@conference{
author = "Mitrović, Aleksandra Lj. and Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Steinbach, Gabor and Janošević, Dušica and Vuković, Marina and Garab, Gyozo and Radotić, Ksenija",
year = "2018",
abstract = "Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues:
fibers, vessel elements and tracheids. The contribution of parenchyma cells to stem twining
is mostly unknown. Plants control the orientation and alignment of cellulose fibrils during the
deposition in the cell walls with high precision, creating required anisotropy of the cell wall. Our
aim was to determine possible changes in cellulose fibrils orientation and structural order in stem
parenchyma cell walls related to stem twinning in liana plants.
We applied different microscopy techniques: light microscopy, scanning electron microscopy
and differential polarization laser scanning microscopy (DP-LSM) for fluorescence detected linear
dichroism imaging (FDLD), on stem cross sections of straight and twisted internodes of monocotyledonous
liana Dioscorea balcanica. Histochemical analysis showed no difference in parenchyma
cell wall structure between straight and twisted internodes. Also, no difference in ‘‘cellulose fiber
order’’ in parenchyma cell walls related to stem twining was found by FDLD microscopy. However,
SEM micrographs suggested the difference in cellulose microfibril orientation in secondary cell
walls of parenchyma cells related to stem twining.
Our results indicate that adaptations to stem twining in liana plants involve modifications in
cellulose microfibril orientation in parenchyma cell walls. Although the orientation of cellulose
microfibrils dictates, among other properties, cell shape, living stem parenchyma cells in D. balcanica
retain their shape regardless of stem twining, which is possibly enabled by retaining ‘‘cellulose
fibril order’’.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research "Siniša Stankovic", University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Modifications in parenchyma cell wall structure related to stem twining in monocotyledonous liana Dioscorea balcanica Košanin",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2987"
}