Živanović, Bojana

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  • Živanović, Bojana (16)

Author's Bibliography

Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season

Živanović, Bojana; Milić Komić, Sonja; Sedlarević Zorić, Ana; Jelušić, Aleksandra; Šušić, Nikola; Marković, Sanja; Veljović-Jovanović, Sonja

(University of Belgrade, Technical Faculty in Bor, 2023)

TY  - CONF
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Sedlarević Zorić, Ana
AU  - Jelušić, Aleksandra
AU  - Šušić, Nikola
AU  - Marković, Sanja
AU  - Veljović-Jovanović, Sonja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2058
AB  - Due to increased urbanization and industrialization, the emission of toxic material into the
atmosphere is in expansion, which has a negative impact on the environment and human health. In
this research, we monitored the effect of air pollution on the peroxidase (POD) activity and total
antioxidant capacity of different tree species during the growing season. The main goal was to
determine which tree species developed the highest tolerance to unfavorable environmental
conditions at the end of growing season, based on the response of their antioxidative metabolism. The
greatest change in POD activity was observed in the Fagus sylvatica L. leaves, where enzyme activity
was more than doubled in the autumn, in comparison to spring. On the other hand, decrease in POD
activity was the greatest in Cedrus atlantica (Endl.) Manetti ex Carrière needles. Moreover, total
antioxidative capacity was altered during growing season in almost all examined tree species. The
Magnolia spp. showed the most consistent response to the given environmental pollution with both
portrayed parameters induced during growing season. In general, we can conclude that the tree
species investigated in this research possess distinctive tolerance potential to air pollutants.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th International Conference Ecological Truth & Environmental Research
T1  - Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season
EP  - 134
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2058
ER  - 
@conference{
author = "Živanović, Bojana and Milić Komić, Sonja and Sedlarević Zorić, Ana and Jelušić, Aleksandra and Šušić, Nikola and Marković, Sanja and Veljović-Jovanović, Sonja",
year = "2023",
abstract = "Due to increased urbanization and industrialization, the emission of toxic material into the
atmosphere is in expansion, which has a negative impact on the environment and human health. In
this research, we monitored the effect of air pollution on the peroxidase (POD) activity and total
antioxidant capacity of different tree species during the growing season. The main goal was to
determine which tree species developed the highest tolerance to unfavorable environmental
conditions at the end of growing season, based on the response of their antioxidative metabolism. The
greatest change in POD activity was observed in the Fagus sylvatica L. leaves, where enzyme activity
was more than doubled in the autumn, in comparison to spring. On the other hand, decrease in POD
activity was the greatest in Cedrus atlantica (Endl.) Manetti ex Carrière needles. Moreover, total
antioxidative capacity was altered during growing season in almost all examined tree species. The
Magnolia spp. showed the most consistent response to the given environmental pollution with both
portrayed parameters induced during growing season. In general, we can conclude that the tree
species investigated in this research possess distinctive tolerance potential to air pollutants.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th International Conference Ecological Truth & Environmental Research",
title = "Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season",
pages = "134-129",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2058"
}
Živanović, B., Milić Komić, S., Sedlarević Zorić, A., Jelušić, A., Šušić, N., Marković, S.,& Veljović-Jovanović, S.. (2023). Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season. in 30th International Conference Ecological Truth & Environmental Research
University of Belgrade, Technical Faculty in Bor., 129-134.
https://hdl.handle.net/21.15107/rcub_rimsi_2058
Živanović B, Milić Komić S, Sedlarević Zorić A, Jelušić A, Šušić N, Marković S, Veljović-Jovanović S. Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season. in 30th International Conference Ecological Truth & Environmental Research. 2023;:129-134.
https://hdl.handle.net/21.15107/rcub_rimsi_2058 .
Živanović, Bojana, Milić Komić, Sonja, Sedlarević Zorić, Ana, Jelušić, Aleksandra, Šušić, Nikola, Marković, Sanja, Veljović-Jovanović, Sonja, "Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season" in 30th International Conference Ecological Truth & Environmental Research (2023):129-134,
https://hdl.handle.net/21.15107/rcub_rimsi_2058 .

ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION

Šušić, Nikola; Milić Komić, Sonja; Živanović, Bojana; Jelušić, Aleksandra; Marković, Sanja; Sedlarević Zorić, Ana; Veljović-Jovanović, Sonja

(University of Belgrade, Technical Faculty in Bor, 2023)

TY  - CONF
AU  - Šušić, Nikola
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Sedlarević Zorić, Ana
AU  - Veljović-Jovanović, Sonja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1984
AB  - The first months are the most sensitive phase of oak seedling development. Light conditions have an important role in this sense, both from the physiological and management viewpoint. We investigated the response of pedunculate oak seedlings to three growth light intensities (100, 550 and 2000 μmol cm−2s−1) during development of the first and second growth flush. The low and high light intensities of PAR are supposed to mimic the effects of solar radiation under extreme natural conditions (closed canopy and open field). The response of seedlings to different light intensities was evaluated by determining the photochemical activity of photosystem II, leaf chlorophyll concentration and epidermal flavonoid accumulation for both growth flushes. At the end of the experiment (after 4.5 months) the effects of different treatments on growth parameters were also determined. We showed here that oak seedlings responded to varying light intensities by modifying their physiological and morphological traits of successive growth flushes. At medium light, seedlings had the highest PSII photochemical activity in the 2nd flush. High light induced very low photochemical efficiency of photosystem II in both growth flushes indicating the development of high non-photochemical quenching of chlorophyll fluorescence as part of photoprotective mechanism. In accordance with high photosynthetic yield at ML, the investment of photosynthates in growth, especially biomass allocation towards root system was confirmed. ML was optimal for seedling development in the first months. The results may contribute to a better understanding of oak seedling development and acclimation and could have importance for oak natural regeneration.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
T1  - ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION
EP  - 140
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1984
ER  - 
@conference{
author = "Šušić, Nikola and Milić Komić, Sonja and Živanović, Bojana and Jelušić, Aleksandra and Marković, Sanja and Sedlarević Zorić, Ana and Veljović-Jovanović, Sonja",
year = "2023",
abstract = "The first months are the most sensitive phase of oak seedling development. Light conditions have an important role in this sense, both from the physiological and management viewpoint. We investigated the response of pedunculate oak seedlings to three growth light intensities (100, 550 and 2000 μmol cm−2s−1) during development of the first and second growth flush. The low and high light intensities of PAR are supposed to mimic the effects of solar radiation under extreme natural conditions (closed canopy and open field). The response of seedlings to different light intensities was evaluated by determining the photochemical activity of photosystem II, leaf chlorophyll concentration and epidermal flavonoid accumulation for both growth flushes. At the end of the experiment (after 4.5 months) the effects of different treatments on growth parameters were also determined. We showed here that oak seedlings responded to varying light intensities by modifying their physiological and morphological traits of successive growth flushes. At medium light, seedlings had the highest PSII photochemical activity in the 2nd flush. High light induced very low photochemical efficiency of photosystem II in both growth flushes indicating the development of high non-photochemical quenching of chlorophyll fluorescence as part of photoprotective mechanism. In accordance with high photosynthetic yield at ML, the investment of photosynthates in growth, especially biomass allocation towards root system was confirmed. ML was optimal for seedling development in the first months. The results may contribute to a better understanding of oak seedling development and acclimation and could have importance for oak natural regeneration.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23",
title = "ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION",
pages = "140-135",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1984"
}
Šušić, N., Milić Komić, S., Živanović, B., Jelušić, A., Marković, S., Sedlarević Zorić, A.,& Veljović-Jovanović, S.. (2023). ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
University of Belgrade, Technical Faculty in Bor., 135-140.
https://hdl.handle.net/21.15107/rcub_rimsi_1984
Šušić N, Milić Komić S, Živanović B, Jelušić A, Marković S, Sedlarević Zorić A, Veljović-Jovanović S. ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23. 2023;:135-140.
https://hdl.handle.net/21.15107/rcub_rimsi_1984 .
Šušić, Nikola, Milić Komić, Sonja, Živanović, Bojana, Jelušić, Aleksandra, Marković, Sanja, Sedlarević Zorić, Ana, Veljović-Jovanović, Sonja, "ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION" in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23 (2023):135-140,
https://hdl.handle.net/21.15107/rcub_rimsi_1984 .

LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE

Veljović-Jovanović, Sonja; Milić Komić, Sonja; Živanović, Bojana; Sedlarević Zorić, Ana; Šušić, Nikola

(University of Belgrade, Technical Faculty in Bor, 2023)

TY  - CONF
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Sedlarević Zorić, Ana
AU  - Šušić, Nikola
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1983
AB  - Street trees are important component of urban forest presenting a first barrier between air pollution
originated from vehicle traffic and pedestrians. It implies that an improvement of air quality in urban
areas greatly depends on green biomass, in short, the bigger and greener tree crown, better for human
health and wellbeing. Determination of Leaf Nitrogen Balance Index (LNBI) and chlorophyll
concentration (ChlC) by a non-invasive methodology and a user friendly instrument (Dualex 4,
Force), widely used in agronomy and horticulture, was tested here for the assessment of tree fitness in
urban zones. Investment of energy and resources either in growth or defence according to the tradeoff
strategy of plants may be indicated by LNBI, which approximately presents a Nitrogen/Carbon
ratio. We selected few tree species from Belgrade’s streets to determine those two parameters during
summer. We also presented the changes in those parameters of the introduced bamboo species within
ten years at several urban locations in Belgrade differing in air pollution aiming to evaluate
usefulness the LNBI parameter in access of multiyear exposure to the effect of intense vehicle traffic.
Numerous limiting factors for development of healthy tree crowns in urban ecosystem, such as low
capacity to cope with toxic pollutant, sensitivity to diseases, early senescence and etc., greatly depends
on tree species. We propose this methodology may also contribute in the process of choice of the
adequate tree species to be planted along streets.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
T1  - LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE
EP  - 128
SP  - 122
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1983
ER  - 
@conference{
author = "Veljović-Jovanović, Sonja and Milić Komić, Sonja and Živanović, Bojana and Sedlarević Zorić, Ana and Šušić, Nikola",
year = "2023",
abstract = "Street trees are important component of urban forest presenting a first barrier between air pollution
originated from vehicle traffic and pedestrians. It implies that an improvement of air quality in urban
areas greatly depends on green biomass, in short, the bigger and greener tree crown, better for human
health and wellbeing. Determination of Leaf Nitrogen Balance Index (LNBI) and chlorophyll
concentration (ChlC) by a non-invasive methodology and a user friendly instrument (Dualex 4,
Force), widely used in agronomy and horticulture, was tested here for the assessment of tree fitness in
urban zones. Investment of energy and resources either in growth or defence according to the tradeoff
strategy of plants may be indicated by LNBI, which approximately presents a Nitrogen/Carbon
ratio. We selected few tree species from Belgrade’s streets to determine those two parameters during
summer. We also presented the changes in those parameters of the introduced bamboo species within
ten years at several urban locations in Belgrade differing in air pollution aiming to evaluate
usefulness the LNBI parameter in access of multiyear exposure to the effect of intense vehicle traffic.
Numerous limiting factors for development of healthy tree crowns in urban ecosystem, such as low
capacity to cope with toxic pollutant, sensitivity to diseases, early senescence and etc., greatly depends
on tree species. We propose this methodology may also contribute in the process of choice of the
adequate tree species to be planted along streets.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23",
title = "LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE",
pages = "128-122",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1983"
}
Veljović-Jovanović, S., Milić Komić, S., Živanović, B., Sedlarević Zorić, A.,& Šušić, N.. (2023). LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
University of Belgrade, Technical Faculty in Bor., 122-128.
https://hdl.handle.net/21.15107/rcub_rimsi_1983
Veljović-Jovanović S, Milić Komić S, Živanović B, Sedlarević Zorić A, Šušić N. LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23. 2023;:122-128.
https://hdl.handle.net/21.15107/rcub_rimsi_1983 .
Veljović-Jovanović, Sonja, Milić Komić, Sonja, Živanović, Bojana, Sedlarević Zorić, Ana, Šušić, Nikola, "LEAF NITROGEN BALANCE INDEX USED TO MONITOR STRESS RESPONSE TO AIR POLLUTION OF DECIDUOUS TREE SPECIES GROWN IN URBAN ZONE OF BELGRADE" in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23 (2023):122-128,
https://hdl.handle.net/21.15107/rcub_rimsi_1983 .

Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties

Milić Komić, Sonja; Živanović, Bojana; Dumanović, Jelena; kolarž, Predrag; Sedlarević Zorić, Ana; Morina, Filis; Vidović, Marija; Veljović-Jovanović, Sonja

(MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005, 2023)

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Dumanović, Jelena
AU  - kolarž, Predrag
AU  - Sedlarević Zorić, Ana
AU  - Morina, Filis
AU  - Vidović, Marija
AU  - Veljović-Jovanović, Sonja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2220
AB  - Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum x citriodorum, and Ocimum
basilicum var. purpurascens) were grown under moderate light (about 300 umol photons m-2 s-1)
in a glasshouse or growth chamber and then either transferred to an open field (average daily dose:
29.2 kJ m-2 d-1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m-2 d-1),
to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity
(TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine
the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation
at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially
caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a
two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced
preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity,
but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal
flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition
of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated
with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological
changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance
mechanism to high doses of UV-B irradiation.
PB  - MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005
T2  - International Journal of Molecular Sciences
T1  - Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties
VL  - 24(20), 15350
DO  - 10.3390/ijms242015350
ER  - 
@article{
author = "Milić Komić, Sonja and Živanović, Bojana and Dumanović, Jelena and kolarž, Predrag and Sedlarević Zorić, Ana and Morina, Filis and Vidović, Marija and Veljović-Jovanović, Sonja",
year = "2023",
abstract = "Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum x citriodorum, and Ocimum
basilicum var. purpurascens) were grown under moderate light (about 300 umol photons m-2 s-1)
in a glasshouse or growth chamber and then either transferred to an open field (average daily dose:
29.2 kJ m-2 d-1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m-2 d-1),
to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity
(TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine
the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation
at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially
caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a
two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced
preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity,
but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal
flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition
of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated
with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological
changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance
mechanism to high doses of UV-B irradiation.",
publisher = "MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005",
journal = "International Journal of Molecular Sciences",
title = "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties",
volume = "24(20), 15350",
doi = "10.3390/ijms242015350"
}
Milić Komić, S., Živanović, B., Dumanović, J., kolarž, P., Sedlarević Zorić, A., Morina, F., Vidović, M.,& Veljović-Jovanović, S.. (2023). Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences
MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005., 24(20), 15350.
https://doi.org/10.3390/ijms242015350
Milić Komić S, Živanović B, Dumanović J, kolarž P, Sedlarević Zorić A, Morina F, Vidović M, Veljović-Jovanović S. Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences. 2023;24(20), 15350.
doi:10.3390/ijms242015350 .
Milić Komić, Sonja, Živanović, Bojana, Dumanović, Jelena, kolarž, Predrag, Sedlarević Zorić, Ana, Morina, Filis, Vidović, Marija, Veljović-Jovanović, Sonja, "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties" in International Journal of Molecular Sciences, 24(20), 15350 (2023),
https://doi.org/10.3390/ijms242015350 . .
4

Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions

Milić, Dejana; Živanović, Bojana; Samardžić, Jelena; Nikolić, Nenad; Cukier, Caroline; Limami, Anis M.; Vidović, Marija

(MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005, 2023)

TY  - JOUR
AU  - Milić, Dejana
AU  - Živanović, Bojana
AU  - Samardžić, Jelena
AU  - Nikolić, Nenad
AU  - Cukier, Caroline
AU  - Limami, Anis M.
AU  - Vidović, Marija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2390
AB  - Plants are inevitably exposed to extreme climatic conditions that lead to a disturbed balance
between the amount of absorbed energy and their ability to process it. Variegated leaves with
photosynthetically active green leaf tissue (GL) and photosynthetically inactive white leaf tissue
(WL) are an excellent model system to study source–sink interactions within the same leaf under the
same microenvironmental conditions. We demonstrated that under excess excitation energy (EEE)
conditions (high irradiance and lower temperature), regulated metabolic reprogramming in both
leaf tissues allowed an increased consumption of reducing equivalents, as evidenced by preserved
maximum efficiency of photosystem II (φPSII) at the end of the experiment. GL of the EEE-treated
plants employed two strategies: (i) the accumulation of flavonoid glycosides, especially cyanidin
glycosides, as an alternative electron sink, and (ii) cell wall stiffening by cellulose, pectin, and lignin
accumulation. On the other hand, WL increased the amount of free amino acids, mainly arginine,
asparagine, branched-chain and aromatic amino acids, as well as kaempferol and quercetin glycosides.
Thus, WL acts as an important energy escape valve that is required in order to maintain the successful
performance of the GL sectors under EEE conditions. Finally, this role could be an adaptive value of
variegation, as no consistent conclusions about its ecological benefits have been proposed so far.
PB  - MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005
T2  - International Journal of Molecular Sciences
T1  - Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions
EP  - 2269
IS  - 3
SP  - 2269
VL  - 24
DO  - 10.3390/ijms24032269
ER  - 
@article{
author = "Milić, Dejana and Živanović, Bojana and Samardžić, Jelena and Nikolić, Nenad and Cukier, Caroline and Limami, Anis M. and Vidović, Marija",
year = "2023",
abstract = "Plants are inevitably exposed to extreme climatic conditions that lead to a disturbed balance
between the amount of absorbed energy and their ability to process it. Variegated leaves with
photosynthetically active green leaf tissue (GL) and photosynthetically inactive white leaf tissue
(WL) are an excellent model system to study source–sink interactions within the same leaf under the
same microenvironmental conditions. We demonstrated that under excess excitation energy (EEE)
conditions (high irradiance and lower temperature), regulated metabolic reprogramming in both
leaf tissues allowed an increased consumption of reducing equivalents, as evidenced by preserved
maximum efficiency of photosystem II (φPSII) at the end of the experiment. GL of the EEE-treated
plants employed two strategies: (i) the accumulation of flavonoid glycosides, especially cyanidin
glycosides, as an alternative electron sink, and (ii) cell wall stiffening by cellulose, pectin, and lignin
accumulation. On the other hand, WL increased the amount of free amino acids, mainly arginine,
asparagine, branched-chain and aromatic amino acids, as well as kaempferol and quercetin glycosides.
Thus, WL acts as an important energy escape valve that is required in order to maintain the successful
performance of the GL sectors under EEE conditions. Finally, this role could be an adaptive value of
variegation, as no consistent conclusions about its ecological benefits have been proposed so far.",
publisher = "MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005",
journal = "International Journal of Molecular Sciences",
title = "Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions",
pages = "2269-2269",
number = "3",
volume = "24",
doi = "10.3390/ijms24032269"
}
Milić, D., Živanović, B., Samardžić, J., Nikolić, N., Cukier, C., Limami, A. M.,& Vidović, M.. (2023). Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions. in International Journal of Molecular Sciences
MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005., 24(3), 2269-2269.
https://doi.org/10.3390/ijms24032269
Milić D, Živanović B, Samardžić J, Nikolić N, Cukier C, Limami AM, Vidović M. Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions. in International Journal of Molecular Sciences. 2023;24(3):2269-2269.
doi:10.3390/ijms24032269 .
Milić, Dejana, Živanović, Bojana, Samardžić, Jelena, Nikolić, Nenad, Cukier, Caroline, Limami, Anis M., Vidović, Marija, "Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions" in International Journal of Molecular Sciences, 24, no. 3 (2023):2269-2269,
https://doi.org/10.3390/ijms24032269 . .
1

Uticaj ciklusa suše na metabolizam ugljenih hidrata i antioksidanata kod divljeg tipa i flacca mutanta paradajza (Lycopersicum esculentum Mill.) gajenih na različitim svetlosnim režimima

Živanović, Bojana

(Универзитет у Београду, Биолошки факултет, 2022)

