TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Žižić, Milan
AU  - Dučić, Tanja
AU  - AQUILANTI, Giuliana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Danilović Luković, Jelena
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2378
AB  - Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.
PB  - Oxford University Press
T2  - Journal of Experimental Botany
T1  - A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana
EP  - 1122
IS  - 3
SP  - 1107
VL  - 74
DO  - 10.1093/jxb/erac472
ER  - 

@article{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Žižić, Milan and Dučić, Tanja and AQUILANTI, Giuliana and Stanić, Marina and Zechmann, Bernd and Danilović Luković, Jelena and Stanković, Dalibor M. and Opačić, Miloš and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2023",
abstract = "Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.",
publisher = "Oxford University Press",
journal = "Journal of Experimental Botany",
title = "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana",
pages = "1122-1107",
number = "3",
volume = "74",
doi = "10.1093/jxb/erac472"
}

Vojvodić, S., Dimitrijević, M., Žižić, M., Dučić, T., AQUILANTI, G., Stanić, M., Zechmann, B., Danilović Luković, J., Stanković, D. M., Opačić, M., Morina, A., Pittman, J. K.,& Spasojević, I.. (2023). A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany
Oxford University Press., 74(3), 1107-1122.
https://doi.org/10.1093/jxb/erac472

Vojvodić S, Dimitrijević M, Žižić M, Dučić T, AQUILANTI G, Stanić M, Zechmann B, Danilović Luković J, Stanković DM, Opačić M, Morina A, Pittman JK, Spasojević I. A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany. 2023;74(3):1107-1122.
doi:10.1093/jxb/erac472 .

Vojvodić, Snežana, Dimitrijević, Milena, Žižić, Milan, Dučić, Tanja, AQUILANTI, Giuliana, Stanić, Marina, Zechmann, Bernd, Danilović Luković, Jelena, Stanković, Dalibor M., Opačić, Miloš, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana" in Journal of Experimental Botany, 74, no. 3 (2023):1107-1122,
https://doi.org/10.1093/jxb/erac472 . .

TY  - JOUR
AU  - Dučić, Tanja
AU  - Alves, Carla
AU  - Vučinić, Željko
AU  - Lázaro-Martínez, Juan
AU  - Petković, Marijana
AU  - Soto, Juan
AU  - Mutavdžić, Dragosav
AU  - Martinez de Yuso Garcia, Maria del Valle
AU  - Radotić, Ksenija
AU  - Algarra, Manuel
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1558
AB  - S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives.
(S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells
resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and
N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption,
ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition,
synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical
changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively.
Computational chemistry was applied to establish the model for the most stable bond between
S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with
cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells
led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The
results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat
the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.
PB  - Elsevier
T2  - Journal of Colloid and Interface Science
T1  - S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach
EP  - 237
IS  - 623
SP  - 226
DO  - 10.1016/j.jcis.2022.05.005
ER  - 

@article{
author = "Dučić, Tanja and Alves, Carla and Vučinić, Željko and Lázaro-Martínez, Juan and Petković, Marijana and Soto, Juan and Mutavdžić, Dragosav and Martinez de Yuso Garcia, Maria del Valle and Radotić, Ksenija and Algarra, Manuel",
year = "2022",
abstract = "S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives.
(S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells
resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and
N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption,
ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition,
synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical
changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively.
Computational chemistry was applied to establish the model for the most stable bond between
S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with
cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells
led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The
results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat
the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.",
publisher = "Elsevier",
journal = "Journal of Colloid and Interface Science",
title = "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach",
pages = "237-226",
number = "623",
doi = "10.1016/j.jcis.2022.05.005"
}

Dučić, T., Alves, C., Vučinić, Ž., Lázaro-Martínez, J., Petković, M., Soto, J., Mutavdžić, D., Martinez de Yuso Garcia, M. d. V., Radotić, K.,& Algarra, M.. (2022). S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science
Elsevier.(623), 226-237.
https://doi.org/10.1016/j.jcis.2022.05.005

Dučić T, Alves C, Vučinić Ž, Lázaro-Martínez J, Petković M, Soto J, Mutavdžić D, Martinez de Yuso Garcia MDV, Radotić K, Algarra M. S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science. 2022;(623):226-237.
doi:10.1016/j.jcis.2022.05.005 .

