TY  - JOUR
AU  - Radotić, Ksenija
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Natić, Maja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2477
AB  - Food is a complex matrix of proteins, fats, minerals, vitamins, and other components. Various analytical methods are currently used for food testing. However, most of the used methods require sample preprocessing and expensive chemicals. New analytical methods are needed for quick and economic measurement of food quality and safety. Fluorescence spectroscopy is a simple and quick method to measure food quality, without sample preprocessing. This technique has been developed for food samples due to the application of a front-face measuring setup. Fluorescent compounds–fluorophores in the food samples are highly sensitive to their environment. Information about molecular structure and changes in food samples is obtained by the measurement of excitation–emission matrices of the endogenous fluorophores and by applying multivariate chemometric tools. Synchronous fluorescence spectroscopy is an advantageous screening mode used in food analysis. The fluorescent markers in food are amino acids tryptophan and tyrosine; the structural proteins collagen and elastin; the enzymes and co-enzymes NADH and FAD; vitamins; lipids; porphyrins; and mycotoxins in certain food types. The review provides information on the principles of the fluorescence measurements of food samples and the advantages of this method over the others. An analysis of the fluorescence spectroscopy applications in screening the various food types is provided.
PB  - MDPI
T2  - Foods
T1  - Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach
EP  - 17
IS  - 16
SP  - 1
VL  - 12
DO  - 10.3390/foods12163023
ER  - 

@article{
author = "Radotić, Ksenija and Stanković, Mira and Bartolić, Dragana and Natić, Maja",
year = "2023",
abstract = "Food is a complex matrix of proteins, fats, minerals, vitamins, and other components. Various analytical methods are currently used for food testing. However, most of the used methods require sample preprocessing and expensive chemicals. New analytical methods are needed for quick and economic measurement of food quality and safety. Fluorescence spectroscopy is a simple and quick method to measure food quality, without sample preprocessing. This technique has been developed for food samples due to the application of a front-face measuring setup. Fluorescent compounds–fluorophores in the food samples are highly sensitive to their environment. Information about molecular structure and changes in food samples is obtained by the measurement of excitation–emission matrices of the endogenous fluorophores and by applying multivariate chemometric tools. Synchronous fluorescence spectroscopy is an advantageous screening mode used in food analysis. The fluorescent markers in food are amino acids tryptophan and tyrosine; the structural proteins collagen and elastin; the enzymes and co-enzymes NADH and FAD; vitamins; lipids; porphyrins; and mycotoxins in certain food types. The review provides information on the principles of the fluorescence measurements of food samples and the advantages of this method over the others. An analysis of the fluorescence spectroscopy applications in screening the various food types is provided.",
publisher = "MDPI",
journal = "Foods",
title = "Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach",
pages = "17-1",
number = "16",
volume = "12",
doi = "10.3390/foods12163023"
}

Radotić, K., Stanković, M., Bartolić, D.,& Natić, M.. (2023). Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach. in Foods
MDPI., 12(16), 1-17.
https://doi.org/10.3390/foods12163023

Radotić K, Stanković M, Bartolić D, Natić M. Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach. in Foods. 2023;12(16):1-17.
doi:10.3390/foods12163023 .

Radotić, Ksenija, Stanković, Mira, Bartolić, Dragana, Natić, Maja, "Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach" in Foods, 12, no. 16 (2023):1-17,
https://doi.org/10.3390/foods12163023 . .

TY  - CONF
AU  - Prokopijević, Miloš
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2377
AB  - Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed the presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food.
PB  - East Sarajevo, Faculty of Agriculture
C3  - XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina
T1  - CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS
EP  - 214
SP  - 214
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2377
ER  - 

@conference{
author = "Prokopijević, Miloš and Bartolić, Dragana and Stanković, Mira and Radotić, Ksenija",
year = "2023",
abstract = "Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed the presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food.",
publisher = "East Sarajevo, Faculty of Agriculture",
journal = "XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina",
title = "CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS",
pages = "214-214",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2377"
}

Prokopijević, M., Bartolić, D., Stanković, M.,& Radotić, K.. (2023). CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS. in XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina
East Sarajevo, Faculty of Agriculture., 214-214.
https://hdl.handle.net/21.15107/rcub_rimsi_2377

Prokopijević M, Bartolić D, Stanković M, Radotić K. CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS. in XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina. 2023;:214-214.
https://hdl.handle.net/21.15107/rcub_rimsi_2377 .

Prokopijević, Miloš, Bartolić, Dragana, Stanković, Mira, Radotić, Ksenija, "CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS" in XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina (2023):214-214,
https://hdl.handle.net/21.15107/rcub_rimsi_2377 .

TY  - GEN
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Bartolić, Dragana
AU  - Stevanovic, Jevrosima
AU  - Andrić, Filip
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2366
AB  - Honey bees have a very important role in pollinating plants, in addition to honey production. Bee
diseases are one of the reasons of a significant decrease in bee colonies in the world in recent years. Honey samples originating from hives with different infestations with N. ceranae were analyzed. Total phenolic content and total protein content were determined by the Folin-Ciocalteu assay and the Bradford assay, respectively. Fluorescence spectroscopy combined with PARAFAC was used to determine the spectral components originating from proteins (PFSC1) and phenolics (PFSC2) in honey samples, and their ratios were calculated. Phenols and proteins content in the honey samples, obtained by spectrophotometric quantification, decreases with increasing infestation levels in the respective hives infected with N. ceranae. A negative correlation was obtained for the ratio of PARAFAC components PFSC2/PFSC1 and the level of infestation with N. ceranae in honey samples. These results indicate that fluorescence spectroscopy combined with PARAFAC could be used as an optical tool for non-invasive and rapid screening of honey to estimate bee health status.
PB  - University of Belgrade, Technical Faculty in Bor
T2  - EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
T1  - ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING
EP  - 46
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2366
ER  - 

@misc{
author = "Stanković, Mira and Prokopijević, Miloš and Bartolić, Dragana and Stevanovic, Jevrosima and Andrić, Filip and Radotić, Ksenija",
year = "2023",
abstract = "Honey bees have a very important role in pollinating plants, in addition to honey production. Bee
diseases are one of the reasons of a significant decrease in bee colonies in the world in recent years. Honey samples originating from hives with different infestations with N. ceranae were analyzed. Total phenolic content and total protein content were determined by the Folin-Ciocalteu assay and the Bradford assay, respectively. Fluorescence spectroscopy combined with PARAFAC was used to determine the spectral components originating from proteins (PFSC1) and phenolics (PFSC2) in honey samples, and their ratios were calculated. Phenols and proteins content in the honey samples, obtained by spectrophotometric quantification, decreases with increasing infestation levels in the respective hives infected with N. ceranae. A negative correlation was obtained for the ratio of PARAFAC components PFSC2/PFSC1 and the level of infestation with N. ceranae in honey samples. These results indicate that fluorescence spectroscopy combined with PARAFAC could be used as an optical tool for non-invasive and rapid screening of honey to estimate bee health status.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research",
title = "ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING",
pages = "46-40",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2366"
}

Stanković, M., Prokopijević, M., Bartolić, D., Stevanovic, J., Andrić, F.,& Radotić, K.. (2023). ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
University of Belgrade, Technical Faculty in Bor., 40-46.
https://hdl.handle.net/21.15107/rcub_rimsi_2366

Stanković M, Prokopijević M, Bartolić D, Stevanovic J, Andrić F, Radotić K. ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research. 2023;:40-46.
https://hdl.handle.net/21.15107/rcub_rimsi_2366 .

Stanković, Mira, Prokopijević, Miloš, Bartolić, Dragana, Stevanovic, Jevrosima, Andrić, Filip, Radotić, Ksenija, "ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING" in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research (2023):40-46,
https://hdl.handle.net/21.15107/rcub_rimsi_2366 .

TY  - CONF
AU  - Surudžić, Nevena
AU  - Spasojević, Dragica
AU  - Stanković, Mira
AU  - Spasojević, Milica
AU  - Elgahwash, Reyadh Gomah Amar
AU  - Prodanović, Radivoje
AU  - Prodanović, Olivera
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2012
AB  - Natural polymers such as alginate, pectin, chitosan etc. were used as carriers for the immobilization of different types of enzymes. Among investigated enzymes, peroxidases hold a special place. Immobilized enzymes are frequently used in phenol removal reactions. In this research horseradish peroxidase was immobilized within alginate micro-beads. This natural polymer was previously oxidized with sodium periodate and modified with tyramine hydrochloride. Percent of oxidation was varied from 2.5 mol% to 10 mol%, and an increase in specific activity was noticed with increasing the oxidation percent. Immobilized peroxidases showed satisfactory stabilities after 10 days of storage. Phenol concentration in a batch reactor decreased during its oxidation with horseradish peroxidase immobilized on tyramine-alginate hydrogels.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th International Conference Ecological Truth and Environmental Research – EcoTER’23
T1  - Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater
EP  - 271
SP  - 267
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2012
ER  - 

@conference{
author = "Surudžić, Nevena and Spasojević, Dragica and Stanković, Mira and Spasojević, Milica and Elgahwash, Reyadh Gomah Amar and Prodanović, Radivoje and Prodanović, Olivera",
year = "2023",
abstract = "Natural polymers such as alginate, pectin, chitosan etc. were used as carriers for the immobilization of different types of enzymes. Among investigated enzymes, peroxidases hold a special place. Immobilized enzymes are frequently used in phenol removal reactions. In this research horseradish peroxidase was immobilized within alginate micro-beads. This natural polymer was previously oxidized with sodium periodate and modified with tyramine hydrochloride. Percent of oxidation was varied from 2.5 mol% to 10 mol%, and an increase in specific activity was noticed with increasing the oxidation percent. Immobilized peroxidases showed satisfactory stabilities after 10 days of storage. Phenol concentration in a batch reactor decreased during its oxidation with horseradish peroxidase immobilized on tyramine-alginate hydrogels.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th International Conference Ecological Truth and Environmental Research – EcoTER’23",
title = "Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater",
pages = "271-267",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2012"
}

Surudžić, N., Spasojević, D., Stanković, M., Spasojević, M., Elgahwash, R. G. A., Prodanović, R.,& Prodanović, O.. (2023). Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater. in 30th International Conference Ecological Truth and Environmental Research – EcoTER’23
University of Belgrade, Technical Faculty in Bor., 267-271.
https://hdl.handle.net/21.15107/rcub_rimsi_2012

Surudžić N, Spasojević D, Stanković M, Spasojević M, Elgahwash RGA, Prodanović R, Prodanović O. Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater. in 30th International Conference Ecological Truth and Environmental Research – EcoTER’23. 2023;:267-271.
https://hdl.handle.net/21.15107/rcub_rimsi_2012 .

