TY  - JOUR
AU  - Stefanović, Slobodan
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Bartolić, Dragana
AU  - Đikanović, Daniela
AU  - Simonović Radosavljević, Jasna
AU  - Mutavdžić, Dragosav
AU  - Radotić, Ksenija
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2306
AB  - In nature, woody substrates are usually degraded by entire communities of microorganisms, which are nowadays jeopardised by anthropogenic influence, making it important to define the functional specificity of every species. Two strains of autochthonous fungi from Serbian lowland forests (Pleurotus ostreatus Ser1 and Cyclocybe aegerita Ser1) have been investigated for their ability to degrade lignin substrates [oak sawdust, oak isolated cell walls, and synthetic dehydrogenative polymer (DHP)]. Measuring the activities of the enzymes involved in lignin degradation was coupled with detecting the HPLC profile of the phenolics in the fungal growth media, and the lignin loss. While Pleurotus ostreatus Ser1 appeared highly effective within a very short time span, Cyclocybe aegerita Ser1 failed to degrade lignin. This situation was supported by very high enzyme activities and the low presence of phenolics in the media of Pleurotus ostreatus Ser1, compared to very low enzyme activity and the high presence of phenolics in the media with Cyclocybe aegerita Ser1.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
T1  - Functional differentiation of two autochthonous cohabiting strains of Pleurotus ostreatus and Cyclocybe aegerita from Serbia in lignin compound degradation
IS  - 1
SP  - 135–143
VL  - 47
DO  - 10.2298/BOTSERB2301135S
ER  - 

@article{
author = "Stefanović, Slobodan and Dragišić Maksimović, Jelena and Maksimović, Vuk and Bartolić, Dragana and Đikanović, Daniela and Simonović Radosavljević, Jasna and Mutavdžić, Dragosav and Radotić, Ksenija and Marjanović, Žaklina",
year = "2023",
abstract = "In nature, woody substrates are usually degraded by entire communities of microorganisms, which are nowadays jeopardised by anthropogenic influence, making it important to define the functional specificity of every species. Two strains of autochthonous fungi from Serbian lowland forests (Pleurotus ostreatus Ser1 and Cyclocybe aegerita Ser1) have been investigated for their ability to degrade lignin substrates [oak sawdust, oak isolated cell walls, and synthetic dehydrogenative polymer (DHP)]. Measuring the activities of the enzymes involved in lignin degradation was coupled with detecting the HPLC profile of the phenolics in the fungal growth media, and the lignin loss. While Pleurotus ostreatus Ser1 appeared highly effective within a very short time span, Cyclocybe aegerita Ser1 failed to degrade lignin. This situation was supported by very high enzyme activities and the low presence of phenolics in the media of Pleurotus ostreatus Ser1, compared to very low enzyme activity and the high presence of phenolics in the media with Cyclocybe aegerita Ser1.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
title = "Functional differentiation of two autochthonous cohabiting strains of Pleurotus ostreatus and Cyclocybe aegerita from Serbia in lignin compound degradation",
number = "1",
pages = "135–143",
volume = "47",
doi = "10.2298/BOTSERB2301135S"
}

Stefanović, S., Dragišić Maksimović, J., Maksimović, V., Bartolić, D., Đikanović, D., Simonović Radosavljević, J., Mutavdžić, D., Radotić, K.,& Marjanović, Ž.. (2023). Functional differentiation of two autochthonous cohabiting strains of Pleurotus ostreatus and Cyclocybe aegerita from Serbia in lignin compound degradation. 
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 47(1), 135–143.
https://doi.org/10.2298/BOTSERB2301135S

Stefanović S, Dragišić Maksimović J, Maksimović V, Bartolić D, Đikanović D, Simonović Radosavljević J, Mutavdžić D, Radotić K, Marjanović Ž. Functional differentiation of two autochthonous cohabiting strains of Pleurotus ostreatus and Cyclocybe aegerita from Serbia in lignin compound degradation. 2023;47(1):135–143.
doi:10.2298/BOTSERB2301135S .

Stefanović, Slobodan, Dragišić Maksimović, Jelena, Maksimović, Vuk, Bartolić, Dragana, Đikanović, Daniela, Simonović Radosavljević, Jasna, Mutavdžić, Dragosav, Radotić, Ksenija, Marjanović, Žaklina, "Functional differentiation of two autochthonous cohabiting strains of Pleurotus ostreatus and Cyclocybe aegerita from Serbia in lignin compound degradation", 47, no. 1 (2023):135–143,
https://doi.org/10.2298/BOTSERB2301135S . .

TY  - CONF
AU  - Kukobat, Lana
AU  - Marjanović, Mila
AU  - Drenovak Ivanović, Mirjana
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3000
AB  - General Laws on nature protection of Serbia (competent Ministry for Nature Protection) passively regulate fungi by regulating protection of the environment, biodiversity, diversity of ecosystems or landscapes in the country, in a way that should meet the requirements of the EU legislation system. The direct regulation of issues related to truffles is found in Rulebook on the declaration and protection of strictly protected and protected wild species of plants, animals and mushrooms and Decision on the determination of goods for which import, export, or transit requires the obtainment of certain documents. The truffle species included in these two documents are Tuber magnatum, Tuber aestivum and Tuber macrosporum. The Law on Forests, issued by the Ministry of Agriculture and Forestry directly treats mushrooms in two articles, one forbids collection of any secondary forest products, unless stated differently by that same Law, while other allows collection of secondary forest products only upon approval of the forest manager. It is not stated how these two articles were correlated with Rulebook and Decision of Law on nature Protection that refer to wild mushrooms including truffles. Apart from legal regulation in the commercial sense, there is absolutely no in purpose legal protection of the areas and ecosystems where truffles naturally occur. Without adequate protection of forest ecosystems that contain truffle species, there is a risk of losing these commercially important species. Establishment of truffle plantations is not regulated by any legal act, why the amendment of existing legislation is necessery in order to enable the developement of the entire truffles sector in the country.
PB  - University of Belgrade - Institutute for Multidisciplinary Research
C3  - Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
T1  - Legal regulation of truffle sector in the Republic of Serbia
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3000
ER  - 

@conference{
author = "Kukobat, Lana and Marjanović, Mila and Drenovak Ivanović, Mirjana and Marjanović, Žaklina",
year = "2023",
abstract = "General Laws on nature protection of Serbia (competent Ministry for Nature Protection) passively regulate fungi by regulating protection of the environment, biodiversity, diversity of ecosystems or landscapes in the country, in a way that should meet the requirements of the EU legislation system. The direct regulation of issues related to truffles is found in Rulebook on the declaration and protection of strictly protected and protected wild species of plants, animals and mushrooms and Decision on the determination of goods for which import, export, or transit requires the obtainment of certain documents. The truffle species included in these two documents are Tuber magnatum, Tuber aestivum and Tuber macrosporum. The Law on Forests, issued by the Ministry of Agriculture and Forestry directly treats mushrooms in two articles, one forbids collection of any secondary forest products, unless stated differently by that same Law, while other allows collection of secondary forest products only upon approval of the forest manager. It is not stated how these two articles were correlated with Rulebook and Decision of Law on nature Protection that refer to wild mushrooms including truffles. Apart from legal regulation in the commercial sense, there is absolutely no in purpose legal protection of the areas and ecosystems where truffles naturally occur. Without adequate protection of forest ecosystems that contain truffle species, there is a risk of losing these commercially important species. Establishment of truffle plantations is not regulated by any legal act, why the amendment of existing legislation is necessery in order to enable the developement of the entire truffles sector in the country.",
publisher = "University of Belgrade - Institutute for Multidisciplinary Research",
journal = "Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts",
title = "Legal regulation of truffle sector in the Republic of Serbia",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3000"
}

Kukobat, L., Marjanović, M., Drenovak Ivanović, M.,& Marjanović, Ž.. (2023). Legal regulation of truffle sector in the Republic of Serbia. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
University of Belgrade - Institutute for Multidisciplinary Research., 39.
https://hdl.handle.net/21.15107/rcub_rimsi_3000

Kukobat L, Marjanović M, Drenovak Ivanović M, Marjanović Ž. Legal regulation of truffle sector in the Republic of Serbia. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts. 2023;:39.
https://hdl.handle.net/21.15107/rcub_rimsi_3000 .

Kukobat, Lana, Marjanović, Mila, Drenovak Ivanović, Mirjana, Marjanović, Žaklina, "Legal regulation of truffle sector in the Republic of Serbia" in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts (2023):39,
https://hdl.handle.net/21.15107/rcub_rimsi_3000 .

TY  - CONF
AU  - Marjanović, Žaklina
AU  - Manojlović, Dragan
AU  - Saljnikov, Elmira
AU  - Stefanović, Slobodan
AU  - Nawaz, Ali
AU  - Wubet, Tesfay
AU  - Bragato, Gilberto
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3008
AB  - Lowlands of Central Serbia are mostly covered by alluvial soils, while the natural vegetation types are various alluvial forests differentiated by the distance from the river bad (dynamics of soil water potential regimes) and gradients of  soil structure, nutrient availability and soil pH (Marjanovic et al 2020). These forests have been heavily exploited for timber throughout the human history, but what has remained still represent significant hotspots of above and belowground biodiversity. The rare specificity of these ecosystems is the high diversity of truffles, fungi producing belowground fruiting bodies, among which the most prised white Piedmont truffle holds a special attention (Bragato and Marjanovic, 2016). 
In order to describe these special habitats of Balkan Peninsula, we have conducted various experiments, mostly concentrated on soils that support the fructification of this ecologically and economically important species. In this contribution we will summarize results on soil structure, soil water content dynamics, nutrient availability, seasonality of soil processes as well as seasonality of root associated mycobiomes of selected alluvial forests. We focus on connecting the fructification of white truffle to different soil factors (Marjanovic et al 2015), as well as on defining the differentiation factors that have been shaping root associated mycobiomes (Marjanovic et al 2020). The contribution was set to uncover as many soil traits as possible to reveal the answer to the question of what are the ideal conditions of soil/vegetation /climate combination that is suitable for such specific fungal communities.
PB  - https://wcss.speak.co.uk/
C3  - 22nd World Congress of Soil Science, Glasgow, 31-07.-05.08. 2022, Book of Abstracts
T1  - Environmental and biological specificities of the lowland alluvial soils in Central Serbia
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3008
ER  - 

@conference{
author = "Marjanović, Žaklina and Manojlović, Dragan and Saljnikov, Elmira and Stefanović, Slobodan and Nawaz, Ali and Wubet, Tesfay and Bragato, Gilberto",
year = "2023",
abstract = "Lowlands of Central Serbia are mostly covered by alluvial soils, while the natural vegetation types are various alluvial forests differentiated by the distance from the river bad (dynamics of soil water potential regimes) and gradients of  soil structure, nutrient availability and soil pH (Marjanovic et al 2020). These forests have been heavily exploited for timber throughout the human history, but what has remained still represent significant hotspots of above and belowground biodiversity. The rare specificity of these ecosystems is the high diversity of truffles, fungi producing belowground fruiting bodies, among which the most prised white Piedmont truffle holds a special attention (Bragato and Marjanovic, 2016). 
In order to describe these special habitats of Balkan Peninsula, we have conducted various experiments, mostly concentrated on soils that support the fructification of this ecologically and economically important species. In this contribution we will summarize results on soil structure, soil water content dynamics, nutrient availability, seasonality of soil processes as well as seasonality of root associated mycobiomes of selected alluvial forests. We focus on connecting the fructification of white truffle to different soil factors (Marjanovic et al 2015), as well as on defining the differentiation factors that have been shaping root associated mycobiomes (Marjanovic et al 2020). The contribution was set to uncover as many soil traits as possible to reveal the answer to the question of what are the ideal conditions of soil/vegetation /climate combination that is suitable for such specific fungal communities.",
publisher = "https://wcss.speak.co.uk/",
journal = "22nd World Congress of Soil Science, Glasgow, 31-07.-05.08. 2022, Book of Abstracts",
title = "Environmental and biological specificities of the lowland alluvial soils in Central Serbia",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3008"
}

Marjanović, Ž., Manojlović, D., Saljnikov, E., Stefanović, S., Nawaz, A., Wubet, T.,& Bragato, G.. (2023). Environmental and biological specificities of the lowland alluvial soils in Central Serbia. in 22nd World Congress of Soil Science, Glasgow, 31-07.-05.08. 2022, Book of Abstracts
https://wcss.speak.co.uk/..
https://hdl.handle.net/21.15107/rcub_rimsi_3008

Marjanović Ž, Manojlović D, Saljnikov E, Stefanović S, Nawaz A, Wubet T, Bragato G. Environmental and biological specificities of the lowland alluvial soils in Central Serbia. in 22nd World Congress of Soil Science, Glasgow, 31-07.-05.08. 2022, Book of Abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_rimsi_3008 .