TY  - THES
AU  - Živanović, Bojana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1836
AB  - Stres suše, u kombinaciji sa svetlošću visokog intenziteta zračenja, predstavlja jedan od najrasprostranjenijih abiotskih faktora koji ima negativan uticaj na rastenje i prinos biljaka. U cilju ispitivanja uticaja sniženog nivoa apscisinske kiseline (ABA) na razvijanje strategija neophodnih za toleranciju biljaka na sušu, u ovom istraživanju korišćena su dva genotipa paradajza sa konstitutivno različitim sadržajem ABA, divlji tip Ailsa Craig i flacca mutant. Biljke su gajene na svetlosti nižeg i višeg intenziteta i bile podvrgnute jednom, odnosno trima ciklusima suše, koji su bili praćeni periodima rehidratacije. Uticaj suše i rehidratacije praćen je na morfološkom (sveža i suva masa, površina listova) i fiziološkom nivou (provodljivost stoma, ukupni vodni potencijal), kao i primenom biohemijskih (određivanje ugljenih hidrata, aminokiselina, askorbata) i molekularnih metoda (praćenje ekspresije gena uključenih u metabolizam ABA i određivanje ekspresije nekodirajučih RNK molekula).Rezultati prikazani u ovoj disertaciji pokazali su da je održavanje homeostaze ABA od presudnog značaja za rastenje i razviće paradajza i u uslovima optimalnog vodnog režima, kao i da je flacca mutant pogodan model sistem za proučavanje uticaja smanjene akumulacije ABA na razvoj strategija za razvijanje tolerancije biljaka na sušu.Pokazano je da akumulacija osmotski aktivnih jedinjenja kod oba genotipa, kao i promene na morfološkom nivou značajno doprinose razvoju mehanizama tolerancije paradajza na sušu.Izlaganje biljaka ciklusima suše indukovalo je promene u akumulaciji antioksidativnih komponenti (askorbat i epidermalni flavonoidi), strukturi ćelijskog zida, ali i u ekspresiji nekodirajućih RNK molekula, što potencijalno može doprineti razvijanju memorije kod paradajza na ponovljeni stres suše.
AB  - Drought stress combined with high light intensity is one of the most common abiotic factors that have a negative impact on plant growth and yield. In order to examine the effect of reduced abscisic acid (ABA) level in the development strategies necessary for drought tolerance in plants, tomato genotypes with constitutively different ABA content, wild type Ailsa Craig and flacca mutant, were used in this study. Plants grown on either lower or higher light intensity were subjected to one or three drought cycles, followed by periods of rehydration. The impact of drought and rehydration was monitored on morphological (fresh and dry weight, leaf area) and physiological level (stomatal conductivity, leaf water potential), and by measuring biochemical (carbohydrates, amino acids, ascorbate) and molecular parameters (expression of genes involved in ABA metabolism and expression of noncoding RNA molecules).The results obtained in this dissertation showed that maintaining ABA homeostasis is crucial for tomato growth and development, even in optimal water regimes, and also that flacca mutant is a suitable model system for studying the impact of reduced ABA accumulation in development of drought tolerance strategies.It has been noticed that morphological changes and osmolyte accumulation in both genotypes significantly contribute in developing drought tolerance mechanisms of tomato plants.Exposure of plants to several drought cycles has induced changes in the accumulation of antioxidant components (ascorbate and epidermal flavonoids), cell wall structure, but also in the expression of non-coding RNA molecules, which could potentially contribute to memory development in tomatoes.
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Uticaj ciklusa suše na metabolizam ugljenih hidrata i antioksidanata kod divljeg tipa i flacca mutanta paradajza (Lycopersicum esculentum Mill.) gajenih na različitim svetlosnim režimima
UR  - https://hdl.handle.net/21.15107/rcub_nardus_21094
ER  - 
@phdthesis{
author = "Živanović, Bojana",
year = "2022",
abstract = "Stres suše, u kombinaciji sa svetlošću visokog intenziteta zračenja, predstavlja jedan od najrasprostranjenijih abiotskih faktora koji ima negativan uticaj na rastenje i prinos biljaka. U cilju ispitivanja uticaja sniženog nivoa apscisinske kiseline (ABA) na razvijanje strategija neophodnih za toleranciju biljaka na sušu, u ovom istraživanju korišćena su dva genotipa paradajza sa konstitutivno različitim sadržajem ABA, divlji tip Ailsa Craig i flacca mutant. Biljke su gajene na svetlosti nižeg i višeg intenziteta i bile podvrgnute jednom, odnosno trima ciklusima suše, koji su bili praćeni periodima rehidratacije. Uticaj suše i rehidratacije praćen je na morfološkom (sveža i suva masa, površina listova) i fiziološkom nivou (provodljivost stoma, ukupni vodni potencijal), kao i primenom biohemijskih (određivanje ugljenih hidrata, aminokiselina, askorbata) i molekularnih metoda (praćenje ekspresije gena uključenih u metabolizam ABA i određivanje ekspresije nekodirajučih RNK molekula).Rezultati prikazani u ovoj disertaciji pokazali su da je održavanje homeostaze ABA od presudnog značaja za rastenje i razviće paradajza i u uslovima optimalnog vodnog režima, kao i da je flacca mutant pogodan model sistem za proučavanje uticaja smanjene akumulacije ABA na razvoj strategija za razvijanje tolerancije biljaka na sušu.Pokazano je da akumulacija osmotski aktivnih jedinjenja kod oba genotipa, kao i promene na morfološkom nivou značajno doprinose razvoju mehanizama tolerancije paradajza na sušu.Izlaganje biljaka ciklusima suše indukovalo je promene u akumulaciji antioksidativnih komponenti (askorbat i epidermalni flavonoidi), strukturi ćelijskog zida, ali i u ekspresiji nekodirajućih RNK molekula, što potencijalno može doprineti razvijanju memorije kod paradajza na ponovljeni stres suše., Drought stress combined with high light intensity is one of the most common abiotic factors that have a negative impact on plant growth and yield. In order to examine the effect of reduced abscisic acid (ABA) level in the development strategies necessary for drought tolerance in plants, tomato genotypes with constitutively different ABA content, wild type Ailsa Craig and flacca mutant, were used in this study. Plants grown on either lower or higher light intensity were subjected to one or three drought cycles, followed by periods of rehydration. The impact of drought and rehydration was monitored on morphological (fresh and dry weight, leaf area) and physiological level (stomatal conductivity, leaf water potential), and by measuring biochemical (carbohydrates, amino acids, ascorbate) and molecular parameters (expression of genes involved in ABA metabolism and expression of noncoding RNA molecules).The results obtained in this dissertation showed that maintaining ABA homeostasis is crucial for tomato growth and development, even in optimal water regimes, and also that flacca mutant is a suitable model system for studying the impact of reduced ABA accumulation in development of drought tolerance strategies.It has been noticed that morphological changes and osmolyte accumulation in both genotypes significantly contribute in developing drought tolerance mechanisms of tomato plants.Exposure of plants to several drought cycles has induced changes in the accumulation of antioxidant components (ascorbate and epidermal flavonoids), cell wall structure, but also in the expression of non-coding RNA molecules, which could potentially contribute to memory development in tomatoes.",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Uticaj ciklusa suše na metabolizam ugljenih hidrata i antioksidanata kod divljeg tipa i flacca mutanta paradajza (Lycopersicum esculentum Mill.) gajenih na različitim svetlosnim režimima",
url = "https://hdl.handle.net/21.15107/rcub_nardus_21094"
}
Živanović, B.. (2022). Uticaj ciklusa suše na metabolizam ugljenih hidrata i antioksidanata kod divljeg tipa i flacca mutanta paradajza (Lycopersicum esculentum Mill.) gajenih na različitim svetlosnim režimima. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_21094
Živanović B. Uticaj ciklusa suše na metabolizam ugljenih hidrata i antioksidanata kod divljeg tipa i flacca mutanta paradajza (Lycopersicum esculentum Mill.) gajenih na različitim svetlosnim režimima. in Универзитет у Београду. 2022;.
https://hdl.handle.net/21.15107/rcub_nardus_21094 .
Živanović, Bojana, "Uticaj ciklusa suše na metabolizam ugljenih hidrata i antioksidanata kod divljeg tipa i flacca mutanta paradajza (Lycopersicum esculentum Mill.) gajenih na različitim svetlosnim režimima" in Универзитет у Београду (2022),
https://hdl.handle.net/21.15107/rcub_nardus_21094 .

Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca

Živanović, Bojana; Prokić, Ljiljana; Milić Komić, Sonja; Nikolić, Nenad; Sedlarević Zorić, Ana; Vidović, Marija; Veljović-Jovanović, Sonja

(Serbian Plant Physiology Society, 2022)

TY  - CONF
AU  - Živanović, Bojana
AU  - Prokić, Ljiljana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1871
AB  - The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1871
ER  - 
@conference{
author = "Živanović, Bojana and Prokić, Ljiljana and Milić Komić, Sonja and Nikolić, Nenad and Sedlarević Zorić, Ana and Vidović, Marija and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1871"
}
Živanović, B., Prokić, L., Milić Komić, S., Nikolić, N., Sedlarević Zorić, A., Vidović, M.,& Veljović-Jovanović, S.. (2022). Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Serbian Plant Physiology Society., 83.
https://hdl.handle.net/21.15107/rcub_rimsi_1871
Živanović B, Prokić L, Milić Komić S, Nikolić N, Sedlarević Zorić A, Vidović M, Veljović-Jovanović S. Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;:83.
https://hdl.handle.net/21.15107/rcub_rimsi_1871 .
Živanović, Bojana, Prokić, Ljiljana, Milić Komić, Sonja, Nikolić, Nenad, Sedlarević Zorić, Ana, Vidović, Marija, Veljović-Jovanović, Sonja, "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca" in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022):83,
https://hdl.handle.net/21.15107/rcub_rimsi_1871 .

