Veljović-Jovanović, Sonja
AU  - Pantelic, Ana
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3086
AB  - Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica is a relic and endemic species that belong to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing subject to many researchers. Most of the genes responsible for this amazing ability are present in other plants, and this path of research where those genes could be activated in crops is growing much more attention because of the imminent crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation tolerance. Defining physicochemical characteristics and specific physiological functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability in other areas of research.
PB  - Belgrade : Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"
T1  - Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3086
ER  - 

@conference{
author = "Milić Komić, Sonja and Veljović-Jovanović, Sonja and Pantelic, Ana and Vidović, Marija",
year = "2022",
abstract = "Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica is a relic and endemic species that belong to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing subject to many researchers. Most of the genes responsible for this amazing ability are present in other plants, and this path of research where those genes could be activated in crops is growing much more attention because of the imminent crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation tolerance. Defining physicochemical characteristics and specific physiological functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability in other areas of research.",
publisher = "Belgrade : Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"",
title = "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein",
pages = "37",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3086"
}

Milić Komić, S., Veljović-Jovanović, S., Pantelic, A.,& Vidović, M.. (2022). Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"
Belgrade : Faculty of Chemistry, Serbian Biochemical Society., 37.
https://hdl.handle.net/21.15107/rcub_rimsi_3086

Milić Komić S, Veljović-Jovanović S, Pantelic A, Vidović M. Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry". 2022;:37.
https://hdl.handle.net/21.15107/rcub_rimsi_3086 .

Milić Komić, Sonja, Veljović-Jovanović, Sonja, Pantelic, Ana, Vidović, Marija, "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein" in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry" (2022):37,
https://hdl.handle.net/21.15107/rcub_rimsi_3086 .

TY  - CONF
AU  - Pantelic, Ana
AU  - Stevanovic, Strahinja
AU  - Milić, Dejana
AU  - Milić Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3085
AB  - An ancient resurrection plant Ramonda serbica Panc. is able to survive a long desiccation period and reestablish metabolic activity upon watering. A hallmark of desiccation tolerance in the resurrection species is the accumulation of protective late embryogenesis abundant proteins (LEAPs). These intrinsically disordered proteins (IDPs) may stabilize the correct structure of proteins and membranes during cellular dehydration. The aim of our study was to assess LEA genes’ expression levels in hydrated (HL) and desiccated leaves (DL) and to identify, characterise, and estimate the potential role of R. serbica LEAPs in desiccation tolerance. In total, 318 LEAPs from HL and DL were identified and classified into the seven LEA protein family groups ranging from LEA1-LEA5, seed maturation proteins (SMPs), and dehydrins (DEH). Analysis of the physicochemical properties, motif architecture, secondary structure, homology, and phylogenetic relationships demonstrated that R. serbica LEAPs greatly differed among the LEA family groups. The most abundant LEA2 proteins (mostly downregulated upon desiccation) exhibited lower hydrophilicity and propensity to fold into organised globular domains. Oppositely, hydrophilic LEA4 proteins tended to form amphipathic, A-type, α-helices. Most of desiccation-upregulated LEA genes encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other ID LEAPs (e.g. LEA1, LEA3, LEA4) might participate in forming intracellular proteinaceous condensates or adopt amphipathic α-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. Taken together, possible functions of LEAPs are discussed with significant implications on drought tolerance improvement of crops grown in arid areas.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83-
T1  - Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization
SP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3085
ER  - 

@conference{
author = "Pantelic, Ana and Stevanovic, Strahinja and Milić, Dejana and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2022",
abstract = "An ancient resurrection plant Ramonda serbica Panc. is able to survive a long desiccation period and reestablish metabolic activity upon watering. A hallmark of desiccation tolerance in the resurrection species is the accumulation of protective late embryogenesis abundant proteins (LEAPs). These intrinsically disordered proteins (IDPs) may stabilize the correct structure of proteins and membranes during cellular dehydration. The aim of our study was to assess LEA genes’ expression levels in hydrated (HL) and desiccated leaves (DL) and to identify, characterise, and estimate the potential role of R. serbica LEAPs in desiccation tolerance. In total, 318 LEAPs from HL and DL were identified and classified into the seven LEA protein family groups ranging from LEA1-LEA5, seed maturation proteins (SMPs), and dehydrins (DEH). Analysis of the physicochemical properties, motif architecture, secondary structure, homology, and phylogenetic relationships demonstrated that R. serbica LEAPs greatly differed among the LEA family groups. The most abundant LEA2 proteins (mostly downregulated upon desiccation) exhibited lower hydrophilicity and propensity to fold into organised globular domains. Oppositely, hydrophilic LEA4 proteins tended to form amphipathic, A-type, α-helices. Most of desiccation-upregulated LEA genes encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other ID LEAPs (e.g. LEA1, LEA3, LEA4) might participate in forming intracellular proteinaceous condensates or adopt amphipathic α-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. Taken together, possible functions of LEAPs are discussed with significant implications on drought tolerance improvement of crops grown in arid areas.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83-",
title = "Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization",
pages = "95",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3085"
}

Pantelic, A., Stevanovic, S., Milić, D., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2022). Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83-
Serbian Plant Physiology Society., 95.
https://hdl.handle.net/21.15107/rcub_rimsi_3085

Pantelic A, Stevanovic S, Milić D, Milić Komić S, Kilibarda N, Vidović M. Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83-. 2022;:95.
https://hdl.handle.net/21.15107/rcub_rimsi_3085 .

Pantelic, Ana, Stevanovic, Strahinja, Milić, Dejana, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization" in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83- (2022):95,
https://hdl.handle.net/21.15107/rcub_rimsi_3085 .

TY  - CONF
AU  - Živanović, Bojana
AU  - Prokić, Ljiljana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1871
AB  - The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1871
ER  - 

@conference{
author = "Živanović, Bojana and Prokić, Ljiljana and Milić Komić, Sonja and Nikolić, Nenad and Sedlarević Zorić, Ana and Vidović, Marija and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1871"
}

Živanović, B., Prokić, L., Milić Komić, S., Nikolić, N., Sedlarević Zorić, A., Vidović, M.,& Veljović-Jovanović, S.. (2022). Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Serbian Plant Physiology Society., 83.
https://hdl.handle.net/21.15107/rcub_rimsi_1871

Živanović B, Prokić L, Milić Komić S, Nikolić N, Sedlarević Zorić A, Vidović M, Veljović-Jovanović S. Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;:83.
https://hdl.handle.net/21.15107/rcub_rimsi_1871 .

Živanović, Bojana, Prokić, Ljiljana, Milić Komić, Sonja, Nikolić, Nenad, Sedlarević Zorić, Ana, Vidović, Marija, Veljović-Jovanović, Sonja, "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca" in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022):83,
https://hdl.handle.net/21.15107/rcub_rimsi_1871 .

TY  - CONF
AU  - Golob, Aleksandra
AU  - Ojdanič, Nik
AU  - Živanović, Bojana
AU  - Germ, Mateja
AU  - Milić Komić, Sonja
AU  - Sedlarević Zorić, Ana
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Mavrič Čermelj, Anja
AU  - Samardžić, Jelena
AU  - Veljović-Jovanović, Sonja
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1872
AB  - Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered a
beneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperatures
by promoting antioxidant production. However, the underlying mechanisms are still not
elucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,
leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed to
water shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;
(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impacts
photochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflective
and transmissive properties. Si application between flag leaf emergence and grain filling
had the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was the
most abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was the
only hydroxycinnamic acid showing significant accumulation with the latest applied Si compared
to no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,
aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to drought
without Si addition, while lysine was the most accumulated in the leaves supplemented by Si at
the grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to drought
regardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, its
effect was dependent on the barley growth phase prior to supplementation.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought
SP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1872
ER  - 

@conference{
author = "Golob, Aleksandra and Ojdanič, Nik and Živanović, Bojana and Germ, Mateja and Milić Komić, Sonja and Sedlarević Zorić, Ana and Milić, Dejana and Pantelić, Ana and Mavrič Čermelj, Anja and Samardžić, Jelena and Veljović-Jovanović, Sonja and Vidović, Marija",
year = "2022",
abstract = "Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered a
beneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperatures
by promoting antioxidant production. However, the underlying mechanisms are still not
elucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,
leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed to
water shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;
(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impacts
photochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflective
and transmissive properties. Si application between flag leaf emergence and grain filling
had the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was the
most abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was the
only hydroxycinnamic acid showing significant accumulation with the latest applied Si compared
to no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,
aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to drought
without Si addition, while lysine was the most accumulated in the leaves supplemented by Si at
the grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to drought
regardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, its
effect was dependent on the barley growth phase prior to supplementation.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought",
pages = "67",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1872"
}

Golob, A., Ojdanič, N., Živanović, B., Germ, M., Milić Komić, S., Sedlarević Zorić, A., Milić, D., Pantelić, A., Mavrič Čermelj, A., Samardžić, J., Veljović-Jovanović, S.,& Vidović, M.. (2022). The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Serbian Plant Physiology Society., 67.
https://hdl.handle.net/21.15107/rcub_rimsi_1872

Golob A, Ojdanič N, Živanović B, Germ M, Milić Komić S, Sedlarević Zorić A, Milić D, Pantelić A, Mavrič Čermelj A, Samardžić J, Veljović-Jovanović S, Vidović M. The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;:67.
https://hdl.handle.net/21.15107/rcub_rimsi_1872 .

