Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat
Само за регистроване кориснике
2016
Аутори
Vidović, MarijaMorina, Filis
Prokic, Ljiljana
Milić Komić, Sonja
Živanović, Bojana
Veljović-Jovanović, Sonja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat additio...n stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.
Кључне речи:
Variegated plants / Strong light / Paraquat / Hydrogen peroxide / Ascorbate-glutathione cycle / Abscisic acidИзвор:
Journal of Plant Physiology, 2016, 206, 25-39Издавач:
- Elsevier Gmbh, Munich
Финансирање / пројекти:
- Модификације антиоксидативног метаболизма биљака са циљем повећања толеранције на абиотски стрес и идентификација нових биомаркера са применом у ремедијацији и мониторингу деградираних станишта (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
DOI: 10.1016/j.jplph.2016.07.017
ISSN: 0176-1617
PubMed: 27688091
WoS: 000389596500003
Scopus: 2-s2.0-84988719255
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Vidović, Marija AU - Morina, Filis AU - Prokic, Ljiljana AU - Milić Komić, Sonja AU - Živanović, Bojana AU - Veljović-Jovanović, Sonja PY - 2016 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/999 AB - In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed. PB - Elsevier Gmbh, Munich T2 - Journal of Plant Physiology T1 - Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat EP - 39 SP - 25 VL - 206 DO - 10.1016/j.jplph.2016.07.017 ER -
@article{ author = "Vidović, Marija and Morina, Filis and Prokic, Ljiljana and Milić Komić, Sonja and Živanović, Bojana and Veljović-Jovanović, Sonja", year = "2016", abstract = "In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.", publisher = "Elsevier Gmbh, Munich", journal = "Journal of Plant Physiology", title = "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat", pages = "39-25", volume = "206", doi = "10.1016/j.jplph.2016.07.017" }
Vidović, M., Morina, F., Prokic, L., Milić Komić, S., Živanović, B.,& Veljović-Jovanović, S.. (2016). Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology Elsevier Gmbh, Munich., 206, 25-39. https://doi.org/10.1016/j.jplph.2016.07.017
Vidović M, Morina F, Prokic L, Milić Komić S, Živanović B, Veljović-Jovanović S. Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology. 2016;206:25-39. doi:10.1016/j.jplph.2016.07.017 .
Vidović, Marija, Morina, Filis, Prokic, Ljiljana, Milić Komić, Sonja, Živanović, Bojana, Veljović-Jovanović, Sonja, "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat" in Journal of Plant Physiology, 206 (2016):25-39, https://doi.org/10.1016/j.jplph.2016.07.017 . .