Marjanović, Žaklina, Manojlović, Dragan, Saljnikov, Elmira, Stefanović, Slobodan, Nawaz, Ali, Wubet, Tesfay, Bragato, Gilberto, "Environmental and biological specificities of the lowland alluvial soils in Central Serbia" in 22nd World Congress of Soil Science, Glasgow, 31-07.-05.08. 2022, Book of Abstracts (2023),
https://hdl.handle.net/21.15107/rcub_rimsi_3008 .

TY  - CONF
AU  - Marjanović, Žaklina
AU  - Glišić, Aleksa
AU  - Stefanović, Slobodan
AU  - Glamočlija, Jasmina
AU  - Janošević, Dušica
AU  - Saljnikov, Elmira
AU  - Leonardi, Marco
AU  - Paccioni, Giovanni
AU  - Iotti, Mirco
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2972
AB  - Truffle diversity of Balkan Peninsula does not stop to surprise the scientific community with three new species from the melanosporum clade described in last year's (Mereny et al 2017, Milenković at al 2015,  Slavova et al 2023). Out of large set of ITS sequences,  Mereny at al. (2017) described T. cryptobrumale as connected to Pannonian region, but two other genetically quite distinctive populations were not distinguished as species. In this contribution, we have analyzed larger set of sequences and their habitats and detected strong connection between specimens with specific ITS/LSU region variants to ecologically specific habitats of Balkan peninsula. Soils and vegetation where these specimens have been detected rather resemble descriptions of T. melanosporum sites in Italy, while habitats of T. cryptobrumale resemble those of T. magnatum. Additionally, we have detected numerous populations of T. melanosporum in the coastal regions of Croatia, while this species has never been officially detected eastern from Dinaric alps in Balkan peninsula. We hypothesize that T. melanosporum could never overcome low temperatures of high and long Dinaric massive and is therefore localized in the western Mediterranean regions. This could be the reason why the melanosporum clade has been highly diversifying in Balkan regions. Moreover, the genetic variant widely distributed all over Europe and described as “T. brumale” has never been detected in the Balkans. Therefore, we propose that ecologically and genetically specific taxon that is widespread only in Balkan peninsula (and to some extent northwards) should be recognized as a different species - Tuber balkanicum.
PB  - University of Belgrade - Institutute for Multidisciplinary Research
C3  - Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
T1  - Tuber balkanicum – new taxon defined by molecular, ecological and biogeographical methods
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2972
ER  - 

@conference{
author = "Marjanović, Žaklina and Glišić, Aleksa and Stefanović, Slobodan and Glamočlija, Jasmina and Janošević, Dušica and Saljnikov, Elmira and Leonardi, Marco and Paccioni, Giovanni and Iotti, Mirco",
year = "2023",
abstract = "Truffle diversity of Balkan Peninsula does not stop to surprise the scientific community with three new species from the melanosporum clade described in last year's (Mereny et al 2017, Milenković at al 2015,  Slavova et al 2023). Out of large set of ITS sequences,  Mereny at al. (2017) described T. cryptobrumale as connected to Pannonian region, but two other genetically quite distinctive populations were not distinguished as species. In this contribution, we have analyzed larger set of sequences and their habitats and detected strong connection between specimens with specific ITS/LSU region variants to ecologically specific habitats of Balkan peninsula. Soils and vegetation where these specimens have been detected rather resemble descriptions of T. melanosporum sites in Italy, while habitats of T. cryptobrumale resemble those of T. magnatum. Additionally, we have detected numerous populations of T. melanosporum in the coastal regions of Croatia, while this species has never been officially detected eastern from Dinaric alps in Balkan peninsula. We hypothesize that T. melanosporum could never overcome low temperatures of high and long Dinaric massive and is therefore localized in the western Mediterranean regions. This could be the reason why the melanosporum clade has been highly diversifying in Balkan regions. Moreover, the genetic variant widely distributed all over Europe and described as “T. brumale” has never been detected in the Balkans. Therefore, we propose that ecologically and genetically specific taxon that is widespread only in Balkan peninsula (and to some extent northwards) should be recognized as a different species - Tuber balkanicum.",
publisher = "University of Belgrade - Institutute for Multidisciplinary Research",
journal = "Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts",
title = "Tuber balkanicum – new taxon defined by molecular, ecological and biogeographical methods",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2972"
}

Marjanović, Ž., Glišić, A., Stefanović, S., Glamočlija, J., Janošević, D., Saljnikov, E., Leonardi, M., Paccioni, G.,& Iotti, M.. (2023). Tuber balkanicum – new taxon defined by molecular, ecological and biogeographical methods. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
University of Belgrade - Institutute for Multidisciplinary Research., 19.
https://hdl.handle.net/21.15107/rcub_rimsi_2972

Marjanović Ž, Glišić A, Stefanović S, Glamočlija J, Janošević D, Saljnikov E, Leonardi M, Paccioni G, Iotti M. Tuber balkanicum – new taxon defined by molecular, ecological and biogeographical methods. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts. 2023;:19.
https://hdl.handle.net/21.15107/rcub_rimsi_2972 .

Marjanović, Žaklina, Glišić, Aleksa, Stefanović, Slobodan, Glamočlija, Jasmina, Janošević, Dušica, Saljnikov, Elmira, Leonardi, Marco, Paccioni, Giovanni, Iotti, Mirco, "Tuber balkanicum – new taxon defined by molecular, ecological and biogeographical methods" in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts (2023):19,
https://hdl.handle.net/21.15107/rcub_rimsi_2972 .

TY  - CONF
AU  - Bragato, Gilberto
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2951
AB  - Knowledge about the environments of T. magnatum has been consolidated in the 2000s, confirming the importance of microclimate and that of specific soil types that, in fact, restrict the areas of natural spread of the fungus. In recent years, investigations have focused on two distinct themes: on the one hand, mycorrhization techniques have been refined, leading to the commercial production of mycorrhized plants and the establishment of the first experimental truffle orchards; on the other hand, the hydrological aspects of natural truffle areas, previously left in the background, have begun to be studied. The first topic has yielded interesting results with the start of production in areas never reported as productive and promises to expand the territories in which to "cultivate" T. magnatum. The second line of investigation is providing insights into the need for specific water management of truffles - natural and cultivated - focused on soil water conservation. For the future, it will be necessary to work in depth on this aspect. The natural environments of T. magnatum are characterized by a great need for water, which we hypothesize can be managed at the scale of micro-hydrographic basin, since the exclusive use of irrigation practices adopted, for example, for T. melanosporum is not economically viable.
PB  - University of Belgrade - Institutute for Multidisciplinary Research
C3  - Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
T1  - Where do we stand on the knowledge of T. magnatum environments? Progress and new research hypotheses
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2951
ER  - 

@conference{
author = "Bragato, Gilberto and Marjanović, Žaklina",
year = "2023",
abstract = "Knowledge about the environments of T. magnatum has been consolidated in the 2000s, confirming the importance of microclimate and that of specific soil types that, in fact, restrict the areas of natural spread of the fungus. In recent years, investigations have focused on two distinct themes: on the one hand, mycorrhization techniques have been refined, leading to the commercial production of mycorrhized plants and the establishment of the first experimental truffle orchards; on the other hand, the hydrological aspects of natural truffle areas, previously left in the background, have begun to be studied. The first topic has yielded interesting results with the start of production in areas never reported as productive and promises to expand the territories in which to "cultivate" T. magnatum. The second line of investigation is providing insights into the need for specific water management of truffles - natural and cultivated - focused on soil water conservation. For the future, it will be necessary to work in depth on this aspect. The natural environments of T. magnatum are characterized by a great need for water, which we hypothesize can be managed at the scale of micro-hydrographic basin, since the exclusive use of irrigation practices adopted, for example, for T. melanosporum is not economically viable.",
publisher = "University of Belgrade - Institutute for Multidisciplinary Research",
journal = "Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts",
title = "Where do we stand on the knowledge of T. magnatum environments? Progress and new research hypotheses",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2951"
}

Bragato, G.,& Marjanović, Ž.. (2023). Where do we stand on the knowledge of T. magnatum environments? Progress and new research hypotheses. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
University of Belgrade - Institutute for Multidisciplinary Research., 18.
https://hdl.handle.net/21.15107/rcub_rimsi_2951

Bragato G, Marjanović Ž. Where do we stand on the knowledge of T. magnatum environments? Progress and new research hypotheses. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts. 2023;:18.
https://hdl.handle.net/21.15107/rcub_rimsi_2951 .

Bragato, Gilberto, Marjanović, Žaklina, "Where do we stand on the knowledge of T. magnatum environments? Progress and new research hypotheses" in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts (2023):18,
https://hdl.handle.net/21.15107/rcub_rimsi_2951 .

TY  - CONF
AU  - Belfiori, Beatrice
AU  - Riccioni, Claudia
AU  - Gómez-Molina, Eva
AU  - Sánchez, Sergio
AU  - Gallo, Luigi
AU  - García-Barreda, Sergi
AU  - Henkrar, Fatima
AU  - Khabar, Lahsen
AU  - Marjanović, Žaklina
AU  - Rubini, Andrea
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2907
AB  - Tuber aestivum is associated with a wide range of tree species and is the most widespread edible truffle. Its capability of colonizing many different areas along with a long reaping period, increased in the last decades scientific studies and commercial interest.
A number of large- and fine-scale population genetics studies have been conducted to explore its genetic diversity and genetic structure.
Recent investigations based on ITS sequence analyses revealed a strong phylogenetic pattern and a higher level of genetic diversity in Turkish and southern European populations than in northern European populations, suggesting that Turkey and southern Europe may have acted as glacial refugia for this species. 
Despite these findings, the southernmost distributional range of the species is not yet fully explored. With the aim of evaluating other potential diversity hotspots in this study we expand previous investigation, extending the sampling to other southernmost natural T. aestivum areas, such as Moroccan mountainous regions (Middle Atlas), Serbia and several Spanish regions. 
The ongoing analyses based on haplotypes alignment of the ITS region of the rDNA, reveal as preliminary result a pronounced genetic variability among Moroccan T. aestivum populations, suggesting a possible phylogeographic differentiation also in these new areas of investigation. Additional analyses are underway to better trace natural populations of T. aestivum according to their geographic origin.
These aims could be critical both for truffle industry interest in selecting well-adapted inoculum for the production of mycorrhizal seedlings or traceability of truffles provenance, and for the establishment of programs devoted to biodiversity conservation.
PB  - University of Belgrade - Institutute for Multidisciplinary Research
C3  - Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
T1  - Exploring new biodiversity hotspots in Tuber aestivum  natural distribution
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2907
ER  - 

@conference{
author = "Belfiori, Beatrice and Riccioni, Claudia and Gómez-Molina, Eva and Sánchez, Sergio and Gallo, Luigi and García-Barreda, Sergi and Henkrar, Fatima and Khabar, Lahsen and Marjanović, Žaklina and Rubini, Andrea",
year = "2023",
abstract = "Tuber aestivum is associated with a wide range of tree species and is the most widespread edible truffle. Its capability of colonizing many different areas along with a long reaping period, increased in the last decades scientific studies and commercial interest.
A number of large- and fine-scale population genetics studies have been conducted to explore its genetic diversity and genetic structure.
Recent investigations based on ITS sequence analyses revealed a strong phylogenetic pattern and a higher level of genetic diversity in Turkish and southern European populations than in northern European populations, suggesting that Turkey and southern Europe may have acted as glacial refugia for this species. 
Despite these findings, the southernmost distributional range of the species is not yet fully explored. With the aim of evaluating other potential diversity hotspots in this study we expand previous investigation, extending the sampling to other southernmost natural T. aestivum areas, such as Moroccan mountainous regions (Middle Atlas), Serbia and several Spanish regions. 
The ongoing analyses based on haplotypes alignment of the ITS region of the rDNA, reveal as preliminary result a pronounced genetic variability among Moroccan T. aestivum populations, suggesting a possible phylogeographic differentiation also in these new areas of investigation. Additional analyses are underway to better trace natural populations of T. aestivum according to their geographic origin.
These aims could be critical both for truffle industry interest in selecting well-adapted inoculum for the production of mycorrhizal seedlings or traceability of truffles provenance, and for the establishment of programs devoted to biodiversity conservation.",
publisher = "University of Belgrade - Institutute for Multidisciplinary Research",
journal = "Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts",
title = "Exploring new biodiversity hotspots in Tuber aestivum  natural distribution",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2907"
}

Belfiori, B., Riccioni, C., Gómez-Molina, E., Sánchez, S., Gallo, L., García-Barreda, S., Henkrar, F., Khabar, L., Marjanović, Ž.,& Rubini, A.. (2023). Exploring new biodiversity hotspots in Tuber aestivum  natural distribution. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
University of Belgrade - Institutute for Multidisciplinary Research., 33.
https://hdl.handle.net/21.15107/rcub_rimsi_2907

Belfiori B, Riccioni C, Gómez-Molina E, Sánchez S, Gallo L, García-Barreda S, Henkrar F, Khabar L, Marjanović Ž, Rubini A. Exploring new biodiversity hotspots in Tuber aestivum  natural distribution. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts. 2023;:33.
https://hdl.handle.net/21.15107/rcub_rimsi_2907 .