Golob, Aleksandra, Ojdanič, Nik, Živanović, Bojana, Germ, Mateja, Milić Komić, Sonja, Sedlarević Zorić, Ana, Milić, Dejana, Pantelić, Ana, Mavrič Čermelj, Anja, Samardžić, Jelena, Veljović-Jovanović, Sonja, Vidović, Marija, "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought" in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022):67,
https://hdl.handle.net/21.15107/rcub_rimsi_1872 .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1873
AB  - Three varieties of basil (Ocimum x citriodorum, Ocimum basilicum var. Genovese and Ocimum
basilicum var. purpurascens) were used to examine the effect of different PAR intensities (100, 400
and 1400 µmol photons m-2s-1), as well as different UV-B/PAR ratios, on leaf phenolics accumulation, and components of the ascorbate-glutathione cycle. Phenolic compounds represent the
most abundant class of secondary metabolites, and their function in plants involves protection
against numerous abiotic stresses, role in growth and development, flowering, reproduction and
seed dispersion. A preferential accumulation of HCAs over Flav, induction of class III POD activity
and decreased ascorbate content were characteristic responses to pro-oxidative effect of the high
UV-B/PAR ratio in all three basil varieties. The most remarkable result was the lack of accumulation
of epidermal UV absorbing substances, which appeared to be a crucial photoprotective mechanism in sunlight. The contrasting effect of ecologically relevant UV-B radiation on basil plants,
pro-oxidative vs. acclimative, was determined by the quality and intensity of the background light.
Acclimation of basil to high light comprises a number of processes, among which are the accumulation of epidermal flavonoids and total leaf phenolics, antioxidant response (increase in Asc and
GSH) and lack of downregulation of PSII upon increasing light intensity. Among varieties that we
used as model system in this study, purple basil, with the highest constitutive amount of anthocyanidins, showed the least induction of epidermal flavonoids and lack of light effect upon transfer
from initial 50 to 250 µmol photons m-2s-1.
PB  - Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology (23rd SPPS Meeting)
T1  - Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1873
ER  - 

@conference{
author = "Milić Komić, Sonja and Živanović, Bojana and Sedlarević Zorić, Ana and Vidović, Marija and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "Three varieties of basil (Ocimum x citriodorum, Ocimum basilicum var. Genovese and Ocimum
basilicum var. purpurascens) were used to examine the effect of different PAR intensities (100, 400
and 1400 µmol photons m-2s-1), as well as different UV-B/PAR ratios, on leaf phenolics accumulation, and components of the ascorbate-glutathione cycle. Phenolic compounds represent the
most abundant class of secondary metabolites, and their function in plants involves protection
against numerous abiotic stresses, role in growth and development, flowering, reproduction and
seed dispersion. A preferential accumulation of HCAs over Flav, induction of class III POD activity
and decreased ascorbate content were characteristic responses to pro-oxidative effect of the high
UV-B/PAR ratio in all three basil varieties. The most remarkable result was the lack of accumulation
of epidermal UV absorbing substances, which appeared to be a crucial photoprotective mechanism in sunlight. The contrasting effect of ecologically relevant UV-B radiation on basil plants,
pro-oxidative vs. acclimative, was determined by the quality and intensity of the background light.
Acclimation of basil to high light comprises a number of processes, among which are the accumulation of epidermal flavonoids and total leaf phenolics, antioxidant response (increase in Asc and
GSH) and lack of downregulation of PSII upon increasing light intensity. Among varieties that we
used as model system in this study, purple basil, with the highest constitutive amount of anthocyanidins, showed the least induction of epidermal flavonoids and lack of light effect upon transfer
from initial 50 to 250 µmol photons m-2s-1.",
publisher = "Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology (23rd SPPS Meeting)",
title = "Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1873"
}

Milić Komić, S., Živanović, B., Sedlarević Zorić, A., Vidović, M.,& Veljović-Jovanović, S.. (2022). Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties. in 4th International Conference on Plant Biology (23rd SPPS Meeting)
Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_rimsi_1873

Milić Komić S, Živanović B, Sedlarević Zorić A, Vidović M, Veljović-Jovanović S. Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties. in 4th International Conference on Plant Biology (23rd SPPS Meeting). 2022;:43.
https://hdl.handle.net/21.15107/rcub_rimsi_1873 .

Milić Komić, Sonja, Živanović, Bojana, Sedlarević Zorić, Ana, Vidović, Marija, Veljović-Jovanović, Sonja, "Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties" in 4th International Conference on Plant Biology (23rd SPPS Meeting) (2022):43,
https://hdl.handle.net/21.15107/rcub_rimsi_1873 .

TY  - JOUR
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Milić Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1575
AB  - Ramonda serbica Panc. is an ancient resurrection plant able to survive a long desiccation period and recover metabolic functions upon watering. The accumulation of protective late embryogenesis abundant proteins (LEAPs) is a desiccation tolerance hallmark. To propose their role in R. serbica desiccation tolerance, we structurally characterised LEAPs and evaluated LEA gene expression levels in hydrated and desiccated leaves. By integrating de novo transcriptomics and homologues LEAP domains, 318 R. serbica LEAPs were identified and classified according to their conserved motifs and phylogeny. The in silico analysis revealed that hydrophilic LEA4 proteins exhibited an exceptionally high tendency to form amphipathic α‐helices. The most abundant, atypical LEA2 group contained more hydrophobic proteins predicted to fold into the defined globular domains. Within the desiccation‐upregulated LEA genes, the majority encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins, while the greatest portion of downregulated genes encoded LEA2.3 and LEA2.5 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other intrinsically disordered LEAPs might participate in forming intracellular proteinaceous condensates or adopt amphipathic α‐helical conformation, enabling them to stabilise desiccation‐sensitive proteins and membranes. This comprehensive LEAPs structural characterisation is essential to understanding their function and regulation during desiccation aiming at crop drought tolerance improvement.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc.
IS  - 7
SP  - 3547
VL  - 23
DO  - 10.3390/ijms23073547
ER  - 

@article{
author = "Pantelić, Ana and Stevanović, Strahinja and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2022",
abstract = "Ramonda serbica Panc. is an ancient resurrection plant able to survive a long desiccation period and recover metabolic functions upon watering. The accumulation of protective late embryogenesis abundant proteins (LEAPs) is a desiccation tolerance hallmark. To propose their role in R. serbica desiccation tolerance, we structurally characterised LEAPs and evaluated LEA gene expression levels in hydrated and desiccated leaves. By integrating de novo transcriptomics and homologues LEAP domains, 318 R. serbica LEAPs were identified and classified according to their conserved motifs and phylogeny. The in silico analysis revealed that hydrophilic LEA4 proteins exhibited an exceptionally high tendency to form amphipathic α‐helices. The most abundant, atypical LEA2 group contained more hydrophobic proteins predicted to fold into the defined globular domains. Within the desiccation‐upregulated LEA genes, the majority encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins, while the greatest portion of downregulated genes encoded LEA2.3 and LEA2.5 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other intrinsically disordered LEAPs might participate in forming intracellular proteinaceous condensates or adopt amphipathic α‐helical conformation, enabling them to stabilise desiccation‐sensitive proteins and membranes. This comprehensive LEAPs structural characterisation is essential to understanding their function and regulation during desiccation aiming at crop drought tolerance improvement.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc.",
number = "7",
pages = "3547",
volume = "23",
doi = "10.3390/ijms23073547"
}

Pantelić, A., Stevanović, S., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2022). In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc.. in International Journal of Molecular Sciences
MDPI., 23(7), 3547.
https://doi.org/10.3390/ijms23073547

Pantelić A, Stevanović S, Milić Komić S, Kilibarda N, Vidović M. In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc.. in International Journal of Molecular Sciences. 2022;23(7):3547.
doi:10.3390/ijms23073547 .