Surudžić, Nevena, Spasojević, Dragica, Stanković, Mira, Spasojević, Milica, Elgahwash, Reyadh Gomah Amar, Prodanović, Radivoje, Prodanović, Olivera, "Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater" in 30th International Conference Ecological Truth and Environmental Research – EcoTER’23 (2023):267-271,
https://hdl.handle.net/21.15107/rcub_rimsi_2012 .

TY  - CONF
AU  - Nikolić, Dušan
AU  - Stanković, Mira
AU  - Cvijanović, Gorčin
AU  - Nikčević, Miroslav
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2659
AB  - Uzorci vode iz reke Pek uzeti su u proleće 2022. godine. Analiza potencijalno toksičnih
elelemata (Hg, As, Zn, Cd, Pb, Ni, Fe, B, Cr, Mn i Cu) izvršena je pomoću optičke
emisione spektroskopije sa induktivno spregnutom plazmom (ICP-OES), a aromatičnih
ugljovodonika, policikličnih aromatičnih ugljovodonika i fenola gasnim hromatografom sa
masenom spektometrijom (GC-MS). Koncentracije Mn prelazile su MDK vrednosti
propisane za vodu. Od organskih jedinjenja, detektovani su samo fluoranten, naftalen i
fenantren. Fluorescentna spektroskopija korišćena je za skrining vode na prisustvo
aromatičnih jedinjenja. Dobijeni rezultati pokazali su da se ova metoda može koristiti kao
brzi alat za skrining kvaliteta vode.
AB  - Water samples from the Pek river were collected in the spring 2022. Analysis of potentially
toxic elements (Hg, As, Zn, Cd, Pb, Ni, Fe, B, Cr, Mn, and Cu) was performed using
inductively-coupled plasma optical emission spectrometry (ICP-OES), and aromatic
hydrocarbons, polycyclic aromatic hydrocarbons, and phenol by gas chromatography-mass
spectrometry (GC-MS). Mn concentrations exceeded the MAC values prescribed for water.
Of organic compounds, only fluoranthene, naphthalene, and phenanthrene were detected.
Fluorescence spectroscopy was used for water screening on presence of aromatic
compounds. The results indicated that this method could be used as a fast screening tool for
water quality analysis.
PB  - Српско друштво за заштиту вода, Београд
C3  - Voda 2023 - 52. godišnje konferencije o aktuelnim problemima korišćenja i zaštite voda
T1  - Ispitivanje sadržaja potencijalno toksičnih elemenata i organskih jedinjenja u uzorku vode iz reke Pek (Ujevac, Srbija)
T1  - Investigation on the content of potentially toxic elements and organic compounds in the water sample from the Pek river (Ujevac, Serbia)
EP  - 102
SP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2659
ER  - 

@conference{
author = "Nikolić, Dušan and Stanković, Mira and Cvijanović, Gorčin and Nikčević, Miroslav and Radotić, Ksenija",
year = "2023",
abstract = "Uzorci vode iz reke Pek uzeti su u proleće 2022. godine. Analiza potencijalno toksičnih
elelemata (Hg, As, Zn, Cd, Pb, Ni, Fe, B, Cr, Mn i Cu) izvršena je pomoću optičke
emisione spektroskopije sa induktivno spregnutom plazmom (ICP-OES), a aromatičnih
ugljovodonika, policikličnih aromatičnih ugljovodonika i fenola gasnim hromatografom sa
masenom spektometrijom (GC-MS). Koncentracije Mn prelazile su MDK vrednosti
propisane za vodu. Od organskih jedinjenja, detektovani su samo fluoranten, naftalen i
fenantren. Fluorescentna spektroskopija korišćena je za skrining vode na prisustvo
aromatičnih jedinjenja. Dobijeni rezultati pokazali su da se ova metoda može koristiti kao
brzi alat za skrining kvaliteta vode., Water samples from the Pek river were collected in the spring 2022. Analysis of potentially
toxic elements (Hg, As, Zn, Cd, Pb, Ni, Fe, B, Cr, Mn, and Cu) was performed using
inductively-coupled plasma optical emission spectrometry (ICP-OES), and aromatic
hydrocarbons, polycyclic aromatic hydrocarbons, and phenol by gas chromatography-mass
spectrometry (GC-MS). Mn concentrations exceeded the MAC values prescribed for water.
Of organic compounds, only fluoranthene, naphthalene, and phenanthrene were detected.
Fluorescence spectroscopy was used for water screening on presence of aromatic
compounds. The results indicated that this method could be used as a fast screening tool for
water quality analysis.",
publisher = "Српско друштво за заштиту вода, Београд",
journal = "Voda 2023 - 52. godišnje konferencije o aktuelnim problemima korišćenja i zaštite voda",
title = "Ispitivanje sadržaja potencijalno toksičnih elemenata i organskih jedinjenja u uzorku vode iz reke Pek (Ujevac, Srbija), Investigation on the content of potentially toxic elements and organic compounds in the water sample from the Pek river (Ujevac, Serbia)",
pages = "102-97",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2659"
}

Nikolić, D., Stanković, M., Cvijanović, G., Nikčević, M.,& Radotić, K.. (2023). Ispitivanje sadržaja potencijalno toksičnih elemenata i organskih jedinjenja u uzorku vode iz reke Pek (Ujevac, Srbija). in Voda 2023 - 52. godišnje konferencije o aktuelnim problemima korišćenja i zaštite voda
Српско друштво за заштиту вода, Београд., 97-102.
https://hdl.handle.net/21.15107/rcub_rimsi_2659

Nikolić D, Stanković M, Cvijanović G, Nikčević M, Radotić K. Ispitivanje sadržaja potencijalno toksičnih elemenata i organskih jedinjenja u uzorku vode iz reke Pek (Ujevac, Srbija). in Voda 2023 - 52. godišnje konferencije o aktuelnim problemima korišćenja i zaštite voda. 2023;:97-102.
https://hdl.handle.net/21.15107/rcub_rimsi_2659 .

Nikolić, Dušan, Stanković, Mira, Cvijanović, Gorčin, Nikčević, Miroslav, Radotić, Ksenija, "Ispitivanje sadržaja potencijalno toksičnih elemenata i organskih jedinjenja u uzorku vode iz reke Pek (Ujevac, Srbija)" in Voda 2023 - 52. godišnje konferencije o aktuelnim problemima korišćenja i zaštite voda (2023):97-102,
https://hdl.handle.net/21.15107/rcub_rimsi_2659 .

TY  - CONF
AU  - Prokopijević, Miloš
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Mitrović, Aleksandra Lj.
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1999
AB  - Environmental safety may be compromised by the presence of hazardous chemical compounds, such as bisphenol A (BPA), which is commonly used in the production of certain types of plastics. BPA is an emerging organic contaminant that could be found in many matrices, such as drinking water, due to anthropogenic activities. Within this study, we used fluorescence spectroscopy to analyse the photoluminescent characteristics of BPA in various solutions and drinking water. The emission spectra of BPA in various solvents are recorded from 280 nm to 380 nm, after excitation wavelength at 230 nm. These results imply that the fluorescence approach can be used for rapid detection and estimation of the level of BPA in water samples and, hence, for non-invasive monitoring of BPA in drinking water is an important concern for public health and environmental protection.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
T1  - Fluorescence characterisation of bisphenol A in various solvents and drinking water
EP  - 305
SP  - 302
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1999
ER  - 

@conference{
author = "Prokopijević, Miloš and Stanković, Mira and Bartolić, Dragana and Mitrović, Aleksandra Lj. and Radotić, Ksenija",
year = "2023",
abstract = "Environmental safety may be compromised by the presence of hazardous chemical compounds, such as bisphenol A (BPA), which is commonly used in the production of certain types of plastics. BPA is an emerging organic contaminant that could be found in many matrices, such as drinking water, due to anthropogenic activities. Within this study, we used fluorescence spectroscopy to analyse the photoluminescent characteristics of BPA in various solutions and drinking water. The emission spectra of BPA in various solvents are recorded from 280 nm to 380 nm, after excitation wavelength at 230 nm. These results imply that the fluorescence approach can be used for rapid detection and estimation of the level of BPA in water samples and, hence, for non-invasive monitoring of BPA in drinking water is an important concern for public health and environmental protection.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research",
title = "Fluorescence characterisation of bisphenol A in various solvents and drinking water",
pages = "305-302",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1999"
}

Prokopijević, M., Stanković, M., Bartolić, D., Mitrović, A. Lj.,& Radotić, K.. (2023). Fluorescence characterisation of bisphenol A in various solvents and drinking water. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
University of Belgrade, Technical Faculty in Bor., 302-305.
https://hdl.handle.net/21.15107/rcub_rimsi_1999

Prokopijević M, Stanković M, Bartolić D, Mitrović AL, Radotić K. Fluorescence characterisation of bisphenol A in various solvents and drinking water. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research. 2023;:302-305.
https://hdl.handle.net/21.15107/rcub_rimsi_1999 .

Prokopijević, Miloš, Stanković, Mira, Bartolić, Dragana, Mitrović, Aleksandra Lj., Radotić, Ksenija, "Fluorescence characterisation of bisphenol A in various solvents and drinking water" in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research (2023):302-305,
https://hdl.handle.net/21.15107/rcub_rimsi_1999 .