Belfiori, Beatrice, Riccioni, Claudia, Gómez-Molina, Eva, Sánchez, Sergio, Gallo, Luigi, García-Barreda, Sergi, Henkrar, Fatima, Khabar, Lahsen, Marjanović, Žaklina, Rubini, Andrea, "Exploring new biodiversity hotspots in Tuber aestivum  natural distribution" in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts (2023):33,
https://hdl.handle.net/21.15107/rcub_rimsi_2907 .

TY  - CONF
AU  - Grujić, Tara
AU  - Saljnikov, Elmira
AU  - Stefanović, Slobodan
AU  - Belanović Simić, Snežana
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2930
AB  - The impact of plastic pollution on forest soil biota is an emerging research area. There are knowledge gaps on a forest microbiome composition and activity as effected by wild waste deposition in forest ecosystems located on white truffle (Tuber magnatum Pico) habitat areas. Particles of plastic materials decompose into micro-plastic particles and are responsible for many changes in the soil physicochemical characteristics, including porosity, enzymatic activities, microbial activities, plant growth. One of the first investigations of plastic and microplastic (MP) in the soil on the territory of Serbia is currently underway and is being carried out within the project "Evaluation of the Microplastic in the Soils of Serbia - EMIPLAST - SoS". The research sites are located on the alluvial plains of the largest rivers in Serbia, which are the habitats of the white truffle.
Forest sites visibly polluted and non-polluted by plastic materials were sampled and analysed for soil main chemical, physical and biological properties. Potentially mineralizable soil organic carbon was measured in a long-term incubation of soil samples with sequential measurement of the emitted CO2 flax by alkali trap method. 
Preliminary results showed that some soil properties such as pH, electrical conductivity, total C and N, CEC and mechanical composition are significantly affected by the presence of plastic materials. Generally, polluted sites showed elevated microbial respiration and the rates of C mineralization. For the Danube and Morava River basin the differences were significant, while for the Sava river basin the difference wasn’t significant. In order to establish the level of the negative impact of microplastics on soil properties and microbial activity in the longer term, the study is ongoing.
PB  - University of Belgrade - Institutute for Multidisciplinary Research
C3  - Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
T1  - Impact of plastic pollution on soil biological activity in white truffle habitats in the large river plains in Serbia
SP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2930
ER  - 

@conference{
author = "Grujić, Tara and Saljnikov, Elmira and Stefanović, Slobodan and Belanović Simić, Snežana and Marjanović, Žaklina",
year = "2023",
abstract = "The impact of plastic pollution on forest soil biota is an emerging research area. There are knowledge gaps on a forest microbiome composition and activity as effected by wild waste deposition in forest ecosystems located on white truffle (Tuber magnatum Pico) habitat areas. Particles of plastic materials decompose into micro-plastic particles and are responsible for many changes in the soil physicochemical characteristics, including porosity, enzymatic activities, microbial activities, plant growth. One of the first investigations of plastic and microplastic (MP) in the soil on the territory of Serbia is currently underway and is being carried out within the project "Evaluation of the Microplastic in the Soils of Serbia - EMIPLAST - SoS". The research sites are located on the alluvial plains of the largest rivers in Serbia, which are the habitats of the white truffle.
Forest sites visibly polluted and non-polluted by plastic materials were sampled and analysed for soil main chemical, physical and biological properties. Potentially mineralizable soil organic carbon was measured in a long-term incubation of soil samples with sequential measurement of the emitted CO2 flax by alkali trap method. 
Preliminary results showed that some soil properties such as pH, electrical conductivity, total C and N, CEC and mechanical composition are significantly affected by the presence of plastic materials. Generally, polluted sites showed elevated microbial respiration and the rates of C mineralization. For the Danube and Morava River basin the differences were significant, while for the Sava river basin the difference wasn’t significant. In order to establish the level of the negative impact of microplastics on soil properties and microbial activity in the longer term, the study is ongoing.",
publisher = "University of Belgrade - Institutute for Multidisciplinary Research",
journal = "Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts",
title = "Impact of plastic pollution on soil biological activity in white truffle habitats in the large river plains in Serbia",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2930"
}

Grujić, T., Saljnikov, E., Stefanović, S., Belanović Simić, S.,& Marjanović, Ž.. (2023). Impact of plastic pollution on soil biological activity in white truffle habitats in the large river plains in Serbia. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts
University of Belgrade - Institutute for Multidisciplinary Research., 20.
https://hdl.handle.net/21.15107/rcub_rimsi_2930

Grujić T, Saljnikov E, Stefanović S, Belanović Simić S, Marjanović Ž. Impact of plastic pollution on soil biological activity in white truffle habitats in the large river plains in Serbia. in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts. 2023;:20.
https://hdl.handle.net/21.15107/rcub_rimsi_2930 .

Grujić, Tara, Saljnikov, Elmira, Stefanović, Slobodan, Belanović Simić, Snežana, Marjanović, Žaklina, "Impact of plastic pollution on soil biological activity in white truffle habitats in the large river plains in Serbia" in Truffle Research Union of Europe Conference, September 27-30., Belgrade, Serbia, Book of Abstracts (2023):20,
https://hdl.handle.net/21.15107/rcub_rimsi_2930 .

TY  - CONF
AU  - Grujić, Tara
AU  - Saljnikov, Elmira
AU  - Jovković, Marina
AU  - Belanovic Simic, Snezana
AU  - Krnjajić, Slobodan
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2731
AB  - Plastic pollution is fast becoming a serious global environmental problem with the increase in plastic waste over recent decades. One of the first investigations of plastic and microplastic (MP) in the soil on the territory  of Serbia is currently underway and is being carried out within the project "Evaluation of the microplastic in the Soils of Serbia – EMIPLAST – SoS" funded by the Science Fund of the Republic of Serbia. The aim of the research is to reveal the impact of the presence of plastic materials on soil’s main chemical, physical and biological properties. The examination of the impact of MP on the soil is being carried out through a comparative analysis of samples from localities that are and are not exposed to MP pollution. Sampling was done in three seasonal repetitions in the 2022 and will be done in the same way in 2023 in all selected plots. Microbial respiration is measured from all samples using the alkaline trap method as an indicator of microbial activity. The parameters related to the soil structure are not variable in such a short time frame, which is why they were determined at the beginning and will be determined at the end of the experimental period: mechanical composition, volumetric mass, specific mass, porosity, aggregate stability and organic matter content. Soil parameters that may affect aggregate stability such as pH, electrical conductivity and total carbon content, as well as soil nutritional status (N, P2O5, K2O, Cu, Zn, Mn and CaCO3) were determined.
These parameters are used to characterize the soil at the research sites. Preliminary results showed that some soil properties are significantly affected by the presence of plastic materials. In order to establish the level of the negative impact of microplastics on soil properties and microbial activity in the longer term, the study is ongoing.
PB  - University of Belgrade, Faculty of Geography
PB  - University of Banja Luka, Faculty of Natural Sciences and Mathematics
PB  - Serbian Academy of Sciences and Arts, Geographical Institute “Jovan Cvijić”, Serbia
PB  - Faculty of Geography, Lomonosov Moscow State University
PB  - Russian Geographical Society
PB  - The World Association of Soil and Water Conservation (WASWAC) a
C3  - International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS
T1  - ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT
EP  - 39
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2731
ER  - 

@conference{
author = "Grujić, Tara and Saljnikov, Elmira and Jovković, Marina and Belanovic Simic, Snezana and Krnjajić, Slobodan and Marjanović, Žaklina",
year = "2023",
abstract = "Plastic pollution is fast becoming a serious global environmental problem with the increase in plastic waste over recent decades. One of the first investigations of plastic and microplastic (MP) in the soil on the territory  of Serbia is currently underway and is being carried out within the project "Evaluation of the microplastic in the Soils of Serbia – EMIPLAST – SoS" funded by the Science Fund of the Republic of Serbia. The aim of the research is to reveal the impact of the presence of plastic materials on soil’s main chemical, physical and biological properties. The examination of the impact of MP on the soil is being carried out through a comparative analysis of samples from localities that are and are not exposed to MP pollution. Sampling was done in three seasonal repetitions in the 2022 and will be done in the same way in 2023 in all selected plots. Microbial respiration is measured from all samples using the alkaline trap method as an indicator of microbial activity. The parameters related to the soil structure are not variable in such a short time frame, which is why they were determined at the beginning and will be determined at the end of the experimental period: mechanical composition, volumetric mass, specific mass, porosity, aggregate stability and organic matter content. Soil parameters that may affect aggregate stability such as pH, electrical conductivity and total carbon content, as well as soil nutritional status (N, P2O5, K2O, Cu, Zn, Mn and CaCO3) were determined.
These parameters are used to characterize the soil at the research sites. Preliminary results showed that some soil properties are significantly affected by the presence of plastic materials. In order to establish the level of the negative impact of microplastics on soil properties and microbial activity in the longer term, the study is ongoing.",
publisher = "University of Belgrade, Faculty of Geography, University of Banja Luka, Faculty of Natural Sciences and Mathematics, Serbian Academy of Sciences and Arts, Geographical Institute “Jovan Cvijić”, Serbia, Faculty of Geography, Lomonosov Moscow State University, Russian Geographical Society, The World Association of Soil and Water Conservation (WASWAC) a",
journal = "International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS",
title = "ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT",
pages = "39-39",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2731"
}

Grujić, T., Saljnikov, E., Jovković, M., Belanovic Simic, S., Krnjajić, S.,& Marjanović, Ž.. (2023). ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT. in International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS
University of Belgrade, Faculty of Geography., 39-39.
https://hdl.handle.net/21.15107/rcub_rimsi_2731

Grujić T, Saljnikov E, Jovković M, Belanovic Simic S, Krnjajić S, Marjanović Ž. ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT. in International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS. 2023;:39-39.
https://hdl.handle.net/21.15107/rcub_rimsi_2731 .

Grujić, Tara, Saljnikov, Elmira, Jovković, Marina, Belanovic Simic, Snezana, Krnjajić, Slobodan, Marjanović, Žaklina, "ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT" in International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS (2023):39-39,
https://hdl.handle.net/21.15107/rcub_rimsi_2731 .

TY  - CONF
AU  - Krnjajić, Slobodan
AU  - Štrbac, Filip
AU  - Radović, Vesela
AU  - Stanković, Slađan
AU  - Tomić, Vedran
AU  - Stefanović, Slobodan
AU  - Knežević, Goran
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2435
AB  - Plastic has been recognized as one of the leading pollutants of all ecosystems. This question has been rarely raised in Serbia, although there is a significant problem with the disposal of communal and agricultural waste. Many wild landfills have been created in past decades in forests near the plasticulture practicing areas and rivers. Plastic materials are degraded by the influence of UV light and various environmental factors, creating smaller particles called microplastics, which are recognized as significantly dangerous to ecosystems. Alluvial planes have been the most endangered due to the horizontal and vertical fluvial activities of the nearby rivers. The influence of soil microplastics on insect communities has not been widely considered and that is why the project EMIPLAST-SoS has been created to investigate insect communities in polluted and unpolluted alluvial soils in Serbia. We compare them in forest and agricultural ecosystems on similar alluvial soils in three locations near three largest rivers in Serbia. Preliminary results from one-season sampling are considered. During the project the influence of the different seasons effects will be investigated. Additionally, we have investigated the rate of plastic decomposition by five insect species in lab conditions (Plodia interpunctella Hubner, Tenebrio molitor L., Zophobas morio Fabr., Achroia grisella Fabr. and Galleria mellonela L.). G. mellonela expressed the highest and the most diverse decomposition of different plastic sources like plastic bags, sponges and styrofoam. The analyses of the obtained plastic residues are still underway.
PB  - Hellenic Entomological Society
C3  - Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece
T1  - The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects
EP  - 422
SP  - 421
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2435
ER  - 

@conference{
author = "Krnjajić, Slobodan and Štrbac, Filip and Radović, Vesela and Stanković, Slađan and Tomić, Vedran and Stefanović, Slobodan and Knežević, Goran and Marjanović, Žaklina",
year = "2023",
abstract = "Plastic has been recognized as one of the leading pollutants of all ecosystems. This question has been rarely raised in Serbia, although there is a significant problem with the disposal of communal and agricultural waste. Many wild landfills have been created in past decades in forests near the plasticulture practicing areas and rivers. Plastic materials are degraded by the influence of UV light and various environmental factors, creating smaller particles called microplastics, which are recognized as significantly dangerous to ecosystems. Alluvial planes have been the most endangered due to the horizontal and vertical fluvial activities of the nearby rivers. The influence of soil microplastics on insect communities has not been widely considered and that is why the project EMIPLAST-SoS has been created to investigate insect communities in polluted and unpolluted alluvial soils in Serbia. We compare them in forest and agricultural ecosystems on similar alluvial soils in three locations near three largest rivers in Serbia. Preliminary results from one-season sampling are considered. During the project the influence of the different seasons effects will be investigated. Additionally, we have investigated the rate of plastic decomposition by five insect species in lab conditions (Plodia interpunctella Hubner, Tenebrio molitor L., Zophobas morio Fabr., Achroia grisella Fabr. and Galleria mellonela L.). G. mellonela expressed the highest and the most diverse decomposition of different plastic sources like plastic bags, sponges and styrofoam. The analyses of the obtained plastic residues are still underway.",
publisher = "Hellenic Entomological Society",
journal = "Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece",
title = "The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects",
pages = "422-421",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2435"
}

Krnjajić, S., Štrbac, F., Radović, V., Stanković, S., Tomić, V., Stefanović, S., Knežević, G.,& Marjanović, Ž.. (2023). The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects. in Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece
Hellenic Entomological Society., 421-422.
https://hdl.handle.net/21.15107/rcub_rimsi_2435

Krnjajić S, Štrbac F, Radović V, Stanković S, Tomić V, Stefanović S, Knežević G, Marjanović Ž. The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects. in Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece. 2023;:421-422.
https://hdl.handle.net/21.15107/rcub_rimsi_2435 .

