TY  - JOUR
AU  - Radotić, Ksenija
AU  - Melø, Thor B.
AU  - Lindgren, Mikael
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2201
AB  - In situ fluorescence measurements have been used to investigate relative amounts of blue-green pigments and
their distributions in plant leaves from Euphorbia pulcherrima. Advantage was taken from the fact that this species
has white leaves on the top, with low pigment concentrations, and green leaves on the stem with ordinary
pigment concentrations. Excitation- and emission spectra below 410 nm from white leaves, where pigment
absorption is low, are not distorted by self-absorption. Absorption- and reflection spectra from white and green
leaves were measured using a spectrophotometer equipped with an integrating sphere. The absorption spectra
were used to correct recorded fluorescence spectra for self-absorption. Self-absorption corrected photosystem
fluorescence from green leaves, modeling light transmission in leaf tissue exponentially, matches to the excitation/
emission spectra from white leaves, apart from small differences due to the pigment concentrations and
selective scattering. The introduced exponentially decaying transmission relation also predicts that the ratio of
excitation spectra from a white and green leaf is in proportion to the absorption spectrum of the green leaf, which
was validated for Photosystem II particle fluorescence. This relation was also used to find a scaled absorption
spectrum responsible for blue-green emission, which was assumed to originate from lignin. Excitation/emission
spectra of the blue-green fluorescence were decomposed into five components and their relative amounts from
adaxial and abaxial sides of the leaves have been quantified. Fluorescence lifetime measurements of the leaves,
PB  - Elsevier
T2  - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
T1  - A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)
EP  - 8
IS  - 123269
SP  - 2
VL  - 303
DO  - 10.1016/j.saa.2023.123269
ER  - 

@article{
author = "Radotić, Ksenija and Melø, Thor B. and Lindgren, Mikael",
year = "2023",
abstract = "In situ fluorescence measurements have been used to investigate relative amounts of blue-green pigments and
their distributions in plant leaves from Euphorbia pulcherrima. Advantage was taken from the fact that this species
has white leaves on the top, with low pigment concentrations, and green leaves on the stem with ordinary
pigment concentrations. Excitation- and emission spectra below 410 nm from white leaves, where pigment
absorption is low, are not distorted by self-absorption. Absorption- and reflection spectra from white and green
leaves were measured using a spectrophotometer equipped with an integrating sphere. The absorption spectra
were used to correct recorded fluorescence spectra for self-absorption. Self-absorption corrected photosystem
fluorescence from green leaves, modeling light transmission in leaf tissue exponentially, matches to the excitation/
emission spectra from white leaves, apart from small differences due to the pigment concentrations and
selective scattering. The introduced exponentially decaying transmission relation also predicts that the ratio of
excitation spectra from a white and green leaf is in proportion to the absorption spectrum of the green leaf, which
was validated for Photosystem II particle fluorescence. This relation was also used to find a scaled absorption
spectrum responsible for blue-green emission, which was assumed to originate from lignin. Excitation/emission
spectra of the blue-green fluorescence were decomposed into five components and their relative amounts from
adaxial and abaxial sides of the leaves have been quantified. Fluorescence lifetime measurements of the leaves,",
publisher = "Elsevier",
journal = "Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy",
title = "A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)",
pages = "8-2",
number = "123269",
volume = "303",
doi = "10.1016/j.saa.2023.123269"
}

Radotić, K., Melø, T. B.,& Lindgren, M.. (2023). A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima). in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Elsevier., 303(123269), 2-8.
https://doi.org/10.1016/j.saa.2023.123269

Radotić K, Melø TB, Lindgren M. A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima). in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2023;303(123269):2-8.
doi:10.1016/j.saa.2023.123269 .

Radotić, Ksenija, Melø, Thor B., Lindgren, Mikael, "A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)" in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 303, no. 123269 (2023):2-8,
https://doi.org/10.1016/j.saa.2023.123269 . .