Pantelić, Ana, Stevanović, Strahinja, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc." in International Journal of Molecular Sciences, 23, no. 7 (2022):3547,
https://doi.org/10.3390/ijms23073547 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Bagi, Ferenc
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1542
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia
IS  - 1
VL  - 106
DO  - 10.1094/PDIS-06-21-1128-PDN
ER  - 

@article{
author = "Marković, Sanja and Milić Komić, Sonja and Jelušić, Aleksandra and Ilicic, Renata and Bagi, Ferenc and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia",
number = "1",
volume = "106",
doi = "10.1094/PDIS-06-21-1128-PDN"
}

Marković, S., Milić Komić, S., Jelušić, A., Ilicic, R., Bagi, F., Stanković, S.,& Popović, T.. (2022). First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 106(1).
https://doi.org/10.1094/PDIS-06-21-1128-PDN

Marković S, Milić Komić S, Jelušić A, Ilicic R, Bagi F, Stanković S, Popović T. First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease. 2022;106(1).
doi:10.1094/PDIS-06-21-1128-PDN .

Marković, Sanja, Milić Komić, Sonja, Jelušić, Aleksandra, Ilicic, Renata, Bagi, Ferenc, Stanković, Slaviša, Popović, Tatjana, "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia" in Plant Disease, 106, no. 1 (2022),
https://doi.org/10.1094/PDIS-06-21-1128-PDN . .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Stevanović, Strahinja
AU  - Vidović, Marija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1491
AB  - Ramonda serbica Panc. is a resurrection plant that can survive long desiccation
periods (extreme loss of cellular water). The accumulation of late embryogenesis abundant
proteins (LEAPs) is a crucial step in desiccation tolerance mechanism. Based on in vitro studies,
LEAPs can be involved in antioxidative defense, ion sequestration, structural stabilization
of both membranes and enzymes during freezing or drying, while by forming intracellular
proteinaceous condensates they increase structural integrity and intracellular viscosity of cells
during desiccation. Here we investigated the antioxidative potential of LEAPs identified by de
novo transcriptomics of R. serbica, based on their primary and secondary confirmation. In our
previous work [1], we displayed the antioxidative capacity of 20 free proteogenic amino acids
(FAA) through determining their hydroxyl radical (•
OH, generated in Fenton reaction) scavenging
rate by using electron paramagnetic resonance. These results served as a basis for generating
a model for prediction of •
OH scavenging activity for selected proteins. In addition, the model
was built based on protein primary sequences, hydrophobicity, 3D structure and predicted
solvent accessible area. Manually curated data for peptides and proteins with experimentally
determined •
OH scavenging rate were used for training and testing. The model was fed into
machine learning algorithm and •
OH scavenging potential scale was created using IC50 values.
By applying our model, we classified 164 LEAPs according to their potential for •
OH scavenging.
Further work will focus on the experimental evaluation of the obtained model by measuring of
the rate of •
OH scavenging in the presence of recombinantly produced LEAPs.
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach
VL  - 177
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1491
ER  - 

@conference{
author = "Milić Komić, Sonja and Stevanović, Strahinja and Vidović, Marija",
year = "2021",
abstract = "Ramonda serbica Panc. is a resurrection plant that can survive long desiccation
periods (extreme loss of cellular water). The accumulation of late embryogenesis abundant
proteins (LEAPs) is a crucial step in desiccation tolerance mechanism. Based on in vitro studies,
LEAPs can be involved in antioxidative defense, ion sequestration, structural stabilization
of both membranes and enzymes during freezing or drying, while by forming intracellular
proteinaceous condensates they increase structural integrity and intracellular viscosity of cells
during desiccation. Here we investigated the antioxidative potential of LEAPs identified by de
novo transcriptomics of R. serbica, based on their primary and secondary confirmation. In our
previous work [1], we displayed the antioxidative capacity of 20 free proteogenic amino acids
(FAA) through determining their hydroxyl radical (•
OH, generated in Fenton reaction) scavenging
rate by using electron paramagnetic resonance. These results served as a basis for generating
a model for prediction of •
OH scavenging activity for selected proteins. In addition, the model
was built based on protein primary sequences, hydrophobicity, 3D structure and predicted
solvent accessible area. Manually curated data for peptides and proteins with experimentally
determined •
OH scavenging rate were used for training and testing. The model was fed into
machine learning algorithm and •
OH scavenging potential scale was created using IC50 values.
By applying our model, we classified 164 LEAPs according to their potential for •
OH scavenging.
Further work will focus on the experimental evaluation of the obtained model by measuring of
the rate of •
OH scavenging in the presence of recombinantly produced LEAPs.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach",
volume = "177",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1491"
}

Milić Komić, S., Stevanović, S.,& Vidović, M.. (2021). Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 177.
https://hdl.handle.net/21.15107/rcub_rimsi_1491

Milić Komić S, Stevanović S, Vidović M. Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach. in Free Radical Biology and Medicine. 2021;177.
https://hdl.handle.net/21.15107/rcub_rimsi_1491 .

Milić Komić, Sonja, Stevanović, Strahinja, Vidović, Marija, "Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach" in Free Radical Biology and Medicine, 177 (2021),
https://hdl.handle.net/21.15107/rcub_rimsi_1491 .

TY  - CONF
AU  - Markovic, Nemanja
AU  - Milić Komić, Sonja
AU  - Radosavljevic, Jelena
AU  - Pantelic, Ana
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3084
AB  - Late embryogenesis abundant (LEA) proteins are induced in cellular dehydration, such as freezing, drought, or desiccation. They can be involved in antioxidative defense, ion sequestration, and structural stabilization of both membranes and enzymes during freezing or drying, while by forming intracellular proteinaceous condensates they increase structural integrity and intracellular viscosity of cells during desiccation 1. The genome of the model plant Arabidopsis thaliana contains 51 genes encoding LEA proteins2. The majority of these LEA proteins (35%) belongs to Pfam LEA_4 (PF02987) family. In silico analysis suggested that these proteins are highly hydrophilic proteins with significant intrinsically disordered protein (IDP) properties. In order to evaluate structural properties and possible functions of LEA_4 protein family under different water content, a representative AtLEA25 protein (At2g42560, 635 aa), naturally located in the cytoplasm of seeds3 was obtained in Escherichia coli by recombinant DNA technology. Although this technology has been traditionally used to over-express and purify various globular proteins, numerous reports have shown that the IDPs, due to their structural plasicity are naturally highly susceptible to proteolytic cleavage. To conduct structural and functional studies we developed a robust method to produce highly purified (>95% pure) AtLEA25 with no detectable amount of protein breakdown products.
PB  - Belgrade : Serbian Biochemical Society
C3  - Biochemical Insights into Molecular Mechanisms, 2021, 117-
T1  - Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology
EP  - 99
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3084
ER  - 

@conference{
author = "Markovic, Nemanja and Milić Komić, Sonja and Radosavljevic, Jelena and Pantelic, Ana and Kilibarda, Nataša and Vidović, Marija",
year = "2021",
abstract = "Late embryogenesis abundant (LEA) proteins are induced in cellular dehydration, such as freezing, drought, or desiccation. They can be involved in antioxidative defense, ion sequestration, and structural stabilization of both membranes and enzymes during freezing or drying, while by forming intracellular proteinaceous condensates they increase structural integrity and intracellular viscosity of cells during desiccation 1. The genome of the model plant Arabidopsis thaliana contains 51 genes encoding LEA proteins2. The majority of these LEA proteins (35%) belongs to Pfam LEA_4 (PF02987) family. In silico analysis suggested that these proteins are highly hydrophilic proteins with significant intrinsically disordered protein (IDP) properties. In order to evaluate structural properties and possible functions of LEA_4 protein family under different water content, a representative AtLEA25 protein (At2g42560, 635 aa), naturally located in the cytoplasm of seeds3 was obtained in Escherichia coli by recombinant DNA technology. Although this technology has been traditionally used to over-express and purify various globular proteins, numerous reports have shown that the IDPs, due to their structural plasicity are naturally highly susceptible to proteolytic cleavage. To conduct structural and functional studies we developed a robust method to produce highly purified (>95% pure) AtLEA25 with no detectable amount of protein breakdown products.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = "Biochemical Insights into Molecular Mechanisms, 2021, 117-",
title = "Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology",
pages = "99-98",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3084"
}

Markovic, N., Milić Komić, S., Radosavljevic, J., Pantelic, A., Kilibarda, N.,& Vidović, M.. (2021). Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology. in Biochemical Insights into Molecular Mechanisms, 2021, 117-
Belgrade : Serbian Biochemical Society., 98-99.
https://hdl.handle.net/21.15107/rcub_rimsi_3084

Markovic N, Milić Komić S, Radosavljevic J, Pantelic A, Kilibarda N, Vidović M. Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology. in Biochemical Insights into Molecular Mechanisms, 2021, 117-. 2021;:98-99.
https://hdl.handle.net/21.15107/rcub_rimsi_3084 .