Dučić, Tanja, Alves, Carla, Vučinić, Željko, Lázaro-Martínez, Juan, Petković, Marijana, Soto, Juan, Mutavdžić, Dragosav, Martinez de Yuso Garcia, Maria del Valle, Radotić, Ksenija, Algarra, Manuel, "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach" in Journal of Colloid and Interface Science, no. 623 (2022):226-237,
https://doi.org/10.1016/j.jcis.2022.05.005 . .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Dučić, Tanja
AU  - Stanković, Dalibor
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2391
AB  - Microalgae can be implemented in the remediation of mining and other metal-rich wastewaters as pioneer species. On the other hand, metals affect aquatic ecosystems through the negative impact on microalgae which are the primary producers of O2 and biomass. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. We analyzed the redox changes in Chlorella sorokiniana culture that are induced by high levels of Mn(II). Mn is the key metal pollutant, with five main oxidation forms that can bind to a variety of different ligands. Mn (1 mM) induced a significant increase in the intracellular production of reactive oxygen species. The boost appears to show two phases – the first is very fast (observed after 15 min), whereas the second starts after 1 h reaching a plateau at 24 h. The concentration of reduced thiols, which represent important targets of oxidation, appears to parallel this trend. Total glutathione concentration shows a drop at 1 h and recovery at 24 h. This implicates that either a glutathionylation of proteins or a synthesis of phytochelatins - sulfur-rich short-chain peptides that sequester metals, takes place early in the response to Mn. Further, FTIR analysis showed that Mn induced a decrease of C=C levels and CH2/CH3 ratio implicating increased lipid peroxidation. Finally, Mn ions that were accumulated in the cells were extracted with nitric oxide and analyzed by cyclic voltammetry. Two redox forms were detected - Mn(II) and Mn(IV). The latter appears to prevail at higher manganese concentrations and longer periods of incubation. These results demonstrate that redox response of C. sorokiniana to high Mn levels involves at least two phases. Initially, Mn(II) enters the cells and induces pro-oxidative changes that are mitigated by glutathione-based antioxidative defense. Later on, redox homeostasis is reestablished with concomitant inactivation of Mn in the more stable redox form.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
T1  - Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)
EP  - 174
SP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2391
ER  - 

@conference{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Dučić, Tanja and Stanković, Dalibor and Opačić, Miloš and Stanić, Marina and Žižić, Milan and Spasojević, Ivan",
year = "2021",
abstract = "Microalgae can be implemented in the remediation of mining and other metal-rich wastewaters as pioneer species. On the other hand, metals affect aquatic ecosystems through the negative impact on microalgae which are the primary producers of O2 and biomass. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. We analyzed the redox changes in Chlorella sorokiniana culture that are induced by high levels of Mn(II). Mn is the key metal pollutant, with five main oxidation forms that can bind to a variety of different ligands. Mn (1 mM) induced a significant increase in the intracellular production of reactive oxygen species. The boost appears to show two phases – the first is very fast (observed after 15 min), whereas the second starts after 1 h reaching a plateau at 24 h. The concentration of reduced thiols, which represent important targets of oxidation, appears to parallel this trend. Total glutathione concentration shows a drop at 1 h and recovery at 24 h. This implicates that either a glutathionylation of proteins or a synthesis of phytochelatins - sulfur-rich short-chain peptides that sequester metals, takes place early in the response to Mn. Further, FTIR analysis showed that Mn induced a decrease of C=C levels and CH2/CH3 ratio implicating increased lipid peroxidation. Finally, Mn ions that were accumulated in the cells were extracted with nitric oxide and analyzed by cyclic voltammetry. Two redox forms were detected - Mn(II) and Mn(IV). The latter appears to prevail at higher manganese concentrations and longer periods of incubation. These results demonstrate that redox response of C. sorokiniana to high Mn levels involves at least two phases. Initially, Mn(II) enters the cells and induces pro-oxidative changes that are mitigated by glutathione-based antioxidative defense. Later on, redox homeostasis is reestablished with concomitant inactivation of Mn in the more stable redox form.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia",
title = "Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)",
pages = "174-174",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2391"
}

Vojvodić, S., Dimitrijević, M., Dučić, T., Stanković, D., Opačić, M., Stanić, M., Žižić, M.,& Spasojević, I.. (2021). Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II). in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 174-174.
https://hdl.handle.net/21.15107/rcub_rimsi_2391

Vojvodić S, Dimitrijević M, Dučić T, Stanković D, Opačić M, Stanić M, Žižić M, Spasojević I. Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II). in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia. 2021;:174-174.
https://hdl.handle.net/21.15107/rcub_rimsi_2391 .

Vojvodić, Snežana, Dimitrijević, Milena, Dučić, Tanja, Stanković, Dalibor, Opačić, Miloš, Stanić, Marina, Žižić, Milan, Spasojević, Ivan, "Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)" in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia (2021):174-174,
https://hdl.handle.net/21.15107/rcub_rimsi_2391 .