TY  - GEN
AU  - Bartolić, Dragana
AU  - Lucev, Milica
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Algara, Manuel
AU  - Stanković, Slavica
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2374
AB  - Environmental occurrence of the pathogenic fungus Aspergillus flavus (A. flavus) has hazardous
effects on the health status of plants, humans, and animals. It is important to explore novel antifungal agents to control the growth of this fungus. In this study, we aimed to evaluate the antifungal contact activity of two different types of carbon dots (CDS) nanomaterials; the one was coated with vitamin B12
(CDS@VB12) and the other was obtained from folic acid (FA@CDs). We used fluorescence
spectroscopy to obtain their fluorescence fingerprinting profile. The CDs were pipetted on the PDA
medium with sterilised filter paper placed in the center of the Petri dish. Then, pure A. flavus cultures were subcultured on PDA and incubated at 25°C for 7 days, and the diameters of mycelium growth were daily evaluated. The percentage of inhibition by CDS@VB12 ranged from 38.09% to 60.9% and from 46.03% to 71.39% for the amounts of 30 μl and 50 μl, respectively. On the other hand, FA@CDs did not show any inhibitory effect on the first day, and after that more progressive growth of A. flavus was noticed. The obtained results showed that CDS@VB12 could be considered as a potential new antifungal material against toxic and pathogenic A. flavus.
PB  - University of Belgrade, Technical Faculty in Bor
T2  - EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
T1  - ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus
EP  - 54
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2374
ER  - 

@misc{
author = "Bartolić, Dragana and Lucev, Milica and Stanković, Mira and Prokopijević, Miloš and Algara, Manuel and Stanković, Slavica and Radotić, Ksenija",
year = "2023",
abstract = "Environmental occurrence of the pathogenic fungus Aspergillus flavus (A. flavus) has hazardous
effects on the health status of plants, humans, and animals. It is important to explore novel antifungal agents to control the growth of this fungus. In this study, we aimed to evaluate the antifungal contact activity of two different types of carbon dots (CDS) nanomaterials; the one was coated with vitamin B12
(CDS@VB12) and the other was obtained from folic acid (FA@CDs). We used fluorescence
spectroscopy to obtain their fluorescence fingerprinting profile. The CDs were pipetted on the PDA
medium with sterilised filter paper placed in the center of the Petri dish. Then, pure A. flavus cultures were subcultured on PDA and incubated at 25°C for 7 days, and the diameters of mycelium growth were daily evaluated. The percentage of inhibition by CDS@VB12 ranged from 38.09% to 60.9% and from 46.03% to 71.39% for the amounts of 30 μl and 50 μl, respectively. On the other hand, FA@CDs did not show any inhibitory effect on the first day, and after that more progressive growth of A. flavus was noticed. The obtained results showed that CDS@VB12 could be considered as a potential new antifungal material against toxic and pathogenic A. flavus.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research",
title = "ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus",
pages = "54-47",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2374"
}

Bartolić, D., Lucev, M., Stanković, M., Prokopijević, M., Algara, M., Stanković, S.,& Radotić, K.. (2023). ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
University of Belgrade, Technical Faculty in Bor., 47-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2374

Bartolić D, Lucev M, Stanković M, Prokopijević M, Algara M, Stanković S, Radotić K. ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research. 2023;:47-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2374 .

Bartolić, Dragana, Lucev, Milica, Stanković, Mira, Prokopijević, Miloš, Algara, Manuel, Stanković, Slavica, Radotić, Ksenija, "ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus" in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research (2023):47-54,
https://hdl.handle.net/21.15107/rcub_rimsi_2374 .

TY  - JOUR
AU  - Brdar, Sanja
AU  - Panic, Marko
AU  - Matavulj, Predrag
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Sikoparija, Branko
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1799
AB  - Pollen monitoring have become data-intensive in recent years as real-time detectors are deployed
to classify airborne pollen grains. Machine learning models with a focus on deep learning, have
an essential role in the pollen classifcation task. Within this study we developed an explainable
framework to unveil a deep learning model for pollen classifcation. Model works on data coming
from single particle detector (Rapid-E) that records for each particle optical fngerprint with scattered
light and laser induced fuorescence. Morphological properties of a particle are sensed with the
light scattering process, while chemical properties are encoded with fuorescence spectrum and
fuorescence lifetime induced by high-resolution laser. By utilizing these three data modalities,
scattering, spectrum, and lifetime, deep learning-based models with millions of parameters are
learned to distinguish diferent pollen classes, but a proper understanding of such a black-box model
decisions demands additional methods to employ. Our study provides the frst results of applied
explainable artifcial intelligence (xAI) methodology on the pollen classifcation model. Extracted
knowledge on the important features that attribute to the predicting particular pollen classes is
further examined from the perspective of domain knowledge and compared to available reference
data on pollen sizes, shape, and laboratory spectrofuorometer measurements.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy
IS  - 3205
VL  - 13
DO  - 10.1038/s41598-023-30064-6
ER  - 

@article{
author = "Brdar, Sanja and Panic, Marko and Matavulj, Predrag and Stanković, Mira and Bartolić, Dragana and Sikoparija, Branko",
year = "2023",
abstract = "Pollen monitoring have become data-intensive in recent years as real-time detectors are deployed
to classify airborne pollen grains. Machine learning models with a focus on deep learning, have
an essential role in the pollen classifcation task. Within this study we developed an explainable
framework to unveil a deep learning model for pollen classifcation. Model works on data coming
from single particle detector (Rapid-E) that records for each particle optical fngerprint with scattered
light and laser induced fuorescence. Morphological properties of a particle are sensed with the
light scattering process, while chemical properties are encoded with fuorescence spectrum and
fuorescence lifetime induced by high-resolution laser. By utilizing these three data modalities,
scattering, spectrum, and lifetime, deep learning-based models with millions of parameters are
learned to distinguish diferent pollen classes, but a proper understanding of such a black-box model
decisions demands additional methods to employ. Our study provides the frst results of applied
explainable artifcial intelligence (xAI) methodology on the pollen classifcation model. Extracted
knowledge on the important features that attribute to the predicting particular pollen classes is
further examined from the perspective of domain knowledge and compared to available reference
data on pollen sizes, shape, and laboratory spectrofuorometer measurements.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy",
number = "3205",
volume = "13",
doi = "10.1038/s41598-023-30064-6"
}

Brdar, S., Panic, M., Matavulj, P., Stanković, M., Bartolić, D.,& Sikoparija, B.. (2023). Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy. in Scientific Reports
Springer Nature., 13(3205).
https://doi.org/10.1038/s41598-023-30064-6

Brdar S, Panic M, Matavulj P, Stanković M, Bartolić D, Sikoparija B. Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy. in Scientific Reports. 2023;13(3205).
doi:10.1038/s41598-023-30064-6 .

Brdar, Sanja, Panic, Marko, Matavulj, Predrag, Stanković, Mira, Bartolić, Dragana, Sikoparija, Branko, "Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy" in Scientific Reports, 13, no. 3205 (2023),
https://doi.org/10.1038/s41598-023-30064-6 . .

TY  - JOUR
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Sikoparija, Branko
AU  - Nedic, Nebojsa
AU  - Andric, Filip
AU  - Natalija, Polović
AU  - Natić, Maja
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1781
AB  - Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera)
colonies. This study aimed to find a promising marker in honey for the bee colony infestation level
using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of
the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and
phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level
was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the
infestation level was positively correlated with the phenolic spectral component (r = 0.656), which
was tentatively related to the phenolics in honey. No correlation was found between the diastase
activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in
honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level
of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.
PB  - MDPI
T2  - Foods
T1  - Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies
EP  - 13
SP  - 629
SP  - 1
VL  - 12
DO  - 10.3390/foods12030629
ER  - 

@article{
author = "Stanković, Mira and Prokopijević, Miloš and Sikoparija, Branko and Nedic, Nebojsa and Andric, Filip and Natalija, Polović and Natić, Maja and Radotić, Ksenija",
year = "2023",
abstract = "Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera)
colonies. This study aimed to find a promising marker in honey for the bee colony infestation level
using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of
the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and
phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level
was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the
infestation level was positively correlated with the phenolic spectral component (r = 0.656), which
was tentatively related to the phenolics in honey. No correlation was found between the diastase
activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in
honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level
of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.",
publisher = "MDPI",
journal = "Foods",
title = "Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies",
pages = "13-629-1",
volume = "12",
doi = "10.3390/foods12030629"
}

Stanković, M., Prokopijević, M., Sikoparija, B., Nedic, N., Andric, F., Natalija, P., Natić, M.,& Radotić, K.. (2023). Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods
MDPI., 12, 629-13.
https://doi.org/10.3390/foods12030629

Stanković M, Prokopijević M, Sikoparija B, Nedic N, Andric F, Natalija P, Natić M, Radotić K. Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods. 2023;12:629-13.
doi:10.3390/foods12030629 .

Stanković, Mira, Prokopijević, Miloš, Sikoparija, Branko, Nedic, Nebojsa, Andric, Filip, Natalija, Polović, Natić, Maja, Radotić, Ksenija, "Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies" in Foods, 12 (2023):629-13,
https://doi.org/10.3390/foods12030629 . .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Pantić, Nevena
AU  - Stanković, Mira
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1767
AB  - Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 29th International Conference Ecological Truth and Environmental Research - EcoTER'22
T1  - PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP
EP  - 363
SP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1767
ER  - 

@conference{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Pantić, Nevena and Stanković, Mira and Radotić, Ksenija and Prodanović, Radivoje",
year = "2022",
abstract = "Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "29th International Conference Ecological Truth and Environmental Research - EcoTER'22",
title = "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP",
pages = "363-360",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1767"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Pantić, N., Stanković, M., Radotić, K.,& Prodanović, R.. (2022). PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22
University of Belgrade, Technical Faculty in Bor., 360-363.
https://hdl.handle.net/21.15107/rcub_rimsi_1767

Spasojević D, Prokopijević M, Prodanović O, Pantić N, Stanković M, Radotić K, Prodanović R. PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22. 2022;:360-363.
https://hdl.handle.net/21.15107/rcub_rimsi_1767 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Pantić, Nevena, Stanković, Mira, Radotić, Ksenija, Prodanović, Radivoje, "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP" in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22 (2022):360-363,
https://hdl.handle.net/21.15107/rcub_rimsi_1767 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1780
AB  - Semena kukuruza (Zea mais L.) mogu biti kontaminirana sa jednim od najpasnijih prirodnih kancerogenih kontaminanata kao što su aflatoksini. U ovoj studiji, proučavan je uticaj aflateritoksina na strukturne karakteristike strukture. Korišćena je fluorescentna spektropija u kombinaciji sa matematičkom analizom spektra. Primenjen je matematički model za analizu spektra asimetričnog modela (Log-normal) koji koristi Nelder-Mead algoritam (Matlab 6.5). Rezultati razlaganja fluorescentnih spektara pokazuju četiri glavne fluorescentne fluorescentne emisije sa 054 emisiona približna 050 komponenta. Ovaj rezultat može da se koristi za praćenje promene u semenu usled prisustva aflatoksina. Glavni pokazatelj uticaja aflatoksina na strukturu kukuružnog semena je pomeraj dugo-talasne emisione komponente (520 nm).
PB  - Srpsko biološko društvo, Beograd, 2022.
C3  - Treći kongres biologa Srbije
T1  - Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom
SP  - 21
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1780
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2022",
abstract = "Semena kukuruza (Zea mais L.) mogu biti kontaminirana sa jednim od najpasnijih prirodnih kancerogenih kontaminanata kao što su aflatoksini. U ovoj studiji, proučavan je uticaj aflateritoksina na strukturne karakteristike strukture. Korišćena je fluorescentna spektropija u kombinaciji sa matematičkom analizom spektra. Primenjen je matematički model za analizu spektra asimetričnog modela (Log-normal) koji koristi Nelder-Mead algoritam (Matlab 6.5). Rezultati razlaganja fluorescentnih spektara pokazuju četiri glavne fluorescentne fluorescentne emisije sa 054 emisiona približna 050 komponenta. Ovaj rezultat može da se koristi za praćenje promene u semenu usled prisustva aflatoksina. Glavni pokazatelj uticaja aflatoksina na strukturu kukuružnog semena je pomeraj dugo-talasne emisione komponente (520 nm).",
publisher = "Srpsko biološko društvo, Beograd, 2022.",
journal = "Treći kongres biologa Srbije",
title = "Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom",
pages = "21",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1780"
}