Krnjajić, Slobodan, Štrbac, Filip, Radović, Vesela, Stanković, Slađan, Tomić, Vedran, Stefanović, Slobodan, Knežević, Goran, Marjanović, Žaklina, "The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects" in Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece (2023):421-422,
https://hdl.handle.net/21.15107/rcub_rimsi_2435 .

TY  - CONF
AU  - Saljnikov, Elmira
AU  - Grujić, Tara
AU  - Jovković, Marina
AU  - Stanković, Slađan
AU  - Krnjajić, Slobodan
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2466
AB  - Plastic pollution is fast becoming a serious global environmental problem with the increase in plastic waste over recent decades. Plastic pollutants in the soil environment have an impact on the cycling of organic matter, global СО2 production, crop production, and soil properties. Crop production in plastic greenhouses is one of the serious sources of plastic pollution in the environment all over the world and particularly in Serbia. One of the
first investigations of plastic and microplastic materials in the soil on the territory of Serbia is currently underway and is being carried out within the project „Evaluation of the Microplastic in the Soils of Serbia – EMIPLAST – SoS“ funded by the Science Fund of the Republic of Serbia. The aim of the research was to reveal the impact of the presence of plastic materials on soil’s main chemical, physical and biological properties. Soil sampling was carried out in 2022 from two depths (0–15 cm and 15–30 cm), side by side from the plastic greenhouse and non-greenhouse agricultural production as a control in the first project year. The research sites are located on the alluvial plains of the two largest rivers in Serbia, the Danube and the Sava. The following chemical parameters were determined on all samples: electrical conductivity, pH, CEC, total C, as well as nutritional status of the soil (N, P2O5, K2O, Cu, Zn, Mn and CaCO3). Concentrations of some microelements were also analyzed. Physical parameters determined were: particle size distribution, volumetric mass, specific mass and porosity. Microbial respiration, which serves as an indicator of enzymatic microbial activity in the soil, was measured by the alkaline trap method from surface soil.
C3  - The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World”
T1  - Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia)
EP  - 59
SP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2466
ER  - 

@conference{
author = "Saljnikov, Elmira and Grujić, Tara and Jovković, Marina and Stanković, Slađan and Krnjajić, Slobodan and Marjanović, Žaklina",
year = "2023",
abstract = "Plastic pollution is fast becoming a serious global environmental problem with the increase in plastic waste over recent decades. Plastic pollutants in the soil environment have an impact on the cycling of organic matter, global СО2 production, crop production, and soil properties. Crop production in plastic greenhouses is one of the serious sources of plastic pollution in the environment all over the world and particularly in Serbia. One of the
first investigations of plastic and microplastic materials in the soil on the territory of Serbia is currently underway and is being carried out within the project „Evaluation of the Microplastic in the Soils of Serbia – EMIPLAST – SoS“ funded by the Science Fund of the Republic of Serbia. The aim of the research was to reveal the impact of the presence of plastic materials on soil’s main chemical, physical and biological properties. Soil sampling was carried out in 2022 from two depths (0–15 cm and 15–30 cm), side by side from the plastic greenhouse and non-greenhouse agricultural production as a control in the first project year. The research sites are located on the alluvial plains of the two largest rivers in Serbia, the Danube and the Sava. The following chemical parameters were determined on all samples: electrical conductivity, pH, CEC, total C, as well as nutritional status of the soil (N, P2O5, K2O, Cu, Zn, Mn and CaCO3). Concentrations of some microelements were also analyzed. Physical parameters determined were: particle size distribution, volumetric mass, specific mass and porosity. Microbial respiration, which serves as an indicator of enzymatic microbial activity in the soil, was measured by the alkaline trap method from surface soil.",
journal = "The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World”",
title = "Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia)",
pages = "59-58",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2466"
}

Saljnikov, E., Grujić, T., Jovković, M., Stanković, S., Krnjajić, S.,& Marjanović, Ž.. (2023). Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia). in The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World”, 58-59.
https://hdl.handle.net/21.15107/rcub_rimsi_2466

Saljnikov E, Grujić T, Jovković M, Stanković S, Krnjajić S, Marjanović Ž. Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia). in The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World”. 2023;:58-59.
https://hdl.handle.net/21.15107/rcub_rimsi_2466 .

Saljnikov, Elmira, Grujić, Tara, Jovković, Marina, Stanković, Slađan, Krnjajić, Slobodan, Marjanović, Žaklina, "Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia)" in The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World” (2023):58-59,
https://hdl.handle.net/21.15107/rcub_rimsi_2466 .

TY  - JOUR
AU  - Bragato, Gilberto
AU  - Fornasier, Flavio
AU  - Bagi, Istvan
AU  - Egli, Simon
AU  - Marjanović, Žaklina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1396
AB  - Truffles are ectomycorrhizal fungi of which several species can be commercially produced in man-made plan-tations using trees inoculated with their mycelia. The production in tree plantations is meant to increase the availability of economically valued ascocarps on the market and, in the same time preserve natural habitats from an excessive pressure of truffle hunters, as well as limit the damage that they may face due to the inadequate management of truffle-producing regions. Even though plantations of summer truffle (Tuber aestivum Vittad.) are the most ubiquitous in France and Italy, the Jaszsag region in Hungary has been recently recognized as one of the most productive areas in Europe. Forestry practice in this region often assumes the renovation of autochthonous tree stands by massive sowing of the tree seeds. One of these plantations of oaks (Quercus robur and Q. cerris), spontaneously colonized without artificial inoculation, appeared to produce the highest officially reported amounts of summer truffles in Europe. Therefore, the managing forestry company decided to apply practice that aimed at maintenance of high truffle production. Assuming that climatic and vegetation factors have no impact on ascocarp production within the stand, the plantation provided perfect experimental setup for investigating the influence of very localised soil properties and terrain morphology on truffle abundance. Therefore, in this contribution selected soil properties have been investigated in the entire plantation using specifically designed protocols to characterize the site and find out if the spatial variation in the truffle production can be explained by short-distance differences in soil properties. In the Chernozem soil type that dominated the entire forest stand, soil organic matter (SOM), soluble nitrogen (TN) and properties influenced by SOM and biological activity were positively correlated with high ascocarp production, whereas increased soil aggregate size and clay content was negatively correlated with productivity. The spatial distribution of these parameters appeared strongly related to ascocarp abundance distribution. Chernozem seems to be an ideal type of soil for Tuber aestivum ascocarp production, but terrain morphology and soil properties spatial pattern, which may have been historically influenced by fluvial and aeolian processes, can significantly influence the rate of production. In the case of non wood products such as truffles, forest management will necessarily have to consider the conditions of the soil environment that, influencing the presence of the truffle species, can make their production interesting in terms of income for the forest owner.
PB  - Elsevier, Amsterdam
T2  - Forest Ecology and Management
T1  - Soil parameters explain short-distance variation in production of Tuber aestivum Vittad. in an oak plantation in the central-northern part of the Great Hungarian Plain (Jaszsag region, Hungary)
VL  - 479
DO  - 10.1016/j.foreco.2020.118578
ER  - 

@article{
author = "Bragato, Gilberto and Fornasier, Flavio and Bagi, Istvan and Egli, Simon and Marjanović, Žaklina",
year = "2021",
abstract = "Truffles are ectomycorrhizal fungi of which several species can be commercially produced in man-made plan-tations using trees inoculated with their mycelia. The production in tree plantations is meant to increase the availability of economically valued ascocarps on the market and, in the same time preserve natural habitats from an excessive pressure of truffle hunters, as well as limit the damage that they may face due to the inadequate management of truffle-producing regions. Even though plantations of summer truffle (Tuber aestivum Vittad.) are the most ubiquitous in France and Italy, the Jaszsag region in Hungary has been recently recognized as one of the most productive areas in Europe. Forestry practice in this region often assumes the renovation of autochthonous tree stands by massive sowing of the tree seeds. One of these plantations of oaks (Quercus robur and Q. cerris), spontaneously colonized without artificial inoculation, appeared to produce the highest officially reported amounts of summer truffles in Europe. Therefore, the managing forestry company decided to apply practice that aimed at maintenance of high truffle production. Assuming that climatic and vegetation factors have no impact on ascocarp production within the stand, the plantation provided perfect experimental setup for investigating the influence of very localised soil properties and terrain morphology on truffle abundance. Therefore, in this contribution selected soil properties have been investigated in the entire plantation using specifically designed protocols to characterize the site and find out if the spatial variation in the truffle production can be explained by short-distance differences in soil properties. In the Chernozem soil type that dominated the entire forest stand, soil organic matter (SOM), soluble nitrogen (TN) and properties influenced by SOM and biological activity were positively correlated with high ascocarp production, whereas increased soil aggregate size and clay content was negatively correlated with productivity. The spatial distribution of these parameters appeared strongly related to ascocarp abundance distribution. Chernozem seems to be an ideal type of soil for Tuber aestivum ascocarp production, but terrain morphology and soil properties spatial pattern, which may have been historically influenced by fluvial and aeolian processes, can significantly influence the rate of production. In the case of non wood products such as truffles, forest management will necessarily have to consider the conditions of the soil environment that, influencing the presence of the truffle species, can make their production interesting in terms of income for the forest owner.",
publisher = "Elsevier, Amsterdam",
journal = "Forest Ecology and Management",
title = "Soil parameters explain short-distance variation in production of Tuber aestivum Vittad. in an oak plantation in the central-northern part of the Great Hungarian Plain (Jaszsag region, Hungary)",
volume = "479",
doi = "10.1016/j.foreco.2020.118578"
}

Bragato, G., Fornasier, F., Bagi, I., Egli, S.,& Marjanović, Ž.. (2021). Soil parameters explain short-distance variation in production of Tuber aestivum Vittad. in an oak plantation in the central-northern part of the Great Hungarian Plain (Jaszsag region, Hungary). in Forest Ecology and Management
Elsevier, Amsterdam., 479.
https://doi.org/10.1016/j.foreco.2020.118578

Bragato G, Fornasier F, Bagi I, Egli S, Marjanović Ž. Soil parameters explain short-distance variation in production of Tuber aestivum Vittad. in an oak plantation in the central-northern part of the Great Hungarian Plain (Jaszsag region, Hungary). in Forest Ecology and Management. 2021;479.
doi:10.1016/j.foreco.2020.118578 .

Bragato, Gilberto, Fornasier, Flavio, Bagi, Istvan, Egli, Simon, Marjanović, Žaklina, "Soil parameters explain short-distance variation in production of Tuber aestivum Vittad. in an oak plantation in the central-northern part of the Great Hungarian Plain (Jaszsag region, Hungary)" in Forest Ecology and Management, 479 (2021),
https://doi.org/10.1016/j.foreco.2020.118578 . .