The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought

Golob, Aleksandra; Ojdanič, Nik; Živanović, Bojana; Germ, Mateja; Milić Komić, Sonja; Sedlarević Zorić, Ana; Milić, Dejana; Pantelić, Ana; Mavrič Čermelj, Anja; Samardžić, Jelena; Veljović-Jovanović, Sonja; Vidović, Marija

(Serbian Plant Physiology Society, 2022)

TY  - CONF
AU  - Golob, Aleksandra
AU  - Ojdanič, Nik
AU  - Živanović, Bojana
AU  - Germ, Mateja
AU  - Milić Komić, Sonja
AU  - Sedlarević Zorić, Ana
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Mavrič Čermelj, Anja
AU  - Samardžić, Jelena
AU  - Veljović-Jovanović, Sonja
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1872
AB  - Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered a
beneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperatures
by promoting antioxidant production. However, the underlying mechanisms are still not
elucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,
leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed to
water shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;
(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impacts
photochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflective
and transmissive properties. Si application between flag leaf emergence and grain filling
had the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was the
most abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was the
only hydroxycinnamic acid showing significant accumulation with the latest applied Si compared
to no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,
aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to drought
without Si addition, while lysine was the most accumulated in the leaves supplemented by Si at
the grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to drought
regardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, its
effect was dependent on the barley growth phase prior to supplementation.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought
SP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1872
ER  - 
@conference{
author = "Golob, Aleksandra and Ojdanič, Nik and Živanović, Bojana and Germ, Mateja and Milić Komić, Sonja and Sedlarević Zorić, Ana and Milić, Dejana and Pantelić, Ana and Mavrič Čermelj, Anja and Samardžić, Jelena and Veljović-Jovanović, Sonja and Vidović, Marija",
year = "2022",
abstract = "Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered a
beneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperatures
by promoting antioxidant production. However, the underlying mechanisms are still not
elucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,
leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed to
water shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;
(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impacts
photochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflective
and transmissive properties. Si application between flag leaf emergence and grain filling
had the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was the
most abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was the
only hydroxycinnamic acid showing significant accumulation with the latest applied Si compared
to no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,
aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to drought
without Si addition, while lysine was the most accumulated in the leaves supplemented by Si at
the grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to drought
regardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, its
effect was dependent on the barley growth phase prior to supplementation.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought",
pages = "67",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1872"
}
Golob, A., Ojdanič, N., Živanović, B., Germ, M., Milić Komić, S., Sedlarević Zorić, A., Milić, D., Pantelić, A., Mavrič Čermelj, A., Samardžić, J., Veljović-Jovanović, S.,& Vidović, M.. (2022). The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Serbian Plant Physiology Society., 67.
https://hdl.handle.net/21.15107/rcub_rimsi_1872
Golob A, Ojdanič N, Živanović B, Germ M, Milić Komić S, Sedlarević Zorić A, Milić D, Pantelić A, Mavrič Čermelj A, Samardžić J, Veljović-Jovanović S, Vidović M. The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;:67.
https://hdl.handle.net/21.15107/rcub_rimsi_1872 .
Golob, Aleksandra, Ojdanič, Nik, Živanović, Bojana, Germ, Mateja, Milić Komić, Sonja, Sedlarević Zorić, Ana, Milić, Dejana, Pantelić, Ana, Mavrič Čermelj, Anja, Samardžić, Jelena, Veljović-Jovanović, Sonja, Vidović, Marija, "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought" in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022):67,
https://hdl.handle.net/21.15107/rcub_rimsi_1872 .

Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties

Milić Komić, Sonja; Živanović, Bojana; Sedlarević Zorić, Ana; Vidović, Marija; Veljović-Jovanović, Sonja

(Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade, 2022)

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1873
AB  - Three varieties of basil (Ocimum x citriodorum, Ocimum basilicum var. Genovese and Ocimum
basilicum var. purpurascens) were used to examine the effect of different PAR intensities (100, 400
and 1400 µmol photons m-2s-1), as well as different UV-B/PAR ratios, on leaf phenolics accumulation, and components of the ascorbate-glutathione cycle. Phenolic compounds represent the
most abundant class of secondary metabolites, and their function in plants involves protection
against numerous abiotic stresses, role in growth and development, flowering, reproduction and
seed dispersion. A preferential accumulation of HCAs over Flav, induction of class III POD activity
and decreased ascorbate content were characteristic responses to pro-oxidative effect of the high
UV-B/PAR ratio in all three basil varieties. The most remarkable result was the lack of accumulation
of epidermal UV absorbing substances, which appeared to be a crucial photoprotective mechanism in sunlight. The contrasting effect of ecologically relevant UV-B radiation on basil plants,
pro-oxidative vs. acclimative, was determined by the quality and intensity of the background light.
Acclimation of basil to high light comprises a number of processes, among which are the accumulation of epidermal flavonoids and total leaf phenolics, antioxidant response (increase in Asc and
GSH) and lack of downregulation of PSII upon increasing light intensity. Among varieties that we
used as model system in this study, purple basil, with the highest constitutive amount of anthocyanidins, showed the least induction of epidermal flavonoids and lack of light effect upon transfer
from initial 50 to 250 µmol photons m-2s-1.
PB  - Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology (23rd SPPS Meeting)
T1  - Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1873
ER  - 
@conference{
author = "Milić Komić, Sonja and Živanović, Bojana and Sedlarević Zorić, Ana and Vidović, Marija and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "Three varieties of basil (Ocimum x citriodorum, Ocimum basilicum var. Genovese and Ocimum
basilicum var. purpurascens) were used to examine the effect of different PAR intensities (100, 400
and 1400 µmol photons m-2s-1), as well as different UV-B/PAR ratios, on leaf phenolics accumulation, and components of the ascorbate-glutathione cycle. Phenolic compounds represent the
most abundant class of secondary metabolites, and their function in plants involves protection
against numerous abiotic stresses, role in growth and development, flowering, reproduction and
seed dispersion. A preferential accumulation of HCAs over Flav, induction of class III POD activity
and decreased ascorbate content were characteristic responses to pro-oxidative effect of the high
UV-B/PAR ratio in all three basil varieties. The most remarkable result was the lack of accumulation
of epidermal UV absorbing substances, which appeared to be a crucial photoprotective mechanism in sunlight. The contrasting effect of ecologically relevant UV-B radiation on basil plants,
pro-oxidative vs. acclimative, was determined by the quality and intensity of the background light.
Acclimation of basil to high light comprises a number of processes, among which are the accumulation of epidermal flavonoids and total leaf phenolics, antioxidant response (increase in Asc and
GSH) and lack of downregulation of PSII upon increasing light intensity. Among varieties that we
used as model system in this study, purple basil, with the highest constitutive amount of anthocyanidins, showed the least induction of epidermal flavonoids and lack of light effect upon transfer
from initial 50 to 250 µmol photons m-2s-1.",
publisher = "Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology (23rd SPPS Meeting)",
title = "Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1873"
}
Milić Komić, S., Živanović, B., Sedlarević Zorić, A., Vidović, M.,& Veljović-Jovanović, S.. (2022). Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties. in 4th International Conference on Plant Biology (23rd SPPS Meeting)
Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_rimsi_1873
Milić Komić S, Živanović B, Sedlarević Zorić A, Vidović M, Veljović-Jovanović S. Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties. in 4th International Conference on Plant Biology (23rd SPPS Meeting). 2022;:43.
https://hdl.handle.net/21.15107/rcub_rimsi_1873 .
Milić Komić, Sonja, Živanović, Bojana, Sedlarević Zorić, Ana, Vidović, Marija, Veljović-Jovanović, Sonja, "Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties" in 4th International Conference on Plant Biology (23rd SPPS Meeting) (2022):43,
https://hdl.handle.net/21.15107/rcub_rimsi_1873 .

Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles

Živanović, Bojana; Milić Komić, Sonja; Nikolić, Nenad; Mutavdžić, Dragosav; Srećković, Tatjana; Veljović-Jovanović, Sonja; Prokic, Ljiljana

(MDPI, Basel, 2021)

TY  - JOUR
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Mutavdžić, Dragosav
AU  - Srećković, Tatjana
AU  - Veljović-Jovanović, Sonja
AU  - Prokic, Ljiljana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1509
AB  - Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles
IS  - 11
VL  - 10
DO  - 10.3390/plants10112308
ER  - 
@article{
author = "Živanović, Bojana and Milić Komić, Sonja and Nikolić, Nenad and Mutavdžić, Dragosav and Srećković, Tatjana and Veljović-Jovanović, Sonja and Prokic, Ljiljana",
year = "2021",
abstract = "Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles",
number = "11",
volume = "10",
doi = "10.3390/plants10112308"
}
Živanović, B., Milić Komić, S., Nikolić, N., Mutavdžić, D., Srećković, T., Veljović-Jovanović, S.,& Prokic, L.. (2021). Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles. in Plants-Basel
MDPI, Basel., 10(11).
https://doi.org/10.3390/plants10112308
Živanović B, Milić Komić S, Nikolić N, Mutavdžić D, Srećković T, Veljović-Jovanović S, Prokic L. Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles. in Plants-Basel. 2021;10(11).
doi:10.3390/plants10112308 .
Živanović, Bojana, Milić Komić, Sonja, Nikolić, Nenad, Mutavdžić, Dragosav, Srećković, Tatjana, Veljović-Jovanović, Sonja, Prokic, Ljiljana, "Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles" in Plants-Basel, 10, no. 11 (2021),
https://doi.org/10.3390/plants10112308 . .
1
5
5

Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato

Živanović, Bojana; Milić Komić, Sonja; Tosti, Tomislav; Vidović, Marija; Prokic, Ljiljana; Veljović-Jovanović, Sonja

(MDPI, Basel, 2020)

TY  - JOUR
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Tosti, Tomislav
AU  - Vidović, Marija
AU  - Prokic, Ljiljana
AU  - Veljović-Jovanović, Sonja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1347
AB  - Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato
IS  - 9
VL  - 9
DO  - 10.3390/plants9091147
ER  - 
@article{
author = "Živanović, Bojana and Milić Komić, Sonja and Tosti, Tomislav and Vidović, Marija and Prokic, Ljiljana and Veljović-Jovanović, Sonja",
year = "2020",
abstract = "Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato",
number = "9",
volume = "9",
doi = "10.3390/plants9091147"
}
Živanović, B., Milić Komić, S., Tosti, T., Vidović, M., Prokic, L.,& Veljović-Jovanović, S.. (2020). Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel
MDPI, Basel., 9(9).
https://doi.org/10.3390/plants9091147
Živanović B, Milić Komić S, Tosti T, Vidović M, Prokic L, Veljović-Jovanović S. Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel. 2020;9(9).
doi:10.3390/plants9091147 .
Živanović, Bojana, Milić Komić, Sonja, Tosti, Tomislav, Vidović, Marija, Prokic, Ljiljana, Veljović-Jovanović, Sonja, "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato" in Plants-Basel, 9, no. 9 (2020),
https://doi.org/10.3390/plants9091147 . .
2
43
10
41

Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates

Živanović, Bojana; Vidović, Marija; Milić Komić, Sonja; Jovanović, Ljubinko; Kolarz, Predrag; Morina, Filis; Veljović-Jovanović, Sonja

(Tubitak Scientific & Technical Research Council Turkey, Ankara, 2017)

TY  - JOUR
AU  - Živanović, Bojana
AU  - Vidović, Marija
AU  - Milić Komić, Sonja
AU  - Jovanović, Ljubinko
AU  - Kolarz, Predrag
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1066
AB  - Tomato (Solanum lycopersicum L.) is among the economically most important vegetables in Europe, valued for its bioactive properties due to significant contents of vitamins, carotenoids, and phenolic compounds. In this study, the tomato cultivar Big Beef F1 was grown in the open field (OF) and under polytunnels in central Serbia during 3 years. Polytunnels were covered with two foils (both with 57% reduced photosynthetic active radiation, PAR) differing in UV-A and UV-B transmittance. The aim of our work was to determine the influence of light conditions on accumulation of phytonutrients (carotenoids and phenolics) in the peel and flesh of ripe tomato fruits. The amount of effective antioxidants, caffeic acid, and quercetin (phenolics with ortho-dihydroxy substitution) in the peel was the highest in tomato fruits grown in the OF (maximal PAR and UV-A and UV-B radiation). Moreover, the content of leaf epidermal flavonoids was the highest in the OF. The content of lycopene and beta-carotene in the flesh of tomato fruit was higher under the polytunnel with higher UV-transmittance. Our results showed that selection of the right light conditions (quality and intensity) for tomato production has a significant effect on the accumulation of beneficial phenolics and carotenoids.
PB  - Tubitak Scientific & Technical Research Council Turkey, Ankara
T2  - Turkish Journal of Agriculture and Forestry
T1  - Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates
EP  - +
IS  - 2
SP  - 113
VL  - 41
DO  - 10.3906/tar-1612-56
ER  - 
@article{
author = "Živanović, Bojana and Vidović, Marija and Milić Komić, Sonja and Jovanović, Ljubinko and Kolarz, Predrag and Morina, Filis and Veljović-Jovanović, Sonja",
year = "2017",
abstract = "Tomato (Solanum lycopersicum L.) is among the economically most important vegetables in Europe, valued for its bioactive properties due to significant contents of vitamins, carotenoids, and phenolic compounds. In this study, the tomato cultivar Big Beef F1 was grown in the open field (OF) and under polytunnels in central Serbia during 3 years. Polytunnels were covered with two foils (both with 57% reduced photosynthetic active radiation, PAR) differing in UV-A and UV-B transmittance. The aim of our work was to determine the influence of light conditions on accumulation of phytonutrients (carotenoids and phenolics) in the peel and flesh of ripe tomato fruits. The amount of effective antioxidants, caffeic acid, and quercetin (phenolics with ortho-dihydroxy substitution) in the peel was the highest in tomato fruits grown in the OF (maximal PAR and UV-A and UV-B radiation). Moreover, the content of leaf epidermal flavonoids was the highest in the OF. The content of lycopene and beta-carotene in the flesh of tomato fruit was higher under the polytunnel with higher UV-transmittance. Our results showed that selection of the right light conditions (quality and intensity) for tomato production has a significant effect on the accumulation of beneficial phenolics and carotenoids.",
publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara",
journal = "Turkish Journal of Agriculture and Forestry",
title = "Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates",
pages = "+-113",
number = "2",
volume = "41",
doi = "10.3906/tar-1612-56"
}
Živanović, B., Vidović, M., Milić Komić, S., Jovanović, L., Kolarz, P., Morina, F.,& Veljović-Jovanović, S.. (2017). Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates. in Turkish Journal of Agriculture and Forestry
Tubitak Scientific & Technical Research Council Turkey, Ankara., 41(2), 113-+.
https://doi.org/10.3906/tar-1612-56
Živanović B, Vidović M, Milić Komić S, Jovanović L, Kolarz P, Morina F, Veljović-Jovanović S. Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates. in Turkish Journal of Agriculture and Forestry. 2017;41(2):113-+.
doi:10.3906/tar-1612-56 .
Živanović, Bojana, Vidović, Marija, Milić Komić, Sonja, Jovanović, Ljubinko, Kolarz, Predrag, Morina, Filis, Veljović-Jovanović, Sonja, "Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates" in Turkish Journal of Agriculture and Forestry, 41, no. 2 (2017):113-+,
https://doi.org/10.3906/tar-1612-56 . .
18
5
17