Bartolić, D., Stanković, M., Prokopijević, M., Đikanović, D., Kalauzi, A.,& Radotić, K.. (2022). Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom. in Treći kongres biologa Srbije
Srpsko biološko društvo, Beograd, 2022.., 21.
https://hdl.handle.net/21.15107/rcub_rimsi_1780

Bartolić D, Stanković M, Prokopijević M, Đikanović D, Kalauzi A, Radotić K. Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom. in Treći kongres biologa Srbije. 2022;:21.
https://hdl.handle.net/21.15107/rcub_rimsi_1780 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Radotić, Ksenija, "Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom" in Treći kongres biologa Srbije (2022):21,
https://hdl.handle.net/21.15107/rcub_rimsi_1780 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1699
AB  - Varroa infestation is considered a major threat to honeybees due to its strong impact on the colony, attacking larval cells, increasing bee mortality, and serving as a vector of pathogens. Coumaphos is an organophosphate-based acaricide insecticide which is frequently used to control Varroa (Varroa destructor) mites in the honey bee (Apis melifera) colonies. The accumulation of these lipophilic contaminants in honey and beeswax may affect honey bee health, mainly honey bee larvae which are the most sensitive, and negatively affect queen quality. In this study, we analyzed honey samples from hives of honey bees that were treated with Coumaphos, as well as the samples of pure Coumafos. The front-face fluorescence measurements of analyed honey samples were recorded using an Fl3-221 P spectrofluorimeter, with a 450 W Xenon lamp. Obtained results showed an emission peak with maximum around 400 nm, after excitation at 340 nm. It could be related to the presence of Coumaphos in honey samples, which showed the characteristic position of emission maximum at the same wavelength. These results showed that fluorescence spectroscopy may be useful as a fast and sensitive method, for the efficient and specific determination of toxic substances such as Coumaphos residues in honey. Due to the proven toxic effects on honey bees, as well as on humans, monitoring hazardous contaminants in honey is of crucial importance in the field of food and agriculture.
PB  - East Sarajevo : Faculty of Agriculture, 2022
C3  - XIII International Scientific Agriculture Symposium “AGROSYM 2022”
T1  - DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY
SP  - 342
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1699
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Đikanović, Daniela and Radotić, Ksenija",
year = "2022",
abstract = "Varroa infestation is considered a major threat to honeybees due to its strong impact on the colony, attacking larval cells, increasing bee mortality, and serving as a vector of pathogens. Coumaphos is an organophosphate-based acaricide insecticide which is frequently used to control Varroa (Varroa destructor) mites in the honey bee (Apis melifera) colonies. The accumulation of these lipophilic contaminants in honey and beeswax may affect honey bee health, mainly honey bee larvae which are the most sensitive, and negatively affect queen quality. In this study, we analyzed honey samples from hives of honey bees that were treated with Coumaphos, as well as the samples of pure Coumafos. The front-face fluorescence measurements of analyed honey samples were recorded using an Fl3-221 P spectrofluorimeter, with a 450 W Xenon lamp. Obtained results showed an emission peak with maximum around 400 nm, after excitation at 340 nm. It could be related to the presence of Coumaphos in honey samples, which showed the characteristic position of emission maximum at the same wavelength. These results showed that fluorescence spectroscopy may be useful as a fast and sensitive method, for the efficient and specific determination of toxic substances such as Coumaphos residues in honey. Due to the proven toxic effects on honey bees, as well as on humans, monitoring hazardous contaminants in honey is of crucial importance in the field of food and agriculture.",
publisher = "East Sarajevo : Faculty of Agriculture, 2022",
journal = "XIII International Scientific Agriculture Symposium “AGROSYM 2022”",
title = "DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY",
pages = "342",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1699"
}

Bartolić, D., Stanković, M., Prokopijević, M., Đikanović, D.,& Radotić, K.. (2022). DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”
East Sarajevo : Faculty of Agriculture, 2022., 342.
https://hdl.handle.net/21.15107/rcub_rimsi_1699

Bartolić D, Stanković M, Prokopijević M, Đikanović D, Radotić K. DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”. 2022;:342.
https://hdl.handle.net/21.15107/rcub_rimsi_1699 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Đikanović, Daniela, Radotić, Ksenija, "DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY" in XIII International Scientific Agriculture Symposium “AGROSYM 2022” (2022):342,
https://hdl.handle.net/21.15107/rcub_rimsi_1699 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Đikanović, Daniela
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Jovanović, Jelena
AU  - Dragišić Maksimović, Jelena
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1704
AB  - Silica is beneficial element for plants. Many studies of silica show the useful effects that Si can provide on plant-environment relationships in a wide variety of crops, as increase in growth and yield and improvement of resistance to metal toxicity, salt stress, drought resistance etc. The occurrence of Si within a plant is a result of its uptake in the form of soluble Si(OH)4 or Si(OH)3O ion from the soil, and its polymerization at a final location. Si is mostly accumulated in the cell walls (CWs), thus providing strengthening of CWs, increasing mechanical strength, and alleviating effects of various biotic and abiotic types of stress. Lignin, as one of the main components of the plant CW, is a natural phenolic polymer with high molecular weight and complex structure. Accumulation of silica in plants is directly connected with chemistry and structure of lignin. Silicon affects the composition of CWs by its binding to the phenolic and carbohydrate CW components in polymerization reactions by altering linkages of non-cellulosic polymers and lignin. We studied, in an in vitro system, the interaction of SiO2 with the peroxidase catalyzed polymerization of a lignin monomer into the lignin model compound (DHP). Fluorescence microscopy and spectroscopy combined with AFM technique showed that Si was bound to the final polymer, and the structure of the Si-DHP differed from pure DHP. We show that Si significantly influence on lignin structure. The results contribute to understanding of Si binding to lignin in cell walls, which may have impact on possible Si applications in agriculture.
PB  - East Sarajevo : Faculty of Agriculture, 2022
C3  - XIII International Scientific Agriculture Symposium “AGROSYM 2022”
T1  - INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE
SP  - 341
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1704
ER  - 

@conference{
author = "Bartolić, Dragana and Đikanović, Daniela and Stanković, Mira and Prokopijević, Miloš and Jovanović, Jelena and Dragišić Maksimović, Jelena and Radotić, Ksenija",
year = "2022",
abstract = "Silica is beneficial element for plants. Many studies of silica show the useful effects that Si can provide on plant-environment relationships in a wide variety of crops, as increase in growth and yield and improvement of resistance to metal toxicity, salt stress, drought resistance etc. The occurrence of Si within a plant is a result of its uptake in the form of soluble Si(OH)4 or Si(OH)3O ion from the soil, and its polymerization at a final location. Si is mostly accumulated in the cell walls (CWs), thus providing strengthening of CWs, increasing mechanical strength, and alleviating effects of various biotic and abiotic types of stress. Lignin, as one of the main components of the plant CW, is a natural phenolic polymer with high molecular weight and complex structure. Accumulation of silica in plants is directly connected with chemistry and structure of lignin. Silicon affects the composition of CWs by its binding to the phenolic and carbohydrate CW components in polymerization reactions by altering linkages of non-cellulosic polymers and lignin. We studied, in an in vitro system, the interaction of SiO2 with the peroxidase catalyzed polymerization of a lignin monomer into the lignin model compound (DHP). Fluorescence microscopy and spectroscopy combined with AFM technique showed that Si was bound to the final polymer, and the structure of the Si-DHP differed from pure DHP. We show that Si significantly influence on lignin structure. The results contribute to understanding of Si binding to lignin in cell walls, which may have impact on possible Si applications in agriculture.",
publisher = "East Sarajevo : Faculty of Agriculture, 2022",
journal = "XIII International Scientific Agriculture Symposium “AGROSYM 2022”",
title = "INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE",
pages = "341",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1704"
}

Bartolić, D., Đikanović, D., Stanković, M., Prokopijević, M., Jovanović, J., Dragišić Maksimović, J.,& Radotić, K.. (2022). INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”
East Sarajevo : Faculty of Agriculture, 2022., 341.
https://hdl.handle.net/21.15107/rcub_rimsi_1704

Bartolić D, Đikanović D, Stanković M, Prokopijević M, Jovanović J, Dragišić Maksimović J, Radotić K. INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”. 2022;:341.
https://hdl.handle.net/21.15107/rcub_rimsi_1704 .