TY  - JOUR
AU  - Marjanović, Žaklina
AU  - Nawaz, Ali
AU  - Stevanović, Katarina
AU  - Saljnikov, Elmira
AU  - Macek, Irena
AU  - Oehl, Fritz
AU  - Wubet, Tesfaye
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1370
AB  - Balkan lowlands bordering with the Pannonia region are inhabited by diverse riparian forests that support production of different truffle species, predominantly the most prized white truffle of Piedmont (Tuber magnatumPico), but also other commercial species (T.macrosporumVitt.,T.aestivumVitt.). Surprisingly, little is known about the native root-associated mycobiome (RAM) of these lowland truffle-producing forests. Therefore, in this study we aim at exploring and comparing the RAMs of three different truffle-producing forests from Kolubara river plane in Serbia. Molecular methods based on next generation sequencing (NGS) were used to evaluate the diversity of root-associated fungal communities and to elucidate the influence of environmental factors on their differentiation. To our knowledge, this is the first study from such habitats with a particular focus on comparative analysis of the RAM in different truffle-producing habitats using a high-throughput sequencing approach. Our results indicated that the alpha diversity of investigated fungal communities was not significantly different between different truffle-producing forests and within a specific forest type, while the seasonal differences in the alpha diversity were only observed in the white truffle-producing forests. Taxonomic profiling at phylum level indicated the dominance of fungal OTUs belonging to phylum Ascomycota and Basidiomycota, with very minor presence of other phyla. Distinct community structures of root-associated mycobiomes were observed for white, mixed, and black truffle-producing forests. The core mycobiome analysis indicated a fair share of fungal genera present exclusively in white and black truffle-producing forest, while the core genera of mixed truffle-producing forests were shared with both white and black truffle-producing forests. The majority of detected fungal OTUs in all three forest types were symbiotrophs, with ectomycorrhizal fungi being a dominant functional guild. Apart from assumed vegetation factor, differentiation of fungal communities was driven by factors connected to the distance from the river and exposure to fluvial activities, soil age, structure, and pH. Overall, Pannonian riparian forests appear to host diverse root-associated fungal communities that are strongly shaped by variation in soil conditions.
PB  - MDPI, Basel
T2  - Microorganisms
T1  - Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests
IS  - 9
VL  - 8
DO  - 10.3390/microorganisms8091331
ER  - 

@article{
author = "Marjanović, Žaklina and Nawaz, Ali and Stevanović, Katarina and Saljnikov, Elmira and Macek, Irena and Oehl, Fritz and Wubet, Tesfaye",
year = "2020",
abstract = "Balkan lowlands bordering with the Pannonia region are inhabited by diverse riparian forests that support production of different truffle species, predominantly the most prized white truffle of Piedmont (Tuber magnatumPico), but also other commercial species (T.macrosporumVitt.,T.aestivumVitt.). Surprisingly, little is known about the native root-associated mycobiome (RAM) of these lowland truffle-producing forests. Therefore, in this study we aim at exploring and comparing the RAMs of three different truffle-producing forests from Kolubara river plane in Serbia. Molecular methods based on next generation sequencing (NGS) were used to evaluate the diversity of root-associated fungal communities and to elucidate the influence of environmental factors on their differentiation. To our knowledge, this is the first study from such habitats with a particular focus on comparative analysis of the RAM in different truffle-producing habitats using a high-throughput sequencing approach. Our results indicated that the alpha diversity of investigated fungal communities was not significantly different between different truffle-producing forests and within a specific forest type, while the seasonal differences in the alpha diversity were only observed in the white truffle-producing forests. Taxonomic profiling at phylum level indicated the dominance of fungal OTUs belonging to phylum Ascomycota and Basidiomycota, with very minor presence of other phyla. Distinct community structures of root-associated mycobiomes were observed for white, mixed, and black truffle-producing forests. The core mycobiome analysis indicated a fair share of fungal genera present exclusively in white and black truffle-producing forest, while the core genera of mixed truffle-producing forests were shared with both white and black truffle-producing forests. The majority of detected fungal OTUs in all three forest types were symbiotrophs, with ectomycorrhizal fungi being a dominant functional guild. Apart from assumed vegetation factor, differentiation of fungal communities was driven by factors connected to the distance from the river and exposure to fluvial activities, soil age, structure, and pH. Overall, Pannonian riparian forests appear to host diverse root-associated fungal communities that are strongly shaped by variation in soil conditions.",
publisher = "MDPI, Basel",
journal = "Microorganisms",
title = "Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests",
number = "9",
volume = "8",
doi = "10.3390/microorganisms8091331"
}

Marjanović, Ž., Nawaz, A., Stevanović, K., Saljnikov, E., Macek, I., Oehl, F.,& Wubet, T.. (2020). Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests. in Microorganisms
MDPI, Basel., 8(9).
https://doi.org/10.3390/microorganisms8091331

Marjanović Ž, Nawaz A, Stevanović K, Saljnikov E, Macek I, Oehl F, Wubet T. Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests. in Microorganisms. 2020;8(9).
doi:10.3390/microorganisms8091331 .

Marjanović, Žaklina, Nawaz, Ali, Stevanović, Katarina, Saljnikov, Elmira, Macek, Irena, Oehl, Fritz, Wubet, Tesfaye, "Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests" in Microorganisms, 8, no. 9 (2020),
https://doi.org/10.3390/microorganisms8091331 . .

TY  - JOUR
AU  - Popovic-Đorđević, Jelena
AU  - Marjanović, Žaklina
AU  - Grsic, Nemanja
AU  - Adzic, Tamara
AU  - Popović, Blazenka
AU  - Bogosavljević, Jelena
AU  - Brceski, Ilija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1273
AB  - True truffles (Tuber sp.) that establish ectomycorrhizal symbiosis (ECM) with trees in the Mediterranean and temporal regions have species specific abilities to assimilate soil born elements. Suitable habitats are usually inhabited by few truffle species, while distinguishing their symbiotic potentials appeared very difficult. Two species that commonly inhabit riparian forests in Serbia are the most prized one, Tuber magnatumPico (Piedmont white truffle) and not so highly valued Tuber brumaleVitt. In order to assess potential differences between their assimilation and accumulation abilities, the differences between contents of elements that may be the subjects of the symbiotic trade between the host plant and fungi were evaluated in accumulation target (ascocarps) and their source (the soil). Essential (K, Na, Ca, Mg, Fe, P, S, and Zn) and essential trace elements (Co, Cr, Cu, Mn, and Se) in truffles and soil samples were determined by means of inductively coupled plasma with optical emission spectrometry (ICP-OES). Their concentrations (mg/kg) in ascocarps were in the range from 1.364 +/- 0.591 (Cr) to 10760.862 +/- 16.058 (K), while in soil ranged from 23.035 +/- 0.010 (Cr) to 20809.300 +/- 122.934 (Fe). Element accumulation potential (bioaccumulation factor) was calculated in the system truffle/soil. The statistical approaches were used for establishing the differences, while the possible differentiation between symbiotic potentials of two mycelia in the defined soil conditions was discussed.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Essential Elements as a Distinguishing Factor between Mycorrhizal Potentials of Two Cohabiting Truffle Species in Riparian Forest Habitat in Serbia
IS  - 4
VL  - 16
DO  - 10.1002/cbdv.201800693
ER  - 

@article{
author = "Popovic-Đorđević, Jelena and Marjanović, Žaklina and Grsic, Nemanja and Adzic, Tamara and Popović, Blazenka and Bogosavljević, Jelena and Brceski, Ilija",
year = "2019",
abstract = "True truffles (Tuber sp.) that establish ectomycorrhizal symbiosis (ECM) with trees in the Mediterranean and temporal regions have species specific abilities to assimilate soil born elements. Suitable habitats are usually inhabited by few truffle species, while distinguishing their symbiotic potentials appeared very difficult. Two species that commonly inhabit riparian forests in Serbia are the most prized one, Tuber magnatumPico (Piedmont white truffle) and not so highly valued Tuber brumaleVitt. In order to assess potential differences between their assimilation and accumulation abilities, the differences between contents of elements that may be the subjects of the symbiotic trade between the host plant and fungi were evaluated in accumulation target (ascocarps) and their source (the soil). Essential (K, Na, Ca, Mg, Fe, P, S, and Zn) and essential trace elements (Co, Cr, Cu, Mn, and Se) in truffles and soil samples were determined by means of inductively coupled plasma with optical emission spectrometry (ICP-OES). Their concentrations (mg/kg) in ascocarps were in the range from 1.364 +/- 0.591 (Cr) to 10760.862 +/- 16.058 (K), while in soil ranged from 23.035 +/- 0.010 (Cr) to 20809.300 +/- 122.934 (Fe). Element accumulation potential (bioaccumulation factor) was calculated in the system truffle/soil. The statistical approaches were used for establishing the differences, while the possible differentiation between symbiotic potentials of two mycelia in the defined soil conditions was discussed.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Essential Elements as a Distinguishing Factor between Mycorrhizal Potentials of Two Cohabiting Truffle Species in Riparian Forest Habitat in Serbia",
number = "4",
volume = "16",
doi = "10.1002/cbdv.201800693"
}

Popovic-Đorđević, J., Marjanović, Ž., Grsic, N., Adzic, T., Popović, B., Bogosavljević, J.,& Brceski, I.. (2019). Essential Elements as a Distinguishing Factor between Mycorrhizal Potentials of Two Cohabiting Truffle Species in Riparian Forest Habitat in Serbia. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 16(4).
https://doi.org/10.1002/cbdv.201800693

Popovic-Đorđević J, Marjanović Ž, Grsic N, Adzic T, Popović B, Bogosavljević J, Brceski I. Essential Elements as a Distinguishing Factor between Mycorrhizal Potentials of Two Cohabiting Truffle Species in Riparian Forest Habitat in Serbia. in Chemistry & Biodiversity. 2019;16(4).
doi:10.1002/cbdv.201800693 .

Popovic-Đorđević, Jelena, Marjanović, Žaklina, Grsic, Nemanja, Adzic, Tamara, Popović, Blazenka, Bogosavljević, Jelena, Brceski, Ilija, "Essential Elements as a Distinguishing Factor between Mycorrhizal Potentials of Two Cohabiting Truffle Species in Riparian Forest Habitat in Serbia" in Chemistry & Biodiversity, 16, no. 4 (2019),
https://doi.org/10.1002/cbdv.201800693 . .

TY  - JOUR
AU  - Mandic, Radomir
AU  - Adzemovic. Mesud
AU  - Marjanović, Žaklina
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1139
AB  - Wild edible mushrooms have received significant scientific and socio-economic attention in the last few decades, since they have become the subject of a booming trade business. Through last decades, Serbia, a small country positioned in the South East of Europe, has become a source country for extensive export of commercially important species of wild mushrooms. The data used for international analyses of national policy on mushroom protection and trade are cited usually from personal communications and therefore are not really reliable. Extensive investigations into diversity or ecology of macro fungi in Serbia have never been undertaken. The forestry sector, which is managing all forests in the country, has absolutely neglected its role in ecosystems while habitats of macro fungi have been permanently destroyed. There are only two legal acts that refer to mushroom protection directly and none aims to protect their habitats or diversity in practice. In this contribution, a comprehensive review of official data on research, conservation, socio-economic importance and legislation on wild edible mushrooms and truffles in Serbia was provided. Additionally, the application of existing legal acts on conservation of macro fungi and data on wild mushroom trade in the period between 1993-2016, during which time the trade control has been initiated was analysed. The currently valid system of conservation and trade control are discussed in the frame of protection of wild mushroom species and their habitats and measures for upgrading this system in order to meet the requirements of the sustainable use of natural resources in the socio-economic conditions of Serbia are proposed.
PB  - Pensoft Publishers, Sofia
T2  - Nature Conservation-Bulgaria
T1  - Conservation and trade of wild edible mushrooms of Serbia - history, state of the art and perspectives
EP  - 53
IS  - 25
SP  - 31
DO  - 10.3897/natureconservation.25.21919
ER  - 

@article{
author = "Mandic, Radomir and Adzemovic. Mesud and Marjanović, Žaklina",
year = "2018",
abstract = "Wild edible mushrooms have received significant scientific and socio-economic attention in the last few decades, since they have become the subject of a booming trade business. Through last decades, Serbia, a small country positioned in the South East of Europe, has become a source country for extensive export of commercially important species of wild mushrooms. The data used for international analyses of national policy on mushroom protection and trade are cited usually from personal communications and therefore are not really reliable. Extensive investigations into diversity or ecology of macro fungi in Serbia have never been undertaken. The forestry sector, which is managing all forests in the country, has absolutely neglected its role in ecosystems while habitats of macro fungi have been permanently destroyed. There are only two legal acts that refer to mushroom protection directly and none aims to protect their habitats or diversity in practice. In this contribution, a comprehensive review of official data on research, conservation, socio-economic importance and legislation on wild edible mushrooms and truffles in Serbia was provided. Additionally, the application of existing legal acts on conservation of macro fungi and data on wild mushroom trade in the period between 1993-2016, during which time the trade control has been initiated was analysed. The currently valid system of conservation and trade control are discussed in the frame of protection of wild mushroom species and their habitats and measures for upgrading this system in order to meet the requirements of the sustainable use of natural resources in the socio-economic conditions of Serbia are proposed.",
publisher = "Pensoft Publishers, Sofia",
journal = "Nature Conservation-Bulgaria",
title = "Conservation and trade of wild edible mushrooms of Serbia - history, state of the art and perspectives",
pages = "53-31",
number = "25",
doi = "10.3897/natureconservation.25.21919"
}

Mandic, R., Adzemovic. Mesud,& Marjanović, Ž.. (2018). Conservation and trade of wild edible mushrooms of Serbia - history, state of the art and perspectives. in Nature Conservation-Bulgaria
Pensoft Publishers, Sofia.(25), 31-53.
https://doi.org/10.3897/natureconservation.25.21919

Mandic R, Adzemovic. Mesud, Marjanović Ž. Conservation and trade of wild edible mushrooms of Serbia - history, state of the art and perspectives. in Nature Conservation-Bulgaria. 2018;(25):31-53.
doi:10.3897/natureconservation.25.21919 .