Markovic, Nemanja, Milić Komić, Sonja, Radosavljevic, Jelena, Pantelic, Ana, Kilibarda, Nataša, Vidović, Marija, "Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology" in Biochemical Insights into Molecular Mechanisms, 2021, 117- (2021):98-99,
https://hdl.handle.net/21.15107/rcub_rimsi_3084 .

TY  - CONF
AU  - Pantelic, Ana
AU  - Stevanovic, Strahinja
AU  - Kilibarda, Nataša
AU  - Milić Komić, Sonja
AU  - Radosavljevic, Jelena
AU  - Vidović, Marija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3083
AB  - Endemic plant species, Ramonda serbica is a resurrection plant that can tolerate extreme dehydration (desiccation, loss of 95% of cellular water) even over months. The accumulation of late embryogenesis abundant proteins (LEAPs) is a crucial step in the mechanism of desiccation tolerance. The role of LEAPs is not completely resolved, but they are accepted as intrinsically disordered proteins (IDPs). Based on previously established de novo transcriptome database of R. serbica leaves we identify around 160 members of LEA gene family. Identified LEAPs were classified into six groups: LEA 1-5 and seed maturation proteins (SMPs) according to protein family (Pfam) database. Based on multiple sequence alignment, secondary structure prediction and 3D structure modeling, we conducted LEA protein structure analysis. We showed that more than 50% of identified LEAPs exhibited a high propensity to form α-helices. As predicted by several bioinformatic tools, more than 70% of identified LEAPs were found to be highly disordered. Thus, these proteins are predicted to be disordered in solution, but they acquire a secondary, predominantly α-helical structure during drying, in contrast to globular proteins, which most often causes the loss of structure upon dehydration. By using molecular dynamic simulations, we identified the most favorable conformations of representative LEAPs and we have studied conformational transitions driven by the water scarcity. Structural characterization of LEAPs is a key to understand their function and regulation of their intrinsic structural disorder-to-order transition during desiccation as a requirement for biological function, in order to promote development of new therapeutic strategies in neurodegenerative disorders, cell preservation technology and the improvement of crop drought tolerance.
PB  - Belgrade : Serbian Biochemical Society
C3  - Biochemical Insights into Molecular Mechanisms, 2021, 117-
T1  - Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves
EP  - 118
SP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3083
ER  - 

@conference{
author = "Pantelic, Ana and Stevanovic, Strahinja and Kilibarda, Nataša and Milić Komić, Sonja and Radosavljevic, Jelena and Vidović, Marija",
year = "2021",
abstract = "Endemic plant species, Ramonda serbica is a resurrection plant that can tolerate extreme dehydration (desiccation, loss of 95% of cellular water) even over months. The accumulation of late embryogenesis abundant proteins (LEAPs) is a crucial step in the mechanism of desiccation tolerance. The role of LEAPs is not completely resolved, but they are accepted as intrinsically disordered proteins (IDPs). Based on previously established de novo transcriptome database of R. serbica leaves we identify around 160 members of LEA gene family. Identified LEAPs were classified into six groups: LEA 1-5 and seed maturation proteins (SMPs) according to protein family (Pfam) database. Based on multiple sequence alignment, secondary structure prediction and 3D structure modeling, we conducted LEA protein structure analysis. We showed that more than 50% of identified LEAPs exhibited a high propensity to form α-helices. As predicted by several bioinformatic tools, more than 70% of identified LEAPs were found to be highly disordered. Thus, these proteins are predicted to be disordered in solution, but they acquire a secondary, predominantly α-helical structure during drying, in contrast to globular proteins, which most often causes the loss of structure upon dehydration. By using molecular dynamic simulations, we identified the most favorable conformations of representative LEAPs and we have studied conformational transitions driven by the water scarcity. Structural characterization of LEAPs is a key to understand their function and regulation of their intrinsic structural disorder-to-order transition during desiccation as a requirement for biological function, in order to promote development of new therapeutic strategies in neurodegenerative disorders, cell preservation technology and the improvement of crop drought tolerance.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = "Biochemical Insights into Molecular Mechanisms, 2021, 117-",
title = "Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves",
pages = "118-117",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3083"
}

Pantelic, A., Stevanovic, S., Kilibarda, N., Milić Komić, S., Radosavljevic, J.,& Vidović, M.. (2021). Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves. in Biochemical Insights into Molecular Mechanisms, 2021, 117-
Belgrade : Serbian Biochemical Society., 117-118.
https://hdl.handle.net/21.15107/rcub_rimsi_3083

Pantelic A, Stevanovic S, Kilibarda N, Milić Komić S, Radosavljevic J, Vidović M. Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves. in Biochemical Insights into Molecular Mechanisms, 2021, 117-. 2021;:117-118.
https://hdl.handle.net/21.15107/rcub_rimsi_3083 .

Pantelic, Ana, Stevanovic, Strahinja, Kilibarda, Nataša, Milić Komić, Sonja, Radosavljevic, Jelena, Vidović, Marija, "Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves" in Biochemical Insights into Molecular Mechanisms, 2021, 117- (2021):117-118,
https://hdl.handle.net/21.15107/rcub_rimsi_3083 .

TY  - CHAP
AU  - Vidović, Marija
AU  - Milić Komić, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2928
AB  - Proteolysis represents a primary recycling system for amino acids. Moreover, by controlling the protein turnover, proteolysis plays an important role in key cellular processes such as control of cell cycle, programmed cell death (including senescence) and response to various stimuli. In eukaryotic cells, most proteins are degraded by autophagy and 26S proteasome machinery, composed in general of 20S proteolytic core and 19S regulatory particle. The protein susceptibility to proteolysis is determined by its structural features. Folded globular proteins in their native state are rarely degraded, however when misfolded, denatured or when an unstructured region is attached, the degradation is promoted. Proteins natively containing intrinsically disordered regions (IDRs) or completely lacking the stable secondary and tertiary structures are defined as intrinsically disordered proteins (IDPs). Due to their high intramolecular flexibility and plasticity, IDPs are involved in DNA metabolism, transcriptional activation, autophagy, and signalling cascades related to response to various stimuli. In sessile organisms such as plants, IDPs enable prompt acclimation to external factors, including light perception, adaptation to oxidative stress and water loss, and regulation of protective, antioxidative and secondary metabolism. Binding of specific ligands and partners to particular IDP triggers structural changes and affects the stability of IDP, its susceptibility to proteolysis and aggregation-propensity. Theabnormal aggregation of several IDPs and altered proteolysis pathways are closely connected with serious neurodegenerative disorders, such as Alzheimer’s and Parkinson's diseases. In this chapter, we discuss the current understanding of proteolytic processes of specific, well-characterised IDPs under different physiological states, emphasizing the influence of the microenvironment and ligands/partners on their conformation.
T2  - A closer look at proteolysis
T1  - Regulation of proteolysis of intrinsically disordered proteins: physiological consequences
EP  - 156
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2928
ER  - 

@inbook{
author = "Vidović, Marija and Milić Komić, Sonja",
year = "2021",
abstract = "Proteolysis represents a primary recycling system for amino acids. Moreover, by controlling the protein turnover, proteolysis plays an important role in key cellular processes such as control of cell cycle, programmed cell death (including senescence) and response to various stimuli. In eukaryotic cells, most proteins are degraded by autophagy and 26S proteasome machinery, composed in general of 20S proteolytic core and 19S regulatory particle. The protein susceptibility to proteolysis is determined by its structural features. Folded globular proteins in their native state are rarely degraded, however when misfolded, denatured or when an unstructured region is attached, the degradation is promoted. Proteins natively containing intrinsically disordered regions (IDRs) or completely lacking the stable secondary and tertiary structures are defined as intrinsically disordered proteins (IDPs). Due to their high intramolecular flexibility and plasticity, IDPs are involved in DNA metabolism, transcriptional activation, autophagy, and signalling cascades related to response to various stimuli. In sessile organisms such as plants, IDPs enable prompt acclimation to external factors, including light perception, adaptation to oxidative stress and water loss, and regulation of protective, antioxidative and secondary metabolism. Binding of specific ligands and partners to particular IDP triggers structural changes and affects the stability of IDP, its susceptibility to proteolysis and aggregation-propensity. Theabnormal aggregation of several IDPs and altered proteolysis pathways are closely connected with serious neurodegenerative disorders, such as Alzheimer’s and Parkinson's diseases. In this chapter, we discuss the current understanding of proteolytic processes of specific, well-characterised IDPs under different physiological states, emphasizing the influence of the microenvironment and ligands/partners on their conformation.",
journal = "A closer look at proteolysis",
booktitle = "Regulation of proteolysis of intrinsically disordered proteins: physiological consequences",
pages = "156-111",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2928"
}

Vidović, M.,& Milić Komić, S.. (2021). Regulation of proteolysis of intrinsically disordered proteins: physiological consequences. in A closer look at proteolysis, 111-156.
https://hdl.handle.net/21.15107/rcub_rimsi_2928

Vidović M, Milić Komić S. Regulation of proteolysis of intrinsically disordered proteins: physiological consequences. in A closer look at proteolysis. 2021;:111-156.
https://hdl.handle.net/21.15107/rcub_rimsi_2928 .