Bartolić, Dragana, Đikanović, Daniela, Stanković, Mira, Prokopijević, Miloš, Jovanović, Jelena, Dragišić Maksimović, Jelena, Radotić, Ksenija, "INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE" in XIII International Scientific Agriculture Symposium “AGROSYM 2022” (2022):341,
https://hdl.handle.net/21.15107/rcub_rimsi_1704 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1700
AB  - Mung bean (Vigna Radiata L.) seeds are an important source of both nutrients (such as proteins, fibers, vitamins) and a variety of bioactive compounds (like phenolic compounds). Ultraviolet (UV) light has an important function as a major environmental signal important for plant growth and development, but at the same time it may cause certain damaging effects on macromolecules and other cellular components. Plants respond to the production of reactive oxygen species (ROS), caused by UV irradiation, by activating changes in morphology, physiology, or production of secondary metabolites. Some of these mechanisms increase antioxidant capacity in order to reduce the harmful effect of produced ROS. In the present research, the duration of exposure to UV-A irradiation and its influence on antioxidant activity of mung bean seeds were studied. Seeds were exposed to constant irradiation for 1 or 3 hours using a UV-A lamp (93μW/cm2) at a 50 cm distance. Antioxidant activity was tested using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. The results indicate a significant increase in antioxidant activity of the UV-A irradiated seeds after 1 hour (74,45 % ± 0,40), compared to the control (72,85 % ± 1,55). After prolonged exposure, measured antioxidant activity significantly increased (77,99 % ± 0,71). Our results show a correlation between duration of UV-A irradiation and the increase in antioxidant activity. This could be beneficial in agriculture for producing fortified food.
PB  - East Sarajevo : Faculty of Agriculture, 2022
C3  - XIII International Scientific Agriculture Symposium “AGROSYM 2022”
T1  - BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS
SP  - 203
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1700
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Đikanović, Daniela and Radotić, Ksenija",
year = "2022",
abstract = "Mung bean (Vigna Radiata L.) seeds are an important source of both nutrients (such as proteins, fibers, vitamins) and a variety of bioactive compounds (like phenolic compounds). Ultraviolet (UV) light has an important function as a major environmental signal important for plant growth and development, but at the same time it may cause certain damaging effects on macromolecules and other cellular components. Plants respond to the production of reactive oxygen species (ROS), caused by UV irradiation, by activating changes in morphology, physiology, or production of secondary metabolites. Some of these mechanisms increase antioxidant capacity in order to reduce the harmful effect of produced ROS. In the present research, the duration of exposure to UV-A irradiation and its influence on antioxidant activity of mung bean seeds were studied. Seeds were exposed to constant irradiation for 1 or 3 hours using a UV-A lamp (93μW/cm2) at a 50 cm distance. Antioxidant activity was tested using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. The results indicate a significant increase in antioxidant activity of the UV-A irradiated seeds after 1 hour (74,45 % ± 0,40), compared to the control (72,85 % ± 1,55). After prolonged exposure, measured antioxidant activity significantly increased (77,99 % ± 0,71). Our results show a correlation between duration of UV-A irradiation and the increase in antioxidant activity. This could be beneficial in agriculture for producing fortified food.",
publisher = "East Sarajevo : Faculty of Agriculture, 2022",
journal = "XIII International Scientific Agriculture Symposium “AGROSYM 2022”",
title = "BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS",
pages = "203",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1700"
}

Bartolić, D., Stanković, M., Prokopijević, M., Đikanović, D.,& Radotić, K.. (2022). BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”
East Sarajevo : Faculty of Agriculture, 2022., 203.
https://hdl.handle.net/21.15107/rcub_rimsi_1700

Bartolić D, Stanković M, Prokopijević M, Đikanović D, Radotić K. BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”. 2022;:203.
https://hdl.handle.net/21.15107/rcub_rimsi_1700 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Đikanović, Daniela, Radotić, Ksenija, "BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS" in XIII International Scientific Agriculture Symposium “AGROSYM 2022” (2022):203,
https://hdl.handle.net/21.15107/rcub_rimsi_1700 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Prokopijević, Miloš
AU  - Stanković, Mira
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1765
AB  - Mung bean (Vigna radiata L.) is a leguminous plant with high nutritional value, traditionally known as a functional food. Legume seeds are a rich source of proteins, vitamins, minerals, and essential amino acids but also contain bioactive components and polyphenols which possess a high antioxidant capacity. Pigments content (chlorophyll a and b, carotenoids) was determined as good parameter for estimation of seed quality and an indicator of tolerance to different types of stress. The antioxidant activity of the seeds was determined using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. The concentration of chlorophile a and b and carotenoids were determined by a spectrophotometric method. Obtained results indicate a higher content of chlorophyll a than chlorophyll b, 0.352 μg/ml and 0.220 μg/ml respectively, while total carotenoids content was 0.108 μg/ml and DPPH radical scavenging activity was 54.52% ± 1.77. The advancement in this research lies in collecting information about bioactive compounds, such as chlorophylls and carotenoids, that are useful in improving the functional and antioxidant properties of quality seeds used in daily diet.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš; Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022
T1  - Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content
SP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1765
ER  - 

@conference{
author = "Bartolić, Dragana and Prokopijević, Miloš and Stanković, Mira and Radotić, Ksenija",
year = "2022",
abstract = "Mung bean (Vigna radiata L.) is a leguminous plant with high nutritional value, traditionally known as a functional food. Legume seeds are a rich source of proteins, vitamins, minerals, and essential amino acids but also contain bioactive components and polyphenols which possess a high antioxidant capacity. Pigments content (chlorophyll a and b, carotenoids) was determined as good parameter for estimation of seed quality and an indicator of tolerance to different types of stress. The antioxidant activity of the seeds was determined using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. The concentration of chlorophile a and b and carotenoids were determined by a spectrophotometric method. Obtained results indicate a higher content of chlorophyll a than chlorophyll b, 0.352 μg/ml and 0.220 μg/ml respectively, while total carotenoids content was 0.108 μg/ml and DPPH radical scavenging activity was 54.52% ± 1.77. The advancement in this research lies in collecting information about bioactive compounds, such as chlorophylls and carotenoids, that are useful in improving the functional and antioxidant properties of quality seeds used in daily diet.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš; Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022",
title = "Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content",
pages = "202",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1765"
}

Bartolić, D., Prokopijević, M., Stanković, M.,& Radotić, K.. (2022). Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš; Institute for Nature Conservation of Serbia, Belgrade., 202.
https://hdl.handle.net/21.15107/rcub_rimsi_1765

Bartolić D, Prokopijević M, Stanković M, Radotić K. Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022. 2022;:202.
https://hdl.handle.net/21.15107/rcub_rimsi_1765 .

Bartolić, Dragana, Prokopijević, Miloš, Stanković, Mira, Radotić, Ksenija, "Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022 (2022):202,
https://hdl.handle.net/21.15107/rcub_rimsi_1765 .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1772
AB  - Plant secondary metabolism, as well as the growth and development of specific photomorphogenic
responses, can be induced by UV light. The effects of UV-A and UV-B irradiations on the antioxidant activity and fluorescence characteristics of Glycine max L. seeds were investigated for two different exposure periods (1, 3 h). The antioxidant activity and photoluminescent characteristics of irradiated seeds were evaluated by the DPPH (2,2'-diphenyl-1-picrylhydrazyl radical) assay and excitation-emission matrices (EEMs) fluorescence spectroscopy, respectively. The DPPH scavenging activity significantly increased with exposure time in the UV-A treated seeds, while variations were observed after UV-B treatment. EEM showed three main peaks characterized with excitation/emission 280/355 nm (Peak A), 290/400 nm (Peak B), and 370/465 nm (Peak C). The most prominent change in fluorescence intensity was observed in peak B. Obtained results indicate that the type of UV radiation, as well as period of exposure, induce biochemical and biophysical changes in the soybean seeds.
PB  - Pleiades Publishing, Ltd.
T2  - Russian Journal of Physical Chemistry A
T1  - Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds
EP  - 2800
IS  - 12
SP  - 2797
VL  - 96
DO  - 10.1134/S0036024422120044
ER  - 

@article{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Radotić, Ksenija",
year = "2022",
abstract = "Plant secondary metabolism, as well as the growth and development of specific photomorphogenic
responses, can be induced by UV light. The effects of UV-A and UV-B irradiations on the antioxidant activity and fluorescence characteristics of Glycine max L. seeds were investigated for two different exposure periods (1, 3 h). The antioxidant activity and photoluminescent characteristics of irradiated seeds were evaluated by the DPPH (2,2'-diphenyl-1-picrylhydrazyl radical) assay and excitation-emission matrices (EEMs) fluorescence spectroscopy, respectively. The DPPH scavenging activity significantly increased with exposure time in the UV-A treated seeds, while variations were observed after UV-B treatment. EEM showed three main peaks characterized with excitation/emission 280/355 nm (Peak A), 290/400 nm (Peak B), and 370/465 nm (Peak C). The most prominent change in fluorescence intensity was observed in peak B. Obtained results indicate that the type of UV radiation, as well as period of exposure, induce biochemical and biophysical changes in the soybean seeds.",
publisher = "Pleiades Publishing, Ltd.",
journal = "Russian Journal of Physical Chemistry A",
title = "Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds",
pages = "2800-2797",
number = "12",
volume = "96",
doi = "10.1134/S0036024422120044"
}

Bartolić, D., Stanković, M., Prokopijević, M.,& Radotić, K.. (2022). Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds. in Russian Journal of Physical Chemistry A
Pleiades Publishing, Ltd.., 96(12), 2797-2800.
https://doi.org/10.1134/S0036024422120044

Bartolić D, Stanković M, Prokopijević M, Radotić K. Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds. in Russian Journal of Physical Chemistry A. 2022;96(12):2797-2800.
doi:10.1134/S0036024422120044 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Radotić, Ksenija, "Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds" in Russian Journal of Physical Chemistry A, 96, no. 12 (2022):2797-2800,
https://doi.org/10.1134/S0036024422120044 . .

TY  - JOUR
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Mutavdžić, Dragosav
AU  - Marković, Smilja
AU  - Grubic, Sasa
AU  - Jovanović, Nemanja M.
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1550
AB  - In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R (2) was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-ALS could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples
DO  - 10.1080/00218839.2021.1889803
ER  - 

@article{
author = "Stanković, Mira and Bartolić, Dragana and Mutavdžić, Dragosav and Marković, Smilja and Grubic, Sasa and Jovanović, Nemanja M. and Radotić, Ksenija",
year = "2022",
abstract = "In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R (2) was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-ALS could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples",
doi = "10.1080/00218839.2021.1889803"
}

Stanković, M., Bartolić, D., Mutavdžić, D., Marković, S., Grubic, S., Jovanović, N. M.,& Radotić, K.. (2022). Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon..
https://doi.org/10.1080/00218839.2021.1889803

Stanković M, Bartolić D, Mutavdžić D, Marković S, Grubic S, Jovanović NM, Radotić K. Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples. in Journal of Apicultural Research. 2022;.
doi:10.1080/00218839.2021.1889803 .

Stanković, Mira, Bartolić, Dragana, Mutavdžić, Dragosav, Marković, Smilja, Grubic, Sasa, Jovanović, Nemanja M., Radotić, Ksenija, "Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples" in Journal of Apicultural Research (2022),
https://doi.org/10.1080/00218839.2021.1889803 . .