Mandic, Radomir, Adzemovic. Mesud, Marjanović, Žaklina, "Conservation and trade of wild edible mushrooms of Serbia - history, state of the art and perspectives" in Nature Conservation-Bulgaria, no. 25 (2018):31-53,
https://doi.org/10.3897/natureconservation.25.21919 . .

TY  - JOUR
AU  - Marjanović, Žaklina
AU  - Glisic, A.
AU  - Mutavdžić, Dragosav
AU  - Saljnikov, Elmira
AU  - Bragato, Gilberto
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/855
AB  - The production of Tuber magnatum Pico, the most prized and ecologically the most complex, constantly declines in natural habitats, while the success in plantation lacks. Contrary to the data from its habitats in Mediterranean sites, in Serbia this truffle colonizes typical developed alluvial forests, implying that dynamics of soil parameters and nutrient availability, rather than presence of specific ectomycorrhizal (EMC) plant host species or climate, might be crucial for supporting truffle life cycle. In order to reveal specific relationship generating soil microenvironments in productive and unproductive sites, soil water content (SWC) and temperature (T) were measured periodically in two depths, in a typical T. magnatum habitat in Western Serbia and compared to the atmospheric parameters (precipitation, air temperature). In three key time-points (spring soil water saturation, summer drought and autumn ascocarp production) soil was sampled through the profile and analyzed for nutrients that might be important for plant/fungal growth and ectomycorrhiza establishment. Results revealed that specific dynamics of soil water, aeration, available P and N, and possibly vegetation phenology, might be determining productivity of the microsites within the productive area. The hypothesis on soil water and nutrient availability seasonal dynamics, which might be controlling truffle establishment and life cycle completion in investigated area, was proposed.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Soil Ecology
T1  - Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion
EP  - 190
SP  - 179
VL  - 95
DO  - 10.1016/j.apsoil.2015.05.007
ER  - 

@article{
author = "Marjanović, Žaklina and Glisic, A. and Mutavdžić, Dragosav and Saljnikov, Elmira and Bragato, Gilberto",
year = "2015",
abstract = "The production of Tuber magnatum Pico, the most prized and ecologically the most complex, constantly declines in natural habitats, while the success in plantation lacks. Contrary to the data from its habitats in Mediterranean sites, in Serbia this truffle colonizes typical developed alluvial forests, implying that dynamics of soil parameters and nutrient availability, rather than presence of specific ectomycorrhizal (EMC) plant host species or climate, might be crucial for supporting truffle life cycle. In order to reveal specific relationship generating soil microenvironments in productive and unproductive sites, soil water content (SWC) and temperature (T) were measured periodically in two depths, in a typical T. magnatum habitat in Western Serbia and compared to the atmospheric parameters (precipitation, air temperature). In three key time-points (spring soil water saturation, summer drought and autumn ascocarp production) soil was sampled through the profile and analyzed for nutrients that might be important for plant/fungal growth and ectomycorrhiza establishment. Results revealed that specific dynamics of soil water, aeration, available P and N, and possibly vegetation phenology, might be determining productivity of the microsites within the productive area. The hypothesis on soil water and nutrient availability seasonal dynamics, which might be controlling truffle establishment and life cycle completion in investigated area, was proposed.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Soil Ecology",
title = "Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion",
pages = "190-179",
volume = "95",
doi = "10.1016/j.apsoil.2015.05.007"
}

Marjanović, Ž., Glisic, A., Mutavdžić, D., Saljnikov, E.,& Bragato, G.. (2015). Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion. in Applied Soil Ecology
Elsevier Science Bv, Amsterdam., 95, 179-190.
https://doi.org/10.1016/j.apsoil.2015.05.007

Marjanović Ž, Glisic A, Mutavdžić D, Saljnikov E, Bragato G. Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion. in Applied Soil Ecology. 2015;95:179-190.
doi:10.1016/j.apsoil.2015.05.007 .

Marjanović, Žaklina, Glisic, A., Mutavdžić, Dragosav, Saljnikov, Elmira, Bragato, Gilberto, "Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion" in Applied Soil Ecology, 95 (2015):179-190,
https://doi.org/10.1016/j.apsoil.2015.05.007 . .

TY  - JOUR
AU  - Beara, Ivana N.
AU  - Lesjak, Marija M.
AU  - Cetojevic-Simin, Dragana D.
AU  - Marjanović, Žaklina
AU  - Ristic, Jelena D.
AU  - Mrkonjic, Zorica O.
AU  - Mimica-Dukic, Neda M.
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/780
AB  - The aim of this study was a comprehensive investigation on phenolic profile, antioxidant, anti-inflammatory and cytotoxic activity of the still insufficiently explored black summer truffles (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles. Methanol and water extracts, obtained by maceration and Soxhlet extraction, were examined. Forty-five phenolics was studied using LC-MS/MS: presence of 14 compounds was confirmed, with the most dominant being p-hydroxybenzoic acid, baicalein and kaempferol (T. aestivum), epicatechin and catechin (T. magnatum). Moderate antioxidant activity of both species was determined through several assays. Only T. magnatum showed anti-inflammatory potential by inhibiting COX-1 and 12-LOX pathway products synthesis. Methanol extracts exerted cytotoxicity against some tumour cell lines (HeLa, MCF7, HT-29), besides the prominent activity of water extracts towards breast adenocarcinoma (MCF7). To conclude, these results support further investigations of phytochemicals and biological activity towards verification of nutraceutical use of both species.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of black (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles
EP  - 466
SP  - 460
VL  - 165
DO  - 10.1016/j.foodchem.2014.05.116
ER  - 

@article{
author = "Beara, Ivana N. and Lesjak, Marija M. and Cetojevic-Simin, Dragana D. and Marjanović, Žaklina and Ristic, Jelena D. and Mrkonjic, Zorica O. and Mimica-Dukic, Neda M.",
year = "2014",
abstract = "The aim of this study was a comprehensive investigation on phenolic profile, antioxidant, anti-inflammatory and cytotoxic activity of the still insufficiently explored black summer truffles (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles. Methanol and water extracts, obtained by maceration and Soxhlet extraction, were examined. Forty-five phenolics was studied using LC-MS/MS: presence of 14 compounds was confirmed, with the most dominant being p-hydroxybenzoic acid, baicalein and kaempferol (T. aestivum), epicatechin and catechin (T. magnatum). Moderate antioxidant activity of both species was determined through several assays. Only T. magnatum showed anti-inflammatory potential by inhibiting COX-1 and 12-LOX pathway products synthesis. Methanol extracts exerted cytotoxicity against some tumour cell lines (HeLa, MCF7, HT-29), besides the prominent activity of water extracts towards breast adenocarcinoma (MCF7). To conclude, these results support further investigations of phytochemicals and biological activity towards verification of nutraceutical use of both species.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of black (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles",
pages = "466-460",
volume = "165",
doi = "10.1016/j.foodchem.2014.05.116"
}

Beara, I. N., Lesjak, M. M., Cetojevic-Simin, D. D., Marjanović, Ž., Ristic, J. D., Mrkonjic, Z. O.,& Mimica-Dukic, N. M.. (2014). Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of black (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles. in Food Chemistry
Elsevier Sci Ltd, Oxford., 165, 460-466.
https://doi.org/10.1016/j.foodchem.2014.05.116

Beara IN, Lesjak MM, Cetojevic-Simin DD, Marjanović Ž, Ristic JD, Mrkonjic ZO, Mimica-Dukic NM. Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of black (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles. in Food Chemistry. 2014;165:460-466.
doi:10.1016/j.foodchem.2014.05.116 .

Beara, Ivana N., Lesjak, Marija M., Cetojevic-Simin, Dragana D., Marjanović, Žaklina, Ristic, Jelena D., Mrkonjic, Zorica O., Mimica-Dukic, Neda M., "Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of black (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles" in Food Chemistry, 165 (2014):460-466,
https://doi.org/10.1016/j.foodchem.2014.05.116 . .

TY  - JOUR
AU  - Pavić, Aleksandar B
AU  - Stanković, Slaviša
AU  - Saljnikov, Elmira
AU  - Krueger, Dirk
AU  - Buscot, Francois
AU  - Tarkka, Mika
AU  - Marjanović, Žaklina
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/684
AB  - To test the hypothesis that truffle-associated bacteria may improve truffle nutrition, we isolated bacteria from white truffle ascocarps and tested Actinobacteria for their ability to solubilise phosphate and iron, nutrients that have limited availability in white truffle grounds. Two isolates with sequence similarities to Curtobacterium flaccumfaciens and Rhodococcus sp. were characterized in detail. Both solubilised Ca-3(PO4)(2) in a way that was dependent on the nitrogen and carbon sources present. Neither strain broke down phytate, but both produced chelating compounds, performed ammonification, and broke down beta-glucan. Additionally, C. flaccumfaciens decomposed chitin, pectin, lipids and proteins, while Rhodococcus sp. exhibited urease activity. Three potentially fungicolous fungi were isolated from diseased white truffle ascocarps and bioassayed against the isolated Actinobacteria. The Rhodococcus isolate inhibited Verticillium leptobactrum, neither bacterium affected Clonostachys rosea, while both isolates promoted growth of Trichoderma sp. The results suggest that Actinobacteria might be involved in improving truffle nutrition, ascocarp degradation and establishing relationships with other soil fungi.
PB  - Elsevier Sci Ltd, Oxford
T2  - Fungal Ecology
T1  - Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi
EP  - 538
IS  - 6
SP  - 527
VL  - 6
DO  - 10.1016/j.funeco.2013.05.006
ER  - 

@article{
author = "Pavić, Aleksandar B and Stanković, Slaviša and Saljnikov, Elmira and Krueger, Dirk and Buscot, Francois and Tarkka, Mika and Marjanović, Žaklina",
year = "2013",
abstract = "To test the hypothesis that truffle-associated bacteria may improve truffle nutrition, we isolated bacteria from white truffle ascocarps and tested Actinobacteria for their ability to solubilise phosphate and iron, nutrients that have limited availability in white truffle grounds. Two isolates with sequence similarities to Curtobacterium flaccumfaciens and Rhodococcus sp. were characterized in detail. Both solubilised Ca-3(PO4)(2) in a way that was dependent on the nitrogen and carbon sources present. Neither strain broke down phytate, but both produced chelating compounds, performed ammonification, and broke down beta-glucan. Additionally, C. flaccumfaciens decomposed chitin, pectin, lipids and proteins, while Rhodococcus sp. exhibited urease activity. Three potentially fungicolous fungi were isolated from diseased white truffle ascocarps and bioassayed against the isolated Actinobacteria. The Rhodococcus isolate inhibited Verticillium leptobactrum, neither bacterium affected Clonostachys rosea, while both isolates promoted growth of Trichoderma sp. The results suggest that Actinobacteria might be involved in improving truffle nutrition, ascocarp degradation and establishing relationships with other soil fungi.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Fungal Ecology",
title = "Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi",
pages = "538-527",
number = "6",
volume = "6",
doi = "10.1016/j.funeco.2013.05.006"
}

Pavić, A. B., Stanković, S., Saljnikov, E., Krueger, D., Buscot, F., Tarkka, M.,& Marjanović, Ž.. (2013). Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi. in Fungal Ecology
Elsevier Sci Ltd, Oxford., 6(6), 527-538.
https://doi.org/10.1016/j.funeco.2013.05.006

Pavić AB, Stanković S, Saljnikov E, Krueger D, Buscot F, Tarkka M, Marjanović Ž. Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi. in Fungal Ecology. 2013;6(6):527-538.
doi:10.1016/j.funeco.2013.05.006 .