Vidović, Marija, Milić Komić, Sonja, "Regulation of proteolysis of intrinsically disordered proteins: physiological consequences" in A closer look at proteolysis (2021):111-156,
https://hdl.handle.net/21.15107/rcub_rimsi_2928 .

TY  - JOUR
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Mutavdžić, Dragosav
AU  - Srećković, Tatjana
AU  - Veljović-Jovanović, Sonja
AU  - Prokic, Ljiljana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1509
AB  - Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles
IS  - 11
VL  - 10
DO  - 10.3390/plants10112308
ER  - 

@article{
author = "Živanović, Bojana and Milić Komić, Sonja and Nikolić, Nenad and Mutavdžić, Dragosav and Srećković, Tatjana and Veljović-Jovanović, Sonja and Prokic, Ljiljana",
year = "2021",
abstract = "Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles",
number = "11",
volume = "10",
doi = "10.3390/plants10112308"
}

Živanović, B., Milić Komić, S., Nikolić, N., Mutavdžić, D., Srećković, T., Veljović-Jovanović, S.,& Prokic, L.. (2021). Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles. in Plants-Basel
MDPI, Basel., 10(11).
https://doi.org/10.3390/plants10112308

Živanović B, Milić Komić S, Nikolić N, Mutavdžić D, Srećković T, Veljović-Jovanović S, Prokic L. Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles. in Plants-Basel. 2021;10(11).
doi:10.3390/plants10112308 .

Živanović, Bojana, Milić Komić, Sonja, Nikolić, Nenad, Mutavdžić, Dragosav, Srećković, Tatjana, Veljović-Jovanović, Sonja, Prokic, Ljiljana, "Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles" in Plants-Basel, 10, no. 11 (2021),
https://doi.org/10.3390/plants10112308 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Ilicic, Renata
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1438
AB  - Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers
EP  - 1959
IS  - 8
SP  - 1945
VL  - 70
DO  - 10.1111/ppa.13421
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Ilicic, Renata and Veljović-Jovanović, Sonja and Milić Komić, Sonja and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers",
pages = "1959-1945",
number = "8",
volume = "70",
doi = "10.1111/ppa.13421"
}

Marković, S., Stanković, S., Ilicic, R., Veljović-Jovanović, S., Milić Komić, S., Jelušić, A.,& Popović, T.. (2021). Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology
Wiley, Hoboken., 70(8), 1945-1959.
https://doi.org/10.1111/ppa.13421

Marković S, Stanković S, Ilicic R, Veljović-Jovanović S, Milić Komić S, Jelušić A, Popović T. Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology. 2021;70(8):1945-1959.
doi:10.1111/ppa.13421 .

Marković, Sanja, Stanković, Slaviša, Ilicic, Renata, Veljović-Jovanović, Sonja, Milić Komić, Sonja, Jelušić, Aleksandra, Popović, Tatjana, "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers" in Plant Pathology, 70, no. 8 (2021):1945-1959,
https://doi.org/10.1111/ppa.13421 . .

TY  - JOUR
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Tosti, Tomislav
AU  - Vidović, Marija
AU  - Prokic, Ljiljana
AU  - Veljović-Jovanović, Sonja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1347
AB  - Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato
IS  - 9
VL  - 9
DO  - 10.3390/plants9091147
ER  - 

@article{
author = "Živanović, Bojana and Milić Komić, Sonja and Tosti, Tomislav and Vidović, Marija and Prokic, Ljiljana and Veljović-Jovanović, Sonja",
year = "2020",
abstract = "Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato",
number = "9",
volume = "9",
doi = "10.3390/plants9091147"
}

Živanović, B., Milić Komić, S., Tosti, T., Vidović, M., Prokic, L.,& Veljović-Jovanović, S.. (2020). Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel
MDPI, Basel., 9(9).
https://doi.org/10.3390/plants9091147

Živanović B, Milić Komić S, Tosti T, Vidović M, Prokic L, Veljović-Jovanović S. Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel. 2020;9(9).
doi:10.3390/plants9091147 .

Živanović, Bojana, Milić Komić, Sonja, Tosti, Tomislav, Vidović, Marija, Prokic, Ljiljana, Veljović-Jovanović, Sonja, "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato" in Plants-Basel, 9, no. 9 (2020),
https://doi.org/10.3390/plants9091147 . .

TY  - CONF
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Milić Komić, Sonja
AU  - Veljović-Jovanović, Sonja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3090
AB  - Balkanic endemic plant species, Ramonda serbica, belongs to a small group of resurrection plants, since it is able to survive in an almost completely dehydrated state for months, and to fully recover metabolic functions upon watering. During drought and rehydration, reactive oxygen species increase presents an additional treat. In accordance, the increase of activities of the antioxidative enzymes, such as several superoxide dismutases and polyphenol oxidases in the critical first few hours of rehydration of R. serbica leaves was reported [1,2]. In fully hydrated leaves, the most abundant soluble phenolics were hydroxybenzoic acids (HBAs: p‐hydroxybenzoic, protocatechuic, and syringic acid) and catechin. Among hydroxycinnamic acids (HCAs), caffeic acid was the major one, beside ferulic and chlorogenic acids. In addition, four flavonoids: apigenin, luteolin, cyanidin and delphinidin were identified as glycosides. Although R. serbica settles exclusively north-facing steep rocky sides, in the shade of the surrounding forest canopy, with reduced solar irradiance, 5-d-exposure to supplemental UV-B radiation significantly enhanced amounts of HBA derivatives and anthocyanins (2.6 and 5.7 folds, respectively), whereas flavon glycosides and most of HCA derivatives were unaffected. The physiological aspect of this UV-B stimulative effect, as well as the role of phenolic metabolism in cellular desiccation survival has to be explained.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137
T1  - Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc.
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3090
ER  - 

@conference{
author = "Vidović, Marija and Morina, Filis and Milić Komić, Sonja and Veljović-Jovanović, Sonja",
year = "2019",
abstract = "Balkanic endemic plant species, Ramonda serbica, belongs to a small group of resurrection plants, since it is able to survive in an almost completely dehydrated state for months, and to fully recover metabolic functions upon watering. During drought and rehydration, reactive oxygen species increase presents an additional treat. In accordance, the increase of activities of the antioxidative enzymes, such as several superoxide dismutases and polyphenol oxidases in the critical first few hours of rehydration of R. serbica leaves was reported [1,2]. In fully hydrated leaves, the most abundant soluble phenolics were hydroxybenzoic acids (HBAs: p‐hydroxybenzoic, protocatechuic, and syringic acid) and catechin. Among hydroxycinnamic acids (HCAs), caffeic acid was the major one, beside ferulic and chlorogenic acids. In addition, four flavonoids: apigenin, luteolin, cyanidin and delphinidin were identified as glycosides. Although R. serbica settles exclusively north-facing steep rocky sides, in the shade of the surrounding forest canopy, with reduced solar irradiance, 5-d-exposure to supplemental UV-B radiation significantly enhanced amounts of HBA derivatives and anthocyanins (2.6 and 5.7 folds, respectively), whereas flavon glycosides and most of HCA derivatives were unaffected. The physiological aspect of this UV-B stimulative effect, as well as the role of phenolic metabolism in cellular desiccation survival has to be explained.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137",
title = "Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc.",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3090"
}

Vidović, M., Morina, F., Milić Komić, S.,& Veljović-Jovanović, S.. (2019). Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc.. in 13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 137.
https://hdl.handle.net/21.15107/rcub_rimsi_3090

Vidović M, Morina F, Milić Komić S, Veljović-Jovanović S. Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc.. in 13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137. 2019;:137.
https://hdl.handle.net/21.15107/rcub_rimsi_3090 .

Vidović, Marija, Morina, Filis, Milić Komić, Sonja, Veljović-Jovanović, Sonja, "Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc." in 13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137 (2019):137,
https://hdl.handle.net/21.15107/rcub_rimsi_3090 .