TY  - THES
AU  - Stanković, Mira
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1834
AB  - Predmet rada ove doktorske disertacije je razvoj analitičkih postupaka za praćenje relativnog sadržajaukupnih proteina i ukupnih fenola u različitim uzorcima meda radi procene uticaja biotičkog stresa napčelinja društva usled zaraze parazitima Varroa destructor i Nosema ceranae.Pored proizvodnje meda, pčele imaju veoma važnu ulogu u oprašivanju biljaka. Poslednjihgodina u svetu dolazi do znatnog smanjenja pčelinjih društava, a jedan od razloga zbog kog dolazi doove pojave su bolesti pčela. U literaturi je malo podataka o kvalitetu meda koji potiče iz pčelinjihzajednica izloženih biotičkom stresu.U cilju nalaženja indikatora u medu za procenu stepena zaraženosti pčelinjih društava,prikupljeni su uzorci meda iz košnica zaraženih varoom i nozemom, a zatim analizirani odabranimbiohemijskim i fizičkohemijskim metodama. Određena je vrsta i stepen infekcije košnica pčelinjihdruštava iz kojih su prikupljeni uzorci meda. Polenskom analizom je određena botanička vrsta meda inije utvrđena direktna veza između prisustva i koncentracije polena u uzorcima meda i stepenazaraženosti košnica. Uzorci meda su okarakterisani fizičkohemijskim metodama. Određeni suspecifična optička rotacija, električna provodljivost, sadržaj vlage, slobodna kiselost i sadržajpojedinačnih šećera. Profil šećera u uzorcima meda određen je visokoefikasnom jonskomhromatografijom sa elektrohemijskom detekcijom (HPAEC-PAD). Analiza glavnih komponenata(PCA) je upotreblјena za utvrđivanje razlika između medova koji potiču iz košnica različitog stepenazaraženosti na osnovu dobijenih fizičkohemijskih parametara. Rezultati analize glavnih komponenatasu pokazali da sa porastom infekcije košnica u uzorcima meda raste sadržaj vode dok slobodna kiselost,apsolutna vrednost specifične optičke rotacije i električna provodljivost opadaju. Veći sadržaj fruktoze,melbioze i melezitoze izmeren je u uzorcima meda iz košnica višeg stepena infekcije u odnosu nauzorke meda koji potiču iz košnica manje zaraženosti. Sadržaj glukoze i izomaltoze bio je veći uuzorcima meda koji potiču od pčelinjih društava manje zaraženosti u odnosu na uzorke meda iz košnicaveće zaraženosti.U cilju ispitivanja varijacije sadržaja biljnih i pčelinjih proteina u medu primenjene suelektroforetska analiza i određivanje aktivnosti enzima. Pomoću natrijum dodecilsulfat poliakrilamidgel elektroforeze (SDS PAGE) nije utvrđena značajna razlika u proteinskom profilu analiziranihuzoraka. Stoga je primenjena spektrofotometrijska metoda određivanja aktivnosti specifičnih enzima,katalaze i dijastaze, kao biljnog odnosno pčelinjeg marker enzima. Rezultati korelacione analize supokazali da je stepen zaraze košnica u pozitivnoj korelaciji sa aktivnošću katalaze i može bitipotencijalno koristan indikator za skrining meda poreklom iz košnica zaraženih varoom i nozemom.Pored toga, nije utvrđena promena u aktivnosti dijastaze u uzorcima meda čije je poreklo od društavakoja su zaražena.Fluorescentna spektroskopija u kombinaciji sa naprednim statističkim metodamamultivarijaciona rezolucija krivih – naizmenični najmanji kvadrati (MCR-ALS) i paralelna faktorskaanaliza (PARAFAC) je korišćena za određivanje relativnog sadržaja proteina i fenola u uzorcima meda...
AB  - Protein and phenol content in honey samples as indicators of theinfluence of stress factors on honey bee coloniesAbstractThe subject of this doctoral dissertation is the development of analytical procedures for monitoring therelative content of total proteins and total phenols in different honey samples to assess the impact ofbiotic stress on honey bee colonies due infected with parasites Varroa destructor and Nosema ceranae.In addition to honey production, bees have very important role in pollinating plants. Inrecent years, there has been a significant decrease in bee colonies in the world and one of the reasonswhy this phenomenon occurs is bee diseases. In the literature, the data are not abundant on the honeyquality change related to bee colonies exposure to biotic stress.In order to find markers in honey to assess the degree of infection of bee colonies, honeysamples were collected from hives infected with parasites V. destructor and N. ceranae parasites andanalyzed by selected biochemical and physicochemical methods. The type and degree of infection ofbee colonies related to the analyzed honey samples were determined. Pollen analysis has proved thebotanical type of honey samples and it was observed that the presence and concentration of pollen inhoney samples were not correlated with the infestation of hives. Honey samples were characterized byphysicochemical methods. Specific optical rotation, electrical conductivity, moisture content, freeacidity, and sugar content were determined to obtain additional characteristics of honey samples. Sugarprofile in honey samples was determined by high performance anion exchange chromatography withpulsed amperometric detection (HPAEC-PAD). Principal component analysis (PCA) was used todetermine differences between honey samples originating from the hives with different infection levels.The obtained results showed that in hives with the increase of infection, in honey samples the watercontent increased, while the free acidity, the absolute value of the specific optical rotation, and theelectrical conductivity decreased. The higher content of fructose, melbiosis and melezitose wasobserved in honey samples from the hives with a higher degree of infection in comparison with honeysamples from the hives with the lower infection degree. Glucose and isomaltose content was higher inhoney samples originating from the bee colonies less infected compared to honey samples from thehives with the higher infection.In order to investigate variations in the content of plant and bee protein markers in honey,biochemical methods such as electrophoretic analysis and determination of enzyme activity wereapplied. Using sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS PAGE), no significantdifference was found in the protein profile of the analyzed honey samples. Therefore, thespectrophotometric method was applied for determination of the activity of specific enzymes, catalaseand diastase, as plant and bee marker enzymes, respectively. The results of correlation analysis showedthat the colonies’ infestation level was positively correlated with the activity of catalase which can be auseful indicator for screening of honey originating from beehives infested by V. destructor and N.ceranae. On the other hand, no change in diastase activity was found in honey samples obtained frominfected societies.Fluorescence spectroscopy in combination with advanced statistical methods MultivariateCurve Resolution - Alternating Least Squares (MCR-ALS) and Parallel Factor Analysis (PARAFAC)was used to determine the relative protein and phenol content in honey samples...
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Određivanje sadržaja proteina i fenola u uzorcima meda kao indikatora uticaja faktora stresa na pčelinja društva
UR  - https://hdl.handle.net/21.15107/rcub_nardus_21242
ER  - 

@phdthesis{
author = "Stanković, Mira",
year = "2022",
abstract = "Predmet rada ove doktorske disertacije je razvoj analitičkih postupaka za praćenje relativnog sadržajaukupnih proteina i ukupnih fenola u različitim uzorcima meda radi procene uticaja biotičkog stresa napčelinja društva usled zaraze parazitima Varroa destructor i Nosema ceranae.Pored proizvodnje meda, pčele imaju veoma važnu ulogu u oprašivanju biljaka. Poslednjihgodina u svetu dolazi do znatnog smanjenja pčelinjih društava, a jedan od razloga zbog kog dolazi doove pojave su bolesti pčela. U literaturi je malo podataka o kvalitetu meda koji potiče iz pčelinjihzajednica izloženih biotičkom stresu.U cilju nalaženja indikatora u medu za procenu stepena zaraženosti pčelinjih društava,prikupljeni su uzorci meda iz košnica zaraženih varoom i nozemom, a zatim analizirani odabranimbiohemijskim i fizičkohemijskim metodama. Određena je vrsta i stepen infekcije košnica pčelinjihdruštava iz kojih su prikupljeni uzorci meda. Polenskom analizom je određena botanička vrsta meda inije utvrđena direktna veza između prisustva i koncentracije polena u uzorcima meda i stepenazaraženosti košnica. Uzorci meda su okarakterisani fizičkohemijskim metodama. Određeni suspecifična optička rotacija, električna provodljivost, sadržaj vlage, slobodna kiselost i sadržajpojedinačnih šećera. Profil šećera u uzorcima meda određen je visokoefikasnom jonskomhromatografijom sa elektrohemijskom detekcijom (HPAEC-PAD). Analiza glavnih komponenata(PCA) je upotreblјena za utvrđivanje razlika između medova koji potiču iz košnica različitog stepenazaraženosti na osnovu dobijenih fizičkohemijskih parametara. Rezultati analize glavnih komponenatasu pokazali da sa porastom infekcije košnica u uzorcima meda raste sadržaj vode dok slobodna kiselost,apsolutna vrednost specifične optičke rotacije i električna provodljivost opadaju. Veći sadržaj fruktoze,melbioze i melezitoze izmeren je u uzorcima meda iz košnica višeg stepena infekcije u odnosu nauzorke meda koji potiču iz košnica manje zaraženosti. Sadržaj glukoze i izomaltoze bio je veći uuzorcima meda koji potiču od pčelinjih društava manje zaraženosti u odnosu na uzorke meda iz košnicaveće zaraženosti.U cilju ispitivanja varijacije sadržaja biljnih i pčelinjih proteina u medu primenjene suelektroforetska analiza i određivanje aktivnosti enzima. Pomoću natrijum dodecilsulfat poliakrilamidgel elektroforeze (SDS PAGE) nije utvrđena značajna razlika u proteinskom profilu analiziranihuzoraka. Stoga je primenjena spektrofotometrijska metoda određivanja aktivnosti specifičnih enzima,katalaze i dijastaze, kao biljnog odnosno pčelinjeg marker enzima. Rezultati korelacione analize supokazali da je stepen zaraze košnica u pozitivnoj korelaciji sa aktivnošću katalaze i može bitipotencijalno koristan indikator za skrining meda poreklom iz košnica zaraženih varoom i nozemom.Pored toga, nije utvrđena promena u aktivnosti dijastaze u uzorcima meda čije je poreklo od društavakoja su zaražena.Fluorescentna spektroskopija u kombinaciji sa naprednim statističkim metodamamultivarijaciona rezolucija krivih – naizmenični najmanji kvadrati (MCR-ALS) i paralelna faktorskaanaliza (PARAFAC) je korišćena za određivanje relativnog sadržaja proteina i fenola u uzorcima meda..., Protein and phenol content in honey samples as indicators of theinfluence of stress factors on honey bee coloniesAbstractThe subject of this doctoral dissertation is the development of analytical procedures for monitoring therelative content of total proteins and total phenols in different honey samples to assess the impact ofbiotic stress on honey bee colonies due infected with parasites Varroa destructor and Nosema ceranae.In addition to honey production, bees have very important role in pollinating plants. Inrecent years, there has been a significant decrease in bee colonies in the world and one of the reasonswhy this phenomenon occurs is bee diseases. In the literature, the data are not abundant on the honeyquality change related to bee colonies exposure to biotic stress.In order to find markers in honey to assess the degree of infection of bee colonies, honeysamples were collected from hives infected with parasites V. destructor and N. ceranae parasites andanalyzed by selected biochemical and physicochemical methods. The type and degree of infection ofbee colonies related to the analyzed honey samples were determined. Pollen analysis has proved thebotanical type of honey samples and it was observed that the presence and concentration of pollen inhoney samples were not correlated with the infestation of hives. Honey samples were characterized byphysicochemical methods. Specific optical rotation, electrical conductivity, moisture content, freeacidity, and sugar content were determined to obtain additional characteristics of honey samples. Sugarprofile in honey samples was determined by high performance anion exchange chromatography withpulsed amperometric detection (HPAEC-PAD). Principal component analysis (PCA) was used todetermine differences between honey samples originating from the hives with different infection levels.The obtained results showed that in hives with the increase of infection, in honey samples the watercontent increased, while the free acidity, the absolute value of the specific optical rotation, and theelectrical conductivity decreased. The higher content of fructose, melbiosis and melezitose wasobserved in honey samples from the hives with a higher degree of infection in comparison with honeysamples from the hives with the lower infection degree. Glucose and isomaltose content was higher inhoney samples originating from the bee colonies less infected compared to honey samples from thehives with the higher infection.In order to investigate variations in the content of plant and bee protein markers in honey,biochemical methods such as electrophoretic analysis and determination of enzyme activity wereapplied. Using sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS PAGE), no significantdifference was found in the protein profile of the analyzed honey samples. Therefore, thespectrophotometric method was applied for determination of the activity of specific enzymes, catalaseand diastase, as plant and bee marker enzymes, respectively. The results of correlation analysis showedthat the colonies’ infestation level was positively correlated with the activity of catalase which can be auseful indicator for screening of honey originating from beehives infested by V. destructor and N.ceranae. On the other hand, no change in diastase activity was found in honey samples obtained frominfected societies.Fluorescence spectroscopy in combination with advanced statistical methods MultivariateCurve Resolution - Alternating Least Squares (MCR-ALS) and Parallel Factor Analysis (PARAFAC)was used to determine the relative protein and phenol content in honey samples...",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Određivanje sadržaja proteina i fenola u uzorcima meda kao indikatora uticaja faktora stresa na pčelinja društva",
url = "https://hdl.handle.net/21.15107/rcub_nardus_21242"
}