Pavić, Aleksandar B, Stanković, Slaviša, Saljnikov, Elmira, Krueger, Dirk, Buscot, Francois, Tarkka, Mika, Marjanović, Žaklina, "Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi" in Fungal Ecology, 6, no. 6 (2013):527-538,
https://doi.org/10.1016/j.funeco.2013.05.006 . .

TY  - JOUR
AU  - Pavić, Aleksandar B
AU  - Stanković, Slaviša
AU  - Marjanović, Žaklina
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/454
AB  - Available information on bacteria that influence the economically important white truffle (Tuber magnatum Pico) life cycle is scarce. From the ascocarp of white truffle we isolated a strain TMG 022C, capable for growth in nitrogen-depleted conditions and assimilation of mannitol and trehalose. According to 16S rDNA sequence phylogeny, the strain was closely related to Sphingobium amiense. The strain had the ability to perform ammonification, reduce nitrate and solubilize Ca-3(PO4)(2), produce chitinase, lipase, phospholipase and beta-glucanase, but not cellulase, pectinase, protease and siderophores. The results suggest that Sphingobium sp. TMG 022C could have an influence on the Tuber magnatum life cycle through improved mycelium nutrition and ascocarp decomposition.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Biochemical characterization of a sphingomonad isolate from the ascocarp of white truffle (tuber magnatum pico)
EP  - 704
IS  - 3
SP  - 697
VL  - 63
DO  - 10.2298/ABS1103697P
ER  - 

@article{
author = "Pavić, Aleksandar B and Stanković, Slaviša and Marjanović, Žaklina",
year = "2011",
abstract = "Available information on bacteria that influence the economically important white truffle (Tuber magnatum Pico) life cycle is scarce. From the ascocarp of white truffle we isolated a strain TMG 022C, capable for growth in nitrogen-depleted conditions and assimilation of mannitol and trehalose. According to 16S rDNA sequence phylogeny, the strain was closely related to Sphingobium amiense. The strain had the ability to perform ammonification, reduce nitrate and solubilize Ca-3(PO4)(2), produce chitinase, lipase, phospholipase and beta-glucanase, but not cellulase, pectinase, protease and siderophores. The results suggest that Sphingobium sp. TMG 022C could have an influence on the Tuber magnatum life cycle through improved mycelium nutrition and ascocarp decomposition.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Biochemical characterization of a sphingomonad isolate from the ascocarp of white truffle (tuber magnatum pico)",
pages = "704-697",
number = "3",
volume = "63",
doi = "10.2298/ABS1103697P"
}

Pavić, A. B., Stanković, S.,& Marjanović, Ž.. (2011). Biochemical characterization of a sphingomonad isolate from the ascocarp of white truffle (tuber magnatum pico). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(3), 697-704.
https://doi.org/10.2298/ABS1103697P

Pavić AB, Stanković S, Marjanović Ž. Biochemical characterization of a sphingomonad isolate from the ascocarp of white truffle (tuber magnatum pico). in Archives of Biological Sciences. 2011;63(3):697-704.
doi:10.2298/ABS1103697P .

Pavić, Aleksandar B, Stanković, Slaviša, Marjanović, Žaklina, "Biochemical characterization of a sphingomonad isolate from the ascocarp of white truffle (tuber magnatum pico)" in Archives of Biological Sciences, 63, no. 3 (2011):697-704,
https://doi.org/10.2298/ABS1103697P . .

TY  - JOUR
AU  - Cakmak, Dragan
AU  - Saljnikov, Elmira
AU  - Mrvić, Vesna
AU  - Jakovljević, Miodrag D
AU  - Marjanović, Žaklina
AU  - Sikiric, Biljana
AU  - Maksimović, Srboljub
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/387
AB  - Long-term application of P fertilizers may eventually result: in excess and/or toxic accumulations of trace elements and microelements in soil. The effect of monoammonium-phosphate (MAP) on basic soil properties (pH, CEC, texture), the total content of C N, and F, hot acid-extractable Cu, Zn, Ni, Cr, Pb, Cd, Co, As, Hg, and F, and the content of extractable macro- and trace elements (P, K, Ca, Mg, Al, Fe, Zn, Cu, Ni, Pb, Cd, F) were Studied on a Stagnosol soil. Phosphate fertilizer had been applied (26, 39, and 52 kg P ha(-1)) over a 40-yr period. Phosphorus fertilization significantly decreased pH and increased clay content of the soil. Increases were detected in available P, exchangeable Al, Ca, cation exchange capacity (CEC), and clay content. The content of hot acid-ex tractable Pb increased, whereas the content of diethylenetriaminepentaacetic acid-extractable Pb decreased in accordance with applied rates of MAP. The status of some hot acid-extractable trace elements (Cu, Zn, Ni, and Co) did not change after 40 yr of MAP application, whereas Hg and Cd increased. However, despite the statistically significant increases in the amounts of some potentially toxic elements, they did riot accumulate to concentrations considered toxic as overall concentrations are far below the maximum allowed concentrations for natural unpolluted soils.
PB  - Wiley, Hoboken
T2  - Journal of Environmental Quality
T1  - Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization
EP  - 547
IS  - 2
SP  - 541
VL  - 39
DO  - 10.2134/jeq2009.0216
ER  - 

@article{
author = "Cakmak, Dragan and Saljnikov, Elmira and Mrvić, Vesna and Jakovljević, Miodrag D and Marjanović, Žaklina and Sikiric, Biljana and Maksimović, Srboljub",
year = "2010",
abstract = "Long-term application of P fertilizers may eventually result: in excess and/or toxic accumulations of trace elements and microelements in soil. The effect of monoammonium-phosphate (MAP) on basic soil properties (pH, CEC, texture), the total content of C N, and F, hot acid-extractable Cu, Zn, Ni, Cr, Pb, Cd, Co, As, Hg, and F, and the content of extractable macro- and trace elements (P, K, Ca, Mg, Al, Fe, Zn, Cu, Ni, Pb, Cd, F) were Studied on a Stagnosol soil. Phosphate fertilizer had been applied (26, 39, and 52 kg P ha(-1)) over a 40-yr period. Phosphorus fertilization significantly decreased pH and increased clay content of the soil. Increases were detected in available P, exchangeable Al, Ca, cation exchange capacity (CEC), and clay content. The content of hot acid-ex tractable Pb increased, whereas the content of diethylenetriaminepentaacetic acid-extractable Pb decreased in accordance with applied rates of MAP. The status of some hot acid-extractable trace elements (Cu, Zn, Ni, and Co) did not change after 40 yr of MAP application, whereas Hg and Cd increased. However, despite the statistically significant increases in the amounts of some potentially toxic elements, they did riot accumulate to concentrations considered toxic as overall concentrations are far below the maximum allowed concentrations for natural unpolluted soils.",
publisher = "Wiley, Hoboken",
journal = "Journal of Environmental Quality",
title = "Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization",
pages = "547-541",
number = "2",
volume = "39",
doi = "10.2134/jeq2009.0216"
}

Cakmak, D., Saljnikov, E., Mrvić, V., Jakovljević, M. D., Marjanović, Ž., Sikiric, B.,& Maksimović, S.. (2010). Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization. in Journal of Environmental Quality
Wiley, Hoboken., 39(2), 541-547.
https://doi.org/10.2134/jeq2009.0216

Cakmak D, Saljnikov E, Mrvić V, Jakovljević MD, Marjanović Ž, Sikiric B, Maksimović S. Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization. in Journal of Environmental Quality. 2010;39(2):541-547.
doi:10.2134/jeq2009.0216 .

Cakmak, Dragan, Saljnikov, Elmira, Mrvić, Vesna, Jakovljević, Miodrag D, Marjanović, Žaklina, Sikiric, Biljana, Maksimović, Srboljub, "Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization" in Journal of Environmental Quality, 39, no. 2 (2010):541-547,
https://doi.org/10.2134/jeq2009.0216 . .

TY  - JOUR
AU  - Marjanović, Žaklina
AU  - Grebenc, T.
AU  - Marković, M.
AU  - Glišić, A.
AU  - Milenković, Milica R.
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/391
AB  - Marjanovic, Z(1), Grebenc, T(2), Markovic, M(3), Glisic, A(4), and Milenkovic, M(5) (2010) Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula Sydowia 62 (1) 67-87 Little is known on the diversity and distribution of truffles at the Balkan Peninsula A first detailed study of hypogeous fungi in Serbia has been started at 1992 To extend the knowledge on European truffles and their natural habitats, data on their diversity in Serbia, and to some extent in Montenegro and FYRO Macedonia are summarised within this paper Twelve species of the genus Tuber, including five varieties of T rufum Pico, are recorded and their habitats briefly described Four species are reported for the Balkan Peninsula for the first time In addition, parts of ribosomal DNA (ITS regions) of 46 specimens are sequenced and analysed to confirm the morphological determinations ITS sequences of specimens morphologically identified as Tuber fulgens Quel are reported for the first time A phylogeny based on ITS sequences of the Balkan truffles and additional 29 records from the GenBank is calculated The results are discussed towards expanding the information on distribution as well as ecological and molecular diversification of Tuber spp in Europe
T2  - Sydowia
T1  - Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula
EP  - 87
IS  - 1
SP  - 67
VL  - 62
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_391
ER  - 

@article{
author = "Marjanović, Žaklina and Grebenc, T. and Marković, M. and Glišić, A. and Milenković, Milica R.",
year = "2010",
abstract = "Marjanovic, Z(1), Grebenc, T(2), Markovic, M(3), Glisic, A(4), and Milenkovic, M(5) (2010) Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula Sydowia 62 (1) 67-87 Little is known on the diversity and distribution of truffles at the Balkan Peninsula A first detailed study of hypogeous fungi in Serbia has been started at 1992 To extend the knowledge on European truffles and their natural habitats, data on their diversity in Serbia, and to some extent in Montenegro and FYRO Macedonia are summarised within this paper Twelve species of the genus Tuber, including five varieties of T rufum Pico, are recorded and their habitats briefly described Four species are reported for the Balkan Peninsula for the first time In addition, parts of ribosomal DNA (ITS regions) of 46 specimens are sequenced and analysed to confirm the morphological determinations ITS sequences of specimens morphologically identified as Tuber fulgens Quel are reported for the first time A phylogeny based on ITS sequences of the Balkan truffles and additional 29 records from the GenBank is calculated The results are discussed towards expanding the information on distribution as well as ecological and molecular diversification of Tuber spp in Europe",
journal = "Sydowia",
title = "Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula",
pages = "87-67",
number = "1",
volume = "62",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_391"
}

Marjanović, Ž., Grebenc, T., Marković, M., Glišić, A.,& Milenković, M. R.. (2010). Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula. in Sydowia, 62(1), 67-87.
https://hdl.handle.net/21.15107/rcub_rimsi_391

Marjanović Ž, Grebenc T, Marković M, Glišić A, Milenković MR. Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula. in Sydowia. 2010;62(1):67-87.
https://hdl.handle.net/21.15107/rcub_rimsi_391 .

Marjanović, Žaklina, Grebenc, T., Marković, M., Glišić, A., Milenković, Milica R., "Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula" in Sydowia, 62, no. 1 (2010):67-87,
https://hdl.handle.net/21.15107/rcub_rimsi_391 .

TY  - JOUR
AU  - Harhaji-Trajković, Ljubica M.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, Ivana D.
AU  - Momcilović, Miljana
AU  - Tufegdžić, Srđan J.
AU  - Maksimović, Vuk
AU  - Marjanović, Žaklina
AU  - Stosic-Grujicic, Stanislava
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/347
AB  - Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer-An International Journal
T1  - Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo
EP  - 707
IS  - 5
SP  - 696
VL  - 61
DO  - 10.1080/01635580902898743
ER  - 

@article{
author = "Harhaji-Trajković, Ljubica M. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, Ivana D. and Momcilović, Miljana and Tufegdžić, Srđan J. and Maksimović, Vuk and Marjanović, Žaklina and Stosic-Grujicic, Stanislava",
year = "2009",
abstract = "Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer-An International Journal",
title = "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo",
pages = "707-696",
number = "5",
volume = "61",
doi = "10.1080/01635580902898743"
}

Harhaji-Trajković, L. M., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I. D., Momcilović, M., Tufegdžić, S. J., Maksimović, V., Marjanović, Ž.,& Stosic-Grujicic, S.. (2009). Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon., 61(5), 696-707.
https://doi.org/10.1080/01635580902898743

Harhaji-Trajković LM, Mijatović S, Maksimović-Ivanić D, Stojanović ID, Momcilović M, Tufegdžić SJ, Maksimović V, Marjanović Ž, Stosic-Grujicic S. Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal. 2009;61(5):696-707.
doi:10.1080/01635580902898743 .