TY  - THES
AU  - Milić Komić, Sonja
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3191
AB  - U ovoj tezi ispitivane su antioksidativne aktivnosti (AA) slobodnih aminokiselina (glicin,
alanin, prolin, valin, izoleucin, leucin, fenilalanin, triptofan, tirozin, serin, treonin, asparagin,
glutamin, histidin, asparaginska kiselina, glutaminska kiselina, lizin, arginin, cistein i
metionin, homocistein, hidroksiprolin, norleucin, 2-aminobutanska kiselina i
3,4-dihidroksifenilalanin) u Fentonovom sistemu. Redosled reaktivnosti aminokiselina u
reakciji sa hidroksil-radikalom, antioksidativne aktivnosti (HO•
) bio je sledeći: Trp >
norleucin > Phe, Leu > Ile > His >3,4-dihidroksifenilalanin, Arg > Val > Lys, Tyr, Pro >
hidroksiprolin > α-aminobutanska kiselina > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp.
Aminokiseline koje sadrže sumpor su u reakciji sa hidroksil-radikalom davale više različitih
radikalskih vrsta koje su ispitivane EPR metodom sa spinskim hvatačima.
Korelaciona analiza dobijenih eksperimentalnih podataka sa literaturno poznatim
parametrima koji karakterišu različite osobine slobodnih aminokiselina je pokazala pozitivnu
korelaciju između AA i hidrofobnosti. Kada se podele aminokiseline (AK), kod
nenaelektrisanih aminokiselina je uočena vrlo jaka korelacija sa hidrofobnošću. Kod
naelektrisanih AK se ne primećuje korelacija, ili je korelacija negativna, zavisno od
primenjene skale parametara. Jaka negativna korelacija je nađena kod AA i polarnosti. Sterni
parametri i hidratacioni brojevi su pokazali pozitivnu korelaciju AA sa AK sa nepolarnim
bočnim lancem. Smanjenje temperature koje podstiče hidrofobnu hidrataciju je uzrokovalo
povećane vrednosti AA, što ukazuje na to da je oksidacija AK sa HO•
zavisna od hidrofobne
hidratacije. Ova istraživanja se mogu primeniti u procesima prečišćavanja prirodnih i
otpadnih voda, s obzirom na to da AK ulaze u sastav prirodnog organskog materijala.
Aminokiselina sa najvećom vrednošću za antioksidativnu aktivnost je bila triptofan u reakciji
sa HO• u Fentonovom sistemu. U cilju boljeg razumevanja reaktivnosti triptofana i njegovih
derivata, odabran je indol kao model-jedinjenje za dalje ispitivanje interakcija aromatičnih
aminokiselina. Interakcije gvožđa i organskih supstanci su prisutne u mnogim aspektima
iii
zaštite životne sredine, uključujući foto-redoks reakcije, recikliranje i dostupnost gvožđa. U
ovoj tezi ispitivane su redoks reakcije gvožđa i indola u vodi u prisustvu UV-zračenja.
Prisustvo jednog molekula indola dovelo je do prisustva osam jona redukovanog gvožđa, bez
doprinosa direktne redukcije Fe3+ pod uticajem svetlosti, koja daje Fe2+ i HO• kao proizvode
reakcije. Glavni mehanizmi koji dovode do redukcije Fe(III) u prisustvu indola, tj.
akumulacije Fe(II) su: hvatanje slobodnog radikala HO•
, što sprečava oksidaciju Fe(II) sa
HO•
; oksidacija indola i njegovih derivata sa ekscitovanim gvožđem; redukcija gvožđa sa
ekscitovanim indolom (kod UV-A zračenja nije prisutno). Hidratisani elektroni nemaju
veliku ulogu u redukciji Fe. Radikali indola su primećeni kao nusproizvodi u interakcijama
indola i gvožđa.
Kompleks niske simetrije indola i Fe3+ je potvrđen primenom EPR metode. Formiranje
kompleksa potpomaže transfer elektrona sa ekscitovanog indola na Fe3+.
Ova istraživanja su važna za razumevanje procesa foto-redukcije gvožđa sa heterocikličnim
aromatičnim zagađivačima i unapređenje protokola za prečišćavanje indola u otpadnim
vodama.
AB  - In this thesis antioxidative activities (AA) of free L-amino acids (glycine, alanine, proline,
valine, isoleucine, leucine, phenylalanine, tryptophane, tyrosine, serine, threonine,
asparagine, glutamine, histidine, aspartic acid, glutamic acid, lysine, arginine, cystein and
methionine, homocysteine, hydroxyproline, norleucine, α-aminobutyric acid and
3,4-dihydroxyphenylalanine) against Fenton system-mediated hydroxyl radical (HO•
)
production in aqueous solution were studied. The rank order according to AA was: Trp >
norleucine > Phe, Leu > Ile > His >3,4-dihydroxyphenylalanine, Arg > Val > Lys, Tyr, Pro >
hydroxyproline> α-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp. Sulfurcontaining FAA generated different secondary reactive products, which were discriminated
by the means of electron paramagnetic resonance spin-trapping spectroscopy.
Correlation analysis between experimental data and a set of physicochemical parameters
known from available literature showed positive correlation with hydrophobicity. When
taken separately, uncharged FAA exhibited strong positive correlation of AA with
hydrophobicity whereas charged FAA showed negative or no significant correlation
depending on the scale applied. A general strong negative correlation was found between AA
and polarity. Steric parameters and hydration numbers correlated positively with AA of
nonpolar side-chain FAA. Decrease of temperature which promotes hydrophobic hydration
resulted in increased AA, which implies that HO•
-provoked oxidation of FAA is strongly
affected by hydrophobic hydration. Our findings are important for the understanding of
oxidation processes in natural and waste waters, considering the amount of amino acids in
natural organic material.
Amino acid with the highest value for AA in the reaction with HO• in Fenton system was
tryptophan. With the goal of better understanding of the reactivity of tryptophan and it’s
derivatives indole was chosen as a model compound for further examination of interactions
of aromatic amino acids. Iron–organic substances interactions are involved in a variety of
environmental phenomena, including photo-redox reactions, iron cycling and bioavailability,
v
as well as contaminant fate. In this thesis UV-induced redox reactions of iron and indole in
water were examined. The presence of one indole molecule in the irradiated system resulted
in the presence of eight reduced ferric ions, not counting direct reduction of Fe3+ in the
presence of light, which gives Fe2+ and hydroxyl radical (HO•
) as products. The main
mechanisms that contribute to indole-related Fe3+ reduction i.e. Fe2+ accumulation are: (i)
HO• scavenging, which prevents oxidation of Fe2+ by HO•
; (ii) oxidation of indole and its
derivatives by excited ferric iron; (iii) reduction of ferric iron by excited indole (not present
under UV-A). Hydrated electrons released by UV-B-excited indole play only a minor role in
the reduction of iron. Indole-derived radicals emerged as byproducts of indole/iron photochemistry.
Low-T EPR spectroscopy showed that indole forms a weak low-symmetry complex with
Fe3+. The formation of complex promotes electron transfer from excited indole to Fe3+. Our
findings are important for understanding the catalysis of photo-reduction of iron by
heterocyclic aromatic pollutants, and for the development of protocols for indole processing
in wastewaters.
T2  - Универзитет у Београду, Хемијски факултет
T1  - REDOKS SVOJSTVA SLOBODNIH AMINOKISELINA I INDOLA KAO MODELJEDINJENJA U FENTONOVOM SISTEMU
T1  - REDOX PROPERTIES OF FREE AMINO ACIDS AND INDOLE AS A MODEL COMPOUND IN THE FENTON SYSTEM
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3191
ER  - 