Stanković, M.. (2022). Određivanje sadržaja proteina i fenola u uzorcima meda kao indikatora uticaja faktora stresa na pčelinja društva. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_21242

Stanković M. Određivanje sadržaja proteina i fenola u uzorcima meda kao indikatora uticaja faktora stresa na pčelinja društva. in Универзитет у Београду. 2022;.
https://hdl.handle.net/21.15107/rcub_nardus_21242 .

Stanković, Mira, "Određivanje sadržaja proteina i fenola u uzorcima meda kao indikatora uticaja faktora stresa na pčelinja društva" in Универзитет у Београду (2022),
https://hdl.handle.net/21.15107/rcub_nardus_21242 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1759
AB  - INTRODUCTION:
Alginate dressings are widely used in the treatment of exuding wounds29. The enzymatically synthesized lignin model compound dehydrogenate polymer (DHP) from coniferyl alcohol by the enzyme peroxidase, is the best lignin substitute used in
various experiments30. In our previous work, we have shown that synthesized DHP has antibacterial and antibiofilm properties, and in combination with alginate has good potential for wound treatment31.
OBJECTIVES:
The objective of this paper was to study the sustained release of DHP from low and medium viscosity alginate beads.
PB  - Prirodno-matematički fakultet Novi Sad
C3  - International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021
T1  - Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads
EP  - 133
SP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1759
ER  - 

@conference{
author = "Spasojević, Dragica and Stanković, Mira and Prokopijević, Miloš and Prodanović, Olivera and Stojkovska, Jasmina and Obradović, Bojana and Radotić, Ksenija",
year = "2021",
abstract = "INTRODUCTION:
Alginate dressings are widely used in the treatment of exuding wounds29. The enzymatically synthesized lignin model compound dehydrogenate polymer (DHP) from coniferyl alcohol by the enzyme peroxidase, is the best lignin substitute used in
various experiments30. In our previous work, we have shown that synthesized DHP has antibacterial and antibiofilm properties, and in combination with alginate has good potential for wound treatment31.
OBJECTIVES:
The objective of this paper was to study the sustained release of DHP from low and medium viscosity alginate beads.",
publisher = "Prirodno-matematički fakultet Novi Sad",
journal = "International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021",
title = "Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads",
pages = "133-132",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1759"
}

Spasojević, D., Stanković, M., Prokopijević, M., Prodanović, O., Stojkovska, J., Obradović, B.,& Radotić, K.. (2021). Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021
Prirodno-matematički fakultet Novi Sad., 132-133.
https://hdl.handle.net/21.15107/rcub_rimsi_1759

Spasojević D, Stanković M, Prokopijević M, Prodanović O, Stojkovska J, Obradović B, Radotić K. Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021. 2021;:132-133.
https://hdl.handle.net/21.15107/rcub_rimsi_1759 .

Spasojević, Dragica, Stanković, Mira, Prokopijević, Miloš, Prodanović, Olivera, Stojkovska, Jasmina, Obradović, Bojana, Radotić, Ksenija, "Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads" in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021 (2021):132-133,
https://hdl.handle.net/21.15107/rcub_rimsi_1759 .

TY  - JOUR
AU  - Mitrović, Aleksandra Lj.
AU  - Simonović Radosavljević, Jasna
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Kovacević, Jovana
AU  - Prodanović, Olivera
AU  - Todorović, Bratislav
AU  - Matović, Branko
AU  - Stanković, Mira
AU  - Maksimović, Vuk
AU  - Mutavdžić, Dragosav
AU  - Skocic, Milos
AU  - Pesic, Mirjana
AU  - Prokic, Ljiljana
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1449
AB  - The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day-UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C=C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Cell wall response to UV radiation in needles of Picea omorika
EP  - 190
SP  - 176
VL  - 161
DO  - 10.1016/j.plaphy.2021.02.007
ER  - 

@article{
author = "Mitrović, Aleksandra Lj. and Simonović Radosavljević, Jasna and Prokopijević, Miloš and Spasojević, Dragica and Kovacević, Jovana and Prodanović, Olivera and Todorović, Bratislav and Matović, Branko and Stanković, Mira and Maksimović, Vuk and Mutavdžić, Dragosav and Skocic, Milos and Pesic, Mirjana and Prokic, Ljiljana and Radotić, Ksenija",
year = "2021",
abstract = "The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day-UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C=C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Cell wall response to UV radiation in needles of Picea omorika",
pages = "190-176",
volume = "161",
doi = "10.1016/j.plaphy.2021.02.007"
}

Mitrović, A. Lj., Simonović Radosavljević, J., Prokopijević, M., Spasojević, D., Kovacević, J., Prodanović, O., Todorović, B., Matović, B., Stanković, M., Maksimović, V., Mutavdžić, D., Skocic, M., Pesic, M., Prokic, L.,& Radotić, K.. (2021). Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 161, 176-190.
https://doi.org/10.1016/j.plaphy.2021.02.007

Mitrović AL, Simonović Radosavljević J, Prokopijević M, Spasojević D, Kovacević J, Prodanović O, Todorović B, Matović B, Stanković M, Maksimović V, Mutavdžić D, Skocic M, Pesic M, Prokic L, Radotić K. Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry. 2021;161:176-190.
doi:10.1016/j.plaphy.2021.02.007 .

Mitrović, Aleksandra Lj., Simonović Radosavljević, Jasna, Prokopijević, Miloš, Spasojević, Dragica, Kovacević, Jovana, Prodanović, Olivera, Todorović, Bratislav, Matović, Branko, Stanković, Mira, Maksimović, Vuk, Mutavdžić, Dragosav, Skocic, Milos, Pesic, Mirjana, Prokic, Ljiljana, Radotić, Ksenija, "Cell wall response to UV radiation in needles of Picea omorika" in Plant Physiology and Biochemistry, 161 (2021):176-190,
https://doi.org/10.1016/j.plaphy.2021.02.007 . .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1795
AB  - UV-B light, plays a crucial role as a signal for inducing plant response and development of specific photomorphogenic responses. The UV radiation may have a damaging effect on cellular components and macromolecules in seeds, which may influence seed quality. We compared Glycine max L. seeds exposed to UV-B radiation for 1 h with non-irradiated seeds (control). The antioxidant activity was estimated using a DPPH assay. The seeds’ fluorescence characteristics were studied by fluorescence excitation-emission matrices. According to the obtained results the UV-B irradiated seeds possess a significantly higher free radical scavenging activity compared to the control. The fluorescence analysis showed differences in the spectral emission profiles of irradiated seeds compared to the control seeds. The results imply that 1h seed exposure to UV-B increases free radical content which may alter the structures of cellular macromolecules resulting in degradation of some of the fluorophores.
PB  - Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS
EP  - 339
SP  - 336
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1795
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Radotić, Ksenija",
year = "2021",
abstract = "UV-B light, plays a crucial role as a signal for inducing plant response and development of specific photomorphogenic responses. The UV radiation may have a damaging effect on cellular components and macromolecules in seeds, which may influence seed quality. We compared Glycine max L. seeds exposed to UV-B radiation for 1 h with non-irradiated seeds (control). The antioxidant activity was estimated using a DPPH assay. The seeds’ fluorescence characteristics were studied by fluorescence excitation-emission matrices. According to the obtained results the UV-B irradiated seeds possess a significantly higher free radical scavenging activity compared to the control. The fluorescence analysis showed differences in the spectral emission profiles of irradiated seeds compared to the control seeds. The results imply that 1h seed exposure to UV-B increases free radical content which may alter the structures of cellular macromolecules resulting in degradation of some of the fluorophores.",
publisher = "Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS",
pages = "339-336",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1795"
}

Bartolić, D., Stanković, M., Prokopijević, M.,& Radotić, K.. (2021). INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 336-339.
https://hdl.handle.net/21.15107/rcub_rimsi_1795