Harhaji-Trajković, Ljubica M., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, Ivana D., Momcilović, Miljana, Tufegdžić, Srđan J., Maksimović, Vuk, Marjanović, Žaklina, Stosic-Grujicic, Stanislava, "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo" in Nutrition and Cancer-An International Journal, 61, no. 5 (2009):696-707,
https://doi.org/10.1080/01635580902898743 . .

TY  - JOUR
AU  - Harhaji, Lj.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, I.
AU  - Momcilović, Miljana
AU  - Maksimović, Vuk
AU  - Tufegdžić, Srđan J.
AU  - Marjanović, Žaklina
AU  - Mostarica-Stojković, M.
AU  - Vučinić, Željko
AU  - Stosic-Grujicic, Stanislava
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/262
AB  - Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study
EP  - 1833
IS  - 5
SP  - 1825
VL  - 46
DO  - 10.1016/j.fct.2008.01.027
ER  - 

@article{
author = "Harhaji, Lj. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, I. and Momcilović, Miljana and Maksimović, Vuk and Tufegdžić, Srđan J. and Marjanović, Žaklina and Mostarica-Stojković, M. and Vučinić, Željko and Stosic-Grujicic, Stanislava",
year = "2008",
abstract = "Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study",
pages = "1833-1825",
number = "5",
volume = "46",
doi = "10.1016/j.fct.2008.01.027"
}

Harhaji, Lj., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I., Momcilović, M., Maksimović, V., Tufegdžić, S. J., Marjanović, Ž., Mostarica-Stojković, M., Vučinić, Ž.,& Stosic-Grujicic, S.. (2008). Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 46(5), 1825-1833.
https://doi.org/10.1016/j.fct.2008.01.027

Harhaji L, Mijatović S, Maksimović-Ivanić D, Stojanović I, Momcilović M, Maksimović V, Tufegdžić SJ, Marjanović Ž, Mostarica-Stojković M, Vučinić Ž, Stosic-Grujicic S. Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology. 2008;46(5):1825-1833.
doi:10.1016/j.fct.2008.01.027 .

Harhaji, Lj., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, I., Momcilović, Miljana, Maksimović, Vuk, Tufegdžić, Srđan J., Marjanović, Žaklina, Mostarica-Stojković, M., Vučinić, Željko, Stosic-Grujicic, Stanislava, "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study" in Food and Chemical Toxicology, 46, no. 5 (2008):1825-1833,
https://doi.org/10.1016/j.fct.2008.01.027 . .

TY  - CHAP
AU  - Marjanović, Žaklina
AU  - Nehls, U.
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/274
AB  - In temperate and boreal regions, seasons are characterized by two major factors - temperature and water availability. As water availability is affecting essential processes like nutrition and photosynthesis, it is of central importance for plant physiology. Trees and shrubs of temperate and boreal forest ecosystems are characterized by a tight association of their fine roots with certain soil fungi, forming a new symbiotic organ - the ectomycorrhiza. Here, fine roots are often covered by fungal hyphae (the so-called sheath) isolating them from the surrounding soil. Furthermore, fungal hyphae grow within the apoplast of rhizodermis and root cortex, forming a dense hyphal network (Hartig net), which is thought to function as an interface between fungus and plant for the reciprocal exchange of nutrients and metabolites. Mycorrhizas are connected with other parts of the fungal colony (e.g., soil exploring mycelium) by specialized transport hyphae (see below) and, in contrast to a number of well investigated filamentous model ascomycetes (e.g., Neurospora, Aspergillus), EM fungal colonies perform intense nutrient and metabolite exchange (for reviews, see Smith and Read 1997; Anderson and Cairney 2007).
PB  - Springer-Verlag Berlin Heidelberg
T2  - Mycorrhiza: State of the Art, Genetics and Molecular Biology, Eco-Function, Biotechnology, Eco-Physi
T1  - Ectomycorrhiza and water transport
EP  - 159
SP  - 149
DO  - 10.1007/978-3-540-78826-3_8
ER  - 

@inbook{
author = "Marjanović, Žaklina and Nehls, U.",
year = "2008",
abstract = "In temperate and boreal regions, seasons are characterized by two major factors - temperature and water availability. As water availability is affecting essential processes like nutrition and photosynthesis, it is of central importance for plant physiology. Trees and shrubs of temperate and boreal forest ecosystems are characterized by a tight association of their fine roots with certain soil fungi, forming a new symbiotic organ - the ectomycorrhiza. Here, fine roots are often covered by fungal hyphae (the so-called sheath) isolating them from the surrounding soil. Furthermore, fungal hyphae grow within the apoplast of rhizodermis and root cortex, forming a dense hyphal network (Hartig net), which is thought to function as an interface between fungus and plant for the reciprocal exchange of nutrients and metabolites. Mycorrhizas are connected with other parts of the fungal colony (e.g., soil exploring mycelium) by specialized transport hyphae (see below) and, in contrast to a number of well investigated filamentous model ascomycetes (e.g., Neurospora, Aspergillus), EM fungal colonies perform intense nutrient and metabolite exchange (for reviews, see Smith and Read 1997; Anderson and Cairney 2007).",
publisher = "Springer-Verlag Berlin Heidelberg",
journal = "Mycorrhiza: State of the Art, Genetics and Molecular Biology, Eco-Function, Biotechnology, Eco-Physi",
booktitle = "Ectomycorrhiza and water transport",
pages = "159-149",
doi = "10.1007/978-3-540-78826-3_8"
}

Marjanović, Ž.,& Nehls, U.. (2008). Ectomycorrhiza and water transport. in Mycorrhiza: State of the Art, Genetics and Molecular Biology, Eco-Function, Biotechnology, Eco-Physi
Springer-Verlag Berlin Heidelberg., 149-159.
https://doi.org/10.1007/978-3-540-78826-3_8

Marjanović Ž, Nehls U. Ectomycorrhiza and water transport. in Mycorrhiza: State of the Art, Genetics and Molecular Biology, Eco-Function, Biotechnology, Eco-Physi. 2008;:149-159.
doi:10.1007/978-3-540-78826-3_8 .

Marjanović, Žaklina, Nehls, U., "Ectomycorrhiza and water transport" in Mycorrhiza: State of the Art, Genetics and Molecular Biology, Eco-Function, Biotechnology, Eco-Physi (2008):149-159,
https://doi.org/10.1007/978-3-540-78826-3_8 . .

TY  - JOUR
AU  - Marjanović, Žaklina
AU  - Uwe, N
AU  - Hampp, R
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/131
AB  - Mycorrhized and nonmycorrhized plants of hybrid poplar (Populus tremula L. x Populus tremuloides Mich) were cultivated in petri dishes until the amount of water became limiting (detected by a decrease of photosynthetic yield). RNA was isolated from both main and fine roots and used for analysis of water channel (aquaporin) gene expression by semiquantitative RT-PCR. For three of five investigated aquaporin genes, expression did not differ between mycorrhized and nonmycorrhized plants. However, expression of the two other aquaporin genes was more pronounced in fine roots of mycorrhized plants, indicating that the root-fungus symbiosis may improve the water transport capacity under conditions of reduced water availability.
PB  - New York Acad Sciences, New York
T2  - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Mycorrhiza formation enhances adaptive response of hybrid poplar to drought
EP  - 499
SP  - 496
VL  - 1048
DO  - 10.1196/annals.1342.080
ER  - 

@article{
author = "Marjanović, Žaklina and Uwe, N and Hampp, R",
year = "2005",
abstract = "Mycorrhized and nonmycorrhized plants of hybrid poplar (Populus tremula L. x Populus tremuloides Mich) were cultivated in petri dishes until the amount of water became limiting (detected by a decrease of photosynthetic yield). RNA was isolated from both main and fine roots and used for analysis of water channel (aquaporin) gene expression by semiquantitative RT-PCR. For three of five investigated aquaporin genes, expression did not differ between mycorrhized and nonmycorrhized plants. However, expression of the two other aquaporin genes was more pronounced in fine roots of mycorrhized plants, indicating that the root-fungus symbiosis may improve the water transport capacity under conditions of reduced water availability.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Mycorrhiza formation enhances adaptive response of hybrid poplar to drought",
pages = "499-496",
volume = "1048",
doi = "10.1196/annals.1342.080"
}

Marjanović, Ž., Uwe, N.,& Hampp, R.. (2005). Mycorrhiza formation enhances adaptive response of hybrid poplar to drought. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 496-499.
https://doi.org/10.1196/annals.1342.080

Marjanović Ž, Uwe N, Hampp R. Mycorrhiza formation enhances adaptive response of hybrid poplar to drought. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:496-499.
doi:10.1196/annals.1342.080 .

Marjanović, Žaklina, Uwe, N, Hampp, R, "Mycorrhiza formation enhances adaptive response of hybrid poplar to drought" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):496-499,
https://doi.org/10.1196/annals.1342.080 . .

TY  - JOUR
AU  - Marjanović, Žaklina
AU  - Uehlein, N
AU  - Kaldenhoff, R
AU  - Zwiazek, JJ
AU  - Weiss, M
AU  - Hampp, R
AU  - Nehls, U
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/121
AB  - The formation of ectomycorrhizas, a tight association between fine roots of trees and certain soil fungi, improves plant nutrition in a nutrient-limited environment and may increase plant survival under water stress conditions. To investigate the impact of mycorrhiza formation on plant water uptake, seven genes coding for putative water channel proteins (aquaporins) were isolated from a poplar ectomycorrhizal cDNA library. Four out of the seven genes were preferentially expressed in roots. Mycorrhiza formation resulted in an increased transcript level for three of these genes, two of which are the most prominently expressed aquaporins in roots. When expressed in Xenopus laevis oocytes, the corresponding proteins of both genes were able to transport water. Together, these data indicate, that the water transport capacity of the plasma membrane of root cells is strongly increased in mycorrhized plants. Measurements of the hydraulic conductance of intact root systems revealed an increased water transport capacity of mycorrhized poplar roots. These data, however, also indicate that changes in the properties of the plasma membrane as well as those of the apoplast are responsible for the increased root hydraulic conductance in ectomycorrhizal symbiosis.
PB  - Springer, New York
T2  - Planta
T1  - Aquaporins in poplar: What a difference a symbiont makes!
EP  - 268
IS  - 2
SP  - 258
VL  - 222
DO  - 10.1007/s00425-005-1539-z
ER  - 

@article{
author = "Marjanović, Žaklina and Uehlein, N and Kaldenhoff, R and Zwiazek, JJ and Weiss, M and Hampp, R and Nehls, U",
year = "2005",
abstract = "The formation of ectomycorrhizas, a tight association between fine roots of trees and certain soil fungi, improves plant nutrition in a nutrient-limited environment and may increase plant survival under water stress conditions. To investigate the impact of mycorrhiza formation on plant water uptake, seven genes coding for putative water channel proteins (aquaporins) were isolated from a poplar ectomycorrhizal cDNA library. Four out of the seven genes were preferentially expressed in roots. Mycorrhiza formation resulted in an increased transcript level for three of these genes, two of which are the most prominently expressed aquaporins in roots. When expressed in Xenopus laevis oocytes, the corresponding proteins of both genes were able to transport water. Together, these data indicate, that the water transport capacity of the plasma membrane of root cells is strongly increased in mycorrhized plants. Measurements of the hydraulic conductance of intact root systems revealed an increased water transport capacity of mycorrhized poplar roots. These data, however, also indicate that changes in the properties of the plasma membrane as well as those of the apoplast are responsible for the increased root hydraulic conductance in ectomycorrhizal symbiosis.",
publisher = "Springer, New York",
journal = "Planta",
title = "Aquaporins in poplar: What a difference a symbiont makes!",
pages = "268-258",
number = "2",
volume = "222",
doi = "10.1007/s00425-005-1539-z"
}

Marjanović, Ž., Uehlein, N., Kaldenhoff, R., Zwiazek, J., Weiss, M., Hampp, R.,& Nehls, U.. (2005). Aquaporins in poplar: What a difference a symbiont makes!. in Planta
Springer, New York., 222(2), 258-268.
https://doi.org/10.1007/s00425-005-1539-z

Marjanović Ž, Uehlein N, Kaldenhoff R, Zwiazek J, Weiss M, Hampp R, Nehls U. Aquaporins in poplar: What a difference a symbiont makes!. in Planta. 2005;222(2):258-268.
doi:10.1007/s00425-005-1539-z .

Marjanović, Žaklina, Uehlein, N, Kaldenhoff, R, Zwiazek, JJ, Weiss, M, Hampp, R, Nehls, U, "Aquaporins in poplar: What a difference a symbiont makes!" in Planta, 222, no. 2 (2005):258-268,
https://doi.org/10.1007/s00425-005-1539-z . .