@phdthesis{
author = "Milić Komić, Sonja",
year = "2018",
abstract = "U ovoj tezi ispitivane su antioksidativne aktivnosti (AA) slobodnih aminokiselina (glicin,
alanin, prolin, valin, izoleucin, leucin, fenilalanin, triptofan, tirozin, serin, treonin, asparagin,
glutamin, histidin, asparaginska kiselina, glutaminska kiselina, lizin, arginin, cistein i
metionin, homocistein, hidroksiprolin, norleucin, 2-aminobutanska kiselina i
3,4-dihidroksifenilalanin) u Fentonovom sistemu. Redosled reaktivnosti aminokiselina u
reakciji sa hidroksil-radikalom, antioksidativne aktivnosti (HO•
) bio je sledeći: Trp >
norleucin > Phe, Leu > Ile > His >3,4-dihidroksifenilalanin, Arg > Val > Lys, Tyr, Pro >
hidroksiprolin > α-aminobutanska kiselina > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp.
Aminokiseline koje sadrže sumpor su u reakciji sa hidroksil-radikalom davale više različitih
radikalskih vrsta koje su ispitivane EPR metodom sa spinskim hvatačima.
Korelaciona analiza dobijenih eksperimentalnih podataka sa literaturno poznatim
parametrima koji karakterišu različite osobine slobodnih aminokiselina je pokazala pozitivnu
korelaciju između AA i hidrofobnosti. Kada se podele aminokiseline (AK), kod
nenaelektrisanih aminokiselina je uočena vrlo jaka korelacija sa hidrofobnošću. Kod
naelektrisanih AK se ne primećuje korelacija, ili je korelacija negativna, zavisno od
primenjene skale parametara. Jaka negativna korelacija je nađena kod AA i polarnosti. Sterni
parametri i hidratacioni brojevi su pokazali pozitivnu korelaciju AA sa AK sa nepolarnim
bočnim lancem. Smanjenje temperature koje podstiče hidrofobnu hidrataciju je uzrokovalo
povećane vrednosti AA, što ukazuje na to da je oksidacija AK sa HO•
zavisna od hidrofobne
hidratacije. Ova istraživanja se mogu primeniti u procesima prečišćavanja prirodnih i
otpadnih voda, s obzirom na to da AK ulaze u sastav prirodnog organskog materijala.
Aminokiselina sa najvećom vrednošću za antioksidativnu aktivnost je bila triptofan u reakciji
sa HO• u Fentonovom sistemu. U cilju boljeg razumevanja reaktivnosti triptofana i njegovih
derivata, odabran je indol kao model-jedinjenje za dalje ispitivanje interakcija aromatičnih
aminokiselina. Interakcije gvožđa i organskih supstanci su prisutne u mnogim aspektima
iii
zaštite životne sredine, uključujući foto-redoks reakcije, recikliranje i dostupnost gvožđa. U
ovoj tezi ispitivane su redoks reakcije gvožđa i indola u vodi u prisustvu UV-zračenja.
Prisustvo jednog molekula indola dovelo je do prisustva osam jona redukovanog gvožđa, bez
doprinosa direktne redukcije Fe3+ pod uticajem svetlosti, koja daje Fe2+ i HO• kao proizvode
reakcije. Glavni mehanizmi koji dovode do redukcije Fe(III) u prisustvu indola, tj.
akumulacije Fe(II) su: hvatanje slobodnog radikala HO•
, što sprečava oksidaciju Fe(II) sa
HO•
; oksidacija indola i njegovih derivata sa ekscitovanim gvožđem; redukcija gvožđa sa
ekscitovanim indolom (kod UV-A zračenja nije prisutno). Hidratisani elektroni nemaju
veliku ulogu u redukciji Fe. Radikali indola su primećeni kao nusproizvodi u interakcijama
indola i gvožđa.
Kompleks niske simetrije indola i Fe3+ je potvrđen primenom EPR metode. Formiranje
kompleksa potpomaže transfer elektrona sa ekscitovanog indola na Fe3+.
Ova istraživanja su važna za razumevanje procesa foto-redukcije gvožđa sa heterocikličnim
aromatičnim zagađivačima i unapređenje protokola za prečišćavanje indola u otpadnim
vodama., In this thesis antioxidative activities (AA) of free L-amino acids (glycine, alanine, proline,
valine, isoleucine, leucine, phenylalanine, tryptophane, tyrosine, serine, threonine,
asparagine, glutamine, histidine, aspartic acid, glutamic acid, lysine, arginine, cystein and
methionine, homocysteine, hydroxyproline, norleucine, α-aminobutyric acid and
3,4-dihydroxyphenylalanine) against Fenton system-mediated hydroxyl radical (HO•
)
production in aqueous solution were studied. The rank order according to AA was: Trp >
norleucine > Phe, Leu > Ile > His >3,4-dihydroxyphenylalanine, Arg > Val > Lys, Tyr, Pro >
hydroxyproline> α-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp. Sulfurcontaining FAA generated different secondary reactive products, which were discriminated
by the means of electron paramagnetic resonance spin-trapping spectroscopy.
Correlation analysis between experimental data and a set of physicochemical parameters
known from available literature showed positive correlation with hydrophobicity. When
taken separately, uncharged FAA exhibited strong positive correlation of AA with
hydrophobicity whereas charged FAA showed negative or no significant correlation
depending on the scale applied. A general strong negative correlation was found between AA
and polarity. Steric parameters and hydration numbers correlated positively with AA of
nonpolar side-chain FAA. Decrease of temperature which promotes hydrophobic hydration
resulted in increased AA, which implies that HO•
-provoked oxidation of FAA is strongly
affected by hydrophobic hydration. Our findings are important for the understanding of
oxidation processes in natural and waste waters, considering the amount of amino acids in
natural organic material.
Amino acid with the highest value for AA in the reaction with HO• in Fenton system was
tryptophan. With the goal of better understanding of the reactivity of tryptophan and it’s
derivatives indole was chosen as a model compound for further examination of interactions
of aromatic amino acids. Iron–organic substances interactions are involved in a variety of
environmental phenomena, including photo-redox reactions, iron cycling and bioavailability,
v
as well as contaminant fate. In this thesis UV-induced redox reactions of iron and indole in
water were examined. The presence of one indole molecule in the irradiated system resulted
in the presence of eight reduced ferric ions, not counting direct reduction of Fe3+ in the
presence of light, which gives Fe2+ and hydroxyl radical (HO•
) as products. The main
mechanisms that contribute to indole-related Fe3+ reduction i.e. Fe2+ accumulation are: (i)
HO• scavenging, which prevents oxidation of Fe2+ by HO•
; (ii) oxidation of indole and its
derivatives by excited ferric iron; (iii) reduction of ferric iron by excited indole (not present
under UV-A). Hydrated electrons released by UV-B-excited indole play only a minor role in
the reduction of iron. Indole-derived radicals emerged as byproducts of indole/iron photochemistry.
Low-T EPR spectroscopy showed that indole forms a weak low-symmetry complex with
Fe3+. The formation of complex promotes electron transfer from excited indole to Fe3+. Our
findings are important for understanding the catalysis of photo-reduction of iron by
heterocyclic aromatic pollutants, and for the development of protocols for indole processing
in wastewaters.",
journal = "Универзитет у Београду, Хемијски факултет",
title = "REDOKS SVOJSTVA SLOBODNIH AMINOKISELINA I INDOLA KAO MODELJEDINJENJA U FENTONOVOM SISTEMU, REDOX PROPERTIES OF FREE AMINO ACIDS AND INDOLE AS A MODEL COMPOUND IN THE FENTON SYSTEM",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3191"
}

Milić Komić, S.. (2018). REDOKS SVOJSTVA SLOBODNIH AMINOKISELINA I INDOLA KAO MODELJEDINJENJA U FENTONOVOM SISTEMU. in Универзитет у Београду, Хемијски факултет.
https://hdl.handle.net/21.15107/rcub_rimsi_3191

Milić Komić S. REDOKS SVOJSTVA SLOBODNIH AMINOKISELINA I INDOLA KAO MODELJEDINJENJA U FENTONOVOM SISTEMU. in Универзитет у Београду, Хемијски факултет. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_3191 .

Milić Komić, Sonja, "REDOKS SVOJSTVA SLOBODNIH AMINOKISELINA I INDOLA KAO MODELJEDINJENJA U FENTONOVOM SISTEMU" in Универзитет у Београду, Хемијски факултет (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_3191 .