Bartolić D, Stanković M, Prokopijević M, Radotić K. INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2021;:336-339.
https://hdl.handle.net/21.15107/rcub_rimsi_1795 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Radotić, Ksenija, "INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2021):336-339,
https://hdl.handle.net/21.15107/rcub_rimsi_1795 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Prokopijević, Miloš
AU  - Stanković, Mira
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1762
AB  - Application of fluorescence spectroscopy combined with chemometrics algorithms provides rapid and non-destructive screening method in seed quality estimation, widely used in the agricultural industry and crop breeding. Fluorescence spectroscopy is a technique capable of detecting differs fluorophores among various colored maize seed cultivars and through different seed fractions. In the present study, we used the Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) algorithm to analyse the excitation-emission matrices (EEMs) of various cultivars of colored maize (Zea mays L.) seeds and its fractions. The EEMs were recorded as a set, with the excitation ranging from 280 nm to 330 nm and the emission spectra ranging from 300 nm to 550 nm. The MCR-ALS analysis yielded two major fluorescence components for all of the analysed samples. Both position and shape of component 1 (C1) varied among the samples. On the other hand, the position and shape were similar for component 2 (C2). C1 could be used as a marker for the discrimination of colored seeds and their fractions. The observed variations in C1 between the analysed seeds may be due to the presence of their individual fluorophores, assigned to anthocyanins, proteins, and phenolics. In conclusion, the MCR-ALS analysis of the seed emission spectra has a great potential for the rapid and non-expensive characterization of various cultivars of colored seeds.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina
T1  - Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis
EP  - 746
SP  - 742
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1762
ER  - 

@conference{
author = "Bartolić, Dragana and Prokopijević, Miloš and Stanković, Mira and Radotić, Ksenija",
year = "2021",
abstract = "Application of fluorescence spectroscopy combined with chemometrics algorithms provides rapid and non-destructive screening method in seed quality estimation, widely used in the agricultural industry and crop breeding. Fluorescence spectroscopy is a technique capable of detecting differs fluorophores among various colored maize seed cultivars and through different seed fractions. In the present study, we used the Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) algorithm to analyse the excitation-emission matrices (EEMs) of various cultivars of colored maize (Zea mays L.) seeds and its fractions. The EEMs were recorded as a set, with the excitation ranging from 280 nm to 330 nm and the emission spectra ranging from 300 nm to 550 nm. The MCR-ALS analysis yielded two major fluorescence components for all of the analysed samples. Both position and shape of component 1 (C1) varied among the samples. On the other hand, the position and shape were similar for component 2 (C2). C1 could be used as a marker for the discrimination of colored seeds and their fractions. The observed variations in C1 between the analysed seeds may be due to the presence of their individual fluorophores, assigned to anthocyanins, proteins, and phenolics. In conclusion, the MCR-ALS analysis of the seed emission spectra has a great potential for the rapid and non-expensive characterization of various cultivars of colored seeds.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina",
title = "Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis",
pages = "746-742",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1762"
}

Bartolić, D., Prokopijević, M., Stanković, M.,& Radotić, K.. (2021). Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis. in Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 742-746.
https://hdl.handle.net/21.15107/rcub_rimsi_1762

Bartolić D, Prokopijević M, Stanković M, Radotić K. Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis. in Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina. 2021;:742-746.
https://hdl.handle.net/21.15107/rcub_rimsi_1762 .

Bartolić, Dragana, Prokopijević, Miloš, Stanković, Mira, Radotić, Ksenija, "Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis" in Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina (2021):742-746,
https://hdl.handle.net/21.15107/rcub_rimsi_1762 .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Maksimović, Vuk
AU  - Dragišić Maksimović, Jelena
AU  - Stanković, Mira
AU  - Krstović, Sasa
AU  - Baosic, Rada
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1358
AB  - BACKGROUND Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose-response manner, with a ratio of below 1 up to a concentration of 51.51 mu g kg(-1), and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 mu g kg(-1), respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose-response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship
EP  - 2910
IS  - 7
SP  - 2905
VL  - 100
DO  - 10.1002/jsfa.10317
ER  - 

@article{
author = "Bartolić, Dragana and Maksimović, Vuk and Dragišić Maksimović, Jelena and Stanković, Mira and Krstović, Sasa and Baosic, Rada and Radotić, Ksenija",
year = "2020",
abstract = "BACKGROUND Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose-response manner, with a ratio of below 1 up to a concentration of 51.51 mu g kg(-1), and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 mu g kg(-1), respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose-response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship",
pages = "2910-2905",
number = "7",
volume = "100",
doi = "10.1002/jsfa.10317"
}

Bartolić, D., Maksimović, V., Dragišić Maksimović, J., Stanković, M., Krstović, S., Baosic, R.,& Radotić, K.. (2020). Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 100(7), 2905-2910.
https://doi.org/10.1002/jsfa.10317

Bartolić D, Maksimović V, Dragišić Maksimović J, Stanković M, Krstović S, Baosic R, Radotić K. Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship. in Journal of the Science of Food and Agriculture. 2020;100(7):2905-2910.
doi:10.1002/jsfa.10317 .

Bartolić, Dragana, Maksimović, Vuk, Dragišić Maksimović, Jelena, Stanković, Mira, Krstović, Sasa, Baosic, Rada, Radotić, Ksenija, "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship" in Journal of the Science of Food and Agriculture, 100, no. 7 (2020):2905-2910,
https://doi.org/10.1002/jsfa.10317 . .

TY  - JOUR
AU  - Stanković, Mira
AU  - Nikčević, Miroslav
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1319
AB  - Proteins and phenols are minor, but important components of honey. Phenols originate from nectar and proteins from nectar/pollen and bees themselves. These two types of compounds are important for growth and development of bee colonies, and also for their defense capacity. Their quantity in honey depends on food accessibility and forage during the year. In this preliminary study, we aimed to see yearly dynamics of ratio of phenols and proteins in honey for a bee colony, and to observe the possible effects of extended oxalic acid antivarroa treatment on honey composition regarding proteins and phenols. Since proteins and phenols are fluorescently active, for measurement of phenol/protein ratio in honey we applied steady-state fluorescence spectroscopy as a rapid and non-destructive method. We determined the ratio of the spectral components related to these two main fluorophores in honey, proteins and phenolic compounds, as a ratiometric indicator of variability in honey samples collected in different seasons. We found that oxalic acid does not influence proteins' and phenolics' composition of honey, based on the spectral analysis. Since proteins in honey originate mainly from bees colony, this result indicates that oxalic acid did not change bee synthesis of proteins, indicating that oxalic acid, as a protective agent, is not harmful to the bees.
PB  - Springer, New York
T2  - European Food Research and Technology
T1  - Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition
EP  - 1518
IS  - 7
SP  - 1515
VL  - 246
DO  - 10.1007/s00217-020-03507-x
ER  - 

@article{
author = "Stanković, Mira and Nikčević, Miroslav and Radotić, Ksenija",
year = "2020",
abstract = "Proteins and phenols are minor, but important components of honey. Phenols originate from nectar and proteins from nectar/pollen and bees themselves. These two types of compounds are important for growth and development of bee colonies, and also for their defense capacity. Their quantity in honey depends on food accessibility and forage during the year. In this preliminary study, we aimed to see yearly dynamics of ratio of phenols and proteins in honey for a bee colony, and to observe the possible effects of extended oxalic acid antivarroa treatment on honey composition regarding proteins and phenols. Since proteins and phenols are fluorescently active, for measurement of phenol/protein ratio in honey we applied steady-state fluorescence spectroscopy as a rapid and non-destructive method. We determined the ratio of the spectral components related to these two main fluorophores in honey, proteins and phenolic compounds, as a ratiometric indicator of variability in honey samples collected in different seasons. We found that oxalic acid does not influence proteins' and phenolics' composition of honey, based on the spectral analysis. Since proteins in honey originate mainly from bees colony, this result indicates that oxalic acid did not change bee synthesis of proteins, indicating that oxalic acid, as a protective agent, is not harmful to the bees.",
publisher = "Springer, New York",
journal = "European Food Research and Technology",
title = "Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition",
pages = "1518-1515",
number = "7",
volume = "246",
doi = "10.1007/s00217-020-03507-x"
}

Stanković, M., Nikčević, M.,& Radotić, K.. (2020). Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition. in European Food Research and Technology
Springer, New York., 246(7), 1515-1518.
https://doi.org/10.1007/s00217-020-03507-x

Stanković M, Nikčević M, Radotić K. Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition. in European Food Research and Technology. 2020;246(7):1515-1518.
doi:10.1007/s00217-020-03507-x .

Stanković, Mira, Nikčević, Miroslav, Radotić, Ksenija, "Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition" in European Food Research and Technology, 246, no. 7 (2020):1515-1518,
https://doi.org/10.1007/s00217-020-03507-x . .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Mitrović, Aleksandra Lj.
AU  - Mutavdžić, Dragosav
AU  - Simonović Radosavljević, Jasna
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1794
AB  - Maize seeds are a primary source of nourishment and their viability is a critical consideration to
ensure a reasonably high harvest and seed quality. In this work, we study the effect of aflatoxin
induced stress on the seed viability concerning the germination, as well as the estimation on the
impact on the viability using fluorescence spectroscopy. Our results showed that the contaminated
seeds exhibit significant decreases in the percent of germination, even at low levels, if compared to the
uncontaminated seeds (P < 0.05). Furthermore, the fluorescence ratio of 453/680 and 680/751, could
be used for the rapid screening of the viability of the maize seeds.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 27th International Conference Ecological Truth and Environmental Research
T1  - Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy
EP  - 304
SP  - 301
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1794
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Mitrović, Aleksandra Lj. and Mutavdžić, Dragosav and Simonović Radosavljević, Jasna and Radotić, Ksenija",
year = "2019",
abstract = "Maize seeds are a primary source of nourishment and their viability is a critical consideration to
ensure a reasonably high harvest and seed quality. In this work, we study the effect of aflatoxin
induced stress on the seed viability concerning the germination, as well as the estimation on the
impact on the viability using fluorescence spectroscopy. Our results showed that the contaminated
seeds exhibit significant decreases in the percent of germination, even at low levels, if compared to the
uncontaminated seeds (P < 0.05). Furthermore, the fluorescence ratio of 453/680 and 680/751, could
be used for the rapid screening of the viability of the maize seeds.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "27th International Conference Ecological Truth and Environmental Research",
title = "Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy",
pages = "304-301",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1794"
}

Bartolić, D., Stanković, M., Mitrović, A. Lj., Mutavdžić, D., Simonović Radosavljević, J.,& Radotić, K.. (2019). Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy. in 27th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 301-304.
https://hdl.handle.net/21.15107/rcub_rimsi_1794

Bartolić D, Stanković M, Mitrović AL, Mutavdžić D, Simonović Radosavljević J, Radotić K. Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy. in 27th International Conference Ecological Truth and Environmental Research. 2019;:301-304.
https://hdl.handle.net/21.15107/rcub_rimsi_1794 .

Bartolić, Dragana, Stanković, Mira, Mitrović, Aleksandra Lj., Mutavdžić, Dragosav, Simonović Radosavljević, Jasna, Radotić, Ksenija, "Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy" in 27th International Conference Ecological Truth and Environmental Research (2019):301-304,
https://hdl.handle.net/21.15107/rcub_rimsi_1794 .