Differential dynamics of flavonoid biosynthesis and accumulation in six medicinal herbs under full sunlight exposure

Živanović, Bojana; Sedlarević Zorić, Ana; Vidović, Marija; Morina, Filis; Veljović-Jovanović, Sonja

(Department of Plant Biology, University of Pécs, 2016)

TY  - CONF
AU  - Živanović, Bojana
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1874
AB  - The dynamics of epidermal flavonoid (epFlav) induction was monitored during 11 days in the leaves of three Ocimum basilicum varieties: Americanum, Genovese and Purpurescens, Salvia officinalis, Eruca sativa and Verbascum thapsus previously grown in a glasshouse (receiving 16% of sunlight irradiance without UV). At full sunlight plants received mean daily doses of 6.97 MJ m-2, 0.86 MJ m-2 and 22.46 kJ m-2 in the photosynthetically active radiation, UV-A and UV-B wavelengths, respectively. Although constitutive concentration of epFlav was similar, dynamics of their induction was species-specific. Significant increase in epFlav accumulation was observed 30h after exposure to sunlight in all species except O. basilicum var. Purpurescens, in which the increase was delayed. This delay was correlated with induction of constitutive epidermal anthocyanins. Total leaf flavonoids were unchanged, in contrast to epidermal, emphasizing their UV-B absorbing function. Total antioxidative capacity increased at least 2-fold under sunlight, which was in accordance with total phenolic increase. Leaf dry biomass was 50-100% higher in sunlight-exposed compared to glasshouse grown plants, while fresh biomass was unchanged, indicative of leaf thickening. We showed that flavonoids accumulation was dependent on light conditions and constitutive pool of UV-B absorbing compounds.
PB  - Department of Plant Biology, University of Pécs
C3  - 1st Network Meeting of UV4Plants, International Association for Plant UV Research abstract book, 30 – 31 May, 2016, Pécs, Hungary
T1  - Differential dynamics of flavonoid biosynthesis and accumulation in six medicinal herbs under full sunlight exposure
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1874
ER  - 
@conference{
author = "Živanović, Bojana and Sedlarević Zorić, Ana and Vidović, Marija and Morina, Filis and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "The dynamics of epidermal flavonoid (epFlav) induction was monitored during 11 days in the leaves of three Ocimum basilicum varieties: Americanum, Genovese and Purpurescens, Salvia officinalis, Eruca sativa and Verbascum thapsus previously grown in a glasshouse (receiving 16% of sunlight irradiance without UV). At full sunlight plants received mean daily doses of 6.97 MJ m-2, 0.86 MJ m-2 and 22.46 kJ m-2 in the photosynthetically active radiation, UV-A and UV-B wavelengths, respectively. Although constitutive concentration of epFlav was similar, dynamics of their induction was species-specific. Significant increase in epFlav accumulation was observed 30h after exposure to sunlight in all species except O. basilicum var. Purpurescens, in which the increase was delayed. This delay was correlated with induction of constitutive epidermal anthocyanins. Total leaf flavonoids were unchanged, in contrast to epidermal, emphasizing their UV-B absorbing function. Total antioxidative capacity increased at least 2-fold under sunlight, which was in accordance with total phenolic increase. Leaf dry biomass was 50-100% higher in sunlight-exposed compared to glasshouse grown plants, while fresh biomass was unchanged, indicative of leaf thickening. We showed that flavonoids accumulation was dependent on light conditions and constitutive pool of UV-B absorbing compounds.",
publisher = "Department of Plant Biology, University of Pécs",
journal = "1st Network Meeting of UV4Plants, International Association for Plant UV Research abstract book, 30 – 31 May, 2016, Pécs, Hungary",
title = "Differential dynamics of flavonoid biosynthesis and accumulation in six medicinal herbs under full sunlight exposure",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1874"
}
Živanović, B., Sedlarević Zorić, A., Vidović, M., Morina, F.,& Veljović-Jovanović, S.. (2016). Differential dynamics of flavonoid biosynthesis and accumulation in six medicinal herbs under full sunlight exposure. in 1st Network Meeting of UV4Plants, International Association for Plant UV Research abstract book, 30 – 31 May, 2016, Pécs, Hungary
Department of Plant Biology, University of Pécs., 65.
https://hdl.handle.net/21.15107/rcub_rimsi_1874
Živanović B, Sedlarević Zorić A, Vidović M, Morina F, Veljović-Jovanović S. Differential dynamics of flavonoid biosynthesis and accumulation in six medicinal herbs under full sunlight exposure. in 1st Network Meeting of UV4Plants, International Association for Plant UV Research abstract book, 30 – 31 May, 2016, Pécs, Hungary. 2016;:65.
https://hdl.handle.net/21.15107/rcub_rimsi_1874 .
Živanović, Bojana, Sedlarević Zorić, Ana, Vidović, Marija, Morina, Filis, Veljović-Jovanović, Sonja, "Differential dynamics of flavonoid biosynthesis and accumulation in six medicinal herbs under full sunlight exposure" in 1st Network Meeting of UV4Plants, International Association for Plant UV Research abstract book, 30 – 31 May, 2016, Pécs, Hungary (2016):65,
https://hdl.handle.net/21.15107/rcub_rimsi_1874 .

Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat

Vidović, Marija; Morina, Filis; Prokic, Ljiljana; Milić Komić, Sonja; Živanović, Bojana; Veljović-Jovanović, Sonja

(Elsevier Gmbh, Munich, 2016)

TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Prokic, Ljiljana
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/999
AB  - In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat
EP  - 39
SP  - 25
VL  - 206
DO  - 10.1016/j.jplph.2016.07.017
ER  - 
@article{
author = "Vidović, Marija and Morina, Filis and Prokic, Ljiljana and Milić Komić, Sonja and Živanović, Bojana and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat",
pages = "39-25",
volume = "206",
doi = "10.1016/j.jplph.2016.07.017"
}
Vidović, M., Morina, F., Prokic, L., Milić Komić, S., Živanović, B.,& Veljović-Jovanović, S.. (2016). Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 206, 25-39.
https://doi.org/10.1016/j.jplph.2016.07.017
Vidović M, Morina F, Prokic L, Milić Komić S, Živanović B, Veljović-Jovanović S. Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology. 2016;206:25-39.
doi:10.1016/j.jplph.2016.07.017 .
Vidović, Marija, Morina, Filis, Prokic, Ljiljana, Milić Komić, Sonja, Živanović, Bojana, Veljović-Jovanović, Sonja, "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat" in Journal of Plant Physiology, 206 (2016):25-39,
https://doi.org/10.1016/j.jplph.2016.07.017 . .
13
8
13

Behavioural and physiological plasticity of gypsy moth larvae to host plant switching

Milanović, Slobodan; Jankovic-Tomanic, Milena; Kostić, Igor; Kostic, Miroslav; Morina, Filis; Živanović, Bojana; Lazarević, Jelica

(Wiley, Hoboken, 2016)

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Jankovic-Tomanic, Milena
AU  - Kostić, Igor
AU  - Kostic, Miroslav
AU  - Morina, Filis
AU  - Živanović, Bojana
AU  - Lazarević, Jelica
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/990
AB  - Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.
PB  - Wiley, Hoboken
T2  - Entomologia Experimentalis Et Applicata
T1  - Behavioural and physiological plasticity of gypsy moth larvae to host plant switching
EP  - 162
IS  - 2
SP  - 152
VL  - 158
DO  - 10.1111/eea.12388
ER  - 
@article{
author = "Milanović, Slobodan and Jankovic-Tomanic, Milena and Kostić, Igor and Kostic, Miroslav and Morina, Filis and Živanović, Bojana and Lazarević, Jelica",
year = "2016",
abstract = "Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.",
publisher = "Wiley, Hoboken",
journal = "Entomologia Experimentalis Et Applicata",
title = "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching",
pages = "162-152",
number = "2",
volume = "158",
doi = "10.1111/eea.12388"
}
Milanović, S., Jankovic-Tomanic, M., Kostić, I., Kostic, M., Morina, F., Živanović, B.,& Lazarević, J.. (2016). Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata
Wiley, Hoboken., 158(2), 152-162.
https://doi.org/10.1111/eea.12388
Milanović S, Jankovic-Tomanic M, Kostić I, Kostic M, Morina F, Živanović B, Lazarević J. Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata. 2016;158(2):152-162.
doi:10.1111/eea.12388 .
Milanović, Slobodan, Jankovic-Tomanic, Milena, Kostić, Igor, Kostic, Miroslav, Morina, Filis, Živanović, Bojana, Lazarević, Jelica, "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching" in Entomologia Experimentalis Et Applicata, 158, no. 2 (2016):152-162,
https://doi.org/10.1111/eea.12388 . .
13
8
14

Influence of UV radiation on the content of secondary metabolites in tomato grown in different environmental conditions

Živanović, Bojana; Sedlarević Zorić, Ana; Milić Komić, Sonja; Vidović, Marija; Morina, Filis; Veljović-Jovanović, Sonja

(Serbian Plant Physiology Society, 2015)

TY  - CONF
AU  - Živanović, Bojana
AU  - Sedlarević Zorić, Ana
AU  - Milić Komić, Sonja
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1876
AB  - In this study we investigated the effects of ambient ultraviolet (UV, 280- 400 nm) radiation on the composition
of secondary metabolites of high nutritional value (pigments and flavonoids) in Lycopersicon esculentum (tomato) fruits. Tomato plants were grown during summer in the open field and two types of polytunnels, PT1 ( UV-A 1.45 mV cm-2, UV-B 3.84 μV cm-2, PAR 750 μmol m-2 s-1) and PT2 (UV-A 0.37 mV cm-2, UV-B 0.03 μV cm-2, PAR 760 μmol m-2 s-1) and fruits were taken in august. The contents of lycopene, β-carotene and flavonoids (quercetin and kaempferol) were determined by HPLC in the tomato exocarp and pulp. Regardless of UV radiation exposure, higher amounts of lycopene, flavonoids and β-carotene were measured in exocarp compared to the pulp. Accumulation of phenolics, in both exocarp and pulp was the highest in fruits collected in the field. Similarly, the concentration of epidermal flavonoids was the highest in the leaves of plants from the open field. These results support the protective functions of flavonoids as UV-screeners and antioxidants from
high PAR. Moreover, the content of lycopene was the highest in exocarp and β-carotene in the pulp of fruits exposed to full ambiental UV radiation doses, compared to fruits collected in the polytunells. The results implicate that the controlled exposure to solar UV radiation during crop growth may be used as a stimulator of biosynthesis of compounds with high antioxidative capacity thus improving the fruit quality and nutritional value.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
C3  - 2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015
T1  - Influence of UV radiation on the content of secondary metabolites in tomato grown in different environmental conditions
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1876
ER  - 
@conference{
author = "Živanović, Bojana and Sedlarević Zorić, Ana and Milić Komić, Sonja and Vidović, Marija and Morina, Filis and Veljović-Jovanović, Sonja",
year = "2015",
abstract = "In this study we investigated the effects of ambient ultraviolet (UV, 280- 400 nm) radiation on the composition
of secondary metabolites of high nutritional value (pigments and flavonoids) in Lycopersicon esculentum (tomato) fruits. Tomato plants were grown during summer in the open field and two types of polytunnels, PT1 ( UV-A 1.45 mV cm-2, UV-B 3.84 μV cm-2, PAR 750 μmol m-2 s-1) and PT2 (UV-A 0.37 mV cm-2, UV-B 0.03 μV cm-2, PAR 760 μmol m-2 s-1) and fruits were taken in august. The contents of lycopene, β-carotene and flavonoids (quercetin and kaempferol) were determined by HPLC in the tomato exocarp and pulp. Regardless of UV radiation exposure, higher amounts of lycopene, flavonoids and β-carotene were measured in exocarp compared to the pulp. Accumulation of phenolics, in both exocarp and pulp was the highest in fruits collected in the field. Similarly, the concentration of epidermal flavonoids was the highest in the leaves of plants from the open field. These results support the protective functions of flavonoids as UV-screeners and antioxidants from
high PAR. Moreover, the content of lycopene was the highest in exocarp and β-carotene in the pulp of fruits exposed to full ambiental UV radiation doses, compared to fruits collected in the polytunells. The results implicate that the controlled exposure to solar UV radiation during crop growth may be used as a stimulator of biosynthesis of compounds with high antioxidative capacity thus improving the fruit quality and nutritional value.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade",
journal = "2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015",
title = "Influence of UV radiation on the content of secondary metabolites in tomato grown in different environmental conditions",
pages = "186",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1876"
}
Živanović, B., Sedlarević Zorić, A., Milić Komić, S., Vidović, M., Morina, F.,& Veljović-Jovanović, S.. (2015). Influence of UV radiation on the content of secondary metabolites in tomato grown in different environmental conditions. in 2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015
Serbian Plant Physiology Society., 186.
https://hdl.handle.net/21.15107/rcub_rimsi_1876
Živanović B, Sedlarević Zorić A, Milić Komić S, Vidović M, Morina F, Veljović-Jovanović S. Influence of UV radiation on the content of secondary metabolites in tomato grown in different environmental conditions. in 2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015. 2015;:186.
https://hdl.handle.net/21.15107/rcub_rimsi_1876 .
Živanović, Bojana, Sedlarević Zorić, Ana, Milić Komić, Sonja, Vidović, Marija, Morina, Filis, Veljović-Jovanović, Sonja, "Influence of UV radiation on the content of secondary metabolites in tomato grown in different environmental conditions" in 2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015 (2015):186,
https://hdl.handle.net/21.15107/rcub_rimsi_1876 .