TY  - JOUR
AU  - Živanović, Bojana
AU  - Vidović, Marija
AU  - Milić Komić, Sonja
AU  - Jovanović, Ljubinko
AU  - Kolarz, Predrag
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1066
AB  - Tomato (Solanum lycopersicum L.) is among the economically most important vegetables in Europe, valued for its bioactive properties due to significant contents of vitamins, carotenoids, and phenolic compounds. In this study, the tomato cultivar Big Beef F1 was grown in the open field (OF) and under polytunnels in central Serbia during 3 years. Polytunnels were covered with two foils (both with 57% reduced photosynthetic active radiation, PAR) differing in UV-A and UV-B transmittance. The aim of our work was to determine the influence of light conditions on accumulation of phytonutrients (carotenoids and phenolics) in the peel and flesh of ripe tomato fruits. The amount of effective antioxidants, caffeic acid, and quercetin (phenolics with ortho-dihydroxy substitution) in the peel was the highest in tomato fruits grown in the OF (maximal PAR and UV-A and UV-B radiation). Moreover, the content of leaf epidermal flavonoids was the highest in the OF. The content of lycopene and beta-carotene in the flesh of tomato fruit was higher under the polytunnel with higher UV-transmittance. Our results showed that selection of the right light conditions (quality and intensity) for tomato production has a significant effect on the accumulation of beneficial phenolics and carotenoids.
PB  - Tubitak Scientific & Technical Research Council Turkey, Ankara
T2  - Turkish Journal of Agriculture and Forestry
T1  - Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates
EP  - +
IS  - 2
SP  - 113
VL  - 41
DO  - 10.3906/tar-1612-56
ER  - 

@article{
author = "Živanović, Bojana and Vidović, Marija and Milić Komić, Sonja and Jovanović, Ljubinko and Kolarz, Predrag and Morina, Filis and Veljović-Jovanović, Sonja",
year = "2017",
abstract = "Tomato (Solanum lycopersicum L.) is among the economically most important vegetables in Europe, valued for its bioactive properties due to significant contents of vitamins, carotenoids, and phenolic compounds. In this study, the tomato cultivar Big Beef F1 was grown in the open field (OF) and under polytunnels in central Serbia during 3 years. Polytunnels were covered with two foils (both with 57% reduced photosynthetic active radiation, PAR) differing in UV-A and UV-B transmittance. The aim of our work was to determine the influence of light conditions on accumulation of phytonutrients (carotenoids and phenolics) in the peel and flesh of ripe tomato fruits. The amount of effective antioxidants, caffeic acid, and quercetin (phenolics with ortho-dihydroxy substitution) in the peel was the highest in tomato fruits grown in the OF (maximal PAR and UV-A and UV-B radiation). Moreover, the content of leaf epidermal flavonoids was the highest in the OF. The content of lycopene and beta-carotene in the flesh of tomato fruit was higher under the polytunnel with higher UV-transmittance. Our results showed that selection of the right light conditions (quality and intensity) for tomato production has a significant effect on the accumulation of beneficial phenolics and carotenoids.",
publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara",
journal = "Turkish Journal of Agriculture and Forestry",
title = "Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates",
pages = "+-113",
number = "2",
volume = "41",
doi = "10.3906/tar-1612-56"
}

Živanović, B., Vidović, M., Milić Komić, S., Jovanović, L., Kolarz, P., Morina, F.,& Veljović-Jovanović, S.. (2017). Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates. in Turkish Journal of Agriculture and Forestry
Tubitak Scientific & Technical Research Council Turkey, Ankara., 41(2), 113-+.
https://doi.org/10.3906/tar-1612-56

Živanović B, Vidović M, Milić Komić S, Jovanović L, Kolarz P, Morina F, Veljović-Jovanović S. Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates. in Turkish Journal of Agriculture and Forestry. 2017;41(2):113-+.
doi:10.3906/tar-1612-56 .

Živanović, Bojana, Vidović, Marija, Milić Komić, Sonja, Jovanović, Ljubinko, Kolarz, Predrag, Morina, Filis, Veljović-Jovanović, Sonja, "Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates" in Turkish Journal of Agriculture and Forestry, 41, no. 2 (2017):113-+,
https://doi.org/10.3906/tar-1612-56 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Prokic, Ljiljana
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/999
AB  - In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat
EP  - 39
SP  - 25
VL  - 206
DO  - 10.1016/j.jplph.2016.07.017
ER  - 

@article{
author = "Vidović, Marija and Morina, Filis and Prokic, Ljiljana and Milić Komić, Sonja and Živanović, Bojana and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat",
pages = "39-25",
volume = "206",
doi = "10.1016/j.jplph.2016.07.017"
}

Vidović, M., Morina, F., Prokic, L., Milić Komić, S., Živanović, B.,& Veljović-Jovanović, S.. (2016). Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 206, 25-39.
https://doi.org/10.1016/j.jplph.2016.07.017

Vidović M, Morina F, Prokic L, Milić Komić S, Živanović B, Veljović-Jovanović S. Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology. 2016;206:25-39.
doi:10.1016/j.jplph.2016.07.017 .

Vidović, Marija, Morina, Filis, Prokic, Ljiljana, Milić Komić, Sonja, Živanović, Bojana, Veljović-Jovanović, Sonja, "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat" in Journal of Plant Physiology, 206 (2016):25-39,
https://doi.org/10.1016/j.jplph.2016.07.017 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Milić Komić, Sonja
AU  - Vuleta, A.
AU  - Zechmann, Bernd
AU  - Prokic, Ljiljana
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/959
AB  - Hydrogen peroxide is an important signalling molecule, involved in regulation of numerous metabolic processes in plants. The most important sources of H2O2 in photosynthetically active cells are chloroplasts and peroxisomes. Here we employed variegated Pelargonium zonale to characterise and compare enzymatic and non-enzymatic components of the antioxidative system in autotrophic and heterotrophic leaf tissues at (sub)cellular level under optimal growth conditions. The results revealed that both leaf tissues had specific strategies to regulate H2O2 levels. In photosynthetic cells, the redox regulatory system was based on ascorbate, and on the activities of thylakoid-bound ascorbate peroxidase (tAPX) and catalase. In this leaf tissue, ascorbate was predominantly localised in the nucleus, peroxisomes, plastids and mitochondria. On the other hand, non-photosynthetic cells contained higher glutathione content, mostly located in mitochondria. The enzymatic antioxidative system in non-photosynthetic cells relied on the ascorbate-glutathione cycle and both Mn and Cu/Zn superoxide dismutase. Interestingly, higher content of ascorbate and glutathione, and higher activities of APX in the cytosol of non-photosynthetic leaf cells compared to the photosynthetic ones, suggest the importance of this compartment in H2O2 regulation. Together, these results imply different regulation of processes linked with H2O2 signalling at subcellular level. Thus, we propose green-white variegated leaves as an excellent system for examination of redox signal transduction and redox communication between two cell types, autotrophic and heterotrophic, within the same organ.
PB  - Wiley, Hoboken
T2  - Plant Biology
T1  - Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants
EP  - 680
IS  - 4
SP  - 669
VL  - 18
DO  - 10.1111/plb.12429
ER  - 

@article{
author = "Vidović, Marija and Morina, Filis and Milić Komić, Sonja and Vuleta, A. and Zechmann, Bernd and Prokic, Ljiljana and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "Hydrogen peroxide is an important signalling molecule, involved in regulation of numerous metabolic processes in plants. The most important sources of H2O2 in photosynthetically active cells are chloroplasts and peroxisomes. Here we employed variegated Pelargonium zonale to characterise and compare enzymatic and non-enzymatic components of the antioxidative system in autotrophic and heterotrophic leaf tissues at (sub)cellular level under optimal growth conditions. The results revealed that both leaf tissues had specific strategies to regulate H2O2 levels. In photosynthetic cells, the redox regulatory system was based on ascorbate, and on the activities of thylakoid-bound ascorbate peroxidase (tAPX) and catalase. In this leaf tissue, ascorbate was predominantly localised in the nucleus, peroxisomes, plastids and mitochondria. On the other hand, non-photosynthetic cells contained higher glutathione content, mostly located in mitochondria. The enzymatic antioxidative system in non-photosynthetic cells relied on the ascorbate-glutathione cycle and both Mn and Cu/Zn superoxide dismutase. Interestingly, higher content of ascorbate and glutathione, and higher activities of APX in the cytosol of non-photosynthetic leaf cells compared to the photosynthetic ones, suggest the importance of this compartment in H2O2 regulation. Together, these results imply different regulation of processes linked with H2O2 signalling at subcellular level. Thus, we propose green-white variegated leaves as an excellent system for examination of redox signal transduction and redox communication between two cell types, autotrophic and heterotrophic, within the same organ.",
publisher = "Wiley, Hoboken",
journal = "Plant Biology",
title = "Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants",
pages = "680-669",
number = "4",
volume = "18",
doi = "10.1111/plb.12429"
}

Vidović, M., Morina, F., Milić Komić, S., Vuleta, A., Zechmann, B., Prokic, L.,& Veljović-Jovanović, S.. (2016). Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants. in Plant Biology
Wiley, Hoboken., 18(4), 669-680.
https://doi.org/10.1111/plb.12429

Vidović M, Morina F, Milić Komić S, Vuleta A, Zechmann B, Prokic L, Veljović-Jovanović S. Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants. in Plant Biology. 2016;18(4):669-680.
doi:10.1111/plb.12429 .

Vidović, Marija, Morina, Filis, Milić Komić, Sonja, Vuleta, A., Zechmann, Bernd, Prokic, Ljiljana, Veljović-Jovanović, Sonja, "Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants" in Plant Biology, 18, no. 4 (2016):669-680,
https://doi.org/10.1111/plb.12429 . .